Sugar (e.g., Molasses Treatment, Etc.) Patents (Class 435/276)
  • Patent number: 9241509
    Abstract: Calories in a juice can be reduced by selectively removing more sucrose than primary sugars, for example, glucose and fructose. An acceptable flavor profile can be achieved since the primary sugars have a higher perceived sweetness than sucrose. The food product processing system for reducing calories can include multiple stages for filtering the juice to produce a clarified low-calorie juice having an acceptable flavor profile.
    Type: Grant
    Filed: March 6, 2009
    Date of Patent: January 26, 2016
    Assignee: Tropicana Products, Inc.
    Inventors: Girish Subramaniam, Teodoro Rivera, Nicholas Shields, Diana Pesha
  • Patent number: 9220291
    Abstract: Calories in a juice can be reduced by selectively removing more sucrose than primary sugars, for example, glucose and fructose. An acceptable flavor profile can be achieved since the primary sugars have a higher perceived sweetness than sucrose. The food product processing system for reducing calories can include multiple stages for filtering the juice to produce a clarified low-calorie juice having an acceptable flavor profile.
    Type: Grant
    Filed: September 30, 2006
    Date of Patent: December 29, 2015
    Assignee: Tropicana Products, Inc.
    Inventors: Girish Subramaniam, Teodoro Rivera, Nicholas Shields, Diana Pesha
  • Publication number: 20150118736
    Abstract: There is provided a method of degrading a heterocyclic aldehyde compound comprising the step of treating the heterocyclic aldehyde compound with Enterobacter sp. microorganisms. There is also provided a method of degrading a carboxylic acid compound comprising the step of treating the carboxylic acid compound with Bacillus sp. microorganisms.
    Type: Application
    Filed: April 29, 2013
    Publication date: April 30, 2015
    Inventors: Jin Chuan Wu, Dongxu Zhang, Yee Ong
  • Publication number: 20150079660
    Abstract: The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variant thereof. AmyE or its variants thereof may be used to eliminate or reduce the iodine-positive starch presented in saccharide liquor.
    Type: Application
    Filed: September 23, 2014
    Publication date: March 19, 2015
    Inventors: Vivek Sharma, Jayarama Shetty, Bruce Strohm
  • Patent number: 8815571
    Abstract: Novel fiber processing methods and the products obtained therefrom are disclosed. Methods may include thermochemical and/or enzymatic hydrolysis of fiber feedstocks including distillers' dried grains, distillers' dried grains with solubles, soyhull, miscanthus and switchgrass. Enzymatic hydrolysis includes hydrolysis with cellulase, hemicellulase, and protease.
    Type: Grant
    Filed: October 8, 2008
    Date of Patent: August 26, 2014
    Assignee: Archer Daniels Midland Co.
    Inventors: Charles A. Abbas, Wu-Li Bao
  • Publication number: 20120190092
    Abstract: Methods for enzymatically isolating lignin and other bioproducts, such as fermentable sugars, from herbaceous plant materials, are described. The methods can provide improvements, such as increased product purity and reduced process energy requirements and product modifications and contamination. Systems for practicing the methods also are provided.
    Type: Application
    Filed: January 17, 2012
    Publication date: July 26, 2012
    Applicant: BUCKMAN LABORATORIES INTERNATIONAL, INC.
    Inventors: Percy Jaquess, Bernard Janse
  • Publication number: 20120107920
    Abstract: To achieve efficient use of enzyme in obtaining a sugar solution through a reaction of the enzyme and biomass containing cellulose. In obtaining a sugar solution through a reaction of enzyme and biomass containing cellulose, the biomass and the enzyme are caused to react in a first reaction tank, whereby a sugar solution in which the enzyme is dispersed and a residue containing the unreached biomass adsorbing the enzyme are generated, these sugar solution and residue are next separated, a pH adjusting solution is supplied to the residue in a second reaction tank to prepare a dilute solution whose sugar concentration is lower than that of the sugar solution, and in this dilute solution, a sugar solution is generated through a reaction between the residue and the enzyme adsorbed to the residue.
    Type: Application
    Filed: July 15, 2010
    Publication date: May 3, 2012
    Applicant: JGC CORPORATION
    Inventors: Daisuke Taneda, Yoshiki Ueno, Hirokazu Konishi, Makoto Ikeo
  • Publication number: 20110229956
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Application
    Filed: May 13, 2011
    Publication date: September 22, 2011
    Applicants: Danisco US Inc., Genencor International, Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Patent number: 7666633
    Abstract: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.
