Abstract: A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes an antimicrobial treatment mechanism to inactivate microbes flushed from the biological sample by preservation fluid flowed therethrough. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature while simultaneously treating microbial infections to prevent transmission between a donor and a recipient.

Abstract: A Descemet Membrane Endothelial Keratoplasty (DMEK) holder for placement within a vial for supporting a corneal tissue inserter is provided. The DMEK holder includes a top and an opposite bottom, at least one support member extending between the top and the bottom, with a bottom portion of the at least one support member sized and shaped to fit against a bottom inner surface of the vial to thereby support the corneal tissue inserter within the vial, and a first annular member having an opening sized and shaped such that a bottom end of the corneal tissue inserter is insertable through the opening and the first annular member separates the corneal tissue inserter from an inner surface of the vial and positions the corneal tissue inserter within the vial away from inner surfaces of the vial to thereby improve retrieval of the corneal tissue inserter by a surgeon.

Abstract: A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and/or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and/or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.

Abstract: A sensor is attached to a cooling and warming box accommodating a cooling agent. A server device manages a transportation company that requests transportation of the cooling and warming box. The server device wirelessly receives box information indicating a detection result from the sensor. The server device receives replacement information of the cooling and warming agent accommodated in the cooling and warming box from a freezing center. The server device sends the box information and the replacement information received wirelessly to the managed transportation company.

Abstract: Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially an organ such as a lung, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. Gas passing into and out of the organ may be conditioned to prolong tissue viability.

Abstract: The invention provides, in various embodiments, a method comprising placing an ex-vivo heart in a chamber of an organ care system; connecting the ex-vivo heart to a perfusion fluid circuit of the organ care system; starting a pump configured to provide, via the perfusion fluid circuit, a perfusion fluid to the ex-vivo heart; synchronizing at least one maximum output of the pump with an r-wave of the ex-vivo heart; testing the ex-vivo heart or the organ care system; adjusting a setting of the organ care system in response to a result of the testing; and after adjusting the setting of the organ care system in response to the result of the testing, re-testing the ex-vivo heart or the organ care system.

Abstract: Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.

Abstract: A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

Abstract: Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially an organ such as a lung, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. Gas passing into and out of the organ may be conditioned to prolong tissue viability.

Abstract: The present disclosure includes a system to preserve tissue and associated methods. The system can include a portable oxygen source to provide oxygen and apply a pressure gradient to a flow of the oxygen, an organ preservation system having an inlet to fluidly couple to vasculature of the tissue, and an oxygen line to fluidly couple the portable oxygen source to the organ preservation system at the inlet, wherein the portable oxygen source is to apply the pressure gradient to pump fluid containing the oxygen through the organ preservation system.

Abstract: A device for placing a lung in a variety of different inflation states using positive air pressure. An exemplary device includes a housing and an air supply component. The housing includes a platform receives at least one of a synthetic lung or a real lung. The platform is at the same air pressure as a surrounding environment. The air supply component is located within the one or more internal cavities of the housing. The air supply component inflates the synthetic lung or the real lung with positive pressure.

Abstract: Systems and methods to perfuse isolated tissue are provided to avoid ischemia-related tissue damage over an extended perfusion time is provided. A system can comprise a reservoir sized and dimensioned to contain a perfusate. A perfusion supply line can comprise a pump, a cooling system, an oxygenator, and at least one filter. The perfusion supply line can be in fluid communication with the reservoir to draw the perfusate from the reservoir and cool and oxygenate the perfusate. The system can also include an arterial line in fluid communication with the perfusion supply line to direct perfusate from the reservoir to the isolated tissue. A venous outflow can be in fluid communication with the isolated tissue to remove the perfusate from the isolated tissue. A perfusion return line can be in fluid communication with the venous outflow to return the perfusate from the isolated tissue to the reservoir.

Abstract: An organ preservation system includes an organ container, an organ holder, a liquid supply tube, a drainage tube, and a pressure regulator. The organ container has a closed organ preservation space. The organ holder holds an organ in the organ preservation space. The liquid supply tube supplies a liquid to the organ. The drainage tube drains the liquid from the organ. The pressure regulator regulates air pressure in the organ preservation space. The organ container includes first to third connectors providing communication between the organ preservation space and an exterior space. The first, second, and third connectors are detachably connected to a liquid supply tube, a drainage tube, and a suction tube, respectively. When the organ is preserved ex vivo while being perfused with the liquid, the organ can be connected with ease to the tubes, and this ensures hermetical sealing of the space where the organ is preserved.

