Animal Tissue Cell Culture Patents (Class 435/70.3)
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Publication number: 20130190480Abstract: It is an object of the present invention to provide an oriented collagen/apatite material wherein an orientation is controlled, and a method of the oriented collagen/apatite material wherein an orientation is controlled. A method of producing an orientated collagen/apatite material according to the present invention, is characterized in that the method comprises; preparing a collagen having an orientation, seeding an osteoblast or a mesenchymal stem cell to produce and fix an apatite having an orientation similar to or almost the same as a direction of an orientation of the collagen on a surface and/or inside of the collagen. Furthermore, in a preferred embodiment of above mentioned method of producing an orientated collagen/apatite material according to the present invention, is characterized in that the osteoblast is an osteoblast like cell or an osteoblast obtained from a living organism.Type: ApplicationFiled: September 15, 2011Publication date: July 25, 2013Applicant: ATREE, INC.Inventors: Takayoshi Nakano, Aira Matsugaki, Takuya Ishimoto, Taro Saku, Yoshihiro Isobe
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Publication number: 20130183714Abstract: The present invention relates to a method for culturing mammalian cells in a culture medium which is transferrin free and which contains no lipophilic or synthetic nitrogen-containing chelators. Also provided is the use of the medium and a process for providing a mammalian product by culturing cells capable of producing the product in the medium.Type: ApplicationFiled: December 20, 2012Publication date: July 18, 2013Applicant: Medimmune LimitedInventors: Matthew David Osborne, Jonathan H. Dempsey
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Patent number: 8481290Abstract: An antibody specific for a chondroitin sulphate epitope is described, as is a hybridoma cell line which produces such an antibody. The antibody is useful in the diagnosis and treatment of connective tissue diseases, such as arthritis and sarcomas. Test kits and pharmaceutical compositions are also described.Type: GrantFiled: June 1, 2005Date of Patent: July 9, 2013Assignees: The National Research Council of Thailand, The Thailand Research Fund, Chiang Mai University of ThailandInventors: Prachya Kongtawelert, Tim Hardingham, Siriwan Ong-Chai, Kazuyuki Sugahara, Peraphan Pothacharoen, Nattachai Tiengburanathum
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Patent number: 8470562Abstract: A highly efficient method of making a primary cell derived biologic by purifying mononuclear cells (MNCs) in a automated cell processor to remove contaminating cells by loading leukocytes onto lymphocyte separation medium (LSM) and centrifuging the medium to obtain purified MNCs, storing the MNCs overnight in a closed sterile bag system, stimulating an induction mixture of the MNCs with phytohemagglutinin (PHA) or other mitogen and ciprofloxacin in a scalable cell culture device and producing a primary cell derived biologic from the MNCs, removing the mitogen from the induction mixture by filtering, incubating the induction mixture, clarifying the induction mixture by filtering to obtain a primary cell derived biologic supernatant, and clearing the primary cell derived biologic supernatant from adventitious agents by anion exchange chromatography, filtration. A closed system prevents contamination of the resulting primary cell derived biologic. An automated method of purifying cells.Type: GrantFiled: April 14, 2009Date of Patent: June 25, 2013Assignee: IRX Therapeutics, Inc.Inventors: George J. Fennington, Jr., Harvey J. Brandwein
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Publication number: 20130095077Abstract: A somatic stem cell that is CD10+, CXCR4+, and CD31+ and another somatic stem cell that is CD 105+, CD44+, and nestin+. Also disclosed are both a method of preparing these stem cells and a method of using them to treat degenerative diseases, e.g., a muscle-degenerative disease. The invention further includes making and using liver cells derived from the somatic cell that is CD105+, CD44+, and nestin+.Type: ApplicationFiled: September 28, 2012Publication date: April 18, 2013Applicant: StemBios Technologies, Inc.Inventor: James Wang
