Abstract: A method for clonal propagation by somatic polyembryogenesis. The method allows for clonal propagation of embryonal suspensor mass resulting in true-to-type suspensor development of the conifer embryo leading to development of plantlets and plants.

Abstract: Processes for the extraction of taxol and derivatives thereof from roots of plants of the genus Taxus are described.

Abstract: This invention relates to a method for regeneration of coniferous plants. In particular, this invention relates to an improved method for producing and developing somatic embroyos for somatic embryogenesis processes for plants of the genus Pinus and Pinus interspecies hybrids by including polyethylene glycol in the development media. This method is well suited for producing clonal planting stock useful for reforestation.

Abstract: This invention relates to a method for regeneration of coniferous plants. In particular, this invention relates to an improved method for developing viable stage 3 embryos from embryogenic cultures for somatic embryogenesis processes for plants of the genus Pinus and Pinus interspecies hybrids. This method is well suited for producing clonal planting stock useful for reforestation.

Abstract: This invention relates to a method for regeneration of coniferous plants. In particular, this invention relates to an improved method for producing and developing somatic embryos for somatic embryogenesis processes for plants of the genus Pinus and Pinus interspecies hybrids by including polyethylene glycol in the development media. This method is well suited for producing clonal planting stock useful for reforestation.

Abstract: The invention relates to a method for regeneration of plants of the genus Pinus by culturing explants of immature zygotic embryos on culture initiation medium containing abscisic acid, nutrients, growth hormones, sugar and a gelling agent to grow embryogenic tissue for cryopreservation. Culturing of the embryogenic tissue is continued on culture maintenance medium, embryo development medium, and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.

Abstract: This invention relates to the production of genetically homogeneous cell lines from coniferous plants. In particular, this invention describes a method for producing isogenic cell lines of embryogenic cultures derived from immature conifer seeds. This method is suited for producing clonal planting stock useful for reforestation.

Abstract: An aqueous medium for plant tissue culture is provided including a nutritively effective concentration of reduced nitrogen, preferably in the form of an amino acid such as arginine employed in an amount of from 1.0 to 5.0 mM, and having not more that 1.5 mM NH.sub.4.sup.+. A phosphorus compound at a concentration of at least 2.5 mM measured as phosphate is also included. A chelating agent such as EDTA, and an iron compound present in molar excess over the concentration of the chelating agent are employed to control divalent ion concentrations. Potassium ions are maintained at a concentration of below 14 mM. The use of media containing ammonium ions at less than 1.5 mM combined with a supply of reduced nitrogen in the form of arginine, with low potassium levels, has proved useful as media for organized growth, particularly for use with coniferous plants.

Abstract: 2-keto-L-gulonic acid is in high yield produced by contacting a microorganism of the genus Pseudogluconobacter, either as it is or after processing, with L-sorbose.