Fusion Of Cells Patents (Class 435/449)
  • Patent number: 9914766
    Abstract: The present invention relates to a method for generating a plurality of different IgY antibodies, by immunizing each of a plurality of avian organisms of the same or different species with a composition comprising a plurality of different antigens, thereby generating a plurality of different IgY antibodies.
    Type: Grant
    Filed: September 23, 2014
    Date of Patent: March 13, 2018
    Assignee: Carviar ApS
    Inventor: Janus Beierholm Larsen
  • Patent number: 9896699
    Abstract: The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: February 20, 2018
    Assignee: New York Stem Cell Foundation, Inc.
    Inventor: Dietrich M. Egli
  • Patent number: 9045761
    Abstract: Embodiments of the present invention relates to the incorporation and use of a regulated genetic suicide mechanism for use in the improved purification of biologics, including adjunct use in various eubacterial minicell production and purification methodologies. Described herein are high-yield eubacterial minicell-producing strains with genetic modifications that comprise a regulated genetic suicide mechanism that irreparably destroys the parent cell chromosome such that live parental cells in a culture can be functionally eliminated at any time during the course of a minicell production and purification run. Embodiments of the present invention also describe methods useful in the elimination of live parental cells during the production of other cell-based biologics.
    Type: Grant
    Filed: December 11, 2012
    Date of Patent: June 2, 2015
    Assignee: Vaxiion Therapeutics, LLC
    Inventors: Matthew J Giacalone, Stanley Maloy, Shingo Tsuji
  • Publication number: 20150112125
    Abstract: A method of sorting sperm cells and a method of producing at least one sexed embryo. The method of sorting sperm cells includes the steps of establishing a sheath fluid environment including a citrate for stained sperm cells, establishing a stream including stained sperm cells in said sheath fluid environment, sensing a property of the stained sperm cells, and discriminating between stained sperm cells for a desired sex characteristic. The method of producing at least one sexed embryo includes the steps for sorting sperm cells in addition to fertilizing at least one egg with the sexed sperm to form at least one sexed embryo.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 23, 2015
    Applicant: XY, LLC
    Inventors: George E. Seidel, Lisa A. Herickhoff, John L. Schenk
  • Publication number: 20150082471
    Abstract: A sustained culture of isolated avian gonocytes is provided, as well as a method of making and using the same. A chimeric avian containing an isolated gonocyte and a transgenic avian produced using the chimeric avian are also provided. The cell and method may be employed to make, among other things, transgenic avian that produce a heterologous protein, e.g., a therapeutic protein.
    Type: Application
    Filed: September 16, 2014
    Publication date: March 19, 2015
    Inventors: Marie-Cecile van de Lavoir, Robert Etches
  • Patent number: 8945878
    Abstract: The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies.
    Type: Grant
    Filed: September 6, 2013
    Date of Patent: February 3, 2015
    Assignee: OncoMed Pharmaceuticals, Inc.
    Inventors: Austin L. Gurney, Alexandra L. L. Lazetic, Christopher J. Bond
  • Publication number: 20150024496
    Abstract: Disclosed herein include embodiments related to delivery of molecular molecules by a sperm cell to an egg cell for expression, including transient expression, in a fertilized progeny. Further embodiments relate to computerized systems for assisting in the disclosed methods.
    Type: Application
    Filed: July 19, 2013
    Publication date: January 22, 2015
    Inventors: Roderick A. Hyde, Tony S. Pan, Lowell L. Wood, JR.
  • Patent number: 8900870
    Abstract: A method of forming a hybridoma cell includes acoustically focusing antibody producing cells in a first channel having a first acoustic field so as to produce a single file line of antibody producing cells; acoustically focusing immortal cells in a second channel having a second acoustic field so as to produce a single file line of immortal cells; flowing the acoustically focused single file lines of antibody producing cells and immortal cells to a third channel having a third acoustic field configured to bring at least one antibody cell and at least one immortal cell into close enough proximity to permit them to fuse; and fusing the antibody producing cell and the immortal cell together by at least one of a chemical and an electrical means to form at least one hybridoma cell.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: December 2, 2014
    Assignee: Life Technologies Corporation
    Inventors: Michael D. Ward, Gregory Kaduchak
  • Patent number: 8901099
    Abstract: It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: December 2, 2014
    Assignee: Sekisui Chemical Co., Ltd.