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Patent number: 8361751Abstract: A cell in which the activity of a protein relating to transport of an intracellular sugar nucleotide, GDP-fucose, to the Golgi body is more decreased or deleted than its parent cell; a process for producing an antibody composition using the cell; a transgenic non-human animal or plant or the progenies thereof, in which genome is modified so as to have a decreased or deleted activity of a protein relating to transport of an intracellular sugar nucleotide, GDP-fucose, to the Golgi body; a process for producing an antibody composition from the animal or plant; and a medicament comprising the antibody composition.Type: GrantFiled: May 19, 2010Date of Patent: January 29, 2013Assignee: Kyowa Hakko Kirin Co., LtdInventors: Yutaka Kanda, Mitsuo Satoh, Katsuhiro Mori
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Patent number: 8354105Abstract: The present invention relates to methods of modulating (e.g., reducing) the mannose content, particularly high-mannose content of recombinant glycoproteins.Type: GrantFiled: September 29, 2009Date of Patent: January 15, 2013Assignee: Amgen Inc.Inventors: Jian Wu, Nicole Le, Michael De La Cruz, Gregory Flynn
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Publication number: 20130012446Abstract: Methods of manufacturing collagen and other extracellular matrix components from SCCs are disclosed. The extracellular matrix components are useful in cosmetic applications, and can be manufactured free of immunogenic concerns and contaminants while controlling for other factors that commonly impact product quality and usefulness.Type: ApplicationFiled: March 18, 2011Publication date: January 10, 2013Applicant: AMERSTEM, INCInventors: M. Rocio Sierra-Honigmann, Jaime Flores-Riveros, Martin Sierra
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Patent number: 8343740Abstract: A method for fabricating a micro-organ device comprises providing a microscale support having one or more microfluidic channels and one or more micro-chambers for housing a micro-organ and printing a micro-organ on the microscale support using a cell suspension in a syringe controlled by a computer-aided tissue engineering system, wherein the cell suspension comprises cells suspended in a solution containing a material that functions as a three-dimensional scaffold. The printing is performed with the computer-aided tissue engineering system according to a particular pattern. The micro-organ device comprises at least one micro-chamber each housing a micro-organ; and at least one microfluidic channel connected to the micro-chamber, wherein the micro-organ comprises cells arranged in a configuration that includes microscale spacing between portions of the cells to facilitate diffusion exchange between the cells and a medium supplied from the at least one microfluidic channel.Type: GrantFiled: March 28, 2008Date of Patent: January 1, 2013Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space AdministrationInventors: Steve R. Gonda, Iris von Gustedt-Gonda, legal representative, Robert C. Chang, Binil Starly, Christopher Culbertson, Heidi L. Holtorf, Wei Sun, Julia Leslie
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Patent number: 8338376Abstract: This invention relates to methods of treating disease with soluble inhibitors of the lymphotoxin pathway having improved properties. This invention also relates to improved LTBR-Ig fusion proteins, and pharmaceutical compositions thereof.Type: GrantFiled: September 15, 2009Date of Patent: December 25, 2012Assignee: Biogen Idec MA Inc.Inventors: Evan Beckman, Graham K. Farrington, Werner Meier, Jeffrey L. Browning
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Publication number: 20120316320Abstract: The presently disclosed subject matter relates to Managed Ecosystem Fermentation (MEF) which is a continuous microbial process utilizing a managed ecosystem approach employing dozens to thousands of species of microorganisms, occurring in a controlled artificial environment and consuming organic materials without benefit of sterilization. The process of utilizing this fermentation for the consumption of organic materials on a continuous basis is within the scope of this disclosed subject matter. The process of separating chemicals as industrial chemicals from this fermentation on a continuous basis is within the scope of this disclosed subject matter. The process of separating biomass useful as high protein animal feed or fertilizer from this fermentation on a continuous (or semi-continuous) basis is within the scope of this disclosed subject matter.Type: ApplicationFiled: June 11, 2012Publication date: December 13, 2012Inventors: Edward Arthur Calt, JR., Herbert Graham Tull, IV, Stanley Sylvester Toporek
