Blood Or Lymphatic Origin Or Derivative Patents (Class 435/355)
  • Patent number: 9803164
    Abstract: Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: October 31, 2017
    Assignee: NEW YORK BLOOD CENTER, INC.
    Inventor: Beau W. Mitchell
  • Patent number: 9034922
    Abstract: A composition for maintaining a function of platelets, the composition comprising, as an active ingredient, a compound represented by the following general formula (I) or a salt thereof, or a solvate thereof: wherein X represents a phenylene group; Y represents any one of a hydrogen atom and —(CH2)mR1; wherein m represents an integer of any one of 0 to 4; and R1 is any one of —NR5COR2, —NR5SO2R2, and —NR3R4; wherein R2 represents any one of a C1 to C6 alkyl group, an aryl group, a C1 to C6 alkoxy group, and the like; R3 and R4 represent a C1 to C6 alkyl group or the like; and R5 represents any one of a hydrogen atom, a C1 to C6 alkyl group, and the like; and Z represents any one of a hydrogen atom and a C1 to C6 alkyl group.
    Type: Grant
    Filed: September 16, 2011
    Date of Patent: May 19, 2015
    Assignees: THE UNIVERSITY OF TOKYO, KAKEN PHARMACEUTICAL CO., LTD.
    Inventors: Koji Eto, Ryoko Ohnishi, Hiromitsu Nakauchi, Takahiko Murata
  • Patent number: 9034649
    Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.
    Type: Grant
    Filed: June 11, 2010
    Date of Patent: May 19, 2015
    Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTD
    Inventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
  • Publication number: 20150133379
    Abstract: The present invention relates to a fusion protein comprising a skin-penetrating peptide, a polynucleotide encoding the fusion protein, an expression vector comprising the polynucleotide, a transformant comprising the expression vector, a method for preparing the fusion protein, a cosmetic composition for improving skin conditions, which comprises the fusion protein, and a pharmaceutical composition for external skin use, which comprises the fusion protein. The fusion protein of the invention comprises a skin-penetrating peptide bound to a physiologically active protein. The fusion protein significantly enhances the skin penetration and skin retention of the physiologically active protein while maintaining or enhancing the ability of the physiologically active protein to synthesize a material showing physiologically active effects. Thus, it can be widely used as an active ingredient in functional cosmetic compositions and pharmaceutical compositions for external skin use.
    Type: Application
    Filed: April 30, 2014
    Publication date: May 14, 2015
    Inventors: Seol Hoon Lee, Sang Hwa Lee, Nae Gyu Kang, Eu Gene Hur
  • Publication number: 20150113669
    Abstract: The invention discloses methods for the generation of chimaeric human—non-human antibodies and chimaeric antibody chains, antibodies and antibody chains so produced, and derivatives thereof including fully humanised antibodies; compositions comprising said antibodies, antibody chains and derivatives, as well as cells, non-human mammals and vectors, suitable for use in said methods.
    Type: Application
    Filed: September 25, 2014
    Publication date: April 23, 2015
    Inventors: Allan Bradley, E-Chiang Lee, Qi Liang, Wei Wang
  • Publication number: 20150099705
    Abstract: The present invention relates to Protoxin-II variants, polynucleotides encoding them, and methods of making and using the foregoing.
    Type: Application
    Filed: October 3, 2014
    Publication date: April 9, 2015
    Inventors: Mack FLINSPACH, Alan Wickenden, Ross Fellows, Robert Neff, Yi Liu, Rebecca Hagan, Qinghao Xu
  • Publication number: 20150093417
    Abstract: The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the CD45 gene.
    Type: Application
    Filed: December 2, 2014
    Publication date: April 2, 2015
    Inventors: Antonin de Fougerolles, Pamela Tan, Anna Borodovsky, Tatiana Novobrantseva, Sina Bavari, Kelly Lynn Warfield
  • Publication number: 20150089680
    Abstract: Genetically modified mice are provided that express human ? variable (hV?) sequences, including mice that express hV? sequences from an endogenous mouse ? light chain locus, mice that express hV? sequences from an endogenous mouse ? light chain locus, and mice that express hV? sequences from a transgene or an episome wherein the hV? sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human ? variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human ? variable sequences, including human antibodies, are provided.
