Incubators Or Racks Or Holders For Culture Plates Or Containers Patents (Class 435/809)
  • Patent number: 8986982
    Abstract: A thermal cycle system and method suitable for mass production of DNA comprising a temperature control body having at least two sectors. Each sector has at least one heater, cooler, or other means for changing temperature. A path traverses the sectors in a cyclical fashion. In use, a piece of tubing or other means for conveying is placed along the path and a reaction mixture is pumped or otherwise moved along the path such that the reaction mixture is repetitively heated or cooled to varying temperatures as the reaction mixture cyclically traverses the sectors. The reaction mixture thereby reacts to form a product. In particular, polymerase chain reaction reactants may continuously be pumped through the tubing to amplify DNA. The temperature control body is preferably a single aluminum cylinder with a grooved channel circling around its exterior surface, and preferably has wedge-shaped or pie-shaped sectors separated by a thermal barrier.
    Type: Grant
    Filed: September 19, 2012
    Date of Patent: March 24, 2015
    Assignee: Marshall University Research Corporation
    Inventors: Derek A. Gregg, Elizabeth E. Murray, Michael L. Norton, Justin T. Swick, Herbert Tesser
  • Patent number: 8945486
    Abstract: A microwell device is provided. The device includes a plate having a upper surface. The upper surface has first and second recesses formed therein. Each recess has an outer periphery. First and second portions of microwells are formed in upper surface of the plate. The first portion of microwells are spaced about the outer periphery of the first recess and the second portion of microwells spaced about the outer periphery of the first recess. A first barrier is about a first portions of the microwells for fluidicly isolating the first portion of the microwells and a second barrier about a second portions of microwells for fluidicly isolating the second portion of the microwells.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: February 3, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jay W. Warrick, John Yin, Stephen M. Lindsay
  • Patent number: 8920725
    Abstract: The present invention is an improved apparatus for sample analysis. The apparatus employs an assay component containing a membrane having one or a plurality of analyte-specific binding agents attached thereto, a means for absorbing liquid, and a piston means for drawing analytes through said membrane into said means for absorbing liquid. The apparatus is configured to be portable and provide a detector for detecting binding of an analyte to an analyte-specific binding agent, a plurality of data acquisition components, and a computer for integrating, analyzing and storing the detected analyte specific binding and acquired data.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: December 30, 2014
    Assignee: Montecito Bio-Sciences, Ltd.
    Inventors: Edward W. Withrow, III, Jorn Gorlach
  • Patent number: 8859263
    Abstract: The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
    Type: Grant
    Filed: August 7, 2012
    Date of Patent: October 14, 2014
    Assignee: University of Pittsburgh
    Inventors: Joel S. Greenberger, Paul A. DiMilla, Michael M. Domach, Raymond K. Houck
  • Patent number: 8845985
    Abstract: A rack for a plurality of capped tubes, including a frame having a longitudinally extending bottom base member having a row of spaced open top recesses adapted to receive the capped tubes. Vertical supports at the ends of the row of recesses fix a cover member above the base member. The cover member has openings therethrough smaller than the capped tubes. A wall is along one longitudinal side between the base member and the cover member, with the other longitudinal side between the base member and the cover member being open. The base member and cover member are vertically spaced a distance sufficient to permit tipped capped tubes to be moved into the frame with the tipped tubes then dropped into the recesses and tipped back to an upright vertical position.
    Type: Grant
    Filed: August 10, 2012
    Date of Patent: September 30, 2014
    Assignee: Abbott Laboratories
    Inventors: Stephen L. Herchenbach, E. Kim Pavlovic
  • Patent number: 8822210
    Abstract: An incubator (1) has an incubation chamber (2) and a shaking device (4) to shake receptacles (5) placed in the incubation chamber (2). A device chamber (3) adjoins the incubation chamber (2) and accommodates at least parts of the shaking device (4). The shaking device (4) has a base plate (8) that seals off the incubation chamber (2) from the device chamber 3. A shaking table (11) is on the inner face (9) of the base plate (8) and can be moved in a horizontal plane by a drive arm (10). A motor (34) is arranged on the outer face (37) of the base plate (8). The motor (34) drives a drive shaft (14) that is rotatably mounted in the base plate (8) and that is operatively connected to the drive arm (10).
    Type: Grant
    Filed: February 17, 2009
    Date of Patent: September 2, 2014
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Reinhard Baumfalk, Stefan Obermann, Rainer Sandrock
  • Patent number: 8795151
    Abstract: An infant care system and apparatus includes a horizontal surface to support an infant. A microenvironment region is defined around the horizontal surface by at least one wall. A graphical display is disposed within the microenvironment region. A diagnostic imaging device is at least partially disposed within the microenvironment region to obtain diagnostic images of an infant disposed within the microenvironment region.
