Apparatus Patents (Class 435/283.1)
  • Patent number: 10723999
    Abstract: The present invention relates to a process for in vitro spermatogenesis from male germinal tissue comprising conducting maturation of testicular tissue comprising germ cells in a bioreactor which is made of a biomaterial and comprises at least one cavity wherein the germinal tissue is placed, and recovering elongated spermatids and/or spermatozoa.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: July 28, 2020
    Assignees: KALLISTEM, UNIVERSITÉ CLAUDE BERNARD LYON 1, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.), INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA), ECOLE NORMALE SUPERIEURE DE LYON
    Inventors: Marie-Hélène Perrard, Philippe Durand, Laurent David
  • Patent number: 10655107
    Abstract: Provided are a method capable of evaluating adherent cells under an environment similar to an in vivo environment by a culture method similar to a two-dimensional culture, and applications thereof. An adherent cell culture method uses, as a culture chamber (10), a chamber in which two or more culture spaces each having an equivalent diameter (D) that is 1 to 5 times the diameter of a desired spheroid and each having a height (H) that is 0.3 to 5 times the equivalent diameter are arranged and a surface of each of the culture spaces has a water contact angle of 45 degrees or less. Spheroids of adherent cells are cultured in the respective culture spaces (11) arranged in the culture chamber (10).
    Type: Grant
    Filed: September 20, 2012
    Date of Patent: May 19, 2020
    Assignee: Corning Incorporated
    Inventors: Yoko Ejiri, Satoru Ayano, Masaya Hosoda, Go Tazaki
  • Patent number: 10639597
    Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: May 5, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
  • Patent number: 10633625
    Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: April 28, 2020
    Assignee: Terumo BCT, Inc.
    Inventors: Brian J. Nankervis, Mark E. Jones
  • Patent number: 10625220
    Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: April 21, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
  • Patent number: 10620198
    Abstract: The invention concerns a cartridge for performing sample analysis in a portable point of care test device (POCT); a point of care assay device, comprising said cartridge and a reader for accommodating said cartridge, for detecting and/or quantifying an analyte in a sample; and a kit of parts comprising the said components of said point of care assay device.
    Type: Grant
    Filed: August 29, 2017
    Date of Patent: April 14, 2020
    Assignee: Invitron Limited
    Inventors: Andrew James Woodhead, James Stuart Woodhead
  • Patent number: 10597713
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Grant
    Filed: April 3, 2018
    Date of Patent: March 24, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Patent number: 10585091
    Abstract: Provided herein are methods, compositions, and devices for the identification and quantification of analytes, such as nucleic acids, proteins, cells or other biological samples. The methods, compositions, and devices are suited for accurate, portable analysis of small amounts of analyte.
    Type: Grant
    Filed: January 19, 2016
    Date of Patent: March 10, 2020
    Assignees: The University of Chicago, California Institute of Technology
    Inventors: Rustem F. Ismagilov, Stephanie McCalla, Travis Schlappi, Toan Huynh, Justin Rolando, Weishan Liu, Shencheng Ge, Jason E. Kreutz
  • Patent number: 10557165
    Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: February 11, 2020
    Assignee: KABUSHIKI KAISHA TOSHIBA
    Inventors: Koji Hashimoto, Keiko Ito, Mika Inada
  • Patent number: 10544450
    Abstract: The invention relates to a method for the duplication of nucleic acids by means of a polymerase chain reaction, in the case of which a cycle consisting of the steps of denaturing, annealing and elongation is repeatedly performed. In one embodiment, the yield (g) of specimens of a nucleic acid to be duplicated, at the end of at least one passage of the cycle, is less than 80 percent of the specimens of the nucleic acid present at the beginning of said passage and, in the case of at least one passage of the cycle, the reaction time (tA) is less than one second. In addition, in a further embodiment, the number (k) of passages of the cycle of the polymerase chain reaction is greater than 45 and/or in at least one of the passage the cycle time tc is less than 20 seconds.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: January 28, 2020
    Assignee: GNA Biosolutions GmbH
