Indirect Assay Patents (Class 435/7.95)
  • Patent number: 9884899
    Abstract: The application provides methods for determining a patient's risk for developing fibrosis or a fibrosis-related disorder. Concentrations of C reactive protein (CRP) and serum amyloid protein (SAP) are measured from a biological sample to determine the SAP-to-CRP ratio. This ratio can then be compared with one or more SAP-to-CRP reference ratios to determine a patient's risk for developing a fibrosis related disorder. The diagnostic methods can also be used to determine the severity of fibrosis in a patient afflicted with such a disease. Furthermore, methods for treating patients having a fibrosis-related disorder are provided. For example, a patient that has a lower SAP-to-CRP ratio than one or more reference values may be treated with an SAP agonist and/or CRP antagonist to treat or prevent a fibrosis disorder. The methods may further comprise determining the R131/H131 polymorphism of Fc?RIIA as a risk factor for developing fibrosis or a fibrosis-related disorder.
    Type: Grant
    Filed: June 27, 2008
    Date of Patent: February 6, 2018
    Assignee: Promedior, Inc.
    Inventors: Mark L. Lupher, Jr., Teresa K. Surowy, Shawn Li, David Paul Hesson, Michael Scott Kramer
  • Patent number: 9810694
    Abstract: The present invention is drawn to methods for detection of the fully human antibodies infiltrated into a solid tissue, and to optimize methods for generating, selecting, and expanding monoclonal antibodies to large numbers for antibody-based therapy and diagnosis. The invention is exemplified in a number of implementations and applications, some of which are summarized below and throughout the specification. In one aspect, the invention is directed to methods for identifying potentially therapeutic fully human antibodies that have infiltrated a solid tissue comprising the following steps: (a) detecting and sorting into one or more subsets a sample of antibodies from an accessible tissue of an individual; (b) characterization of the fully human antibodies; (c) production and purification of the fully human antibody; (d) assessment of the therapeutic and diagnostic activity of fully human antibody.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: November 7, 2017
    Inventors: Gennady Bratslavsky, Ilya Tsimafeyeu
  • Patent number: 9594078
    Abstract: The present application discloses an analyte detection apparatus having at least one reservoir area and a wicking membrane, wherein a labeled specific binding partner is impregnated on the reservoir area; and a region on the wicking membrane where at least one chemical component is immobilized.
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: March 14, 2017
    Assignee: ACCESS BIO, INC.
    Inventors: Young Ho Choi, Jaean Jung
  • Patent number: 9417218
    Abstract: This invention relates to methods of analysis, and in particular to methods for the preliminary fractionation of samples in which low abundance molecules of interest, for example proteins, polysaccharides or fatty acids, are present together with more abundant molecules of little or no interest. In particular, the invention relates to methods of depletion of high abundance proteins from biological samples. Products and kits for use in the method are also disclosed, and form part of the invention. In one aspect, the invention provides a method of depleting a high-abundance molecule from a biological sample, comprising the steps of a) subjecting the sample to affinity depletion using an affinity support with high affinity for a high abundance molecule, and/or b) immunodepletion using an affinity support coupled to an antibody directed against whole or previously fractionated plasma or serum.
    Type: Grant
    Filed: June 11, 2010
    Date of Patent: August 16, 2016
    Assignee: THERAPEUTICSMD, INC.
    Inventors: Gregory E. Rice, Michael Quinn
  • Patent number: 9341624
    Abstract: Devices and methods for the detection of target molecules are disclosed. Devices and methods for detecting food-borne target molecules are also disclosed.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: May 17, 2016
    Assignee: Invisible Sentinel, Inc.
    Inventors: Nicholas A. Siciliano, Martin Joseph Bouliane
  • Patent number: 9261513
    Abstract: An isolated antibody having a specific binding affinity for a polypeptide comprising the amino acid sequence HTEGKP (SEQ ID NO: 2) phosphorylated at threonine is described. The antibody may be used as biomarker for mitotic cells. A method for using the antibody includes contacting a cell with the antibody and detecting antibody bound to the cell as an indicator of the cell being in the mitotic state. A reagent kit comprising the antibody is also described.
