Achromobacter Patents (Class 435/824)
  • Patent number: 8329422
    Abstract: The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CELLSPOTTER® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.
    Type: Grant
    Filed: November 2, 2010
    Date of Patent: December 11, 2012
    Assignee: Veridex LLC
    Inventors: Galla Chandra Rao, Christopher Larson, Madeline Repollet, Herman Rutner, Leon W. M. M. Terstappen, Shawn Mark O'Hara, Steven Gross
  • Patent number: 7358096
    Abstract: The immobilization of proteins at solid surfaces facilitated by incorporation of a polypeptide segment which is capable of adopting a folded structure is described. Materials comprising proteins so immobilized and uses thereof are also described.
    Type: Grant
    Filed: November 22, 2000
    Date of Patent: April 15, 2008
    Assignee: Conopco, Inc.
    Inventors: Steven Daryl Grant, Steven Howell, Stephen Wilson
  • Patent number: 6187583
    Abstract: A vessel for conducting blood cell agglutination assays is disclosed. A barrier retains reactants in an upper chamber during incubation, then, in response to a force, permits reagents to enter a lower chamber containing a matrix for separating agglutination.
    Type: Grant
    Filed: May 28, 1998
    Date of Patent: February 13, 2001
    Inventors: Walter Milchanoski, Milan Jorik, Kathleen J. Reis, Diane E. Bechtold, Linda Davis, Thomas M. Setcavage
  • Patent number: 6171823
    Abstract: The invention relates to a method of producing an extracellular protein in a bacterium provided with an inner and an outer cell membrane, the method comprising: (a) providing a recombinant vector including a DNA construct comprising a DNA sequence encoding the prepropeptide or part of the prepropeptide of a bacterial extracellular protease selected from the group consisting of Achromobacter lyticus protease I, Bacillus metalloproteases and Bacillus serine proteases preceding and operably connected to a DNA sequence encoding a desired protein, (b) transforming cells of a microorganism provided with an inner and outer cell membrane with the recombinant vector of step (a), (c) culturing the transformed cells of step (b) under conditions permitting expression of said DNA insert and leakage of the bacterial extracellular protease propeptide fused to the desired protein into the culture medium, and (d) recovering the resulting protein from the medium.
    Type: Grant
    Filed: June 2, 1997
    Date of Patent: January 9, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Helle Fabricius W├Âldike, Sven Hastrup
  • Patent number: 6083736
    Abstract: The present invention provides a bacterial microorganism, Ochrobactrum anthropi, having the ability to degrade or detoxify moniliformin or structurally related mycotoxins. The present invention further provides a method for detoxification of grain pre- or post-harvest using Ochrobactrum anthropi having the ability to degrade or detoxify moniliformin or derivatives or analogs of moniliformin.
    Type: Grant
    Filed: March 17, 1999
    Date of Patent: July 4, 2000
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Jon Duvick, Tracy A. Rood
  • Patent number: 6057147
    Abstract: The present invention is directed to an apparatus and method for enhanced bioremediation of hydrocarbons removed from a contaminated object comprising: (a) a basin for cleansing said hydrocarbon-contaminated object, said basin having a means for introducing a recycling bioremediating cleaning solution (NATURES WAY PC.TM.) for washing said object, a means for draining said solution from said basin into a biochamber reservoir and a means for screening particles from said solution upon entry into said reservoir; and (b) said reservoir having a means for temperature control between 90.degree. to 112.degree. F., means for aerating said solution, means for agitating said solution, an outlet means to a plurality of filters for filtering said solution, an inlet means from said filters and means for removing filtered sediments.
    Type: Grant
    Filed: January 21, 1997
    Date of Patent: May 2, 2000
    Inventors: Bert A. Overland, Gary M. Mitchell
  • Patent number: 5958758
    Abstract: A process for treating an animal waste in a waste holding facility to reduce sulfides and enhance efficient degradation of large amounts of organic matter with reduced odor. The process includes a first inoculation with sulfide-utilizing bacteria and a second inoculation with organic digesting bacteria and lytic enzymes. The second organic digesting inoculation is performed at a time when the sulfide content of the animal waste in the facility is adequately reduced to support organic digesting bacterial growth and efficient degradation of organic matter.
