Abstract: A one-step process for the lysis of microalgae cell walls and separation of the cellular lipids for use in biofuel production by utilizing a hydrophilic ionic liquid, 1-butyl-3-methylimidazolium. The hydrophilic ionic liquid both lyses the microalgae cell walls and forms two immiscible layers, one of which consists of the lipid contents of the lysed cells. After mixture of the hydrophilic ionic liquid with a suspension of microalgae cells, gravity causes a hydrophobic lipid phase to move to a top phase where it is removed from the mixture and purified. The hydrophilic ionic liquid is recycled to lyse new microalgae suspensions.

Abstract: This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme sour

Abstract: The process according to the invention for the microbial production of a specific product and methane comprises the following steps: a) the production in a bioreactor (2, 10) of a specific product from a specific substrate using a specific organism, the producer, wherein during the production of this product metabolites such as e.g. low-molecular alcohols, aldehydes and acids, hydrogen and CO2, which may inhibit the production of the product, are obtained; and b) the production of methane by means of a methanogen, wherein in the production of the methane at least one metabolite is decomposed and by this means removed from the bioreactor (2, 10) and its energy is made usable.

Abstract: The cultivation of heterotrophic and phototrophic micro-organisms is integrated for the production of bio-oil for biofuels, wherein the overall algal suspension produced is first thickened, with recirculation of the excess water to cultivation containers, and then thermally treated at a high temperature. After cooling, a bio-oil phase is recovered together with a suspension rich in soluble carbohydrates and proteins which forms a nutritional/energy source for heterotrophic micro-organisms.

Abstract: The present invention relates to a composition for bath additives characterized in that the composition is obtained by a process comprising subjecting herbal materials comprising 10˜25% by weight of Zizania latifolia, 10˜20% by weight of Artemisia capillaries, 5˜15% by weight of Houttuynia cordata, 1˜20% by weight of Saururus chinensis, 1˜15% by weight of Leonurus sibiricus, 1˜15% by weight of Angelica gigas, 1˜15% by weight of Coix lachryma-jobi, 1˜10% by weight of Plantago asiatica and 1˜10% by weight of Rhododendron brachycarpum, based on the total dry weight of the above herb materials, to liquid phase fermentation with Bacillus subtilis which is anaerobic, to obtain a culture, separating sludge from the resulting culture, drying, sterilizing and pulverizing the culture to give a pulverized sludge, and a process for preparing the same.

Abstract: Fatty acid alcohol esters that may be used as biodiesel are synthesized from microbially produced alcohol and fatty acids using an in situ process. The process utilizes a catalyst capable of esterifying the alcohol and fatty acid products of microorganisms, which are produced from renewable resources.

Abstract: The present invention, in some embodiments thereof, relates to a photocatalytic method of generating hydrogen gas in algae, and, more particularly, but not exclusively, to algal-bacterial co-culture for enhancing the kinetics and improving the yield of algal hydrogen photoproduction.

Abstract: Disclosed are novel microbial compositions and biodegradation processes to treat marine animal or marine animal by-products to produce solid, liquid and lipid fractions that contain useful compounds.

Abstract: A process for integrated utilization of the energy and material contents of hydrolysates and solids obtained in the enzymatic hydrolysis of renewable raw materials, in which the resulting hydrolysis solution is used as a carbon source in fermentations and the unhydrolysed solids are sent to biogas production.

Abstract: One of the most important technical barriers for algal biofuel is the substantial harvesting and extracting cost. The present invention includes a system and method for the pelletization of single cell microalgae through co-cultivation with filamentous fungi to enable the low cost separation of microalgae from liquid medium and to significantly increase the biomass and lipid yield. The approach represents a simple and straightforward method to significantly reduce the cost for algae-based biofuel production and enable the economically viable algae biofuel production.

Abstract: Bacterial compositions effective for inhibiting fungal diseases of potatoes and/or potato sprouting are produced by co-culture of two or more of Pseudomonas fluorescens (NRRL B-21133), Pseudomonas fluorescens biovar (NRRL B-21053), Pseudomonas fluorescens (NRRL B-21102) and Enterobacter cloacae (NRRL B-21050). Compositions produced by co-culture of these bacteria together in the same culture medium are significantly more effective for inhibiting fungi-induced diseases of potatoes and/or inhibiting sprouting of potatoes, than blends or mixtures of the same bacteria cultured separately.

