Method Of Storing Cells In A Viable State Patents (Class 435/374)
  • Patent number: 10017742
    Abstract: In a process for preparing disinfected preparations from mammalian tissues, mammalian tissue is perfused at one step with an enzyme-free liquid antibiotic composition that includes at least one antibiotic and is contained in at least one perfusion buffer. In a step occurring after such one step, enzymatic antibiotic-free treatment of the tissue is carried out to obtain a single-cell suspension. In another step disinfected preparations are obtained.
    Type: Grant
    Filed: November 12, 2014
    Date of Patent: July 10, 2018
    Assignee: Cytonet GmbH & Co. KG
    Inventors: Krasimira Aleksandrova, Marc Barthold, Lubomir Arseniev, Carsten Griesel, Christoph Priesner
  • Patent number: 9968718
    Abstract: A portable assembly for processing red blood cells RBCs including a disposable blood collection set including a blood bag, an anaerobic storage bag and an oxygen and/or oxygen and carbon dioxide depletion device disposed between the blood collection bag and anaerobic storage bag. The portable assembly further provides for a gas circulation device in fluid communication with the oxygen or oxygen and carbon dioxide depletion device, The gas circulation device includes a pressure source that is able circulate flushing gas through the depletion device as RBCs pass from the blood collection bag, through the depletion device and into the anaerobic storage bag.
    Type: Grant
    Filed: February 4, 2015
    Date of Patent: May 15, 2018
    Assignee: New Health Sciences, Inc.
    Inventors: Tatsuro Yoshida, Paul Vernucci
  • Patent number: 9637513
    Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.
    Type: Grant
    Filed: January 14, 2015
    Date of Patent: May 2, 2017
    Assignee: GenTegra LLC
    Inventors: Michael Hogan, Michael Saghbini, David Wong
  • Patent number: 9585381
    Abstract: Disclosed is an in vitro or ex vivo method for preserving and/or keeping alive mammalian, preferably human skin biopsies, enabling to transport it and, if applicable, to culture it. Also disclosed is a skin biopsy thus preserved and obtained by such a method and relates to its use as a model especially in a kit for screening or selecting cosmetic or therapeutic compounds.
    Type: Grant
    Filed: May 3, 2013
    Date of Patent: March 7, 2017
    Assignee: GENOSKIN
    Inventor: Pascal Descargues
  • Patent number: 9538745
    Abstract: The present invention features novel methods for the cryopreservation of mammalian cell that combine the advantages of the slow-freezing and vitrification approaches while avoiding their shortcomings. Generally, the methods include the use of a capillary tube made of a thermally conductive wall material and a thin wall such that the ratio of the thermal conductivity of the wall material to the wall thickness is at least 1,000-500,000. The solution is then exposed to temperatures equal to or less than ?80° C. and the vitrification solution containing the mammalian cells is cooled at a rate equal to or greater than 30,000-100,000,000° C./minute. The exposure of the capillary tube with a thermally conductive and thin wall allows for vitrification of the solution in the absence of ice formation. Cryoprotectants can also be added to the vitrification solution to further prevent ice formation.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: January 10, 2017
    Assignee: The General Hospital Corporation
    Inventors: Xiaoming He, Thomas L. Toth, Mehmet Toner, Jon Edd
  • Patent number: 9538747
    Abstract: A living cell cryopreservation tool has a living cell holding member having a body part and a living cell holding part and a tubular accommodation member, closed at one end thereof, which is capable of accommodating the living cell holding member. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has heat conductors extended in a longitudinal direction thereof and projected from a distal end thereof. The tubular accommodation member has a heat conductive member accommodated inside a distal portion thereof. When the living cell holding member is inserted into the tubular accommodation member from a distal side of each heat conductor thereof, a distal portion of each heat conductor contacts the heat conductive member of the tubular accommodation member.