    Type: Application
    Filed: December 5, 2014
    Publication date: March 26, 2015
    Inventors: Lynn Macdonald, Sean Stevens, Cagan Gurer, Andrew J. Murphy, Karolina A. Meagher
  • Publication number: 20150087065
    Abstract: The present invention is a method and kits for generating a homogenous population of hematopoietic progenitor cells capable of differentiating into a hematopoietic cell lineage. Whereas the combination of Homeobox-B8 protein and FMS-like tyrosine kinase 3 ligand generate cells with the potential to differentiate into different myeloid and lymphoid cell types, Homeobox-A7 protein and erythropoietin generate cells with the potential to differentiate into erythropoietic or thrombopoietic cell types.
    Type: Application
    Filed: April 18, 2013
    Publication date: March 26, 2015
    Inventor: Hans Haecker
  • Publication number: 20150024412
    Abstract: Genetically modified non-human animals and methods and compositions for making and using them are provided, wherein the genetic modification comprises a deletion of the endogenous low affinity Fc?R locus, and wherein the mouse is capable of expressing a functional FcR?-chain. Genetically modified mice are described, including mice that express low affinity human Fc?R genes from the endogenous Fc?R locus, and wherein the mice comprise a functional FcR?-chain. Genetically modified mice that express up to five low affinity human Fc?R genes on accessory cells of the host immune system are provided.
    Type: Application
    Filed: October 7, 2014
    Publication date: January 22, 2015
    Inventors: Lynn Macdonald, Naxin Tu, Cagan Gurer, Li-Hsien Wang, Sean Stevens, Andrew J. Murphy
  • Publication number: 20140363845
    Abstract: The disclosure provides compositions and methods for high density cell culture and banking of cholesterol auxotrophic cells.
    Type: Application
    Filed: May 15, 2014
    Publication date: December 11, 2014
    Applicant: Biogen Idec MA Inc.
    Inventors: Marty Sinacore, Yiwen Tao, Thomas Ryll, Helena Yusuf-Makagiansar
  • Patent number: 8865425
    Abstract: The present invention relates to a reagent for the measurement of FDP comprising a carrier sensitized with at least two monoclonal antibodies selected from three monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent kit comprising the reagent and a method for measurement of FDP using the reagent or reagent kit.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: October 21, 2014
    Assignee: Sysmex Corporation
    Inventors: Katsushi Kobayashi, Masumi Murakami, Mayumi Sugimoto
  • Patent number: 8865424
    Abstract: The present invention relates to an anti-FDP monoclonal antibody selected from the first, second and third monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent and reagent kit for the measurement of FDP and a method for measurement of FDP using the anti-FDP monoclonal antibodies.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: October 21, 2014
    Assignee: Sysmex Corporation
    Inventors: Naoya Okitsu, Katsushi Kobayashi, Noriaki Nakajima, Masumi Murakami, Kouji Sakaguchi, Ayumi Asaeda, Mayumi Sugimoto
  • Patent number: 8846373
    Abstract: The present invention relates to new methods to promote sialylation of glycoconjugates, including recombinant glycoproteins, in glycoconjugate production systems. The invention relates to methods to promote efficient glycoconjugate sialylation in recombinant expression systems, by providing simpler and more economical ways to produce large intracellular pools of sialic acid precursors. The invention is directed to nucleic acids, vectors, and cells harboring vectors comprising nucleic acids encoding enzymes involved in the synthesis of sialic acid precursors, and cells harboring these nucleic acids in combination with nucleic acids encoding glycosyltransferases, including sialyltransferases, to facilitate the production of humanized recombinant glycoproteins in bacterial, fungal, plant, and animal cell expression systems.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: September 30, 2014
    Assignee: The University of Wyoming
    Inventors: Christoph Geisler, Donald Jarvis
  • Patent number: 8809051
    Abstract: The present invention relates to transgenic non-human animals that are engineered to contain human immunoglobulin gene loci. In particular, animals in accordance with the invention possess human Ig loci that include plural variable (VH and V?) gene regions. Advantageously, the inclusion of plural variable region genes enhances the specificity and diversity of human antibodies produced by the animal. Further, the inclusion of such regions enhances and reconstitutes B-cell development to the animals, such that the animals possess abundant mature B-cells secreting extremely high affinity antibodies.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: August 19, 2014
    Assignee: Amgen Fremont Inc.