    Inventors: Federico Buersgens, Joachim Stehr, Lars Ullerich
  • Patent number: 10486156
    Abstract: Methods and systems for processing fluids utilizing a digital microfluidic device and transferring droplets from the digital microfluidic device to a downstream analyzer are described herein. Methods and systems in accordance with the present teachings can allow for the withdrawal of fluid from a digital microfluidic device, and can in some aspects enable the integration of a digital microfluidic device as a direct, in-line sample processing platform from which a droplet can be transferred to a downstream analyzer.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: November 26, 2019
    Assignees: DH Technologies Development Pte. Ltd., University of Toronto
    Inventors: John Lawrence Campbell, Kihwan Choi, Yves LeBlanc, Chang Liu, Aaron Wheeler
  • Patent number: 10464020
    Abstract: The present disclosure relates to a hydrogen peroxide vapor decomposition and detoxification system including a hydrogen peroxide vapor suction unit which sucks hydrogen peroxide vapor in a target space to be sterilized, a hydrogen peroxide vapor decomposition and detoxification unit which decomposes the sucked hydrogen peroxide vapor, a vapor discharge unit which discharges decomposition products of the hydrogen peroxide vapor, and a control unit which controls an amount of vapor suction of the vapor suction unit, decomposition and detoxification of the hydrogen peroxide vapor decomposition and detoxification unit, and the vapor discharge unit.
    Type: Grant
    Filed: January 25, 2019
    Date of Patent: November 5, 2019
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Kang Bong Lee, Yeong Cheon Kim, Yun Sik Nam, Jin Oh Jeon, Jong Ho Park
  • Patent number: 10448672
    Abstract: A vaporizer, including a removable panel; and a main body assembly such that the removable panel is removably attached to the main body assembly, wherein the main body assembly is further comprised of; a crucible/pan assembly such that a material to be vaporized is located within the crucible/pan assembly, an induction heater such that the induction heater is located adjacent to the crucible/pan assembly for induction heating of the crucible/pan assembly which in turn vaporizes the material to be vaporized located within the crucible/pan assembly, a removable battery assembly located adjacent to the induction heater, an electronics assembly operatively connected to the battery and the induction heater, and an interchangeable mouthpiece assembly operatively attached to the crucible/pan assembly for allowing vapors from the material to be vaporized to be inhaled by an end user.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: October 22, 2019
    Inventor: Richard Smayda
  • Patent number: 10434513
    Abstract: A method and microfluidic device are provided for containing a droplet having an outer surface at a predetermined location. The microfluidic device includes a plate having an upper surface and a central region communicating with the upper surface. The central region is adapted for receiving a droplet of fluid thereon. The central region includes an outer periphery that defines a first fluid constraint configured for discouraging fluid on the central region from flowing therepast. A second fluid constraint extends about the first fluid constraint. The second fluid constraint is configured for discouraging fluid flowing therepast. A third fluid constraint extends about the second fluid constraint. The third fluid constraint configured for discouraging fluid flowing therepast.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: October 8, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, David J. Guckenberger, Jr., Scott M. Berry, Hannah M. Pezzi
  • Patent number: 10415098
    Abstract: The present invention relates to nucleic acids and methods for detecting pathogens and beneficial microorganisms.
    Type: Grant
    Filed: November 5, 2014
    Date of Patent: September 17, 2019
    Inventors: Nicola Di Maiuta, Konrad Egli, Joachim Glaubitz, Simon Urwyler
  • Patent number: 10261077
    Abstract: A subtractive corrective assay device and methodology, whereby ail required binding and label detection reagents are initially located within the detection zone. Application of a magnetic field is used to selectively remove bound label from the detection zone by means of paramagnetic particles. The relationship between measured label concentration before and after the application of a magnetic field within the detection zone is used to accurately measure analyte concentration within the sample.