    Type: Grant
    Filed: July 14, 2014
    Date of Patent: February 16, 2016
    Assignee: Florida State University Research Foundation, Inc.
    Inventors: Myra Hurt, Raed Rizkallah
  • Patent number: 9229012
    Abstract: Provided herein are monoclonal antibodies with a high binding affinity to isoforms of alpha 1-antitrypsin (A1AT), hybridoma cells producing the same, and their uses in diagnosing and/or detecting endometriosis from a serum sample of a subject suspicious of having endometriosis or a subject under health examination.
    Type: Grant
    Filed: July 15, 2013
    Date of Patent: January 5, 2016
    Assignees: Taipei Medical University, Academia Sinica
    Inventors: Wei-Chung Yang, Hwei-Jiung Wang, Shui-Tsung Chen, Ken-Fen Lu, Ming-Chi Peng
  • Patent number: 9103841
    Abstract: Provided is a method of measuring the presence and/or the amount of glucagon-like peptide-1 (GLP-1) in a sample, which method is characterized by comprising the step of treating the sample in advance with an acidic solution, and a kit of measuring the presence and/or an amount of GLP-1 in a sample, the kit containing (a) the acidic solution, (b) an antibody specific to GLP-1, and (c) an instruction manual.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: August 11, 2015
    Assignee: LSI MEDIENCE CORPORATION
    Inventors: China Sakai, Shigeru Tashiro
  • Patent number: 9057723
    Abstract: The present invention relates to a method for detecting an analyte (10) in a sample, comprising the steps of: providing a transducer (3) having a pyroelectric or piezoelectric element and electrodes which is capable of transducing a change in energy to an electrical signal, a first reagent (9) immobilized on the transducer, the first reagent having a binding site which is capable of binding the analyte or a derivative of the analyte, exposing the sample to the transducer thereby allowing the analyte or a derivative of the analyte to bind to the first reagent to form a first reagent-analyte complex (13); introducing a second reagent (11), the second reagent having a binding site which is capable of selectively binding the first reagent-analyte complex, wherein the second reagent has a label (12) attached thereto which is capable of absorbing electromagnetic radiation to generate energy by non-radiative decay; irradiating the sample with electromagnetic radiation; transducing the energy generated into an electr
    Type: Grant
    Filed: March 31, 2009
    Date of Patent: June 16, 2015
    Assignee: Vivacta Ltd.
    Inventors: Timothy Joseph Nicholas Carter, Steven Andrew Ross
  • Patent number: 9028801
    Abstract: The invention relates to a method of diagnosis of vCJD in a diagnostic sample of a valid body tissue taken from a human subject, which comprises detecting an increased concentration of a protein in the diagnostic sample, compared with a sample of a control human subject, the protein being: beta-actin (SwissProt Acc. No. P60709), apolipoprotein A-IV precursor (SwissProt Acc. No. P06727); haptoglobin beta-chain consisting of residues 162-406 (SwissProt Acc. No. P00738); haemoglobin beta chain (SwissProt Acc. No. P02023); or alpha-1-antitrypsin (SwissProt Acc. No. P01009); or a decreased concentration of a protein in the diagnostic sample, compared with a sample of a control, normal human subject, the protein being plasma protease (C1) inhibitor precursor (SwissProt Acc. No. P05155); complement component 1, s sub-component (SwissProt Acc. No. P09871); butyrylcholinesterase precursor (SwissProt Acc. No. P06276); complement component C4B (SwissProt Acc. No. P01028); lumican (SwissProt Acc. No.
    Type: Grant
    Filed: December 7, 2005
    Date of Patent: May 12, 2015
    Assignees: Electrophoretics Limited, Medical Research Council, University College London
    Inventors: Malcolm Andrew Ward, John Collinge, Graham Stuart Jackson, Emma McGregor, Nicola Louise Leeds, James Campbell, Jules Arthur Westbrook, Helen Louise Byers
  • Publication number: 20150093769
    Abstract: Products and methods for the research, diagnosis, risk assessment, course monitoring, treatment and prophylaxis of various metabolic disorders and their early forms, concomitant diseases and secondary diseases are provided. Metabolic diseases include, for example, metabolic syndrome, non-insulin-dependent diabetes, (type II diabetes), insulin resistance, obesity (adiposis), in addition to diseases that are associated with disorders of the fatty acid metabolism. Methods may include analysis of the concentration of the adipocytic form of the fatty acid binding protein (A-FABP, FABP 4, P2) in various bodily fluids.