    Inventors: Aya Jakobovits, Raju Kucherlapati, Susan Klapholz, Michael J Mendez, Larry Green
  • Publication number: 20140199319
    Abstract: Disclosed herein are methods for improving the efficiency of the production of hybridomas, e.g., through enrichment of IgG expressing B cells. Also disclosed are hybridoma compositions produced using these methods as well as methods for producing antibodies with the hybridomas.
    Type: Application
    Filed: December 13, 2013
    Publication date: July 17, 2014
    Applicant: AbbVie, Inc.
    Inventors: Jane SEAGAL, Eve H. BARLOW, Chung-Ming HSIEH, Jeffrey Yen PAN, Shawn M. JENNINGS, Mary R. LEDDY, Archana B. THAKUR
  • Publication number: 20140193361
    Abstract: Obesity is associated with a state of chronic low-grade inflammation and the present invention establishes that adipose-resident natural killer T (NKT) cells attenuate inflammation in adipose tissue and improves systemic glucose homeostasis in mice at different stages of obesity. Accordingly, the present invention provides methods of treating type-2 diabetes or those at risk for type-2 diabetes using activators of adipose-resident NKT cells. Such activators include particular glycolipids (e.g., a-galactosyl-ceramide and its analogs other than sulfated analogs) and cytokines that promote M2 macrophage polarization. The invention also includes methods to screen for activators of adipose-resident NKT cells.
    Type: Application
    Filed: August 30, 2012
    Publication date: July 10, 2014
    Applicant: CORNELL UNIVERSITY
    Inventors: Ling Qi, Yewei JI
  • Publication number: 20140155290
    Abstract: Improved host cells and culture methods involving overexpression of MAN1C1 activity to improve protein production are provided.
    Type: Application
    Filed: February 6, 2014
    Publication date: June 5, 2014
    Applicant: AMGEN INC.
    Inventor: Christopher Kenyon Crowell
  • Publication number: 20140134722
    Abstract: The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha-kinases are provided.
    Type: Application
    Filed: June 17, 2010
    Publication date: May 15, 2014
    Inventor: Alexey Ryazanov
  • Patent number: 8679838
    Abstract: It is intended to provide a serum which contains a large amount of growth factors capable of efficiently promoting the growth of stem cells. A human serum for cell culture which shows a residual ratio of platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%.
    Type: Grant
    Filed: February 12, 2010
    Date of Patent: March 25, 2014
    Assignee: JMS Co., Ltd.
    Inventors: Koji Suzuki, Seishin Tanaka
  • Publication number: 20140065157
    Abstract: Gene-modified, inflammation-specific monocytes that comprise a 1-alpha-hydroxylase gene, where the 1-alpha-hydroxylase gene is expressed to produce functional 1-alpha-hydroxylase enzyme when the monocytes transdifferentiate into gene-modified, inflammation-specific macrophages. Gene-modified, inflammation-specific macrophages that comprise a 1-alpha-hydroxylase gene. A method for treating one or more than one inflammation-related condition or disease, the method comprising administering gene-modified, inflammation-specific monocytes that comprise a 1-alpha-hydroxylase gene, where the 1-alpha-hydroxylase gene is expressed to produce functional 1-alpha-hydroxylase enzyme when the monocytes transdifferentiate into gene-modified, inflammation-specific macrophages.
    Type: Application
    Filed: November 8, 2013
    Publication date: March 6, 2014
    Applicant: LOMA LINDA UNIVERSITY
    Inventors: David J. Baylink, Kin-Hing William Lau, Xuezhong Qin
  • Publication number: 20140047572
    Abstract: Disclosed are transgenic non-human mammals, which useful for the screening of thrombopoietin mimetics, thrombopoietin receptor agonists, or thrombopoietin receptor antagonists active on the human thrombopoietin receptor. The transgenic non-human mammal has a genome that comprises a stably integrated transgene construct comprising a polynucleotide sequence encoding a humanized thrombopoietin receptor wherein said transgenic non-human mammal has a baseline blood platelet count corresponding to a physiological blood platelet count of a matched non-transgenic non-human mammal. The chimeric thrombopoietin receptor comprises either the transmembrane domain of a human thrombopoietin receptor or both the extracellular and transmembrane domains of a human thrombopoietin receptor operably coupled to a cytoplasmic domain of a non-human thrombopoietin receptor.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 13, 2014
    Applicant: University of Rochester
    Inventors: Yuhchyau CHEN, J.H. David WU, Jane L. LIESVELD, Lin GAN
  • Patent number: 8642835
    Abstract: Mice are provided that comprise a reduction or deletion of ADAM6 activity from an endogenous ADAM6 locus, or that lack an endogenous locus encoding a mouse ADAM6 protein, wherein the mice comprise a sequence encoding an ADAM6 or ortholog or homolog or fragment thereof that is functional in a male mouse. In one embodiment, the sequence is an ectopic ADAM6 sequence or a sequence that confers upon a male mouse the ability to generate offspring by mating. Mice and cells with genetically modified immunoglobulin heavy chain loci that comprise an ectopic nucleotide sequence encoding a mouse ADAM6 or functional fragment or homolog or ortholog thereof are also provided.