    Type: Grant
    Filed: November 8, 2010
    Date of Patent: August 5, 2014
    Assignee: General Electric Company
    Inventors: Steven Mitchell Falk, David M. Mills, Lawrence G. Ten Eyck, Marjorie McCue
  • Patent number: 8796010
    Abstract: An isolator for cultivating cells including a working chamber having a plurality of gloves arranged side by side into which operator's hands are inserted to operate cells, the working chamber being sectioned into at least an operation area for operating the cells, and an auxiliary working area for opening a packaged auxiliary instrument used to operate the cells, a gas supply unit that supplies gas into the working chamber so that the gas flows downwardly from an upper side in the working chamber, and a gas flow control unit for controlling the flow of the downwardly flowing gas so that the gas flows from the operation area to the auxiliary working area around the gloves, wherein the gas flow control unit has an exhaust hole portion that has an opened area for passing the gas therethrough and is provided at a lower portion of at least the auxiliary working area, and through which the gas in the auxiliary working area is exhausted.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: August 5, 2014
    Assignees: Panasonic Healthcare Co., Ltd., Shibuya Kogyo Co., Ltd.
    Inventors: Yasuhiko Yokoi, Akifumi Iwama, Shinji Fukui, Atsushi Nakao, Hiroshi Yamamoto, Jirou Ohnishi, Hironobu Sunayama, Kanjun Yamamoto
  • Patent number: 8765077
    Abstract: Multiplex binding assay assemblies are disclosed. The assemblies generally include at least one assay bar that includes a top side, a bottom side, and at least one well accessible from the top side of the assay bar. Each well includes a side surface, a bottom surface, an open top end, and at least one secondary container, with each secondary container including a capillary tube that (i) begins at a location within an interior volume of the well and (ii) ends at a location beneath the bottom surface of the assay bar. The assemblies further include a dispenser bar that is adapted to be positioned adjacent to the top side of the assay bar, which includes one or more reservoirs that are configured to provide one or more reagents to the at least one secondary container located in each well of the assay bar.
    Type: Grant
    Filed: August 2, 2011
    Date of Patent: July 1, 2014
    Inventor: Pierre F. Indermuhle
  • Patent number: 8709364
    Abstract: The present invention provides a heat retaining device for solving problematic adhesion of a container to the heat retaining device. The heat retaining device includes a supporting rod, a top plate, a bottom plate, a heat retaining body, and a supporting spring. A pressing force applied to the container contained in the hole of the heat retaining body compresses the supporting spring to move the heat retaining body downward along the supporting rod. Release of the pressing force moves the heat retaining body upward by an elastic force of the supporting spring until the heat retaining body comes into contact with the top plate.
    Type: Grant
    Filed: October 20, 2010
    Date of Patent: April 29, 2014
    Assignee: Hitachi-High-Technologies Corporation
    Inventors: Akimasa Osaka, Minoru Sano, Hideo Kanno, Akiyuki Nemoto, Yoshiyuki Shoji
  • Patent number: 8697005
    Abstract: Multiplex binding assay assemblies are disclosed. The assemblies include at least one assay bar that has a top side, a bottom side, and at least one well accessible from the top side of the assay bar, with each well including a side surface, a bottom surface, an open top end. Each well also includes at least one secondary container, with each secondary container including a capillary tube that (i) begins at a location within an interior volume of the well and (ii) ends at a location beneath the bottom side of the assay bar. The assemblies further include a guiding track, which includes a set of two rails, with each rail having its own separate groove. Such grooves are configured to run parallel to each other with a distance between such grooves, with a first groove configured to receive a protruding element (or an end) of a first side of the assay bar, and a second groove configured to receive a protruding element (or an end) of a second side of the assay bar.
    Type: Grant
    Filed: August 2, 2011
    Date of Patent: April 15, 2014
    Inventor: Pierre F. Indermuhle
  • Patent number: 8685717
    Abstract: An apparatus comprising an assembly and a cover. The assembly comprises a sample block configured to receive a microtiter tray having a plurality of vials, each vial comprising a cap having a surface and a perimeter. The cover comprises a platen, vertically and horizontally displaceable in relationship to the sample block, the platen having a plurality of recesses. Each recess corresponds to a respective vial and is shaped and arranged to clear the surface of its respective cap when the cover is displaced onto the sample block. The cover includes a skirt in contact with and surrounding a perimeter of the platen. The skirt is configured for mating with a perimeter of a microtiter tray when a microtiter tray is received in the sample block, and also configured such that the cover contacts a microtiter tray when a microtiter tray is received in the sample block.