    Type: Grant
    Filed: October 1, 2012
    Date of Patent: January 10, 2017
    Inventor: Futoshi Inoue
  • Patent number: 9538746
    Abstract: A living cell cryopreservation tool has a body part formed of a cold-resistant material and a living cell holding part formed of the cold-resistant material. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has a plurality of living cell accommodation concave portions formed in a longitudinal direction thereof and a plurality of excess cryopreservation liquid discharge passages communicating with the living cell accommodation concave portions.
    Type: Grant
    Filed: October 1, 2012
    Date of Patent: January 10, 2017
    Inventor: Futoshi Inoue
  • Publication number: 20150140656
    Abstract: The present invention is a method of freezing cells comprising the steps of incubating said cells in a solution comprising a cryoprotective agent, concentrating the cells resulting from the previous step withdrawing the eluent essentially free of cells, and freezing the resulting concentrated cells. The cells frozen by the invention's method render a high post-thawing viability, reduce cryoprotectant related toxic events and promote cells life in a suspension state after thawing. The invention also comprises the container comprising the frozen cells.
    Type: Application
    Filed: September 16, 2014
    Publication date: May 21, 2015
    Inventors: Roberto HERNAN IZQUIERDO, Natalia Gallot Escobal, Antonio Cruz Pacheco
  • Publication number: 20150140543
    Abstract: Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the disclosed compositions and processes permit mammalian cells, tissues, and organs to be harvested from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity.
    Type: Application
    Filed: December 24, 2014
    Publication date: May 21, 2015
    Inventors: Daniel Andrew Shimko, Scott P. Noel, Susan J. Drapeau, Josee Roy
  • Publication number: 20150125954
    Abstract: The invention relates to a substrate unit (100), in particular for the cryopreservation of a biological sample (1) containing biological cells (2, 2.1, 2.
    Type: Application
    Filed: July 1, 2013
    Publication date: May 7, 2015
    Inventors: Heiko Zimmermann, Axel Beier, Julia Neubauer, Guenter R. Fuhr
  • Publication number: 20150125847
    Abstract: The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.
    Type: Application
    Filed: January 16, 2015
    Publication date: May 7, 2015
    Inventors: Beth H. Shaz, Christopher D. Hillyer
  • Publication number: 20150125540
    Abstract: A composition includes an isolated cell; at least one particle within said cell; and at least one active agent associated with the particle, wherein the active agent is capable of being released from the cell. A method includes administration of such a cell to a subject.
    Type: Application
    Filed: January 5, 2015
    Publication date: May 7, 2015
    Inventors: Jeffrey M. Karp, Debanjan Sarkar, Praveen Kumar Vemula
  • Publication number: 20150118200
    Abstract: Provided is a matrix for promoting survival and differentiation of cells transplanted thereon, comprising a base matrix and a cell-made matrix thereon. Methods and means for making and using same are also provided. Also provided are conditioned media, related compositions, related methods, and related packaging products.
    Type: Application
    Filed: December 24, 2014
    Publication date: April 30, 2015
    Inventors: Ilene SUGINO, Vamsi GULLAPALLI, Marco ZARBIN
  • Publication number: 20150111193
    Abstract: Described herein are compositions and methods particularly useful in the medical arts. The compositions and methods may be used in connection with the preservation of a portion of a mammal, for example, tissues, organs, appendages, limbs, extremities, stem cells, myocytes, bone marrow, skeletal muscle as well as an array of other medical procedures, such as cardiac surgery, transplantation and/or preservation. In various embodiments, the inventive composition may be hyperoxygenated and be formulated to resemble the biochemistries of natural intracellular fluids. The inventive composition includes active ingredients to reduce ischemic, hypothermic and reperfusion injury during transplantation, thereby resulting in improved post-transplant graft function and quality, when used in connection with organ transplantation and storage procedures, for example cardiac transplantation.
    Type: Application
    Filed: October 31, 2014
    Publication date: April 23, 2015
    Inventors: Andreas Kamlot, Alfredo Trento, Lawrence Czer, Jennifer Pass
  • Publication number: 20150110738
    Abstract: The present invention is directed to generating suppressor cells by treating naive T cells with a suppressor-inducing composition such as anti-CD3, anti-CD28, IL-2, TGF-?, or some combination thereof. Such suppressor cells are administered to patients to prevent or treat immune disorders and are allogeneic to the patient.