    Type: Grant
    Filed: February 24, 2012
    Date of Patent: February 4, 2014
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Lynn MacDonald, Sean Stevens, Andrew J. Murphy
  • Patent number: 8637307
    Abstract: Provided are a system and methods for selectively inducing expansion of a population of T cells in the absence of exogenous growth factors, such as lymphokines, and accessory cells for research purposes. The cell based expansion system and methods permit the long-term growth of CTLs, preferably human CTLs. In addition, T cell proliferation can be induced without the need for antigen, thus providing an expanded T cell population that is polyclonal with respect to antigen reactivity. Further provided are methods for using the system and methods to screen and identify antigens related to specific diseases or conditions, tumors, autoimmune disorders, or an infectious disease or pathogen, and to identify target molecule for research purposes, or for developing a vaccine based thereon.
    Type: Grant
    Filed: May 10, 2010
    Date of Patent: January 28, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Carl H. June, James L. Riley, Marcela Maus, Anna Thomas, Robert Vonderheide
  • Publication number: 20130337506
    Abstract: The present invention is related to a cell culture medium for the expression of a protein, which medium comprises a PAM inhibitor, or a physiological equivalent thereof, and to a cell culture process for the expression of a protein, in which process a PAM inhibitor, or a physiological equivalent thereof, is used (FIG. 1).
    Type: Application
    Filed: November 9, 2011
    Publication date: December 19, 2013
    Applicant: SANDOZ GMBH
    Inventors: Corinna Sonderegger, Julia Schmutzhard, Christine Heel, Thomas Stangler
  • Publication number: 20130316354
    Abstract: Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided.
    Type: Application
    Filed: July 22, 2013
    Publication date: November 28, 2013
    Applicant: Merck Sharp & Dohme Corp.
    Inventor: J. Fernando Bazan
  • Publication number: 20130209499
    Abstract: Provided herein are polypeptides comprising portions of the influenza virus hemagglutinin, compositions comprising such polypeptides that can be used as immunogens in vaccines and methods of their use to generate an immune response against multiple influenza subtypes in a subject.
    Type: Application
    Filed: February 18, 2011
    Publication date: August 15, 2013
    Applicant: Mount Sinai School of Medicine
    Inventors: Adolfo Garcia-Sastre, Peter Palese, Gens S. Tan, Taia T. Wang
  • Patent number: 8486693
    Abstract: Modified antigen presenting cells provided herein have improved lifespan and immunogenicity compared to unmodified antigen presenting cells, and are useful for immunotherapy. The modified antigen presenting cells express an altered protein kinase, referred to herein as “Akt.” The altered Akt associates with the cell membrane with greater frequency than unaltered Akt, and is referred to herein as “membrane-targeted Akt.
    Type: Grant
    Filed: September 19, 2012
    Date of Patent: July 16, 2013
    Assignee: Bellicum Pharmaceuticals, Inc.
    Inventors: Dongsu Park, David Spencer, Natalia Lapteva
  • Publication number: 20130177987
    Abstract: Disclosed are methods for producing class-switched, affinity-matured antibodies which include enriching an immunized cell population for GL7-positive cells and activating the enriched cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells or to improve the quality of molecularly cloned Ig heavy and light chains.