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Patent number: 8323928Abstract: Anti-lentivirus vaccines and immunotherapeutics and methods for preparing and using same are disclosed. The vaccines and immunotherapeutics are produced using non-immunosuppressive lentivirus trans-activator of transcription (Tat) proteins. An associated in vitro ultra-sensitive macrophage Tat bioassay is disclosed for assessing the immunosuppressive qualities of the lentivirus Tat preparations of the present invention. Additionally, a related long-term T4 cell propagation system for characterizing lentivirus Tat is also disclosed. The present invention has additional utility in the treatment and prevention of AIDS.Type: GrantFiled: March 24, 2006Date of Patent: December 4, 2012Assignee: PIN Pharma, Inc.Inventor: David I. Cohen
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Publication number: 20120301894Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.Type: ApplicationFiled: February 17, 2012Publication date: November 29, 2012Applicant: Life Technologies CorporationInventors: Brian AGNEW, Kyle R. Gee, Tamara G. Nyberg
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Publication number: 20120277165Abstract: The present invention provides methods and materials useful for monitoring and regulating the glycosylation of glycoproteins that are recombinantly produced from cells. In particular, methods are provided for monitoring and regulating levels of cellular indicators which affect the level of fucosylation produced by cells.Type: ApplicationFiled: June 4, 2010Publication date: November 1, 2012Inventors: Brian E. Collins, Lakshmanan Thiruneelakantapillai, Dorota A. Bulik, Kevin Millea
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Patent number: 8298790Abstract: Disclosed is a method for coexpressing IL-12 (interleukin-12) and IL-23 (interleukin-23), which comprises the steps of: (a) preparing vectors comprising monocistronic expression constructs of each of nucleotide sequences encoding the p35 subunit, the p40 subunit and the p19 subunit, or preparing a vector comprising a polycistronic expression construct of nucleotide sequences encoding the p35 subunit, the p40 subunit and the p19 subunit; (b) transforming the expression constructs into a host cell; and (c) culturing the transformed host cell to obtain IL-12 and IL-23, a vector for coexpressing IL-12 and IL-23, and a pharmaceutical anti-tumor composition comprising the vectors.Type: GrantFiled: January 17, 2008Date of Patent: October 30, 2012Inventor: Chae-Ok Yun
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Publication number: 20120263728Abstract: The present invention provides a method for generating a functionally modifying antibody to an ion channel comprising immunizing a host with a cyclic peptide comprising at least part of an extracellular sequence of said ion channel.Type: ApplicationFiled: June 14, 2012Publication date: October 18, 2012Applicant: UCB Pharma S.A.Inventor: Terence Seward Baker
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Publication number: 20120263727Abstract: The present invention provides a method for generating a functionally modifying antibody to an ion channel comprising immunizing a host with a cyclic peptide comprising at least part of an extracellular sequence of said ion channel.Type: ApplicationFiled: October 27, 2010Publication date: October 18, 2012Applicant: UCB PHARMA S.A.Inventor: Terence Seward Baker
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Publication number: 20120264170Abstract: The present invention provides, inter alia, an isolated cell line, 3M as well as methods for making such a cell line and methods of using such a cell line, e.g., to produce a protein such as an immunoglobulin.Type: ApplicationFiled: December 16, 2010Publication date: October 18, 2012Inventors: Ankit A. Merchant, Yung-Shyeng Tsao
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Publication number: 20120264916Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.Type: ApplicationFiled: October 1, 2010Publication date: October 18, 2012Applicant: Biogen Idec MA Inc.Inventors: David Evans, R. Blake Pepinsky, Dingyi Wen, Rashmi Rohit Kshirsagar, Karin Lucas
