Corynebacterium Patents (Class 435/843)
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Patent number: 5304476Abstract: The present invention relates to a fermentation process using a L-lysine-producing microorganism having a resistance to an acyl-lysine, a methylated acyl-lysine or both an acyl-lysine and a methylated acyl-lysine. By the process of the present invention, L-lysine may be produced in high yields providing an improved process that reduces the cost of industrially produced L-lysine.Type: GrantFiled: March 3, 1992Date of Patent: April 19, 1994Assignee: Ajinomoto Co., Inc.Inventors: Manabu Yokomori, Kazuhiko Totsuka, Yoshio Kawahara, Harufumi Miwa, Tsuyoshi Osumi
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Patent number: 5294547Abstract: Culturing an L-amino acid producing microorganism belonging to the genus Brevibacterium or corynebacterium and having a resistance to a dipeptide containing glutamic acid or aspartic acid gives L-amino acids in high yield.Type: GrantFiled: September 6, 1991Date of Patent: March 15, 1994Assignee: Ajinomoto Company, Inc.Inventors: Takayasu Tsuchida, Haruo Uchibori, Hiroshi Takeuchi, Mitsuyoshi Seki
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Patent number: 5288620Abstract: Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid.Type: GrantFiled: May 20, 1992Date of Patent: February 22, 1994Assignee: Daicel Chemical Industries, Ltd.Inventors: Akinobu Matsuyama, Ichiro Takase, Yoichiro Ueda, Yoshinori Kobayashi
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Patent number: 5284757Abstract: L-arginine is produced in high yields by culturing a microorganism of the genus Brevibacterium or the genus Cornyebacterium, which is resistant to a compound of the formula X-guanidine, wherein X is an aliphatic group or derivative thereof. The preferred microorganisms are Brevibacterium flavum FERM BP-2227 and Corynebacterium glutamicum FERM BP-2228.Type: GrantFiled: April 23, 1993Date of Patent: February 8, 1994Assignee: Ajinomoto Company, Inc.Inventors: Takayasu Tsuchida, Noboro Ohtsuka, Hiroshi Takeuchi, Haruo Uchibori
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Patent number: 5279950Abstract: The present invention is directed to a process for the production of vanillin through the bioconversion of a vanillin precursor with a tissue culture of undifferentiated callus cells derived from a vanilla plant and/or enzymes obtained therefrom in the presence of a water soluble sulfhydryl compound and optionally, also in the presence of an assimilable carbon source.Type: GrantFiled: June 29, 1992Date of Patent: January 18, 1994Assignee: Kraft General Foods, Inc.Inventors: Ivica M. Labuda, Steven K. Goers, Kathleen A. Keon
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Patent number: 5272067Abstract: A process for the production of L-glutamic acid comprising growing microorganisms belonging to the genera Brevibacterium and Corynebacterium that are resisted to prumycin.Type: GrantFiled: February 4, 1992Date of Patent: December 21, 1993Assignee: Ajinomoto Co., Inc.Inventors: Takayasu Tsuchida, Mitsuyoshi Seki, Haruo Uchibori, Hiroki Kawashima, Hitoshi Enei
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Patent number: 5268293Abstract: Disclosed are Corynebacterium glutamicum CS-755 (KFCC 10672, FERM BP-2763), a L-lysine producing microorganism which is resistant to .alpha.-amino-.beta.-hydroxyvaleric acid, S-(.beta.-aminoethyl)-L-cysteine, methyl lysine, arginine analogues, other analogues, .beta.-(2 -thiazolyl)-DL-alanine, 5-hydroxyuridine, 6-azauracil, and 6-fluorotryptophan requires leucine and homoserine for growth, and a method for producing L-lysine comprising culturing the strain (KFCC 10672, FERM BP-2763) and recovering L-lysine from the resultant culture broth.Type: GrantFiled: March 16, 1992Date of Patent: December 7, 1993Assignee: Cheil Sugar Co., Ltd.Inventors: Jong W. Oh, Seong J. Kim, Young J. Cho, Nai H. Park, Jae H. Lee
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Patent number: 5264546Abstract: A process for the synthesis of copolymers comprising 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units by the cultivation of certain strains of Corynebacterium, and Rhodococcus, some of which are novel on a substrate containing e.g. glucose. The process can also be used to produce the previously unknown homopolymer of HV by using a substrate of valeric acid.Type: GrantFiled: April 17, 1992Date of Patent: November 23, 1993Assignee: Imperial Chemical Industries PLCInventors: Alistair J. Anderson, Edwin A. Dawes, Geoffrey W. Haywood, David Byrom
