Microorganism Preservation, Storage, Or Transport Apparatus Patents (Class 435/307.1)
  • Patent number: 9423325
    Abstract: A block storage device includes: a magazine which accommodates embedding blocks to be inserted into or removed from the magazine in a state where the embedding blocks held in a cassette to which ID data is attached are arranged vertically in one row; a magazine holding portion which detachably holds each of the magazines; and a reading portion which reads the ID data. The magazine holding portion includes a rotary body which is rotatable around a rotation axis O, a holding mechanism which is multiply provided on a circumferential wall portion in the rotary body and detachably holds the magazines individually, and a rotation drive portion which positions one of the magazines at a block extraction position facing the reading portion by rotating the rotary body, and the magazine positioned at the block extraction position and the reading portion are movable relative to each other along the vertical direction.
    Type: Grant
    Filed: November 5, 2013
    Date of Patent: August 23, 2016
    Assignee: SAKURA FINETEK JAPAN CO., LTD.
    Inventors: Jyunya Enomoto, Seigo Murakami, Nobuyuki Nakagawa
  • Patent number: 9291533
    Abstract: The present invention relates to a method for processing isolated cells, in particular rare cells such as circulating tumor cells, to render them suitable for optical imaging, comprising the steps of (a) depositing filter material comprising captured cells on a phase-change medium; (b) melting said phase-change medium until the medium is spread out below said filter material; and (c) lowering the temperature of said phase-change medium until the medium solidifies, resulting in an optically flat filter comprising said cells at a fixed position. The method may additionally comprise an initial step of capturing a cell on a filter material. The phase-change medium, preferably paraffin wax, may comprise a porous, or mesh-like structure allowing the passage of a fluid through the medium. The phase-change medium may further be mounted on a carrier such as a glass carrier or a polymer material carrier.
    Type: Grant
    Filed: July 4, 2012
    Date of Patent: March 22, 2016
    Assignee: Koninklijke Philips N.V.
    Inventors: Menno Willem Jose Prins, Mikhail Mikhaylovich Ovsyanko, Freek Van Hemert, Arie Rombertus Van Doorn
  • Patent number: 9125397
    Abstract: Provided are apparatuses for cryopreserving cells which include a vessel comprising a biocompatible substrate, wherein the vessel further comprises an interior and an exterior, and a mechanical ice nucleating device disposed in or on the vessel interior for initiating ice crystal formation. Also provided are kits comprising one or more apparatuses for cryopreserving cells and a biopreservation medium. Further provided are compositions comprising a vessel for holding cells, a mechanical ice nucleating device, a biopreservation medium, and cells disposed in the vessel. The apparatuses, kits, and compositions of the invention can optionally include an insulating material which is disposed on all or a portion of the vessel.
    Type: Grant
    Filed: April 29, 2010
    Date of Patent: September 8, 2015
    Assignee: BioLife Solutions, Inc.
    Inventors: Dominic M. Clarke, Ian B. Nicoud, Aby J. Mathew, Michael Rice
  • Patent number: 9040293
    Abstract: A cell culture dish, comprising: a dish with a bottom wall and a circumferential side wall standing upward from the same, a lid, which sits sealingly on the side wall in an aeration position, and means for holding the lid on the dish above the sealing position in an aeration position in which there is an aeration gap between side wall and lid, wherein these means are adapted to be overcome by pressing the lid and the dish together in order to bring the lid from the aeration position into the sealing position.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: May 26, 2015
    Assignee: Eppendorf AG
    Inventors: Nico Gülzow, Thomas Reimann, Jochen Beese, Ute Kowanz, Yvonne Radacz
  • Publication number: 20150140656
    Abstract: The present invention is a method of freezing cells comprising the steps of incubating said cells in a solution comprising a cryoprotective agent, concentrating the cells resulting from the previous step withdrawing the eluent essentially free of cells, and freezing the resulting concentrated cells. The cells frozen by the invention's method render a high post-thawing viability, reduce cryoprotectant related toxic events and promote cells life in a suspension state after thawing. The invention also comprises the container comprising the frozen cells.