    Type: Application
    Filed: May 2, 2013
    Publication date: April 23, 2015
    Inventor: David Horwitz
  • Publication number: 20150110723
    Abstract: The disclosure relates to quinazoline derivatives, compositions, and methods related thereto. In certain embodiments, the disclosure relates to inhibitors of NADPH-oxidases (Nox enzymes) and/or myeloperoxidase.
    Type: Application
    Filed: May 28, 2013
    Publication date: April 23, 2015
    Inventors: John David Lambeth, Thota Ganesh, Susan M. Smith, Aiming Sun
  • Patent number: 9012222
    Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: April 21, 2015
    Assignee: Auxocell Laboratories, Inc.
    Inventor: Rouzbeh R. Taghizadeh
  • Publication number: 20150093361
    Abstract: This invention provides disc stem cells, processes for obtaining and culturing disc stein cells, and methods for repairing damaged or diseased disc tissue comprising the use of the disc stem cells of the invention.
    Type: Application
    Filed: February 14, 2012
    Publication date: April 2, 2015
    Applicant: DISCGENICS
    Inventors: Valery Kukekov, Umar Akbar, Christopher Duntsch
  • Publication number: 20150087056
    Abstract: The present invention provides a method for cryoprotecting a biological specimen comprising the step of freezing said biological specimen in the presence of a hydrogel and in the absence of cryoprotectant.
    Type: Application
    Filed: January 17, 2013
    Publication date: March 26, 2015
    Inventors: Anne Pelle Meddahi, Aicha Abed, Didier Letourneur, Anne Baudot
  • Publication number: 20150079190
    Abstract: The invention is directed to immortalized cell compositions and compositions derived therefrom. The invention is further directed to methods of making and using such immortalized cell compositions and compositions derived therefrom. Such immortalized cell compositions include but are not limited to Immortalized Amnion-derived Multipotent Progenitor cells (herein referred to as I-AMP cells) and conditioned media derived therefrom (herein referred to as I-Amnion-derived Cellular Cytokine Solution or I-ACCS).
    Type: Application
    Filed: November 20, 2014
    Publication date: March 19, 2015
    Applicant: STEMNION, INC.
    Inventors: Randall G. Rupp, Linda O. Palladino
  • Publication number: 20150072424
    Abstract: One aspect of the present invention is directed to an apparatus for cryogenically cooling a substance. The apparatus of the present invention includes a thin film evaporator comprising a microstructured surface and an applicator for dispensing a working fluid onto the microstructured surface. The apparatus preferably contains a pressure-controlled vessel enclosing the thin film evaporator and at least a portion of the applicator. In a second aspect, the present invention is directed to the method of using a thin film evaporator of the present invention to cryogenically cool a substance by forming a thin film layer of the working fluid on the microstructured surface of the thin film evaporator.
    Type: Application
    Filed: September 10, 2014
    Publication date: March 12, 2015
    Inventors: HONGBIN MA, Fengmin Su, Xu Han, Hsiu-Hung Chen
  • Publication number: 20150072340
    Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
    Type: Application
    Filed: September 28, 2012
    Publication date: March 12, 2015
    Applicant: INGURAN, LLC
    Inventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
  • Publication number: 20150072423
    Abstract: A method of thawing cryopreserved human hepatocytes and cryopreserving the hepatocytes a second time without losing viability is described. It allows the preparation of cryopreserved human hepatocytes pooled from multiple donors by thawing the hepatocytes from the individual donors, combining the cells to form pooled hepatocytes, and recryopreserving the pooled hepatocytes. The method involves the thawing of the hepatocytes from the individual donors, maintaining the thawed hepatocytes at a low temperature that is above freezing temperature (e.g., 4° C.), and refreezing the thawed cells without further manipulation. The method allows the cryopreserving the human hepatocytes a second time after thawing, with viability similar to that after one single cryopreservation. This high efficiency method can be used for the preparation of highly viable pooled human hepatocytes for experimentation to minimize individual variations.