Abstract: The present invention relates to a device for holding human or animal corneal tissue previously removed for photon treatment thereof with electromagnetic radiation, notable in that the holding device comprises: a stack of elements along a longitudinal axis (A-A?) of the device, said stack comprising: a first plate (1) that is transparent to the electromagnetic radiation, a peripheral seal (4) positioned on the first plate (1), the peripheral seal (4) being intended to extend around the corneal tissue (6), a second plate (2) that is transparent to the electromagnetic radiation on the peripheral seal (4), an immobilizing system (51, 52) for immobilizing the stack of elements and able to press the first and second plates (1, 2) firmly against the corneal tissue in order to apply mechanical stress to the anterior and posterior faces of the corneal tissue.

Abstract: A portable, ex vivo perfusion system for preserving detached biological tissue includes a receptacle for housing the tissue in a normothermic environment, a perfusion core to pump perfusate through the tissue via at least one conduit, at least one detection device to measure parameters during perfusion, and at least one parameter control device to maintain the parameter in a predetermined threshold. The system also include a controller with instructions to receive the measured parameters, compare the parameters to predetermined thresholds, and when the parameters are outside the thresholds change an output of the at least one parameter control device to get the parameters within the threshold and alert a user that parameters were outside the thresholds.

Abstract: Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

Abstract: A system and method for automated and closed cell culture system. The system includes a disposable assembly, actuators, sensors, software/firmware and smart device App. The disposable includes fresh media, drug or reagents containers, waste container, sample out containers, cell culture flask, well, bag or bioreactor and active or passive pumping elements. The system is expanded to multiple containers cell or organ processing. In all these precisely controlling the fluids are achieved by interdigitated differential capacitance measurements. The system has provision for imaging, machine vision control, controlled over the internet, volume metering and can use wide variety of pumps for actuation. The system is programmed to perform cGMP protocols and operated as a portable instrument.

Abstract: Embodiments of the present disclosure are directed to systems, apparatuses, devices and methods for tissue cultivating. Such systems may comprise a plurality of bioreactors, each bioreactor comprising a bag having a mandrel tube arranged therein, and a fluid management system for managing fluid flow among the plurality of bioreactors. The fluid management system can include at least one intra-luminal pump, configured to flow intra-luminal fluid in a first direction in the bioreactors, at least one media pump, configured to flow biomedia fluid in the bioreactors in a second direction opposite to the first direction, and a plurality of valves and/or clamps to effect at least one of filling, flowing, cessation of flow, and draining of at least one of the intra-luminal fluid and biomedia fluid tubes among the bioreactors.

Abstract: The present disclosure provides methods for forming stable three-dimensional vascular structures, such as blood vessels and uses thereof. More specifically, the present disclosure provides methods for culturing differentiated endothelial cells that include an exogenous nucleic acid encoding ETV2 transcription factor on a matrix under conditions that express exogenous ETV2 protein in the endothelial cell to form stable three-dimensional artificial blood vessels without the use of a scaffold, pericytes or perfusion. The present disclosure also provides stable three-dimensional blood vessels that are capable of autonomously forming a functional three-dimensional vascular network, and uses thereof.

Abstract: A personal laboratory apparatus includes a plurality of life sciences laboratory modules disposed on or in a common housing. The laboratory modules include a cooling station, a heating station, an incubator, a bioreactor, and a removable fluidic cartridge that is fluidly coupled to the bioreactor. The personal laboratory apparatus can be sold as a kit with a wetware kit that includes laboratory hardware and ingredients for performing a genetic-engineering experiment. The wetware kit includes a plurality of test tubes and a pre-measured volume or mass of a plurality of materials, each pre-measured volume or mass disposed in a corresponding test tube.

Abstract: This document describes systems and methods for integrated mechanical loading of tissue. The system includes a three-dimensional tissue comprising organic material. The system includes a strip of bendable material. The strip includes a first region proximate to a first end of the strip coupled to the tissue. The strip includes a second region near a second end of the strip for coupled to the tissue, the second end being opposite the first end, wherein the tissue exerts a force on the strip to bend the strip, the force caused by contraction of the tissue, and wherein the strip exerts a stress on the tissue.