    Type: Grant
    Filed: May 16, 2016
    Date of Patent: April 16, 2019
    Assignee: LUMIRADX UK LTD
    Inventors: Phillip Lowe, Steven Alexander Keatch, Brian McGuigan
  • Patent number: 10252907
    Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
    Type: Grant
    Filed: February 22, 2017
    Date of Patent: April 9, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Keith J. Breinlinger, Daniele Malleo, Gaetan L. Mathieu, J. Tanner Nevill, Alexander H. Slocum, Mark P. White
  • Patent number: 10233505
    Abstract: The present invention is concerned with PCR-based detection methods and kits for the identification, differentiation, and quantification of different bacterial strains (e.g., Gram-negative bacterial strains), and also association of two or more PCR-positive genes to a single genome. The methods generally comprise carrying out PCR reactions using at least a first PCR primer set and/or probe for at least one target nucleic acid; and a second PCR primer set and/or probe for at least a second target nucleic acid. Positive PCR reaction products are then detected to determine test samples containing positive PCR reaction products for both the first and second target nucleic acids. This information can be used to calculate the gene association rate to determine whether the sample contains, for example, Shiga toxin-producing E. coli of the O-type serogroup.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: March 19, 2019
    Assignee: KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Jianfa Bai, Xuming Liu, Gary Anderson
  • Patent number: 10221440
    Abstract: The invention relates to medicine and to veterinary medicine, and specifically to means for determining the sensitivity of various microorganisms, including bacteria and fungi, to antimicrobial substances. The method for determining the sensitivity of microorganisms to antimicrobial substances comprises taking biological material, incubating microorganisms contained therein on a nutrient medium, introducing an antimicrobial substance to be investigated into the nutrient medium and subsequently assessing the result. A dense, rich nutrient medium is used for cultivating the microorganisms. The antimicrobial substance being investigated is introduced into the nutrient medium prior to cultivation of the microorganisms in a concentration close to the maximum concentration achievable at the location at which the biological material is taken. The sensitivity of the microorganisms to the antimicrobial substance is assessed after the appearance of visible growth of microorganisms in a control culture.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: March 5, 2019
    Inventors: Viktor Veniaminovich Tets, Georgy Viktorovich Tets
  • Patent number: 10220389
    Abstract: A microfluidic device comprises an inlet port for liquid, and a capillary channel in fluid connection to the inlet port for receiving liquid from the inlet port, the channel having a defined volume. At least one dissolvable valve is provided comprising a dissolvable membrane having a first side oriented towards the capillary channel, and a capillary connected to the second side of the dissolvable membrane such that when the membrane is dissolved by the liquid, liquid is transported through the valve to the second side of the membrane by capillary action. A method of controlling a flow of liquid uses such a microfluidic device.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: March 5, 2019
    Assignee: CAPITAINER AB
    Inventors: Göran Stemme, Gabriel Lenk, Olof Beck, Niclas Roxhed
  • Patent number: 10213967
    Abstract: A fabricating device of a three-dimensional (3-D) scaffold comprises a bubble generator, a bubble mixing channel, a coagulating solution channel and a bubble collector. The bubble generator includes a gas channel and a gel solution channel crossing the gas channel. The bubble mixing channel is connected with a first outlet of the bubble generator. The coagulating solution channel is connected with the bubble mixing channel. The bubble collector is connected with a second outlet of the bubble mixing channel.
    Type: Grant
    Filed: June 11, 2014
    Date of Patent: February 26, 2019
    Assignee: Academia Sinica
    Inventors: Keng-Hui Lin, Yi-Hsuan Lee
  • Patent number: 10161927
    Abstract: Among others, the present invention provides apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material.
    Type: Grant
    Filed: June 30, 2011
    Date of Patent: December 25, 2018
    Assignee: AnPac Bio-Medical Science Co., Ltd.
    Inventors: Chris C Yu, He Yu, Xuedong Du
  • Patent number: 10163629
    Abstract: Systems and methods for processing films on the surface of a substrate are described. The systems possess aerosol generators which form droplets from a condensed matter (liquid or solid) of one or more precursors. A carrier gas is flowed through the condensed matter and push the droplets toward a substrate placed in a substrate processing region. An inline pump connected with the aerosol generator can also be used to push the droplets towards the substrate. A direct current (DC) electric field is applied between two conducting plates configured to pass the droplets in-between. The size of the droplets is desirably reduced by application of the DC electric field. After passing through the DC electric field, the droplets pass into the substrate processing region and chemically react with the substrate to deposit or etch films.