    Inventors: Joe Chiba, Jun-ichi Hata, Naoki Nishiguchi, Masahiro Furutani
  • Publication number: 20140308257
    Abstract: The present invention provides modified oocytes having a nuclear genome derived from a first oocyte and cytoplasm derived from a second oocyte from a different subject, and methods for making and using such modified oocytes. The methods and compositions of the present invention can be useful in a variety of settings including, but not limited to, in in vitro fertilization (“IVF”) procedures.
    Type: Application
    Filed: December 5, 2013
    Publication date: October 16, 2014
    Applicant: New York Stem Cell Foundation
    Inventor: Dietrich M. Egli
  • Patent number: 8859233
    Abstract: The present invention relates to a method for generating a plurality of different IgY antibodies, by immunizing each of a plurality of avian organisms of the same or different species with a composition comprising a plurality of different antigens, thereby generating a plurality of different IgY antibodies.
    Type: Grant
    Filed: May 1, 2007
    Date of Patent: October 14, 2014
    Assignee: Carviar APS
    Inventor: Janus Beierholm Larsen
  • Publication number: 20140248700
    Abstract: An improved method of producing differentiated progenitor cells comprising obtaining inner cell mass cells from a blastocyst and inducing differentiation of the inner cell mass cells to produce differentiated progenitor cells. The differentiated progenitor cells may be transfected such that there is an addition, deletion or alteration of a desired gene. The differentiated progenitor cells are useful in cell therapy and as a I source of cells for the production of tissues and organs for transplantation. Also provided is a method of producing a lineage-defective human embryonic stem cell.
    Type: Application
    Filed: May 7, 2014
    Publication date: September 4, 2014
    Applicant: Advanced Cell Technolgy
    Inventors: Jose Cibelli, Michael West, Robert Lanza
  • Publication number: 20140249364
    Abstract: Ovarian germ-line-competent embryonic stem cells (GLC-ESC) are cultured, either in the presence or absence of a compound having estrogenic activity. The GLC-ESC are either collected prior to specific commitment or are permitted to remain in the culture medium for a time sufficient to develop into oocytes, and the oocytes may be fertilized by adding sperm to the culture medium. The fertilized oocytes may be permitted to develop into embryos, which may be transferred into the uterus of an adult human female or frozen for later use. The invention provides a method for obtaining by in vitro fertilization an embryo that is genetically related to a human female who is not producing oocytes.
    Type: Application
    Filed: October 2, 2013
    Publication date: September 4, 2014
    Applicant: OVASCIENCE, INC.
    Inventors: Antonin BUKOVSKY, Michael R. CAUDLE
  • Publication number: 20140234968
    Abstract: Immunocompatible pluripotent stem cells (pSCs), which include cells compatible with different patient populations or patient-specific cells, find wide application in regenerative medicine therapies. Described herein are immunocompatible pSCs generated using techniques such as parthenogenesis resulting in cells possessing desired haplotypes of reduced zygosity, antigenically compatible with multiple patient populations, or nuclear transfer allowing generation of patient-specific cells. Methods described herein related to parthenogenesis, nuclear transfer, or pSC cell line generation. Also described herein are compositions of immunocompatible pSCs and cell lines generated by the aforementioned techniques.
    Type: Application
    Filed: February 14, 2014
    Publication date: August 21, 2014
    Applicant: Sung Kwang Medical Foundation
    Inventors: Young Gie Chung, Dong Ryul Lee
  • Publication number: 20140199319
    Abstract: Disclosed herein are methods for improving the efficiency of the production of hybridomas, e.g., through enrichment of IgG expressing B cells. Also disclosed are hybridoma compositions produced using these methods as well as methods for producing antibodies with the hybridomas.
    Type: Application
    Filed: December 13, 2013
    Publication date: July 17, 2014
    Applicant: AbbVie, Inc.