    Type: Application
    Filed: September 16, 2014
    Publication date: May 21, 2015
    Inventors: Roberto HERNAN IZQUIERDO, Natalia Gallot Escobal, Antonio Cruz Pacheco
  • Publication number: 20150140650
    Abstract: An auto-nucleating device includes a tube containing a crystalline cholesterol matrix. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within, and an opposite end that is initially open to receive the liquid. Another vessel includes an adaptor mounted to liquid container with a tubular branch closed by a needle septum and another tubular branch provided with a barbed fitting for engaging a flexible tube that terminates in a needle septum. In another embodiment, the vessel includes an inlet and vent branch at the top of the container and an outlet septum at a bottom opening.
    Type: Application
    Filed: January 8, 2015
    Publication date: May 21, 2015
    Inventors: Erik John Woods, John K. Critser
  • Publication number: 20150132842
    Abstract: A package for cell-containing material includes an inner and outer bag. The periphery of the inner bag except an opening is sealed. The periphery of the outer bag except an opening is sealed. The outer bag contains the inner bag so the opening faces outward. A portion of the opening outside the inner bag is sealed and forms an outer bag sealed portion. A portion of the opening that overlaps the inner bag is sealed and forms an inter-bag sealed portion. A sterilized closed space may be surrounded by the outer surface of the inner bag and the inner surface of the outer bag. An adhesive strength of heat sealing of the inner surfaces of the opening of the inner bag may be greater than an adhesive strength of the inter-bag sealed portion and an adhesive strength of the outer bag sealed portion.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 14, 2015
    Inventor: Makoto Sugiura
  • Publication number: 20150125954
    Abstract: The invention relates to a substrate unit (100), in particular for the cryopreservation of a biological sample (1) containing biological cells (2, 2.1, 2.
    Type: Application
    Filed: July 1, 2013
    Publication date: May 7, 2015
    Inventors: Heiko Zimmermann, Axel Beier, Julia Neubauer, Guenter R. Fuhr
  • Publication number: 20150087052
    Abstract: A container includes an inner bag, where the interior of the inner bag includes a sterile environment for storing a material, at least one access port configured to provide fluid access to the interior of the inner bag, an overwrap bag, where the interior of the overwrap bag includes a sterile environment for storing the material and where the inner bag is enclosed within the interior of the overwrap bag, and an overwrap access port configured to provide fluid access to the interior of the overwrap bag. The material can include a biomaterial. Each of the inner bag, the overwrap bag, the at least one access port, and the overwrap access port can include a fluoropolymer such as fluoroethylenepropylene (FEP).
    Type: Application
    Filed: September 23, 2014
    Publication date: March 26, 2015
    Inventors: Herbert CULLIS, Phillippe J. BROUSSARD
  • Patent number: 8969075
    Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway.
    Type: Grant
    Filed: December 18, 2008
    Date of Patent: March 3, 2015
    Assignee: University of Massachusetts
    Inventor: Andrew H. Fischer
  • Patent number: 8961429
    Abstract: An arrangement for collecting a tissue or stool sample has a sample vial and a source of preservative fluid. A fluid valve controls a flow of the preservative fluid from the source of preservative fluid to the sample vial. An air equalization vent tube is coupled to the source of preservative fluid and an air valve controls a flow of air through the air equalization vent tube, the air valve and the fluid valve being actuated simultaneously. A scoop for collecting the sample is coupled to the sample vial coupler and extends into the sample vial. The preservative fluid flows from the source of preservative fluid to the sample vial by force of gravity upon actuation of the fluid valve. The air equalization vent tube transfers air from the sample vial to the source of preservative fluid upon actuation of the air valve.
