Preparing Compound Containing A Carotene Nucleus (i.e., Carotene) Patents (Class 435/67)
  • Patent number: 11034968
    Abstract: The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell producing the product of interest in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the microalgal cell is a facultative heterotroph. The product of interest can be a microalgal biomass, a pigment, terpene, recombinant molecule, biogas, or a precursor thereof. In an embodiment, the culture medium comprises urea as a primary source of nitrogen. In one embodiment, the microalgal cell belongs to the order Chlamydomonadales. A method of identifying and isolating a microalgal cell having a preferred characteristic that is suitable for synthesis of a product of interest is also provided, the method comprising identifying and isolating a non-mutagenized or recombinant microalgal cell from a microalgal culture using a fluorescence activated cell sorting technique and/or a phototaxic response.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: June 15, 2021
    Assignee: Kuehnle AgroSystems, Inc.
    Inventors: Robert J. Schurr, Adelheid R. Kuehnle
  • Patent number: 10947574
    Abstract: The present invention provides a method for high yield and stable production of carotenoids through microbial culture. The present invention provides a method for culture of carotenoid-producing bacteria, which comprises culturing carotenoid-producing bacteria in a medium containing cobalt or a cobalt salt at a concentration of 0.005 ?mol/L to 20 ?mol/L.
    Type: Grant
    Filed: December 27, 2016
    Date of Patent: March 16, 2021
    Assignee: ENEOS CORPORATION
    Inventors: Tetsuhisa Yata, Hisashi Yoneda, Mitsutoshi Azuma, Kazuaki Hirasawa
  • Patent number: 10920230
    Abstract: Disclosed are methods of producing carotenoid compounds in a methylotrophic bacterial host cell. Such a host cell may be an unmodified Methylobacterium, spontaneous mutant, or transformed cell, any of which exhibit favorable properties, such as overproduction of carotenoid compounds, increased carbon flux, improved growth, or the production of additional nutrients, such as protein, vitamins, antioxidants, or fatty acids. Also disclosed are feed compositions for use in aquaculture, or as animal feed, or as human nutritional supplements containing processed or unprocessed biomass from such cells, as are methods of preparation of the feed compositions.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: February 16, 2021
    Assignee: KnipBio, Inc.
    Inventors: Larry F. Feinberg, Christopher J. Marx
  • Patent number: 10619170
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Patent number: 10487304
    Abstract: The present disclosure generally pertains to methods for the harvesting of biomass, in particular algae, from dilute aqueous suspension. In one embodiment, the method comprises the steps of: adjusting the pH of the dilute aqueous suspension to cause disruption of the algal cell walls and exposure of the hydrophobic tails of the algal lipid bilayer; adding at least one organic solvent to the dilute aqueous suspension; and mixing the dilute aqueous suspension. The method may also include the steps of: waiting for formation of an organic layer from the dilute aqueous suspension; and recovering the organic layer. The organic layer rises to the top of the dilute aqueous suspension, allowing for mechanical separation or decanting of the organic layer.
    Type: Grant
    Filed: January 3, 2017
    Date of Patent: November 26, 2019
    Assignee: Board of Trustees of the University of Alabama, for and on behalf of the University of Alabama in Huntsville
    Inventors: Chen Zhang, James E. Smith, Jr.
  • Patent number: 10294156
    Abstract: Various methods for making material capable of forming structural composites are disclosed. For example, a particular method may include growing an amount of cyanotic organisms, separating cell walls of the cyanotic organisms from internal portions of the cyanotic organisms to form a purified algae extract, the purified algae extract being from the internal portions of the cyanotic organisms, and applying an effective amount of alkaline water to the purified algae extract to form a balanced algae extract having little or no chemical reactivity, wherein a pH of the alkaline water is a function of a pH of the purified algae extract.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: May 21, 2019
    Inventor: Marcos Gonzalez
  • Patent number: 10240215
    Abstract: An object of the present invention is to provide a method for microbioiogically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product alter the end of culture in a biotin-free medium.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: March 26, 2019
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Hiroshi Satoh, Hisashi Yoneda, Tetsuhisa Yata, Mitsutoshi Azuma
  • Patent number: 10145803
    Abstract: This invention discloses a new class of pH indicators suitable for use in agricultural compounds. The class comprises naturally occurring substances which are extracts from grape skins, cabbage and lecithin. Safety concerns are now causing regulatory bodies to prohibit use of chemical pH indicators in compounds which are used for crops and animals. The naturally occurring compounds of this invention satisfy these concerns of the regulators and function as well as chemical indicators of the prior art.
