Method Of Altering The Differentiation State Of The Cell Patents (Class 435/377)
  • METHODS FOR TRANSDIFFERENTIATION OF BODY TISSUES
    Publication number: 20140094425
    Abstract: The invention relates to methods for transdifferentiation of body tissues which can be used to generate specific cell types needed for regenerating organs or body parts, following cellular degeneration, injury or amputation. The present invention also describes the use of tissue transdifferentiation for treating cancer and autoimmune diseases.
    Type: Application
    Filed: September 25, 2013
    Publication date: April 3, 2014
    Inventor: Steven BARANOWITZ
  • MAMMALIAN CYTOKINES; RECEPTORS; RELATED REAGENTS AND METHODS
    Publication number: 20140093875
    Abstract: Nucleic acids encoding mammalian cytokine receptor, e.g., for cytokine IL-B50, purified proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are described.
    Type: Application
    Filed: December 2, 2013
    Publication date: April 3, 2014
    Applicant: Merck Sharp & Dohme Corp.
    Inventors: Pedro A. Reche-Gallardo, Vassili Soumelis, Yong-Jun Liu, Rene de Waal Malefyt, J. Fernando Bazan, Robert A. Kastelein
  • METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELLS AND ITS APPLICATIONS
    Publication number: 20140093486
    Abstract: The present invention relates to a novel method for preparing induced pluripotent stem cells (iPSCs) by introducing three genes, Oct3/4, Sox2, and Parp1, into somatic cells. The present invention also relates to the iPSCs produced by the aforementioned method. Also provided is a method of rejuvenating cells by use of a PARylated protein or an enzyme with PARylation activity. Further provided is a method for inducing the secretion of interferon-? inducible protein-10 (IP-10) comprising administering to a subject in need thereof an effective amount of iPSCs or iPSC-CM.
    Type: Application
    Filed: October 1, 2013
    Publication date: April 3, 2014
    Applicant: Taipei Veterans General Hospital
    Inventors: SHIH-HWA CHIOU, YUEH CHIEN, GUANG-YUH CHIOU, YI-PING YANG
  • CELL DIFFERENTIATION INDUCER AND DIFFERENTIATION INDUCING METHOD
    Publication number: 20140093960
    Abstract: The objective of the present invention is to provide a cell differentiation inducer which is a low-molecular compound, which can be chemically synthesized easily and which can efficiently induce a differentiation of an undifferentiated cell into a nervous system cell with high selectivity. In addition, the objective of the present invention is to provide use of a specific catechol derivative for efficiently inducing a differentiation of an undifferentiated cell into a nervous system cell with high selectivity, and a method for efficiently inducing a differentiation of an undifferentiated cell into a nervous system cell with high selectivity using a specific catechol derivative. The cell differentiation inducer of the present invention is characterized in comprising the catechol derivative having the specific chemical structure.
    Type: Application
    Filed: May 14, 2012
    Publication date: April 3, 2014
    Applicant: The University of Tokushima
    Inventors: Daisuke Tsuji, Kohji Itoh, Shigeki Sano, Michiyasu Nakao
  • Cellular Substrate for Nuclear Reprogramming
    Publication number: 20140093963
    Abstract: This invention relates to the use of late out-growth endothelial progenitor cells (L-EPCs) as a cellular substrate for the generation of Induced pluripotent stem cells (iPSCs). This may be useful in the production of patient-specific tissues for disease modelling, drug and toxicology screening, tissue replacement and delivery of gene therapy.
    Type: Application
    Filed: March 30, 2012
    Publication date: April 3, 2014
    Applicant: Cambridge Enterprise Limited
    Inventors: Nicholas Morrell, Mark Ormiston, Amer Rana, Ludovic Vallier
  • Small molecules supporting pluripotent cell growth and methods thereof
    Patent number: 8685726
    Abstract: The present invention relates to compositions and methods for maintaining undifferentiated pluripotent stem cell cultures.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: April 1, 2014
    Assignee: Viacyte, Inc.
    Inventors: Thomas C Schulz, Allan J Robins
  • Nucleic acid aptamers
    Patent number: 8685937
    Abstract: The present invention relates to optimized aptamers and methods of using these aptamers.
