Involving Lysis Of A Microorganism By Means Other Than Comminution Patents (Class 435/306.1)
  • Patent number: 10888260
    Abstract: A biological fluid collection device that allows a blood sample to be collected anaerobically is disclosed.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: January 12, 2021
    Assignee: Becton, Dickinson and Company
    Inventors: Adam Edelhauser, Anthony V. Torris, Robert G. Ellis, Bradley M. Wilkinson, Joseph Nathan Pratt, Bartosz Marek Korec
  • Patent number: 10784100
    Abstract: A processing chamber system includes a substrate mounting module configured to secure a substrate within a first processing chamber. The system also includes a first deposition module configured to apply a light-sensitive film to a front side surface of the substrate, and a second deposition module configured to apply a film layer to a backside surface of the substrate. The front side surface is opposite to the backside surface of the substrate. A substrate has a bare backside surface with a first coefficient of friction. A film layer is formed onto the backside surface of the substrate. The film layer formed on the backside surface of the substrate has a second coefficient of friction. The second coefficient of friction is lower than the first coefficient of friction.
    Type: Grant
    Filed: July 14, 2017
    Date of Patent: September 22, 2020
    Assignee: Tokyo Electron Limited
    Inventor: Hoyoung Kang
  • Patent number: 10241054
    Abstract: A system for use in rapid sample analysis that includes a biosensor reagent, wherein the biosensor reagent includes living biological cells; a reservoir card, wherein the reservoir card stores the biosensor reagent; and a test cartridge base, wherein the test cartridge base is configured to accept the reservoir card, and wherein the test cartridge base further includes a contoured reaction chamber; and an inlet channel connected to and entering the reaction chamber at a predetermined angle thereto.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: March 26, 2019
    Assignee: Fundamental Solutions Corporation
    Inventors: Marvin R. Williams, Charles McBrairty, Daniel W. Pfautz, Thomas J. Zupancic, Lingchun Zeng, Andrew Weiman, Richard S. Brody, Joseph D. Kittle, Anthony Truscott, Robert Baranowski
  • Patent number: 10168239
    Abstract: A gas sensing device is provided including an enclosure having an internal chamber operable to receive and contain at least one test gas, a photonic heat source positioned to deliver photonic energy into the internal chamber, and a pressure sensor functionally coupled to the internal chamber and operable to detect a pressure change within the internal chamber.
    Type: Grant
    Filed: December 24, 2014
    Date of Patent: January 1, 2019
    Assignee: Intel Corporation
    Inventor: Kadhair Al-Hemyari
  • Patent number: 9618139
    Abstract: An apparatus for providing thermal and magnetic energy to a receptacle containing a reaction mixture and a magnetic retention member. The apparatus can also control heating of a reaction mixture, and bring about a separation of magnetic particles from the reaction mixture. The reaction mixture typically comprises polynucleotides from a biological sample that are being brought into a PCR-ready form.
    Type: Grant
    Filed: July 23, 2008
    Date of Patent: April 11, 2017
    Assignee: Handylab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 9556485
    Abstract: The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.
    Type: Grant
    Filed: March 2, 2015
    Date of Patent: January 31, 2017
    Assignee: AVIVA BIOSCIENCES CORPORATION
    Inventors: Ping Lin, Douglas T. Yamanishi, George Walker, Junquan Xu, Mingxian Huang, Guoliang Tao, Lei Wu, Xiaobo Wang, Joe Ouyang, Jing Cheng, Jia Xu
  • Patent number: 9260475
    Abstract: Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Heating may be used. Lysing may be performed in a flow through apparatus.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: February 16, 2016
    Assignee: Claremont BioSolutions LLC
    Inventors: Bruce Irvine, Robert W. Doebler, Barbara Erwin, Ryan P. Talbot, Angelika Brigitte Niemz
  • Patent number: 9089640
    Abstract: A portable and highly automated apparatus and method for introducing at least one compound within erythrocytes; the apparatus comprises a reusable part provided with mechanical elements such as pumps and valves and electronic units such as a control unit; the apparatus also comprises a disposable part, which is adapted to come into contact with the sample containing the erythrocytes and is provided with a system of tubes made of deformable material, a plurality of reservoirs and one or more filters; the apparatus allows a further concentration of the erythrocytes after they have been treated; the apparatus allows to introduce the compound in the erythrocytes in a virtually totally automated manner.
    Type: Grant
    Filed: April 26, 2011
    Date of Patent: July 28, 2015
    Assignee: ERYDEL S.p.A.