    Type: Grant
    Filed: August 4, 1997
    Date of Patent: September 28, 1999
    Assignee: Biosun Systems Corporation
    Inventors: Guy W. Miller, Gregory Scott Patterson
  • Patent number: 5869322
    Abstract: The invention provides a method of textile printing using microorganisms with less limitation to dyes, with less damage on base materials, and with less blurring of color borders. The method is capable of producing a complex, fine pattern; capable of realizing colored discharge printing with brilliant colors; and capable of readily producing an ombre pattern. The invention also provides a microorganism for decolorization of azo-system dye and use in the textile printing method.
    Type: Grant
    Filed: November 13, 1996
    Date of Patent: February 9, 1999
    Assignee: Yushiro Chemical Industry Co., Ltd.
    Inventors: Wataru Sugiura, Keiko Nagai, Tadashi Yokoyama
  • Patent number: 5700666
    Abstract: A process for the production of arabinonucleotides of general formula I ##STR1## in which X represents a hydrogen atom or a fluorine atom, is described, which is characterized in that an arabinonucleoside of general formula II ##STR2## in which X has the above-mentioned meaning, is fermented in the presence of an aryl phosphate of general formula III ##STR3## in which Y symbolizes a hydrogen atom or a nitro group andZ symbolizes two hydrogen atoms or two alkali metal atoms,with a microorganism that is capable of phosphorylating nucleosides.
    Type: Grant
    Filed: September 4, 1996
    Date of Patent: December 23, 1997
    Assignee: Schering Aktiengesellschaft
    Inventors: Heidi Hummel-Marquardt, Thomas Schmitz, Mario Kennecke, Alfred Weber
  • Patent number: 5693539
    Abstract: Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.
    Type: Grant
    Filed: May 31, 1994
    Date of Patent: December 2, 1997
    Inventors: Stefan Miltenyi, Andreas Radbruch, Walter Weichel, Werner Muller, Christoph Gottlinger, Klaus Ludwig Meyer
  • Patent number: 5639670
    Abstract: A method is revealed for the quantitative determination of the proportion of the free form of a thyroid hormone ligand in a sample of a biological fluid in which the ligand is present partly in the free form and also partly in a form in which it is bound to physiological binding proteins. In the first step, a ligand derivative of the thyroid hormone with an immunoglobulin, a labelled specific binder, and a test tube whose walls are coated with an excess of a protein material are provided. In the second step, a sample of the biological fluid containing an unknown amount of the free thyroid hormone ligand, a solution containing a known amount of the ligand derivative, and a solution containing a known, less than stoichiometric amount of the labelled specific binder are added to the test tube to form a liquid reaction mixture.
    Type: Grant
    Filed: January 17, 1995
    Date of Patent: June 17, 1997
    Assignee: B.R.A.H.M.S. Diagnostica GmbH
    Inventors: Andreas Bergmann, Joachim Struck
  • Patent number: 5508194
    Abstract: A mixed bacteria culture for biodegrading polycyclic aromatic hydrocarbon contaminants includes Achromobacter sp. and Mycobacterium sp. which have been grown together and gradually acclimated to utilize polycyclic aromatic hydrocarbons as a primary food source. The mixed bacteria culture can be utilized for in situ or ex situ bioremediation of contaminated soil, or in any of various conventional bioreactors to treat contaminated liquids such as landfill leachates, groundwater or industrial effluents. The bacteria, the nutrients used to sustain growth of the bacteria, and the products of the biodegradation of the polycyclic aromatic or other hydrocarbons are all substantially harmless to the environment. The mixed bacteria can be utilized in the presence of oxygen, or hydrogen peroxide can be used alone or in combination with oxygen as an effective alternative electron acceptor. The mixed bacteria culture of Achromobacter sp. and Mycobacterium sp.
