Tobacco Cell Or Cell Line, Per Se Patents (Class 435/414)
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Publication number: 20080182753Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from Oryza sativa and useful for expressing transgenes in plants, in particular root tissues. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.Type: ApplicationFiled: December 5, 2006Publication date: July 31, 2008Inventors: Peter Hajdukiewicz, Qi Wang, Wei Wu
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Publication number: 20080178337Abstract: The invention relates to the soybean variety designated 4953710. Provided by the invention are the seeds, plants and derivatives of the soybean variety 4953710. Also provided by the invention are tissue cultures of the soybean variety 4953710 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 4953710 with itself or another soybean variety and plants produced by such methods.Type: ApplicationFiled: January 20, 2007Publication date: July 24, 2008Inventor: Nancy Sebern
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Publication number: 20080172762Abstract: The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.Type: ApplicationFiled: December 10, 2007Publication date: July 17, 2008Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: David Cerf, Ruth Cong, Michael Freeman, Kevin McBride, Takashi Yamamoto
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Publication number: 20080127366Abstract: The present invention relates to a gene being capable of modifying resistance against a heavy metal or salt, or accumulation properties, a recombination vector including the genes, and a transformant using the recombination vector. A gene having heavy metal resistance and accumulation properties includes a sequence encoding a transmembrane protein having five times repeated similar four transmembrane domains.Type: ApplicationFiled: August 2, 2007Publication date: May 29, 2008Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventors: Young-Sook Lee, Won-Yong Song, Do-Young Kim, Dong-Hwan Shim, Byeong-Wook Jeon
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Publication number: 20080076177Abstract: The inventions is drawn towards vectors and methods useful for preparing genetically transformed plant cells that express immunogens from pathogenic organisms which are used to produce immunoprotective particles useful in vaccine preparations. The invention includes plant optimized genes that encode the HN protein of Newcastle Disease Virus. The invention also relates to methods of producing an antigen in a transgenic plant.Type: ApplicationFiled: June 8, 2006Publication date: March 27, 2008Inventors: Guy A. Cardineau, Hugh Stanley Mason, Joyce M. VanEck, Dwayne D. Kirk, Amanda Maree Walmsley
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Patent number: 7294758Abstract: The subject invention pertains to materials and methods for producing plants that are resistant to infection by geminiviruses and other related viruses. Methods of the invention comprise transforming a plant with a polynucleotide wherein when the polynucleotide is expressed in the plant, the transformed plant exhibits resistance to plant viral infections. Exemplified herein is the use of a polynucleotide encoding a Rep protein derived from tomato mottle geminivirus. The methods of the invention can be used to provide resistance to viral infection in plants such as tomato and tobacco. The present invention also concerns transformed and transgenic plants in plant tissue that express a polynucleotide encoding a plant virus Rep protein, or a fragment or variant thereof.Type: GrantFiled: May 23, 2005Date of Patent: November 13, 2007Assignee: University of Florida Research Foundation, Inc.Inventors: Jane E. Polston, Ernest Hiebert, Ahmed M. Abouzid, Wayne B. Hunter
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Patent number: 7273727Abstract: The invention relates to compounds which contain an antigen binding region which is bound to at least one enzyme which is able to metabolize a compound (prodrug) which has little or no cytotoxicity to a cytotoxic compound (drug), where the antigen binding region is composed of a single polypeptide chain. It is advantageous for covalently bonded carbohydrates to be present on the polypeptide chain.Type: GrantFiled: May 23, 2005Date of Patent: September 25, 2007Assignee: Behrinwerke AktiengesellschaftInventors: Mathias Gehrmann, Gerhard Seeman, Klaus Bosslet, Jorg Czech