    Type: Grant
    Filed: January 17, 2007
    Date of Patent: April 1, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: John G. Atwood, Adrian Fawcett, Keith S. Ferrara, Paul M. Hetherington, Richard W. Noreiks, Douglas E. Olsen, John R. Widomski, Charles M. Wittmer
  • Patent number: 8663979
    Abstract: A culture apparatus comprising: an inner box configured to form a culture chamber; an outer box configured to cover the inner box; a fan configured to circulate gas inside the culture chamber through an air passage provided in the inner box within the culture chamber; a first through hole configured to penetrate a wall configuring a part of the air passage in the inner box; a second through hole configured to penetrate the wall and disposed at a position at which a flow velocity of the gas circulated through the air passage by the fan is lower than a flow velocity of the gas around the first through hole; a connecting pipe configured to connect the first through hole and the second through hole outside of the inner box; and a sensor configured to detect a concentration of the gas flowing in the culture chamber.
    Type: Grant
    Filed: June 19, 2012
    Date of Patent: March 4, 2014
    Assignee: Panasonic Healthcare Co., Ltd.
    Inventors: Kuniyoshi Kobayashi, Hiroki Busujima, Yasuhiro Kikuchi, Yuichi Tamaoki, Shinji Sugimoto, Tetsuo Sakurai
  • Patent number: 8585574
    Abstract: An infant warmer includes an infant enclosure defining an infant compartment, and a heating system pneumatically coupled with the infant compartment. The heating system includes a heater configured to selectively transfer heat to the infant compartment, and a thermal storage device configured to store heat from the heater and to selectively transfer said stored heat to the infant compartment. The infant warmer also includes a controller operatively connected to the heating system. The controller is configured to regulate the transfer of heat from the thermal storage device such that a target temperature is maintained within the infant compartment.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: November 19, 2013
    Assignee: General Electric Company
    Inventors: Lawrence Guy Ten Eyck, Steven Mitchell Falk
  • Patent number: 8574516
    Abstract: An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat sink and a tube rack. The heat sink has a main body with a through hole for insertion of the test tube. The through hole has a relatively big diameter section and a relatively small diameter section located below the relatively big diameter section. The tube rack is mounted on the heat sink for insertion of the test tube. The apparatus can ensure that the temperature at the liquid level of the reaction mixture is lower than the temperature suitable for conducting the primer annealing step in the polymerase chain reaction process.
    Type: Grant
    Filed: December 13, 2011
    Date of Patent: November 5, 2013
    Assignee: Genereach Biotechnology Corp.
    Inventors: Ping-Hua Teng, Cheng Su
  • Patent number: 8557569
    Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.
    Type: Grant
    Filed: July 3, 2008
    Date of Patent: October 15, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Steven J. Boege, Howard G. King, Eugene F. Young, Johannes P. Sluis, Mark F. Oldham
  • Patent number: 8557572
    Abstract: A culture apparatus comprising: a fan configured to circulate air inside a culture chamber in a predetermined direction in the culture chamber; a duct configured to guide air circulating inside the culture chamber along a wall face inside the culture chamber from an upper part to a lower part of the culture chamber; and a gas generator detachable from a predetermined position at a lower part of the duct, the gas generator being configured to take in air discharged from a lower part of the duct and discharge together with the air taken in a hydrogen peroxide gas to sterilize the inside of the culture chamber generated from a hydrogen peroxide solution into the culture chamber.
    Type: Grant
    Filed: June 19, 2012
    Date of Patent: October 15, 2013
    Assignee: Panasonic Healthcare Co., Ltd.
    Inventors: Kuniyoshi Kobayashi, Hiroki Busujima, Yasuhiro Kikuchi, Yuichi Tamaoki, Shinji Sugimoto, Tetsuo Sakurai
  • Patent number: 8549768
    Abstract: A method for producing a fog of ice nuclei by introducing a cryogenic fluid and a humid gas stream into a freeze dryer. The cryogenic fluid and humid gas stream will form ice nuclei having a preferred size for introduction into vials contained in the freeze dryer.