    Type: Grant
    Filed: May 18, 1995
    Date of Patent: April 16, 1996
    Assignee: The University of Akron
    Inventors: Sunggyu Lee, Teresa J. Cutright
  • Patent number: 5436145
    Abstract: 6-Hydroxy nitrogen-containing 6-membered ring compounds of the following general formula (II): ##STR1## wherein R.sup.1 represents carboxy group, carbamoyl group, cyano group, formyl group, C.sub.1 -C.sub.5 hydroxyalkyl group, C.sub.2 -C.sub.6 alkoxycarbonyl group, carboxyvinyl group, carboxymethyl group or oxime group, R.sup.2 represents hydrogen atom or carboxy group, and A represents carbon atom or nitrogen atom, can be prepared by reacting a nitrogen-containing 6-membered ring compounds of the following general formula (I): ##STR2## wherein R.sup.1, R.sup.2 and A are as defined in the general formula (II) above, with a microorganism or physico-chemically treated microorganism in an aqueous medium. Efficiency of the above reaction can be raised by conducting the reaction in the presence of phenazine methosulfate.
    Type: Grant
    Filed: May 20, 1994
    Date of Patent: July 25, 1995
    Assignee: Mitsubishi Kasei Corporation
    Inventors: Mari Yasuda, Haruyuki Ohkishi, Katsutoshi Sato, Yuuki Morimoto, Toru Nagasawa
  • Patent number: 5429935
    Abstract: Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (s) -2-hydroxy-4-phenyl-3-butenoic acid.
    Type: Grant
    Filed: August 5, 1993
    Date of Patent: July 4, 1995
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Ichiro Takase, Yoichiro Ueda, Yoshinori Kobayashi
  • Patent number: 5427944
    Abstract: A mixed bacteria culture for biodegrading polycyclic aromatic hydrocarbon contaminants includes Achromobacter sp. and Mycobacterium sp. which have been grown together and gradually acclimated to utilize polycyclic aromatic hydrocarbons as a primary food source. The mixed bacteria culture can be utilized for in situ or ex situ bioremediation of contaminated soil, or in any of various conventional bioreactors to treat contaminated liquids such as landfill leachates, groundwater or industrial effluents. The bacteria, the nutrients used to sustain growth of the bacteria, and the products of the biodegradation of the polycyclic aromatic or other hydrocarbons are all substantially harmless to the environment. The mixed bacteria can be utilized in the presence of oxygen, or hydrogen peroxide can be used alone or in combination with oxygen as an effective alternative electron acceptor. The mixed bacteria culture of Achromobacter sp. and Mycobacterium sp.
    Type: Grant
    Filed: May 24, 1994
    Date of Patent: June 27, 1995
    Inventors: Sunggyu Lee, Teresa J. Cutright
  • Patent number: 5344768
    Abstract: Pyrrolo-quinoline quinone (PQQ) is microbiologically produced by culturing a bacterium in a medium containing methanol, methylamine or a mixture thereof as a carbon source and recovering PQQ from the culture medium. The bacteria are strains of Methylobacterium, Ancylobacter, Hyphomicrobium, Xanthobacter, Thiobacillus, Microcyclus and Achromobacter.
    Type: Grant
    Filed: July 14, 1993
    Date of Patent: September 6, 1994
    Assignee: Mitsubishi Gas Chemical Co., Inc.
    Inventor: Teizi Urakami
  • Patent number: 5288620
    Abstract: Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid.
    Type: Grant
    Filed: May 20, 1992
    Date of Patent: February 22, 1994
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Ichiro Takase, Yoichiro Ueda, Yoshinori Kobayashi
  • Patent number: 5256544
    Abstract: A process is provided for solubilizing phosphate from phosphate containing ore by treatment with microorganisms which comprises forming an aqueous mixture of phosphate ore, microorganisms operable for solubilizing phosphate from the phosphate ore and maintaining the aqueous mixture for a period of time and under conditions operable to effect the microbial solubilization process. An aqueous solution containing soluble phosphorous can be separated from the reacted mixture by precipitation, solvent extraction, selective membrane, exchange resin or gravity methods to recover phosphate from the aqueous solution.