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Patent number: 7232677Abstract: The invention provides a purified or recombinant phytase enzyme (SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.Type: GrantFiled: February 5, 2001Date of Patent: June 19, 2007Assignee: Diversa CorporationInventors: Jay M. Short, Keith A. Kretz
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Patent number: 7205150Abstract: A method for obtaining a transgenic plant that over-expresses a soluble isoform AGPPase enzyme. The method includes a step of transforming a plant with a vector comprising a polynucleotide of SEQ ID NO: 7 linked to a promoter that promotes expression of the polynucleotide in the plant whereby to form the transgenic plant. The transgenic plant has a reduced starch content and a higher resistance to salinity than the plant before the transforming step.Type: GrantFiled: February 1, 2001Date of Patent: April 17, 2007Assignees: Universidad Publica de Navarra, JCR Pharmaceuticals Co., Ltd.Inventors: Javier Pozueta Romero, Edurne Baroja Fernandez, Aitor Zandueta Criado, Milagros Rodriguez Lopez, Francisco Jose Muñoz Perez
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Patent number: 7118914Abstract: The present invention relates to a method for producing target protein in plant cell culture, more particularly to the method for producing target protein comprising the steps of i) constructing vector containing SWPA2 promoter and gene for the target protein, ii) introducing said vector into Agrobacterium, iii) transforming said Agrobacterium into plant cell, and iv) mass-producing target protein using said transformed plant cells. The method of the present invention can mass-produce target proteins such as human lactoferrin. Moreover, the transformed plant can also be used to make health foods when medicinal plant is used as a host cell.Type: GrantFiled: March 25, 2003Date of Patent: October 10, 2006Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Haeng-Soon Lee, Sang-Soo Kwak, Suk-Yoon Kwon, Dae-Yeul Yu, Jae Whune Kim, Ok-Sun Lee
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Patent number: 7091031Abstract: A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.Type: GrantFiled: August 9, 2005Date of Patent: August 15, 2006Assignee: E. I. du Pont de Nemours and CompanyInventors: Qiong Cheng, Luan Tao, Natalia Sedkova
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Patent number: 7049486Abstract: The present invention is to provide a method which comprises providing a plant with characters of a repressor and operator both constituting a gene expression inducing system with an actinomycete autogenous regulatory factor as an inducer by gene transfer and administering the actinomycete autogenous regulatory factor to the transformed plant to thereby induce the expression of a gene placed under the control of the operator at a site of administration of the actinomycete autogenous regulatory factor. This method makes it possible to cause expression of a desired gene at a desired time and site, thus enabling even the production, in a plant, of a metabolite otherwise disadvantageous to the growth of the plant. It is also useful in preventing transformant plants from spreading through the environment by controlling the fertility thereof.Type: GrantFiled: June 15, 2001Date of Patent: May 23, 2006Assignee: Kaneka CorporationInventors: Atsuhiko Shinmyo, Kou Kato, Yasuhiro Yamada, Takuya Nihira, Takuya Shindo
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Patent number: 6881877Abstract: The invention provides a process for producing trehalose in plant cells capable of producing trehalase by growing plant cells having the genetic information required for the production of trehalose and trehalase, or cultivating a plant or a part thereof comprising such plant cells, characterized in that said plant cells are grown, or said plant or a part thereof, is cultivated in the presence of a trehalase inhibitor.Type: GrantFiled: January 7, 1997Date of Patent: April 19, 2005Assignee: Mogen International NVInventors: Oscar Johannes Maria Goddijn, Teunis Cornelis Verwoerd, Ronny Wilhelmus Hermanus Henrika Krutwagen, Eline Voogd