    Inventors: Giovanni Mambrini, Sonja Serafini
  • Publication number: 20150140565
    Abstract: A method for digesting biological cells in a reaction container includes treating the cells with pressure pulses. The pressure pulses are generated by at least one eddy current actuator in conjunction with an electric conductor arranged on or in the reaction container.
    Type: Application
    Filed: March 7, 2013
    Publication date: May 21, 2015
    Inventors: Uwe Iben, Peter Rothacher, Robert Giezendanner-Thoben
  • Publication number: 20150132841
    Abstract: A vessel holder assembly is provided. A vessel holder, for receiving one or more vessels, includes a first set of attachment features. A cap assembly, for covering the vessel(s) includes a second set of attachment features. Respective attachment features of the vessel holder and the cap assembly may be complementary, such that engagement between complementary attachment members results in an attachment between the cap assembly and the vessel holder. Such an attachment may be manually removable, or may be locked so as to require a tool, or other suitable method, for detaching the cap assembly from the vessel holder.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 14, 2015
    Applicant: Covaris, Inc.
    Inventors: Jonathan Sampson, James A. Laugharn, JR., Ion A. Tsinteris
  • Publication number: 20150132793
    Abstract: A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample.
    Type: Application
    Filed: June 26, 2013
    Publication date: May 14, 2015
    Applicant: Biocartis NV
    Inventors: Roel Penterman, Bart Van Meerbergen
  • Publication number: 20150111215
    Abstract: The invention provides containers and methods of use for the storage, transportation and preparation of samples, such as DNA samples for analysis. The container is pre-provided with the reagents in sealed chambers. The sample can be introduced and the container manipulated to release the reagents, provide the necessary conditions and give a fully prepared sample. The container can then be engaged with an analysis device to identify characteristics of the sample or perform other operations thereon.
    Type: Application
    Filed: May 21, 2014
    Publication date: April 23, 2015
    Applicant: FORENSIC SCIENCE SERVICE LIMITED
    Inventors: Gary ROSS, Sebastian WETHERALL, Owen EVANS, Andrew HOPWOOD, Nina MORAN
  • Publication number: 20150104825
    Abstract: A cell capture, disruption, and extraction apparatus includes a disruption chamber configured to receive cell solution and having therein a plurality of abrasives, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The apparatus includes an actuation device configured to agitate the disruption chamber and/or pestle, movement of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. The pestle can rotate with respect to the disruption chamber. The pestle can include a nut-shaped core or axle, and/or include a plurality of extrusions. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content through the foregoing agitation process.
    Type: Application
    Filed: October 11, 2014
    Publication date: April 16, 2015
    Inventor: Kianoosh Peyvan
  • Patent number: 8999704
    Abstract: Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by positioning a suitable element so as to reduce and/or eliminate a headspace size at a sample/gas interface. An interaction between the acoustic energy and the sample may be controlled, e.g., by using a headspace control element positioned at least partially in the sample that helps to reduce splashing or other sample ejection that would otherwise occur.
    Type: Grant
    Filed: July 20, 2011
    Date of Patent: April 7, 2015
    Assignee: Covaris, Inc.
    Inventor: James A. Laugharn, Jr.
  • Patent number: 8993292
    Abstract: Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container.
    Type: Grant
    Filed: May 8, 2008
    Date of Patent: March 31, 2015
    Assignee: Applied Biosystems LLC
    Inventor: Yingjie Liu
  • Publication number: 20150087048
    Abstract: A method and apparatus for processing microorganisms is provided. The method comprises mixing microorganisms with a working fluid to form an working fluid slurry, and injecting a transport fluid through a transport fluid nozzle into the working fluid slurry in order to disrupt the cellular structure of the microorganisms.
    Type: Application
    Filed: June 22, 2012
    Publication date: March 26, 2015
    Inventors: Marcus Brian Mayhall Fenton, Olga Koroleva, Michelle Gina Elizabeth Gothard, Christopher Drake
  • Patent number: 8986986
    Abstract: A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: March 24, 2015
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Kyu-youn Hwang, Joon-ho Kim, Sung-hong Kwon, Chin-sung Park, Hee-kyun Lim, Sun-ok Jung, Won-jong Jung
  • Publication number: 20150079655
    Abstract: Methods and systems for treating material with focused acoustic energy and/or ultraviolet radiation are described. Sample material may be provided to a single batch vessel or may flow in a continuous or intermittent fashion into/out of a processing chamber within which the material is exposed to focused acoustic energy and/or ultraviolet radiation. Exposure to focused acoustics in a flow through environment may enhance the effects of the radiation on the sample material. Further, in flow-through or single batch arrangements, sample material may be retained exterior to or within a processing chamber by one or more retaining members (e.g., filter, valve, etc.) until a characteristic of the sample material reaches one or more particular criteria (e.g., physical, chemical, biological). Once the criteria of the sample material is attained (e.g., via further treatment), the material is then permitted to flow past the retaining member(s) and through the inlet or outlet of the chamber.