    Type: Grant
    Filed: February 16, 2016
    Date of Patent: December 25, 2018
    Assignee: APPLIED MATERIALS, INC.
    Inventors: Ranga Rao Arnepalli, Nilesh Chimanrao Bagul, Prerna Sonthalia Goradia, Robert Jan Visser
  • Patent number: 10130945
    Abstract: Cell tubes that can be used both for pathology collection and subsequent cell processing include a tube with a cell bed at a lower portion of the tube. The tube can include a base member that can be detachable from the tube body. The tubes can be used to form cell (cytology) blocks that incorporate the cell bed. The cell bed can be an inert cell bed of paraffin.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: November 20, 2018
    Assignee: Wake Forest University Health Sciences
    Inventors: Shaozhou Ken Tian, Kim R. Geisinger
  • Patent number: 10101349
    Abstract: A test management system is presented. The system comprises an analyzer to perform tests a sample according to a first set of instructions, a manager module connected to the analyzer, and a first order interface connected to the manager module. The manager module directs activity of the analyzer according to a second set of instructions. The first order interface receives an order for a first analytical test and a second analytical test and transmits the order to the manager module. If the order is for the first analytical test, the test manager module forwards the order directly to the analyzer and the sample is analyzed by the analyzer according to the first set of instructions. If the order is for the second analytical test, the manager module handles the order according to the second set of instructions and generates and transmits secondary test requests to the analyzer.
    Type: Grant
    Filed: August 29, 2016
    Date of Patent: October 16, 2018
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Sascha Martin Antoni, Martin Burri, Werner Doeppen, Bernhard Eckert, Elke Faatz, Barbara Upmeier, Andreas Woeste, Dieter Roessler, Elena Pfaffenrot
  • Patent number: 10066203
    Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
    Type: Grant
    Filed: February 19, 2009
    Date of Patent: September 4, 2018
    Assignees: Janssen Biotech Inc., Nunc A/S
    Inventors: Benjamin Fryer, Shelley Nelson, Villy Nielsen, Tina Kristensen Marwood, Thomas Brevig
  • Patent number: 10068200
    Abstract: Embodiments of business systems and data processing systems and related methods, apparatus, compositions, systems, and articles of manufacture useful for providing mass spectrographic analysis services are disclosed. In embodiments, MS analysis services may be provided to customers without exposing proprietary information of the customer to the service provider or others.
    Type: Grant
    Filed: October 2, 2016
    Date of Patent: September 4, 2018
    Inventors: Matthew Paul Greving, Trent R. Northen
  • Patent number: 10040070
    Abstract: A reaction container 10 of the PCR device 1 has a tubular ring channel 20 formed by a substrate 11, a channel forming plate 12, and a cover plate 13. The ring channel 20 comprises an electrode pair 21 disposed, in a diametrical direction of an inner surface of one side wall of the ring channel 20, to face each other with a horizontal gap interposed therebetween, and is vertically erected. The gap length of the electrode pair 21 is across the entire width within a cross-section of the ring channel 20.
    Type: Grant
    Filed: January 21, 2016
    Date of Patent: August 7, 2018
    Assignee: Fluid Incorporated
    Inventor: Shuzo Hirahara
  • Patent number: 10023870
    Abstract: A DNA aptamer was obtained which has an affinity for His-tag, and contains a nucleotide sequence selected from SEQ ID No. 1 and SEQ ID No. 2, which has clear applications.
    Type: Grant
    Filed: May 17, 2014
    Date of Patent: July 17, 2018
    Assignee: UNIWERSYTET JAGIELLONSKI
    Inventors: Filip Bartnicki, Ewa Kowalska, Katarzyna Pels, Wojciech Strzalka
  • Patent number: 9938519
    Abstract: The present invention relates to methods and systems for the isolation of DNA on a microfluidic device and the subsequent analysis of the DNA on the microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for performing amplification reactions, such as PCR, and detection, such as thermal melt analysis, on the microfluidic.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: April 10, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventor: Michele R. Stone
  • Patent number: 9932634
    Abstract: Methods are provided for fast nucleic acid amplification in which concentrations of primers and polymerase are increased.
