Binds A Fungus Or Plant Cell Or Component Or Product Thereof (e.g., Fungus Associated Antigen, Etc.) Patents (Class 435/341)
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Patent number: 10274493Abstract: The invention relates to a method of identifying a specific fungal species in patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the fungal species from the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the fungal species, and specifically identifying the fungal species. The invention also relates to a method of identifying a mycotoxin in patient tissue or body fluid. The method comprises the steps of extracting and recovering the mycotoxin from the patient tissue or body fluid, contacting the mycotoxin with an antibody directed against the mycotoxin, and identifying the myocotoxin. Both of these methods can be used to determine if a patient is at risk for or has developed a disease state related to a fungal infection, and to develop an effective treatment regimen for the patient.Type: GrantFiled: May 2, 2016Date of Patent: April 30, 2019Assignee: Advatect Diagnostics, LLCInventor: Dennis G. Hooper
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Publication number: 20150018532Abstract: The invention relates to antibodies to Aspergillus species and to methods of producing those antibodies. The invention also relates to the use of such antibodies in identifying the presence of the Aspergillus species and to methods of treating an infection with the Aspergillus species.Type: ApplicationFiled: June 6, 2014Publication date: January 15, 2015Applicant: UNIVERSITY OF EXETERInventor: Christopher THORNTON
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Publication number: 20140275492Abstract: The present invention provides for methods of producing human monoclonal antibodies against a wide variety of antigens including bacterial and viral antigens, as well as tumor antigens, and various autoantigens. Also provided are the antibodies themselves, nucleic acids encoding such antibodies, cells producing such antibodies, and methods of using such antibodies for diagnostic assays and passive immunity against disease states such as infection and cancer.Type: ApplicationFiled: March 12, 2014Publication date: September 18, 2014Applicant: MUSC Foundation for Research DevelopmentInventors: Natalie SUTKOWSKI, Semyon Rubinchik
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Publication number: 20140057294Abstract: Hybridoma cell line 10G4 and monoclonal antibody against total aflatoxins produced by the hybridoma cell line 10G4. The hybridoma cell line 10G4 is used to produce the monoclonal antibody that binds specifically total aflatoxin B1, B2, G1 and G2. The titer of the mouse ascites antibody produced by the 10G4 treated mouse is determined through non-competitive enzyme-linked immunosorbent assay and the titer can reach up to 5.12×105. The monoclonal antibody against total aflatoxin B1, B2, G1 and G2 are used for better identification of aflatoxin B1, B2, G1 and G2 with good consistency. The 50% inhibitory concentrations (IC50) of the antibody against aflatoxin B1, B2, G1 and G2 are 2.09 ng/mL, 2.23 ng/mL, 2.19 ng/mL and 3.21 ng/mL respectively. The range of cross reaction rate for aflatoxin B1, B2, G1 and G2 is about 65.2%-100%. The antibody is used for quantitative measurement of total aflatoxin B1, B2, G1 and G2.Type: ApplicationFiled: January 17, 2013Publication date: February 27, 2014Inventors: Peiwu Li, Xin Li, Qi Zhang, Xiaoxia Ding, Wen Zhang, Ran Li, Zhaowei Zhang
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Publication number: 20120263719Abstract: The present invention provides monoclonal antibodies specific for members of the Aspergillus genus and uses thereof for increasing the survival rate of a subject infected with an Aspergillus species. The present invention also provides conjugates of alliinase with monoclonal antibodies specific for members of the Aspergillus genus that preserve the ability of the antibody to recognize the fungus as well as the activity of the enzyme to produce cytotoxic molecules of allicin from the substrate alliin. The invention further provides pharmaceutical compositions comprising the conjugates of the mAbs of the invention and alliinase and uses thereof in combination with the substrate alliin in the treatment of diseases cause by Aspergillus species, in particular invasive aspergillosis.Type: ApplicationFiled: October 21, 2010Publication date: October 18, 2012Applicant: YEDA RESEARCH AND DEVELOPMENT CO., LTD.Inventors: David Mirelman, Aharon Rabinkov, Elena Appel
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Publication number: 20120064093Abstract: The invention relates to antibodies to Aspergillus species and to methods of producing those antibodies. The invention also relates to the use of such antibodies in identifying the presence of the Aspergillus species and to methods of treating an infection with the Aspergillus species.Type: ApplicationFiled: January 18, 2010Publication date: March 15, 2012Inventor: Christopher Thornton
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Publication number: 20110318758Abstract: The present invention provides for methods of producing human monoclonal antibodies against a wide variety of antigens including bacterial and viral antigens, as well as tumor antigens, and various autoantigens. Also provided are the antibodies themselves, nucleic acids encoding such antibodies, cells producing such antibodies, and methods of using such antibodies for diagnostic assays and passive immunity against disease states such as infection and cancer.Type: ApplicationFiled: August 4, 2008Publication date: December 29, 2011Applicant: MUSC FOUNDATION FOR RESEARCH DEVELOPMENTInventors: Natalie Sutkowski, Semyon Rubinchik
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Patent number: 8049001Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof.Type: GrantFiled: May 13, 2008Date of Patent: November 1, 2011Assignee: Canon Kabushiki KaishaInventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
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Patent number: 8039607Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof.Type: GrantFiled: May 13, 2008Date of Patent: October 18, 2011Assignee: Canon Kabushiki KaishaInventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