    Type: Grant
    Filed: August 24, 2004
    Date of Patent: December 4, 2018
    Assignee: NUTRIAG LTD.
    Inventors: Martin David Bloomberg, Mark Stewart Houston-McMillan
  • Patent number: 9963731
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: May 8, 2018
    Assignee: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Patent number: 9695411
    Abstract: The present invention is directed towards genetic modification of native gene encoding for sucrose isomerase and isomaltulose synthase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare disaccharides such as isomaltulose and trehalulose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.
    Type: Grant
    Filed: April 17, 2013
    Date of Patent: July 4, 2017
    Assignee: Petiva Private Limited
    Inventors: Samir Kumar Roy, Saravanakumar Iyappan, Saju Varghese, Aparna Devi Addala, Milky Agarwal, Kanumuru Rahul Raju, Banibrata Pandey
  • Patent number: 9605323
    Abstract: An object of the present invention is to provide a method for microbiologically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product after the end of culture in a biotin-free medium.
    Type: Grant
    Filed: March 15, 2011
    Date of Patent: March 28, 2017
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Hiroshi Satoh, Hisashi Yoneda, Tetsuhisa Yata, Mitsutoshi Azuma
  • Patent number: 9562220
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: February 7, 2017
    Assignee: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Patent number: 9499802
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Pestalotiopsis virgatula.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: November 22, 2016
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Patent number: 9499801
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Pestalotiopsis virgatula.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: November 22, 2016
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Patent number: 9493792
    Abstract: The process for the production of the yellow pigment can include the steps of (a) culturing Bacillus sp. GSK07 bacteria; and (b) extracting the yellow pigment from the bacterial culture using a solvent. The solvent for extraction can be an alcohol, e.g., ethanol, methanol, or both ethanol and methanol. The pigment includes D-limonene.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: November 15, 2016
    Assignee: KING SAUD UNIVERSITY
    Inventors: George Seghal Kiran, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Joseph Selvin
  • Patent number: 9434898
    Abstract: This invention relates to compositions comprising high lipid content algae and methods of making and utilizing the same. In particular, the invention relates to high lipid content algae biomass and algal lipid materials derived from the same, methods of making the same, as well as to biofuels (e.g., biodiesel) and dietary compositions (e.g., animal feeds) comprising or made from the same. Compositions and methods of the invention find use in a variety of applications including biofuel, dietary (e.g., human and animal nutrition), therapeutic as well as research applications.
    Type: Grant
    Filed: July 13, 2012
    Date of Patent: September 6, 2016
    Assignee: Alltech, Inc.
    Inventors: Kyle A. Raney, Rebecca A. Timmons
  • Patent number: 9347035
    Abstract: Culture compositions comprising Haematococcus pluvialis and hydrogen peroxide are described herein. The compositions comprise a concentration of hydrogen peroxide based on the stage of the cells in the culturing process to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 10, 2015
    Date of Patent: May 24, 2016
    Assignee: Heliae Development LLC
    Inventors: Laura Carney, Kristine Sorensen
  • Patent number: 9271935
    Abstract: The present invention encompasses a carotenoid composition, a process for producing a carotenoid composition, and methods of use thereof.
    Type: Grant
    Filed: December 13, 2013
    Date of Patent: March 1, 2016
    Assignee: Novus International, Inc.
    Inventors: Gary Reznik, Joan Carles Ferrater Martorell, David Ribera, Antonio Viso, Juan Antonio Fernandez, Delfin Ferrus, Scott Hine
  • Patent number: 9192588
    Abstract: An object of the present invention is to provide zeaxanthin-enriched poultry eggs containing zeaxanthin at high concentrations. The present invention specifically relates to zeaxanthin-enriched poultry eggs obtained by feeding poultry with a poultry feedstuff containing zeaxanthin-producing bacteria.