    Type: Grant
    Filed: March 11, 2011
    Date of Patent: October 8, 2013
    Assignee: Linde Aktiengesellschaft
    Inventors: Prerona Chakravarty, Ron C. Lee
  • Patent number: 8524490
    Abstract: Provided herein is a portable thermocycler, comprising: (i) a case; (ii) a rotary plate in the case; (iii) a plurality of heating blocks arranged in a geometric pattern disposed on the rotary plate; and (iv) at least one vessel adapted to move and contact at least two of the plurality of heating blocks; wherein each of the heating blocks comprises a heating plate maintained at a set temperature over a thermally insulating material; wherein the geometric pattern comprises a number of center heating blocks arranged in a shape defining a polygon and a number of outside heating blocks disposed around the periphery of the rotary plate; and wherein the rotary plate includes a plurality of rotating wheels adapted to rotate at least one of the vessels into contact with each of the heating blocks.
    Type: Grant
    Filed: September 16, 2009
    Date of Patent: September 3, 2013
    Assignees: X-Bar Diagnostic Systems, Inc.
    Inventors: James H. Lipscomb, Peter Blacklin, Michael Keating, Robert Bernstine, Richard Raffauf, Jr.
  • Patent number: 8501461
    Abstract: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: August 6, 2013
    Assignee: Gen-Probe Incorporated
    Inventors: Byron J. Knight, Haitao Li
  • Patent number: 8492138
    Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.
    Type: Grant
    Filed: September 12, 2012
    Date of Patent: July 23, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Steven J. Boege, Howard G. King, Eugene F. Young, Johannes P. Sluis, Mark F. Oldham
  • Patent number: 8445261
    Abstract: An apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises an imaging mechanism for automatically determining the state of an individual cell of the cells over time. The imaging mechanism is in communication with the incubating mechanism.
    Type: Grant
    Filed: January 7, 2011
    Date of Patent: May 21, 2013
    Assignee: University of Pittsburgh
    Inventors: Joel S. Greenberger, Paul A. DiMilla, Michael M. Domach, Raymond K. Houck
  • Patent number: 8420383
    Abstract: An incubator is fitted with an enclosure which surrounds the incubator. A cold air generation system supplies relatively cold air to a space formed between enclosure and the incubator. The system supplying the cold air can be embodied in a cart or base upon which the incubator sits. The enclosure is also placed on the base in a manner such that it substantially envelops the incubator. The top of the cart has openings for egress of cold air and return of air so that air may circulate in a closed loop. The incubator, cold air supply system and enclosure present a solution for incubating samples in a nominal 20-25° C. temperature environment. The enclosure, which is preferably insulated, may include a void or opening which exposes doors or other access device of the incubator, allowing direct access to the samples in the incubator while the rest of the incubator is enveloped by the enclosure.
    Type: Grant
    Filed: September 9, 2008
    Date of Patent: April 16, 2013
    Assignee: Biomerieux, Inc.
    Inventors: Mark J. Fanning, Jacky Yam, David M. Robbins, Kevin G. Ketsenburg, James C. Bishop
  • Patent number: 8409854
    Abstract: A bioreactor comprising a reactor chamber defined by reactor walls and a reactor opening, optionally with means penetrating the reactor opening in order to supply gases and/or liquids, for taking samples, for supplying microorganisms or cells, for the introduction of measuring probes, for the addition of additives or sterilizing agents and/or for emptying the bioreactor, with means for sealing the reactor opening, wherein at least one inflatable eddy current breaker is installed in the reactor chamber.
    Type: Grant
    Filed: July 31, 2001
    Date of Patent: April 2, 2013
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Ursula Erhardt, Christoph Erhardt
  • Patent number: 8409846
    Abstract: Compositions, methods, systems/devices and media are provided for maintaining a harvested organ in a functioning and viable state prior to implantation. The organ perfusion apparatus includes a preservation chamber for storing the organ during the preservation period. A perfusion circuit is provided having a first line for providing an oxygenated fluid to the organ, and a second line for carrying depleted fluid away from the organ. The perfusion apparatus also includes a device operably associated with the perfusion circuit for maintaining the organ at a substantially normothermic temperature.
    Type: Grant
    Filed: February 17, 2005
    Date of Patent: April 2, 2013
    Assignee: The United States of America as represented by the Department of Veteran Affairs
    Inventors: Waleed Hassanein, Shukri F. Khuri, Michael D. Crittenden, Vladimir Birjinuik
  • Patent number: 8372359
    Abstract: A vial retainer system for an automated processing apparatus. The vial retainer system includes a track mounted to the processing apparatus, a rack adapted to slide in a longitudinal direction on the track, and a vial retainer positioned over the rack. The rack is movable in a distal direction to install the rack on the processing apparatus, and in a proximal direction, opposite the distal direction, to remove the rack from the processing apparatus. The rack is adapted to hold one or more vials in an upright orientation. The vial retainer has one or more a sloped surfaces inclined such that a distal end of each sloped surface is closer to the track than a proximal end of each sloped surface.