    Type: Grant
    Filed: April 6, 2006
    Date of Patent: February 23, 2010
    Inventors: Walter Callen, Toby Richardson, Gerhard Frey, Carl Miller, Martin Kazaoka, Jay M. Short, Eric J. Mathur
  • Patent number: 7560126
    Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.
    Type: Grant
    Filed: March 6, 2003
    Date of Patent: July 14, 2009
    Assignee: Verenium Corporation
    Inventors: Walter Callen, Toby Richardson, Gerhard Frey, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska, Nelson R. Barton, Eileen O'Donoghue, Eric J. Mathur, Jay M. Short
  • Publication number: 20090176297
    Abstract: A method for pretreating and disrupting cell structure of biomass by subjecting the biomass to high pressure, thereby disrupting the cell structure of the biomass is provided. Also provided is a device for performing the method, wherein the device includes a cavitating device and a cell structure disrupting device disposed within the cavitating device for disrupting the cell structure and exposing the internal cell structure to enzymes.
    Type: Application
    Filed: October 11, 2006
    Publication date: July 9, 2009
    Applicant: RA ENERGY CORPORATION
    Inventor: Earnest Stuart
  • Publication number: 20090151026
    Abstract: The present invention relates to isolated polypeptides having feruloyl esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: December 3, 2008
    Publication date: June 11, 2009
    Applicant: Novozymes A/S
    Inventors: Michelle Maranta, Kimberly Brown
  • Publication number: 20090098638
    Abstract: Novel fiber processing methods and the products obtained therefrom are disclosed. Methods may include thermochemical and/or enzymatic hydrolysis of fiber feedstocks including distillers' dried grains, distillers' dried grains with solubles, soyhull, miscanthus and switchgrass. Enzymatic hydrolysis includes hydrolysis with cellulase, hemicellulase, and protease.
    Type: Application
    Filed: October 8, 2008
    Publication date: April 16, 2009
    Inventors: Charles A. Abbas, Wu-Li Bao
  • Patent number: 7238351
    Abstract: The method of producing a microbial adherence inhibitor for administration to food animals to control the incidents of acidosis in food animals by preventing the adherence of colony-forming lactic acid producing bacteria in the rumen or intestinal tracts of the food animals. The method includes inoculating female birds in or about their egg laying age with a lactic acid producing bacteria that colonizes in the intestinal tract of the food animal to be treated. Allowing a period of time sufficient to permit the production in the birds of antibody to the lactic acid producing bacteria and harvesting the eggs laid by such birds. The antibody-containing contents of the eggs are then separated by the shells.
    Type: Grant
    Filed: September 8, 2003
    Date of Patent: July 3, 2007
    Inventors: Peter Nash, Bradley M. Mitteness
  • Patent number: 7077953
    Abstract: An acid recovery system used in a hydrolysis operation includes a chromatographic unit to provide initial separation of sugar and acid. The sugar product provided by the chromatographic unit is processed to produce higher value products, such as ethanol. The remaining acid is contaminated by sugar. A nanofiltration unit containing a nanofilter membrane processes the sugar contaminated acid. The acid is allowed to permeate across the nanofilter membrane while sugar is rejected. The permeate is provided to a conventional acid recovery system and recycled for use in the hydrolysis process.
    Type: Grant
    Filed: September 11, 2003
    Date of Patent: July 18, 2006
    Assignee: Harris Group, Inc.
    Inventor: Jeffrey T. Ranney
  • Patent number: 6812010
    Abstract: The invention concerns a method for enzymatic preparation of homogentisate (HMO) from 4-hydroxypyruvate (HPP), characterized in that it consists in carrying out in a suitable reaction medium the following enzymatic reactions: enzymatic conversion of HPP into 4-hydroxyphenylacetate (HPA) with a first appropriate enzyme; then an enzymatic conversion of HPA into HMO with a second appropriate enzyme.
    Type: Grant
    Filed: October 13, 2000
    Date of Patent: November 2, 2004
    Assignee: Aventis Cropscience SA
    Inventors: Richard Derose, Alain Sailland
  • Patent number: 6627430
    Abstract: The present invention relates to a process for the cultivation of chitin/chirosanrich, filamentous fungi with capacity to assimilate mannose as well as xylose, galactose, and glucose, wherein the cultivation of the filamentous fungi in a liquid medium extracted from wood in the production of paper whereupon the said filamentous fungi are recovered from the medium.