    Type: Grant
    Filed: September 19, 2008
    Date of Patent: February 24, 2015
    Assignee: Wayne State University
    Inventors: Jeffrey L. Ram, Jordan Nechvatal, Ikuko Kato
  • Patent number: 8961896
    Abstract: A cytological cell sample collection, storage, and transport device is disclosed. The device comprises a sheath, a collection assembly, a base, and a containment vial. The collection assembly is slidably coupled to the base to expose a swab head comprising the collection assembly. The containment vial is configured to enclose the sheath and collection assembly within the internal volume defined by the containment vial and the base.
    Type: Grant
    Filed: November 21, 2011
    Date of Patent: February 24, 2015
    Assignee: In HindSight LLC
    Inventor: Kathy Mary McSherry
  • Publication number: 20150044765
    Abstract: A cell cryopreservation tool has a cell holding member having a body part and a cell holding part and a tubular accommodation member, closed at one end thereof, which is capable of accommodating the cell holding member. The cell holding part of the cell holding member has a long and narrow cell attaching and holding portion. The cell attaching and holding portion has a heat conductor extended in a longitudinal direction thereof. When the cell holding member is accommodated in the tubular accommodation member, the heat conductor is capable of contacting an inner surface of the tubular accommodation member.
    Type: Application
    Filed: October 1, 2012
    Publication date: February 12, 2015
    Inventor: Futoshi Inoue
  • Patent number: 8951786
    Abstract: Methods for producing construction material utilizing loose pieces of aggregate (30), enzyme producing cells, an amount of urea and an amount of calcium ions. A first solution is prepared which includes urease which is formed by enzyme producing cells. A second solution is prepared which includes urea and calcium ions. The first and second solutions are added to the loose aggregate (30). The calcium ions contribute to the formation of calcium carbonate wherein the calcium carbonate fills and bonds between at least some of the gaps between the loose pieces of aggregate forming a solid construction material (92).
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 10, 2015
    Assignee: BioMason, Inc.
    Inventor: Ginger K. Dosier
  • Publication number: 20150037782
    Abstract: Novel methods and systems for electromagnetic freezing are disclosed. An oscillating magnetic field can be applied in conjunction with a static magnetic field in order to align magnetic particles inside biological tissues. Such chains may be naturally present or artificially introduced. Needle-shaped electrodes may be used to produce ions and disturb the air layers insulating the tissues to be frozen.
    Type: Application
    Filed: July 10, 2014
    Publication date: February 5, 2015
    Inventors: Atsuko KOBAYASHI, Joseph L. KIRSCHVINK
  • Publication number: 20150037830
    Abstract: Container assembly including a container for storing a tissue sample and a cover adapted to engage with the container. The cover includes: a top member having a receptacle, a seal for sealing off the receptacle, a puncturing member for breaking the seal by displacement of the puncturing member. The container assembly has a first and a second position, wherein in the first position the container is isolated from the receptacle, and in the second position fluid communication is provided between the receptacle and the container. The container assembly includes a separator positioned between the seal and the bottom of the container, and the separator is provided with at least one aperture adapted to provide a fluid passage between the receptacle and the container.
    Type: Application
    Filed: November 11, 2011
    Publication date: February 5, 2015
    Applicant: AX-LAB INNOVATION APS
    Inventors: Ole Jakobsen, Christoffer Bay, Jacob Ilskov
  • Publication number: 20150031126
    Abstract: A tissue cutting device that is especially suited for neurosurgical applications is disclosed and described, as well as alternative systems for tissue preservation and transport. The cutting device includes an outer cannula in which a reciprocating inner cannula is disposed. A tissue collector is also provided and is in fluid communication with the lumen of the inner cannula. A temperature control sleeve may be disposed around the tissue collector to control the temperature of the tissue samples. A preservation system may be supplied that is configured to deliver fluids to tissue samples in the tissue collector. A fluid supply sleeve may be disposed about the outer cannula and is selectively positionable along the length of the outer cannula.