    Inventors: Jane SEAGAL, Eve H. BARLOW, Chung-Ming HSIEH, Jeffrey Yen PAN, Shawn M. JENNINGS, Mary R. LEDDY, Archana B. THAKUR
  • Patent number: 8778349
    Abstract: The intrinsically disordered sequences—or “intrinsically disorded sequences” or IDSeq—proteins should be flexible to ensure a controlled interaction between proteins. In the development of the diseases, IDSeq are modified and polymerized. The invention describes the method of preparation of the drugs against cancers, the degenerative diseases and the infectious illness, by the induction of an immune reaction against IDSeq modified in a covalent way (IDSeqC) and polymerized (pIDSeqC), and leading to a new network of protein signaling, named here “misfoldome”, causing the diseases. The invention describes the preparation of vaccines by the use of polymers of IDSeqC. Peptides of the pIDSeqC are prepared in vitro, and introduced into a living organism to induce an immunological response, which eliminates the “misfoldome” and cures the diseases. The method is employed for the preparation of active or passive vaccines.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: July 15, 2014
    Inventor: Halina Malina
  • Patent number: 8735138
    Abstract: The present invention relates to methods for producing Saccharomyces strains that are capable of growth on xylose as a sole carbon source at a desired growth rate (such as at least one generation per 48 hours), strains made by such methods, and Saccharomyces strains that grow at a growth rate of at least one generation per 48 hours using xylose as a sole carbon source for growth made by non-recombinant methods.
    Type: Grant
    Filed: September 16, 2009
    Date of Patent: May 27, 2014
    Assignee: Microbiogen Pty Ltd
    Inventors: Philip John Bell, Paul Victor Attfield
  • Patent number: 8697446
    Abstract: The present invention relates to a cell fusion chamber in which two types of cells having different diameters are fused, the cell fusion chamber including: a cell fusion region in which cell fusion is carried out; a pair of electrodes formed by a conductor and disposed opposite to each other in the cell fusion region; and a partition wall having at least one fine pore; near the fine pore, a cell fusion device including a cell fusion container containing a cell fusion region; a pair of electrodes; a spacer; and an insulator disposed between the spacer and one of the electrodes and having at least one fine pore; and an electronic power supply which applies an alternating voltage and a voltage pulsed direct current to the electrodes, and a cell fusion method using the same.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: April 15, 2014
    Assignee: Tosoh Corporation
    Inventors: Toru Futami, Takahiro Maruyama, Atsushi Morimoto
  • Publication number: 20140057305
    Abstract: Disclosed is an antibody which binds to quetiapine, which can be used to detect quetiapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of quetiapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 27, 2014
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Matthew Garrett Donahue, Yong Gong
  • Publication number: 20140057301
    Abstract: Disclosed is an antibody which binds to risperidone, which can be used to detect risperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of risperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 27, 2014
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Maarten Vliegen, Pieter Rik Haspeslagh
  • Publication number: 20140057297
    Abstract: Disclosed is an antibody which binds to paliperidone, which can be used to detect paliperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of paliperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, risperidone and paliperidone in a single lateral flow assay device.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 27, 2014
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Maarten Vliegen, Pieter Rik Haspeslagh
  • Publication number: 20140057303
    Abstract: Disclosed is an antibody which binds to olanzapine, which can be used to detect olanzapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of olanzapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 27, 2014
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Matthew Garrett Donahue, Yong Gong
  • Publication number: 20140057299
    Abstract: Disclosed is an antibody which binds to aripiprazole, which can be used to detect aripiprazole in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of aripiprazole, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 27, 2014
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Ronghui Lin
  • Patent number: 8642330
    Abstract: Antibody compositions and methods for inhibition of the effects of gonadotropin hormones are provided. Methods for treating cancer and methods for regulating fertility are provided by administration of the antibody compositions to a mammalian subject in need thereof.
    Type: Grant
    Filed: August 8, 2006
    Date of Patent: February 4, 2014
    Assignee: Onconon, LLC
    Inventors: Edwin P. Rock, Vernon C. Stevens, Pierre L. Triozzi
  • Publication number: 20140005366
    Abstract: The present invention provides a method for inducing a cancer specific immune response against MUC1 using an immunogenic glycopeptide. Other aspects of the invention are a pharmaceutical composition comprising the immunogenic glycopeptide and a cancer vaccine comprising the immunogenic glycopeptide. Another aspect is an antibody generated using the immunogenic glycopeptide and the use of said antibody in therapy and diagnosis.
    Type: Application
    Filed: April 16, 2013
    Publication date: January 2, 2014
    Applicants: Cancer Research Technology Limited, Københavns Universitet
    Inventors: Henrik Clausen, Joy Burchell, Ulla Mandel, Anne Louise Sørensen, Mads Agervig Tarp, Joyce Taylor-Papadimitriou
  • Publication number: 20130336939
    Abstract: There are provided methods and compositions useful in cell-cell fusion using Fusion Family (FF) proteins of nematode origin. There are further provided antinematodal methods and compositions, utilizing fusogenic proteins of the nematode Fusion Family.