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Patent number: 6818803Abstract: Transgenic plants which express cellulose-degrading enzymes, methods to make the transgenic plants, and methods to use the cellulose-degrading enzymes produced by the transgenic plants are disclosed.Type: GrantFiled: August 12, 1999Date of Patent: November 16, 2004Assignee: Wisconsin Alumni Research FoundationInventors: Sandra Austin-Phillips, Richard R. Burgess, Thomas L. German, Thomas Ziegelhoffer
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Patent number: 6759570Abstract: The present invention refers to an isolated nucleic acid molecule for expression of a gene in seeds, having promoter activity, comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, a first sequence at least 70% homologous to SEQ ID NO:1, with the functionality of SEQ ID NO:1, a second sequence at least 70% homologous to the sequence complementary to SEQ ID NO:1, with the functionality of SEQ ID NO:1, and fragments thereof, with the functionality of SEQ ID NO:1; to chimeric genes, constructs, vectors, expression cassettes, host cells and transgenic plants comprising said sequences; as well as to a method for expression of a gene specifically in seeds or seed parts using one of the mentioned sequences or chimeric genes comprising said sequence s and to a method for obtaining substances through the transferring of the mentioned chimeric genes to a plant and expressing said chimeric gene.Type: GrantFiled: August 22, 2000Date of Patent: July 6, 2004Assignee: Consejo Superior de Investigaciones CientificasInventors: Maria Pilar Prieto-Dapena, María Concepción Almoguera Antolinez, Juán Bautista Jordano Fraga
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Patent number: 6734343Abstract: An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having a flavanone-7-O-glucoside-2″-O-rhamnosyl-transferase catalytic activity and its uses.Type: GrantFiled: July 20, 2001Date of Patent: May 11, 2004Assignees: Yeda Research and Development Co. Ltd., Agricultural Research Organization, The Volcani CenterInventors: Jonathan Gressel, Yoram Eyal, Robert Fluhr
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Publication number: 20040060087Abstract: The present invention relates to novel interspecific Nicotiana excelsior×N. benthamiana hybrid seeds and plants and to a method of producing interspecific Nicotiana hybrids having enhanced properties for biomass and the production of recombinant proteins using a viral vector system.Type: ApplicationFiled: September 18, 2003Publication date: March 25, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventor: Wayne P. Fitzmaurice
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Publication number: 20030219897Abstract: The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region.Type: ApplicationFiled: March 20, 2002Publication date: November 27, 2003Inventors: Monto Hiroshi Kumagai, Guy Richard Della-Cioppa, Jonathan Donson, Damon Alan Harvey
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Publication number: 20030192078Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis, B.t. entomocidus, 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.Type: ApplicationFiled: March 19, 2002Publication date: October 9, 2003Inventors: David A. Fischhoff, Frederick J. Perlak
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Patent number: 6608241Abstract: The invention involves recombinant, double-stranded DNA that contains a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus, a DNA sequence that causes the production of an RNA sequence encoding the coat protein of said plant virus, and a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence; which double-stranded DNA can be used in a method for genetically transforming plants to produce genetically transformed plant cells and plants that are resistant to virus infection.Type: GrantFiled: October 9, 1986Date of Patent: August 19, 2003Assignees: Monsanto Technology LLC, Washington UniversityInventors: Roger N. Beachy, Robert T. Fraley, Stephen G. Rogers
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Patent number: 6605754Abstract: The invention relates to a chemically-inducible plant gene expression cassette and to plant cells transformed therewith. The expression cassette is suitable for use in any dicotyledonous or monocotyledonous plant. It comprises a first promoter operatively linked to a regulator sequence which encodes a regulator protein, and an inducible promoter operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene. The regulator sequence may be derived from the alcR gene, and the inducible promoter may be derived from the alcA gene promoter (both obtainable from Aspergillus nidulans). A chimeric promoter comprising an upstream regulatory region and a heterologous downstream transcription initiation region is also disclosed. The regulatory sequence may be derived from the alcA gene promoter.Type: GrantFiled: March 17, 1998Date of Patent: August 12, 2003Assignee: Syngenta LimitedInventors: Mark X. Caddick, Andrew J. Greenland, Kay V. Riddell, Wolfgang W. Schuch, Arthur B. Tomsett