Abstract: The present invention relates to microfluidic or millifluidic chips (1) comprising at least one pressure sensing unit (4) able to measure a fluid flow pressure. The present invention also relates to a method for a direct and contact-free measuring of a local pressure of a fluid circulating in a microfluidic circuit, using a microfluidic or millifluidic chips (1) according to the invention.

Abstract: The present invention relates to organ perfusion systems that can be used at room temperature. The organ perfusion systems do not comprise a temperature controller. In some embodiments, the organ perfusion systems do not comprise a cleaning device for cleaning the perfusion fluid. The perfusion fluid can comprise Williams' medium E. The organ perfusion systems can be portable and can be used to preserving an organ, preventing ischemic damage in an organ, or recovering an ischemically damaged organ.

Abstract: Disclosed is a device for the perfusion of an organ, including: a container of fluid, containing an organ bathed in the perfusion fluid; a first path including an inlet, an outlet and a pump; and a second path including an inlet, an outlet and a pump. The “arterial” outlet of the first path has a diameter smaller than a diameter of the “portal” outlet of the second path. The device additionally includes, between the pump and the outlet of the first path and/or between the pump and the outlet of the second path, an oxygenation unit arranged to oxygenate the fluid emerging from the “arterial” outlet of the first path more than the fluid emerging from the “portal” outlet of the second path. The device can include a communication path between the first path and the second path in order to oxygenate the second path. Use in liver transplantation.

Abstract: Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs. The bioreactor comprises: an organ chamber: an ingres line connecting the organ chamber and a reservoir system and comprising an arterial line, a venous line and a tracheal line; an egress line connecting the chamber and the reservoir system, pumps in ingress and egress lines; a controller to control fluid exchange; a chamber pressure sensor connected to the organ chamber.

Abstract: An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.

Abstract: A minimally invasive system using a surgical robot as a three-dimensional printer for fabrication of biological tissues inside the body of a subject. A preoperative plan is used to direct and control both the motion of the robot and the robotic bio-ink extrusion. The robotic motion is coordinated with the ink extrusion to form layers having the desired thickness and dimensions, and use of different types of ink enables composite elements to be laid down. Such systems have a small diameter bio-ink ejecting mechanism, generally in the form of a piston driven cannula, enabling access to regions such as joints, with limited space. The robotic control is programmed such that angular motion takes place around a pivot point at the point of insertion into the subject. The bio-inks can be stored in predetermined layers in the cannula to enable sequential dispensing from one cannula.

Abstract: A novel macroscale, contactless, label-free method to print in situ three-dimensional (3D) particle assemblies of different morphologies and sizes is demonstrated using non-adherent (blood) and adherent (MCF-7 and HUVEC) cells. This method of manipulating particles such as cells or biological moleules does not necessarily require the use of nozzles that can contaminate the cell suspension, or to which cells can adhere. Instead, the intrinsic diamagnetic properties of particles such as cells are used to magnetically manipulate them in situ in a nontoxic paramagnetic medium, creating various shapes such as (a) rectangular bar, (b) three-pointed star, and (c) spheroids of varying sizes. A normal distribution of 3D cell structures is produced when formed through magnetic assembly. The use of this method in co-culturing of different cell lines is also demonstrated.

Abstract: Blood and platelet storage and rejuvenating compositions that include triciribine, triciribine metabolites, triciribine analogs, and mixtures of the same are disclosed. Such compositions can be useful in methods for treating (e.g., storing and rejuvenating) red blood cells and platelets.

Abstract: An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue; an oxygenator for oxygenating perfusate that circulates through the perfusion circuit; and an oxygen supply device such as an oxygen concentrator or an oxygen generator configured to supply oxygen to the oxygenator. A method of perfusing an organ or tissue includes producing oxygen from a device such as an oxygen concentrator and an oxygen generator; supplying the produced oxygen, preferably as the oxygen is produced, to a perfusate to oxygenate the perfusate; and perfusing the organ or tissue with the oxygenated perfusate. The produced oxygen preferably has a concentration greater than the oxygen concentration in air.