    Type: Application
    Filed: October 14, 2014
    Publication date: January 29, 2015
    Inventors: Joseph L. MARK, Brian C. DOUGHERTY, Alexandra SCHWIER
  • Publication number: 20150024487
    Abstract: The present invention relates to a cryopreservative device comprising an outer case, one or more layers of space for the cryo-bags, and two or more Teflon cryopreservative bags. The outer case has a cover lip for opening and closing. The Teflon cryopreservative bags are filled with a freezing resistant. In the present invention, the cryo-bags and the Teflon cryopreservative bags are crossly stacked in the cryopreservation device. The Teflon cryopreservative bags are designed to directly contact with the cryo-bags in order to obtain the effect of slow cell freezing.
    Type: Application
    Filed: January 8, 2014
    Publication date: January 22, 2015
    Applicant: Steminent Biotherapeutics, Inc.
    Inventors: Cheng-Yi CHENG, Yu-Shan WU, Yin LIANG, Wei-Kee ONG, Hui-Chun HO
  • Patent number: 8936905
    Abstract: An auto-nucleating device includes a tube containing a crystalline cholesterol matrix. The ends of the tube are closed by a membrane impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device provides a site for ice nucleation during freezing of the liquid within the vessel. The cryopreservation vessel can be a flexible vial having a closed port adapted to be pierced by a needle, and an opposite end that is initially open to receive the liquid. Another vessel includes an adaptor mounted to liquid container with a tubular branch closed by a needle septum and another tubular branch provided with a barbed fitting for engaging a flexible tube that terminates in a needle septum. Alternatively, the vessel includes an inlet and vent branch at the top of the container and an outlet septum at a bottom opening.
    Type: Grant
    Filed: July 16, 2012
    Date of Patent: January 20, 2015
    Assignee: Cook General Biotechnology LLC
    Inventors: Erik John Woods, John K. Critser
  • Patent number: 8932858
    Abstract: Hydrophobically modified polysaccharide compositions, articles incorporating the compositions, and methods for cell culture, automatic release of cells, and release of cell culture coating from a substrate or the cell culture articles incorporating the coating compositions, as defined herein.
    Type: Grant
    Filed: March 4, 2009
    Date of Patent: January 13, 2015
    Assignee: Corning Incorporated
    Inventors: Wendy A. Baker, Dana C. Bookbinder, Theresa Chang, Robert R. Hancock, Jr.
  • Publication number: 20150011000
    Abstract: The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.
    Type: Application
    Filed: September 22, 2014
    Publication date: January 8, 2015
    Inventor: Tomas Stojanov
  • Patent number: 8921096
    Abstract: The invention concerns a pump cart for a biological liquid treatment installation, having a first lateral face (40), and a second lateral face (41) by which it is configured to be juxtaposed against a conveying network cart (1) of the installation and a front face (42) meeting the lateral faces (40, 41), and comprising at least one pump (414), a pump support (50) on which is mounted said pump (414), and a guide member for making said pump (414) movable in translation and on which said support (50) is mounted; said support (50) being movable in translation in a direction going from the first lateral face (40) towards the second lateral face (41); whereby said pump (414) is disposed at a predetermined location on said cart (2) depending on the type of treatment carried out.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: December 30, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Jean-Louis Weissenbach, Rene Reinbigler, Virginie Buisson, Christine Abouayad El Idrissi, Sebastien Cirou
  • Publication number: 20140363843
    Abstract: Systems and methods for processing tissue samples using acoustic energy. The tissue sample may be collected and placed on a substrate (e.g., microscope slide), or other sample holder. A transfer substrate may be positioned on the other side of the sample opposite the substrate. A microscope incorporated with the acoustic treatment system may be used to view, identify and select a portion of the sample to be transferred from the initial substrate to the transfer substrate. The selected portion of the sample is exposed to focused acoustic energy while disposed between the two substrates. The focused acoustic energy has characteristics (e.g., high frequency, small focal zone) that may be effective to transfer the selected portion of the sample from the initial substrate to the transfer substrate. Such transfer may occur with little to no damage to the sample, for example, at low temperature isothermal conditions and/or little to no cavitation of or around the sample.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 11, 2014
    Inventor: James A. Laugharn, JR.
