Using Mechanical Means (e.g., Trituration, Etc.) Patents (Class 435/379)
  • Patent number: 11090646
    Abstract: A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: August 17, 2021
    Assignees: bioMerieux, Inc., BioFire Defense, LLC
    Inventors: Christopher S. Ronsick, Kirk Ririe, Mark S. Wilson, John D. Walsh, Ryan T. Hill
  • Patent number: 10925755
    Abstract: An osseointegratable prosthetic device suitable for implantation via a one-step surgical process includes a threaded insertion end, an externally accessible tool interface end, and an intermediately arranged scaffold portion defining recesses that permit ingrowth of skeletal tissue. Such recesses may include multiple longitudinal fins as well as transverse ribs, which may embody or be coated with porous material to promote bone ingrowth. A split flange portion includes discontinuous portions and may permit pretensioning of the device. The device may be fabricated as a unitary body structure from thermoplastic materials using techniques such as fused filament fabrication (a form of 3D printing). The osseointegratable prosthetic device enables one-step surgical implantation without requiring separately implantable fixture/sleeve and abutment portions according to conventional osseointegratable prosthetics. Methods for fabricating an osseointegratable prosthetic device are further provided.
    Type: Grant
    Filed: August 16, 2017
    Date of Patent: February 23, 2021
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Steven Lathers, Jeffrey La Belle
  • Patent number: 10508260
    Abstract: A cell mass dissociator for a pluripotent stem cell production system.
    Type: Grant
    Filed: March 11, 2019
    Date of Patent: December 17, 2019
    Assignee: I Peace, Inc.
    Inventors: Koji Tanabe, Brendan Kelly, Kenta Suto, Hidenori Shimoda, Ryoji Hiraide
  • Patent number: 10006705
    Abstract: Methods for treating bulk bone tissue are provided. The methods comprise contacting bulk bone tissue or frozen bulk bone tissue with an effective amount of a supercritical fluid so as to dry the bulk bone tissue. In various embodiments, the supercritical fluid destroys contaminants such that the frozen bulk bone tissue is 99.9% substantially pure. In various embodiments, contaminants removed from the frozen bulk bone tissue include lipids, viruses, bacteria, pyrogens, prions, micro-organisms and/or pathogens. In some embodiments, the supercritical fluid causes a 3 log reduction of bacteria within the frozen bulk bone tissue.
    Type: Grant
    Filed: February 9, 2015
    Date of Patent: June 26, 2018
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Guobao Wei, David R. Kaes, Susan J. Drapeau, Subhabrata Bhattacharyya
  • Patent number: 9610433
    Abstract: A percutaneous implant for reducing the risk of post-implant infection by reducing the likelihood of epidermal down-growth between the dermis of the skin and the implant. In one example, the implant comprises a barrier member between the epidermis and the dermis about the implant. In another example, a barrier is provided by removing a portion of the epidermis from the dermis about the implant.
    Type: Grant
    Filed: February 10, 2011
    Date of Patent: April 4, 2017
    Assignee: Cochlear Limited
    Inventor: Marcus Andersson
  • Patent number: 9078741
    Abstract: The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for grafting to a patient. In applying the method and/or in using the device, donor tissue is harvested, subjected to a cell dissociation treatment, cells suitable for grafting back to a patient are collected and dispersed in a solution that is suitable for immediate dispersion over the recipient graft site.
    Type: Grant
    Filed: September 1, 2011
    Date of Patent: July 14, 2015
    Assignee: Avita Medical Limited
    Inventors: Fiona M. Wood, Marie L. Stoner
  • Publication number: 20150132851
    Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
    Type: Application
    Filed: January 21, 2015
    Publication date: May 14, 2015
    Inventors: Rouzbeh R. Taghizadeh, John Meade
  • Patent number: 9023614
    Abstract: A method for rapidly collecting cells from a surface, such as a surface bearing fingerprints, for subsequent macromolecular analysis involves dispensing a predetermined amount of an aqueous solution onto the surface, and subjecting the aqueous solution to ultrasound waves to promote a detachment of the cells from the surface. Extraction of macromolecules such as DNA from the cells can be effected directly in the solution containing the collected cells by further subjecting the solution to ultrasound waves for a prescribed period of time to lyse the cells, and then extracting the DNA.
    Type: Grant
    Filed: July 9, 2004
    Date of Patent: May 5, 2015
    Inventor: Tofy Mussivand
  • Publication number: 20150118752
    Abstract: A portable apparatus useful for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue.