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Patent number: 6583338Abstract: The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.Type: GrantFiled: May 7, 2001Date of Patent: June 24, 2003Assignee: DeKalb Genetics CorporationInventors: David McElroy, Alan L. Kriz, Emil M. Orozco, Jr., Matt Griffor
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Patent number: 6573431Abstract: The invention concerns the use of recombinant nucleotides sequences containing cDNA coding for a preduodenal lipase, or any sequence derived from this cDNA, for transforming plant cells in order to obtain recombinant preduodenal lipase or polypeptide derivatives. The invention also concerns the use of genetically modified plants or parts thereof, or extracts of these plants or the use of recombinant preduodenal lipase or résultant polypeptide derivatives in the field of foodstuffs, or for producing medicaments, or in industry.Type: GrantFiled: July 2, 1999Date of Patent: June 3, 2003Assignees: Biochem S.A., Jouveinal S.A.Inventors: Philippe Lenee, Véronique Gruber, Sylvie Baudino, Bertrand Merot, Claude Benicourt, Claire Cudrey
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Use of bacterial acetate kinase and their genes for protection of plants against different pathogens
Patent number: 6476293Abstract: An isolated gene fragment that encodes for acetate kinase, which confers disease resistance in plants is disclosed. The gene can be cloned into an expression vector to produce a recombinant DNA expression system suitable for insertion into cells to form a transgenic plant transformed with the gene fragment. A method for conferring disease resistance in plants that consists of growing plant host cells transformed with the expression system and expressing the gene conferring disease resistance to impart such resistance to host cells is also disclosed.Type: GrantFiled: October 1, 1999Date of Patent: November 5, 2002Assignee: University of Kentucky Research FoundationInventors: Arthur G. Hunt, Glenn B. Collins, Christopher Lawrence, Qingshun Li, Santanu Dasgupta -
Publication number: 20020160410Abstract: Methods for amplification of nucleic acids in cells are provided. Also provided are cells that contain the the nucleic acids.Type: ApplicationFiled: April 17, 2002Publication date: October 31, 2002Inventors: Gyula Hadlaczky, Aladar A. Szalay
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Patent number: 6472587Abstract: Process for production of herbicide-tolerant plants by expressing an exogenous herbicide-binding polypeptide in plants or plant organs. The invention furthermore relates to the use of the corresponding nucleic acids which encode a polypeptide, an antibody or parts of an antibody with herbicide-binding properties in transgenic plants, and the thus transformed plant itself.Type: GrantFiled: September 21, 1999Date of Patent: October 29, 2002Assignee: BASF AktiengesellschaftInventors: Jens Lerchl, Achim Möller, Ralf-Michael Schmidt, Helmut Schiffer, Udo Rabe, Udo Conrad
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Publication number: 20020155605Abstract: The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region.Type: ApplicationFiled: March 20, 2002Publication date: October 24, 2002Inventors: Monto Hiroshi Kumagai, Guy Richard Della-Cioppa, Jonathan Donson, Damon Alan Harvey
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Patent number: 6448070Abstract: A fusion capsid protein comprising a plant virus capsid protein fused to an antigenic polypeptide is used as a molecule for presentation of that polypeptide to the immune system of an animal such as a human. The plant virus capsid protein is that of an alfalfa mosaic virus (AlMV) or ilarvirus.Type: GrantFiled: February 25, 1999Date of Patent: September 10, 2002Assignee: Thomas Jefferson UniversityInventors: Hilary Koprowski, Vidadi Yusibov, Douglas Craig Hooper, Anna Modelska