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: February 12, 2013
    Assignees: Qiagen Gaithersburg, Inc.
    Inventors: Bert Jungheim, Andrew Leonard
  • Patent number: 8372634
    Abstract: A storage-access apparatus includes a first support device including a first base; at least one first storage-access device and at least one first storage device, disposed on the first base and arranged in a first circle; a second support device including a second base; at least one second storage-access device and at least one second storage device, disposed on the second base and arranged in a second circle; and a transporting device, wherein the first storage-access device, the first storage device, the second storage-access device and the second storage device each has plural compartments, the transporting device corresponds to the compartments, and the first storage-access device and the first storage device can rotate relative to the second storage-access device and the second storage device. Accordingly, the storage-access apparatus can be employed in depositing and picking up products independently. Also disclosed is a storage system including, among others, the storage-access apparatus.
    Type: Grant
    Filed: November 21, 2008
    Date of Patent: February 12, 2013
    Assignee: Healthbanks Biotech Co., Ltd.
    Inventors: Shang-Chih Lin, Jia-You Chen
  • Patent number: 8298815
    Abstract: Systems and methods of sample processing and temperature control are disclosed. The invention may especially relate to temperature control, and may in some embodiments be methods of temperature control of an automated sample processing system and methods of automated sample processing. Specifically, the present invention provides temperature control in relation to sample processing systems and methods of processing samples, and in some embodiments provides temperature control in relation to sample carriers and processing materials such as reagents. Corresponding systems and devices are disclosed, including sample processing systems (1), sample carrier temperature regulation systems (60), reagent temperature regulation systems, sample processing control systems, and temperature regulation devices, among other embodiments.
    Type: Grant
    Filed: December 22, 2003
    Date of Patent: October 30, 2012
    Assignee: DAKO Denmark A/S
    Inventors: Kristopher Buchanan, Marc Key, John Favuzzi, Rosanne Welcher, Benno Guggenheimer, Robert Clark, Michael Barber, Bob Lathrop
  • Patent number: 8298814
    Abstract: A method for the degradation of polycyclic aromatic compounds is disclosed that involves dissolving ozone in a bipolar solvent comprising a non-polar solvent in which is of sufficiently non-polar character to solubilized the polycyclic aromatic compounds, and a polar-water-compatible solvent which is fully miscible with the non-polar solvent to form a single phase with the non-polar solvent. The bipolar solvent with dissolved ozone is contacted with the polycyclic aromatic compounds to solubilize the polycyclic aromatic compounds and react the dissolved polycyclic aromatic compounds with the ozone to degrade the dissolved polycyclic aromatic compounds to oxygenated intermediates. The bipolar solvent is then mixed with sufficient water to form separate non-polar and polar phases, the non-polar phase comprising the non-polar solvent and the polar phase comprising the non-polar solvent and the oxygenated intermediates.
    Type: Grant
    Filed: November 6, 2000
    Date of Patent: October 30, 2012
    Assignees: University of Utah
    Inventors: P. K. Andy Hong, David A. Wavrek, Jiun-Chi Chao, Yu Zeng
  • Patent number: 8293471
    Abstract: A thermal cycle system and method suitable for mass production of DNA comprising a temperature control body having at least two sectors. Each sector has at least one heater, cooler, or other means for changing temperature. A path traverses the sectors in a cyclical fashion. In use, a piece of tubing or other means for conveying is placed along the path and a reaction mixture is pumped or otherwise moved along the path such that the reaction mixture is repetitively heated or cooled to varying temperatures as the reaction mixture cyclically traverses the sectors. The reaction mixture thereby reacts to form a product. In particular, polymerase chain reaction reactants may continuously be pumped through the tubing to amplify DNA. The temperature control body is preferably a single aluminum cylinder with a grooved channel circling around its exterior surface, and preferably has wedge-shaped or pie-shaped sectors separated by a thermal barrier.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: October 23, 2012
    Assignee: Marshall University Research Corporation
    Inventors: Derek A. Gregg, Elizabeth E. Murray, Michael L. Norton, Justin T. Swick, Herbert Tesser
  • Patent number: 8277763
    Abstract: The present invention relates to a method of processing analyte using a portable incubator apparatus. The incubator apparatus 10 has a plurality of cavities 20 each configured to receive analyte to be incubated. The method comprises: receiving analyte in each of the plurality of cavities; incubating the analyte in the plurality of cavities, the incubator apparatus being operable to control temperatures of analyte contained in the plurality of cavities independently of each other; and moving the incubator apparatus from a first location to a second location while the analyte is being incubated, the incubator apparatus being configured to maintain desired incubation conditions independently of a supply of electrical power and apparatus external to the incubator apparatus as the incubator apparatus is being moved.