    Type: Application
    Filed: September 5, 2013
    Publication date: April 30, 2015
    Inventors: William W. Cimino, Ramon Llull, Adam J. Katz
  • Patent number: 9012222
    Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: April 21, 2015
    Assignee: Auxocell Laboratories, Inc.
    Inventor: Rouzbeh R. Taghizadeh
  • Publication number: 20150086514
    Abstract: The present invention provides a mesenchymal stem cell injection, a preparation method thereof, and application thereof in preparing diabetes mellitus drug. Mesenchymal stem cells used by the present invention come from the human umbilical cord and the human placenta. The ingredients of the mesenchymal stem cell injection are: mesenchymal stem cells, human albumin, low molecular weight heparin, a compound amino acid, vitamin C, and a solution medium. The solution medium is a compound electrolyte solution, glucose water, or a normal saline solution.
    Type: Application
    Filed: November 11, 2013
    Publication date: March 26, 2015
    Inventor: Zaimei JIA
  • Patent number: 8986678
    Abstract: The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: March 24, 2015
    Assignee: Centre Hospitalier Universitaire Vaudois
    Inventor: Lee Ann Laurent-Applegate
  • Patent number: 8986996
    Abstract: The present invention relates to a large-scale propagation and maintenance method of embryoid bodies generated from stem cells. More particularly, the present invention relates to a large-scale propagation and maintenance method of embryoid bodies retaining their intrinsic characteristics for a long period of time, comprising the step of continuously subculturing embryoid bodies that are primarily produced from embryonic stem cells or from induced pluripotent stem cells. According to the method of the present invention, after preparation of embryoid bodies from a limited number of stem cells, large-scale production and maintenance of embryoid bodies can be realized by a simple mechanical subculturing method without the continuous supply of stem cells.
    Type: Grant
    Filed: November 6, 2012
    Date of Patent: March 24, 2015
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Yee Sook Cho, Mi Young Son, Hyun Jin Kim
  • Publication number: 20150079679
    Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
    Type: Application
    Filed: November 19, 2014
    Publication date: March 19, 2015
    Inventors: Rouzbeh R. Taghizadeh, John Meade
  • Publication number: 20140377866
    Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
    Type: Application
    Filed: June 19, 2014
    Publication date: December 25, 2014
    Inventors: Jered Haun, Janice De Jesus, Elliot En-Yu Hui, Jiang Li
  • Publication number: 20140357506
    Abstract: The present invention provides for methods of isolating a stem cell or cell derived therefrom from a mixture of cells, for example, a mixture of adherent cells in culture. Cell isolation is achieved by the application of selective detachment forces.
    Type: Application
    Filed: June 21, 2012
    Publication date: December 4, 2014
    Applicant: Georgia Tech Research Licensing, Mail Code: 0415
    Inventors: Ankur Singh, Shalu Suri, Todd Christopher McDevitt, Hang Lu, Andres Jose Garcia
  • Patent number: 8846396
    Abstract: Method for the isolation, expansion and preservation of cardiac stem cells from human or animal tissue biopsy samples to be employed in cell transplantation and functional repair of the myocardium or other organs. Cells may also be used in gene therapy for treating cardiomyopathies, for treating ischemic heart diseases and for setting in vitro models to study drugs.
    Type: Grant
    Filed: August 22, 2011
    Date of Patent: September 30, 2014
    Assignee: Universita Degli Studi Di Roma “La Sapienza”
    Inventors: Alessandro Giacomello, Elisa Messina, Massimo Battaglia, Giacomo Frati
  • Publication number: 20140273221
    Abstract: Methods of cell culture using patterned SAM arrays are disclosed. Advantageously, the disclosed methods use SAM arrays presenting adhesion peptides to grow confluent monolayers that can invaginate to form an embryoid body.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Justin T. Koepsel, William L. Murphy, Sheeny K.L. Levengood, Stefan Zorn
  • Publication number: 20140255356
    Abstract: Methods of isolating stromal vascular fractions for use in human subjects from a tissue comprising a blood vessel using mechanical, optionally ultrasonic cavitation, enzymatic, and/or chemical methods are provided. These methods yield an increased number of the cells which constitute the stromal vascular fractions (about 10-fold greater) than methods which use collagenase to isolate these cells. The stromal vascular fraction may be isolated from any tissue that comprises blood vessels, for example, muscle and adipose tissue. Additionally, the tissue may be isolated obtained from nonliving sources and animals (e.g., postmortem).