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Patent number: 6448476Abstract: The invention relates in general to plants, plant cells, methods of making, and methods of using plants and plant cells transformed to contain a DNA sequence encoding an AMPA-N-acetyltransferase, and to plants and plant cells exhibiting resistance to AMPA in an amount which inhibits the growth of a plant or plant cell lacking a sequence encoding an AMPA-N-acetyltransferase.Type: GrantFiled: November 16, 1999Date of Patent: September 10, 2002Assignee: Monsanto Technology LLCInventor: Gerard F. Barry
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Patent number: 6441273Abstract: The present invention relates to the isolation of two DNA promoters from a coffee plant. The isolated promoters, one inducible and one constitutive, are capable of inducing the expression of a second DNA operably linked to the promoter. The present invention also relates to host cells, expression systems and transgenic plants containing the promoters of the invention.Type: GrantFiled: April 7, 2000Date of Patent: August 27, 2002Assignee: Cornell Research Foundation, Inc.Inventors: Herbert S. Aldwinckle, Alvaro L. Gaitan
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Patent number: 6433251Abstract: Nucleic acid molecules that encode a plant promoter involved in photoperiodism and circadian rhythms are disclosed. These molecules may be introduced into plants in order to alter the photoperiodic and/or circadian clock-based gene expression of the plants.Type: GrantFiled: February 24, 2000Date of Patent: August 13, 2002Assignee: The State of Oregon acting by and through the State Board of Higher Education on behalf of the University of OregonInventors: D. Ry Wagner, Karen A. Hicks, Michelle Z. Spence, Henriette Foss, Xing Liang Liu, Michael F. Covington
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Patent number: 6429357Abstract: The current invention provides regulatory regions from the rice actin 2 gene. In particular, the current invention provides the rice actin 2 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the rice actin 2 intron and/or promoter directly by genetic transformation, as well as by plant breeding methods. The actin 2 sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.Type: GrantFiled: May 14, 1999Date of Patent: August 6, 2002Assignees: Dekalb Genetics Corp., Cornell Research Foundation, Inc.Inventors: David McElroy, Ray Wu
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Patent number: 6392121Abstract: A gene amplification system based on plant viral genetic elements dramatically increases foreign protein production in plants. A safer and more economical production system for vaccines and antibodies in recombinant plants grown using agricultural practice is described. The high-level expression system uses the replicative process of a plant mastrevirus, exemplified by bean yellow dwarf virus (BeYDV). The expression system is preferably inducible to avoid interference with plant growth and development. Developmental cues, such as fruit ripening, are employed to trigger expression of the foreign protein using a tissue-specific promoter. A single, stably integrated expression cassette for foreign protein is replicated extrachromosomally in ripening fruit, forming hundreds of transcriptionally competent copies. Preferred plant hosts include tomato as a model system and soybean for production of large quantities of protein at high total protein levels.Type: GrantFiled: October 7, 1999Date of Patent: May 21, 2002Assignee: Boyce Thompson Institute for Plant ResearchInventors: Hugh S. Mason, Kenneth E. Palmer, Kathleen L. Hefferon, Tsafrir S. Mor, Charles Arntzen
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Patent number: 6376745Abstract: The present invention is drawn to a recombinant nucleic acid having (a) a transcriptional promoter; (b) a first plant-expressible structural gene linked to the transcriptional promoter, (c) a cDNA sequence element having an internal ribosome entry site of tobamovirus moving protein (MP) gene (IRESmp) which is located 3′ to the first plant-expressible structural gene, and (d) a second plant-expressible gene located 3′ to the (IRESmp) such that the second gene is placed under the translational control of (IRESmp); wherein the first plant-expressible gene, (IRESmp) and the second plant-expressible gene are transcribed under the action of the transcriptional promoter to give a primary transcript, and the first plant expressible gene of the primary transcript is able to translate by ribosome scanning mechanism and the second plant expressible gene of the primary transcript is capable of translation under the action of (IRESMP).Type: GrantFiled: December 16, 1999Date of Patent: April 23, 2002Inventors: Joseph Atabekov, Timo Korpela, Yurii Dorokhov, Peter Ivanov, Maxim Skulachev, Nina Rodionova, Olga Karpova