Abstract: The present invention provides a method for generating methane from a carbonaceous fuel source with simultaneous sequestration of carbon dioxide, the method comprising anaerobically incubating a particulate alkaline earth metal salt in contact with a particulate and/or dissolved carbonaceous feedstock in a neutral or alkaline aqueous culture medium containing a culture of methanogenic bacteria consortia and collecting methane generated therefrom. At least a portion of carbon dioxide produced during the incubation reacts with the alkaline earth metal salt to form an alkaline earth metal carbonate, thereby sequestering the carbon dioxide.

Abstract: The present invention relates to an EM-lacquer water solution mixed with a natural component, a method for preparing a fermented anti-oxidant material using the same, and a method for processing the material. Particularly, the EM-lacquer water solution mixed with a natural component is prepared by mixing an EM-lacquer solution, which is a mixture of a crude solution of effective micro-organisms (EM) 20˜35 weight percent and a lacquer water solution 65˜80 weight percent, and at least one natural component selected from a group comprising of organic brown sugar, bay salt, mandarin orange oil, Japanese apricot concentrate, Opuntia humifusa powder, dried orange peel powder, green tea powder, sea tangle powder, rice bran, camellia oil and bean powder, on the basis of the EM-lacquer water solution 20 L. Therefore, it can be used for daily life, industry, environmental purification and the like because it is made of non-toxic materials.

Abstract: The present invention provides a process for the preparation of ethanol from starch. Specifically, the present invention provides a process for the preparation of ethanol from starch such as tapioca, potato, sweet sorghum, rice by liquification and saacharification of starch and subsequent fermentation of mono saccherides to ethanol in presence of thermophilic micro-organisms.

Abstract: A method for stimulating enhanced lipid accumulation by algae includes growing algae in a bioreactor medium including nutrients. The algae have an average lipid content that averages a first % of total cell biomass. A stress inducing environmental condition is initiated that keeps the algae alive, stops cell reproduction, and induces the algae to accumulate additional lipids resulting in a second average lipid content that is at least 50% more than the first %. The method can include measuring a lipid concentration of the algae while under the stress inducing environmental condition, harvesting lipids from more than 50% but not all of the algae when the lipid concentration is above a predetermined lipid limit, adding fresh medium to the bioreactor medium having algae not involved in the harvesting therein, and repeating the method, wherein the algae not involved in harvesting serves as a source for new algae growth.

Abstract: A process for the production of bio-oil from solid urban waste, comprising the following steps: a) subjecting said solid urban waste to liquefaction, obtaining a mixture including an oily phase consisting of bio-oil, a solid phase and an aqueous phase; b) subjecting the aqueous phase obtained in the liquefaction step a) to fermentation, obtaining a fermented biomass; c) feeding the fermented biomass obtained in the fermentation step b) to the liquefaction step a). The bio-oil (or bio-crude) thus obtained can be advantageously used in the production of biofuels which can be used as such or mixed with other motor vehicle fuels. Alternatively, this bio-oil (or bio-crude) can be used as such (biocombustible) or mixed with fossil combustibles (combustible oil, coal, etc.) for the generation of electric energy or heat.

Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

Abstract: A method for identifying a biological analyte that is affected by a stressor is disclosed in which two substantially identical biological samples are provided, with a first sample being a control sample and a second sample being an experimental sample. The control sample is grown with a nutrient having an isotope of a first atom, whereas the experimental sample is grown with a nutrient having a second isotope of the first atom. The experimental sample is grown with a stressing agent and regimen. The samples are admixed, and the formed composite is mass spectroscopically assayed for analyte peaks. The ratio of first isotope to second isotope is determined for the peaks, as is a sample median isotopic ratio. The ratio for assayed analyte peaks is compared with the median ratio. An analyte whose isotopic ratio significantly deviates from the median ratio is an analyte affected by the stressing agent.

Abstract: The present invention relates to the enhanced production of metabolites by a process whereby a carbon source is oxidized with a fermentative microbe in a compartment having a portal. An electron acceptor is added to the compartment to assist the microbe in the removal of excess electrons. The electron acceptor accepts electrons from the microbe after oxidation of the carbon source. Other transfers of electrons can take place to enhance the production of the metabolite, such as acids, biofuels or brewed beverages.

