Apparatus Patents (Class 435/283.1)
  • Patent number: 11779929
    Abstract: Embodiments of the invention include instruments and methods useful in preparing and delivering graft material to a surgical site. Some embodiments may particularly be directed to forming a graft from a blood clot and accurately and effectively handling and delivering the graft to a surgical site. Graft material may include blood components such as clotted fibrin derived from a patient's or a donor's blood.
    Type: Grant
    Filed: August 17, 2020
    Date of Patent: October 10, 2023
    Inventors: Jennifer Ng, Charles H. Brown, Jr., William R. Davis
  • Patent number: 11756196
    Abstract: A method for checking a dissection process in a laser microdissection system includes carrying out the dissection process for cutting out a dissectate from an object in a first region of the object by a laser beam. First image data is acquired of at least the first region of the object after the dissection process. It is examined whether the first image data has sharp structures within a region to be separated by the dissection process in order to determine whether the dissection process was successful.
    Type: Grant
    Filed: January 7, 2021
    Date of Patent: September 12, 2023
    Inventors: Falk Schlaudraff, Christoph Greb
  • Patent number: 11730152
    Abstract: The present embodiments of the invention generally relate to methods for sorting and imaging insects, including egg and larval life stages, useful for automated high throughput bioassays.
    Type: Grant
    Filed: November 3, 2020
    Date of Patent: August 22, 2023
    Inventors: Michael Freeman, Kathleen Marie Harding, Jingtong Hou, Bumshik Robert Pak, Phillip A Patten, Lindsey Della Wiemer, Takashi Yamamoto
  • Patent number: 11697789
    Abstract: The reactor is for manufacturing biogas from organic raw material using anaerobic digestion. The reactor includes a tubular reaction chamber with a substantially rectangular cross-section composed of a bottom, walls and a ceiling for processing raw material into end products. Agitation and transfer equipment are arranged in the reaction chamber and an external support frame structure is arranged on the outer surface included in the reaction chamber for stiffening and supporting the reaction chamber externally against forces generated by the raw material.
    Type: Grant
    Filed: November 23, 2018
    Date of Patent: July 11, 2023
    Inventor: Mika Rautiainen
  • Patent number: 11673137
    Abstract: A sample holder (10) comprises a sample chamber (33), a gas reservoir (32) and an upper layer (20) covering over the sample chamber (33) and gas reservoir (32), wherein a bottom surface of the upper layer (20) comprises a microstructure array (23) which overlies at least a portion of a top periphery of the sample chamber (33), and wherein the microstructure array (23) is in communication with a gas path which extends to the gas reservoir (32), to allow gas exchange between the sample chamber (33) and the gas reservoir (32).
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: June 13, 2023
    Assignee: Q-LINEA AB
    Inventors: Jonas Melin, Simon Uhrberg, Jonas Jarvius
  • Patent number: 11643681
    Abstract: Apparatus, systems, chips, and methods of performing a large number of simultaneous chemical reactions are provided herein. The chips of the invention comprise addressable units that can be addressed according to the temperature of the reaction to be run. The subject apparatus, systems, and chips are particularly suited for performing polymerase chain reactions on thousands of nucleic acid sequences, up to and including sequences of an entire genuine of an organism of interest.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: May 9, 2023
    Assignee: Takara Bio USA, Inc.
    Inventors: Victor Joseph, Amjad Huda, Alnoor Shivji
  • Patent number: 11633739
    Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.
    Type: Grant
    Filed: December 29, 2020
    Date of Patent: April 25, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kevin D. Ness, Donald A. Masquelier, Billy W. Colston, Jr., Benjamin J. Hindson
  • Patent number: 11629319
    Abstract: A method of vascularising a cell aggregate on a microfluidic device, microfluidic cell culture devices comprising perfusable vascular networks and kits and assays using the microfluidic cell culture devices are described. The microfluidic devices comprise one or more capillary pressure barriers allowing for formation of an extracellular matrix gel within a confined area of the network, in which cells can be cultured for different uses.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: April 18, 2023
    Assignee: Mimetas, B.V.