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Patent number: 5258305Abstract: The present invention provides a method of obtaining optically active 2-phenylpropionic acid and 2-phenylpropionamide such as S-(+)-2-phenylpropionic acid, R-(-)-2-phenylpropionamide, and R-(-)-2-phenylpropionic acid from S-2-phenylpropionitrile or R,S-2-phenylpropionamide using a bacterium belonging to Rhodococcus equi.Optically active 2-phenylpropionic acid and 2-phenylpropionamide are recovered in high yield and in quantities according to the method of the invention.Type: GrantFiled: March 12, 1992Date of Patent: November 2, 1993Assignees: Nitto Chemical Industry Co., Ltd., Lonza Japan, Ltd., Hideaki YamadaInventors: Hideaki Yamada, Toru Nagasawa
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Patent number: 5250423Abstract: L-lysine is produced by culturing, in a medium, an L-lysine-producing mutant which belongs to the genus Corynebacterium thermoaminogenes which is capable of growing at 40.degree. C. or higher and which has resistance to S-(2-aminoethyl)-L-cysteine thereby producing and accumulating L-lysine in the culture medium, and collecting L-lysine from the culture medium.Type: GrantFiled: April 22, 1991Date of Patent: October 5, 1993Assignee: Ajinomoto Co., Inc.Inventors: Yutaka Murakami, Harufumi Miwa, Shigeru Nakamori
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Patent number: 5250434Abstract: Novel bacteria identified as effective in glutamic acid production through fermentation chemistry conducted at temperatures above 42.degree. C.Type: GrantFiled: May 16, 1991Date of Patent: October 5, 1993Assignee: Ajinomoto Co., Inc.Inventors: Kazuhiko Yamada, Akira Seto
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Patent number: 5244803Abstract: There is disclosed a process for preparing optically active 3-phenylglycidic acid ester compound, which comprises permitting a culture broth, cells or treated cells of a microorganism having an ability of stereoselectively hydrolyzing a (2R, 3S)-3-phenylglycidic acid ester compound to act on a racemic 3-phenylglycidic acid ester compound which may also have a substituent on the phenyl group, thereby hydrolyzing the (2R, 3S) optically active isomer and separating and collecting the (2S, 3R) antipode from the reaction mixture.Type: GrantFiled: September 7, 1990Date of Patent: September 14, 1993Assignee: Tanabe Seiyaku Co., Ltd.Inventors: Takao Mori, Toshiyuki Furutani, Akio Nakao, Atsuhiko Tsujimura, Takeji Shibatani
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Patent number: 5240835Abstract: Unsaturated esters can be converted into unsaturated polymerizable monomers using a biocatalyst derived from Corynebacterium oxydans. The method involves the step of reacting an unsaturated ester with an organic compound having a primary or secondary hydroxy group in a substantially organic environment in the presence of the noted biocatalyst. A transacylase has been isolated from the microorganism and at least partially purified.Type: GrantFiled: October 10, 1989Date of Patent: August 31, 1993Assignee: Genencor International, Inc.Inventors: Frank A. Pettrone, Patrick J. Grisdale, Gregory M. Whited, Theresa C. Paulson
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Patent number: 5212077Abstract: The invention relates to a method for producing an organic compound from manure, including the steps of:i) concentrating the manure to form a vapor;ii) condensing said vapor to form a condensate;iii) adding to said condensate micro-organisms which are capable of producing the organic compound; andiv) separating from the condensate the organic compound produced by the micro-organisms.These micro-organisms comprise species of the genera Arthrobacter, Brevibacterium, Corynebacterium, Bacillus, Escherichia, Microbacterium, Micrococcus and Pseudomonas.Type: GrantFiled: April 20, 1990Date of Patent: May 18, 1993Assignee: MeMon B.V.Inventor: Erik D. J. Bleeker
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Patent number: 5210031Abstract: A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting an epihalohydrin with a halohydrin-halide-lyase originating from a microorganism selected from the group consisting of: Corynebacterium sp. N-2354 FERM BP-2726 and Microbacterium sp. N-4701 FERM BP-2644 in the presence of an alkali cyanide to thereby convert the epihalohydrin into the 4-halo-3-hydroxybutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.Type: GrantFiled: February 3, 1992Date of Patent: May 11, 1993Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Tetsuji Nakamura, Fujio Yu
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Patent number: 5200326Abstract: The invention is directed to a method of producing L-amino acids from .alpha.-ketocarboxylic acids by means of continuous fermentation with the aid of glutamate producing bacteria with biomass retention in which the cells are separated by microfiltration and/or centrifugal separators.Type: GrantFiled: May 1, 1987Date of Patent: April 6, 1993Assignee: Degussa AGInventors: Christian Wandrey, Rolf Wichmann, Ulrich Groeger, Manfred Kircher, Wolfgang Leuchtenberger, Eberhard Breuker