    Type: Application
    Filed: September 17, 2013
    Publication date: March 19, 2015
    Applicant: Covaris, Inc.
    Inventors: James A. Laugharn, JR., Xiaoyin He, Srikanth Kakumanu
  • Patent number: 8969021
    Abstract: The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: March 3, 2015
    Assignee: AVIVA Biosciences Corporation
    Inventors: Ping Lin, Douglas T. Yamanishi, George Walker, Junquan Xu, Mingxian Huang, Guoliang Tao, Lei Wu, Xiaobo Wang, Joe Ouyang, Jing Cheng, Jia Xu
  • Publication number: 20150044679
    Abstract: Disclosed herein are systems, devices, and methods for detecting the presence of a pathogen in a biological host, such as in a point of care setting. In certain aspects, materials and methods improve point of care devices by providing pre-loaded, preferably dried, agents for performing one or more of sample lysis and signal enhancement inside the device.
    Type: Application
    Filed: August 7, 2014
    Publication date: February 12, 2015
    Inventors: Graham D. Jack, Susan Bortolin
  • Publication number: 20150044696
    Abstract: An integrated microfluidic device for carrying out a series of fluidic operations includes a housing including a plurality of n microfluidic conduits, wherein n is at least three, and a rotating valve having an internal channel with an entrance port and an exit port that are angularly separated. The rotating valve is positionable in a first position to connect two of the n fluidic conduits via the internal channel, and upon rotating the valve to a second position, two other of the n fluidic conduits are connected by the internal channel. The device further may include one or more fluidic chambers in fluid communication with respective fluidic conduits. Fluid contained in one fluidic chamber is transferrable by application of positive or negative gas pressure through associated fluidic conduits into another fluidic chamber via the internal channel. The device may be utilized to perform a variety of fluidic operations.
    Type: Application
    Filed: August 9, 2013
    Publication date: February 12, 2015
    Applicant: SHARP KABUSHIKI KAISHA
    Inventors: Pamela Ann DOTHIE, Daniel Christopher SPENCER
  • Publication number: 20150045232
    Abstract: The disclosure relates to a system diagnosis of a disease or a disease condition whereby a single sample is prepared from a biological specimen which integrates synchronously the methods of protection, isolation and alteration of a biological specimen or a bio-molecule to isolate and study tissues and bio-molecules including DNA, large RNA, small RNA, protein, lipid, carbohydrates, and other metabolite simultaneously or individually resulting in a comprehensive understanding of the cause, prevention, risk, seriousness, confirmation, treatment, triage, and prognosis of a disease or a disease condition.
    Type: Application
    Filed: March 9, 2014
    Publication date: February 12, 2015
    Applicant: BexMart
    Inventors: Bob Han, Eric Han, Xiaohui Xiong, Xiaoliang Han
  • Publication number: 20150031125
    Abstract: A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber
    Type: Application
    Filed: October 2, 2014
    Publication date: January 29, 2015
    Inventors: Kyu-youn HWANG, Joon-ho KIM, Sung-hong KWON, Chin-sung PARK, Hee-kyun LIM, Sun-ok JUNG, Won-jong JUNG
  • Publication number: 20150024480
    Abstract: Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.
    Type: Application
    Filed: August 4, 2014
    Publication date: January 22, 2015
    Inventors: Robert Doebler, Ali Nadim, James D. Sterling, Anna Hickerson, Barbara Erwin, Denice Woyski, Ryan P. Talbot, Bruce Irvine
  • Publication number: 20150024481
    Abstract: Devices and system for preparing samples are described. Such devices can comprise fluidic chambers, reservoirs, and movable structures for controlling the movement of samples. The device can also comprise functional elements for performing specific operations.