    Type: Grant
    Filed: May 23, 2013
    Date of Patent: April 3, 2018
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Steven Jared Farrar
  • Patent number: 9920351
    Abstract: A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.
    Type: Grant
    Filed: July 8, 2015
    Date of Patent: March 20, 2018
    Assignee: QUANTAMATRIX INC.
    Inventors: Sunghoon Kwon, Yong-Gyun Jung, Jung Il Choi, Hun Jong Na
  • Patent number: 9909172
    Abstract: Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (PCR). In one aspect, a method for cycling PCR includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first pH level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second pH level of the electrolytic fluid, in which the second pH level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands.
    Type: Grant
    Filed: August 1, 2016
    Date of Patent: March 6, 2018
    Assignee: The Regents of the University of California
    Inventors: Inanc Ortac, Sadik C. Esener
  • Patent number: 9897619
    Abstract: Disclosed is a method and apparatus for determining a concentration of a glycopeptide antibiotic containing a phenol moiety such as Vancomycin in a complex sample matrix by extracting the glycopeptide antibiotic from a metered portion of the complex sample matrix by exposing said metered portion to an extraction material having an affinity with the glycopeptide antibiotic; and exposing the extraction material to a metered portion of an eluent for releasing the glycopeptide antibiotic from the extraction material; and by determining a concentration of the glycopeptide antibiotic by adding a Gibbs reagent (2,6 dichloroquinone-4chloroimide) to the metered portion of the complex sample matrix or the eluent; activating the Gibbs reagent and, after the reaction between the activated Gibbs reagent and the antibiotic has stabilized; detecting the reaction product of the activated Gibbs reagent and the antibiotic in said eluent; and determining the concentration of the antibiotic in the complex sample matrix from the
    Type: Grant
    Filed: February 17, 2014
    Date of Patent: February 20, 2018
    Assignees: Sphere Medical Limited, UCL Business Plc
    Inventors: Natascha Kappeler, Rachel Anne McKendry, Russell Keay, David Pettigrew, Steven Andrew Fowler, Daren Joseph Caruana
  • Patent number: 9869612
    Abstract: The present invention provides a substrate collecting device that includes a first stage on which a sheet is placed with a plurality of substrates facing downward, an sampler which carries out a predetermined operation on some of the substrates, which are disposed at predetermined positions, from above the first stage thereby to cause the substrates to come off from the sheet and fall, an optical system and a collector disposed below the first stage, and a second stage, which integrally moves the optical system and the collector in a horizontal direction. The second stage is capable of positioning the optical system and the collector at two positions at which the optical system or the collector is disposed substantially vertically below a predetermined position.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: January 16, 2018
    Assignee: Olympus Corporation
    Inventor: Nobuhiko Morimoto
  • Patent number: 9835619
    Abstract: Acceleration and automation of immunohistochemical staining are achieved as follows: an automatic electric field immunohistochemical staining apparatus is configured to provided with a sample mounting unit, a solution supply unit, an electric field stifling unit, and a washing unit; the sample mounting unit on which a glass substrate with a tissue specimen fixed thereto is mounted and the electric field stirring unit that includes an upper electrode are operated in coordination to activate an antigen in the tissue specimen; the sample mounting unit and the solution supply unit containing various solutions are operated in coordination to supply a primary-antibody-containing solution to the tissue specimen; the sample mounting unit and the electric field stirring unit are operated in coordination to perform an antigen-antibody reaction of the antigen in the tissue specimen and a primary antibody; and the electric field stirring unit and the washing unit are operated in coordination.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: December 5, 2017
    Assignees: Governor of Akita Prefecture, Akita University
    Inventors: Yoichi Akagami, Masami Kagaya, Ryuta Nakamura, Hiroshi Ikeda, Yoshihiro Minamiya, Hiroshi Nanjo
  • Patent number: 9719123
    Abstract: Disclosed is a urine sample analyzer for analyzing particles contained in a urine sample and outputting analytical results. The analyzer includes a flow cell that accepts a measurement specimen, the measurement specimen comprising a urine sample mixed with a reagent, a light irradiation unit positioned to irradiate the flowing measurement specimen with light, a light detector that detects light from individual particles in the flowing measurement specimen, and a data processor that receives signal from the light detector, processes the signal to obtain parameter information corresponding to a length of a cell cluster, and classifies fungi in the measurement specimen into groups by using the parameter information.