    Type: Grant
    Filed: January 27, 1992
    Date of Patent: October 26, 1993
    Assignee: EG&G Idaho, Inc.
    Inventors: Robert D. Rogers, James H. Wolfram
  • Patent number: 5256552
    Abstract: 2-Oxo-4-phenylbutyric acid is treated with a microorganism, which has been optionally treated, capable of asymmetrically reducing 2-oxo-4-phenylbutyric acid into either (R)-2-hydroxy-4-phenylbutyric acid or (S)-2-hydroxy-4-phenylbutyric acid, and the (R)-2-hydroxy-4-phenylbutyric acid or (S)-2-hydroxy-4-phenylbutyric acid thus produced is recovered to thereby give optically active 2-hydroxy-4-phenylbutyric acid.The optically active 2-hydroxy-4-phenylbutyric acid is an important intermediate in the synthesis of various drugs such as a remedy for hypertension.
    Type: Grant
    Filed: July 17, 1992
    Date of Patent: October 26, 1993
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Teruyuki Nikaido, Yoshinori Kobayashi
  • Patent number: 5248599
    Abstract: Disclosed is a DNA sequence containing a DNA segment coding for Achromobacter protease I (API) or variants thereof (referred to as T-API); a recombinant DNA constructed by introducing the DNA sequence in an expression vector so as to express the T-API; a transformant bearing the recombinant DNA; a process for producing the API which comprises cultivating the transformant, accumulating the T-API in a culture product, and recovering the same: and a protein of T-API. The cells transfected or transformed with the DNA sequence of the present invention allow for the production of a large amount of precursor protein of the T-API or the mature peptide.
    Type: Grant
    Filed: February 20, 1990
    Date of Patent: September 28, 1993
    Assignee: Waco Pure Chemical Industries, Ltd.
    Inventors: Fumio Sakiyama, Atsuo Nakata
  • Patent number: 5248608
    Abstract: Enzyme-catalysed preparation of an .alpha.-substituted optically active carboxylic acid, using enantioselective hydrolysis of a mixture of the enantiomers of the corresponding amide, an Ochrobactrum anthropi or an Klebsiella sp. being used as the enzyme. During the hydrolysis both the L-carboxylic acid and the remaining D-carboxylic acid amide are obtained with high optical purity and high yield, while the activity of the micro-organism is high. A large number of .alpha.-substituted carboxylic acid amides with a remarkable structural variety can be hydrolysed in this manner.
    Type: Grant
    Filed: January 10, 1992
    Date of Patent: September 28, 1993
    Assignee: DSM N.V.
    Inventors: Theodorus J. G. M. Van Dooren, Wilhelmus J. J. Van Den Tweel
  • Patent number: 5244803
    Abstract: There is disclosed a process for preparing optically active 3-phenylglycidic acid ester compound, which comprises permitting a culture broth, cells or treated cells of a microorganism having an ability of stereoselectively hydrolyzing a (2R, 3S)-3-phenylglycidic acid ester compound to act on a racemic 3-phenylglycidic acid ester compound which may also have a substituent on the phenyl group, thereby hydrolyzing the (2R, 3S) optically active isomer and separating and collecting the (2S, 3R) antipode from the reaction mixture.
    Type: Grant
    Filed: September 7, 1990
    Date of Patent: September 14, 1993
    Assignee: Tanabe Seiyaku Co., Ltd.
    Inventors: Takao Mori, Toshiyuki Furutani, Akio Nakao, Atsuhiko Tsujimura, Takeji Shibatani
  • Patent number: 5194380
    Abstract: Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid.