    Type: Grant
    Filed: August 6, 2009
    Date of Patent: April 1, 2014
    Assignee: University of Iowa Research Foundation
    Inventors: Paloma H. Giangrande, James O. McNamara, Anton P. McCaffrey
  • Impaired wound healing compositions and treatments
    Patent number: 8685940
    Abstract: Connexin modulation for the treatment of wounds that do not heal at expected rates, including delayed healing wounds, incompletely healing wounds, and chronic wounds, and associated methods, compositions and articles.
    Type: Grant
    Filed: November 11, 2011
    Date of Patent: April 1, 2014
    Assignee: CoDa Therapeutics, Inc.
    Inventors: David Laurence Becker, Colin Richard Green, Bradford J. Duft
  • Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
    Patent number: 8685730
    Abstract: Methods and devices for culturing human pluripotent stem cells to produce cells of the pancreatic lineage are disclosed. The methods include steps of culturing the stem cells under conditions that induce the expression of mesendoderm/primitive streak and definitive endoderm markers in a chemically defined medium including an effective amount of i) fibroblast growth factor, ii) Activin A, and iii) bone morphogenetic protein. The methods further include the steps of culturing cells under conditions favoring the formation of at least one of intact embryoid bodies and pancreatic progenitor PDX1+Ins? cells.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: April 1, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jon Odorico, Xiaofang Xu
  • METHOD OF DERIVING STEM CELLS, STEM CELLS, AND USE OF STEM CELLS FOR WOUND HEALING
    Publication number: 20140088563
    Abstract: A method of deriving isolated stem cells including: implanting a matrix in a wound site of a living organism; allowing cells to infiltrate the matrix; removing the matrix containing the infiltrated cells from the wound site; and removing the infiltrated cells from the matrix to provide isolated stem cells. Stem cells produced by this process, stem cells with certain characteristics, and methods for treating wounds using these stem cells are provided.
    Type: Application
    Filed: September 27, 2012
    Publication date: March 27, 2014
    Applicant: EMBRO CORPORATION
    Inventors: Vance D. Fiegel, David R. Knighton
  • INDUCTION OF HUMAN EMBRYONIC STEM CELL DERIVED CARDIAC PACEMAKER OR CHAMBER-TYPE CARDIOMYOCYTES BY MANIPULATION OF NEUREGULIN SIGNALING
    Publication number: 20140087460
    Abstract: The present invention is directed to methods of producing cardiomyocytes having a nodal/pacemaker phenotype and cardiomyocytes having an atrial/ventricular phenotype. Isolated populations of nodal/pacemaker and atrial/ventricular cardiomyocytes are also disclosed. Methods of treating a subject having cardiac arrhythmia and a subject in need of cardiac tissue repair using the isolated populations of nodal/pacemaker cardiomyocytes and atrial/ventricular cardiomyocytes, receptively, are also disclosed.
    Type: Application
    Filed: October 23, 2013
    Publication date: March 27, 2014
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Michael A. Laflamme, Wei-Zhong Zhu
  • MEANS AND METHODS FOR ACTIVE CELLULAR IMMUNOTHERAPY OF CANCER BY USING TUMOR CELLS KILLED BY HIGH HYDROSTATIC PRESSURE AND DENDRITIC CELLS
    Publication number: 20140086957
    Abstract: Disclosed are pharmaceutical compositions for inducing an immune response against tumor cells comprising tumor cells which are made apoptotic by treatment with high hydrostatic pressure and dendritic cells, and methods for producing such compositions.
    Type: Application
    Filed: July 4, 2012
    Publication date: March 27, 2014
    Applicant: SOTIO a.s.
    Inventors: Jirina Bartunková, Radek Spisek
  • METHODS AND COMPOSITIONS FOR PRODUCING INDUCED HEPATOCYTES
    Publication number: 20140087416
    Abstract: The present invention relates to methods and compositions for use in generating induced hepatocytes by reprogramming non-hepatocyte cells.
    Type: Application
    Filed: September 6, 2013
    Publication date: March 27, 2014
    Applicant: Genentech, Inc.