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: October 2, 2012
    Assignee: Centeo Biosciences Limited
    Inventors: Philipp Steinmann, Gabriela Juárez Martinez
  • Patent number: 8247221
    Abstract: A thermal cycling device for thermally cycling samples of biological material contained in a microcard having a top and bottom surface. The thermal cycling device can include a sample block having an upper surface configured for engaging the bottom surface of a microcard, a vacuum device, and a temperature control system operatively connected with the sample block. The upper surface of the sample block may include a plurality of channels, the channels defining spaces between the sample block and the bottom surface of a microcard that may be positioned thereon. The vacuum device may be in fluid communication with the sample block for drawing gas out of the spaces defined by the channels in the sample block. The vacuum device may be configured for substantially maintaining a vacuum between the sample block and microcard so that a retention force is imparted on the microcard to urge the microcard toward the sample block.
    Type: Grant
    Filed: December 3, 2010
    Date of Patent: August 21, 2012
    Assignee: Applied Biosystems, LLC
    Inventor: Adrian Fawcett
  • Patent number: 8246919
    Abstract: A rack for a plurality of capped tubes, including a frame having a longitudinally extending bottom base member having a row of spaced open top recesses adapted to receive the capped tubes. Vertical supports at the ends of the row of recesses fix a cover member above the base member. The cover member has openings therethrough smaller than the capped tubes. A wall is along one longitudinal side between the base member and the cover member, with the other longitudinal side between the base member and the cover member being open. Support fingers extend horizontally from the wall toward the open longitudinal side and are vertically aligned between the recesses to secure capped tubes in a generally vertical direction in a longitudinally extending vertical plane. The base member and cover member are vertically spaced a distance sufficient to permit tipped capped tubes to be moved into the frame with the tipped tubes then dropped into the recesses and tipped back to an upright vertical position.
    Type: Grant
    Filed: September 21, 2006
    Date of Patent: August 21, 2012
    Assignee: Abbott Laboratories
    Inventors: Stephen L. Herchenbach, E. Kim Pavlovic
  • Patent number: 8241892
    Abstract: The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
    Type: Grant
    Filed: April 2, 2002
    Date of Patent: August 14, 2012
    Assignee: University of Pittsburgh
    Inventors: Joel S. Greenberger, Paul A. DiMilla, Michael M. Domach, Raymond K. Houck
  • Patent number: 8227239
    Abstract: A culture apparatus comprising: a housing including a culture chamber inside, culture being cultured in the culture chamber; a door configured to seal the culture chamber; an ultraviolet lamp configured to generate an ultraviolet ray for disinfecting air in the culture chamber; a lighting device configured to allow the ultraviolet lamp to be on for a predetermined time period, when the air in the culture chamber is disinfected; a detecting device configured to detect deterioration of disinfection capability of the ultraviolet lamp; and a controller configured to correct the predetermined time period, during which the ultraviolet lamp is on, so as to be longer according to the deterioration of the disinfection capability of the ultraviolet lamp detected by the detecting device.
    Type: Grant
    Filed: February 10, 2010
    Date of Patent: July 24, 2012
    Assignee: Panasonic Healthcare Co., Ltd.
    Inventors: Hiroki Busujima, Yasuhiro Kikuchi, Kenichi Ito, Kuniyoshi Kobayashi
  • Patent number: 8216829
    Abstract: This invention relates to a device intended for the culture of cells or micro-organisms, characterised by the fact that it comprises a chamber (100) composed of a tank (110) and a lid (150), adapted so that when in the closed state, it defines a sealed volume under a controlled pressure, and an assembly (200) designed to be placed inside the chamber (100), while being removable from it when the lid (150) is opened, said assembly comprising (200) a support frame (210) and a plurality of plates (250) carried by the support frame (210), the chamber (100) also comprising means designed to successively sterilise its content, for seeding a culture medium placed in the trays (250) and a means for controlling the atmosphere in the chamber (100), to enable the culture of cells or micro-organisms.