    Type: Grant
    Filed: March 1, 1990
    Date of Patent: March 16, 1993
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Ichiro Takase, Yoichiro Ueda, Yoshinori Kobayashi
  • Patent number: 5187093
    Abstract: Process for the production of L-carnitine utilizing a microorganism. The microorganism is cultivated with crotonobetaine and/or .gamma.-butyrobetaine in the presence of a growth substrate. The enriched L-carnitine is isolated. The microorganisms used are those which are capable of producing L-carnitine while not catabolizing it from crotonobetaine and/or .gamma.-butyrobetaine. The microorganisms can be obtained by the following selection method. Microorganisms which grow with betaine, .gamma.-butyrobetaine, crotonobetaine and L-carnitine as the C- and N-source are mutated in the conventional manner. From the culture obtained by cultivating of the mutated microorganisms, those microorganisms are selected which are stable, do not catabolize L-carnitine and do not grow on L-carnitine, crotonobetaine and .gamma.-butyrobetaine, but grow with betaine.
    Type: Grant
    Filed: March 15, 1990
    Date of Patent: February 16, 1993
    Assignee: Lonza Ltd.
    Inventors: Hans Kulla, Pavel Lehky
  • Patent number: 5151351
    Abstract: A process for the production of 6-hydroxynicotinic acid from nicotinic acid by means of enzymatic hydroxylation in the presence of microorganisms of the genera Pseudomonas, Bacillus or Achromobacter. By maintaining a specific concentration range during the addition of nicotinic acid, the biomass formation can take place in the same process step as the product formation, without product losses occurring by further decomposition.
    Type: Grant
    Filed: May 24, 1991
    Date of Patent: September 29, 1992
    Assignee: Lonza Ltd.
    Inventors: Frans Hoeks, Daniel Venetz
  • Patent number: 5089176
    Abstract: An optically active monoester of the formula of ##STR1## where R is C.sub.1 -C.sub.15 alkyl is disclosed. The monoester is produced by transesterification of cis-endo-bicyclo [2,2,2] oct-5-ene-2,3-dimethanol with a fatty acid alkyl or vinyl ester, or triglyceride by using an esterase.
    Type: Grant
    Filed: August 17, 1990
    Date of Patent: February 18, 1992
    Assignee: Chisso Corporation
    Inventors: Tetsuya Ogawa, Naoyuki Yoshida, Seiichi Takano, Kunio Ogasawara
  • Patent number: 5082777
    Abstract: Process for the production of 6-hydroxynicotinic acid from nicotinic acid. The hydroxylation is carried out enzymatically in the presence of a microorganism of the species Pseudomonas, Bacillus or Achromobacter, for example, Achromobacter xylosoxydans. Preferably the enzymatic hydroxylation is carried out at 20.degree. to 40.degree. C. and a pH of 5.5 to 9.0 under aerobic conditions. Also, preferably a 0.1 percent by weight solution up to a saturated (preferably a 0.5 to 10 percent by weight) nicotine acid solution is used.
    Type: Grant
    Filed: February 14, 1985
    Date of Patent: January 21, 1992
    Assignee: Lonza Ltd.
    Inventors: Pavel Lehky, Hans Kulla, Stephane Mischler
  • Patent number: 5081024
    Abstract: An efficient process of optical resolution is provided for producing an L-amino acid represented by the following formula (I): ##STR1## wherein R is --CH.sub.2 CO.sub.2 H, --CH.sub.2 CONH.sub.2, --CO.sub.2 H or ##STR2## in which R.sup.1 is hydrogen atom, an alkyl group having 1 to 10 carbon atoms or a halogen-substituted alkyl group having 1 to 10 carbon atoms. The process comprises an optical resolution of an N-substituted carbonyl-D,L-amino acid represented by the formula (II): ##STR3## (wherein R is the same as defined above and R.sup.
    Type: Grant
    Filed: August 22, 1989
    Date of Patent: January 14, 1992
    Assignee: Nissan Chemical Industries, Ltd.