    Type: Application
    Filed: June 25, 2012
    Publication date: July 11, 2013
    Applicant: NEOCLONE BIOTECHNOLOGY INTERNATIONAL, LLC
    Inventors: Brian Schram, Rachel Kravitz
  • Publication number: 20130177900
    Abstract: A composition for maintaining a function of platelets, the composition comprising, as an active ingredient, a compound represented by the following general formula (I) or a salt thereof, or a solvate thereof: wherein X represents a phenylene group; Y represents any one of a hydrogen atom and —(CH2)mR1; wherein m represents an integer of any one of 0 to 4; and R1 is any one of —NR5COR2, —NR5SO2R2, and —NR3R4; wherein R2 represents any one of a C1 to C6 alkyl group, an aryl group, a C1 to C6 alkoxy group, and the like; R3 and R4 represent a C1 to C6 alkyl group or the like; and R5 represents any one of a hydrogen atom, a C1 to C6 alkyl group, and the like; and Z represents any one of a hydrogen atom and a C1 to C6 alkyl group.
    Type: Application
    Filed: September 16, 2011
    Publication date: July 11, 2013
    Applicants: KAKEN PHARMACEUTICAL CO., LTD., THE UNIVERSITY OF TOKYO
    Inventors: Koji Eto, Ryoko Ohnishi, Hiromitsu Nakauchi, Takahiko Murata
  • Patent number: 8476071
    Abstract: The present invention relates to methods for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: July 2, 2013
    Assignee: Stowers Institute for Medical Research
    Inventors: John M. Perry, Linheng Li, Justin C. Grindley
  • Publication number: 20130158095
    Abstract: The present inventors found that a fusion gene present in some cancer patients is an oncogene. The present invention relates to a polypeptide as a novel fusion protein, a polynucleotide encoding the polypeptide, a vector comprising the polynucleotide, a transformed cell comprising the vector, a method for detecting the fusion protein or polynucleotide, a method for screening a therapeutic agent for cancer, and a method for treating cancer that is shown to be positive for the fusion gene. Further, the present invention relates kit, primer set, and probe useful in the detection of cancer that is shown to be positive for the fusion gene.
    Type: Application
    Filed: May 10, 2012
    Publication date: June 20, 2013
    Applicants: CureGene K.K., Astellas Pharma Inc.
    Inventors: Hiroyuki Mano, Sadao Kuromitsu, Nobuaki Shindo, Takatoshi Soga, Takashi Furutani
  • Publication number: 20130142830
    Abstract: This invention discloses an unexpected discovery that plasmacytoid dendritic cells (pDCs) may be segregated into immunogenic or tolerogenic species based on novel biomarkers discovered herein. Exemplary biomarkers include CD8?+?+, CD8?+??, CD8????, C1q, and IL-9R. For example, pDCs with CD8?+?+, CD8?+?? are tolerogenic and CD8???? is immunogenic. Also disclosed are isolated pDCs, compositions comprising the pDCs, methods for isolating the pDCs, methods for treating immune-hyper-reactivity, such as airway hyper-reactivity, food allergy, asthma, and autoimmune disorders, by using compositions containing tolerogenic antigen presenting cells, preferably pDCs disclosed herein. Also disclosed are methods for identifying tolerogenic antigen presenting cells by using one or more novel biomarkers disclosed herein, including RALDH expression, CD8?, CD8?C1qa, C1qc, and IL-9R. Also disclosed are methods for inducing Treg cells by using the pDCs disclosed herein.
    Type: Application
    Filed: May 11, 2012
    Publication date: June 6, 2013
    Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA
    Inventors: Omid AKBARI, Vincent LOMBARDI
  • Publication number: 20130109089
    Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.
    Type: Application
    Filed: October 5, 2012
    Publication date: May 2, 2013
    Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
    Inventor: Whitehead Institute for Biomedical Research
  • Publication number: 20130109090
    Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.
    Type: Application
    Filed: October 5, 2012
    Publication date: May 2, 2013
    Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
    Inventor: Whitehead Institute for Biomedical Research
  • Publication number: 20130064859
    Abstract: A method of increasing the percentage of mouse and human Tregs is provided comprising providing B.fragilis or PSA and vitamin D or vitamin D metabolite to said mouse or human. Also, a method for treating inflammatory bowel diseases in an individual is presented where the individual is given a combination of vitamin D and B. fragilis or PSA. Such a treatment may be particularly effective in individuals who are either Vitamin D insufficient or deficient.
    Type: Application
    Filed: January 28, 2012
    Publication date: March 14, 2013
    Inventors: Sarkis K. Mazmanian, Martin Hewison, Yue Shen, Venu Lagishetty
  • Publication number: 20130052728
    Abstract: The invention provides compositions for making erythroid progenitor cells that comprise in vitro-activated bone marrow mesenchymal stem cells and embryoid bodies (EBs) or pluripotent stem cells, and methods for making and using them, including ameliorating (e.g., preventing or treating) anemia and/or stimulating erythropoiesis. In one embodiment, the invention provides methods of increasing propensity of committed stem cell differentiation towards the erythroid lineage.