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Publication number: 20120258082Abstract: In the present invention, CD8+ conventional dendritic cells (CD8+ cDCs) and equivalents thereof (eCD8+ cDCs) in mouse and human have been established as major source of IFN-lambda (IFN-?) in response to double-stranded (ds) nucleic acids. The invention relates to therapeutic applications of ds nucleic acids or analogs thereof targeting CD8+ and/or eCD8+ cDCs in the prevention and/or treatment of infectious diseases, preferably viral infections, or cancer. Furthermore, the invention relates to an in vitro method for producing IFN-? and/or generating or obtaining a population of IFN-? producing CD8+ or eCD8+ cDCs as well as in vitro method for detecting or screening for CD8+ and/or eCD8+ cDCs. In addition, the invention relates to a Flt3-ligand or a M-CSF receptor ligand for use in increasing the level of CD8+ and/or eCD8+ cDCs in a subject suffering from an infectious disease or cancer.Type: ApplicationFiled: December 17, 2010Publication date: October 11, 2012Applicant: Bavarian Nordic A/SInventor: Hubertus Hochrein
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Patent number: 8278068Abstract: The present invention relates to the use of an ex vivo human skin sample as a biological model, particularly for the assessment of pharmacological and cosmetic effects.Type: GrantFiled: June 19, 2008Date of Patent: October 2, 2012Assignees: Symrise AG, Cutech SRLInventors: Gabriele Vielhaber, Paolo Pertile
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Patent number: 8273553Abstract: The invention relates to a process for the manufacturing of a protein, such as growth hormone, in mammalian cells cultured in a serum-free medium (medium free of components derived from animal serum). The medium contains cobalt and, optionally, cadmium or cyclodextrin.Type: GrantFiled: April 16, 2010Date of Patent: September 25, 2012Assignee: Ares Trading S.A.Inventors: Jose Casatorres Hernandez, Carlos Martin Piera
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Publication number: 20120231504Abstract: A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation.Type: ApplicationFiled: September 3, 2011Publication date: September 13, 2012Applicant: Therapeutic Proteins Inc.Inventor: Sarfaraz K. Niazi
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Publication number: 20120225445Abstract: Adipose cells (sebocytes) are described, The invention especially relates to sebaceous gland cells and to a sebaceous gland cell line with the property of being continuously grown over many sub-cultures. The sebocytes are excellently suited for useful applications.Type: ApplicationFiled: September 29, 2011Publication date: September 6, 2012Inventor: Christos Zouboulis
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Patent number: 8257947Abstract: The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible three-dimensional surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.Type: GrantFiled: January 30, 2009Date of Patent: September 4, 2012Inventors: Gail K. Naughton, Frank Ziegler, Mark Baumgartner, Kyle Nickey
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Publication number: 20120214204Abstract: The invention relates to cell culture media optimized for exposure to ultraviolet C (UVC) light exposure and related methods.Type: ApplicationFiled: February 17, 2012Publication date: August 23, 2012Applicant: AMGEN INC.Inventors: Roger HART, Robert Michael Boychyn
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Patent number: 8232077Abstract: Ovarian germ-line-competent embryonic stem cells (GLC-ESC) are cultured, either in the presence or absence of a compound having estrogenic activity. The GLC-ESC are either collected prior to specific commitment or are permitted to remain in the culture medium for a time sufficient to develop into oocytes, and the oocytes may be fertilized by adding sperm to the culture medium. The fertilized oocytes may be permitted to develop into embryos, which may be transferred into the uterus of an adult human female or frozen for later use. The invention provides a method for obtaining by in vitro fertilization an embryo that is genetically related to a human female who is not producing oocytes.Type: GrantFiled: June 23, 2006Date of Patent: July 31, 2012Assignee: Ovacyte LLCInventors: Antonin Bukovsky, Michael R. Caudle
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Patent number: 8232100Abstract: Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified.Type: GrantFiled: July 21, 2010Date of Patent: July 31, 2012Assignee: Isis Innovation LimitedInventors: Herman Waldmann, Paul J. Fairchild, Richard Gardner, Frances Brook