    Inventors: Kamen P. Simeonov, Hirdesh Uppal
  • COMPOUNDS AND METHODS FOR ALTERING ACTIVIN RECEPTOR-LIKE KINASE SIGNALING
    Publication number: 20140088174
    Abstract: Described are compounds and methods useful in the promotion of muscle growth, the treatment of muscle loss or insufficient muscle growth, and the treatment of fibrotic conditions.
    Type: Application
    Filed: April 5, 2012
    Publication date: March 27, 2014
    Applicant: Academisch Ziekenhuis Leiden h.o.d.n. LUMC
    Inventors: Peter Abraham Christiaan "T Hoen, Wilhelmus Martinus Hendrikus Hoogaars, Dwi Utami Kemaladewi, Adriana Marie Rus, Peter Ten Duke
  • MULTIPOTENT NEURAL CELLS
    Publication number: 20140087464
    Abstract: The inventions disclosed herein are based on the identification of novel cell populations derived from human embryonic stem cells and other pluripotent cells. The inventive cell populations may be used for cell therapies for the treatment of various neurological diseases and as substrates in pharmacological assays.
    Type: Application
    Filed: September 26, 2013
    Publication date: March 27, 2014
    Applicant: THE MCLEAN HOSPITAL CORPORATION
    Inventors: Ole Isacson, Jan Pruszak
  • STEM CELL PREPARATIONS AND METHODS OF USE
    Publication number: 20140086882
    Abstract: The present invention relates to a method of preparing cells and in particular to a method of preparing breastmilk stem cells (BSCs) by isolation from breastmilk and subsequent culture. The invention further relates to BSCs prepared by the methods of the invention and to methods and uses thereof. The invention has been developed primarily as a method for preparing and culturing BSC.
    Type: Application
    Filed: April 19, 2013
    Publication date: March 27, 2014
    Applicant: Medela Holding AG
    Inventor: Foteini Hassiotou
  • Method for increasing the pool of Ngn3+ endocrine progenitor cells and pancreatic endocrine cell mass
    Patent number: 8679842
    Abstract: The present invention discloses a method for increasing the pool of Ngn3+ endocrine progenitor cells obtained from stem cells, by contacting said stem cells with a SUR1/Kir6.2 channel inhibitor. The invention also relates to a method for increasing the mass of pancreatic endocrine cells, in particular of ? cells. The invention further concerns a method for treating diabetes.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: March 25, 2014
    Assignee: Assistance Publique—Hopitaux de Paris
    Inventors: Michel Polak, Raphaël Scharfmann, Samia Zertal-Zidani
  • Compositions for the preparation of mature dendritic cells
    Patent number: 8679840
    Abstract: The invention relates to a method for in vitro maturation of at least one immature dendritic cell, comprising stimulating said immature dendritic cell with TNF?, IL- 1?, IFN?, a TLR7/8 agonist and prostaglandin E2 (PG). Furthermore, the invention relates to a composition comprising these factors as well as to mature dendritic cells produced by a method of the invention.
    Type: Grant
    Filed: March 28, 2007
    Date of Patent: March 25, 2014
    Assignee: Helmholtz Zentrum München Deutsches Forschungszentrym für Gesundheit und Umwelt (Gmbh)
    Inventors: Dolores J. Schendel, Anke Zobywalski, Iris Bigalke
  • Tumor-initiating cells and methods of use
    Patent number: 8679835
    Abstract: Provided herein are an isolated or enriched population of tumor initiating cells derived from normal cells, cells susceptible to neoplasia, or neoplastic cells. Methods of use of the cells for screening for anti-hyperproliferative agents, and use of the cells for animal models of hyperproliferative disorders including metastatic cancer, diagnostic methods, and therapeutic methods are provided.
    Type: Grant
    Filed: June 30, 2009
    Date of Patent: March 25, 2014
    Assignee: GeneSys Research Institute
    Inventor: Lynn Hlatky
  • DERIVATION OF FIBROCHONDROCYTES FROM PROGENITOR CELLS
    Publication number: 20140079739
    Abstract: Provided herein are compositions and methods for forming fibrochondrocytes or fibrochondrocyte-like cells from progenitor cells, such as mesenchymal stem cells. One aspect provides a fibrochondrocyte culture medium including CTGF and TGF?3, optionally encapsulated by microspheres having different release profiles. Another aspect provides a method for forming fibrochondrocytes or fibrochondrocyte-like cells from progenitor cells by culturing with CTGF and TGF?3.