    Type: Grant
    Filed: May 18, 2007
    Date of Patent: July 10, 2012
    Assignee: Centre National de la Recherche Scientifique (CNRS)
    Inventors: Jamal Ouazzani, Sylvie Cortial, Didier Sergent, Philippe Lopes
  • Patent number: 8216830
    Abstract: A culture apparatus includes a culture chamber in which culture is cultured, a shelf plate included in the culture chamber, on which the culture is placed, and shelf rests in a left-and-right pair configured to support the shelf plate and a plurality of shelf supports configured to support the shelf rests. The shelf rests each include one piece supporting the shelf plate and the other piece supported by the shelf supports. The shelf rests are in an L-shape in a cross-sectional manner whose angle between the one piece and the other piece is an acute angle. The one piece is in line contact with a lower face of the shelf plate at an end of the one piece extending obliquely upward from a side face of the culture chamber toward the inside of the culture chamber. The culture chamber is supplied with sterilizing gas to be sterilized.
    Type: Grant
    Filed: February 17, 2010
    Date of Patent: July 10, 2012
    Assignee: Sanyo Electric Co., Ltd.
    Inventors: Kuniyoshi Kobayashi, Hiroki Busujima, Yasuhiro Kikuchi, Yuichi Tamaoki, Shinji Sugimoto, Tetsuo Sakurai
  • Patent number: 8206976
    Abstract: The present invention enables to keep the culture vessel clean with the clean air without touching the open air in which floating bacteria exist, and to culture cells with taking in and out the culture apparatus under the environment with low damages for the cells in constant temperature. The present invention is the culture vessel box which has one or more culture vessels, has the heater, and has the door outside which the good adhesive resin film is stuck, the culture apparatus which has various apparatuses including a heater and culture cells, and has the door outside which the resin film is stuck similarly to the culture vessel box and has its storing chamber.
    Type: Grant
    Filed: May 31, 2007
    Date of Patent: June 26, 2012
    Assignee: Hitachi, Ltd.
    Inventors: Toyoshige Kobayashi, Kazutoshi Kan
  • Patent number: 8192982
    Abstract: A tissue culture microscope apparatus includes a culture unit that includes a chamber in which a specimen is put, and maintains the chamber at a predetermined temperature to culture the specimen; an observation unit that forms an observation image of the specimen put in the chamber; and a liquid supply unit that stores a liquid in a protrusion portion penetrating into a wall of the chamber and protruding to an inside of the chamber, matches a temperature of the liquid with the temperature of the chamber, and injects the liquid from the protrusion portion to the specimen.
    Type: Grant
    Filed: September 18, 2006
    Date of Patent: June 5, 2012
    Assignee: Olympus Corporation
    Inventors: Kazuhiro Hasegawa, Akitsugu Kagayama, Atsuhiro Tsuchiya, Ryuichi Hirano, Kenichi Koyama, Hideaki Endo
  • Patent number: 8186775
    Abstract: An arm portal for ingress and egress within an enclosed, controlled environment, such as an incubator, employs asymmetric orifices to obtain superior arm mobility. The hand/arm portals along with the pass box can be manufactured into a one-piece injection molded assembly that is easily attached to the front face of an enclosed anaerobic environment.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: May 29, 2012
    Inventor: Dan M. Sheldon
  • Patent number: 8142740
    Abstract: A rack for an automated processing system. The rack includes a number of wells adapted to hold at least a first sample container having a first size, and a second sample container having a second size. The second size, which may be a diameter, a height, or both, is substantially different from the first size. A structure joins the wells. The rack is adapted to fit in an automated processing system that is adapted to remove both the first sample container and the second sample container from the rack.
    Type: Grant
    Filed: November 12, 2009
    Date of Patent: March 27, 2012
    Assignee: Qiagen Gaithersburg, Inc.
    Inventors: Brian Austin Self, James Hal Godsey
  • Patent number: 8137622
    Abstract: A device for biochip hybridization or washing is provided, which comprises a carousel (12), a translational movement controller, a revolving movement controller, and optionally a heating chamber (13). The revolving movement controller controls the revolving movement of the carousel and allows it to move in a wobbling fashion, allowing liquid movement of the hybridization or washing boxes (11) during hybridization and/or washing of biochips on the carousel. The translational movement controller brings the carousel back to horizontal position once the revolving movement controller stops, thereby ensures that the liquid do not spill out. The heating chamber circulates hot air within the device, thereby ensures that the hybridization and washing in a thermostatic condition.
