Abstract: The present invention relates to a process for treating an oil comprising a chlorophyll substrate, the process comprising contacting the oil with a polypeptide having decolorase activity or a composition comprising the polypeptide, wherein the polypeptide is selected from the group consisting of: a. a polypeptide which has at least 80% identity to amino acids 1 to 318 of SEQ ID NO: 1; and, b. a polypeptide encoded by a nucleic acid sequence that has at least 80% identity to the nucleic acid sequence of SEQ ID NO: 2.

Abstract: The present invention provides a process for the enzymatic degumming of unrefined triglyceride oils, said process comprising: a) providing an unrefined triglyceride oil having a phosphorus content of at least 100 mg per kg of unrefined triglyceride oil; b) combining the unrefined triglyceride oil with water, lactic acid and enzyme to produce an oil-and-water emulsion, said enzyme being selected from phospholipase, lipid acyltransferase and combinations thereof; c) keeping the emulsion at a temperature of 20-90° C. for at least 10 minutes; and d) separating degummed triglyceride oil from the emulsion. This enzymatic degumming process is extremely effective in removing phospholipids, including non-hydratable phospholipids (NHP), from crude and other unrefined vegetable oils.

Abstract: Provided are methods and systems of propagating a microorganism on a stillage composition. The methods involve growing microorganisms in a propagation medium formed from a polysaccharide-containing stillage composition with the majority of the of the polysaccharides in the propagation medium coming from the stillage composition. The propagation medium also includes cellulases and/or amylases to form monosaccharides from the polysaccharides. A first cell mass is grown in the propagation medium to form a second cell mass which is greater than the first cell mass.

Abstract: Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

Abstract: Methods of preserving and storing biological specimens, particularly anaerobe microorganisms are disclosed. A composition containing oxygen scavenging membrane fragments and a cryoprotectant is administered to the specimens. The specimens are then stored at a temperature that is lower than 0° C. and greater than ?70° C. Cryogenic freezing is not required.

Abstract: The present invention relates to a process for degumming a vegetable oil, comprising a. contacting an oil-water mixture comprising a crude vegetable oil comprising phospholipids with an enzyme having a phospholipase activity, wherein the oil-water mixture comprises an aqueous solution having a molal ionic strength of between 0.001 and 0.5 mol/kg; b. separating an oil-water mixture into an oil composition and an aqueous composition; and, c. washing the oil composition with an acid, wherein a degummed vegetable is produced.

Abstract: A novel hybrid corn plant, designated A7882 is disclosed. The invention relates to the seeds of hybrid corn A7882, to the plants and plant parts of hybrid corn A7882, and to methods for producing a corn plant by crossing the hybrid corn A7882 with itself or another corn plant. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other hybrid corn plants derived from the hybrid corn plant A7882.

Abstract: The present invention relates to a polypeptide comprising (a) a first set of six complementarity determining regions (CDRs) configured to bind a first antigen; and (b) (ba) a second set of six CDRs configured to bind a second antigen; or (bb) a ligand capable of binding to a second antigen; wherein (i) said first antigen is selected from Hepatitis B virus (HBV) small surface antigen; HBV medium surface antigen; and HBV large surface antigen; and (ii) said second antigen is selected from surface antigens presented by immune effector cells such as natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). Also provided are compositions for use in a method of treating or preventing HBV infection and/or a condition caused by said HBV infection, said condition caused by said HBV infection being selected from liver cirrhosis and hepatocellular carcinoma.

Abstract: A method for obtaining natural extracts, oleoresins, colorants flavors and aromas from aromatic plant substances, alfalfa, flowers with pigments, and vegetables, which includes washing the plant substances and vegetables, rinsing with water, milling and sifting the resulting product yielding a pulp phase and a cellulose phase. The pulp phase is transferred to fermentation tanks and a first centrifugation of the fermented pulp phase is then performed yielding two phases: phase (A) containing water, mineral salts and other water-soluble substances, with natural extracts, oleoresins, colorants, aromas and flavors and phase (B) in the form of a paste in which the moisture has been reduced to 50%, performing a second centrifugation of the mentioned phase (A), which causes the separation of natural extracts, oleoresins, colorants, aromas and flavors from the water and other water-soluble substances.

Abstract: A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.

Abstract: A method for lowering content of a sterol in a highly unsaturated fatty acid-concentrated oil in a method to concentrate a highly unsaturated fatty acid by using a lipase reaction, may include removing the free form of the sterol from a raw material oil that includes a highly unsaturated fatty acid-containing glyceride prior to performing the lipase reaction; and thereafter concentrating the highly unsaturated fatty acid in the glyceride by using a lipase that is less reactive for the highly unsaturated fatty acid.

Abstract: Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme and methods for recovering lipids from the cells.