    Type: Application
    Filed: October 3, 2011
    Publication date: March 20, 2014
    Applicant: The Trustees of Columbia University in the City of New York
    Inventors: Jeremy J. Mao, Chang Hun Lee
  • Compositions and Methods of Using Differentiated Cells Sensitized to Botulinum Neurotoxin
    Publication number: 20140080142
    Abstract: Differentiated cholinergic cells having motor neuron-like morphology and increased sensitivity to botulinum neurotoxin are provided herein. Methods of using such differentiated cells for detecting neurotoxin are also provided.
    Type: Application
    Filed: February 28, 2013
    Publication date: March 20, 2014
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Eric Arthur Johnson, Regina Whitemarsh, Sabine Pellett
  • Generation of NK Cells and NK-Cell Progenitors
    Publication number: 20140080148
    Abstract: The present invention provides a cytokine-based culture method for ex vivo expansion of NK cells from postembryonic hematopoietic stem cells into a fully closed, large-scale, cell culture bioprocess. We optimized enrichment of CD34+ cells followed by efficient expansion in gas-permeable cell culture bags. Thereafter, expanded CD34+ cells could be reproducibly amplified and differentiated into CD56+CD3? NK cell products with a mean expansion of more than 2,000 fold and a purity of >90%. Also provided are collections of cultured cells having specific properties.
    Type: Application
    Filed: March 16, 2012
    Publication date: March 20, 2014
    Inventor: Jan Spanholtz
  • Treatment of Pluripotent Cells
    Publication number: 20140080210
    Abstract: The present invention is directed to methods to treat pluripotent cells, whereby the pluripotent cells can be efficiently expanded in culture and differentiated by treating the pluripotent cells with an inhibitor of GSK-3B enzyme activity.
    Type: Application
    Filed: November 20, 2013
    Publication date: March 20, 2014
    Applicant: JANSSEN BIOTECH, INC.
    Inventors: Janet Davis, Jiajian Liu
  • Compounds and methods for modulating expression ApoB
    Patent number: 8673871
    Abstract: The present disclosure describes short antisense compounds, including such compounds comprising chemically-modified high-affinity monomers 8-16 monomers in length. Certain such short antisense compound are useful for the reduction of target nucleic acids and/or proteins in cells, tissues, and animals with increased potency and improved therapeutic index. Thus, provided herein are short antisense compounds comprising high-affinity nucleotide modifications useful for reducing a target RNA in vivo. Such short antisense compounds are effective at lower doses than previously described antisense compounds, allowing for a reduction in toxicity and cost of treatment. In addition, the described short antisense compounds have greater potential for oral dosing.
    Type: Grant
    Filed: May 7, 2007
    Date of Patent: March 18, 2014
    Assignee: Isis Pharmaceuticals, Inc.
    Inventors: Sanjay Bhanot, Richard S. Geary, Robert McKay, Brett P. Monia, Punit P. Seth, Andrew M. Siwkowski, Eric E. Swayze, Edward Wancewicz
  • Cell culture support and cell culture method
    Patent number: 8673638
    Abstract: The present invention relates to a cell culture support for culturing mesenchymal stem cells, which includes en upper surface including a plurality of wells, in which the upper surface has a root mean square roughness Rq of 100 to 280 nm and a linear density of 1.6 to 10 per 1 ?m length.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: March 18, 2014
    Assignee: Covalent Materials Corporation
    Inventors: Fumihiko Kitagawa, Takafumi Imaizumi, Shunsuke Takei, Itsuki Yamamoto, Yasuhiko Tabata
  • Human multipotent germ line stem cells expressing a germ line marker and a pluripotency marker produced by co-culture of testicular tissue
    Patent number: 8673637
    Abstract: A method of in vitro maturation of adult human germ line cells in an artificial biological environment, which entails: a) isolating human spermatogonial stem cells (SSCs), and optionally purifying the same; and b) co-culturing the isolated and optionally purified SSCs with a suitably adjusted Sertoli cell environment to obtain haploid germ cells.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: March 18, 2014
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Renee Reijo Pera, Paul J. Turek, Juanito Meneses, Nina Kossack
  • GENERATION OF NATURAL KILLER CELLS AND LYMPHOID TISSUE INDUCER-LIKE (LTI-LIKE) NK-22 CELLS
    Publication number: 20140072545
    Abstract: The present invention relates generally to methods to prepare NK and LTi-like, NK22 cells from HSCs and uses of those cells.
