Abstract: Protocols for organogenic regeneration of cotton are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO3. These explants, prior to shoot initiation, may be transformed with exogenous DNA, either through inoculation with an Agorbacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.

Abstract: The invention relates in general to plants, plant cells, methods of making, and methods of using plants and plant cells transformed to contain a DNA sequence encoding an AMPA-N-acetyltransferase, and to plants and plant cells exhibiting resistance to AMPA in an amount which inhibits the growth of a plant or plant cell lacking a sequence encoding an AMPA-N-acetyltransferase.

Abstract: The present invention relates to the isolation of two DNA promoters from a coffee plant. The isolated promoters, one inducible and one constitutive, are capable of inducing the expression of a second DNA operably linked to the promoter. The present invention also relates to host cells, expression systems and transgenic plants containing the promoters of the invention.

Abstract: Nucleic acid molecules that encode a plant promoter involved in photoperiodism and circadian rhythms are disclosed. These molecules may be introduced into plants in order to alter the photoperiodic and/or circadian clock-based gene expression of the plants.

Abstract: Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and tansgenic plants expressing the modified endotoxin are also aspects of the invention.

Abstract: The present invention relates to a tissue culture process for producing a large number of viable cotton plants in vitro from a specified tissue of cotton plant. The invention provides genotype independent, direct, multiple shoot proliferation and opens up new possibilities for micropropagation, selection of mutants and for producing genetically improved cotton plants by modern methods of agrobiotechnology and genetic engineering. The protocol provides an important step in the success of cotton improvement programmed, utilizing tissue culture technology.

Abstract: The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

Abstract: The present invention provides methods for testing gene expression in a cotton fiber cells. The methods comprise contacting the cell with Agrobacterium sp., comprising a recombinant T-DNA vector, which includes a plant promoter operably linked to a gene of interest; and detecting the product of the polynucleotide of interest, thereby testing for expression of the polynucleotide of interest.

Abstract: Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

Abstract: The present invention relates to a method for rapidly introducing genes into germplasm which involves the use of crossing, backcrossing, intense selection and agronomic trait selection. More specifically, the method comprises the steps of: (a) crossing two parents of two different species, one of the parents (the donor parent) contains the gene of interest; (b) backcrossing the resultant progeny with the parent not containing the gene of interest (recurrent parent) for two to four backcrosses; (c) performing intense selection on a segregating generation of the progeny from the backcrossing step; and (d) selecting final germplasm on the basis of desired agronomic traits. The present invention further relates to two new and distinctive high yielding Pima Bt (Bacillus thuringiensis) Bollgard.RTM. cotton cultivars, designated 926 B Pima and 930 B Pima, which have been prepared in accordance with the method of the present invention.

Abstract: The present invention relates to a method for rapidly introducing genes into germplasm which involves the use of crossing, backcrossing, intense selection and agronomic trait selection. More specifically, the method comprises the steps of: (a) crossing two parents of two different species, one of the parents (the donor parent) contains the gene of interest; (b) backcrossing the resultant progeny with the parent not containing the gene of interest (recurrent parent) for two to four backcrosses; (c) performing intense selection on a segregating generation of the progeny from the backcrossing step; and (d) selecting final germplasm on the basis of desired agronomic traits. The present invention further relates to two new and distinctive high yielding Pima Bt (Bacillus thuringiensis) Bollgard.RTM. cotton cultivars, designated 926 B Pima and 930 B Pima, which have been prepared in accordance with the method of the present invention.

Abstract: A novel cotton cultivar, designated DP 2379, is disclosed. The invention relates to the seeds of cotton cultivar DP 2379, to the plants of cotton DP 2379 and to methods for producing a cotton plant by crossing the cultivar DP 2379 with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar DP 2379 with another cotton cultivar.

Abstract: A method for producing somaclonal variant cotton plant. The method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

Abstract: A somaclonal variant cotton plant. The somaclonal cotton plant is produced by a method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

Abstract: A method is provided for regenerating cotton plants from explant tissue. The improved method allows the generation of embryogenic callus from a cotton tissue explant which is not cultivated on cotton initiation media having exogenous plant hormones. The method can be utilized in the transformation of cotton plants, by cutting cotton tissue to form an explant, co-cultivating the cotton explant tissue with Agrobacterium comprising a DNA sequence of interest, and culturing the co-cultivated explant on cotton initiation media comprising a selective agent but having no exogenous plant hormones. In this fashion transformed cells are induced to produce embryogenic callus on hormone-free selective media.

Abstract: The present invention is a method for rendering cotton fiber cells that are post-anthesis and pre-harvest available for analysis of their physical properties. The method includes the steps of hydrolyzing cotton fiber cells and separating cotton fiber cells from cotton ovules thereby rendering the cells available for analysis. The analysis of the fiber cells is through any suitable means, e.g., visual inspection. Visual inspection of the cells can be accomplished by placing the cells under an instrument for detection, such as microscope or other means.

Abstract: A computer graphics apparatus includes a walk-through function to simulate with a computer the concept of walking through a structure such as a building. Display processing time and calculation processing time for one frame of a computer graphic image are improved by an input unit which receives a viewline moving instruction to move a viewline of a computer image. A viewline control unit determines positions and directions of the viewline while a storage unit stores computer graphics data including three-dimensional data of objects, walk-through attribute data and viewline data. An image generation unit then generates images including animated images based on the position of the viewline and the computer graphics data. The resultant images are then displayed on an image display unit.

Abstract: A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.