    Type: Grant
    Filed: October 15, 2001
    Date of Patent: September 30, 2003
    Inventor: Lars Edebo
  • Patent number: 6623750
    Abstract: Enzyme feed supplements containing fungal cellulase produced by Trichoderma viride are specially adapted for increasing protein digestability of dairy cow and cattle feed. Preferred embodiments of the present invention include enzyme formulations adapted for application to corn and enzyme formulations adapted for application to grain sorghum, to which is added Trichoderma viride cellulase. An increase in protein digestability has been evidenced by increased protein content of milk and increased nitrogen retention from the feed.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: September 23, 2003
    Assignees: Loveland Industries, Inc., George A. Jeffreys & Co., Inc.
    Inventors: Charles W. Cobb, James F. Tobey
  • Patent number: 6231765
    Abstract: A process for obtaining a polymeric complex from a by-product effluent obtained in a process for manufacturing alcohol by distilling a fermented aqueous sugar solution containing yeast. After distilling alcohol from the effluent a chemical or biological flocculant is added to the effluent to form a homogeneous mixture, which is heated to a temperature of at least about 80° C. Yeast is removed from the heated mixture by a first decantation, then insoluble solids and muds are removed by a second decantation. The mixture is then concentrated by partially evaporating water therefrom to obtain an aqueous solution of the polymeric complex.
    Type: Grant
    Filed: June 24, 1999
    Date of Patent: May 15, 2001
    Inventor: Luis Perez Barrenechea
  • Patent number: 6156563
    Abstract: A new method for clarifying sugar cane juice is disclosed. Treating the phytic acid (or phytate) in sugar cane juice with phytase and acid phosphatase converts the phytic acid to inositol and inorganic phosphate, thereby improving the clarification of the sugar juice substantially. The valuable product inositol results as a consequence of the hydrolysis of phytic acid. The process is environmentally friendly, as it requires only the addition of small amounts of enzymes to existing sugar clarification processes. It does not cause inversion of sucrose to other sugars, and does not cause color formation. The extra phosphate released by this process is available to react with milk of lime, thus improving subsequent flocculation and consequent clarification.
    Type: Grant
    Filed: January 25, 1999
    Date of Patent: December 5, 2000
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventor: Willem H. Kampen
  • Patent number: 6156543
    Abstract: Process for preparing surfactant, which comprises contacting cane trash, maize by-products, sorghum by-products, barley by-products, rice by-products, fruits, chicory pulp, tubers or cynara for at least 5 seconds with a hydrolysing agent selected from an aqueous acid solution at between 20 and 150.degree. C. and an enzymatic hydrolysing composition of a plant material at between 20 and 90.degree. C. to obtain a sugar syrup, freeing the sugar syrup from any solid residues and contacting the residue-free sugar syrup with a C.sub.4-22 -alcohol at a temperature of between 20 and 150.degree. C., preferably between 30 and 110.degree. C., until a solution of surfactant glycosides is obtained, and separating the surfactant glycosides from this solution.
    Type: Grant
    Filed: August 7, 1998
    Date of Patent: December 5, 2000
    Assignee: Agro Industrie Recherches et Developpements (A.R.D.)
    Inventors: Jean-Noel Bertho, Philippe Mathaly, Veronique Dubois, Regis de Baynast de Septfontaines
  • Patent number: 6106673
    Abstract: A process and system are provided for the separation of a fermentation process byproduct into its constituent components and for the subsequent recovery of those constituent components. The process is remarkably simple--requiring only 1) heating of a mixture containing the byproduct so as to separate the oil from a base component of the byproduct to which the oil is bound, followed by 2) recovering the base product, oil, and possibly other substances such as molasses from the mixture. The process can be performed on a large scale and in a continuous fashion using a mechanical separator to recover fibers from a heated mixture to produce a solids stream and a liquor stream and by then removing oil and insoluble substances from the liquor stream in an evaporator assembly.
    Type: Grant
    Filed: January 8, 1998
    Date of Patent: August 22, 2000
    Assignee: CDC Environmental Corp.