    Type: Grant
    Filed: February 17, 2012
    Date of Patent: November 24, 2015
    Assignee: JX NIPPON OIL & ENERGY CORPORATION
    Inventors: Yuki Kawashima, Hidetada Nagai, Michihisa Ikarashi
  • Patent number: 9113607
    Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: August 25, 2015
    Assignee: Heliae Development, LLC
    Inventors: Laura T. Carney, Kristine Sorensen
  • Patent number: 9102952
    Abstract: Described is a method for the enzymatic production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol as well as the use of an enzyme which is capable of catalyzing the decarboxylation of mevalonate for the production of isoprenol from mevalonate. Furthermore described is the use of mevalonate as a starting material for the production of isoprenol in an enzymatically catalyzed reaction.
    Type: Grant
    Filed: December 22, 2009
    Date of Patent: August 11, 2015
    Assignees: Global Bioenergies SA, Scientist of Fortune
    Inventors: Philippe Marliere, Maria Anissimova, Romain Chayot, Marc Delcourt
  • Publication number: 20150147780
    Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Eugene Dinescu, Vincent Dinescu
  • Publication number: 20150125903
    Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Patent number: 8999664
    Abstract: The present invention provides a salmon flesh color improving method and a salmon flesh color improving feed which are usable for producing a salmon having a mild reddish orange flesh color close to the flesh color of wild fish, and a salmon and fish flesh thereof produced by such a flesh color improving method. The present invention also provides a method for producing a salmon having a mild reddish orange flesh color, comprising cultivating the salmon with a feed comprising a carotenoid colorant mixed therein, the carotenoid colorant comprising at least astaxanthin, phoenicoxanthin, canthaxanthin and adonixanthin.
    Type: Grant
    Filed: September 14, 2007
    Date of Patent: April 7, 2015
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Akira Tsubokura, Hisashi Yoneda
  • Publication number: 20150093776
    Abstract: A method for producing metabolites of Crocus sativus (C. sativus) includes (i) selecting a cell line of C. sativus that produces one or more saffron metabolites in cell suspension culture, and (ii) growing the selected cell line in a suspension cell culture to produce the saffron metabolite.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 2, 2015
    Inventors: Sung-Yong H. Yoon, Raymond E. B. Ketchum, Colby G. Caldwell
  • Patent number: 8993282
    Abstract: The present invention provides a method for producing a carotenoid, which comprises culturing a carotenoid-producing bacterium in an amino acid-supplemented medium, and collecting the carotenoid from the resulting cultured product, wherein the amino acid is at least one selected, from the group consisting of glutamic acid, aspartic acid, glutamine, asparagine, alanine, glycine, serine, threonine, arginine, tyrosine, proline, phenylalanine and leucine, and salts thereof.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: March 31, 2015
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Akira Tsubokura, Hiroshi Satou, Tetsuhisa Yata
  • Publication number: 20150079627
    Abstract: The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
    Type: Application
    Filed: March 28, 2013
    Publication date: March 19, 2015
    Inventors: Thomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
  • Patent number: 8980795
    Abstract: Novel compounds of formula (I) their use as germination trap for parasitic weeds, for the regulation of branching, tillering and root development, for enhancement of cambium growth, for the regulation of hyphal growth of mycorrhizal fungi and compositions comprising compounds of formula (I) and insecticides and/or fungicides.
    Type: Grant
    Filed: April 25, 2012
    Date of Patent: March 17, 2015
    Assignee: King Abdullah University of Science and Technology
    Inventors: Salim Al-Babili, Adrian Alder
  • Publication number: 20150037839
    Abstract: Novel strains of microalgae belonging to the Scenedesmus genus enable the production of lipids, in particular lutein, in mixotrophic mode, as well as a method for selecting and culturing the strains using a variable and/or discontinuous supply of light, in particular in the form of flashes.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 5, 2015
    Applicant: FERMENTALG
    Inventors: Khadidja Romari, Francois Godart, Pierre Calleja
  • Publication number: 20150037838
    Abstract: New strains of protists belonging to the Schizochytrium genus, allow high-yield production of lipids and carotenoids, in particular of astaxanthin and docosahexaenoic acid (DHA), in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 5, 2015
    Applicant: FERMENTALG
    Inventors: Khadidja Romari, Adeline Le Monnier, Cyril Rols, Cindy Merlet, Julien Pagliardini, Pierre Calleja, Claude Gudin
  • Publication number: 20150005534
    Abstract: Provided herein are recombinant cells (e.g., recombinant bacteria or plant, insect, mammalian, and yeast cells) containing a nucleic acid encoding a CYP97A protein or a nucleic acid encoding a CYP97B protein; a nucleic acid encoding a CYP97C protein; a nucleic acid encoding a geranylgeranyl pyrophosphate synthase protein; a nucleic acid encoding a phytoene synthase protein; a nucleic acid encoding a phytoene desaturase protein; a nucleic acid encoding a lycopene ?-cyclase protein; and a nucleic acid encoding a lycopene ?-cyclase protein. Also provided are methods of producing lutein that include culturing these recombinant cells (e.g., recombinant bacteria and yeast cells), and methods of generating these recombinant cells (e.g., recombinant bacteria and yeast cells). Also provided is lutein produced by these methods, and pharmaceutical compositions, food supplements, food products, and cosmetic compositions that contain lutein produced by these methods.