    Type: Application
    Filed: December 2, 2011
    Publication date: March 13, 2014
    Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Michael Verneris, Jeff Miller, Bruce Blazar, Yong-Oon Ahn
  • METHOD FOR GENERATING IMMUNOMODULATORY CELLS, THE CELLS PREPARED THEREFROM AND USE THEREOF
    Publication number: 20140073569
    Abstract: The present invention develops a straightforward and rapid method for generating immunomodulatory cells from peripheral mononuclear cells, comprising treating peripheral mononuclear cells with a hepatocyte growth factor (HGF) to induce differentiation of the peripheral mononuclear cells into immunomodulatory leukocytes. The present invention also provides an immunomodulatory cell prepared according to this method. The present invention further provides a method for treating a disease caused by abnormal immune response comprising administering a HGF to a patient exhibiting the disease, inducing the patient's peripheral mononuclear cells to differentiate into immunomodulatory leukocytes, and modulating the abnormal immune response.
    Type: Application
    Filed: September 10, 2012
    Publication date: March 13, 2014
    Applicant: National Health Research Institutes
    Inventors: Lin-Ju Yen, Ko-Jiunn Liu, Huey-Kang Sytwu
  • MESENCHYMAL STROMAL CELLS AND USES RELATED THERETO
    Publication number: 20140072537
    Abstract: The present invention generally relates to novel preparations of mesenchymal stromal cells (MSCs) derived from hemangioblasts, methods for obtaining such MSCs, and methods of treating a pathology using such MSCs. The methods of the present invention produce substantial numbers of MSCs having a potency-retaining youthful phenotype, which are useful in the treatment of pathologies.
    Type: Application
    Filed: May 30, 2013
    Publication date: March 13, 2014
    Inventors: Erin Anne KIMBREL, Robert LANZA, Jianlin CHU, Nicholas Arthur KOURIS
  • METHODS FOR TRANSFECTING CELLS WITH NUCLEIC ACIDS
    Publication number: 20140073053
    Abstract: The present disclosure provides culture media and methods of using culture media for efficient transfection of a target cell with nucleic acid molecules. The media is capable of supporting cells in culture that are differentiating, transdifferentiating, and/or dedifferentiating.
    Type: Application
    Filed: September 6, 2013
    Publication date: March 13, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Mehmet Fatih Yanik, Matthew Angel
  • EX VIVO, FAST AND EFFICIENT PROCESS TO OBTAIN ACTIVATED ANTIGEN-PRESENTING CELLS THAT ARE USEFUL FOR THERAPIES AGAINST CANCER AND IMMUNE SYSTEM-RELATED DISEASES
    Publication number: 20140072596
    Abstract: The present invention relates to an ex vivo, fast and efficient process to obtain activated antigen-presenting cells that are useful for therapies against cancer and immune system-related diseases. At the same time, it is related to a cellular composition that contributes to stimulate the activated antigen-presenting cells to induce a specific immune response against tumors in patients with cancer or other pathologies involving immune responses.
    Type: Application
    Filed: May 1, 2013
    Publication date: March 13, 2014
    Applicants: ONCOBIOMED, UNIVERSIDAD DE CHILE
    Inventors: UNIVERSIDAD DE CHILE, ONCOBIOMED
  • Pluripotent cell lines and methods of use thereof
    Patent number: 8669048
    Abstract: Methods of generating cell lines with a sequence variation or copy number variation of a gene of interest, methods of use thereof, and cell lines with a sequence variation or copy number variation of a gene of interest are provided.