    Type: Application
    Filed: November 30, 2010
    Publication date: February 28, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventor: Ewa Carrier
  • Publication number: 20130029413
    Abstract: The present invention relates to new methods to promote sialylation of glycoconjugates, including recombinant glycoproteins, in glycoconjugate production systems. The invention relates to methods to promote efficient glycoconjugate sialylation in recombinant expression systems, by providing simpler and more economical ways to produce large intracellular pools of sialic acid precursors. The invention is directed to nucleic acids, vectors, and cells harboring vectors comprising nucleic acids encoding enzymes involved in the synthesis of sialic acid precursors, and cells harboring these nucleic acids in combination with nucleic acids encoding glycosyltransferases, including sialyltransferases, to facilitate the production of humanized recombinant glycoproteins in bacterial, fungal, plant, and animal cell expression systems.
    Type: Application
    Filed: July 27, 2012
    Publication date: January 31, 2013
    Applicant: UNIVERSITY OF WYOMING
    Inventors: Christoph Geisler, Donald Jarvis
  • Publication number: 20130011919
    Abstract: The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha-kinases are provided.
    Type: Application
    Filed: June 17, 2010
    Publication date: January 10, 2013
    Inventor: Alexey Ryazanov
  • Publication number: 20120315643
    Abstract: Provided are alternative splicing constructs and methods for their use. In particular, CD44 based alternative splicing constructs are provided that include CD44 exon 5. These alternative splicing constructs are useful in high-throughput assays for testing the effects of compounds on splicing and for achieving targeted cell death.
    Type: Application
    Filed: May 31, 2012
    Publication date: December 13, 2012
    Inventors: Dayle A. Daines, Robert J. McKallip
  • Publication number: 20120283120
    Abstract: The present invention provides a novel variant androgen receptor (ARaiv) lacking ligand binding domain, a nucleic acid encoding the same and use thereof. That is, the present invention provides a method of screening for a substance for the prophylaxis or treatment of cancer, which includes contacting an ARaiv protein or ARaiv-producing cell with a test compound, measuring the activity of ARaiv (e.g., transcription regulating action of androgen responsive gene) or expression level thereof, and selecting a compound that suppresses the activity or expression level.
    Type: Application
    Filed: September 28, 2010
    Publication date: November 8, 2012
    Applicant: Takeda Pharmaceutical Company Limited
    Inventors: Tatsuya Watanabe, Kazuhide Nakayama, Daisuke Nakata, Masami Kusaka
  • Publication number: 20120269840
    Abstract: The present invention relates to the efficient expression of HIV polypeptides in a variety of cell types, including, but not limited to, mammalian, insect, and plant cells. Synthetic expression cassettes encoding the HIV Gag-containing polypeptides are described, as are uses of the expression cassettes in applications including DNA immunization, generation of packaging cell lines, and production of Env-, tat- or Gag-containing proteins. The invention provides methods of producing Virus-Like Particles (VLPs), as well as, uses of the VLPs including, but not limited to, vehicles for the presentation of antigens and stimulation of immune response in subjects to whom the VLPs are administered.
    Type: Application
    Filed: April 27, 2012
    Publication date: October 25, 2012
    Applicant: NOVARTIS VACCINES AND DIAGNOSTICS. INC.
    Inventors: Susan W. BARNETT, Jan ZUR MEGEDE, Indresh SRIVASTAVA, Ying LIAN, Karin HARTOG, Hong LIU, Catherine GREER, Mark SELBY, Christopher WALKER
  • Publication number: 20120252699
    Abstract: The present invention relates to novel fluorophores and their use in combination with novel nucleic acid molecules, called aptamers, that bind specifically to the fluorophore and thereby enhance the fluorescence signal of the fluorophore upon exposure to radiation of suitable wavelength. Molecular complexes formed between the novel fluorophores, novel nucleic acid molecules, and their target molecules are described, and the use of multivalent aptamer constructs as fluorescent sensors for target molecules of interest are also described.