    Type: Application
    Filed: September 10, 2013
    Publication date: March 12, 2015
    Inventor: Albert Li
  • Publication number: 20150064133
    Abstract: The present disclosure provides a composition comprising a bioactive fraction derived from a platelet concentrate, methods of making the bioactive fraction, and culture medium supplemented with the bioactive fraction. Preferred bioactive fractions have relatively low fibrinogen concentrations while retaining native growth factors in beneficial amounts and ratios.
    Type: Application
    Filed: August 27, 2014
    Publication date: March 5, 2015
    Inventors: Erik J. Woods, Christopher Taylor
  • Patent number: 8968991
    Abstract: Numerous embodiments of a system and method for treating cardiac tissue are described. In one embodiment, bone marrow cells are extracted from a patient. The cells are then processed to isolate mononuclear cells, which can then be delivered back near the cardiac tissue of the patient.
    Type: Grant
    Filed: February 15, 2008
    Date of Patent: March 3, 2015
    Assignee: Abbott Cardiovascular Systems Inc.
    Inventors: Evgenia Mandrusov, Albert K. Chin, William Earl Webler, Jr., Yan Shen, Robert D. Alnsworth, Eugene Michal
  • Patent number: 8961962
    Abstract: The present invention relates to methods of promoting the survival of cells by treating the cells with acid ceramidase. A kit for promoting ex vivo cell survival is also disclosed, as is a method of predicting in vitro fertilization outcome of a female subject.
    Type: Grant
    Filed: January 7, 2008
    Date of Patent: February 24, 2015
    Assignee: Mount Sinai School of Medicine
    Inventors: Edward H. Schuchman, Efrat Eliyahu, Nataly Shtraizent, Xingxuan He
  • Patent number: 8961429
    Abstract: An arrangement for collecting a tissue or stool sample has a sample vial and a source of preservative fluid. A fluid valve controls a flow of the preservative fluid from the source of preservative fluid to the sample vial. An air equalization vent tube is coupled to the source of preservative fluid and an air valve controls a flow of air through the air equalization vent tube, the air valve and the fluid valve being actuated simultaneously. A scoop for collecting the sample is coupled to the sample vial coupler and extends into the sample vial. The preservative fluid flows from the source of preservative fluid to the sample vial by force of gravity upon actuation of the fluid valve. The air equalization vent tube transfers air from the sample vial to the source of preservative fluid upon actuation of the air valve.
    Type: Grant
    Filed: September 19, 2008
    Date of Patent: February 24, 2015
    Assignee: Wayne State University
    Inventors: Jeffrey L. Ram, Jordan Nechvatal, Ikuko Kato
  • Publication number: 20150044176
    Abstract: The invention provides improved methods for preparing hematopoietic cells for transplantation and the resulting improved hematopoietic cell compositions. The invention further relates to improved culture media and methods of culturing, processing, modulating, and expanding blood cell products for hematopoietic transplantation.
    Type: Application
    Filed: March 13, 2014
    Publication date: February 12, 2015
    Inventor: Betsy Denise REZNER
  • Patent number: 8951719
    Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.
    Type: Grant
    Filed: August 20, 2012
    Date of Patent: February 10, 2015
    Assignee: Gentegra, LLC.
    Inventors: Michael Hogan, Michael Saghbini, David Wong, Chunnian Shi
  • Publication number: 20150037316
    Abstract: The present invention provides placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. The invention also provides methods of differentiating the placental stem cells. The invention further provides methods of using the placental stem cells in assays and for transplanting.
    Type: Application
    Filed: June 6, 2014
    Publication date: February 5, 2015
    Inventors: James Edinger, Qian Ye, Jai-Lun Wang, Robert J. Hariri
  • Publication number: 20150037783
    Abstract: An improved cryopreservation process and substances can involve a cellular collection (1) in a cryopreservation fluid (4) that has been conditioned or treated (7) to enhance the cryopreservation process by adding (18) energy (19) such as in the surface energy of a substance in the cryopreservation fluid (4) prior to reducing energy for that same cryopreservation media for freezing. This can offer enhanced-post-cryogenic viability of the cryopreserved structures or a more optimum cooling curve (22) for a specific cell type.