    Type: Application
    Filed: September 10, 2014
    Publication date: April 2, 2015
    Applicant: BIOVENDOR LABORATORY MEDICINE, INC.
    Inventor: VIKTOR RUZICKA
  • Publication number: 20150079583
    Abstract: One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a substrate, a hermetically-sealed, thermally decoupled central reaction zone associated with the substrate, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the substrate and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the substrate. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet.
    Type: Application
    Filed: August 22, 2014
    Publication date: March 19, 2015
    Inventors: Franz Baudenbacher, Raymond Mernaugh, John Mayo, Brad Lubbers
  • Publication number: 20150072879
    Abstract: The present invention provides methods and systems to predict and diagnose inflammatory bowel disease (IBD) and subtypes such as ulcerative colitis (UC) and Crohn's disease (CD) by detecting the presence, absence, level, and/or genotype of one or more sero-genetic-inflammation markers. Advantageously, with the present invention, it is possible to provide a diagnosis of IBD versus non-IBD, to rule out IBD that is inconclusive for CD and UC, and to differentiate between CD and UC with increased accuracy.
    Type: Application
    Filed: March 28, 2014
    Publication date: March 12, 2015
    Applicant: NESTEC S.A.
    Inventors: Fred Princen, Steven Lockton, Lisa J. Croner, Frederick A. Fletcher, Thomas Stockfisch, Sharat Singh
  • Publication number: 20150071927
    Abstract: Disclosed are antibodies, or binding fragments thereof, that specifically bind to human HER2 and human IGF-IR. Also provided are nucleic acid molecules encoding the disclosed antibodies and binding fragments and vectors and host cells containing these nucleic acid molecules. The disclosure also provides methods of inhibiting cancer cell growth and metastasis in a mammal using the antibodies described herein, as well as compositions containing the antibodies, nucleic acid molecules encoding the antibodies, and host cells and vectors comprising the nucleic acid molecules. The disclosure also features the use of the polypeptides to detect the presence of HER2 and IGF-IR in a mammal, and epitopes that can be used as cancer vaccine immunogens.
    Type: Application
    Filed: September 11, 2014
    Publication date: March 12, 2015
    Inventor: Mei-Yun Zhang
  • Patent number: 8962257
    Abstract: The invention relates to methods of diagnosing the severity of heart failure or a cardiac dysfunction in a subject. The invention further relates to monitoring the severity of heart failure in a subject and determining the prognosis of a subject that has suffered from heart failure. This invention also relates methods to identify patients at risk for cardiac dysfunction when exposed to cardiotoxic chemotherapy agents.
    Type: Grant
    Filed: March 8, 2011
    Date of Patent: February 24, 2015
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Bonnie Ky, Thomas Cappola, Douglas B. Sawyer
  • Patent number: 8962264
    Abstract: The present invention provides a method of detecting dermatophyte, which does not require a complicated operation such as an enzyme treatment and a heat treatment. The present invention provides a method of detecting dermatophyte, including a step of extracting a dermatophyte component from a sample with a treatment liquid containing a non-ionic surfactant or a zwitterionic surfactant, and a kit for diagnosing dermatophyte infection, containing a treatment liquid comprising the above surfactant, and an antibody specifically recognizing a dermatophyte component, which are housed in separate containers.