    Inventors: Masao Kuwahara, Michito Tagawa, Takashi Furusato, Hiroyuki Narushima, Shuzo Shinke
  • Patent number: 5077208
    Abstract: Disclosed is a process by which a gas containing nitric oxide is contacted with an anaerobic microbial culture of denitrifying bacteria to effect the chemical reduction of the nitric oxide to elemental nitrogen. The process is particularly suited to the removal of nitric oxide from flue gas streams and gas streams from nitric acid plants. Thiobacillus dentrificians as well as other bacteria are disclosed for use in the process.
    Type: Grant
    Filed: June 4, 1990
    Date of Patent: December 31, 1991
    Assignee: ABB Environmental Services Inc.
    Inventor: Kerry L. Sublette
  • Patent number: 5063054
    Abstract: A water soluble and ethanol insoluble extract of microbial product obtained by the cultivation of Achromobacter stenohalis is used to combat viral diseases.
    Type: Grant
    Filed: April 19, 1990
    Date of Patent: November 5, 1991
    Inventor: Joseph Chang
  • Patent number: 4877616
    Abstract: A water-soluble complex which is isolable from acid-precipitable material recovered from cultures of the bacterium, Achromobacter xerosis, and which is effective in inducing a variety of therapeutic responses commonly associated with biological responses modifiers and anti-inflammatory agents and which exhibits significant therapeutic activity in higher vertebrates at a dosage ranging from about 0.1 to about 25.0 mg/kg of body weight. Also disclosed are compositions containing such complexes, methods of preparation with such complexes and methods of treatment with compositions having such complexes.
    Type: Grant
    Filed: April 8, 1988
    Date of Patent: October 31, 1989
    Inventors: Elizabeth S. Boylan, Robert J. Boylan, Vincent Groupe, Robert W. Simpson
  • Patent number: 4746609
    Abstract: The present invention teaches restriction endonuclease Asp 718 which cleaves DNA in the palindromic recognition sequence at the sites indicated by the arrow: ##STR1## The endonuclease, obtained from Achromobacter species 718, DSM 2969, is useful in obtaining DNA fragments, e.g., for determining nucleotide sequences and other types of analysis of DNA.
    Type: Grant
    Filed: June 10, 1985
    Date of Patent: May 24, 1988
    Assignee: Boehringer Mannheim GmbH
    Inventors: Bryan Bolton, Michael J. Comer, Christoph Kessler, Georg Nesch
  • Patent number: 4708936
    Abstract: Process for the continuous production of L-carnitine by the microbiological method. A microorganism of the strain DSM No. 3225 (HK 1331b) type is cultivated in a bioreactor with crotonobetaine and/or .gamma.-butyrobetaine in the presence of a growth substrate. The culture fluid passes outside of the bioreactor in a circulation in which a separation of the cell is carried out. A quantity of cell-free solution, which is as large as the amount fed to the bioreactor as a substrate, is withdrawn from the bioreactor. The L-carnitine is separated from the cell-free solution.
    Type: Grant
    Filed: March 29, 1985
    Date of Patent: November 24, 1987
    Assignee: Lonza Ltd.
    Inventors: Hans Kulla, Pavel Lehky, Armand Squaratti
  • Patent number: 4634667
    Abstract: Process for preparing a heteropolysaccharide from strain NCIB 11883 and use of the latter e.g. as viscosity modifier in an aqueous system such as completion fluids, work over fluids, stimulation fluids, and preferably in drilling fluids and use in connection with well-treatments, and enhanced oil recovery.
    Type: Grant
    Filed: September 24, 1984
    Date of Patent: January 6, 1987
    Assignee: Shell Oil Company
    Inventors: John D. Linton, Michael W. Evans, Andrew R. Godley
  • Patent number: 4594321
    Abstract: Glycosylation or transglycosylation of a specified guanine derivative, namely 9-substituted or non-substituted guanine of formula [I] with a 3-deoxyribose donor such as 3'-deoxyadenosine in the presence of a nucleoside phosphorylase source such as of microorganism origin is disclosed.