    Type: Application
    Filed: March 11, 2013
    Publication date: February 5, 2015
    Inventors: Lisa A. Herickhoff, Patrick D. Burns, Nicole R. White, James A. Herickhoff
  • Publication number: 20150037782
    Abstract: Novel methods and systems for electromagnetic freezing are disclosed. An oscillating magnetic field can be applied in conjunction with a static magnetic field in order to align magnetic particles inside biological tissues. Such chains may be naturally present or artificially introduced. Needle-shaped electrodes may be used to produce ions and disturb the air layers insulating the tissues to be frozen.
    Type: Application
    Filed: July 10, 2014
    Publication date: February 5, 2015
    Inventors: Atsuko KOBAYASHI, Joseph L. KIRSCHVINK
  • Patent number: 8945486
    Abstract: A microwell device is provided. The device includes a plate having a upper surface. The upper surface has first and second recesses formed therein. Each recess has an outer periphery. First and second portions of microwells are formed in upper surface of the plate. The first portion of microwells are spaced about the outer periphery of the first recess and the second portion of microwells spaced about the outer periphery of the first recess. A first barrier is about a first portions of the microwells for fluidicly isolating the first portion of the microwells and a second barrier about a second portions of microwells for fluidicly isolating the second portion of the microwells.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: February 3, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jay W. Warrick, John Yin, Stephen M. Lindsay
  • Publication number: 20150030571
    Abstract: Preparation and method for producing a preparation or a tissue derivative that include mesenchymal stem cells, to be used in cellular therapy, for cosmetic treatments, for replacing a tissue or an organ, or inducing or accelerating tissue repair or regeneration. The method includes the steps of extracting tissue containing mesenchymal stem cells, such as adipose tissue, from a cadaveric donor by liposuction or by surgical removal of parts of adipose tissue, and mechanically treating tissue to provide a fluid component, having an oily component, a blood component and/or a sterile solution, and a solid component having cell fragments, cells and one or more cell macro-agglomerates of heterogeneous sizes, thereby separating and removing the fluid component from the solid component, which generates an emulsion of fluid components.
    Type: Application
    Filed: March 27, 2013
    Publication date: January 29, 2015
    Inventor: Carlo Tremolada
  • Publication number: 20150024406
    Abstract: A method of storing mammalian cells or tissue (e.g., liver cells or hepatocytes) for subsequent use comprises the steps of: (a) contacting the cells or tissue in vitro to a choleretic agent in an effective amount; (b) combining said cells or tissue with a cryopreservative; (c) freezing said cells or tissue, and then (d) storing said frozen cells or tissue in frozen form for subsequent use.
    Type: Application
    Filed: July 14, 2014
    Publication date: January 22, 2015
    Inventors: Robin N. Coger-Simmons, Laura Whritenour Schrum, Balasubramanian Karthik Kumar, Charles Lee
  • Publication number: 20150024487
    Abstract: The present invention relates to a cryopreservative device comprising an outer case, one or more layers of space for the cryo-bags, and two or more Teflon cryopreservative bags. The outer case has a cover lip for opening and closing. The Teflon cryopreservative bags are filled with a freezing resistant. In the present invention, the cryo-bags and the Teflon cryopreservative bags are crossly stacked in the cryopreservation device. The Teflon cryopreservative bags are designed to directly contact with the cryo-bags in order to obtain the effect of slow cell freezing.
    Type: Application
    Filed: January 8, 2014
    Publication date: January 22, 2015
    Applicant: Steminent Biotherapeutics, Inc.