    Type: Grant
    Filed: March 19, 2007
    Date of Patent: February 24, 2015
    Assignees: National University Corporation University of Fukui, JNC Corporation
    Inventor: Sakon Noriki
  • Patent number: 8956823
    Abstract: An anti-antibody reagent for use in a competitive or sandwich simplex or multiplex assay, said reagent comprising one or more labeled anti-antibodies for the primary antibodies to be determined in the assay, the reagent further comprising a corresponding unlabeled anti-antibody in an excess or near excess concentration with respect to their binding partners.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: February 17, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: William F. Link, Renato B. Del Rosario, Randy Sweet, David L. King
  • Publication number: 20150037813
    Abstract: The present invention provides a means and a method for specifically measuring a substance such as a small substance with high sensitivity by a sandwich method. Specifically, the present invention provides an antibody capable of specifically binding to an affinity complex and a method of measuring of the affinity complex comprising measuring the affinity complex using the antibody capable of specifically binding to the affinity complex. The antibody of the present invention may be a full-length antibody. The antibody of the present invention may also have a region derived from an immunoglobulin from an animal having an ability of gene conversion (e.g., a complementarity-determining region, a framework region, or a variable region). Examples of at least one factor that constitutes the affinity complex include a small substance or a protein (e.g., antibody).
    Type: Application
    Filed: July 4, 2012
    Publication date: February 5, 2015
    Applicant: FUJIREBIO INC.
    Inventor: Yoshie GOISHI
  • Patent number: 8927225
    Abstract: Disclosed is an assay (method) to quantify the amounts of catecholamine-O-methyltransferase (COMT) protein in samples, such as extracts from cell cultures, body fluids, tissues, and environmental samples. It uses novel agents (anti-NE, COMT-NE, or COMT-epitope-NE) in combination with two previously described agents (anti-COMT and COMT) in a competitive ELISA system to achieve this aim.
    Type: Grant
    Filed: September 8, 2009
    Date of Patent: January 6, 2015
    Assignee: The University of Hong Kong
    Inventors: Shu Leong Ho, Wing Lok Ho, David Boyer Ramsden
  • Publication number: 20150005192
    Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.
    Type: Application
    Filed: September 12, 2014
    Publication date: January 1, 2015
    Applicant: Cambridge Enterprise Limited
    Inventors: Sabine Bahn, Paul Guest, Laura Harris, Yishai Levin, Divya Krishnamurthy
  • Patent number: 8911952
    Abstract: This invention relates generally to integrin ligand discovery and to a method of integrin ligand discovery base upon induction of ligand-induced epitopes. Such ligands have the potential to be active agent as anti-inflammatory, anti-angiogenesis and/or anti-thrombotic agents and for the treatment of integrin mediated diseases and/or conditions.
    Type: Grant
    Filed: March 23, 2011
    Date of Patent: December 16, 2014
    Assignee: STC. UNM
    Inventors: Alexandre Chigaev, Larry A. Sklar
  • Publication number: 20140363450
    Abstract: Low levels of antibodies reactive with oxidised Cardiolipin (oxCL) in mammals are related to an increased risk of developing cardiovascular diseases, auto-immune diseases or inflammatory conditions. High levels can have a protective function and in general there is a negative association between manifestations of these conditions and antibodies against oxCL. Thus, based on their relations methods of monitoring, determining and diagnosing as well as methods of immunisation and therapy of these diseases and conditions are provided.
    Type: Application
    Filed: August 12, 2013
    Publication date: December 11, 2014
    Applicant: MEDIRISTA BIOTECHNOLOGIES AB
    Inventor: Johan FROSTEGÅRD
  • Publication number: 20140349324
    Abstract: The present invention relates to materials and methods concerning the IL-1 receptor family protein ST2. Use of soluble ST2 as a marker for cardiovascular disease or disease outcome is provided, in particular as a marker of the risk of mortality.
    Type: Application
    Filed: December 14, 2012
    Publication date: November 27, 2014
    Inventor: Yves Levy
  • Patent number: 8835125
    Abstract: Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: September 16, 2014
    Assignee: Affymetrix, Inc.
    Inventors: Robert G. Kuimelis, Glenn H. McGall, Stephen P. A. Fodor
  • Publication number: 20140248272
    Abstract: The present invention relates to proteins and/or fragments and derivatives thereof and their use as vaccines and in biotechnological methods. The vaccines particularly include immunogenic proteins in Treponema spp. isolated from digital dermatitis in cattle. The present invention further relates to antibodies raised against said proteins or fragments thereof, and the use of said proteins in diagnostic methods in which antibodies are detected as a sign of digital dermatitis in cattle.