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Patent number: 8034918Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof.Type: GrantFiled: May 13, 2008Date of Patent: October 11, 2011Assignee: Canon Kabushiki KaishaInventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
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Patent number: 8034919Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof.Type: GrantFiled: May 13, 2008Date of Patent: October 11, 2011Assignee: Canon Kabushiki KaishaInventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
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Patent number: 7960519Abstract: A method of treating biologic material with a composition containing at least one fatty acid derivative and/or at least one fatty alcohol, a buffer, and a salt to result in protein partitioning into an aqueous phase and an organic phase. The protein may be a membrane protein. The cells may be plant cells and/or animal cells. The proteins can be thereafter be used for downstream applications.Type: GrantFiled: May 30, 2006Date of Patent: June 14, 2011Assignee: Pierce Biotechnology, Inc.Inventors: Valerie J. O'Sullivan, Edward J. Conklin
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Patent number: 7897387Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: April 1, 2008Date of Patent: March 1, 2011Assignee: Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7893219Abstract: Anti-?-1,3-glucan antibodies have been found to be protective against systemic fungal infection with Candida albicans. The present invention provides monoclonal antibodies that bind to ?-1,3-glucan, hybridoma cell lines producing the antibodies, compositions comprising the antibodies and methods of using such antibodies for treatment of microbial infections, particularly against Candida albicans and Aspergillus fumigatis infections. The antibodies of the present invention are not specific for ?-1,6-glucan.Type: GrantFiled: September 14, 2005Date of Patent: February 22, 2011Assignee: Novartis Vaccines & Diagnostics, Inc.Inventors: Antonio Cassone, Antonella Torosantucci
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Patent number: 7776553Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: September 18, 2006Date of Patent: August 17, 2010Assignee: Presidents and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Patent number: 7166706Abstract: The invention concerns the use of ketol-acid reductoisomerase inhibitors for treating fungal diseases affecting crops. The invention concerns methods for treating crops against fungal diseases comprising applying a ketol-acid reductoisomerase inhibitor. The invention also concerns methods for identifying novel fungicidal compounds comprising a step which consists in identifying ketol-acid reductoisomerase inhibitors.Type: GrantFiled: March 10, 2004Date of Patent: January 23, 2007Assignee: Bayer Cropscience S.A.Inventors: Renaud Dumas, Marc-Henri Lebrun, Jean-Luc Zundel, Géraldine Effantin, Valérie Morin
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Patent number: 6815169Abstract: The invention provides isolated nucleic acid and amino acid sequences of TL-&ggr;, antibodies to TL-&ggr;, methods of screening for TL-&ggr; modulators using biologically active TL-&ggr;, and kits for screening for TL-&ggr; modulators.Type: GrantFiled: November 28, 2000Date of Patent: November 9, 2004Assignee: The Regents of the University of CaliforniaInventors: Roman Sakowicz, Lawrence S. B. Goldstein
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Patent number: 6783986Abstract: Proteins having the activity of saccharose phosphate synthetase (SPS) and a process for obtaining the same.Type: GrantFiled: April 26, 1995Date of Patent: August 31, 2004Assignee: Aventis Crop Sciences GmbHInventors: Charles Van Assche, Danielle Lando, Jean Michel Bruneau, Toni Alois Voelker, Monica Gervais
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Patent number: 6482601Abstract: A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.Type: GrantFiled: September 11, 2000Date of Patent: November 19, 2002Assignee: Diachemix LLCInventors: Mohammad Sarwar Nasir, Michael E. Jolley
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Patent number: 6329197Abstract: This invention provides an isolated nucleic acid encoding a mammalian galanin receptor, an isolated galanin receptor protein, vectors comprising isolated nucleic acid encoding a mammalian galanin receptor, cells comprising such vectors, antibodies directed to a mammalian galanin receptor, nucleic acid probes useful for detecting nucleic acid encoding a mammalian galanin receptor, antisense oligonucleotides complementary to unique sequences of nucleic acid encoding a mammalian galanin receptor, nonhuman transgenic animals which express DNA encoding a normal or a mutant mammalian galanin receptor, as well as methods of determining binding of compounds to mammalian galanin receptors.Type: GrantFiled: July 23, 1997Date of Patent: December 11, 2001Assignee: Synaptic Pharmaceutical CorporationInventors: Jonathan A. Bard, Beth Borowsky, Kelli E. Smith
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Patent number: 5616492Abstract: Monoclonal antibodies and a method capable of detecting BBI metabolites in a sample of body fluid or tissue are provided.Type: GrantFiled: June 2, 1995Date of Patent: April 1, 1997Assignees: Trustees of the University of Pennsylvania, University of RochesterInventors: Ann R. Kennedy, Cameron J. Koch, Edith M. Lord, Xingsheng Wan
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Patent number: 5614408Abstract: A hybridoma cell lines is described which produces and secretes a monoclonal antibody which selectively binds to the glycoalkaloids of potatoes, tomatoes, and eggplants, as well as their corresponding aglycones. Glycoalkaloids of potatoes, tomatoes, and/or eggplants in biological samples may be detected and quantified by contacting the sample with the antibodies to form a glycoalkaloid/antibody immunocomplex when the glycoalkaloids are present, which immunocomplex may then be detected. The monoclonal antibody may also be incorporated into kits for the detection and quantification of glycoalkaloids in plants, foods, and body tissues and fluids.Type: GrantFiled: October 18, 1995Date of Patent: March 25, 1997Assignee: The United States of America as represented by the Secretary of AgriculturalInventors: Larry H. Stanker, Carol K. Holtzapple, Mendel Friedman