    Inventor: David Ray Walker
  • Patent number: 6077702
    Abstract: A glucanase enzyme is described. In addition, there is described a nucleotide sequence coding for the glucanase enzyme and a promoter for controlling its expression.
    Type: Grant
    Filed: April 6, 1998
    Date of Patent: June 20, 2000
    Assignee: Danisco A/S
    Inventors: Susan Madrid, Preben Rasmussen, Anita Baruch
  • Patent number: 6017753
    Abstract: A process for decolorizing an aqueous solution of methyl glucoside, the solution containing at least one color component and at least one sugar component, is disclosed. The process comprises the steps of providing the aqueous solution of MeG; adding to said solution an amount of yeast or other sugar-converting microorganism sufficient to reduce the level of said sugar component; preferably further adding to the solution an amount of activated carbon sufficient to assist the color component in the solution; and recovering the solution to yield a decolorized, low-sugar solution of MeG. Also disclosed is a process comprising the steps of providing a solution containing MeG and dextrose; and adding to the solution an amount of a sugar-converting microorganism sufficient to reduce the level of the dextrose in the solution. Decolorized MeG solutions prepared by the foregoing processes also are disclosed.
    Type: Grant
    Filed: October 27, 1998
    Date of Patent: January 25, 2000
    Assignee: Grain Processing Corporation
    Inventors: Donald L. Johnson, Steven L. Ramsden, Roger E. McPherson
  • Patent number: 5830715
    Abstract: Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
    Type: Grant
    Filed: October 30, 1997
    Date of Patent: November 3, 1998
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Michio Kubota, Keiji Tsusaki, Kazuko Hattori, Toshiyuki Sugimoto
  • Patent number: 5716813
    Abstract: Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
    Type: Grant
    Filed: February 26, 1996
    Date of Patent: February 10, 1998
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Michio Kubota, Keiji Tsusaki, Kazuko Hattori, Toshiyuki Sugimoto
  • Patent number: 5578339
    Abstract: A process for the preparation of a sweetener, in which sucrose is converted enzymatically into a saccharide mixture which is called "isomerized sucrose" and has a disaccharide content of more than 85% by weight, then non-isomerized remaining sucrose is removed from the latter by enzymatic and/or H.sup.+ ion-catalyzed cleavage, and this product is catalytically hydrogenated. Preferably either before or after the catalytic hydrogenation, the resulting mixture is subjected to a chromatographic separation. The sweeteners prepared by this process contain either a mixture of 10 to 50% by weight of 6-O-.alpha.-D-glucopyranosyl-D-sorbitol; 2 to 20% by weight of 1-O-.alpha.-D-glucopyranosyl-D-sorbitol; and 30 to 70% by weight of 1-O-.alpha.-D-glucopyranosyl-D-mannitol or of 5 to 10% by weight of 6-O-.alpha.-D-glucopyranosyl-D-sorbitol; 30 to 40% by weight of 1-O-.alpha.-D-glucopyranosyl-D-sorbitol; and 45 to 60% by weight of 1-O-.alpha.-D-glucopyranosyl-D-mannitol.
    Type: Grant
    Filed: May 5, 1994
    Date of Patent: November 26, 1996
    Assignee: Sudzucker Aktiengesellschaft Mannheim/Ochsenfurt
    Inventors: Markwart Kunz, Hanspeter Degelmann, Wolfgang Wach, Mohammad Munir, Jorg Kowalczyk, Manfred Vogel
  • Patent number: 5508183
    Abstract: A process for converting cellulosic materials, such as waste paper, into fuels and chemicals, such as sugars and ethanol, utilizing enzymatic hydrolysis of the major carbohydrate of paper: cellulose. A waste paper slurry is contacted by cellulase in an agitated hydrolyzer. An attritor and a cellobiase reactor are coupled to the agitated hydrolyzer to improve reaction efficiency. Additionally, microfiltration, ultrafiltration and reverse osmosis steps are included to further increase reaction efficiency. The resulting sugars are converted to a dilute product in a fluidized-bed bioreactor utilizing a biocatalyst, such as microorganisms. The dilute product is then concentrated and purified.
    Type: Grant
    Filed: May 9, 1994
    Date of Patent: April 16, 1996
    Assignee: Martin Marietta Energy Systems, Inc.