    Type: Application
    Filed: March 6, 2014
    Publication date: September 11, 2014
    Inventor: Steven VICTOR
  • Patent number: 8828417
    Abstract: For an implantable device intended for use in the human body an in-vivo colonization with autologous cells is often desired. The devices, especially prosthetic devices like implant tissues, grafts, shunts, vessels, organs or a part of organs are commonly derived from animal or human origin and comprise a collagen-based tissue matrix. The invention proposes a coating deposited on a surface of the device and comprising the matrix protein CCN1 as an extracellular matrix-associated protein mediating cell adhesion or cell migration.
    Type: Grant
    Filed: July 8, 2008
    Date of Patent: September 9, 2014
    Assignee: Corlife OHG
    Inventors: Andres Hilfiker, Denise Hilfiker-Kleiner, Artur Lichtenberg
  • Patent number: 8822202
    Abstract: A unitary apparatus for isolating cells from adipose tissue including a lipid separation processor with a dispersing head equipped with a plurality of ports and a digestion chamber for dissociation of the constituent cells disposed in adipose tissue. The lipid separating apparatus is useful for the separation of lipids and adipocytes from a mixed cell population. A cell seeding chamber may be attached to the cell isolation apparatus. The components of the apparatus may be packaged in modular kit form.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: September 2, 2014
    Assignee: Ingeneron Incorporated
    Inventors: Ron Stubbers, Michael Coleman
  • Patent number: 8785193
    Abstract: The present disclosure provides cutting tools which include a handle coupled to a rotatable shaft having shallow grooves that extend substantially entirely across the surface of the rotatable shaft. The grooves define sharp cutting edges. In particular examples, the cutting edges are continuous, such as being defined by helical threads. The present disclosure also provides methods of dissecting a substrate of cultured cells. The substrate of cultured cells is separated into separated portions with a cutting tool. The cutting tool includes a rotatable shaft having a cutting blade which extends around the shaft. The cutting blade is rolled through the substrate to cut the substrate into portions. The portions are separated from one another to dissect the substrate.
    Type: Grant
    Filed: September 14, 2006
    Date of Patent: July 22, 2014
    Assignee: The United States of America, as represented by the Secretary of the Department of Health and Human Services
    Inventor: Soojung Shin
  • Publication number: 20140193377
    Abstract: The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.
    Type: Application
    Filed: July 10, 2012
    Publication date: July 10, 2014
    Applicant: Centre Hospitalier Universitaire Voudois (CHUV)
    Inventor: Lee Ann Laurent-Applegate
  • Publication number: 20140193852
    Abstract: Devices and methods are described for pre-processing tissue samples containing cells of interest for various therapeutic procedures. Fibrous material may be extracted from the tissue sample through a shredding and separating device, resulting in a non-fibrous sample containing the cells of interest, which may then be washed and centrifuged to remove fluids, oil, and contaminants. The sample may then be digested in a large-volume container, and a piston within the container may push the resulting liberated cell suspension through a cell concentrator such as a hollow-fiber separation module.
    Type: Application
    Filed: May 7, 2009
    Publication date: July 10, 2014
    Inventors: Erik Vossman, Shannon Iwami, Anthony Yang, Tom Cannon, Hyun Joon Paek
  • Patent number: 8772030
    Abstract: Method for the isolation, expansion and preservation of cardiac stem cells from human or animal tissue biopsy samples to be employed in cell transplantation and functional repair of the myocardium or other organs. Cells may also be used in gene therapy for treating cardiomyopathies, for treating ischemic heart diseases and for setting in vitro models to study drugs.
    Type: Grant
    Filed: September 26, 2011
    Date of Patent: July 8, 2014
    Assignee: Universita Degli Studi di Roma “La Sapienza”
    Inventors: Alessandro Giacomello, Elisa Messina, Massimo Battaglia, Giacomo Frati
  • Publication number: 20140186946
    Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.
    Type: Application
    Filed: March 15, 2013
    Publication date: July 3, 2014
    Applicant: GENERAL ELECTRIC COMPANY
    Inventor: General Electric Company
  • Patent number: 8753884
    Abstract: An improved method of producing differentiated progenitor cells comprising obtaining inner cell mass cells from a blastocyst and inducing differentiation of the inner cell mass cells to produce differentiated progenitor cells. The differentiated progenitor cells may be transfected such that there is an addition, deletion or alteration of a desired gene. The differentiated progenitor cells are useful in cell therapy and as a I source of cells for the production of tissues and organs for transplantation. Also provided is a method of producing a lineage-defective human embryonic stem cell.