  • Patent number: 8906229
    Abstract: The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: December 9, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 8900842
    Abstract: The invention relates to a method of storing and/or transporting in vitro two-dimensional cell cultures. The inventive method comprises the following steps consisting in: a) coating a cell culture that is immobilized on an asymmetric support with a gelatine solution in culture medium at a concentration of between 1 and 5%; b) solidifying the gelatine added to the support at a temperature of between 15 and 25° C.; and c) storing and/or transporting the cell culture at a temperature of between 15 and 25° C. for a period of up to 96 hours. The invention also relates to a kit which is used to store and/or transport the in vitro two-dimensional cell cultures according to the inventive method, said kit comprising: i) an asymmetric support, and (ii) a gelatine solution in culture medium at a concentration of between 1 and 5%.
    Type: Grant
    Filed: March 29, 2004
    Date of Patent: December 2, 2014
    Assignee: Advanced In Vitro Cell Technologies, S.L.
    Inventors: Myriam Fabre, Sonia Gonzalez Menoyo, Mariana Lopez Matas, Roser Pagan I Esquius
  • Patent number: 8900857
    Abstract: A mould and tissue cassette for automated embedding of tissue, and a method of operation are disclosed. The mould attaches to the cassette prior to embedding, and fits into a rack. The rack supports the mould holding the tissue cassette when the mould and cassette are in a first position. When the mould and cassette are moved to a second position, a projection on the rack separates the mould from the cassette, to facilitate easy removal. Also disclosed is a mould for a tissue cassette having projections for holding the mould to a rack, so that the tissue cassette is not in contact with the mould.
    Type: Grant
    Filed: June 18, 2009
    Date of Patent: December 2, 2014
    Assignee: Leica Biosystems Melbourne Pty Ltd
    Inventors: Ian Emil Sohn, Chester Henderson, Timothy Brett McDonald, Richard John Louis Gardner
  • Patent number: 8900454
    Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first shell (16) disposed on a front face (5) of said base (2) and a second shell (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20).
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: December 2, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 8900856
    Abstract: Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.
    Type: Grant
    Filed: April 8, 2005
    Date of Patent: December 2, 2014
    Assignee: Biomatrica, Inc.
    Inventors: Judy Muller-Cohn, Rolf Muller, Corinna Herrnstadt
  • Publication number: 20140342454
    Abstract: Disclosed herein are devices, methods, systems and kits adapted for vitrification and/or reanimation of oocytes, embryos or blastocysts. The device includes a straw and a filter, wherein the straw comprises a lumen traversing through the straw and has a proximal section, a middle section and a distal section and wherein the filter is affixed in the straw and comprises a plurality of pores having a diameter smaller than the diameter of said oocytes, embryos or blastocysts but large enough to allow the passage of a fluid composition therethrough.
    Type: Application
    Filed: May 15, 2014
    Publication date: November 20, 2014
    Applicant: Mariposa Biotechnology, Inc.
    Inventors: Fred Burbank, Michael Jones, Carl Swindle
  • Publication number: 20140335555
    Abstract: The present application relates to devices for the vitrification of biological samples, including embryos, oocytes and biological tissues. Suitably, the devices are automated devices which require little if any operator intervention or sample handling, thereby reducing contamination, improving success rates and increasing efficiency. Also provided are methods of vitrifying a biological sample, suitably using the disclosed devices.
    Type: Application
    Filed: August 3, 2012
    Publication date: November 13, 2014
    Applicant: University of Kansas
    Inventors: S. Samuel Kim, Sarah L. Kieweg, Todd McDonald
  • Patent number: 8865379
    Abstract: Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.