    Inventors: Timothy C. Scott, Charles D. Scott, Brendlyn D. Faison, Brian H. Davison, Jonathan Woodward
  • Patent number: 5506123
    Abstract: A method of processing waste is disclosed wherein the municipal solid waste is segregated and processed to recover reusable rubber, metal, plastic, glass and the remaining organic portion of the waste stream is used to make lactic acid and other chemicals. One process utilizes a pretreatment step with dilute sulfuric acid to reduce the heavy metal content of the cellulosic component of the municipal solid waste which may contaminate the produced lactic acid or inhibit the fermentation of the sugars obtained from such waste. In another, the heavy metal content of the cellulosic component of municipal solid waste is removed via an ionic exchange process, after hydrolysis with sulfuric acid. A process for an economical, energy efficient production of lactic acid from municipal solid waste is also disclosed.
    Type: Grant
    Filed: April 14, 1995
    Date of Patent: April 9, 1996
    Assignee: Controlled Environmental Systems Corporation
    Inventors: Rodger Chieffalo, George R. Lightsey
  • Patent number: 5441644
    Abstract: A method for isolating and purifying trehalose from trehalose-containing solutions is described. Using ultrafiltration and selective concentration, highly pure crystals of trehalose dihydrate are obtained from aqueous solution.
    Type: Grant
    Filed: February 2, 1994
    Date of Patent: August 15, 1995
    Assignee: Ajinomoto Co., Inc.
    Inventor: Naoyuki Kinouchi
  • Patent number: 5348871
    Abstract: A process for converting cellulosic materials, such as waste paper, into fuels and chemicals utilizing enzymatic hydrolysis of the major constituent of paper, cellulose. A waste paper slurry is contacted by cellulase in an agitated hydrolyzer. The cellulase is produced from a continuous, columnar, fluidized-bed bioreactor utilizing immobilized microorganisms. An attritor and a cellobiase reactor are coupled to the agitated hydrolyzer to improve reaction efficiency. The cellulase is recycled by an adsorption process. The resulting crude sugars are converted to dilute product in a fluidized-bed bioreactor utilizing microorganisms. The dilute product is concentrated and purified by utilizing distillation and/or a biparticle fluidized-bed bioreactor system.
    Type: Grant
    Filed: May 15, 1992
    Date of Patent: September 20, 1994
    Assignee: Martin Marietta Energy Systems, Inc.
    Inventors: Charles D. Scott, Brendlyn D. Faison, Brian H. Davison, Jonathan Woodward
  • Patent number: 5238826
    Abstract: Process for manufacture of D-xylose characterized by the fact that:in a first step, syrup of D-xylulose is subjected to an enzymatic isomerization in M.sub.3 providing a mixture of D-xylose and D-xylulose,in a second step, the abovesaid mixture is subjected to chromatographic treatment in M.sub.4 leading to at least two fractions of which one is highly enriched in D-xylose (fraction X.sub.1) et of which the other is highly enriched in D-xylulose (fraction X.sub.2),in a third step, the fraction X.sub.2 is recycled through a pipe P to M.sub.3,the D-xylose being recovered from the fraction X.sub.1, the latter can also be subjected directly to a hydrogenation step.
    Type: Grant
    Filed: June 14, 1990
    Date of Patent: August 24, 1993
    Assignee: Roquette Freres
    Inventors: Jean-Bernard Leleu, Pierrick Duflot, Jean-Jacques Caboche
  • Patent number: 4950608
    Abstract: A temperature regulating container with a heater and a metal block in which test tubes with test samples are therein inserted and kept isothermally provides a plurality of heat pipes embedded in the metal block and extended to the heater section located at bottom of the metal block uniformally maintain the temperature in the metal block. Heat tubes are further extended downwardly to a cooling chamber provided at the bottom of heater such that when a cooling medium, water or air flows in the cooling chamber, the metal block is cooled respondingly provide accurate cooling of test samples in test tubes according to a desired program.
    Type: Grant
    Filed: April 25, 1989
    Date of Patent: August 21, 1990
    Assignee: Scinics Co., Ltd.
    Inventor: Masaru Kishimoto
  • Patent number: 4920052
    Abstract: A glucose isomerase useful for the conversion of glucose to fructose can be prepared by growing under aerobic conditions a culture of a species of Microbacterium in a medium containing appropriate nutrients and then recovering the enzyme.
    Type: Grant
    Filed: February 5, 1988
    Date of Patent: April 24, 1990
    Assignee: Miles Inc.