    Type: Grant
    Filed: May 16, 2008
    Date of Patent: June 17, 2014
    Assignee: Advanced Cell Technology
    Inventors: Jose Cibelli, Michael D. West, Robert Lanza
  • Publication number: 20140162362
    Abstract: Disclosed are compositions and methods relating to growth of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) using non-simian cells. In a particular example, porcine alveolar macrophage cells are described as having a capability of supporting infectivity and reproduction by PRRSV. Cells and cell lines of the invention are disclosed in connection with applications relating to PRRS disease, including vaccine technologies.
    Type: Application
    Filed: February 14, 2014
    Publication date: June 12, 2014
    Applicant: The Board of Trustees of the University of Illinois
    Inventor: Federico A. ZUCKERMANN
  • Publication number: 20140056944
    Abstract: The present invention includes a method and device for the isolation of lymphocytes, particularly splenic lymphocytes, for administration to patients. In particular for patients undergoing a splenectomy, administration of splenic lymphocytes may increase a patient's immunity and reduce the likelihood of post-splenectomy infections and other complications.
    Type: Application
    Filed: August 22, 2013
    Publication date: February 27, 2014
    Applicant: Children's Hospital of Orange County
    Inventor: Mustafa Kabeer
  • Patent number: 8652835
    Abstract: A device for mechanically decollating cells from a cell composite, particularly a shear rotor, a cell isolation unit including such a shear rotor and a method for decollating cells from a cell composite. The aforementioned device includes a rotor having a rotor wall, which is concentrically arranged in a receptacle. A rotor seat is connected to a motor and to which the rotor can be fixed in a detachable manner. A rotational movement of the motor can be transmitted to the rotor by means of the rotor seat. The rotor tapers longitudinally towards the bottom of the receptacle so that different circumferential speeds of the rotor can be transmitted to a liquid sample in the receptacle via the rotor wall.
    Type: Grant
    Filed: January 31, 2008
    Date of Patent: February 18, 2014
    Assignee: Eppendorf AG
    Inventors: Holger Link, Alexander Papra, Michael Blumentritt, Vinh Duong
  • Publication number: 20140010790
    Abstract: The present invention relates material based on microbial cellulose that is useful in culturing and transferring cells as well as delivering drugs. The material comprises cellulose nanofibers and/or derivatives thereof, based on microbial cellulose, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.
    Type: Application
    Filed: October 26, 2011
    Publication date: January 9, 2014
    Applicant: UPM-KYMMENE CORPORATION
    Inventors: Marjo Yliperttula, Patrick Laurén, Madhushree Bhattacharya, Yanru Lou, Antti Loukkanen
  • Publication number: 20130302890
    Abstract: Methods for isolating and culturing stem cells, making tissue matrix, making matrix infused with stem cells, and methods of stem cell therapy are provided.
    Type: Application
    Filed: May 8, 2013
    Publication date: November 14, 2013
    Inventor: Raymond MOUZANNAR
  • Publication number: 20130295673
    Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
    Type: Application
    Filed: July 1, 2013
    Publication date: November 7, 2013
    Inventors: Rouzbeh R. Taghizadeh, John Meade
  • Publication number: 20130225422
    Abstract: Disclosed herein is a system and method for making allele calls, and for determining the ploidy state, in one or a small set of cells, or where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed and the haplotypes are determined using expected similarities between the target genome and the knowledge of the genomes of genetically related individuals. In one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the genetic data from both parents, and possibly one or more sperm and/or sibling embryos. In another embodiment, the chromosome copy number can be determined using the same input data. In another embodiment, these determinations are made for embryo selection during IVF, for non-invasive prenatal diagnosis, or for making phenotypic predictions.
    Type: Application
    Filed: March 18, 2013
    Publication date: August 29, 2013
    Applicant: Natera, Inc.
    Inventors: Matthew Rabinowitz, George Gemelos, Milena Banjevic, Allison Ryan, Joshua Sweetkind-Singer
  • Publication number: 20130224856
    Abstract: The present invention relates to a large-scale propagation and maintenance method of embryoid bodies generated from stem cells. More particularly, the present invention relates to a large-scale propagation and maintenance method of embryoid bodies retaining their intrinsic characteristics for a long period of time, comprising the step of continuously subculturing embryoid bodies that are primarily produced from embryonic stem cells or from induced pluripotent stem cells. According to the method of the present invention, after preparation of embryoid bodies from a limited number of stem cells, large-scale production and maintenance of embryoid bodies can be realized by a simple mechanical subculturing method without the continuous supply of stem cells.