    Type: Grant
    Filed: March 29, 1983
    Date of Patent: June 10, 1986
    Assignee: Yamasa Shoyu Kabushiki Kaisha
    Inventors: Tetsuro Fujishima, Shinji Sakata
  • Patent number: 4581332
    Abstract: A novel protease is described. The enzyme has the following characteristics: molecular weight: 30,000 (a gel filtration method using Sephadex G-75); isoelectric point, 5.3; pH reactivity: the optimum pH for its esterase activity is at pH 8.5 and the optimum pH for its amidase activity is at pH 9.0 respectively; substrate reactivity: it selectively and specifically hydrolyzes the ester bond and the amide bond derived from the carboxyl group of L-lysine; inhibitor: it is inhibited by diisopropyl phosphofluoride, tosyl-L-lysine chloro-methyl ketone and phenylmethylsulfonyl chloride. The protease is preferably produced by a microorganism belonging to the genus Achromobacter.
    Type: Grant
    Filed: September 28, 1983
    Date of Patent: April 8, 1986
    Assignee: Wako Pure Chemical Industries, Ltd.
    Inventors: Masami Soejima, Takeharu Masaki, Hideya Suzuki
  • Patent number: 4576914
    Abstract: A method of enhancing a fungus-lytic activity of .beta.-1,3-D-glucanase which comprises using said glucanase in the presence of one or more of the activators selected from the group consisting of sodium lauroylsarcosinate, polyoxyethylene alkylphenyl ether, polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylenealkyl ether, benzalkonium chloride, ammonium chloride, chlorhexidine glucuronate, methylparaben, propylparaben, trypsin, Pronase.RTM. and Alcalase.RTM..A method of enhancing a fungus-lytic activity of .beta.-1,3-D-glucanase which comprises using two .beta.-1,3-D-glucanases of different origins is also provided.
    Type: Grant
    Filed: October 17, 1983
    Date of Patent: March 18, 1986
    Assignee: Rohto Pharmaceutical Co., Ltd.
    Inventors: Akiyoshi Yoshida, Shigeru Kametaka, Shin'ichi Hayashi
  • Patent number: 4401757
    Abstract: A process for preparing B30-threonine-insulin which comprising reacting a des-B30-insulin with an excess amount of threonine derivative in the presence of an enzyme specifically acting on a lysine carbonyl in peptide bondings and produced by Achromobacter lyticus M 497-1.
    Type: Grant
    Filed: December 31, 1981
    Date of Patent: August 30, 1983
    Assignee: Shionogi & Co., Ltd.
    Inventors: Kazuyuki Morihara, Tatsushi Oka, Masami Soejima, Takeharu Masaki
  • Patent number: 4400465
    Abstract: A process for preparing a B30-threonine-insulin which comprising reacting a des-B30-insulin with an excess amount of threonine derivative in the presence of an enzyme specifically acting on the basic-amino-acid carbonyl in peptide bondings.
    Type: Grant
    Filed: December 31, 1981
    Date of Patent: August 23, 1983
    Assignee: Shionogi & Co., Ltd.
    Inventors: Kazuyuki Morihara, Tatsushi Oka, Hiroshige Tsuzuki
  • Patent number: 4387163
    Abstract: The enzyme cholesterol esterase is produced by cultivating a strain of the Achromobacter delicatulus type, isolated from vegetable debris and identified by the symbol NRRL B-12115. Under the described culture conditions, the enzyme is exocellular and therefore does not require extraction from the cells.In addition, the micro-organism has a duplication time less than that of moulds and Actinomycetes. Cholesterol esters of fatty acids can be hydrolysed by adding them to the culture medium before inoculating the strain.
    Type: Grant
    Filed: July 13, 1981
    Date of Patent: June 7, 1983
    Assignee: E.N.I. Ente Nazionale Idrocarburi
    Inventors: Pasquale Sacceddu, Vincenza Vitobello, Paolo Braduzzi, Nadia Cimini, Ludwig Degen
  • Patent number: 4335211
    Abstract: Disclosed are optically active acylated cephalosporin analogs which are useful as antibacterial agents and methods for preparing such compounds.