Abstract: The present invention relates to the antagonistic bacteria that are used to prevent and eliminate tobacco bacterial wilt and their microbial organic fertilizer, which belong to the technology for agricultural intensive production. The present invention separates two antagonistic bacteria (Brevibacillus brevis NJL-25 and Bacillus cereus NJL-14) that have remarkable antagonistic action against the pathogens of tobacco bacterial wilt. Microbial organic fertilizer is produced from these antagonistic bacteria and organic compost. Wherein, the content of each of NJL-25 and NJL-14 is above 1×1010 cfu/g, the content of total nitrogen is 4˜5% (above 90% of the nitrogen is organic nitrogen), the content of total nitrogen-phosphorus-kalium nutrient is 6˜10% and the content of organic matter is 30˜35%. As indicated by experiments, after the microbial organic fertilizer is applied into soil, it will enable rapid multiplication of the antagonistic bacteria into a dominant microflora in the soil and achieve more than 97.

Abstract: Methods and systems for purifying bio-organic compounds are described. In certain embodiments, the methods comprise the steps of (a) providing a composition or an emulsion comprising a surfactant, host cells, an aqueous medium and a bio-organic compound produced by the host cells, wherein the solubility of the surfactant in the aqueous medium decreases with increasing temperature and wherein the temperature of the composition or emulsion is at least about 1° C. below a phase inversion temperature of the composition or emulsion; (b) raising the temperature of the composition or emulsion to at least about 1° C. above the phase inversion temperature; and (c) performing a liquid/liquid separation of the composition to provide a crude bio-organic composition or emulsion.

Abstract: A system for the efficient conversion of plant biomass to methane is provided, where the conversion includes use of a thermophilic methanogenic consortium containing a cellulolytic thermophile, an acetate-oxidizing thermophile and a thermophilic methanogen, the combination of which hydrolyzes hexoses and pentoses, oxidizes acetate and provides a hydrogen sink, to convert plant biomass to the theoretical limit of bioenergy.

Abstract: This invention provides a method of producing cyathin A3 in a culture of Cyathus helenae. It employs added bacteria to stimulate production of cyathin A3.

Abstract: An isolated microbial consortium is described that includes a first microbial consortium having Thermacetogenium phaeum to metabolize a complex hydrocarbon substrate into metabolic products comprising an acetate compound. The consortium also includes a second microbial consortium having a methanogen to convert the acetate compound into a final product that includes methane. Also, a method of increasing production of materials with enhanced hydrogen content. The method includes isolating Thermacetogenium phaeum from geologic formation water, culturing the isolated Thermacetogenium phaeum to increase the Thermacetogenium phaeum population, and introducing a consortium of the cultured Thermacetogenium phaeum, which may include spores of Thermacetogenium phaeum, into a hydrocarbon formation containing a complex hydrocarbon substrate.

Abstract: The present invention relates to a method of producing fatty acids, by inoculating a mixture of at least one of cellulose, hemicellulose, and lignin with a microorganism strain and an algae strain, and growing said inoculated strains under successive aerobic-heterotrophic and either anaerobic-phototrophic or anaerobic-heterotrophic conditions creating symbiosis between the strains. Under a first aerobic-heterotrophic condition, the microorganism strain produces extracellulases that hydrolyze cellulose, hemicellulose and lignin, to produce sugars, such as glucose, cellobiose, xylose, mannose, galactose, rhamnose, arabinose or other hemicellulose sugars that are metabolized by the algae strain which also metabolizes acetic acid, glucose and hemicellulose from pretreatment.

Abstract: A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products.

Abstract: A method of detection of cells, microorganisms, or molecules by the use of various combinations of fluorogens and a chromogens which yield fluorophores and chromophores when cleaved by specific enzymes and which can be viewed by UV and visible light. Included is the method of application of a family of compounds producing both insoluble fluorophores and chromophores identified as dual enzyme substrates.

Abstract: The present invention provides a microbial hydrogen-producing process, comprising: providing at least one Clostridium microbe and at least one Bacillus microbe; and co-culturing said at least one Clostridium microbe and said at least one Bacillus microbe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least one Clostridium microbe and at least one Bacillus microbe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.

Abstract: A method for controlling Dipteran larvae or a method for inhibiting the development of larvicidal resistance, controlling resistant populations and reducing resistance levels in Diptera by introducing a larvicidally-effective amount of a combination of a strain of Bacillus thuringiensis subspecies israelensis and a strain of Bacillus sphaericus into an environment containing Dipteran larvae; and a composition of the combination are disclosed. Preferably both strains are non-genetically modified.