    Type: Application
    Filed: November 6, 2012
    Publication date: August 29, 2013
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventor: Korea Research Institute of Bioscience and Biotec
  • Publication number: 20130216505
    Abstract: Various cells, stem cells, and stem cell components, including associated methods of generating and using such cells are provided. In one aspect, for example, an isolated cell that is capable of self-renewal and culture expansion and is obtained from a subepithelial layer of a mammalian umbilical cord tissue. Such an isolated cell expresses at least three cell markers selected from CD29, CD73, CD90, CD166, SSEA4, CD9, CD44, CD146, or CD105, and does not express at least three cell markers selected from CD45, CD34, CD14, CD79, CD106, CD86, CD80, CD19, CD117, Stro-1, or HLA-DR.
    Type: Application
    Filed: December 31, 2012
    Publication date: August 22, 2013
    Inventor: Amit Patel
  • Publication number: 20130210140
    Abstract: A carrier for growing stem cells is provided, the carrier comprises a substrate comprising one or more outer surfaces; and a hydrophilic, water soluble coating material disposed and dried on one or more of the outer surfaces. The carrier comprises one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of culturing stem cells and stromal cells using the same carrier are also provided.
    Type: Application
    Filed: March 15, 2013
    Publication date: August 15, 2013
    Applicant: GENERAL ELECTRIC COMPANY
    Inventor: GENERAL ELECTRIC COMPANY
  • Publication number: 20130196369
    Abstract: The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.
    Type: Application
    Filed: January 31, 2013
    Publication date: August 1, 2013
    Applicants: UNIVERSITY OF SOUTHERN CALIFORNIA, THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, UNIVERSITY OF SOUTHERN CALIFORNIA
  • Publication number: 20130183273
    Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.
    Type: Application
    Filed: January 29, 2013
    Publication date: July 18, 2013
    Applicant: Auxocell Laboratories, Inc.
    Inventor: Auxocell Laboratories, Inc.
  • Patent number: 8450105
    Abstract: A process for making a gel comprising combining a silanol species comprising at least two silanol groups per molecule and a hydrophilic hydroxyl species comprising at least two hydroxyl groups per molecule. The gel is capable of being converted to a liquid by application of a mechanical shear force and the liquid is capable of being converted to the gel in the absence of the mechanical shear force.
    Type: Grant
    Filed: May 4, 2007
    Date of Patent: May 28, 2013
    Assignee: Agency for Science, Technology and Research
    Inventors: Jackie Y. Ying, Shona Pek, Andrew C A Wan
  • Publication number: 20130121972
    Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.
    Type: Application
    Filed: June 1, 2011
    Publication date: May 16, 2013
    Inventor: Rouzbeh R. Taghizadeh
  • Publication number: 20130115697
    Abstract: This document describes methods and an apparatus for recovery of cells from a tissue sample. In some embodiments, at least two rounds of acceleration and deceleration are performed.
    Type: Application
    Filed: November 3, 2011
    Publication date: May 9, 2013
    Inventors: Eckhard U. Alt, Michael E. Coleman, Ron Stubbers
  • Publication number: 20130040358
    Abstract: Disclosed is a device useful for preserving tissue samples after extraction from human or animal patients. The device includes a circular blade for taking a tissue sample, the blade being coupled to a sealing cap which mates to one end of a container. A retrieval port is also disclosed for extracting fluids from the container. Methods are also disclosed by which tissue samples may be taken and prepared for storage, processing, culture, or other analysis.
    Type: Application
    Filed: August 9, 2012
    Publication date: February 14, 2013
    Inventors: Erik J. Woods, Shelly J. Zacharias
  • Patent number: 8372644
    Abstract: A method for growing adult cells includes harvesting a tissue sample from a subject and breaking the tissue sample into fragments. The fragments are placed into a culture vessel, and at least some of the fragments are induced to adhere to the culture vessel. The fragments are supplied with nutrients so that adult cells contained therein divide and grow.