    Type: Application
    Filed: September 11, 2014
    Publication date: January 22, 2015
    Inventors: Imran R. MALIK, Axel SCHERER
  • Patent number: 8927268
    Abstract: Disclosed herein are methods for quickly obtaining crude membranes from cells, including animal and plant cells. The methods include incubating cells in a buffer and forcing the cells through a filter that causes rupture of the cells, and then separating the resulting crude membranes from most cytosolic proteins.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: January 6, 2015
    Assignee: Invent Biotechnologies, Inc.
    Inventors: Lin Yan, Quanzhi Li
  • Publication number: 20140356941
    Abstract: A cartridge configured for use in a blood analyzer is provided. The cartridge may include a substantially rigid frame, a flow path within the rigid frame, at least one opening in the substantially rigid frame configured to align and stabilize a capillary tube, and a seal within the flow path. The seal may be configured to temporarily obstruct flow through at least a portion of the flow path. The seal may also be configured to open in response to a force exerted via a capillary tube inserted into the at least one opening.
    Type: Application
    Filed: May 30, 2014
    Publication date: December 4, 2014
    Inventors: Avishay Bransky, Liron Shiomo
  • Publication number: 20140349386
    Abstract: A nucleic acid extraction device, which can realize automation, ultra-miniaturization and super-high speed in the nucleic acid extraction reaction, has no limitation to the type of biological specimens that can be used, such as sputum, blood, cells, urine, saliva, tissues, etc., minimize the used amount of the sample solution, and also maintain and/or improve the nucleic acid extraction efficiency with reliability.
    Type: Application
    Filed: August 7, 2014
    Publication date: November 27, 2014
    Inventors: Sung Woo KIM, Duck Joong KIM, Dong Hoon LEE, Sun Jin KIM, Yong Hea CHOI, Ho Sun RYU
  • Patent number: 8895297
    Abstract: A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: November 25, 2014
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Kyu-youn Hwang, Joon-ho Kim, Sung-hong Kwon, Chin-sung Park, Hee-kyun Lim, Sun-ok Jung, Won-jong Jung
  • Patent number: 8893995
    Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: November 25, 2014
    Assignee: Auxocell Laboratories, Inc.
    Inventors: Rouzbeh R. Taghizadeh, John Meade
  • Publication number: 20140342439
    Abstract: Apparatuses, kits, and methods for portable sample disruption (e.g., for encouraging cell lysis).
    Type: Application
    Filed: May 28, 2014
    Publication date: November 20, 2014
    Applicant: ZYMO RESEARCH CORPORATION
    Inventors: Nikolas M. ISELY, Marc E. VAN EDEN, Jonathan K. BROWN
  • Publication number: 20140322697
    Abstract: A multiple polymer layer test cartridge includes an input to receive a sample containing cells, multiple lysing channel structures on alternate layers of the multiple layer test cartridge coupled to each other to pass the sample in sequence between the lysing channel structures, and a test chamber to receive the sample from the multiple lysing channel structures.
    Type: Application
    Filed: April 29, 2013
    Publication date: October 30, 2014
    Applicant: HONEYWELL INTERNATIONAL INC.
    Inventors: Pamela Wong, Phillip Henning, Elizabeth Palaima
  • Patent number: 8859272
    Abstract: A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: October 14, 2014
    Assignee: Samsung Electronics, Ltd.
    Inventors: Kyu-youn Hwang, Joon-ho Kim, Sung-hong Kwon, Chin-sung Park, Hee-kyun Lim, Sun-ok Jung, Won-jong Jung
  • Publication number: 20140272983
    Abstract: The present invention relates to a method for storage and subsequent lysis of a sample in which the sample is immobilized on a solid support. The solid matrix is embedded with a low concentration of both a chaotropic salt and a surfactant which act synergistically to efficiently store and lyse a biological sample.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: GE HEALTHCARE UK LIMITED
    Inventors: HUGH O'MALLEY, BARRY JOHNSON
  • Publication number: 20140272997
    Abstract: A device for isolating DNA from a sample containing cells, including a cartridge having an entrance port and an exit port, a membrane disposed between the entrance port and the exit port, and a plurality of channels between the membrane and the exit port. Additionally, systems and methods for isolating DNA from a sample containing cells and also systems and methods for amplifying and isolating single-stranded DNA from a sample containing DNA.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Applicant: F Cubed, LLC
    Inventors: Leslie T. Ivie, Christopher Chanelli, Shaunasee Kocen
  • Publication number: 20140256929
    Abstract: An RNA extraction buffer, an RNA extraction method, and an RNA extraction kit are described which enable functional, rapid, efficient, and high-quality RNA isolation from samples containing high concentrations of aqueous metal cations, clays, silica, or silicate minerals
    Type: Application
    Filed: March 5, 2014
    Publication date: September 11, 2014
    Inventors: Andrea Loes, Shelley Haydel
  • Patent number: 8822211
    Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentially present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.