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Patent number: 8221998Abstract: This present invention relates to the use of the B1 domain of Protein G as an epitope tag for over-expression of proteins in mammalian cells.Type: GrantFiled: May 19, 2006Date of Patent: July 17, 2012Assignee: GlaxoSmithKline LLCInventors: Kyung Oh Johanson, John J. Trill
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Publication number: 20120171724Abstract: A system and method of adapting host cells to suspension cell culture and a suspension cell line produced thereby are disclosed. The method includes the serial replating of substantially undiluted culture cells onto a surface area until cell clumps are visualized and then, upon cell clumping, moving the cells into a suspension culture system.Type: ApplicationFiled: January 5, 2012Publication date: July 5, 2012Inventors: Gabriela D.C. Denning, Richard E. Gautney
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Publication number: 20120171171Abstract: Aspects of the present invention include methods and compositions related to the production, identification and use of embryonic progenitor cell lines that are capable of undergoing chondrogenesis. A number of exemplary chondrogenic cell lines derived from primordial stem cells are disclosed. The chondrogenic cell lines described herein are robust, can expand for >40 passages, and have site-specific purity, thus providing for compositions and methods of producing diverse cartilage types with unique molecular compositions for use in research and therapy.Type: ApplicationFiled: July 16, 2010Publication date: July 5, 2012Inventors: Michael D. West, Hal Sternberg, Karen B. Chapman
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Publication number: 20120149063Abstract: The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.Type: ApplicationFiled: February 13, 2012Publication date: June 14, 2012Applicant: DSM IP ASSETS B.V.Inventors: Gerben Meile ZIJLSTRA, Robert Patrick HOF, Jacobs SCHILDER
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Publication number: 20120141399Abstract: The present invention relates to a culture medium for the fetus-derived mesenchymal stem cells in amniotic fluid. More particularly, the present invention relates to a composition for improving skin conditions, comprising the culture medium of fetus-derived mesenchymal stem cells in amniotic fluid as an active ingredient, in which the skin conditions to be improved include whitening, wrinkles, skin damages caused by UV rays or skin lifting. Further, the present invention relates to a method for preparing the composition, comprising the steps of culturing the fetus-derived mesenchymal stem cells in amniotic fluid; and collecting the culture medium.Type: ApplicationFiled: March 19, 2010Publication date: June 7, 2012Applicant: STEMMEDIENCE CO., LTDInventors: Seungkwon You, Byung Sun Yoon, Jai-Hee Moon, Eun Kyoung Jun, Jonggun Kim, Hye-Youn Jung, Jung Han Lee, Eulsoon Park, Isaac Maeng, Jun Sung Kim, Jang Ho Lee, Hwang Heui Lee, Jong Won Lee, Kyoung Shik Cho
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Publication number: 20120135003Abstract: An antibody binding to IPC was obtained by using an animal cell in which a cell membrane protein associatable with ILT7 was co-expressed as an immunogen. The antibody of the invention has a high specificity which allows immunological distinction between other ILT family molecules and ILT7. The anti-ILT7 antibody of the invention bound to IPC and inhibited the activity thereof. With the anti-ILT7 antibody of the invention, the IPC activity can be inhibited and an interferon-related disease can be treated or prevented. ILT7 expression is maintained even in IPC in the presence of IFN?. Therefore, an inhibitory action of IPC activity by the anti-ILT7 antibody can be expected even in an autoimmune disease patient with an increased production of IFN?.Type: ApplicationFiled: November 22, 2011Publication date: May 31, 2012Applicant: SBI Biotech, Ltd.Inventors: Yumiko Kamogawa, Minkwon Cho, Naoko Arai, Koji Ishida
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Publication number: 20120100144Abstract: A correlation between expression of JMJD6 polypeptide and breast cancer metastasis exists accordingly the present invention relates a diagnostic, prognostic and therapeutic biomarker to distinguish between early and advanced/metastatic cancer particularly breast cancer, including compounds and methods to treat the same.Type: ApplicationFiled: March 2, 2010Publication date: April 26, 2012Applicant: Agency for Science, technology and ResearchInventors: Kartik V. Desai, Edison T. Liu, Lance David Miller