    Type: Grant
    Filed: February 6, 2007
    Date of Patent: February 12, 2013
    Assignee: Transplantation Limited
    Inventors: Patrick J. Casey, Richard Fry, Kerri Fry
  • Publication number: 20120315702
    Abstract: Embodiments are directed to devices and methods for processing, cultivating or otherwise manipulating cell cultures which may be disposed on a flat or substantially flat surface such as cell culture substrate material. Devices and methods are disclosed for dividing a cell culture layer into divided portions, including isolated divided portions, that may then be transferred from the cell culture to a new location. For some embodiments, the divided portions may be transferred to a new cell culture support substrate in order to continue to grow and cultivate the cell line.
    Type: Application
    Filed: June 18, 2012
    Publication date: December 13, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan CHESNUT, Robert DEES, Soojung SHIN
  • Patent number: 8329468
    Abstract: An innovative method of collecting cord blood stem cells from an isolated mammalian non-exsanguinated or partially exsanguinated placenta by placental perfusion is described and also an easy method for safe long duration cold storage of the placenta. Placental perfusion can include perfusing the isolated placenta with a pulsatile flow of perfusion solution, for example, using a pulsatile or peristaltic pump or device. The stem cells can then be isolated from the perfusate. Significantly increased amounts of CD133+ stem cells can be collected from the perfusate. The perfusion solution can include an anticoagulant. The isolated mammalian placenta need not be treated with an anticoagulant prior to perfusing. The isolated placenta can be free from an anticoagulant prior to perfusing.
    Type: Grant
    Filed: November 10, 2008
    Date of Patent: December 11, 2012
    Assignee: Cord Blood Science Inc.
    Inventor: Naoko Takebe
  • Patent number: 8304237
    Abstract: The invention relates to a method for cultivating biologic cells (1), wherein cells (1) are grown on a substrate (10) having a plurality of substrate openings (11), and wherein cell aggregates (2) are formed, including groups of cells (1) that span the substrate openings (11). A separation of the cell aggregates (2) from the substrate (10) by extracting the cell aggregates (2) from the substrate openings (11) can be provided. The invention further relates to a cell-cultivating device (100), including a substrate (10) having a plurality of substrate openings (11) and cell aggregates (2) including groups of cells (1) that span the substrate openings (11). The cell aggregates (2) are particularly used in high throughput tests with biologically active substances or in methods of tissue cultivation.
    Type: Grant
    Filed: June 13, 2008
    Date of Patent: November 6, 2012
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forschung e.V.
    Inventors: Charli Kruse, Sandra Danner
  • Publication number: 20120264213
    Abstract: A lipoaspirate collection device to aid in the collection and processing of human tissue and fluid obtained during liposuction for use in point-of-care cell therapy. The collection device includes a collection body and a collection cap. The collection cap may have a fluid port, a lipoaspirate port, a vacuum port, and a relief valve. Within the central cavity of the collection device, a cone shaped may be positioned such that the apex of the cone is positioned underneath the lipoaspirate inlet through which the lipoaspirate fluid and tissue are introduced.
    Type: Application
    Filed: October 4, 2011
    Publication date: October 18, 2012
    Inventors: Ted Sand, Kevin Dunworth, Greg Forman
  • Patent number: 8273526
    Abstract: The present invention provides methods of cryopreserving stem and progenitor cells in a mammalian placenta; and methods of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta. Cells obtained by carrying out the methods can be used in a variety of therapeutic applications.
    Type: Grant
    Filed: June 13, 2008
    Date of Patent: September 25, 2012
    Assignee: Children's Hospital & Research Center at Oakland
    Inventors: Frans A. Kuypers, Vladimir B. Serikov
  • Publication number: 20120230963
    Abstract: Methods for producing a population of human-derived glial restricted progenitor cells (GRPs) with decreased potentially unintended or undesired cellular phenotypes and/or decreased standard deviation in the cells of the population are provided. Also provided are antibody panels and gene expression profiles to characterize GRPs and a method for its use in characterizing GRP cells. In addition methods for use of these GRP cells to generate astrocytes and/or oligodendrocytes, to re-myelinate neurons and to treat glial cell related and other neurodegenerative diseases or disorders or injuries or damage to the nervous system are provided. A method to manufacture neural cells depleted of A2B5 positive cells is also provided.
    Type: Application
    Filed: November 9, 2010
    Publication date: September 13, 2012
    Applicant: Q Therapeutics, Inc.
    Inventors: Robert Sandrock, James T. Campanelli, Deborah A. Eppstein