    Type: Grant
    Filed: November 13, 1980
    Date of Patent: June 15, 1982
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Yukio Hashimoto, Seigo Takasawa, Tadashi Hirata, Ikuo Matsukuma, Shigeo Yoshiie
  • Patent number: 4320197
    Abstract: A process for preparing a B30-threonine-insulin which comprising reacting a des-B30-insulin with an excess amount of threonine derivative in the presence of an enzyme specifically acting on a lysine carbonyl in peptide bondings and produced by Achromobacter lyticus M 497-1.
    Type: Grant
    Filed: April 9, 1980
    Date of Patent: March 16, 1982
    Assignee: Shionogi & Co., Ltd.
    Inventors: Kazuyuki Morihara, Tatsushi Oka, Masami Soejima, Takeharu Masaki
  • Patent number: 4302540
    Abstract: Disclosed are optically active cephalosporin analogs which are produced by optically selective deacylation of an optically inactive acylated analog. The compounds are useful as intermediates in the preparation of optically active acylated antimicrobial agents.
    Type: Grant
    Filed: October 24, 1980
    Date of Patent: November 24, 1981
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Tadashi Hirata, Yukio Hashimoto, Takehiro Ogasa, Shigeru Kobayashi, Akira Sato, Kiyoshi Sato, Seigo Takasawa
  • Patent number: 4302541
    Abstract: Optically active cephalosporin analogs are produced by optically selective deacylation of an optically inactive acylated analog. The compounds are useful as intermediates in the preparation of optically active acylated antimicrobial agents.
    Type: Grant
    Filed: November 13, 1980
    Date of Patent: November 24, 1981
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Tadashi Hirata, Yukio Hashimoto, Ikuo Matsukuma, Shigeo Yoshiie, Seigo Takasawa
  • Patent number: 4247645
    Abstract: A process for removing melamine from liquids containing melamine by biological means. The aqueous solution or suspension of melamine is brought into contact with microorganisms or enzyme preparations having melaminase activity, and the resulting mixture is maintained under anaerobic conditions whereby at least a portion of the melamine is biodegraded.
    Type: Grant
    Filed: March 19, 1979
    Date of Patent: January 27, 1981
    Assignee: Stamicarbon, B.V.
    Inventors: Lucia R. M. Meijer-Hoffman, Pieter H. de Jonge
  • Patent number: 4229544
    Abstract: A method of packaging, and the package so produced, for maintaining living organisms viable for a long period of time. The living organisms, such as bacteria, fungi, algae, etc., are mixed with a carrier, such as peat. The organism-carrier mixture is then disposed in a package, such as a heat-sealable plastic envelope, and a gaseous atmosphere is provided in the package effective to induce and maintain substantial nonvegatative state formation of the organisms. Some organisms will form cysts, others spores, but whatever nonvegetative state is assumed, the organisms will be much less susceptible to heat, cold, starvation, and other adverse environmental factors. Suitable gaseous atmospheres include nitrogen, helium, and argon gases. The package is then sealed to prevent contamination of the atmosphere therein.
    Type: Grant
    Filed: August 8, 1978
    Date of Patent: October 21, 1980
    Assignee: Payfer Laboratories Inc.
    Inventors: Robert W. Haynes, William H. Rasmussen
  • Patent number: 4166767
    Abstract: An insolubilized antibody suitable for an enzyme immuno assay or radio immuno assay, which has characteristic infrared absorptions at around 1,040 cm.sup.-1, 1,540 cm.sup.-1 and 1,640 cm.sup.-1 and is prepared by chemically binding an antibody to cell wall debris of bacteria or yeasts whose shape is globular or rod-like. There is further disclosed an enzyme immuno assay or radio immuno assay using the insolubilized antibody and a kit containing the insolubilized antibody.
    Type: Grant
    Filed: March 16, 1977
    Date of Patent: September 4, 1979
    Assignee: Dainippon Pharmaceutical Co., Ltd.
    Inventors: Shigeru Kurooka, Noriyuki Sunahara