Abstract: Disclosed is a device for supporting and connecting an excised organ (such as a heart, a pair of lungs, a kidney, or a liver) during ex vivo perfusion. The device includes a resilient and flexible sheet having a first portion for contacting and supporting the organ thereon, and a second portion comprising an opening for forming a connection between the organ and a conduit to allow fluid communication between the conduit and the organ. The device also includes a magnetic material embedded in the second portion of the sheet for magnetically securing the connection between the conduit and the organ.

Abstract: Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially lung tissue, through the use of an expandable accumulator to maintain a constant pressure within the lumen of the organ even during external pressure fluctuations due to, for example, flight. Systems and methods may include prolonging donor organ viability in storage through the use of an organ container that mimics the geometry and orientation of the organ in vivo.

Abstract: Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially an organ such as a lung, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. Gas passing into and out of the organ may be conditioned to prolong tissue viability.

Abstract: The present invention discloses an afterload device for ex situ heart perfusion, which includes an afterload energy storage module, a pressure regulation module and two flow rate regulators that are connected through a conduit. The afterload energy storage module includes a rigid closed container and a flexible container. The perfusion fluid flows into the flexible container from one side thereof through the conduit, and the perfusion fluid flows out from the flexible container at another side thereof as the flexible container is subjected to the action of the medium pressure in the rigid hermetic container. The pressure regulation module includes two pressure regulation valves and a compressible-medium source, for regulating the pressure range within the rigid hermetic container to be always maintained between the set diastolic pressure and the set systolic pressure.

Abstract: Disclosed is a device for the perfusion of an organ, including: a container of fluid, containing an organ bathed in the perfusion fluid; a first path including an inlet, an outlet and a pump; and a second path including an inlet, an outlet and a pump. The “arterial” outlet of the first path has a diameter smaller than a diameter of the “portal” outlet of the second path. The device additionally includes, between the pump and the outlet of the first path and/or between the pump and the outlet of the second path, an oxygenation unit arranged to oxygenate the fluid emerging from the “arterial” outlet of the first path more than the fluid emerging from the “portal” outlet of the second path. The device can include a communication path between the first path and the second path in order to oxygenate the second path. Use in liver transplantation.

Abstract: Devices that may couple two or more apparatuses, such as an organ-on-a-chip device and a microfluidic device. Devices that include an organ-on-a-chip device, a microfluidic device, and a cap that couples the organ-on-a-chip device and the microfluidic device. Systems that include the devices and a detection unit. Methods for quantitation of insulin.

Abstract: This fluid handling device has a rotary member that is rotatable around the central axis. In the rotary member, a first protruding part for pressing and closing a valve of a flow channel chip and a recessed part for opening the valve without pressing the valve are disposed on the circumference of a first circle around the central axis. The rotary member further has a second protruding part for, when the recessed part is located at the valve in a state where the rotary member is rotated, pressing the valve so as not to open the valve.

Abstract: Disclosed is an ischaemia-free organ perfusion device and perfusion method. The perfusion device perfuses an isolated organ at normal temperature during whole transplantation, and comprises a first container, a second container, a first flow path, a second flow path, a third flow path and a fourth flow path. The perfusion device and the perfusion method are capable of maintaining the blood flow of the isolated organ during whole transplantation without interruption by machine perfusion, recovering the blood of the organ, effectively avoiding the interruption of blood supply for the organ during the whole transplantation, and improving the prognosis of organ transplantation.

Abstract: A multi-layered microfluidic system featuring tissue chambers for cells in a first layer and a plurality of medium channels for culture medium in a second layer. The tissue chambers fluidly connect to the medium channels such that media flows from the medium channels to the tissue chambers, forming large-scale perfused capillary networks. The capillary networks can undergo angiogenesis and vertical anastomosis. The multi-layered configuration of the system of the present invention allows for flexibility in design.

Abstract: The present disclosure provides an encapsulated liver tissue that can be used in vivo to improve liver functions, in vitro to determine the hepatic metabolism and/or hepatotoxicity of an agent and ex vivo to remove toxic compounds from patients' biological fluid. The encapsulated liver tissue comprises at least one liver organoid at least partially covered with a biocompatible cross-linked polymer. Processes for making the encapsulated liver tissue are also provided.