    Inventors: Paul Vulto, Sebastiaan Johannes Trietsch, Arnaud Nicolas
  • Patent number: 11590348
    Abstract: A system for controlling blood pressure includes a wearable interface having an internal contact surface, the wearable interface configured to at least partially encircle a first portion of a first limb of a subject, a sensing module carried by the wearable interface and configured to determine at least a change in blood pressure of the first limb of the subject, and an energy application module carried by the wearable interface and configured to apply energy of two or more types to the first limb of the subject.
    Type: Grant
    Filed: March 28, 2022
    Date of Patent: February 28, 2023
    Assignee: Adventus Ventures, LLC
    Inventors: Shahram Moaddeb, Faizal Abdeen, Rinda Sama
  • Patent number: 11591590
    Abstract: The present disclosure relates to the generation of an activated platelet product in which one or more of the presence or absence of clots, the timing of clot formation (if present), and/or the mechanical strength of clots (if present) is controlled by the presence or concentration of calcium ions during the activation process. In certain embodiments, the calcium ion concentration is controlled in the presence of pulsed electric fields or a chemical activator (e.g., thrombin) as part of the activation process.
    Type: Grant
    Filed: April 16, 2020
    Date of Patent: February 28, 2023
    Assignee: General Electric Company
    Inventors: Vasile Bogdan Neculaes, Andrew Soliz Torres, Steve Lambert Klopman
  • Patent number: 11517900
    Abstract: A method for separating and enriching sperm from a tissue sample comprises: obtaining a microfluidic separating system having an inlet end and an outlet end, and a membrane filter (e.g., hollow fiber membrane filter) fluidly connected to the outlet end; separating the tissue sample via the microfluidic separating system into a debris fluid volume and a sperm fluid volume; and enriching the sperm fluid volume by removing excess media via the membrane filter. A two-stage tissue sample separation system comprising: a microchannel structure defining a separation fluid channel to form a separation stage; an inlet end of the microchannel structure; an outlet end of the microchannel structure; and a membrane filter fluidly connected to the outlet end for removal of at least a portion of excess media in the tissue sample.
    Type: Grant
    Filed: October 29, 2018
    Date of Patent: December 6, 2022
    Assignee: University of Utah Research Foundation
    Inventors: Raheel Samuel, Bruce Gale, Alex Jafek, Timothy Jenkins, Jim Hotaling, Douglas Carrell, Jiyoung Son
  • Patent number: 11498256
    Abstract: A portable module for a container forming and filling system. The portable module includes a control module, an operator interface, a pump, connectors, and a transport member. The pump is configured to pump liquid for forming the container and filling the container into a container forming and filling machine. The connectors are for connecting the portable module to the container forming and filling machine. The transport member is for moving the portable module.
    Type: Grant
    Filed: April 26, 2017
    Date of Patent: November 15, 2022
    Assignee: LiquiForm Group LLC
    Inventor: G. David Lisch
  • Patent number: 11491488
    Abstract: The present invention provides a sperm sorting chip and a method for sorting sperm using the same. Said sperm sorting chip includes: a flow channel structure sequentially configured with a gradually diverging flow field region, a main flow channel, and a gradually converging main flow channel intercommunicated with each other from a first side end to a second side end; a fluid injection port, a semen injection port, and a semen extraction port separately located at the first side end and communicated with a main input channel of the gradual diverging flow field region; and a waste fluid outlet located at the second side end and communicated with the gradually converging main flow channel. The gradually diverging flow field region further includes a plurality of sub-input channels derived from the main input channel and converged into the main flow channel, and the plurality of sub-input channels have a gradually widening channel width at the junction with the main flow channel.
    Type: Grant
    Filed: May 22, 2020
    Date of Patent: November 8, 2022
    Assignee: iPreg Inc.