    Type: Grant
    Filed: October 27, 2014
    Date of Patent: August 1, 2017
    Assignee: SYSMEX CORPORATION
    Inventors: Shota Tateyama, Masanori Kawano, Masatsugu Ozasa
  • Patent number: 9708600
    Abstract: Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analyzes of DNA).
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: July 18, 2017
    Assignee: DIOMICS CORPORATION
    Inventors: Jeff Morhet, Thomas Kindt, Franco Ferrari, Vasana Maneeratana, Frederic Zenhausern, Sean Grandfield, Beverly L. Wolgast
  • Patent number: 9669358
    Abstract: Methods and apparatus for cross flow process reactors are disclosed. A disclosed example includes a process chamber having an inlet and an outlet to allow a flow of fluid to flow therethrough and a plurality of trays disposed within the process chamber, and containing bulk material to treat the fluid. The example apparatus also includes a flow director to divide the flow into a plurality of partial flow streams, where each partial flow stream is to be directed towards at least one tray of the plurality of trays.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: June 6, 2017
    Assignee: Dürr Systems AG
    Inventors: Antoine Bose, Erhard Rieder
  • Patent number: 9662650
    Abstract: A fluidic cartridge for processing a liquid sample comprises a main channel for passing the liquid sample therethrough from an upstream end to a downstream end; and one or more branch channels that join the main channel for introducing liquid and gas into the main channel after the liquid sample has passed downstream of the one or more branch channels. The one or more branch channels including a first branch channel, wherein the first branch channel comprises: a gas inlet for introducing a gas into the first branch channel; a liquid inlet for introducing a liquid into the first branch channel; and a valve configured to move between a closed position in which it prevents liquid and gas in the first branch channel from passing into the main channel and an open position in which it permits liquid and gas in the first branch channel to pass into the main channel.
    Type: Grant
    Filed: July 28, 2014
    Date of Patent: May 30, 2017
    Assignee: Atlas Genetics Limited
    Inventors: Jay Kendall Taylor, Ben Arlett
  • Patent number: 9624540
    Abstract: Optical analytical devices and their methods of use are provided. The devices are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices include integrated illumination elements and optical waveguides for illumination of the optical reactions. The devices further provide for the efficient coupling of optical excitation energy from the waveguides to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices of the invention are well suited for miniaturization and high throughput.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: April 18, 2017
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Paul Lundquist, Stephen Turner
  • Patent number: 9617145
    Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: April 11, 2017
    Assignee: Berkeley Lights, Inc.
    Inventors: Keith J. Breinlinger, Daniele Malleo, Gaetan L. Mathieu, J. Tanner Nevill, Alexander H. Slocum, Mark P. White
  • Patent number: 9568425
    Abstract: Analytical nanostrips for clinical analysis are improved by using multifunctional coding (“multicoding”) to allow simultaneous identification of the particular assay, the value of the assayed analyte, and a calibration of the analyte. The multicoding layout on the nanostrip minimizes the number of zones that are required for a given assay. Moreover, the nanostrip can be scanned in real time during flow of the nanostrip through a detection beam. This both simplifies the assay and allows for alternative means of coding.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: February 14, 2017
    Assignee: DNA Medicine Institute, Inc.
    Inventors: Eugene Y. Chan, Moon Z. Chan
  • Patent number: 9557251
    Abstract: Cell tubes that can be used both for pathology collection and subsequent cell processing include a tube with a cell bed at a lower portion of the tube. The tube can include a base member that can be detachable from the tube body. The tubes can be used to form cell (cytology) blocks that incorporate the cell bed. The cell bed can be an inert cell bed of paraffin.