    Type: Grant
    Filed: April 17, 2012
    Date of Patent: October 21, 2014
    Assignee: Inguran, LLC
    Inventors: Johnathan Charles Sharpe, Thomas B. Gilligan, Richard W. Lenz, Juan Moreno
  • Patent number: 8865199
    Abstract: Apparatus and methods are described for generating autologous tissue grafts, the apparatus including a point of care SVF isolation unit that includes a tissue digestion chamber in fluid communication with a lipid separating chamber, whereby SVF cells are isolated without centrifugation; and a cell seeding chamber in fluid communication with the SVF isolation unit, said cell seeding chamber adapted to support a cell scaffold. Methods and materials for cell seeding, including through the provision of micro rough scaffold surfaces, are also provided.
    Type: Grant
    Filed: November 17, 2009
    Date of Patent: October 21, 2014
    Assignees: Ingeneron, Inc., Board of Regents of the University of Texas System
    Inventors: Michael E. Coleman, Eckhard U. Alt, Ron Stubbers
  • Publication number: 20140308741
    Abstract: A method and apparatus for minimizing diagnostic errors due to transposition of biological specimens among subjects provides for independent biometric confirmation that a given specimen is from a given donor. In certain embodiments, a biological specimen confirmation kit comprises a portable and openable case housing components of the kit, at least one biological specimen container adapted to receive a biological testing specimen from a donor, and at least one reference sample device adapted to receive a biological reference specimen from the same donor, such that the testing and reference specimens can later be compared for donor match verification by a reference verification entity.
    Type: Application
    Filed: June 30, 2014
    Publication date: October 16, 2014
    Inventors: Peter M. Knapp, JR., Travis A. Morgan, Theodore R. Schenberg
  • Publication number: 20140308655
    Abstract: The device comprises a loading portion (2a) for loading biological material thereon, and a channel (C) arranged for conducting a warming solution there through and for applying it over said biological material while loaded on said loading portion (2a), for an in-situ warming. The use comprises simultaneously warming and transferring a cryopreserved biological material loaded on a device loading portion, by conducting and applying a warming solution on the biological material loaded on the device loading portion.
    Type: Application
    Filed: May 20, 2011
    Publication date: October 16, 2014
    Applicant: UNIVERSITAT AUTONOMA DE BARCELONA
    Inventors: Teresa Mogas, Roser Morato, Andreu Fontova
  • Patent number: 8859270
    Abstract: The present invention relates to a system for cultivation of algae, extraction of lipids and transesterification of the lipids to obtain biodiesel. The system comprises three sections, that is to say cultivation, extraction and storage and reaction. In the lipid extraction area there is an ultrasonic reactor wherein the external walls of the alga are ruptured together with those of the oil sac to permit the extraction of lipids in the transesterification area there is also an ultrasonic reactor which ruptures the molecules of the fluid which passes therethrough to accelerate the reaction and render it almost immediate.
    Type: Grant
    Filed: September 8, 2008
    Date of Patent: October 14, 2014
    Inventor: Antonio Jose de Jesus de San Juan Bosco Echevarria Parres
  • Patent number: 8859283
    Abstract: The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.
    Type: Grant
    Filed: February 25, 2009
    Date of Patent: October 14, 2014
    Assignee: Genea Limited
    Inventor: Tomas Stojanov
  • Publication number: 20140302482
    Abstract: Embodiments of the present invention include method, system and apparatus for storing fluids (including whole blood or any of the components thereof) to thereby enhance viability of the fluids being stored outside a living body. Embodiments of the present invention include, for example, exposing an electric, magnetic, electromagnetic, field or combinations thereof to one or more storage mediums containing the fluids. Embodiments of the fields exposed to the blood or components thereof thereby causing one or more cells to be repelled from the field to reduce the risk of the one or more cells from sticking together. Embodiments of the claimed invention advantageously extend the viability or shelf life of the blood or components thereof. Embodiments of the present invention can further include a pump for circulating the fluids and a warmer to maintain a constant temperature.