    Inventors: Cheng-Yi CHENG, Yu-Shan WU, Yin LIANG, Wei-Kee ONG, Hui-Chun HO
  • Publication number: 20150017129
    Abstract: The instant invention is the storage of Type II collagen containing tissue in carbonated water. Such Type II collagen is useful for alleviating the symptoms of arthritis in mammals as well as the treatment of arthritis in mammals. Such Type II collagen is also useful for the prevention of arthritis in mammals. The instant invention is also a method for the preparation of a nutritional supplement that includes the steps of: (a) separating water-insoluble undenatured Type II collagen containing animal tissue from animal tissue not containing Type II collagen; (b) subdividing and sterilizing said tissue under conditions which do not change the original structure of the Type II collagen to produce a subdivided and sterilized product; (c) packaging the subdivided and sterilized product in carbonated water.
    Type: Application
    Filed: March 14, 2014
    Publication date: January 15, 2015
    Inventor: Eugene R. Moore
  • Publication number: 20150017628
    Abstract: The present invention relates to a method for cryopreserving biological material. In particular, the method comprises storing the biological material at a cryopreserving temperature in a composition comprising polyvinyl alcohol (PVA). wherein the composition is substantially free of vitrification-inducing agents such as DMSO and glycerol. The invention also provides methods of inhibiting ice recrystallisation and of reducing cell damage during the warming or thawing of a cryopreserved composition comprising biological material. The invention also relates to processes for producing a biological material, and related kits.
    Type: Application
    Filed: February 7, 2013
    Publication date: January 15, 2015
    Inventors: Matthew I. Gibson, Robert C. Deller
  • Publication number: 20150010506
    Abstract: This invention provides a therapeutic placental composition comprising placental cells and other placental components derived from placental tissue. A cryopreserved placental composition is also provided. The placental compositions can be used to stimulate and promote angiogenesis, reduce inflammation, and to reduce scar formation, among others. The placental tissue can optionally be an amnion, chorion, a trophoblast-depleted chorion, umbilical cord, Wharton's jelly, placental cotyledon, and/or maternal decidua. The placental composition of the present invention is useful in treating a patient with a tissue injury (e.g., wound or burn) by applying the placental composition to the injury or in close proximity. Placental compositions may also be used to promote or increase regeneration of tissue. Similar application is useful with ligament and tendon repair and for engraftment procedures such as bone engraftment.
    Type: Application
    Filed: May 7, 2014
    Publication date: January 8, 2015
    Inventors: Timothy Jansen, Samson Tom, Alla Danilkovitch, Dana Yoo, Jaime Zerhusen, Gabriele Putz Todd, Amy Elizabeth Johnson
  • Publication number: 20150011000
    Abstract: The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.
    Type: Application
    Filed: September 22, 2014
    Publication date: January 8, 2015
    Inventor: Tomas Stojanov
  • Publication number: 20150011429
    Abstract: Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable freezing quality of the adipose-derived stem cells and influence of harmful factors in serum on the adipose-derived stem cells are solved, and the adipose-derived stem cells stored have the advantages of high survival percentage, well adherence growth and strong differentiation capacity.
    Type: Application
    Filed: August 6, 2012
    Publication date: January 8, 2015
    Inventors: Helen Zhang, Luyi Zhang, Wei Cao
  • Publication number: 20150004694
    Abstract: Synthetic storage media are disclosed for use in the processing and the storing of platelets. The storage media includes a platelet storage solution and less than 20 percent plasma to preserve platelet function after at least 7 days of storage.
    Type: Application
    Filed: August 18, 2014
    Publication date: January 1, 2015
    Inventors: Veronique Mayaudon, Jean-Marc Payrat
  • Publication number: 20150004693
    Abstract: Mesenchymal stem cells which express TNF-? receptor Type I in an amount of at least 13 pg/106 cells. Such mesenchymal stem cells inhibit the proliferation of lymphocytes and may be employed, in particular, in the treatment of graft-versus-host disease.
    Type: Application
    Filed: September 12, 2014
    Publication date: January 1, 2015
    Inventors: Alla Danilkovitch, Diane Carter, Alicia Tyrell, Simon Bubnic, Michelle Marcelino, Rodney Monroy
  • Publication number: 20140377234
    Abstract: The present disclosure relates to a method of expanding myeloid progenitor cells by culturing an initial population of cells in a medium comprising a mixture of cytokines and growth factors that promote growth and expansion of the myeloid progenitor cells. The expanded cell population provides a source of cells as therapeutic treatments for neutropenia and/or thrombocytopenia arising in patients subjected to myeloablative therapy and hematopoietic stem cell transplantation.