Abstract: The invention relates to systems and methods for production of compounds by yeast and other organisms. In one approach yeast engineered for production of a compound of commercial value is cultured together with a cellulosic bacteria, and the yeast uses a metabolic product produced by the bacteria as a carbon source. Methyl halides are an example of compounds that may be produced by this process. The invention also relates to production of organic compounds using genetically engineered organisms expressing a S-adenosylmethionine (SAM)-dependent methyl halide transferase. In one approach the organism, halides and a carbon source are incubated in a cultivation medium under conditions in which methyl halide is produced. The methyl halide may be collected and converted into non-halogenated organic molecules.

Abstract: The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g/L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when

Abstract: Disclosed are novel microbial compositions and biodegradation processes to treat marine animal or marine animal by-products to produce solid, liquid and lipid fractions that contain useful compounds.

Abstract: The present invention is directed to a process for producing CMP-N-acetylneuraminic acid (CMP-NeuAc), comprising adding to the cultured E. coli cells which has been transformed with both the DNA encoding N-acetylglucosamine-6-phosphate 2-epimerase (GlcNAc-6P 2-epimerase) and the DNA encoding N-acetylneuraminic acid synthase (NeuAc synthase) and exhibit activities of N-acetylglucosamine-6-phosphate 2-epimerase and N-acetylneuraminic acid synthase, a phosphate buffer containing baker's yeast cells, CMP, N-acetylglucosamine (GlcNAc), magnesium, xylene, glucose, and CMP-N-acetylneuraminic acid synthase (CMP-NeuAc synthase) to provide a reaction mixture, and allowing the reaction to proceed and produce CMP-N-acetylneuraminic acid (CMP-NeuAc), and wherein the process does not require adding ATP.

Abstract: The invention concerns a mixture of bacteria. Said mixture is a non starter culture which is free from metabolites and comprises at least one first bacterium selected from the species <i>Propionibacterium jensenii</i> and at least one second bacterium selected from the genus <i>Lactobacillus</i>. Furthermore, food, feeding stuff and medicaments comprising such a mixture, a method for manufacturing and storing such goods and the use of the mixture to inhibit fungi and bacteria are provided.

Abstract: In a method of producing biogas by anaerobic digestion of organic matter, organic matter is dried to a dry solids content of at least 50% by weight TS and pelletized and then mixed with a liquid to form a slurry. The slurry is contacted with biogas-producing bacteria for digestion under anaerobic conditions in a reactor (102) while producing biogas.

Abstract: The invention relates to a method for increasing biogas production of thermophilic anaerobe systems. More closely, a biomass containing a consortium comprising polymer-degrading, acetogenic and methanogenic microorganisms is inoculated with a microorganism culture before the start of or during biogas production. Said microorganism is a thermophilic, acetogenic, hydrogen producing bacterium. As an example, Caldicellulosiruptor saccharolyticus is applied. According to the invention production if methane containing biogas is significantly increased. The invention is useful for intensifying biogas production and thereby increasing its feasibility.

Abstract: An isolated microbial consortium is described that includes a first microbial consortium having Thermacetogenium phaeum to metabolize a complex hydrocarbon substrate into metabolic products comprising an acetate compound. The consortium also includes a second microbial consortium having a methanogen to convert the acetate compound into a final product that includes methane. Also, a method of increasing production of materials with enhanced hydrogen content. The method includes isolating Thermacetogenium phaeum from geologic formation water, culturing the isolated Thermacetogenium phaeum to increase the Thermacetogenium phaeum population, and introducing a consortium of the cultured Thermacetogenium phaeum, which may include spores of Thermacetogenium phaeum, into a hydrocarbon formation containing a complex hydrocarbon substrate.

Abstract: The present invention refers to a process to produce biodiesel and/or fuel oil from microbial oilseed and/or algal biomass and/or from sugar cane residues and derivatives. The products according to the present invention are appropriate for direct use in motors and to generate energy or steam. The integrated process of the present invention comprises the use, as raw materials, of microbial oil-producing biomass obtained from sugar cane residues and derivatives, which is integrated with algal biomass and/or glycerol and are processed by steps of production of oil-producing microbial biomass from filamentous fungi and/or yeasts, steps of simultaneous production of algal biomass by fully using residues, CO2 and residual broth of said production of microbial biomass, as well as steps of extraction and transesterification of lipids contained in the biomass, with reuse of the residual glycerol thus produced.