Abstract: The invention relates to a composition comprising at least one phospholipid-cleaving enzyme. The invention further relates to a method for degumming raw oils using the composition according to the invention and to the use of the composition according to the invention to degum raw oils.

Abstract: In one aspect, there is provided a process for treating a plant oil, comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolysing an a? or b? stereoisomer of chlorophyll or a chlorophyll derivative.

Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like.

Abstract: A process for production of biofuels from algae can include cultivating an oil-producing algae, extracting the algal oil, and converting the algal oil to form biodiesel. Extracting the algal oil from the oil-producing algae can include biologically rupturing cell wall and oil vesicles of the oil-producing algae using at least one enzyme such as a cellulose or glycoproteinase, a structured enzyme system such as a cellulosome, a virus, or combination of these materials.

Abstract: Thermostable enzyme technology for algal bioconversion The present invention relates to thermostable enzyme systems suitable for use in the production of biofuels and bio-products from algae, and to a method of producing energy feedstocks, stocks, specifically (i) fermentable sugars and (ii) lipid fractions from algae, for the production of biofuels such as bioethanol, biobutanol and bio-oils or biodiesel, as well as other value-added biomolecules (e.g. proteins, peptides, oils, pigments, nucleic acids).

Abstract: The invention relates to a method for recovering of oil from plant seeds characterized in that a) an aqueous solution containing one or more cellulolytic and/or lipolytic and/or pectinolytic and/or proteolytic enzyme(s) and/or phytase is sprayed onto the seed, b) the thus obtained seed is directly supplied to a one-stage or multistage pressing in a way known per se, optionally coupled to an extraction, and c) the oil is recovered in a way known per se and optionally further processed, and the use of the method, particularly in the production of edible oil or biodiesel.

Abstract: The present invention relates to a biphasic microalgae extract comprising 15-95 wt. % of an aqueous phase and 5-85 wt. % of an oil phase, said extract containing at least 1% by weight of total fatty acids of ?3-fatty acids selected from the group consisting of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and combinations thereof, said extract further being characterized in that it contains: • 40-100% by weight of the oil phase of lipids selected from triglycerides, diglycerides, monoglycerides, phosphatides, free fatty acids and combinations thereof; • 0.01-10% of carotenoids by weight of the oil phase; • 0.5-10% of sodium chloride by weight of the aqueous phase; • 0-3 wt. % of non-dissolved material other than dispersed droplets of aqueous phase or oil phase; and • 0-10% of C1-5 monoalcohol by weight of the aqueous phase. Together the aqueous phase and oil phase of the biphasic extract contain essentially all the lipid material that was originally contained in the microalgae biomass, i.e.

Abstract: Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae.

Abstract: Provided herein are compositions and methods for enhancing enzyme activity, half-life and/or thermostability. Also provided herein are compositions and methods including the enhanced enzymes. Also provided herein are methods and compositions related to improved pectinolytic enzymes, such as pectate lyase, which exhibit enhanced activity, thermostability and/or longer half-life.

Abstract: A method for extracting oil from sea cucumber material. The sea cucumber material is mixed with water and the mixture is heated at a predetermined heating temperature for a predetermined heating time interval. The pH value and temperature of the mixture are adjusted to be compatible with an enzyme. A predetermined amount of the enzyme is then added to the mixture. The mixture is incubated at a predetermined incubation temperature for a predetermined incubation time interval. After the incubation the oil is removed from the mixture.

Abstract: A method for processing of byproducts in an ethanol production process to improve their nutritional value is provided. The method includes applying a mixture of microorganism to a stillage stream with concomitant production of organic acids and de-emulsification and liberation of oil for recovery. An improved livestock feed resulting from ethanol production is provided which comprises a much reduced oil content and a much higher level of organic acids.

Abstract: The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials.

Abstract: In one aspect of the invention, a method recovers oil from a concentrated byproduct, such as evaporated thin stillage formed during a dry milling process used for producing ethanol. The method includes forming a concentrate from the byproduct and recovering oil from the concentrate. The step of forming the concentrate may comprise evaporating the byproduct. Further, the step of separating the oil from the concentrate may comprise using a centrifuge and, in particular, a disk stack centrifuge. Other aspects of the invention include related methods and subsystems for recovering oil from thin stillage.

Abstract: A method for lowering content of a sterol in a highly unsaturated fatty acid-concentrated oil in a method to concentrate a highly unsaturated fatty acid by using a lipase reaction, may include removing the free form of the sterol from a raw material oil that includes a highly unsaturated fatty acid-containing glyceride prior to performing the lipase reaction; and thereafter concentrating the highly unsaturated fatty acid in the glyceride by using a lipase that is less reactive for the highly unsaturated fatty acid.