    Inventors: Bor-Ran Li, Chung-Hsien Huang
  • Patent number: 11474065
    Abstract: An object of the present invention is to provide a biomolecule measuring device that can decrease the influence of crosstalk between chambers. A biomolecule measuring device according to the present invention supplies, to electrodes equipped on chambers, voltages modulated differently to each other.
    Type: Grant
    Filed: April 11, 2017
    Date of Patent: October 18, 2022
    Assignee: Hitachi High-Tech Corporation
    Inventors: Yoshimitsu Yanagawa, Yusuke Goto, Michiru Fujioka
  • Patent number: 11454635
    Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of Coagulation factor VII, CA19-9, Insulin-like growth factor-binding protein 7, C—X—C motif chemokine 6, and C—C motif chemokine 13 as diagnostic and prognostic biomarkers in renal injuries.
    Type: Grant
    Filed: February 4, 2011
    Date of Patent: September 27, 2022
    Assignee: Astute Medical, Inc.
    Inventors: Joseph Anderberg, Jeff Gray, Paul McPherson, Kevin Nakamura, James Patrick Kampf
  • Patent number: 11447751
    Abstract: A method of circulating tumor cells isolation, using an isolating cultural system of circulating tumor cells, comprises the following steps: (1) providing a sample; (2) adding a cell culture medium to the isolating cultural system of circulating tumor cells; (3) adding the sample to the isolating cultural system of circulating tumor cells to cultivate; and (4) collecting the suspended circulating tumor cells in the cell culture medium; wherein the isolating cultural system of circulating tumor cells comprises a container including a cell adhesion portion, and cellulose coated on the cell adhesion portion.
    Type: Grant
    Filed: September 4, 2019
    Date of Patent: September 20, 2022
    Inventors: Tai-Horng Young, Wan-Chen Huang, Hao-Ying Hsieh, Shyh-Chyi Lo, Ke-Cheng Chen, Jin-Shing Chen, Yin-Tzu Chen
  • Patent number: 11377631
    Abstract: A culture container linkage device according to the present disclosure includes a first actuator configured to advance or retract needles 32, an actuator holder rotatably provided on a frame and configured to hold the first actuator, a second actuator, and a washer configured to wash the needles. The second actuator rotates the needles via the actuator holder. The needles are configured to be positioned, by the second actuator, at a container-facing position at which the needles face a culture container and a washing-facing position at which the needles face the washer.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: July 5, 2022
    Assignee: Sinfonia Technology Co., Ltd.
    Inventors: Toshifumi Kitahara, Kunitada Hatabayashi, Kiyoshi Mori, Hirotsugu Shiraiwa
  • Patent number: 11372010
    Abstract: An analysis system 1 includes analysis apparatuses 2A to 2E which are dispersively installed in a plurality of installation places and are connected through a network 3 so as to be communicable with each other.
    Type: Grant
    Filed: July 28, 2019
    Date of Patent: June 28, 2022
    Assignee: FUJIFILM Corporation
    Inventor: Yoshinari Oda
  • Patent number: 11326199
    Abstract: A method for amplification in a microfluidic assay, includes binding an analyte to a transport element, wherein the transport element includes a particle and a first antibody element and the analyte is bound to the first antibody element; moving the transport element with the analyte towards a tracer storage site including tracer elements which includes a first binder element, a label element and a second antibody element; binding the analyte to the second antibody element; moving the transport element with the analyte and the tracer element towards a tracer transport site including second binder elements; binding the first binder element with a second binder element; moving the transport element with the analyte towards the tracer storage site so that the analyte is detached from the second antibody element of the tracer element while the first binder element remains bound to the second binder element.