    Type: Grant
    Filed: June 24, 2009
    Date of Patent: March 11, 2014
    Assignee: Parkinson's Institute
    Inventors: Renee Ann Reijo Pera, J. William Langston, Birgitt Schüle, Theodore D. Palmer
  • Erythrocytes differentiated in vitro from nanofiber expanded CD133+ cells
    Patent number: 8669106
    Abstract: The invention provides, among other things, methods and systems for expanding CD133+ cells. The invention further provides methods and systems for increasing the blood flow to an ischemic tissue in a subject in need thereof, such as to ischemic myocardium. The invention further provides methods and systems for directing differentiation of expanded CD133+ cells. The invention further provides methods and systems for treating a subject with differentiated cells in a subject in need thereof.
    Type: Grant
    Filed: January 9, 2009
    Date of Patent: March 11, 2014
    Assignees: Arteriocyte Inc., Universite Pierre ET Marie Curie (Paris VI)
    Inventors: Ramasamy Sakthivel, Donald J. Brown, Hai-Quan Mao, Luc Douay, Vincent J. Pompili, Kevin McIntosh, Hiranmoy Das, Yukang Zhao
  • ANTI-KIT ANTIBODIES AND USES THEREOF
    Publication number: 20140065168
    Abstract: Provided herein, in one aspect, are antibodies that immunospecifically bind to a human KIT antigen comprising the fourth and/or fifth extracellular Ig-like domains (that is, D4 and/or D5 domains), polynucleotides comprising nucleotide sequences encoding such antibodies, and expression vectors and host cells for producing such antibodies. The antibodies can inhibit KIT activity, such as ligand-induced receptor phosphorylation. Also provided herein are kits and pharmaceutical compositions comprising antibodies that specifically bind to a KIT antigen, as well as methods of treating or managing a KIT-associated disorder or disease and methods of diagnosing a KIT-associated disorder or disease using the antibodies described herein.
    Type: Application
    Filed: July 24, 2013
    Publication date: March 6, 2014
    Applicant: KOLLTAN PHARMACEUTICALS, INC.
    Inventors: Yaron Hadari, Elizabeth M Mandel-Bausch, Francis Joseph Carr, Timothy David Jones, Laura Clare Alexandra Perry
  • CULTURE METHOD, GROUP OF MATURE ADIPOCYTES, DRUG SCREENING METHOD
    Publication number: 20140065659
    Abstract: In a culture method for adipocytes, seeded adipocytes are cultured while being allowed to adhere to a culture bottom surface (14) and walls (12) disposed perpendicularly to the culture bottom surface (14), without suspending the adipocytes in a culture solution, thereby obtaining mature adipocytes in which spherical lipid droplets having increased in size are generated within cells. This method uses a culture chamber (10) in which the walls (12) are formed perpendicular to the culture bottom surface (14). Spherical mature adipocytes containing spherical lipid droplets having increased in size within cells are cultured in a state where a cell or an aggregate of cells is allowed to adhere to two or more locations on the walls (12) and the culture bottom surface (14), thereby obtaining adipocytes having a shape similar to that of adipocytes in vivo.
    Type: Application
    Filed: March 16, 2012
    Publication date: March 6, 2014
    Applicant: Kuraray Co., Ltd.
    Inventors: Yoko Itchoda, Masaya Hosoda, Motohiro Fukuda, Masafumi Nagayama
  • Tendon Stem Cells
    Publication number: 20140065108
    Abstract: The invention relates to tendon stem cells useful for treating a variety of diseases and condition, including tendon repair and attachment of tendon to bone. The invention is also directed to treatment and/or inhibition of bone formation by use of biglycan and/or fibromodulin.
    Type: Application
    Filed: August 29, 2013
    Publication date: March 6, 2014
    Inventors: Marian F. Young, Yanming Bi, Songtao Shi
  • NEURAL STEM CELLS DERIVED FROM INDUCED PLURIPOTENT STEM CELLS
    Publication number: 20140065227
    Abstract: The present invention provides novel populations of neural stem cells derived from induced pluripotent stem cells, and methods for making and using the same.