Abstract: A Capture Carbon Storage (CCS) Process for the economical capture of carbon dioxide from coal fuel gas, and the storage of the carbon dioxide as lipid oil or the use of the environmentally begin oil for transportation. The lipid oil may be refined into gasoline, biodiesel fuel, jet fuel, ethanol, and methane which provide a renewable energy resource for the United State's future energy needs. It is a new fuel form which has been both chemically and physically altered to reduce the emissions of carbon dioxide. It is a coal cleaning/upgrading process which produces a refined fuel, Hydrogen, that may be used to produce electricity and an environmentally begin biofuel for transportation. The renewable fuel will produce no carbon foot print at the internal combustion engine's exhaust pipe. The CCS Process will produce a 100% reduction in carbon dioxide (CO2) emissions into the atmosphere and thereby, stop the Global Warming.

Abstract: This invention relates to krill oil and a method for manufacturing the same. The method comprises preparing krill, adding protease to the krill and performing enzyme reaction, extracting eicosapentaenoic acid, docosahexaenoic acid, phospholipids, and astaxanthin from the krill after performing the enzyme reaction, and mixing the extracted eicosapentaenoic acid, docosahexaenoic acid, phospholipids, and astaxanthin. The protease may comprise one or more chosen from serine alkaline proteases and metallo neutral proteases. The serine alkaline proteases may comprise proteases separated from Bacillus licheniformis and the metallo neutral proteases may comprise proteases separated from Bacillus subtillis and Bacillus amyloliquefaciens.

Abstract: The development and application of a novel non-polar oil recovery process utilizing a non-dispersive solvent extraction method to coalesce and recover oil from a bio-cellular aqueous slurry is described herein. The process could apply to recovery of algal oil from a lysed or non-lysed algae slurry, recovery of Omega fatty acids from a bio-cellular aqueous feed, recovery of Beta-carotene from a bio-cellular aqueous feed and for the removal from produced water in oil production and similar type applications. The technique of the present invention utilizes a microporous hollow fiber (MHF) membrane contactor. The non-polar oil recovery process described herein can be coupled to a collecting fluid (a non-polar solvent such as heptane, a biodiesel mixture or the previously extracted oil) that is circulated through the hollow fiber membrane. In cases where the biodiesel mixture or the previously extracted oil is used the solvent recovery step (e.g. distillation) can be eliminated.

Abstract: The technology disclosed herein relates to novel methods and compositions for the production processes of algae cell components from algae of the genus Scenedesmus. The extractability of algae cellular components is improved and the process is optimized and accelerated by using the enzyme compositions according to the present invention.

Abstract: The present invention provides a process for the extraction of lipids from microbial lipid-containing feedstock, comprising the steps of (a) subjecting the microbial lipid-containing feedstock to a treatment to release the lipids thereby producing a mixture; (b) subjecting the mixture obtained in (a) to a filtration in the presence of a first extraction solvent on a filter, wherein organic matter soluble in an extraction solvent and an aqueous mixture are removed as filtrate from a retentate comprising non-soluble matter; (c) separating the filtrate obtained from the filter into an organic and aqueous phase; and (d) subjecting the organic phase to a distillation treatment to separate solvent and an organic residue comprising extracted lipids.

Abstract: In one aspect, there is provided a process for treating a plant oil, comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolysing an a? or b? stereoisomer of chlorophyll or a chlorophyll derivative.

Abstract: The invention provides methods of cultivating microalgae photoautotrophically outdoors to prepare concentrated microalgae products containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) docosahexaenoic acid, two long-chain polyunsaturated fatty acids found in fish oil that are very important for human and animal health. It also provides concentrated microalgae products containing EPA and DHA and purified lipid products containing EPA and DHA purified from microalgae.

Abstract: A method for processing of byproducts in an ethanol production process is provided. The method includes applying a mixture of a microorganism to a stillage stream, metabolizing gums in the stillage stream using the mixture to release oil in the gums carried by the stillage stream and recovering the oil. Methods of liberating or releasing bound oil present in stillage are also provided.

Abstract: A method of extracting oil from algae by drying algae paste to a predetermined moisture content, contacting the algae paste with a polar solvent to make an algae-solvent solution and extracting oils from the algae paste into a solvent-oil solution, and separating extracted algae from the solvent-oil solution. An oil of whole and unhydrolyzed phospholipids, whole and unhydrolyzed glycolipids, lysolipids, and carotenoids extracted by the above method. An omega-3 fatty acid of docosahexanoic acids (DHAs) and eicosapentaenoic acids (EPAs) extracted from the above method. Isolated nutraceutical grade and pharmaceutical grade oil derived from algae and being free of toxins extracted by the above method. Isolated oil derived from algae including at least one omega-3 fatty acid of DHA and EPA at least partially in the form of whole and unhydrolyzed phospholipids and whole and unhydrolyzed glycolipids extracted by the above method.