Abstract: Methods for ex vivo perfusion of organs (and/or tissues) with a perfusate designed to condition the organ with the desired effect being that upon transplant, said organ, having been administered said perfusate, is less likely to experience delayed graft function, deleterious effects of ischemia/reperfusion injury, including inflammatory reactions, and/or other detrimental responses that can injure the organ or recipient including precipitating or enhancing an immunological reaction from the recipient with the potential of compromising the graft's and/or recipients short teen and/or long term health and proper functionality while monitoring, sustaining and/or restoring the viability of the organ and preserving the organ for storage and/or transport.

Abstract: A bioreactor including a housing, a first fluid dispenser and a second fluid dispenser. The bioreactor is configured to receive a scaffold mounted within the housing with the first and second fluid dispensers being positioned to apply respective first and second fluids to at least two different regions of a mounted scaffold.

Abstract: An organ container includes flexible films and a tube holder. The films hold an organ in contact with the surface of the organ. The tube holder holds tubes that extend between the organ in the films and an outside of the films. This makes it possible to hold and preserve the organ while allowing a liquid to be perfused through the organ via the tubes. It is also possible to reduce movements of the organ relative to the organ container and to reduce damage to the organ.

Abstract: A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.

Abstract: A device for support of an organ ex vivo comprises a chamber with a first engagement feature. A support structure includes a second engagement feature. The first and second engagement features may be engaged so that the chamber body is carried by the support structure for rotation. The chamber body comprises first and second chamber components. In a first orientation of the chamber body, the first chamber component provides support for a first surface of the organ, and the second chamber component is removable so that a second surface of the organ is exposed to manipulation from outside the chamber body. In a second orientation of the chamber body, the second chamber component provides support for the second surface of the organ, and the first chamber component is removable so that the first surface of the organ is exposed to manipulation from outside the chamber body.

Abstract: A transport refrigeration unit and a method for controlling the transport refrigeration unit, wherein the transport refrigeration unit has a controller device for operating a compressor of the transport refrigeration unit with a continuously-variable speed, which allows the compressor to run continuously but with a smooth gradient range of speed variations. The controller device allows the transport refrigeration unit to have a start-stop operation stage, continuous run operation stage, and/or a cycle-sentry operation stage.

Abstract: The present disclosure relates to a method and apparatus for in-situ calibration and/or validation of a thermometer having a temperature sensor and a reference element composed at least partially of a material that undergoes a phase transformation at a phase transformation temperature, wherein the material remains in the solid phase in the phase transformation, the method including detecting and/or registering a measured value from the temperature sensor; detecting and/or registering a reference variable of the reference element; detecting the occurrence of the phase transformation based on a change of the reference variable; ascertaining a phase transformation time at which the phase transformation occurs; determining a sensor temperature using the temperature sensor at a measurement time that has the shortest time separation from the phase transformation time; and comparing the sensor temperature with the phase transformation temperature and/or determining a difference between the sensor temperature and the

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A bioreactor system may include (a) two or more fluidically coupled vessels configured to collectively carry out a bioreaction; (b) one or more fluidic paths coupling the two or more vessels to one another, where the fluidic paths are configured to provide flow of culture medium between the two or more vessels; (c) one or more fluid transfer devices along at least some of the one or more fluidic paths; and (d) a control system. The control system may be configured to (i) read or receive values of one or more parameters characterizing the culture medium in one or more of the vessels, and (ii) use the values to determine an adjusted flow rate in at least one of the fluidic paths to maintain substantially uniform values of the one or more parameters in the culture medium from vessel-to-vessel among the two or more vessels.

Abstract: A cell culture module, a cell culture system and a cell culture method are provided. The cell culture module includes a casing, a first fixer, a second fixer and a sheet-shaped carrier member. The casing has a chamber and at least one inlet/outlet. The inlet/outlet communicates with the chamber. The first fixer is fixed to the casing and located in the chamber. The second fixer is disposed in the chamber and is movable relative to the first fixer. The sheet-shaped carrier member is formed by arranging a plurality of cell culture carriers, and two opposite ends of the sheet-shaped carrier member are respectively fixed to the first fixer and the second fixer. The sheet-shaped carrier member is in an open state or a folded state according to a variation in a distance between the first fixer and the second fixer due to a movement of the second fixer.