    Type: Application
    Filed: March 4, 2014
    Publication date: September 4, 2014
    Inventors: Anna ROSANDER, Märit PRINGLE
  • Patent number: 8822168
    Abstract: The present invention includes assays and kits for detecting the assembly of an RNA binding protein-RNA complex and for detecting the activity of an RNA binding protein.
    Type: Grant
    Filed: March 10, 2006
    Date of Patent: September 2, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Gideon Dreyfuss, Lili Wan, Elizabeth Ottinger
  • Patent number: 8822169
    Abstract: There is disclosed a pharmaceutical composition and method for treating sepsis, including septic shock and ARDS (acute respiratory distress syndrome), comprising administering an effective amount of a HMG1 antagonist. There is further disclosed a diagnostic method for monitoring the severity or potential lethality of sepsis or septic shock, comprising measuring the serum concentration of HMG1 in a patient exhibiting or at risk of exhibiting sepsis or septic shock symptoms. Lastly, there is disclosed a pharmaceutical composition and method for effecting weight loss or treating obesity, comprising administering an effective amount of HMG1 or a therapeutically active HMG1 fragment.
    Type: Grant
    Filed: February 22, 2012
    Date of Patent: September 2, 2014
    Assignee: The Feinstein Institute for Medical Research
    Inventors: Kevin J. Tracey, Haichao Wang
  • Publication number: 20140234878
    Abstract: Provided are compositions for diagnosing acute myocardial infarction including a preparation for measuring an amount of a substance P (SubP) or neuropeptide Y (NpY), kits for diagnosing acute myocardial infarction comprising the composition, and methods of diagnosing acute myocardial infarction based on the amount of the substance P or the neuropeptide Y.
    Type: Application
    Filed: July 22, 2013
    Publication date: August 21, 2014
    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Min-Jung KANG, Hyojeong HAN, Byung Hwa JUNG
  • Publication number: 20140221245
    Abstract: The invention relates to a method of detecting a disease state or disease susceptibility in a mammalian subject which comprises detecting an antibody in a test sample comprising a bodily fluid from said mammalian subject wherein said antibody is a biological marker of a disease state or disease susceptibility, the method comprising: (a) contacting said test sample with a plurality of different amounts of an antigen specific for said antibody, (b) detecting the amount of specific binding between said antibody and said antigen, (c) plotting or calculating a curve of the amount of said specific binding versus the amount of antigen for each amount of antigen used in step (a) and (d) determining the presence or absence of said disease state or disease susceptibility based upon the amount of specific binding between said antibody and said antigen at each different antigen concentration used.
    Type: Application
    Filed: April 7, 2014
    Publication date: August 7, 2014
    Applicant: ONCIMMUNE LTD.
    Inventors: John Forsyth Russell Robertson, Anthony Barnes, Andrea Murray, Caroline Chapman
  • Patent number: 8765393
    Abstract: An affinity ligand-matrix conjugate of the structure Z-Spacer-[NX-A]m-NY-A-NK2 is useful for the isolation, separation, purification, characterization, identification or quantification of endotoxins in an aqueous system, wherein m is an integer of at least one; each A independently represents an optionally substituted linear, branched or cyclic saturated hydrocarbon chain containing 1 to 6 carbon atoms; each X independently represents hydrogen or alkyl; Y is X or A-NX2; and Z is a support matrix attached to the ligand through an optional spacer arm (Spacer).
    Type: Grant
    Filed: May 22, 2003
    Date of Patent: July 1, 2014
    Assignee: Prometic Biosciences Ltd
    Inventors: Steven James Burton, Tadeusz Podgorski, Saji S. Eapen
  • Publication number: 20140178332
    Abstract: The present invention encompasses IL-12p40 binding proteins, particularly antibodies that bind human interleukin-12 (hIL-12) and/or human IL-23 (hIL-23). Specifically, the invention relates to antibodies that are chimeric, CDR grafted and humanized antibodies. Preferred antibodies have high affinity for hIL-12 and/or hIL-23 and neutralize h IL-12 and/or hIL-23 activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. Method of making and method of using the antibodies of the invention are also provided. The antibodies, or antibody portions, of the invention are useful for detecting hIL-12 and/or hIL-23 and for inhibiting hIL-12 and/or hIL-23 activity, e.g., in a human subject suffering from a disorder in which hIL-12 and/or hIL-23 activity is detrimental.