    Inventor: Graham S. Byng
  • Patent number: 4770784
    Abstract: A scrubbing process for high feed concentrations of different materials in liquid streams.
    Type: Grant
    Filed: November 29, 1985
    Date of Patent: September 13, 1988
    Assignee: The Standard Oil Company
    Inventors: James C. Davis, Michael F. McGuiggan, Terry Berrett
  • Patent number: 4753885
    Abstract: Foam is controlled in the sugar industry and yeast industry by a process in which oxyalkylation products of the formula IR--O--(X.sub.1).sub.n --(X.sub.2).sub.m --(X.sub.3).sub.p --Zwhere R is alkyl of 6 to 22 carbon atoms or alkylphenyl where alkyl is of 6 to 12 carbon atoms, X.sub.1 and X.sub.3 are ethylene oxide units, n and p are each from 0 to 15 and the sum of n and p is not less than 2, the groups X.sub.2 are propylene oxide and/or butylene oxide units, m is from 0 to 15 and Z is straight-chain or branched alkyl of 1 to 4 carbon atoms, allyl or benzyl, having a turbidity point of <75.degree. C., are used as antifoams.
    Type: Grant
    Filed: July 23, 1987
    Date of Patent: June 28, 1988
    Assignee: BASF Aktiengesellschaft
    Inventors: Wolfram Dietsche, Klaus Lorenz, Christos Vamvakaris, Albert Hettche
  • Patent number: 4748117
    Abstract: A new method is provided for forming crystals of amphotericin B directly in fermentation broth which crystals are easily separable without use of costly solvent extraction techniques which method includes a direct microbial cell autolysis step to induce crystal formation.
    Type: Grant
    Filed: April 16, 1986
    Date of Patent: May 31, 1988
    Assignee: E. R. Squibb & Sons, Inc.
    Inventors: Raphael Y. Ko, Laszlo J. Szarka
  • Patent number: 4650757
    Abstract: Enzymatic conversion of polysaccharides to monosaccharides or lower molecular weight polysaccharides is carried out by means of an enzyme derived from B. megaterium which exhibits alpha-amylase activity.
    Type: Grant
    Filed: May 23, 1985
    Date of Patent: March 17, 1987
    Assignee: CPC International Inc.
    Inventors: Marie-Henriette David, Horst Gunther, Jean-Claude de Troostembergh
  • Patent number: 4532232
    Abstract: A separation agent composed of a water insoluble solid substance with a convatently bound lectin. Said solid substance is structurally composed of a water insoluble silica or silicate material as basic material and via a linkage to silicon atoms in the silica or silicate material, a covatently bound lectin.
    Type: Grant
    Filed: December 23, 1983
    Date of Patent: July 30, 1985
    Assignee: Pernovo AB
    Inventors: Per-Olof Larsson, Klaus Mosbach, Axel Borchert
  • Patent number: 4530902
    Abstract: A ferric salt of the fructose-1,6-diphosphate is disclosed, which can be precipitated quantitatively from FDP-containing mixtures and has the formula (FDP).sub.3 Fe.sub.4. For preparing such salt, a ferric salt and an alkali are added to a fermentation broth which contains FDP, whereafter the precipitate is thoroughly water washed to remove the alkali metal salt and the washed solids are dried or frozen. The ferric salt has many advantages over the calcium salt used heretofore and no organic solvents are any more required.
    Type: Grant
    Filed: September 28, 1979
    Date of Patent: July 23, 1985
    Assignee: Biomedica Foscama Industria Chimico Farmaceutica S.p.A.
    Inventors: Giulio C. Perri, Tommaso Bianco, Beniamino Piccirilli
  • Patent number: 4450238
    Abstract: The invention relates to a process for the production of the alpha-galactosidase enzyme by culturing yeasts of the Saccharomyces cerevisiae genus in a temperature range from 20.degree. C. to 40.degree. C.The invention also relates to a process for enzymic hydrolysis of raffinose by alpha-galactosidase from Saccharomyces cerevisiae. Such hydrolysis may take place with the yeast cells being present or also in the presence of enzymic extracts, both as such and enriched. An important advantage of the invention is the high alpha-galactosidasic activity of the selected microorganisms, together with the absence of any invertasic activity.