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Patent number: 8148111Abstract: A carrier for cell culture is provided which improves the cell proliferativity in serum-free culture and which is free from risk from infection factor contamination. The gist of the features of the present invention is to be formed of a crosslinked poly(meth)acrylic acid (salt) particle (A) and an artificial polypeptide (P) having at least one cell-adhesive minimal amino acid sequence (X) in one molecule and to have a water retention value of from 2 to 50 g/g. The (A) is preferably a particle produced by reversed phase suspension polymerization of an aqueous monomer solution containing (meth)acrylic acid and/or an alkali metal salt of (meth)acrylic acid. The (P) preferably has at least one auxiliary amino acid sequence (Y) in one molecule of the (P). The (X) is preferably an Arg Gly Asp sequence.Type: GrantFiled: March 13, 2007Date of Patent: April 3, 2012Assignee: Sanyo Chemical Industries, Ltd.Inventors: Masato Kurokawa, Kazuhiro Takahashi
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Publication number: 20120077213Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.Type: ApplicationFiled: November 30, 2011Publication date: March 29, 2012Applicant: Abbott LaboratoriesInventors: Itzcoatl A. Pla, Joseph G. Matuck, John C. Fann, Christof Schulz, Nichole A. Roy, David F. Bruton, James McIntire, Yu-Hsiang David Chang, Thomas Seewoester
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Patent number: 8143022Abstract: The present invention relates to a method for producing infectious hepatitis C virus (HCV) particles, comprising a step of introducing an expression vector into a cell that allows HCV proliferation, such expression vector comprising: DNA sequences encoding the 5? untranslated region, structural proteins, and, if necessary, non-structural proteins of HCV and DNA sequences encoding non-structural proteins and the 3? untranslated region derived from the HCV JFH1 strain, which are located downstream of a polymerase I promoter; and a DNA fragment containing an RNA polymerase I terminator, which is located further downstream thereof.Type: GrantFiled: September 29, 2006Date of Patent: March 27, 2012Assignee: Tokyo Metropolitan Institute of Medical ScienceInventors: Jun-ichi Tanabe, Saburo Sone, Takaji Wakita, Koji Ishii, Ryosuke Suzuki, Tetsuro Suzuki, Tatsuo Miyamura
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Patent number: 8137934Abstract: The present invention provides a medicine, comprising (a) an Otx2 protein or its partial peptide, or a salt thereof, or (b) a DNA or an RNA encoding an Otx2 protein or its partial peptide. The present medicine is useful as an agent for preventing, treating or suppressing progression of a retinal disease including retinal degeneration. In addition, the present medicine is useful, for example, as an agent for inducing differentiation from a retinal stem cell into a retinal photoreceptor cell, in the transplantation of a cell into the retina of patients suffering from retinal diseases.Type: GrantFiled: April 26, 2010Date of Patent: March 20, 2012Assignee: Japan Science and Technology AgencyInventor: Takahisa Furukawa
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Publication number: 20120064163Abstract: Disclosed are mammalian tumor and/or cancer cell conditioned substrate preparations having tumor inhibiting activity. In some embodiments, the mammalian tumor and/or cancer cell conditioned substrate preparations are essentially free of cellular components. These preparations comprise a conditioned heterologous acellular collagenous tissue preparation, and may be prepared using a mammalian extracellular matrix substrate. The conditioned substrates include many different anti-tumor and/or anti-cancer biomolecules, such as that population of anti-tumor biomolecules that are secreted and/or produced by mammalian tumor and/or cancer cells as they grow on a substrate, thus imparting the anti-tumor and/or anti-cancer properties to the conditioned substrates of the invention. The present disclosure also provides methods for preparing the mammalian tumor and/or cancer cell conditioned substrates, as well as methods for using the preparations to inhibit tumor growth, such as in a vaccine or wound dressing.Type: ApplicationFiled: September 13, 2010Publication date: March 15, 2012Inventors: Mark A. Suckow, Michael C. Hiles