    Type: Application
    Filed: November 23, 2011
    Publication date: October 9, 2014
    Inventors: Dan Dietz, George Daniel O'Clock
  • Publication number: 20140302552
    Abstract: A container for storing a biological sample is disclosed. The container includes a first chamber having a sidewall extending between an open end and a closed end, defining a first chamber interior adapted to receive a sample holder therein. The container also includes a second chamber having a sidewall extending between an open end and a closed end, defining a second chamber interior adapted to subsequently receive the sample holder therein. The second chamber interior is in fluid isolation from the first chamber interior. A removable closure encloses at least one of the open end of the first chamber and the open end of the second chamber while the sample holder is disposed within one of the first chamber interior and the second chamber interior. A first fluid may be disposed within the first chamber interior, and a second different fluid may be disposed within the second chamber interior.
    Type: Application
    Filed: June 19, 2014
    Publication date: October 9, 2014
    Inventors: Bradley M. Wilkinson, C. Mark Newby, Clinton A. Haynes, Robert G. States, III
  • Publication number: 20140302602
    Abstract: A film-shaped tissue storage transport container and storage transport method. The storage transport container includes: an external container; a coupling mechanism which couples a container main body and a lid member of the external container; an internal container which is positioned within the external container; an interposed unit having elasticity and which is sandwiched between the internal container and the lid member; and a storage fluid with which the internal container is filled. A film-shaped tissue is present in a floating state in the storage fluid with which the internal container is filled.
    Type: Application
    Filed: June 20, 2014
    Publication date: October 9, 2014
    Applicant: Terumo Kabushiki Kaisha
    Inventor: Manami KAWASAKI
  • Patent number: 8852435
    Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.
    Type: Grant
    Filed: November 29, 2011
    Date of Patent: October 7, 2014
    Assignee: Therapeutics Proteins International, LLC
    Inventor: Sarfaraz Niazi
  • Publication number: 20140272925
    Abstract: A blood fractionating apparatus that enables aseptic fractionation and storing of fractionated blood components, comprises a centrifuge tube including three chambers separated by constricted sealable passages, an open operative upper end chamber, a closed operative lower end chamber and a middle chamber defined between the upper end chamber and the lower end chamber and connected by means of the sealable passages. A stopper with a flapper valve is provided at the opening of the upper end chamber to control flow of fluid to and from the chambers. This apparatus enables separating the chambers to obtain a hermetically sealed operative lower chamber containing the corpuscle component and a hermetically sealed middle chamber containing the plasma component that can be stored at ambient temperature.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: ChromoLogic LLC
    Inventors: Naresh Menon, Gregory Bearman, Cheryl Tan, Julian Down
  • Publication number: 20140255906
    Abstract: A method and apparatus for storing RBC wherein electromagnetic stimulation such as applying an electrical current or magnetic field to the stored blood extends the viability or shelf life of the stored RBC.
    Type: Application
    Filed: November 23, 2010
    Publication date: September 11, 2014
    Inventor: Dan L. Dietz
  • Patent number: 8820097
    Abstract: A method and system for controlled rate freezing and nucleation of materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling using a laminar and uniform flow of cryogen in proximity to the plurality of vials disposed within the cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as a function of time. The presently disclosed methods to systems also provide nucleation control via temperature quench and depressurized nucleation control.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: September 2, 2014
    Assignee: Praxair, Technology, Inc.
    Inventors: Alan T. Cheng, Yeu-Chuan Simon Ho, Ying Zhou
  • Publication number: 20140234828
    Abstract: The invention relates to a method for separating blood, to a separation container and to a system, wherein different blood fractions—erythrocytes (64), buffy coat (65) and blood plasma (66)—are obtained, wherein blood is introduced into a separation container (1) and is then centrifuged into different superimposed, fluidically connected sections of the separation container, specifically a top section (4) for receiving the blood plasma (66), a middle section (3) for receiving the buffy coat, and a bottom section (2) for receiving the erythrocytes (64). The aim of the invention is to optimize the extraction of buffy coat. Said aim is achieved by means of the method and the separation container, wherein the optimization is geared to obtaining a defined phase boundary, and by means of the system, wherein the optimization is geared to achieving freedom from contamination from the time the blood enters the separation container and obtaining an easy-to-handle freezer container for cryopreservation.