Abstract: The present invention relates to isolated polypeptides having phospholipase C activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A method of producing a refined, bleached and deodorised vegetable oil comprises degumming a vegetable oil, bleaching the degummed oil and deodorising the bleached oil, wherein the degumming, bleaching and deodorising conditions are selected to reduce the content of 3-chloropropane-1,2-diol and esters thereof in the deodorised oil to less than 8 ppm.

Abstract: Phytoplankton represent a potential source of bioactive compounds. The present disclosure provides, inter alia, methods for identifying glycerolipids and apoptosis-inducing sphingosine-like lipids from virally-infected phytoplankton.

Abstract: A method for the enzymatic breakdown of phosphorus-containing constituents and glycosides, in particular steryl glycoside, from crude oil or pre-degummed oil of vegetable or animal origin, wherein for both cases the addition of the enzymes is effected in one method step.

Abstract: A process for separating polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and eicosapen-taenoic acid (EPA) from less saturated long chain fatty acids (LCFAs) in a lipid composition, wherein said PUFAs and LCFAs are present as (i) triglycerides, or (ii) free fatty acids or monoalkyl esters, by exchange of at least a portion of LCFAs with short and/or medium chain fatty acids (MCFAs). The process can suitably be employed on marine derived oil, marine oil 2 derived oil products and other sources of PUFAs, including PUFA-rich singe cell oils (SCOs), and oils from genetically modified organisms with a modified lipid metabolism. The inventive process is based on novel use of lipases and distillation techniques, selectively chemically modifying species in the substrate material such that the desired species and chemically similar species become sufficiently dissimilar to be separable.

Abstract: A phospholipase-phospholipase-carrier complex for degumming crude oils. The phospholipase-carrier complex includes at least one phospholipase A1, A2 or B and at least one carrier, such as silicic acid, precipitated silicic acid, polyacrylate, polymethacrylate or divinylbenzene-crosslinked methacrylate. The disclosure further relates to a method of degumming crude oils and to a method of producing the phospholipase-carrier complex.

Abstract: Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme and methods for recovering lipids from the cells.

Abstract: Process wherein the fatty acid residues on a glyceride moiety are randomised over the terminal and middle positions, wherein the process proceeds to a conversion degree on the terminal positions, Re, ranging from 0.3 to 0.95, and wherein a conversion degree on the middle position, Ra, ranges from 0.06 to 0.75, and wherein Ra is greater than 0.32Re-0.08, the process comprises the exposure of a triglyceride fat to a catalyst comprising a lipase wherein the lipase is a Thermomyces lanuginosa lipase which has an activity of at least 250 IUN corresponding to 22 g/(g*h) at the onset of the process.

Abstract: In one aspect the present invention provides a process for treating oil-containing seeds, comprising a step of contacting the seeds with an enzyme which is capable of hydrolysing chlorophyll or a chlorophyll derivative. Also provided are a method for obtaining oil from plant seeds and a process for producing a refined plant oil comprising such a treatment. Further provided are crude and refined plant oils obtainable from the processes and methods.

Abstract: In one aspect, provided herein is a process for refining a plant oil, comprising a step of contacting the oil with an enzyme which is capable of hydrolysing chlorophyll or a chlorophyll derivative, wherein the enzyme is contacted with the oil in the presence of at least 0.1% by weight phospholipid.

Abstract: Described herein are methods, devices, and compositions for fractionation and processing of microparticles from biological samples, and to methods for obtaining and using the microparticles for biomarker discovery. Biological samples include cell-free fluids, for example blood plasma, blood serum, cerebrospinal fluid, urine, and saliva, as well as conditioned media. Conditioned media is the liquid growth media used to propagate cells in vitro. Purification of microparticles from cell-free fluids is challenging, typically accomplished by prolonged ultracentrifugation. Described herein is an alternative method for efficiently harvesting and processing microparticles from cell-free fluids and from conditioned media. Embodiments described herein relate to use of the microparticles and their contents recovered from conditioned media derived from propagation of human and animal cells, as a source of biomarkers for diagnosis and prognosis of diseases and pathological conditions.

Abstract: In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins.

Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

Abstract: A process for producing triglycerides which comprises: (a) subjecting a first triglyceride comprising at least 40% by moles of oleic acid residues, based on total acyl groups in the triglyceride, to an alcoholysis reaction with an alcohol having from 1 to 6 carbon atoms to obtain a composition comprising 2-oleoyl monoglyceride and at least one acyl ester of said alcohol; (b) reacting the 2-oleoyl monoglyceride with an acylating agent comprising at least one C12 to C24 saturated fatty acid, at least one ester of said fatty acid or a mixture thereof, to obtain a 1,3-saturated fatty acid acyl 2-oleoyl glyceride; and (c) separating at least a part of the at least one acyl ester after or during step (a) or step (b); (d) reacting the acyl ester with a second glyceride to form 1,3-dioleoyl 2-palmitoyl glyceride.

Abstract: The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.