    Type: Application
    Filed: January 31, 2012
    Publication date: December 19, 2013
    Applicants: UNIVERSITY OF VIRGINIA PATENT FOUNDATION, TECHNION RESEARCH AND DEVELOPEMENT FOUNDATION LTD.
    Inventors: Benjamin Podbilewicz, Ori Avinoam, Judith M. White
  • Publication number: 20130302288
    Abstract: This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
    Type: Application
    Filed: April 18, 2013
    Publication date: November 14, 2013
    Applicant: Advanced Cell Technology, Inc.
    Inventor: Advanced Cell Technology, Inc.
  • Publication number: 20130276159
    Abstract: Provided are a method for preparing a mammalian ovum or embryo in which zona pellucida has been thinned or eliminated, and a method for fertilization using the mammalian ovum prepared by the aforementioned method. The method for thinning or eliminating zona pellucida of a mammalian ovum or embryo involves treating or culturing the mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in a culture medium containing a reducing agent having SH groups (such as reduced glutathione or DTT). The resulting mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in which zona pellucida has been thinned or eliminated is capable of realizing an improved fertilization rate and development rate when used for in vitro fertilization, transplantation of a fertilized ovum, or preparation of an embryo in the early stages of development used in the production of a genetically modified animal.
    Type: Application
    Filed: September 12, 2011
    Publication date: October 17, 2013
    Inventors: Naomi Nakagata, Toru Takeo
  • Patent number: 8551715
    Abstract: The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies.
    Type: Grant
    Filed: February 11, 2011
    Date of Patent: October 8, 2013
    Assignee: OncoMed Pharmaceuticals, Inc.
    Inventors: Austin L. Gurney, Alexandra L. L. Lazetic, Christopher J. Bond
  • Patent number: 8513486
    Abstract: The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and/or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.
    Type: Grant
    Filed: September 8, 2006
    Date of Patent: August 20, 2013
    Assignee: Aarhus Universitet
    Inventors: Yutao Du, Lars Axel Bolund, Gabor Vajta, Peter Michael Kragh
  • Publication number: 20130177987
    Abstract: Disclosed are methods for producing class-switched, affinity-matured antibodies which include enriching an immunized cell population for GL7-positive cells and activating the enriched cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells or to improve the quality of molecularly cloned Ig heavy and light chains.
    Type: Application
    Filed: June 25, 2012
    Publication date: July 11, 2013
    Applicant: NEOCLONE BIOTECHNOLOGY INTERNATIONAL, LLC
    Inventors: Brian Schram, Rachel Kravitz
  • Publication number: 20130150663
    Abstract: Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.
    Type: Application
    Filed: February 11, 2013
    Publication date: June 13, 2013
    Applicant: XY, LLC
    Inventor: XY, LLC
  • Publication number: 20130149784
    Abstract: An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
    Type: Application
    Filed: February 7, 2013
    Publication date: June 13, 2013
    Applicant: XY, LLC
    Inventor: XY, LLC
  • Patent number: 8445229
    Abstract: The invention provides methods for generating high titers of high-affinity antibodies from hybridoma cells produced by fusing myeloma cells with in vitro immunized donor cells. The hybridoma cells or mammalian expression cells with cloned antibody genes from the hybridomas producing the high-affinity antibodies may be mismatch repair defective due to defects of endogenous mismatch repair subunits of through expression of a dominant negative allele of a mismatch repair gene which allows the hybridoma cell to be hypermutable, may be rendered hypermutable by chemical means, or may be naturally mismatch repair deficient. High-affinity antibodies and high titer producer cells producing antibodies may be prepared by the methods of the invention.
    Type: Grant
    Filed: May 25, 2010
    Date of Patent: May 21, 2013
    Assignee: Morphotek, Inc.