    Type: Grant
    Filed: June 4, 2021
    Date of Patent: May 10, 2022
    Inventors: Jussi Hiltunen, Prateek Singh
  • Patent number: 11325321
    Abstract: Method and apparatus are disclosed for mechanically opening a heat-bonded connection site between two hollow, flexible, thermoplastic segments of a medical fluid flow path, the heat-bonded connection site having an axis. The connection site is compressed between two facing surfaces, and the facing surfaces are relatively moved to rotate the connection site about the connection site axis and to apply force to the connection site substantially perpendicular to the connection site axis.
    Type: Grant
    Filed: January 13, 2021
    Date of Patent: May 10, 2022
    Assignee: Fenwal, Inc.
    Inventors: Benjamin E. Kusters, Kyungyoon Min
  • Patent number: 11319575
    Abstract: An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: May 3, 2022
    Assignee: BD KIESTRA B.V.
    Inventor: Raphael Rodolphe Marcelpoil
  • Patent number: 11319339
    Abstract: Disclosed is a multi-channel peptide synthesizer, including a gas-bath thermotank, a plurality of reactor tubes, a motor, a rotating rack, a liquid-feeding tube, a feeding device, a vacuum tube and a nitrogen tube. The gas-bath thermotank body provides a desired constant temperature for reaction. The reactor tube provides a place for peptide synthesis and resin washing. The motor and the rotating rack are used to fully mix the reaction and cleaning solutions. Various liquid reagents required are fed to the reactor tube through the liquid-adding tube. Various materials required are prepared in advance in the feeding device and directly fed to the reactor tube. The reaction or washing solution in the reactor tube is pumped to a waste liquid tank through the vacuum tube. Nitrogen is introduced into each reactor tube through the nitrogen tube. This device can be applied in batch-wise peptide synthesis using solid-phase methods.
    Type: Grant
    Filed: June 12, 2019
    Date of Patent: May 3, 2022
    Inventors: Xiaofang Luo, Yulu Gong, Zuodong Qin, Zongcheng Wang, Pingkai Ouyang
  • Patent number: 11305283
    Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: April 19, 2022
    Assignee: Berkeley Lights, Inc.
    Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
  • Patent number: 11221475
    Abstract: A coverslip for cell culture, capable of achieving seamless adhesion between the coverslip for cell culture and a target culture device, so as to solve the technical problem that cell suspension flows into gaps between the coverslip and a culture dish. Moreover, the coverslip for cell culture has exquisite structure, and is easy to carry out batch experiment and comparison. The coverslip is flake-like with upper and lower surfaces being parallel to each other, and comprises: a coverslip substrate (1) and sub-coverslips (2); the coverslip substrate (1) is provided with at least N hollowed-out parts (11); the sub-coverslips (2) are fixedly connected in the hollowed-out parts (11) by means of M fragile connecting pieces (3), wherein both N and M are natural numbers which are not related to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: January 11, 2022
    Inventors: Xiangyuan Fang, Huilun Li, Yejames Yuan, Jianhua Yuan
  • Patent number: 11168293
    Abstract: Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first structure.
    Type: Grant
    Filed: May 17, 2018
    Date of Patent: November 9, 2021
    Assignee: Dexerials Corporation
    Inventors: Hirokazu Odagiri, Yasuyuki Kudo, Rishabh Gupta, Keiji Honjo
  • Patent number: 11118156
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Grant
    Filed: September 24, 2018
    Date of Patent: September 14, 2021
    Inventors: Phillip Belgrader, Benjamin Hindson
  • Patent number: 11085855
    Abstract: A method for performing a laser microdissection for cutting a dissectate from a specimen using a laser includes the step of providing the specimen in a light path of an illumination system. The specimen is illuminated by the illumination system. A detector detects light emanating from the specimen. The light detected by the detector is analyzed. It is determined, based on the analysis of the light detected by the detector, whether a receptacle for collecting the dissectate is disposed in a predetermined collection position, at which the dissectate is to be collected in the receptacle after it is cut from the specimen. Laser cutting of the dissectate from the specimen is initiated based on it having been determined that the receptacle is in the predetermined collection position.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: August 10, 2021
    Inventors: Falk Schlaudraff, Andrew K. Lee
  • Patent number: 11073519
    Abstract: In a lateral flow test (LFT) device, the liquid conduit element 20 is formed from a substrate 21, 22 or 24 having at least two layers, including a first layer 21b, 22b or 24b not exceeding 75 ?m in thickness formed from a porous material for wicking liquid, and a second layer 21a,22a or 24a of additional thickness formed from a generally non-porous polymer material acting as backing layer. The layered arrangement is incorporated into an LFT device to reduce sample volume requirements.