    Type: Grant
    Filed: June 11, 2013
    Date of Patent: September 2, 2014
    Assignee: Becton Dickinson Infusion Therapy Systems Inc.
    Inventors: Jean-Pierre Hermet, Isabelle Besson-Faure, Sebastien Ribault, Yann Godfrin, Anne Monnot des Angles
  • Publication number: 20140242678
    Abstract: The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample.
    Type: Application
    Filed: July 27, 2011
    Publication date: August 28, 2014
    Inventors: Andreas Boos, Gerd Lüdke, Johannes Bacher
  • Patent number: 8815521
    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: August 26, 2014
    Assignee: Cepheid
    Inventors: Michael T. Taylor, Farzad Pourahmadi, William A. McMillan, Ronald Chang
  • Publication number: 20140206060
    Abstract: A system and method for sonication of multiple samples and continuous sonication of an input fluid stream in flow-through arrangements useful for economical breakdown of particulates and organisms present in large volumes with relatively low-power sonication devices such as production of oil from algae. The system includes an electrical wave generator oscillating in the ultrasound range, a vibrating element electrically connected to the electrical wave generator, and a sonication plate that vibrates in certain modes. The sonication plate contains features for mating with sample tubes, and the sample tubes also possess complimentary mating features to those on the sonication plate. A method for sonication of multiple samples includes utilizing mating features to attach tubes to the sonication plate and energizing the sonicator to vibrate the sonication plate. The invention also relates to arrangements for continuous flow-through useful for sonicating large sample volumes.
    Type: Application
    Filed: January 18, 2013
    Publication date: July 24, 2014
    Inventor: Folim G. Halaka
  • Publication number: 20140206074
    Abstract: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
    Type: Application
    Filed: March 18, 2014
    Publication date: July 24, 2014
    Inventors: Kurt E. Peterson, William McMillan, Farzad Pourahmadi, Ronald Chang, Douglas B. Dority
  • Publication number: 20140206562
    Abstract: Fabrication of a microfluidic multi-temperature reaction device (MMR) and the design and fabrication of the equipment to drive various molecular biological methods on the device are provided. The device can be applicable, for example, to nucleic acid (DNA, RNA, cDNA, etc) amplification, cell lysis, reverse transcription and other enzymatic temperature sensitive and also temperature cycling reactions.
    Type: Application
    Filed: January 20, 2014
    Publication date: July 24, 2014
    Applicant: QUANTUMDX GROUP LIMITED
    Inventors: JOHN EDWARD MCCORMACK, ELAINE HARRINGTON WARBURTON, JONATHAN JAMES O'HALLORAN, MATTHEW DANIEL SOLOMON, DAVID JAMES BRIGGS, MINDY LEE ANDRE, MATTHIAS SCHUENEMANN
  • Publication number: 20140199744
    Abstract: According to one embodiment, the disclosure provides a system for algal cell lysis. The system may include at least one algal cell, a plurality of metal nanoparticles, and an electromagnetic radiation generator. The generator may be able to generate radio frequency or microwave radiation that excites the plurality of metal nanoparticles, resulting in lysis of the algal cell. The disclosure also provides a system for recovery of a lipid from an algal cell similar to the above system but also including a separator. The disclosure further provides a method of recovering a lipid from an algal cell by supplying a plurality of metal nanoparticles to at least one algal cell, exciting the plurality of metal nanoparticles with radio frequency or microwave electromagnetic radiation, lysing the algal cell to release the lipid, and separating the lipid from the lysed algal cell.
    Type: Application
    Filed: March 18, 2014
    Publication date: July 17, 2014
    Applicant: RAYTHEON COMPANY
    Inventors: Carlos R. Costas, Christopher R. Eck
  • Patent number: 8772017
    Abstract: Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: July 8, 2014
    Assignee: Micronics, Inc.