    Type: Application
    Filed: February 5, 2013
    Publication date: January 1, 2015
    Inventors: Eleanor T Wurtzel, Rena Quinlan
  • Patent number: 8916359
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: November 30, 2010
    Date of Patent: December 23, 2014
    Assignees: Novozymes A/S, Novozymes North America, Inc.
    Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
  • Patent number: 8911965
    Abstract: Enhanced yields of photosynthetically fixed carbon produced by hypersaline photosynthetic algae are provided by co-culturing with a halophilic archaea. Further, methods are provided to control harvesting of desired metabolic products from hypersaline photosynthetic algae by controlling caspase activity.
    Type: Grant
    Filed: April 22, 2011
    Date of Patent: December 16, 2014
    Assignee: Institute for Systems Biology
    Inventors: Nitin S. Baliga, Monica V. Orellana, Kenia Whitehead, W. Lee Pang
  • Patent number: 8911966
    Abstract: The present invention pertains to a method for the efficient production of carotenoids. In particular, the present invention is directed to a method for producing carotenoid and carotenoid containing cells, especially astaxanthin and astaxanthin-containing cells, by generating mutant microorganisms belonging to the photoautotrophic algae of the Class Chlorophyceae and culturing same. The present invention further relates to methods of generating microorganisms producing high yields of carotenoids, in particular astaxanthin, products containing said microorganisms or said carotenoids, and the use of said carotenoids produced by the microorganisms according to the present invention and deposited mutant strains generated from said microorganisms.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: December 16, 2014
    Assignee: Cognis IP Management GmbH
    Inventors: Lucia Martin, Irina Obraztsova, Baldomero Cordero, Angeles M. Vargas
  • Publication number: 20140349338
    Abstract: The present invention relates to novel bacteria and the uses thereof. The invention particularly relates to bacteria having a metabolic pathway ratio between Pentose phosphate and glycolysis greater than 0.5, and their uses in the chemical, pharmaceutical or agro-chemical industries, e.g., for producing compounds of industrial interest.
    Type: Application
    Filed: December 21, 2012
    Publication date: November 27, 2014
    Applicant: DEINOVE
    Inventors: Jean-Paul Leonetti, Juha-Pekka Pitkanen, Paula Jouhten
  • Patent number: 8883443
    Abstract: A method for microbiologically producing zeaxanthin at high concentration and low cost while suppressing production of gluconic acid is presented. Specifically, a method for producing carotenoids containing zeaxanthin by culturing a bacterium producing carotenoids containing zeaxanthin in a medium containing biotin is provided.
    Type: Grant
    Filed: March 29, 2011
    Date of Patent: November 11, 2014
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Hiroshi Satoh, Hisashi Yoneda, Tetsuhisa Yata, Mitsutoshi Azuma
  • Publication number: 20140315243
    Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase, a thermostable protease, and optionally a carbohydrate-source generating enzyme and/or pullulanase, are present and/or added during liquefaction. The invention also relates to compositions suitable for use in a process of the invention.
    Type: Application
    Filed: November 30, 2012
    Publication date: October 23, 2014
    Inventors: Randall Deinhammer, Joyce Craig, Tomoko Matsui, Shinobu Takagi, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong
  • Patent number: 8859228
    Abstract: The invention relates to a novel method for the production of ?-carotene from submerged cultures of mucoral fungi such as Blakeslea, Choanephora or Phycomyces by adding lectin to the culture medium and performing pH control once fermentation has started. The method involves ?-carotene recovery stage that makes it possible to simplify the process, optimize yields and increase purification of the product.