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Patent number: 6342655Abstract: CP genes of CMV strains V27, V33, V34, and A35 (CMV-V27, CMV-V33, CMV-V34, and CMV-A35 respectively) are provided.Type: GrantFiled: July 14, 2000Date of Patent: January 29, 2002Assignee: Seminis Vegetable Seeds, Inc.Inventors: Maury L. Boeshore, J. Russell McMaster, David M. Tricoli, John F. Reynolds, Kim J. Carney
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Patent number: 6338850Abstract: A method is provided for expressing a mammalian antigen in transformed plants to provide a source of plant material for oral or enteral administration to a mammal to produce tolerance to the antigen.Type: GrantFiled: May 21, 1996Date of Patent: January 15, 2002Assignee: London Health Sciences CentreInventors: Anthony M. Jevnikar, Shengwu Ma, Calvin R. Stiller
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Publication number: 20010055788Abstract: A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.Type: ApplicationFiled: February 5, 2001Publication date: December 27, 2001Applicant: Diversa CorporationInventors: Jay M. Short, Keith A. Kretz
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Patent number: 6320101Abstract: A method of enhancing inorganic carbon fixation by a photosynthetic organism. The method is effected by transforming cells of the photosynthetic organism with an expressible polynucleotide encoding a polypeptide having a bicarbonate transporter activity. Preferably, the polynucleotide further includes a plant promoter. Sequences and constructs for implementing the method are also described.Type: GrantFiled: June 14, 1999Date of Patent: November 20, 2001Assignee: Yissum Research Development Company of the Hebrew University of JerusalemInventors: Aaron Kaplan, Judy Lieman-Hurwitz, Daniella Schatz, Ron Mittler, Michal Ronen-Tarazi, David J. Bonfil
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Patent number: 6284952Abstract: The present invention provides transgenic plants and plant cells thereof which have been transformed with the soybean calmodulin isoform (SCaM5) gene to exhibit greatly enhanced resistance to a wide spectrum of plant pathogens. The present invention also provides the expression vector containing SCaM5 gene and to host cells into which the gene in the expression vector has been introduced to plant pathogens-resistant plants. Transgenic plants expressing a heterologous SCaM5 gene show increased resistance to fungi, bacteria and viruses which normally infect the plants.Type: GrantFiled: January 29, 1999Date of Patent: September 4, 2001Assignee: Korea Kumho Petrochemical Co., Ltd.Inventors: Won Do Heo, Moo Je Cho, Pill-Soon Song, Chang Ho Chung
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Patent number: 6245974Abstract: Matrix attachment regions isolated from a higher plants, and DNA constructs and vectors containing such matrix attachment regions, are described. A method of identifying matrix attachment regions is provided.Type: GrantFiled: July 24, 1998Date of Patent: June 12, 2001Assignee: North Carolina State UniversityInventors: Susan Michalowski, Steven Spiker
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Patent number: 6232526Abstract: The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.Type: GrantFiled: May 14, 1999Date of Patent: May 15, 2001Assignee: Dekalb Genetics Corp.Inventors: David McElroy, Alan L. Kriz, Emil M. Orozco, Jr., Matt Griffor
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Patent number: 6177613Abstract: The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The composition is a novel nucleic acid sequence for a seed-preferred promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the seed-preferred promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.Type: GrantFiled: January 8, 1999Date of Patent: January 23, 2001Assignee: Pioneer Hi-Bred International, Inc.Inventors: Sean J. Coughlan, Ronnie J. Winfrey, Jr.