    Type: Application
    Filed: January 13, 2014
    Publication date: June 26, 2014
    Applicant: ABBVIE INC.
    Inventors: Susan E. Lacy, Emma Fung, Jonathan P. Belk, Richard W. Dixon, Michael Roguska, Paul R. Hinton, Shankar Kumar
  • Publication number: 20140178903
    Abstract: The present invention, fitted in the medical-clinical sector, shows a method for monitoring the ingestion of gluten by measuring protein/gluten peptides present in fecal samples with antibodies against immunogenic peptides resistant to gastrointestinal digestion. The presence or absence of said immunogenic peptides is controlled by immunological assays based on reactive antibodies against immunogenic gluten peptides that are resistant to proteolysis. These assays may be quantitative techniques as ELISAs, or qualitative as rapid immunochromatographic assays, immunoblots, etc. These measures may also be applied to verify compliance with the gluten-free diet, to improve diagnosis in cases of refractory or severe symptoms of celiac disease, in cases in which a gluten-free diet is supposedly being respected, or to clinical research on the effectiveness of enzymatic therapies related with prolamin detoxification.
    Type: Application
    Filed: December 28, 2011
    Publication date: June 26, 2014
    Applicant: UNIVERSIDAD DE SEVILLA
    Inventors: Carolina Sousa Martin, Isabel Comino Montilla, Ana Real Calderon, Santiago Vivas Alegre, Angel Cebolla Rammirez
  • Patent number: 8759114
    Abstract: Peptides useful in determining the presence of autoantibodies in patients suffering from rheumatoid arthritis are disclosed.
    Type: Grant
    Filed: June 17, 2013
    Date of Patent: June 24, 2014
    Assignee: Stichting Voor de Technische Wetenschappen
    Inventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
  • Patent number: 8741593
    Abstract: This invention has as its object a method for releasing a product by subjecting a compound of Formula (II?): R?7R?8(HX)C1-C2(YH)R?9R?10 to a chemical oxidation that cleaves the bond C1-C2 to obtain the product. In the compound of Formula (II?): R?7 to R?10, which are identical or different, correspond to a hydrogen atom, a substituted or unsubstituted alkyl group, or a substituted or unsubstituted functional group; X and Y, which are identical or different, are an oxygen atom, a sulfur atom, or an amine of Formula —NR11R12, wherein R11 is a hydrogen atom, an alkyl group, or a substituted or unsubstituted aryl group, and R12 is not a hydrogen atom. The invention also has as its object a method for releasing a product that comprises, before the chemical oxidation stage, a first step for preparing the compound of Formula (II?). The released product can be a volatile molecule or an active substance or else a specific product.
    Type: Grant
    Filed: November 20, 2006
    Date of Patent: June 3, 2014
    Assignee: Proteus
    Inventors: Jean-Louis Reymond, Denis Wahler, Frabrizzio Badalassi, Hong-Khanh Nguyen
  • Patent number: 8715697
    Abstract: The invention relates to the field of combating leishmaniases. Said invention results from the isolation, from wild isolates of Leishmania major, of a protein-coding gene known as LmPDI which has two regions that are identical to the sequence (Cys-Gly-His-Cys) of the potential active site of the protein disulphide isomerase (PDI). The LmPDI protein is predominantly expressed in the most virulent isolates of the parasite. Said protein forms a novel therapeutic target for developing anti-leishmaniasis medicaments and a novel element that can be used in the composition of immunogenic, and possibly vaccinating, preparations which are intended to protect a human or animal host against Leishmania.
    Type: Grant
    Filed: March 27, 2008
    Date of Patent: May 6, 2014
    Assignees: Institut Pasteur de Tunis, Institut Pasteur
    Inventors: Yosser Ben Achour, Mehdi Chenik, Hechmi Louzir, Koussay Dellagi
  • Patent number: 8716031
    Abstract: Disclosed is a convenient and effective means for quantifying an antigen-specific canine or human IgE.