    Type: Grant
    Filed: July 18, 2001
    Date of Patent: October 14, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Javier Costa Perez, Antonio Estrella Castro, Ana Teresa Marcos Rodriguez, J. Emiliano Gonzalez De Prado, Enrique R. Peiro Cezon, Alfonso Collados De La Vieja, Manuel Esteban Morales
  • Publication number: 20140295491
    Abstract: A duckweed hydrolysate is provided. A method for producing carotenoids comprising the incubation of microorganisms with the duckweed is also provided.
    Type: Application
    Filed: April 1, 2013
    Publication date: October 2, 2014
    Applicant: Lemnaceae Fermentation, Inc.
    Inventor: Ming-Hsi Chiou
  • Publication number: 20140287924
    Abstract: Novel compounds of formula (I) their use as germination trap for parasitic weeds, for the regulation of branching, tillering and root development, for enhancement of cambium growth, for the regulation of hyphal growth of mycorrhizal fungi and compositions comprising compounds of formula (I) and insecticides and/or fungicides.
    Type: Application
    Filed: April 25, 2012
    Publication date: September 25, 2014
    Inventors: Salim Al-Babili, Adrian Alder
  • Patent number: 8828697
    Abstract: A method of producing lycopene, with high productivity by means of a recombinant bacterial strain includes preparing the recombinant vector containing genes encoding proteins, which are required for lycopene biosynthesis. The genes involved in lycopene biosynthesis are crtE with the nucleotide SEQ ID NO: 8, crtB with the nucleotide SEQ ID NO: 3 and crtI with the nucleotide SEQ ID NO: 5 of the Sequence List. The said recombinant vector is transformed into Escherichia coli (hereafter E. coli). The E. coli transformant is cultured to recover lycopene from the culture medium.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: September 9, 2014
    Assignee: Amicogen
    Inventors: Nahm Ryune Cho, Min Soo Park, Dong Hyun Lee, Ho Seung Chung, Jong Keun Kim
  • Patent number: 8815567
    Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of co-producing coenzyme Q10 and at least one ?-3/?-6 polyunsaturated fatty acid are provided. The strains may also be engineered to co-produce at least one C40 carotenoid. Methods of using the antioxidant products obtained (e.g., biomass and/or pigmented oils) in food and feed applications are also provided.
    Type: Grant
    Filed: November 25, 2008
    Date of Patent: August 26, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventor: Rick W. Ye
  • Publication number: 20140206035
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: July 24, 2014
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20140187829
    Abstract: The present invention provides a method for producing and purifying ?-carotene by Blakeslea trispora fermentation and use thereof. The method comprises the following steps: a) separately inoculating the Blakeslea trispora strains onto a PDA culture medium so as to obtain a spore suspension; b) propagating spores in a seeding tank so as to obtain seeds for fermentation; c) inoculating the seeds for fermentation onto a fermenter and fermenting said seeds; d) adjusting the fermentation liquid to be basic by using an organic or inorganic base, and filtering so as to obtain wet mycelia; e) treating the wet mycelia with a hydrophobic non-polar organic solvent; f) mixing the wet mycelia with an organic solvent of ester and obtaining a concentrated solution by extracting; g) adding a saturated monohydric alcohol into the concentrated solution, and filtering and crystallizing so as to obtain pure ss-carotene. The content of the ss-carotene in the present invention exceeds 96%, and the yield is above 85%.
    Type: Application
    Filed: May 15, 2012
    Publication date: July 3, 2014
    Applicant: Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutic- al Factory
    Inventors: Xinde Xu, Mingqing Jiao, Dong Shao, Bin Shao, Leiming Yu
  • Publication number: 20140170700
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Application
    Filed: June 8, 2012
    Publication date: June 19, 2014
    Applicant: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Publication number: 20140154732
    Abstract: The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA1 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in plants, e.g., green microalgae, under bright sunlight conditions.
    Type: Application
    Filed: February 5, 2014
    Publication date: June 5, 2014
    Applicant: The Regents of the University of California
    Inventors: Anastasios MELIS, Mautusi MITRA
  • Patent number: 8741599
    Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproduc
    Type: Grant
    Filed: November 27, 2006
    Date of Patent: June 3, 2014
    Assignee: BASF SE
    Inventors: Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
  • Patent number: 8735137
    Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: May 27, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Seung-Pyo Hong, John E. Seip, Quinn Qun Zhu
  • Publication number: 20140134672
    Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.
    Type: Application
    Filed: July 5, 2012
    Publication date: May 15, 2014
    Inventors: Max B. Tuttman, David A. St. Angelo