    Type: Application
    Filed: February 18, 2010
    Publication date: October 4, 2012
    Applicant: CORNELL UNIVERSITY
    Inventors: Samie R. Jaffrey, Jeremy S. Paige
  • Patent number: 8278097
    Abstract: The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a coating layer formed from a compound having a particular PC-like group on a vessel surface defining a region for floating culture of ES cells, and (B) floating culturing ES cells in the vessel to form embryoid bodies.
    Type: Grant
    Filed: June 8, 2009
    Date of Patent: October 2, 2012
    Assignee: NOF Corporation
    Inventors: Hiroshi Kurosawa, Shujiro Sakaki
  • Publication number: 20120237496
    Abstract: The present invention relates to polypeptides comprising protease variants of wild type human neprilysin having an altered specificity and/or activity. In particular the present invention relates to polypeptides comprising protease variants derived from human neprilysin having an increased specificity and/or activity against certain substrates, in particular against amyloid beta.
    Type: Application
    Filed: June 21, 2010
    Publication date: September 20, 2012
    Inventors: Joerg Birkenfeld, Andrea Eicker, Per-Ola Freskgard, Claudia Gotzberger-Schad, Joanna Grudzinska, Ulrich Haupts, Josi Innig, Christoph Mahlert, Andreas Scheidig, Michael Strerath, Jan Tebbe, Johan Per-Wallin, Nina Wobst, Carl Innes Webster, Lutz Jermutus
  • Patent number: 8263400
    Abstract: A method for the expansion of adult stem cells from blood, particularly but not only peripheral blood, involves removing adult stem cells from blood of a mammal, immediately expanding the stem cells via in-vitro treatment with MCSF (Macrophage Colony Stimulating Factor) at a concentration of about 8-15 nM, and purifying the expanded stem cells. Compositions and methods of using the expanded adult stem cells are also described.
    Type: Grant
    Filed: March 20, 2009
    Date of Patent: September 11, 2012
    Assignee: Thankstem Srl
    Inventors: Alessandra Gambacurta, Marco Polettini
  • Patent number: 8263403
    Abstract: The present invention relates to methods for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population.
    Type: Grant
    Filed: April 23, 2008
    Date of Patent: September 11, 2012
    Assignee: Stowers Institute for Medical Research
    Inventors: John M. Perry, Linheng Li, Justin C. Grindley
  • Publication number: 20120202751
    Abstract: Transgenic mammals, cells derived from the animals, and methods of using these to monitor the endoplasmic reticulum (ER) stress response are provided. In some embodiments, the methods allow for monitoring the ER stress response in real time. Some of the methods allow non-invasive in vivo visualization of ER stress response. Also provided are methods of screening molecules and/or treatment conditions for the ability to modulate the ER stress response, methods of treating diseases characterized by ER stress response activity, and methods of detecting the toxicity or therapeutic ratio of molecules that modulate the ER stress response.
    Type: Application
    Filed: August 10, 2010
    Publication date: August 9, 2012
    Applicant: The Board of trustees of the Leland Stanford Junior University
    Inventors: Albert C. Koong, Michael T. Spiotto, George P. Yang
  • Patent number: 8232100
    Abstract: Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified.
    Type: Grant
    Filed: July 21, 2010
    Date of Patent: July 31, 2012
    Assignee: Isis Innovation Limited
    Inventors: Herman Waldmann, Paul J. Fairchild, Richard Gardner, Frances Brook
  • Publication number: 20120185956
    Abstract: A viable global NaV1.7?/? knockout mouse is disclosed, and a breeding colony of global NaV1.7?/? knockout mice. Also disclosed are an isolated mouse gamete that does not encode a functional NaV1.7?/?, produced by the NaV1.7?/? knockout mouse; an isolated NaV1.7?/? mouse cell, or a progeny cell thereof, isolated from the NaV1.7?/? knockout mouse; and a primary cell culture or a secondary cell line and a tissue or organ explant or culture thereof derived from the NaV1.7?/? knockout mouse. Disclosed also are a hybridoma, wherein the hybridoma was originally formed from the fusion of the isolated NaV1.7?/? mouse cell mouse cell and a myeloma cell, and a method of making an antibody. Also disclosed are assays useful for screening prospective NaV1.7 inhibitors and dose ranging a test NaV17 inhibitor compound, which were validated using the NaV1.7?/? knockout mouse.
    Type: Application
    Filed: January 18, 2012
    Publication date: July 19, 2012
    Inventor: Jacinthe GINGRAS