    Inventors: Luigi Grasso, Shaohong Liang, Nicholas C. Nicolaides, Philip M. Sass
  • Publication number: 20130086702
    Abstract: The present invention provides manufacturing processes for biological replication of undifferentiated plant and animal cells and tissue in a weightless condition, including those systems used in current stem cell research and development and use of undifferentiated parenchyma in plants. The present invention further provides methods for adapting plants and animals to survive outside their native environments. In particular, undifferentiated cells from plants or animals are replicated under weightless conditions in which cell replication or proliferation is accelerated and sustained. Under such conditions, the undifferentiated cells can be “forced” to express sets of genes useful for survival in particular environmental conditions. In this manner, cells surviving prolonged exposure to specific environmental conditions can be selected for and cultivated to produce an organism adapted to that particular environment in an accelerated manner.
    Type: Application
    Filed: July 14, 2011
    Publication date: April 4, 2013
    Applicant: Zero Gravity, Inc.
    Inventor: John W. Kennedy
  • Publication number: 20130065307
    Abstract: Methods for making human ES cells and human differentiated cells and tissues for transplantation are described, whereby the cells and tissues are created following somatic cell nuclear transfer. The nuclear transfer donor is genetically modified prior to nuclear transfer such that cells of at least one developmental lineage are de-differentiated, i.e., unable to develop, thereby resolving the ethical dilemmas involved in reprogramming somatic cells back to the embryonic stage. The method concomitantly directs differentiation such that the desired cells and tissues may be more readily isolated.
    Type: Application
    Filed: April 16, 2012
    Publication date: March 14, 2013
    Inventor: Jose CIBELLI
  • Publication number: 20130052267
    Abstract: Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods.
    Type: Application
    Filed: July 27, 2012
    Publication date: February 28, 2013
    Inventors: Helen M. Blau, Kostandin Pajcini, Jason Pomerantz
  • Publication number: 20130034906
    Abstract: The invention relates to methods for assisted reproduction technology in mammals. Specifically the invention relates to methods for in vitro mammalian oocyte culture and oocyte maturation, in vitro fertilization, and in vitro embryo development.
    Type: Application
    Filed: January 7, 2011
    Publication date: February 7, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Gary D. Smith, Shuichi Takayama, Charles Bormann, Yunseok Heo
  • Patent number: 8349807
    Abstract: It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.
    Type: Grant
    Filed: October 14, 2005
    Date of Patent: January 8, 2013
    Assignees: Joe Chiba, Sekisui Chemical Co., Ltd.
    Inventors: Joe Chiba, Jun-ichi Hata, Naoki Nishiguchi, Masahiro Furutani
  • Publication number: 20120322156
    Abstract: In accordance with the present invention, a family of membrane fusion protein and polynucleotides encoding the proteins have been identified. The proteins and nucleotides are derived from the family Reoviridae. Two membrane fusion proteins have been isolated from reoviruses isolated from poikilothermic hosts: the p14 protein from reptilian reovirus (RRV) isolated from python, and the p16 protein from aquareovirus (AQV) isolated from salmon. The genes encoding these proteins have been cloned and sequenced. Analysis of the amino acid sequences of these proteins show that both lack the typical fusion peptide motif found in other membrane fusion proteins. Expression of these proteins in cells results in cell-cell fusion.
    Type: Application
    Filed: August 24, 2012
    Publication date: December 20, 2012
    Applicant: INNOVASCREEN, INC.
    Inventor: Roy Duncan
  • Publication number: 20120315651
    Abstract: Oogonial stem cell (OSC)-derived compositions, such as nuclear free cytoplasm or isolated mitochondria, and uses of OSC-derived compositions in autologous fertility-enhancing procedures are described.
    Type: Application
    Filed: April 13, 2012
    Publication date: December 13, 2012
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: JONATHAN L. TILLY, DORI C. WOODS
  • Publication number: 20120308580
    Abstract: There is provided at least one isolated TCR-like antibody or fragment thereof, wherein the antibody or fragment thereof is capable of specifically binding to at least one HBV derived peptide.
    Type: Application
    Filed: November 19, 2010
    Publication date: December 6, 2012
    Applicants: NATIONAL UNIVERSITY OF SINGAPORE, AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Antonio Bertoletti, Sastry Konduru Seetharama, Paul Anthony Macary, Soh Ha Chan, Chien Tei Too
  • Patent number: 8283132
    Abstract: The invention relates to a method for treating a biological cell (1) including a cytoskeleton (3) enveloped by a cell membrane (2). The method includes the following steps: the biological cell (1) and a tool (10) are mutually oriented such that the tool (10) comes into contact with the biological cell (1); the tool (10) and the biological cell (1) are displaced in relation to each other; and a gap is formed in the molecular composite of the cell membrane (2) of the biological cell (1). During the displacement of the tool (10), the cytoskeleton (3) of the biological cell (1) has a state of equilibrium. The invention also relates to a cell manipulator for carrying out the inventive method.