    Type: Grant
    Filed: October 9, 2007
    Date of Patent: March 20, 2012
    Assignees: CapitalBio Corporation, Tsinghua University
    Inventors: Hang Li, Jianxin Ye, Xingling Al, Xianhua Wang, Liang Zhang, Jing Cheng
  • Patent number: 8110394
    Abstract: A culture microscope apparatus has an illumination unit to apply excitation light to the specimen, a specimen observing portion to observe light generated from the specimen due to the excitation light, and a dimmer unit to dim the excitation light that has penetrated the specimen.
    Type: Grant
    Filed: May 7, 2010
    Date of Patent: February 7, 2012
    Assignee: Olympus Corporation
    Inventors: Kazuhiro Hasegawa, Kenichi Koyama, Atsuhiro Tsuchiya, Atsushi Yonetani
  • Patent number: 8097449
    Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the cassette and transporter with heat transfer surfaces arranged to transfer heat between a cooling source in said transporter and the heat transfer surfaces of the cassette.
    Type: Grant
    Filed: February 16, 2010
    Date of Patent: January 17, 2012
    Assignee: Organ Recovery Systems
    Inventors: Jeffrey C Garland, Douglas Schein, David Walters Wright
  • Patent number: 8080411
    Abstract: Disposable units in current use for performing PCR are limited by their heat block ramping rates and by the thermal diffusion delay time through the plastic wall as well as by the sample itself. This limitation has been overcome by forming a disposable plastic chip using a simple deformation process wherein one or more plastic sheets are caused, through hydrostatic pressure, to conform to the surface of a suitable mold. After a given disposable chip has been filled with liquid samples, it is brought into close contact with an array of heating blocks that seals each sample within its own chamber, allowing each sample to then be heat treated as desired.
    Type: Grant
    Filed: October 20, 2008
    Date of Patent: December 20, 2011
    Assignees: Agency for Science, Technology and Research, National University of Singapore
    Inventors: Yubo Miao, Yu Chen, Tit Meng Lim, Chew Kiat Heng
  • Patent number: 8080409
    Abstract: Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.
    Type: Grant
    Filed: June 4, 2010
    Date of Patent: December 20, 2011
    Assignee: 3M Innovative Properties Company
    Inventors: James E. Aysta, William Bedingham, Barry W. Robole
  • Patent number: 8017383
    Abstract: A solution temperature control device in a biological cell observing chamber (30) used for the detection of chemotaxis and chemotactic cell separator, comprising a first temperature controller (62) and a second temperature controller (63). The first temperature controller (62) measures the temperature of a solution filled in a pair of wells and a flow passage in the chamber and controls the temperature to a specified temperature, and the second temperature controller (63) measures the temperature of a heating part (64) which heats the chamber (30) from the outside to indirectly heat the solution filled in the pair of wells and the flow passage and controls the temperature to a specified preheat temperature. Since the state and quantity of cells moving from one well to the other through the flow passage while holding the temperature of the solution at a specified temperature can be accurately observed and measured, accuracy for controlling the temperature of the solution can be remarkably increased.
    Type: Grant
    Filed: September 13, 2004
    Date of Patent: September 13, 2011
    Assignee: Hirata Corporation
    Inventors: Kouichi Noguchi, Kazuyuki Matsumura, Takesi Mitsunaga, Shiro Kanegasaki
  • Patent number: 8008066
    Abstract: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.
    Type: Grant
    Filed: March 10, 2006
    Date of Patent: August 30, 2011
    Assignee: Gen-Probe Incorporated
    Inventors: Gary D. Lair, Thanh N. Nguyen, Haitao Li, Florence F. Li, Byron J. Knight, Robert E. Heinz, Jerzy A. Macioszek, Christopher B. Davis, Robert F. Scalese
  • Patent number: 7972579
    Abstract: The invention relates to a device for the automatic opening and closing of reaction vessels. It comprises a holding device for the non-rotatable holding of one or more reaction vessels, a gripper for the gripping of a lid for the reaction vessel, wherein the gripper has gripping jaws to take hold of the lid, and a holding arm with a rotating mechanism for rotatable holding of the gripper. The gripping jaws are arranged in such a way that, when the lid is inserted into the area between the gripping jaws, it is held by the latter through frictional contact, and the gripper has no active operating device for opening and closing the gripping jaws.
    Type: Grant
    Filed: July 1, 2004
    Date of Patent: July 5, 2011
    Assignee: F. Hoffmann-La Roche AG
    Inventor: Wolfgang Brunner
  • Patent number: 7947237
    Abstract: The invention provides a rack system containing an adapter for convenient application of small volumes of sample in a vessel to analysis.
    Type: Grant
    Filed: July 6, 2005
    Date of Patent: May 24, 2011
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Renato Belz, Pius Emmenegger