    Type: Grant
    Filed: March 17, 2015
    Date of Patent: July 27, 2021
    Assignee: Global Life Sciences Solutions Germany GmbH
    Inventors: Alexander Schenk, Klaus Hochleitner, Suzana Kiel, Marcel Thieme, Wei Sun
  • Patent number: 11065616
    Abstract: Microfluidics has advanced in terms of designs and structures, however, fabrication methods are either time consuming or expensive to produce, in terms of the facilities and equipment needed. A fast and economically viable method is provided to allow, for example, research groups to have access to microfluidic fabrication. Unlike most fabrication methods, a method is provided to fabricate a microfluidic device in one step. In an embodiment, a resolution of 50 micrometers was achieved by using maskless high-resolution digital light projection (MDLP). Bonding and channel fabrication of complex or simple structures can be rapidly incorporated to fabricate the microfluidic devices.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: July 20, 2021
    Inventors: Ulrich Buttner, Yousof Mashraei, Sumeyra Agambayev, Khaled N. Salama
  • Patent number: 11022888
    Abstract: The formation of microporous surfaces through polymer induced phase separation in a photopolymer solvent mixture using photopolymerization via light self-focusing and self-trapping. The self-trapping of light sets fixed regions of brightness and darkness, sustained by the polymerization of light, and then wave guiding within the substrate. Phase separation occurs with the solvent phase separating in the regions of darkness and crosslinking in the regions of brightness. Upon removal of the solvent, precise and uniformly dispersed pores are created in the surface. The pore size and spacing may be tuned by adjusting the weight fraction of the photopolymer solvent mixture as well as through changes in the mask pattern.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: June 1, 2021
    Assignee: Syracuse University
    Inventor: Ian D. Hosein
  • Patent number: 10988794
    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.
    Type: Grant
    Filed: February 20, 2019
    Date of Patent: April 27, 2021
    Inventors: Samad Talebpour, Aye Aye Khine, Tino Alavie, Stephen Wesley Leonard
  • Patent number: 10973247
    Abstract: In a food taste reproduction system, a taste recognition device obtains the taste data obtained by quantifying the food taste. A first terminal converts the taste data into taste reproduction data on a component ratio of taste components for reproducing the food taste by combining a plurality of the taste components, the taste component containing a material providing at least one basic taste. The first terminal transmits the taste reproduction data to the server for storing. A second terminal receives the taste reproduction data via an Internet and outputs the taste reproduction data to a taste reproduction device. The taste reproduction device selects at least two taste components among a plurality of the taste components based on the taste reproduction data, and combines the selected taste components in accordance with the component ratio so as to obtain the taste reproduced material.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: April 13, 2021
    Assignee: DENTSU INC.
    Inventor: Ryosuke Sakaki
  • Patent number: 10947575
    Abstract: Provided are a rapid antimicrobial susceptibility test, based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and an automated cell image analysis system therefor. The antimicrobial susceptibility test is rapidly performed based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and this makes it possible to obtain highly reliable test results faster by six to seven times than the standard method recommended by Clinical and Laboratory Standards Institute (CLSI).