    Type: Grant
    Filed: May 12, 2009
    Date of Patent: May 6, 2014
    Assignees: RIKEN, Animal Allergy Clinical Laboratories
    Inventors: Kenichi Masuda, Yasuyuki Ishii, Nobutaka Yasuda
  • Patent number: 8697368
    Abstract: Disclosed is a diagnostic marker for lung cancer comprising Hp?R as an active ingredient. Because blood, which is relatively easy to sample, is employed as a specimen, the diagnostic kit and marker for lung cancer is very simple and does not subject patients to a load compared to conventional methods that are directed to a biopsy. In addition, the kit and marker of the present invention is useful in the early diagnosis of lung cancer thanks to its high diagnostic sensitivity and selectivity.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: April 15, 2014
    Assignee: Paichai University-Academic Cooperation Foundation
    Inventor: Il Han Kim
  • Patent number: 8685662
    Abstract: The present invention pertains to a method for in vitro prognosticating and/or diagnosing cerebral cerebral malaria, wherein said method comprises a step of detecting non-erythroid spectrin or fragments thereof, and/or antibodies directed against non-erythroid spectrin, in a biological sample. Reagents and kits for performing this method are also disclosed.
    Type: Grant
    Filed: March 30, 2007
    Date of Patent: April 1, 2014
    Assignees: Institut Pasteur, Universite Pierre et Marie Curie (Paris 6)
    Inventors: Sylviane Pied, Vincent Guiyedi, Pierre-André Cazenave, Maryvonne Kombila, Youri Chanseaud
  • Patent number: 8663604
    Abstract: A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.
    Type: Grant
    Filed: September 4, 2008
    Date of Patent: March 4, 2014
    Assignee: The Regents of the University of California
    Inventors: Quoc-Thang Nguyen, David Kleinfeld, Lee F. Schroeder
  • Patent number: 8663939
    Abstract: The present invention provides a method of diagnosing a cyathostomin infection, said method comprising the step of identifying a level of anti-cyathostomin larval antigen antibodies in a sample, wherein a level of anti-cyathostomin larval antigen antibodies is indicative of a cyathostomin infection.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: March 4, 2014
    Assignee: Moredun Research Institute
    Inventors: Jacqui Matthews, Jane Hodgkinson, Christopher Proudman
  • Patent number: 8663910
    Abstract: A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.
    Type: Grant
    Filed: September 18, 2009
    Date of Patent: March 4, 2014
    Assignee: FUJIFILM Corporation
    Inventors: Mikinaga Mori, Junichi Katada, Hiroyuki Chiku, Takayoshi Oyamada
  • Patent number: 8642283
    Abstract: The present invention provides an assay for the identification of agents which can modulate TOR-mediated phosphorylation of substrate proteins. The assays of the invention utilize substrate proteins whose amino acid sequence contains the Ser/Thr motif recognized by TOR. Naturally occurring TOR which may be used in the methods of the invention include TOR isolated from a variety of species, particularly mammalian tissues.
    Type: Grant
    Filed: May 26, 2006
    Date of Patent: February 4, 2014
    Assignee: Sanford-Burnham Medical Research Institute
    Inventor: Robert T. Abraham
  • Patent number: 8637464
    Abstract: The present invention provides a method to identify a test compounds capability to modulate p75.sup.NTR induced apoptosis, said method comprising: i. Transfecting a suspension of eukaryotic cells with a vector encoding SEQ ID No.4 OR SEQ ID No.6), ii. Contacting said cells with the compound to be tested, and iii. Determine the apoptotic response in said cells, wherein an alteration in apoptotic response in the presence of said test compound compared to the apoptotic response in the absence of the test compound is an indication of the ability of the test compound to modulate p75.sup.NTR induced apoptosis.
    Type: Grant
    Filed: July 11, 2005
    Date of Patent: January 28, 2014
    Assignee: Janssen Pharmaceutica N.V.