    Type: Grant
    Filed: November 5, 1982
    Date of Patent: May 22, 1984
    Assignee: E. N. I. Ente Nazionale Idrocarburi
    Inventors: Vincenza Vitobello, Paolo Branduzzi, Nadia Cimini
  • Patent number: 4431737
    Abstract: The enzyme alpha-galactosidase is prepared from a constitutive mutant of the strain Saccharomyces cerevisiae NRRL-Y-12057, said mutant being marked by the identification number NRRL-Y-12533.
    Type: Grant
    Filed: November 30, 1982
    Date of Patent: February 14, 1984
    Assignee: Anic, S.p.A.
    Inventors: Roberto Olivieri, Paolo Pansolli, Eugenio Fascetti, Pierluigi Ciuffolotti
  • Patent number: 4421852
    Abstract: High fructose syrups are obtained from Jerusalem artichoke tubers and other naturally-occurring inulin-containing materials by extraction of the inulin and any related fructans from the tubers with water, elimination of some low molecular weight nitrogenous species and minerals from the aqueous extract by ultrafiltration, enzymatic hydrolysis of the inulin to fructose and glucose, separation of the reducing sugars from higher molecular species by ultrafiltration, and evaporative concentration of the purified reducing sugars solution to a syrup. The processing also removes colorants initially present in the aqueous extract. The dissolved solids in the syrup comprise at least 90 wt % reducing sugars and these sugars are constituted at least 60 wt %, often at least 75 wt %, by fructose with the balance glucose.
    Type: Grant
    Filed: August 9, 1982
    Date of Patent: December 20, 1983
    Inventors: Ernst Hoehn, Curtis J. McKay, E. Donald Murray
  • Patent number: 4376167
    Abstract: The invention relates to a process for the production of the alpha-galactoxidase enzyme by culturing yeasts of the Saccharomyces cerevisiae genus in a temperature range from 20.degree. C. to 40.degree. C.The invention also relates to a process for enzymic hydrolysis of raffinose by alpha-galactoxidase from Saccharomyces cerevisiae. Such hydrolysis may take place with the yeast cells being present or also in the presence of enzymic extracts, both as such and enriched. An important advantage of the invention is the high alpha-galactoxidasic activity of the selected microorganisms, together with the absence of any invertasic activity.
    Type: Grant
    Filed: December 5, 1980
    Date of Patent: March 8, 1983
    Assignee: E.N.I. Ente Nazionale Idrocarburi
    Inventors: Vincenza Vitobello, Paolo Branduzzi, Nadia Cimini
  • Patent number: 4327183
    Abstract: This invention provides a method for purifying crude fatty acid esters of saccharide containing at least fatty acid glycerides as impurity, and which method comprises decomposing the fatty acid glycerides by the treatment thereof with a lipid splitting enzyme or with a combination of a lipid splitting enzyme and a reducing agent in the presence of water.
    Type: Grant
    Filed: December 5, 1980
    Date of Patent: April 27, 1982
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Takayoshi Masuda, Masaru Honjo, Tsutomu Takase, Yoshimoto Watanabe
  • Patent number: 4288551
    Abstract: A process for removing color, turbidity, flavor, and odor from impure sugar syrups by entrapping the sugar impurities in an insoluble, primary calcium phosphate or aluminum hydroxide floc at about neutral pH, adding a suitable amount of hydrogen peroxide, rapidly decomposing the hydrogen peroxide with catalase to form a quantity of oxygen bubbles, and, during bubble formation, adding a polyelectrolyte to convert the primary floc into a secondary floc in which the oxygen bubbles are entrapped thereby causing flotation. The purified sugar syrup is then filtered with or without activated carbon and small amounts of a filter aid to produce a sugar syrup with substantially reduced color, turbidity, flavor, and odor.
    Type: Grant
    Filed: March 10, 1980
    Date of Patent: September 8, 1981
    Assignee: The Coca-Cola Company
    Inventors: Geir V. Gudnason, Joseph E. Stell
  • Patent number: 4277563
    Abstract: Fructose in a purified form is recovered from plant parts containing fructose polymer, such as inulin, by treatment of the mixed plant parts with an aqueous solution of fructose polymer hydrolyzing enzyme, such as inulase, followed by recovering the remaining solid plant parts and recovering fructose crystals from the aqueous solution.
    Type: Grant
    Filed: November 19, 1979
    Date of Patent: July 7, 1981
    Assignee: Stamicarbon, B.V.
    Inventor: Pieter L. Kerkhoffs