    Type: Grant
    Filed: June 25, 2013
    Date of Patent: January 31, 2017
    Assignee: Wake Forest University Health Sciences
    Inventors: Shaozhou Ken Tian, Kim R. Geisinger
  • Patent number: 9528149
    Abstract: A detection device includes a PCR processor for conducting a PCR process on a first drop to a fourth drop flowing in a flow channel, a boundary detector for detecting intensities of fluorescence outputted from the first drop to the fourth drop after the PCR process and acquiring boundaries between the first drop to the fourth drop flowing in a flow channel based on the intensities of fluorescence, and a detector for acquiring a number of the second drop and the fourth drop having an intensity of fluorescence greater than or equal to a first threshold based on the intensity of fluorescence and boundaries between the first drop to the fourth drop, and detecting whether or not the objective nucleic acid target includes at least one selected from the group consisting of a first nucleic acid target and a second nucleic acid target based on the number of the second drop and the fourth drop.
    Type: Grant
    Filed: February 20, 2016
    Date of Patent: December 27, 2016
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventor: Masahiko Tsukuda
  • Patent number: 9523123
    Abstract: Disclosed is a DNA detection device that detects a target DNA by detecting fluorescence output from sample droplets flowing through a flow path in a surface of a sensor chip, and that includes a fluorescence detector that detects fluorescence output from sample droplets flowing through the flow path, and a DNA detector that determines a type of fluorescent probe solution contained in each of the sample droplets based on a duration of the detected fluorescence and determines whether or not the sample droplet contains the target DNA based on whether intensity of the fluorescence is higher or lower than a threshold value.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: December 20, 2016
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventor: Masahiko Tsukuda
  • Patent number: 9498778
    Abstract: In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: November 22, 2016
    Assignee: GENMARK DIAGNOSTICS, INC.
    Inventors: Scott Corey, Brian Murphy, Eric Schneider, David Walter Wright, Dominic Aiello, Andrew Fish, Jay Srinivasan, George Maltezos
  • Patent number: 9494568
    Abstract: According to embodiments of the present invention, a passive counter flow free microfluidic device for sorting a sample of flagellated cells is provided.
    Type: Grant
    Filed: February 3, 2012
    Date of Patent: November 15, 2016
    Assignee: Neobios PTE LTD
    Inventors: Soon Chye Ng, Swee Lian Liow, Naiqing Chen, Yen Wah Tong, Saif Abdul Kadir Khan, Boon Sing Fang
  • Patent number: 9475053
    Abstract: A thermal cycler includes a holder that holds a biotip filled with a reaction mixture and liquid having a smaller specific gravity than the reaction mixture and being immiscible with the reaction mixture, the biotip including a channel in which the reaction mixture moves, a heating unit that heats a first portion of the channel when the biotip is in the holder, and a driving unit that disposes the holder and the heating unit by making a switch between a first disposition and a second disposition, the first disposition being such that the first portion is in a lowest part of the channel with respect to a gravitational force direction when the biotip is in the holder, the second disposition being such that a second portion that is a different portion from the first portion relative to a moving direction of the reaction mixture is in the lowest part of the channel with respect to the gravitational force direction when the biotip is in the holder.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: October 25, 2016
    Assignee: Seiko Epson Corporation
    Inventor: Hiroshi Koeda
  • Patent number: 9476809
    Abstract: Gassing or fumigation apparatus comprising a one-piece specimen receptacle block including at least two specimen receptacles formed therein and a fluid supply device formed therein for the at least two specimen receptacles, and a gassing or fumigation head for supplying gassing or fumigation medium, which gassing or fumigation head is connected to the specimen receptacle block, comprises a gassing or fumigation medium outlet for each of the at least two specimen receptacles and a device formed therein for conducting gassing medium to the gassing or fumigation medium outlets, and gassing or fumigation system including such a gassing or fumigation apparatus.
    Type: Grant
    Filed: February 13, 2008
    Date of Patent: October 25, 2016
    Assignee: PIETER VAN WEENEN & CO., GmbH
    Inventor: Tobias Krebs