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Publication number: 20120040917Abstract: The present invention relates to compositions and methods for preparing splice variants of TNFalpha receptor (TNFR) in vivo or in vitro, and the resulting TNFR protein variants. Such variants may be prepared by controlling the splicing of pre-mRNA molecules and regulating protein expression with splice switching oligonucleotides or splice switching oligomers (SSOs). The preferred SSOs according to the invention target exon 7 or 8 of TNFR1 (TNFRSF1A) or TNFR2 (TNFRSF1A) pre-mRNA, typically resulting in the production of TNFR variants which comprise a deletion in part or the entire exon 7 or 8 respectfully. SSOs targeting exon 7 are found to result in a soluble form of the TNFR, which has therapeutic benefit for treatment of inflammatory diseases. The SSO's are characterized in that they are substantially incapable or incapable of recruiting RNaseH.Type: ApplicationFiled: December 3, 2010Publication date: February 16, 2012Inventors: HENRIK ØRUM, PETER L. SAZANI
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Publication number: 20120027858Abstract: The invention relates to composition and a method of using the composition for modulating proliferation, invasiveness, the expression of a biomarker of an abnormal cell, of reducing the risk of a patient cell becoming abnormal, or of modulating proliferation of a carcinoma-associated fibroblast or of a tumor-associated macrophage. The invention also relates to a method of culturing the composition to produce molecules that modulate abnormal cell proliferation, invasiveness, or metastasis. The composition comprises a biocompatible matrix and cells engrafted thereon.Type: ApplicationFiled: February 4, 2010Publication date: February 2, 2012Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGYInventors: Joseph W. Franses, Elazer R. Edelman, Angelo Manuel De Almeida Cardoso, Helen Marie Nugent
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Publication number: 20120021459Abstract: The invention provides stable feed media containing pyruvate and methods for stabilizing feed media by adding pyruvate. The invention further provides methods for producing proteins using such media and proteins produced through the use of such methods.Type: ApplicationFiled: September 26, 2011Publication date: January 26, 2012Applicant: Amgen Inc.Inventors: Arvia Eleanor Morris, Aurora Villegas Viaje, Erika Pineda
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Patent number: 8101388Abstract: The present invention in one embodiment provides a method for extracting molecular material including providing a probe comprising a penetration portion having a nanoscale surface for penetrating a biological compartment, a receptor present on the penetrating portion of the probe, wherein the receptor has an affinity for a target molecular material from the biological compartment; inserting the probe into the biological compartment, the receptor present on the penetrating portion of the probe engages the target molecular material; and extracting the probe and the target molecular material engaged to the inserting portion of the probe from the biological compartment.Type: GrantFiled: September 28, 2007Date of Patent: January 24, 2012Assignee: UT-Battelle, LLCInventors: Timothy E. McKnight, Anatoli V. Melechko, Michael L. Simpson
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Patent number: 8101167Abstract: The invention relates to compositions containing an agent that has an irritant side effect and a conditioned cell culture medium and/or of an extract thereof, for use, e.g., in the treatment of signs of inflammation and/or of immune disorders, the medium being obtainable by contact with at least one culture of digestive tract cells and at least one probiotic microorganisms. Methods of use.Type: GrantFiled: February 26, 2008Date of Patent: January 24, 2012Assignee: L'OrealInventor: Audrey Gueniche
