Abstract: A D-aminoacid transaminase which converts CPC with .DELTA.-keto acids into .DELTA.-ketoadipinyl-7-ACA can be isolated from Bacillus Licheniformis ATCC 9945. This transamination can be applied to other D-amino acids and can also be used for the preparation of D-amino acids from .DELTA.-keto acids. The enzyme is also suitable for resolving racemates of D,L-amino acids and for detection of .DELTA.-keto acids alongside L-amino acids.

Abstract: This invention contemplates a novel process for the preparation of solution stable alpha-amylase obtained from Bacillus licheniformis, and to the high potency liquid enzyme product prepared by this process. Typically, the enzyme-containing solution from a fermentation is concentrated and starch is added to the concentrate. Alternatively, a precipitation agent such as salt is added to the solution, and a cake containing the enzyme precipitates. The cake is then contacted with an aqueous solution containing starch to extract the enzyme out of the cake to provide a stable liquid enzyme product.

Abstract: Disclosed is a method for enhancing the thermal stability of microbial alpha-amylase. The method involves adding a stabilizing amount of an amphiphile to the enzyme in its aqueous solution. Also included within the scope of the invention is the stabilized alpha-amylase formulation and its use in the liquefaction of starch.

Abstract: This invention relates to a novel process for the recovery of enzyme crystals. The enzymes may be obtained from any enzyme-producing microorganisms such as bacteria, fungi, and yeasts. The invention contemplates supersaturation and/or crystallization to obtain enzymes in the crystalline form, and is particularly effective for the recovery of heat stable alpha-amylase in a crystal form.

Abstract: A novel alpha-acetolactate decarboxylase enzyme product with improved stability is provided. The novel enzyme is produced in high levels by cultivation of Bacillus strains selected from the group consisting of Bacillus brevis and Bacillus licheniformis ATCC 11031, ATCC 12759, ATCC 12713, ATCC 11946, ATCC 27326, NRRL B-3751, NCTC 2120, NCTC 8721, NCIB 6816, NCIB 8537 and NCIB 11868.

Abstract: Improved production of .alpha.-amylase is realized by cultivating B. licheniformis ATCC No. 39326 in nutrient medium therefor, especially when medium contains lactose.

Abstract: Method of producing an egg white substitute material from soy protein. The method comprises extraction of a defatted soy bean material at a pH between about 6.0 and 10.5, separation, subjection of the supernatant to one or more ultrafiltrations and proteolytic hydrolysis of the supernatant or some fraction thereof to a DH between 1 and 8. The hydrolyzed soy material exhibits both a superior whipping or emulsifying ability and a good nutritional value, and it has no bitter taste.

Abstract: The presence of .beta.-lactam antibiotics in test material such as food, infusions, vaccines, blood for transfusion, body fluids, etc. may be determined by:seeding a nutrient medium with a .beta.-lactamase generating bacterium or spores thereof;applying a sample of said test material to a site on the so-called nutrient medium;then incubating the medium under conditions inducive to the generation of .beta.-lactamase by said bacteria; andassaying the .beta.-lactamase thus produced.

Abstract: A process for removing oleaginous materials containing those of animal origin from wastewater comprising treating wastewater containing oleaginous material with a microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ;and the microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2.

Abstract: A novel glucose isomerase enzyme useful for the conversion of glucose to fructose can be prepared by growing under aerobic conditions a culture of Bacillus licheniformis ATCC 31604 in a medium containing appropriate nutrients and then recovering the enyzme therefrom.

Abstract: A method for producing soy protein hydrolysate involving water washing fat-containing soy material at a pH of 3.5-5.5 thereby partially defatting the soy material; then hydrolyzing the partially defatted soy material with a proteolytic enzyme in the presence of water and a base to a DH in the range of 1-20; and recovering the aqueous soy protein hydrolysate from soy derived oil and solids in the hydrolysis mixture. The oil is recovered from the wash water and from the hydrolysate mixture.

Abstract: A process for removing oleaginous materials containing those of animal origin from wastewater comprising treating wastewater containing oleaginous material with a microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and the microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2.

Abstract: A novel protease product suitable for admixture to washing compositions and exhibiting substantially attenuated allergenic properties is prepared by cultivating strains of Bacillus licheniformis which have been mutated to block their synthesis of protease other than Subtilopeptidase A (subtilisin). The commercial Bacillus licheniformis derived protease products are mixtures of subtilisin and a non-serine protease of lower stability and greater allergenicity than subtilisin.

Abstract: Protease enzyme impurities contained in bacterial .alpha.-amylase enzyme preparations are inactivated by a mild heat treatment in the presence of a protective material. Useful protective materials include calcium and starch hydrolysates such as corn syrup. The protease-free .alpha.-amylase can then be used to solubilize starch materials by various granular starch and conventional processes. Hydrolysates obtained contain significantly less soluble protein than those prepared using untreated .alpha.-amylases. A preferred .alpha.-amylase enzyme preparation is one derived from a Bacillus licheniformis microorganism.

Abstract: A method for dissolving collagen-containing skin tissue by hydrolyzing said tissue in a first stage in the presence of urea with an alkaline proteinase having an activity optimum between pH 9 and 13, said hydrolysis proceeding in a hydrolysis medium which is initially in the pH region optimum for the enzyme employed, and optionally further hydrolyzing said tissue in at least one further stage by adding a weakly alkaline, neutral, or acid proteinase to said hydrolysis medium.