    Type: Grant
    Filed: November 2, 2005
    Date of Patent: October 9, 2012
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forshung e.V.
    Inventors: Günter Fuhr, Hagen Thielecke
  • Patent number: 8268617
    Abstract: DNAs, vectors, host cells and genetic constructs of antibodies, humanized antibodies, resurfaced antibodies, antibody fragments, derivatized antibodies, and conjugates of these molecules with cytotoxic agents, which specifically bind to and inhibit insulin-like growth factor-I receptor, antagonize the effects of IGF-I and are substantially devoid of agonist activity toward the insulin-like growth factor-I receptor. These molecules can be conjugated to cytotoxic agents for use in the treatment of tumors that express elevated levels of IGF-I receptor, such as breast cancer, colon cancer, lung cancer, ovarian carcinoma, synovial sarcoma and pancreatic cancer. These molecules can also be labeled for in vitro and in vivo diagnostic uses, such as in the diagnosis and imaging of tumors that express elevated levels of IGF-I receptor.
    Type: Grant
    Filed: August 26, 2009
    Date of Patent: September 18, 2012
    Assignee: ImmunoGen, Inc.
    Inventors: Rajeeva Singh, Daniel J. Tavares, Nancy E. Dagdigian
  • Publication number: 20120191163
    Abstract: A method of manipulating a living cell is disclosed. The method comprises, directing a pulsed optical field to at least one conductive nanoparticle present in the vicinity of the cell, so as to generate cavitations at or near the conductive nanoparticle at sufficient amount to effect at least one cell modification selected from the group consisting of cell-damage and cell-fusion.
    Type: Application
    Filed: January 19, 2012
    Publication date: July 26, 2012
    Applicant: Technion Research & Development Foundation Ltd.
    Inventors: Dvir Yelin, Limor Minai, Daniella Yeheskely-Hayon
  • Patent number: 8216842
    Abstract: The present invention provides methods of enhancing hybridoma fusion efficiencies through cell synchronization of the fusion partners, in order to aid in production of antibodies.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: July 10, 2012
    Assignee: Centocor Ortho Biotech Inc.
    Inventors: Jill Giles-Komar, Michael Rycyzyn, Gregory Bannish
  • Publication number: 20120164733
    Abstract: An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
    Type: Application
    Filed: February 24, 2012
    Publication date: June 28, 2012
    Inventors: George E. Seidel, Tae Kwang Suh, Kehuan Lu
  • Publication number: 20120151611
    Abstract: The present invention aims to provide a method for antibody preparation. The present invention is directed to a method for preparing an antibody-producing cell, which comprises the following steps: (1) transplanting metastatic cancer cells capable of expressing a target antigen into a non-human animal to ensure engraftment of the cancer cells in the animal; (2) immunizing the animal with the target antigen; and (3) collecting the antibody-producing cell from the immunized animal; as well as a method for preparing an antibody, which comprises collecting the antibody from the antibody-producing cell prepared by the above method.
    Type: Application
    Filed: August 24, 2011
    Publication date: June 14, 2012
    Inventors: Hiroyuki Satofuka, Masahiro Uchino, Shingo Hanaoka
  • Publication number: 20120142106
    Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
    Type: Application
    Filed: February 1, 2012
    Publication date: June 7, 2012
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Igor I. Slukvin, James A. Thomson, Maksym A. Vodyanyk, Maryna E. Gumenyuk
  • Publication number: 20120129260
    Abstract: Methods are provided for producing a human embryo capable of developing to the blastocyst stage. The method includes transferring a human somatic cell genome into a mature human oocyte by nuclear transfer and activating the oocyte, without removing the oocyte genome. Pluripotent human embryonic stem cells, and methods of obtaining these, are also provided.
    Type: Application
    Filed: November 22, 2011
    Publication date: May 24, 2012
    Applicant: THE NEW YORK STEM CELL FOUNDATION
    Inventors: Dietrich M. EGLI, Scott A. NOGGLE, Kevin C. EGGAN