    Type: Grant
    Filed: November 27, 2018
    Date of Patent: March 16, 2021
    Inventors: Yong-Gyun Jung, Eun-Geun Kim, Jung Heon Yoo, Sunghoon Kwon, Jungil Choi, Hee Chan Kim, Jung Chan Lee, Eui Jong Kim, Sang Hoon Song, Sei Ick Joo, Ji Soo Lee
  • Patent number: 10921218
    Abstract: A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: February 16, 2021
    Assignee: Life Technologies Corporation
    Inventors: Joel Colburn, Mousumi Rath, Sarah Ghanbari
  • Patent number: 10894806
    Abstract: The invention relates to a process for the selective concentration of immunoglobulins or other proteins that contain an Fc domain (target protein), comprising the following steps: a. preparing a solution that contains the target protein; b. incorporating an Fc-binding protein with precisely two binding sites under conditions that allow binding to occur; c. separating the precipitate from the liquid phase; d. undoing the binding of the target protein from the Fc-binding protein.
    Type: Grant
    Filed: January 10, 2014
    Date of Patent: January 19, 2021
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Dorothee Ambrosius, Michael Dieterle, Philine Dobberthien, Maria-Katharina Wiegeshoff
  • Patent number: 10808009
    Abstract: Methods and compositions related to the selective, specific disruption of multiple ligand-receptor signaling interactions, such as ligand-receptor interactions implicated in disease, are disclosed. These interactions may involve multiple cytokines in a single receptor family or multiple ligand receptor interactions from at least two distinct ligand-receptor families. The compositions may comprise polypeptides having composite sequences that comprise sequence fragments of two or more ligand binding sites. The methods and compositions may involve sequence fragments of two or more ligand binding sites that are arranged to conserve the secondary structure of each of the ligands from which the sequence fragments were taken.
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: October 20, 2020
    Assignee: BIONIZ, LLC
    Inventors: Yutaka Tagaya, Nazli Azimi
  • Patent number: 10800652
    Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: October 13, 2020
    Assignee: Berkeley Lights, Inc.
    Inventors: Keith J. Breinlinger, Daniele Malleo, Gaetan L. Mathieu, J. Tanner Nevill, Alexander H. Slocum, Mark P. White
  • Patent number: 10760041
    Abstract: The invention discloses a flexible bag for cell cultivation, comprising a cultivation compartment and at least one dialysis compartment, wherein the dialysis compartment(s) is/are delimited from the cultivation compartment by one or more dialysis membranes and is/are fluidically connected to a first and a second port in the bag.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: September 1, 2020
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Karl Liderfelt, Eric Faldt, Andreas Castan
  • Patent number: 10723999
    Abstract: The present invention relates to a process for in vitro spermatogenesis from male germinal tissue comprising conducting maturation of testicular tissue comprising germ cells in a bioreactor which is made of a biomaterial and comprises at least one cavity wherein the germinal tissue is placed, and recovering elongated spermatids and/or spermatozoa.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: July 28, 2020
    Inventors: Marie-Hélène Perrard, Philippe Durand, Laurent David
  • Patent number: 10655107
    Abstract: Provided are a method capable of evaluating adherent cells under an environment similar to an in vivo environment by a culture method similar to a two-dimensional culture, and applications thereof. An adherent cell culture method uses, as a culture chamber (10), a chamber in which two or more culture spaces each having an equivalent diameter (D) that is 1 to 5 times the diameter of a desired spheroid and each having a height (H) that is 0.3 to 5 times the equivalent diameter are arranged and a surface of each of the culture spaces has a water contact angle of 45 degrees or less. Spheroids of adherent cells are cultured in the respective culture spaces (11) arranged in the culture chamber (10).
    Type: Grant
    Filed: September 20, 2012
    Date of Patent: May 19, 2020
    Assignee: Corning Incorporated
    Inventors: Yoko Ejiri, Satoru Ayano, Masaya Hosoda, Go Tazaki
  • Patent number: 10639597
    Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: May 5, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
  • Patent number: 10633625
    Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: April 28, 2020
    Assignee: Terumo BCT, Inc.