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Publication number: 20120015402Abstract: An objective of the present invention is to facilitate the acquisition of antibody-producing cells that are infiltrating virus-infected cells, cancer cells, abnormal cells forming a benign hyperplasia, and the like, and to improve the efficiency of the production of antibodies as well as nucleic acids encoding them from the antibody-producing cells. The present inventors discovered that, when cancer tissues comprising infiltrating lymphocytes are transplanted into highly immunodeficient animals that do not have T cells, B cells, and NK cells and further exhibit a low IFN production ability, the differentiation and proliferation of infiltrating lymphocytes are unexpectedly promoted, and the number of plasma cells that produce antibodies recognizing cancer tissues increases dramatically, plasma cells can be separated easily, and antibodies or nucleic acids encoding them can be easily prepared from the plasma cells.Type: ApplicationFiled: July 21, 2011Publication date: January 19, 2012Applicants: CHUGAI SEIYAKU KABUSHIKI KAISHA, CIEA INTERNATIONAL INC., PHARMALOGICALS RESEARCH PTE. LTD.Inventors: Masayuki Tsuchiya, Masami Suzuki, Kenji Yoshida, Etsuko Fujii, Miho Watanabe, Koichi Matsubara, Yu Jau Chen, Juliana Sim
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Publication number: 20120009233Abstract: The invention is directed compositions containing growth agents synthesized from cultured cells from skin. Skin cells such as keratinocytes and dermal fibroblasts are cultured in vitro in cell medium and in the course of culture the cultured cells synthesize and secrete agents into the cell medium. The medium containing agents are collected and incorporated into pharmaceutical or cosmetic preparations to treat an individual. The preparation is applied and has a rejuvenating effect on the cells and tissue.Type: ApplicationFiled: June 7, 2011Publication date: January 12, 2012Applicant: Organogenesis, Inc.Inventor: Andrew J. Nixon
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Publication number: 20110318357Abstract: The invention is based on the discovery of the epitope in the Stx2 protein for the 11 E1O antibody. The invention features compositions containing non-full length Stx2 polypeptides that include the 11 E1O monoclonal antibody epitope. The invention also features methods of producing anti-Stx2 antibodies specific for the 11 E1O epitope of the Stx2 protein. Additionally, the invention features methods for treating a subject having, or at risk of developing, a Shiga toxin associated disease (e.g., hemolytic uremia syndrome and diseases associated with E. coli and S. dysenteriae infection) with a polypeptide that includes the 11 E1O epitope or with an anti-Stx2 antibody developed using the methods of the invention. Furthermore, the invention features the detection of Stx2 in a sample using the antibodies developed using the methods of the invention.Type: ApplicationFiled: January 21, 2010Publication date: December 29, 2011Applicant: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.Inventors: Alison O'Brien, Angela Melton-Celsa, Michael Smith, James Sinclair
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Publication number: 20110300580Abstract: Described is a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein the DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG start codon or a TTG start codon, and wherein the ORF sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native ORF sequence that encodes the selectable marker protein. Further provided are such DNA molecules wherein the ORF sequence that encodes a selectable marker polypeptide is part of a multicistronic transcription unit that further comprises an open reading frame sequence encoding a polypeptide of interest. Also described are methods for obtaining host cells expressing a polypeptide of interest, wherein the host cells comprise the DNA molecules described herein.Type: ApplicationFiled: July 18, 2011Publication date: December 8, 2011Inventors: Arie Pieter Otte, Henricus Johannes Maria Van Blokland, Theodorus Hendrikus Jacobus Kwaks, Richard George Antonius Bernardus Sewalt
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Patent number: 8071745Abstract: Disclosed are methods and compositions for early diagnosis, monitoring and treatment of retinal dystrophy, age-related macular degeneration, Bardet-Biedel syndrome, Bassen-kornzweig syndrome, best disease, choroidema, gyrate atrophy, congenital amourosis, refsun syndrome, stargardt disease and Usher syndrome. In particular, the invention relates to a protein, termed “Rdcvf1,” that is differentially transcribed and expressed in subjects suffering from retinal dystrophies and the like, such as retinal dystrophy and age-related macular degeneration compared with non-sufferers, antibodies which recognize this protein, and methods for diagnosing such conditions.Type: GrantFiled: April 25, 2007Date of Patent: December 6, 2011Assignees: Novartis AG, Universite de StrasbourgInventors: Thierry Léveillard, José Alain Sahel, Saddek Mohand-Said, David Hicks