    Type: Application
    Filed: June 19, 2012
    Publication date: August 21, 2014
    Inventor: Walter Pobitschka
  • Publication number: 20140234891
    Abstract: This application discloses cards comprising sintered porous plastic which may be employed in liquid sample collection, storage, transport and/or delivery to an analytical device. Sintered porous plastic materials provide a unique porous structure, an inert substrate, precise liquid holding capability, are quick drying, and easy to cut and handle.
    Type: Application
    Filed: April 16, 2014
    Publication date: August 21, 2014
    Applicant: POREX CORPORATION
    Inventors: Guoqiang Mao, James P. Wingo
  • Publication number: 20140234956
    Abstract: A living cell cryopreservation tool has a living cell holding member having a body part and a living cell holding part and a tubular accommodation member, closed at one end thereof, which is capable of accommodating the living cell holding member. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has heat conductors extended in a longitudinal direction thereof and projected from a distal end thereof. The tubular accommodation member has a heat conductive member accommodated inside a distal portion thereof. When the living cell holding member is inserted into the tubular accommodation member from a distal side of each heat conductor thereof, a distal portion of each heat conductor contacts the heat conductive member of the tubular accommodation member.
    Type: Application
    Filed: October 1, 2012
    Publication date: August 21, 2014
    Inventor: Futoshi Inoue
  • Publication number: 20140220552
    Abstract: Disclosed are devices for collecting blood that contain an anti-coagulant and an additive that delays clotting by inhibiting the contact pathway for thrombin generation. The additive is a coagulation contact pathway inhibitor additive that is at least one of a Factor XI inhibitor, a Factor XII inhibitor, a kallikrein inhibitor and combinations thereof, each in an amount effective to mediate or suppress the contact pathway for thrombin generation. Methods of making and using the devices, and kits containing the devices, are also provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: August 7, 2014
    Applicant: BECTON, DICKINSON AND COMPANY
    Inventors: Keith A. Moskowitz, Frank L. Sinquett
  • Patent number: 8794012
    Abstract: A method and system for controlled rate freezing of biological materials is provided. The presently disclosed system and method provides the ability to rapidly cool the biological materials contained in vials or other containers within a cooling unit via forced convective cooling using a laminar and uniform flow of cryogen in proximity to the plurality of vials disposed within the cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as a function of time.
    Type: Grant
    Filed: November 7, 2008
    Date of Patent: August 5, 2014
    Assignee: Praxair Technology, Inc.
    Inventor: Alan Cheng
  • Patent number: 8794013
    Abstract: A method and system for controlled rate freezing and nucleation of materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling using a laminar and uniform flow of cryogen in proximity to the plurality of vials disposed within the cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as a function of time. The presently disclosed methods to systems also provide nucleation control via temperature quench and depressurized nucleation control.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: August 5, 2014
    Assignee: Praxair Technology, Inc.
    Inventors: Ying Zhou, Theodore H. Gasteyer, III, Nigel J. Grinter, Alan T. Cheng, Yeu-Chuan Simon Ho, Robert R. Sever
  • Publication number: 20140212962
    Abstract: A living cell cryopreservation tool has a body part formed of a cold-resistant material and a living cell holding part formed of the cold-resistant material. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has a plurality of living cell accommodation concave portions formed in a longitudinal direction thereof and a plurality of excess cryopreservation liquid discharge passages communicating with the living cell accommodation concave portions.
    Type: Application
    Filed: October 1, 2012
    Publication date: July 31, 2014
    Applicant: Kitazato BioPharma Co., Ltd.
    Inventor: Futoshi Inoue