    Inventors: Brian J. Nankervis, Mark E. Jones
  • Patent number: 10625220
    Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: April 21, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
  • Patent number: 10620198
    Abstract: The invention concerns a cartridge for performing sample analysis in a portable point of care test device (POCT); a point of care assay device, comprising said cartridge and a reader for accommodating said cartridge, for detecting and/or quantifying an analyte in a sample; and a kit of parts comprising the said components of said point of care assay device.
    Type: Grant
    Filed: August 29, 2017
    Date of Patent: April 14, 2020
    Assignee: Invitron Limited
    Inventors: Andrew James Woodhead, James Stuart Woodhead
  • Patent number: 10597713
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Grant
    Filed: April 3, 2018
    Date of Patent: March 24, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Patent number: 10585091
    Abstract: Provided herein are methods, compositions, and devices for the identification and quantification of analytes, such as nucleic acids, proteins, cells or other biological samples. The methods, compositions, and devices are suited for accurate, portable analysis of small amounts of analyte.
    Type: Grant
    Filed: January 19, 2016
    Date of Patent: March 10, 2020
    Assignees: The University of Chicago, California Institute of Technology
    Inventors: Rustem F. Ismagilov, Stephanie McCalla, Travis Schlappi, Toan Huynh, Justin Rolando, Weishan Liu, Shencheng Ge, Jason E. Kreutz
  • Patent number: 10557165
    Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: February 11, 2020
    Inventors: Koji Hashimoto, Keiko Ito, Mika Inada
  • Patent number: 10544450
    Abstract: The invention relates to a method for the duplication of nucleic acids by means of a polymerase chain reaction, in the case of which a cycle consisting of the steps of denaturing, annealing and elongation is repeatedly performed. In one embodiment, the yield (g) of specimens of a nucleic acid to be duplicated, at the end of at least one passage of the cycle, is less than 80 percent of the specimens of the nucleic acid present at the beginning of said passage and, in the case of at least one passage of the cycle, the reaction time (tA) is less than one second. In addition, in a further embodiment, the number (k) of passages of the cycle of the polymerase chain reaction is greater than 45 and/or in at least one of the passage the cycle time tc is less than 20 seconds.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: January 28, 2020
    Assignee: GNA Biosolutions GmbH
    Inventors: Federico Buersgens, Joachim Stehr, Lars Ullerich
  • Patent number: 10486156
    Abstract: Methods and systems for processing fluids utilizing a digital microfluidic device and transferring droplets from the digital microfluidic device to a downstream analyzer are described herein. Methods and systems in accordance with the present teachings can allow for the withdrawal of fluid from a digital microfluidic device, and can in some aspects enable the integration of a digital microfluidic device as a direct, in-line sample processing platform from which a droplet can be transferred to a downstream analyzer.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: November 26, 2019
    Assignees: DH Technologies Development Pte. Ltd., University of Toronto
    Inventors: John Lawrence Campbell, Kihwan Choi, Yves LeBlanc, Chang Liu, Aaron Wheeler
  • Patent number: 10464020
    Abstract: The present disclosure relates to a hydrogen peroxide vapor decomposition and detoxification system including a hydrogen peroxide vapor suction unit which sucks hydrogen peroxide vapor in a target space to be sterilized, a hydrogen peroxide vapor decomposition and detoxification unit which decomposes the sucked hydrogen peroxide vapor, a vapor discharge unit which discharges decomposition products of the hydrogen peroxide vapor, and a control unit which controls an amount of vapor suction of the vapor suction unit, decomposition and detoxification of the hydrogen peroxide vapor decomposition and detoxification unit, and the vapor discharge unit.
    Type: Grant
    Filed: January 25, 2019
    Date of Patent: November 5, 2019
    Inventors: Kang Bong Lee, Yeong Cheon Kim, Yun Sik Nam, Jin Oh Jeon, Jong Ho Park