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Patent number: 6127601Abstract: Coat protein genes of cucumber mosaic virus strains V27, V33, V34 and A35 (CMV V27, CMV V33, CMV V34, and CMV A35 respectively) are provided.Type: GrantFiled: September 29, 1997Date of Patent: October 3, 2000Assignee: Seminis Vegetable Seeds, Inc.Inventors: Maury L. Boeshore, Russell J. McMaster, David M. Tricoli, John F. Reynolds, Kim J. Carney
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Patent number: 6114601Abstract: The present invention relates to a DNA which encodes a polypeptide having flavonoid-3',5'-hydroxylase activity, a recombinant DNA containing said DNA, and a plant having a pigment pattern which the plant does not originally have and which is acquired by transformation with said recombinant DNA.Type: GrantFiled: February 23, 1996Date of Patent: September 5, 2000Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Yasuhiro Kikuchi, Shigeto Kiyokawa, Yukihisa Shimada, Masaya Ohbayashi, Ritsuko Shimada, Yasushi Okinaka
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Patent number: 6100092Abstract: Products and methods for amplifying target nucleic acids using cells derived from plants are disclosed. The products include nucleic acids containing a plant active Amplification Promoting Sequence (APS) and the methods exploit these products in amplifying target nucleic acids. Also disclosed are methods for amplifying target nucleic acids that express an encoded product, and the recovery of that expression product. The methods of the invention minimize operator intervention and exploit solar energy and the minimal nutrient needs of photoautotrophic organisms to provide inexpensive and indefinitely sustainable methods for producing a variety of amplified target nucleic acids and encoded products such as polypeptides.Type: GrantFiled: June 15, 1998Date of Patent: August 8, 2000Assignee: Board of Trustees, Rutgers The State University of New JerseyInventors: Mykola Borysyuk, Lyudmyla Borysyuk, Ilya Raskin
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Patent number: 6100446Abstract: The disclosure relates to the production and the use, by genetic engineering, of plasmids and bacterial strains containing, on a short, precisely characterizable DNA segment, the gene tfdA or a gene almost identical to tfdA.The novel plasmids and microorganisms are suitable for the production of 2,4-D (2,4-dichlorophenoxyacetic acid)-monooxygenase, and as starting materials for the transfer, by genetic engineering of the 2,4-D-degrading property of this enzyme to various organisms.Type: GrantFiled: June 7, 1995Date of Patent: August 8, 2000Assignee: Hoechst Schering AgrEvo GmbHInventors: Wolfgang R. Streber, Kenneth N. Timmis, Meinhart H. Zenk
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Patent number: 6096547Abstract: Constructs are provided for expression of physiologically active mammalian proteins in plant cells, either in culture or under cultivation. The constructs provide a promoter functional in a plant host, a structural gene coding for mammalian protein and a terminator functional in a plant host. The construct is introduced into a plant cell to become integrated into the plant genome for expression in the plant cells or plants. The plant cells may be harvested and the mammalian protein isolated in physiologically active form.Type: GrantFiled: July 31, 1998Date of Patent: August 1, 2000Assignee: Calgene, LLCInventors: Robert M. Goodman, Vic C. Knauf, Catherine M. Houck, Luca Comai
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Patent number: 6093554Abstract: The invention relates to genetic manipulations of eukaryotic organisms, with recombinant DNA comprising RNA virus derived sequences for protecting such organisms against RNA viruses or enabling inducible or tissue-specific production of foreign proteins/peptides or RNAs. One embodiment of the recombinant DNA according to the invention comprises recombinant DNA, comprising two, 12-250 base pair long, inverted repeat nucleotide sequences with therebetween at least one nucleotide sequence which is derived from RNA virus which for its replication is dependent upon a viral RNA/RNA polymerase, said RNA virus derived sequence comprising at least cis elements for replication but no gene that codes for viral RNA/RNA polymerase and no gene that codes for viral coat protein. The invention also relates to eukaryotic or prokaryotic cells or organisms which incorporate the recombinant DNA according to the invention.Type: GrantFiled: January 21, 1999Date of Patent: July 25, 2000Assignees: Aveve N.V., Clovis MattonInventors: Eddie Van Haute, Paul Ameloot, Jean De Lafonteyne, Walter Fiers
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Patent number: 6080913Abstract: The present invention provides methods for genetically modifying plants to increase the levels of essential amino acids in seed. The present methods involve a combination of:a) providing an increased reservoir or source of a target amino acid population in vegetative tissue; withb) a complementary protein sink.Type: GrantFiled: September 25, 1996Date of Patent: June 27, 2000Assignee: Pioneer Hi-Bred International, Inc.Inventors: Mitchell C. Tarczynski, Paul E. Staswick
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Patent number: 6046382Abstract: A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which neutralizes the activity of another product which disrupts the metabolism, functioning and/or development selectively of the plant's flower cells, particularly reproductive organ cells, or seed cells or embryo cells. The foreign DNA sequence also optionally encodes a marker.Type: GrantFiled: February 10, 1998Date of Patent: April 4, 2000Assignee: Plant Genetic Systems, N.V.Inventors: Celestina Mariani, Jan Leemans, Willy De Greef