    Type: Application
    Filed: June 18, 2013
    Publication date: March 6, 2014
    Inventors: Ole Isacson, Jan Pruzak, Marius Wernig, Rudolf Jaenisch
  • Simultaneous Delivery of Receptors and/or Co-Receptors for Growth Factor Stability and Activity
    Publication number: 20140066374
    Abstract: The compositions and methods of the present invention relate to the co-delivery of a molecule and a polypeptide to cells to improve the therapeutic efficacy of the molecules. In one embodiment of the invention, the invention may improve delivery of growth factors by co-delivering these growth factors with their receptors and co-receptors, such as syndecans. Co-delivery of growth factors with syndecans, for example, may protect growth factors from proteolysis, enhance their activity, and target the growth factors to the cell surface to facilitate growth factor signaling. This novel approach to growth factor therapy could be extended to other systems and growth factors enabling the enhancement of multiple signaling pathways to achieve a desired therapeutic outcome.
    Type: Application
    Filed: May 6, 2013
    Publication date: March 6, 2014
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Elazer R. Edelman, Aaron B. Baker
  • METHOD FOR REJUVENATING CELLS
    Publication number: 20140056860
    Abstract: The invention relates to a method for reprogramming cells from aged donors or senescent cells to pluripotent cells that have lost marks of senescence. In particular, the invention relates to an ex vivo method for preparing induced pluripotent stem cells (iPSCs) from a target cell population comprising cells from aged donors or senescent cells, said method comprising the steps of culturing said target cell population under appropriate conditions for reprogramming said cells into iPSCs, wherein said appropriate conditions comprises increasing expression in said target cells, of at least the following reprogramming factors: Oct4, Klf4, Sox2, c-Myc, Lin28 and, optionally Nanog.
    Type: Application
    Filed: April 10, 2012
    Publication date: February 27, 2014
    Applicants: UNIVERSITE MONTPELLIER II, UNIVERSITE MONTPELLIER I
    Inventors: Alex Prieur, Ollivier Milhavet, Jean-Marc Lemaitre, Laure Lapasset
  • METHODS FOR THE IDENTIFICATION OF AGENTS THAT INHIBIT MESENCHYMAL-LIKE TUMOR CELLS OR THEIR FORMATION
    Publication number: 20140057352
    Abstract: The present invention provides tumor cell preparations for use as models of the EMT process for use in the identification of anti-cancer agents, wherein said tumor cell preparations comprise cells of the epithelial tumor cell line H1650, which are stimulated by receptor ligands to induce EMT, or which have been engineered to inducibly express a protein that stimulates EMT. The present invention also provides methods of identifying potential anti-cancer agents by using such tumor cell preparations to identify agents that inhibit EMT, stimulate MET, or inhibit the growth of mesenchymal-like cells. Such agents should be particularly useful when used in conjunction with other anti-cancer drugs such as EGFR and IGF-1R kinase inhibitors, which appear to be less effective at inhibiting tumor cells that have undergone an EMT.
    Type: Application
    Filed: June 14, 2013
    Publication date: February 27, 2014
    Inventor: Julie Kan
  • ANTI-KIT ANTIBODIES AND USES THEREOF
    Publication number: 20140056905
    Abstract: Provided herein, in one aspect, are antibodies that immunospecifically bind to a human KIT antigen comprising the fourth and/or fifth extracellular Ig-like domains (that is, D4 and/or D5 domains), polynucleotides comprising nucleotide sequences encoding such antibodies, and expression vectors and host cells for producing such antibodies. The antibodies can inhibit KIT activity, such as ligand-induced receptor phosphorylation. Also provided herein are kits and pharmaceutical compositions comprising antibodies that specifically bind to a KIT antigen, as well as methods of treating or managing a KIT-mediated disorder or disease and methods of diagnosing a KIT-mediated disorder or disease using the antibodies described herein.
    Type: Application
    Filed: January 25, 2012
    Publication date: February 27, 2014
    Applicant: KOLLTAN PHARMACEUTICALS, INC.
    Inventors: Yaron Hadari, Elizabeth M. Mandel-Bausch, Susanne Radke, Joseph Schlessinger, Yoshihisa Suzuki
  • METHOD OF PRODUCING RETINAL PIGMENT EPITHELIAL CELL SHEET
    Publication number: 20140057281
    Abstract: The present invention provides a method of producing a cell sheet including the following steps (1) seeding and culturing retinal pigment epithelial cells on a collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and (2) degrading the collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells, and the like.