    Inventors: C. Frederick Battrell, John Gerdes, John R. Williford, Denise Maxine Hoekstra, Wayne L. Breidford, Stephen Mordue, John Clemmens, Melud Nabavi, Mark Kokoris
  • Publication number: 20140178982
    Abstract: A reagent supply device includes a plurality of chambers which are separated from each other such that different reagents may be injected, the plurality of chambers having bottom surfaces made of a membrane, the membrane comprising a destruction pattern which is formed at a position corresponding to each of the plurality of chambers and breachable by an external impact to discharge the injected reagent from the chamber.
    Type: Application
    Filed: June 13, 2013
    Publication date: June 26, 2014
    Inventors: Chin-sung PARK, Kak NAMKOONG, Joon-sub SHIM, Won-seok CHUNG, Kyung-ho KIM, Joon-ho KIM, Won-jong JUNG
  • Patent number: 8758990
    Abstract: The present invention relates to compositions and methods for detecting Mycobacterium genus (Mycobacterium sp.) bacteria (e.g., M. tuberculosis) and M. avium complex (MAC; M. avium, M. scrofulaceum and M. intracellulaire) in a clinical sample or culture. The present invention also relates to differentiating cultures or samples comprising (Mycobacterium sp.) bacteria (e.g., M. tuberculosis) from cultures or samples comprising M. avium complex (MAC; M. avium, M. scrofulaceum and M. intracellulaire).
    Type: Grant
    Filed: October 18, 2007
    Date of Patent: June 24, 2014
    Assignee: ID-Fish Technology, Inc.
    Inventors: Jyotsna S. Shah, Helena Weltman
  • Publication number: 20140154797
    Abstract: A lysis/resealing process for preparing erythrocytes containing active ingredient is provided comprising placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65%, with refrigeration at 1 to 8° C.; measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension; lysis and internalisation procedure of the active ingredient, inside the same chamber, at a temperature maintained at 1 to 8° C., comprising allowing the erythrocyte suspension having a haematocrit level equal to or greater than 65% and a hypotonic lysis solution which is refrigerated at 1 to 8° C., to circulate in a dialysis cartridge; the lysis parameters being adjusted in accordance with the osmotic fragility previously measured; and resealing in a second chamber at a temperature of from 30 to 40° C. by means of a hypertonic solution.
    Type: Application
    Filed: November 26, 2013
    Publication date: June 5, 2014
    Applicant: ERYTECH PHARMA
    Inventor: Yann GODFRIN
  • Publication number: 20140147862
    Abstract: A microfluidic apparatus capable of being mounted on a rotation driving unit. When mounted on the rotation driving unit and rotated, a fluid flow is induced in the apparatus due to a centrifugal force. the microfluidic apparatus includes a sample supplying unit that comprises a sample chamber to house a sample and provides a fluid including a target material in which fine beads are bound to a target cell in the sample; a density-gradient separation unit that houses a density gradient material with a lower density than the target material, and receives the fluid from the sample supplying unit to separate the target material from the fluid based on a density difference; a fluid supplying channel connecting the sample supplying unit to the density-gradient separation unit to form a passage of the fluid; and a fluid supplying valve provided to the fluid supplying channel to selectively allow a flow of the fluid.
    Type: Application
    Filed: September 13, 2013
    Publication date: May 29, 2014
    Inventors: Min Seok S. KIM, Jong-myeon PARK
  • Publication number: 20140127773
    Abstract: Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. Binding of biological materials to solid surfaces may be induced by particular media. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Lysing materials may include particles of different sizes. Heating may be used. Lysing may be performed in a flow through apparatus. Surfaces of solid materials may be modified to capture bacteria with high cell wall lipid content.
    Type: Application
    Filed: January 14, 2014
    Publication date: May 8, 2014
    Inventors: Mark Brown, Robert Doebler, Bruce Irvine, Tanya Ferguson, Gary Blackburn
  • Publication number: 20140120599
    Abstract: A device configured for lysing a sample includes a holding element configured to hold an energy-transmitting element such that the holding element lies around the held part of the energy-transmitting element and envelops the held part of the energy-transmitting element and/or that the holding element lies against the held part of the energy-transmitting element. The holding element is further configured to cause lysis with the energy-transmitting element when the holding element is immersed into the sample to be subjected to lysis or comes into contact with the sample to be subjected to lysis. A method includes inserting the energy-transmitting element into the holding element. A system configured for lysing a sample by way of ultrasound includes the device and the energy transmitting element.
    Type: Application
    Filed: October 24, 2013
    Publication date: May 1, 2014
    Applicant: Robert Bosch GmbH
    Inventors: Martina Daub, Jochen Rupp, Peter Rothacher