    Type: Application
    Filed: September 9, 2014
    Publication date: December 25, 2014
    Inventors: Timothy C. Fong, Adrianus Geertrudis Wilhelmus Domen, Julie Lynne Christiansen
  • Publication number: 20140378380
    Abstract: The present invention relates to methods for protecting a subject, tissues and/or organs from a subject against oxidative stress-induced damage, such as but not limited to, oxidative stress-induced tissue damage. The method comprises administering an effective amount of unacylated ghrelin, a fragment thereof, an analog thereof and/or pharmaceutically acceptable salts thereof to the subject.
    Type: Application
    Filed: June 20, 2014
    Publication date: December 25, 2014
    Inventors: Maria Felice Brizzi, Ezio Ghigo
  • Publication number: 20140356948
    Abstract: There is provided a method for stably preserving cells and valuable materials included in the cells at room temperature by lyophilizing the cells. When the cells are lyophilized using a lyoprotectant, damage to the structure of the cells which may otherwise occur during a freezing process can be prevented, the lyophilized cells can be stored at room temperature, and the valuable material included in the cells may maintain the functions thereof at room temperature.
    Type: Application
    Filed: December 10, 2012
    Publication date: December 4, 2014
    Applicant: TEGO SCIENCE INC.
    Inventors: Saewha Jeon, Ki Hoon Lee, Hankyu Jang
  • Publication number: 20140356953
    Abstract: The invention relates to a dispensing unit for dispensing fluid into an external container. The dispensing unit comprises: a connector, a reagent cylinder for storing the fluid, and a locking ring. The connector comprises an upper fastener, and a bottom wall with at least one passage therethrough. The reagent cylinder comprises at least one compartment for storing the fluid, which compartment comprises at least one opening through a cylinder bottom. The at least one opening is located so that for at least one mutual orientation of the reagent cylinder and the connector, fluid can flow from the compartment to the external container via the at least one opening and the at least one passage. The locking ring comprises a second fastener which fastens to the upper fastener of the connector, so that when the locking ring is tightened, the reagent cylinder is pressed towards the interior bottom of the connector.
    Type: Application
    Filed: December 14, 2012
    Publication date: December 4, 2014
    Inventors: Asger Hangstrup Høgsaa, Bjarke Hangstrup Høgsaa, Sebastian André Jensen Langhoff
  • Patent number: 8900864
    Abstract: A method of producing extremely stable leukocytes from human blood samples which are stable for long period of time under extraordinary temperature conditions. The method also relates to producing and stabilizing the leukocytes with formaldehyde release agents. The method is directed at the use of the stabilized leukocytes as a control in assays for determining CD4+ and HIV diagnosis and therapy.
    Type: Grant
    Filed: September 13, 2011
    Date of Patent: December 2, 2014
    Assignee: Nordrhein-Wesfalen Krankenhausbetriebsgesellschaft Bad Oeynhausen mbH
    Inventors: Christian Prante, Knut Kleesiek, Wolfgang Prohaska
  • Patent number: 8900863
    Abstract: Provided herein are methods and kits for the isolation, processing and cryopreservation of mesenchymal cells from the Wharton's Jelly and vascular progenitor cells from umbilical cord tissue. Also provided are isolated mesenchymal cells or vascular progenitor cells obtained by the invention methods, and compositions thereof.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: December 2, 2014
    Assignee: Lifeline Cord Blood Bank
    Inventors: Zacharias Kallis, Kyriacos Matsis
  • Patent number: 8901099
    Abstract: It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: December 2, 2014
    Assignee: Sekisui Chemical Co., Ltd.
    Inventors: Joe Chiba, Jun-ichi Hata, Naoki Nishiguchi, Masahiro Furutani