Abstract: Methods for increasing methane emitted from organic waste and for collecting methane and other gaseous bi-products. A gas collection unit collects gas in a first separation tank, wherein the gas is separated into methane and other components. The methane is collected, and other components are diverted. A second separation tank receives diverted components combined with an acidic solution and further separates the components into carbon dioxide and other compounds. The carbon dioxide is collected for reuse in a variety of applications.

Abstract: The present invention relates to a single flat-based well suitable for use in a viral diagnostic method. More particularly, the well has a planar or flat base, as opposed to a curved base. The invention also relates to a viral diagnostic method that employs such single wells. In an embodiment of this method a specially developed tissue culture medium supplemented with hormones and enzymes is employed.

Abstract: The present invention identifies that the expression of Activation Induced Deaminase (AID) or its homologues in cells confers a mutator phenotype and thus provides a method for generating diversity in a gene or gene product as well as cell lines capable of generating diversity in defined gene products. The invention also provides methods of modulating a mutator phenotype by modulating AID expression or activity.

Abstract: A method for identifying a biological analyte that is affected by a stressor is disclosed in which two substantially identical biological samples are provided, with a first sample being a control sample and a second sample being an experimental sample. The control sample is grown with a nutrient having an isotope of a first atom, whereas the experimental sample is grown with a nutrient having a second isotope of the first atom. The experimental sample is grown with a stressing agent and regimen. The samples are admixed, and the formed composite is mass spectroscopically assayed for analyte peaks. The ratio of first isotope to second isotope is determined for the peaks, as is a sample median isotopic ratio. The ratio for assayed analyte peaks is compared with the median ratio. An analyte whose isotopic ratio significantly deviates from the median ratio is an analyte affected by the stressing agent.

Abstract: Alternative and improved approaches to the heterologous expression of the proteins of Neisseria meningitidis and Neisseria gonorrhoeae. These approaches typically affect the level of expression, the ease of purification, the cellular localization, and/or the immunological properties of the expressed protein.

Abstract: The present invention relates to the identification of gene sequences and proteins involved in vaccinia virus dominant T cell epitopes. Two vaccinia virus CD8+ T cell epitopes restricted by the most common human MHC class I allele, HLA-A0201 have been identified. Both epitopes are highly conserved in vaccinia and variola viruses. The induction of the T cell responses following primary vaccination is demonstrated by the kinetics of epitope specific CD8+ T cells in 3 HLA-A0201 individuals. This information will be useful for the design and analyses of the immunogenicity of experimental vaccinia vaccines, and for basic studies of human T cell memory.

Abstract: The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

Abstract: For the purpose of forming a biofilm having amount and strength sufficient for the immobilization of a cell mass, the present invention provides a method for producing a biofilm, characterized in that it comprises co-culturing lactic acid bacteria belonging to Lactobacillus plantarum with yeast.

Abstract: Antimicrobial agents that can serve as replacements to conventional pharmaceutical antibiotics are disclosed. The antimicrobial agents comprise conjugatively transmissible plasmids that kill targeted pathogenic bacteria, but are not harmful to donor bacteria. Two types of lethal transmissible plasmids are disclosed. One type kills recipient bacteria by unchecked (“runaway”) replication in the recipient cells and is prevented from occuring in donor cells. Another type kills recipient bacteria by expressing a gene that produces a product detrimental or lethal to recipient bacterial cells, that gene being prevented from expression in donor cells.

Abstract: The invention discloses a method of maintaining hepatitis C virus (HCV) growing indefinitely in cell culture. The method includes providing a culture of cells susceptible to infection by HCV; introducing to the cell culture an inoculum containing an infective dose of HCV; contacting the inoculated cell culture with a growth medium containing an excess of uridine and cytidine; and changing spent growth medium with fresh growth medium containing the excess of uridine and cytidine on a predetermined schedule.

Abstract: A process for producing soap is provided, which includes effective microorganisms (EM) and EM-X ceramic powder, which are added to enhance the degree of saponification of fat, strengthen the cleaning power, and to provide a detergent that is capable of proliferating effective microorganisms in sewage water after washing and cleaning the sewage water, and which exhibits an effect as a water purification material after washing. Effective microorganisms (EM) including mainly facultative anaerobic lactic acid bacteria, yeast and photosynthetic bacteria and EM-X ceramic powder are added in the process of producing the soap, thereby, the treated material obtained according to the present invention exhibits an environmental purification effect as a substrate of benign microorganisms or a microorganism material.