    Type: Application
    Filed: February 24, 2012
    Publication date: February 27, 2014
    Applicant: RIKEN
    Inventors: Masayo Takahashi, Satoshi Okamoto, Hiroyuki Kamao
  • Method for Generating Potent Dendritic Cells
    Publication number: 20140056845
    Abstract: Highly potent dendritic cells are generated in vivo or ex vivo by exposing precursor cells to an effective dose of IL-32.
    Type: Application
    Filed: April 16, 2012
    Publication date: February 27, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Robert Lazarus Modlin, Mirjam Schenk
  • Methods for Making Cells with an Extra-Embryonic Endodermal Precursor Phenotype
    Publication number: 20140057313
    Abstract: The invention is directed to methods for making cells in vitro that have an extra-embryonic endodermal precursor phenotype. The method involves culturing cells from blastocysts under specific defined conditions.
    Type: Application
    Filed: January 30, 2012
    Publication date: February 27, 2014
    Inventors: Catherine M. Verfaillie, Bert Binas
  • MAMMALIAN HAPLOID EMBRYONIC STEM CELLS
    Publication number: 20140057801
    Abstract: The invention relates to mammalian haploid embryonic stem cells and methods for the production of such stem cells. The inventions also relates to a cell culture and a cell line of mammalian haploid embryonic stem cells.
    Type: Application
    Filed: March 2, 2012
    Publication date: February 27, 2014
    Applicant: CAMBRIDGE ENTERPRISE LIMITED
    Inventors: Anton Wutz, Martin Leeb
  • NOVEL CARDIOMYOCYTE MARKER
    Publication number: 20140057287
    Abstract: The present invention provides a method for production or detection of a cardiomyocyte(s) and/or cardiac progenitor cell(s), comprises extracting a cardiomyocyte(s) and/or cardiac progenitor cell(s) from a cell population comprising cardiomyocytes and/or cardiac progenitor cells using as an index VCAM1 positivity.
    Type: Application
    Filed: April 2, 2012
    Publication date: February 27, 2014
    Applicant: KYOTO UNIVERSITY
    Inventors: Jun Yamashita, Hideki Uosaki
  • IMMUNOSUPPRESSIVE CELLS AND METHODS OF MAKING AND USING THEREOF
    Publication number: 20140056856
    Abstract: This invention relates to an immunosuppressive cell, and methods of obtaining the cell and using the cell. The immunosuppressive cell is obtained by culturing a precursor cell in a medium that contains a GRO chemokine.
    Type: Application
    Filed: June 4, 2013
    Publication date: February 27, 2014
    Applicant: National Health Research Institutes
    Inventors: Shu-Ching Hsu, Hsin-Wei Chen, Pele Choi- Sing Chong, Hsin-Yu Chen, Li-Tzu Wang
  • Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
    Patent number: 8658159
    Abstract: Methods for inducing, expanding, and/or generating alloantigen-specific regulatory T cells. Alloantigen-specific regulatory T cells can be induced, expanded, and/or generated from naive CD4+CD25? T cells by using CD40-activated B cells. The regulatory T cells can be human T cells. In one embodiment, the alloantigen-specific human regulatory T cells can be CD4highCD25+Foxp3+ regulatory T cells.
    Type: Grant
    Filed: April 30, 2009
    Date of Patent: February 25, 2014
    Assignees: Versitech Limited, The Board of Trustees of the Leland Stanford Junior University
    Inventors: Wenwei Tu, Yu-Lung Lau, David Bram Lewis
  • Myelinating oligodendrocyte precursor populations
    Patent number: 8658424
    Abstract: A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+/Sox1+ into a population of cells that are PDGFR?+.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: February 25, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Su-Chun Zhang, Baoyang Hu, Zhong-Wei Du
  • Method for promoting differentiation of pluripotent stem cells into cardiac muscle cells
    Patent number: 8658425
    Abstract: The present invention relates to a composition for promoting differentiation of pluripotent stem cells into cardiac muscle cells, and a method for inducing differentiation of pluripotent stem cells into cardiac muscle cells and a method for preparing cardiac muscle cells.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: February 25, 2014
    Assignee: Kyoto University
    Inventors: Norio Nakatsuji, Motonari Uesugi, Kouhei Yamada, Itsunari Minami, Tomomi Otsuji