    Inventors: Wouter David Bruinzeel, Miroslav Cik
  • Publication number: 20140017714
    Abstract: The present invention describes the method for determining the risk of future major adverse cardiovascular events, which comprises detection proteolytic fragments of IGFBP-4 or IGFBP-5 (insulin-like growth factor binding protein 4 or insulin-like growth factor binding protein 5) in patients' blood. The present invention provides antibodies and immunoas-says, suitable for specific measurement of proteolytic fragments of IGFBPs. In current invention the IGFBP fragments are suggested to be utilized as blood biomarkers for the risk prediction of major adverse cardiovascular events (MACE).
    Type: Application
    Filed: April 13, 2012
    Publication date: January 16, 2014
    Applicant: HYTEST LTD.
    Inventors: Alexey G. Katrukha, Alexander B. Postnikov, Tatiana I. Smolyanova, Alexey V. Kharitonov, Natalia N. Tamm
  • Publication number: 20140010825
    Abstract: Provided herein are compositions comprising purified antibodies and fragments thereof that are specifically immunoreactive to only human leukocyte antigen E (HLA-E) but not to other HLA Ia and HLA-Ib alleles. Also provided are methods of their making and diagnostic and therapeutic applications. The monospecific HLA-E antibodies are highly specific and can be used for diagnosing or localizing the presence of HLA-E on normal or diseased cells or tissues. The monospecific HLA-E antibodies can also be used for cancer therapies, likely through regulation of the CD94/NKG2a on Cytotoxic and/or Natural Killer T cells.
    Type: Application
    Filed: July 5, 2012
    Publication date: January 9, 2014
    Applicant: The Terasaki Family Foundation
    Inventors: Mepur H. Ravindranath, Paul I. Terasaki
  • Publication number: 20130336894
    Abstract: The present invention relates to antibodies against Oncofetal Antigen/immature Laminin receptor protein (OFA/iLRP) that can be used singly or in conjunction to detect or treat OFA/iLRP-related diseases. More specifically, the antibodies can be used for several purposes including: (i) detecting and measuring OFA/iLRP in different biofluids; and (ii) using OFA/iLRP with an antibody directed against the monomeric form and its associated diseases.
    Type: Application
    Filed: July 22, 2013
    Publication date: December 19, 2013
    Inventor: Eric W. Olle
  • Patent number: 8609356
    Abstract: The invention relates to an in vitro method for detecting pulmonary arterial hypertension (PAHT), or the risk of developing PAHT, which includes determining the presence and/or amount of anti-tenascin C antibodies in a biological sample from a patient.
    Type: Grant
    Filed: April 10, 2009
    Date of Patent: December 17, 2013
    Assignees: Assistance Publique-Hopitaux de Paris, Universite Paris Descartes, Universite Paris-Sud 11
    Inventors: Luc Mouthon, Marc Humbert, Mathieu Tamby
  • Publication number: 20130331329
    Abstract: The application relates to markers for seizures and epilepsy. Polypeptide expression panels or arrays are provided, comprising one or more probes capable of binding specific polypeptides in blood plasma or blood serum of a mammalian subject. Also provided are methods for detecting seizure, methods for predicting seizure, use of sICAM-5 in the treatment of seizure, methods for assessing the effectiveness of a treatment of seizure, and diagnostic kits.
    Type: Application
    Filed: February 24, 2012
    Publication date: December 12, 2013
    Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: John Robert Pollard, Peter B. Crino
  • Patent number: 8603765
    Abstract: The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and efficiently.
    Type: Grant
    Filed: October 27, 2009
    Date of Patent: December 10, 2013
    Assignee: Biocare Medical, LLC.
    Inventor: David Tacha
  • Patent number: 8592223
    Abstract: The invention provides, in certain embodiments, a method of assaying an indicator of, and treating, renal injury or renal disease. The method entails assaying a urine sample for periostin, wherein the presence of periostin at an elevated level indicates the presence and/or degree of renal injury or renal disease. The method also involves prescribing, initiating, or altering prophylaxis or therapy for renal injury or renal disease. Also provided, are methods of determining progression of these conditions, as well as methods of determining subjects' response to treatment.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: November 26, 2013
    Assignee: Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center
    Inventors: Sharon Adler, Bancha Satirapoj, Ying Wang, Janine LaPage, Cynthia C. Nast