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Patent number: 5196326Abstract: A basic L-amino acid and an acidic L-amino acid may be concurrently produced by either culturing a basic L-amino acid-producing bacteria under conditions for producing an acidic L-amino acid or mix-culturing a basic L-amino acid-producing bacteria and an acidic L-amino acid-producing bacteria.Type: GrantFiled: February 15, 1991Date of Patent: March 23, 1993Assignee: Ajinomoto Co., Inc.Inventors: Hideo Kuronuma, Harufumi Miwa, Shigeru Nakamori, Toshimasa Ishii, Yasuhiko Yoshihara
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Patent number: 5194380Abstract: Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid.Type: GrantFiled: March 1, 1990Date of Patent: March 16, 1993Assignee: Daicel Chemical Industries, Ltd.Inventors: Akinobu Matsuyama, Ichiro Takase, Yoichiro Ueda, Yoshinori Kobayashi
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Patent number: 5188947Abstract: A process for producing L-ornithine by fermentation which comprises culturing a L-ornithine-producing microorganism is disclosed. The microorganism used belongs to the genus Brevibacterium, Corynebacterium, or Arthrobacter, has auxotrophy for arginine and/or citrulline, and has resistance to microphenolic acid and/or ornithinol.Type: GrantFiled: April 20, 1990Date of Patent: February 23, 1993Assignee: Ajinomoto Co., Inc.Inventors: Takayasu Tsuchida, Haruo Uchibori, Yoshitaka Nishimoto
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Patent number: 5188949Abstract: A method for producing L-threonine by fermentation which comprises culturing in a culture medium a microorganism of the genus Brevibacterium or Corynebacterium which is resistant to mycophenolic acid and is capable of producing L-threonine, accumulating L-threonine in the medium, and then recovering the L-threonine accumulated therein.Type: GrantFiled: August 22, 1990Date of Patent: February 23, 1993Assignee: Ajinomoto Co., Inc.Inventors: Takayasu Tsuchida, Naoki Katsurada, Noboru Ohtsuka, Haruo Uchibori, Takeshi Suzuki
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Patent number: 5188948Abstract: A process for producing L-valine by fermentation which comprises culturing in a liquid culture medium an L-valine producing microorganism which belongs to the genus Brevibacterium or Corynebacterium and which is resistant to a polyketide, and then recovering L-valine accumulated in said culture medium.Type: GrantFiled: February 9, 1990Date of Patent: February 23, 1993Assignee: Ajinomoto Co., Inc.Inventors: Naoki Katsurada, Haruo Uchibori, Takayasu Tsuchida
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Patent number: 5179010Abstract: A fermentative process for producing L-lysine is disclosed. The process is based on growing in a culture medium a mutant strain of the genus Brevibacterium or Corynebacterium (1) capable of producing L-lysine, and (2) having an intensified superoxide dismutase activity.Type: GrantFiled: January 26, 1990Date of Patent: January 12, 1993Assignee: Ajinomoto Co., Inc.Inventors: Yasuhiko Yoshihara, Yoshio Kawahara, Shigeho Ikeda
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Patent number: 5175108Abstract: The present invention relates to new plasmids isolated from Corynebacterium glutamicum, plasmid pGA1 and pGA2, which are compatible with one another. In addition, the present invention relates to recombinant plasmids and to plasmid vectors (shuttle vectors) containing the new plasmids. The plasmids and plasmid vectors of the present invention are suitable for use with genetic engineering to improve bacteria strains.Type: GrantFiled: August 27, 1991Date of Patent: December 29, 1992Assignee: Degussa AktiengesellschaftInventors: Frank Bachmann, Hans J. Kutzner, Hans Sonnen, Georg Thierbach, Petra-Sabine Kautz, Alfred Puhler, Andreas Schaefer
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Patent number: 5166061Abstract: A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting a 1,3-dihalo-2-propanol with a dehalogenating enzyme originating from a Corynebacterium or a Microbacterium in the presence of an alkali cyanide to thereby convert the 1,3-dihalo-2-propanol into the 4-halo-3-hydroyxbutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.Type: GrantFiled: July 20, 1990Date of Patent: November 24, 1992Assignee: Nitto Chemical Industry Co. Ltd.Inventors: Tetsuji Nakamura, Fujio Yu, Ichiro Watanabe
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Patent number: 5158891Abstract: A plasmid and a DNA fragment each of which contains a gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium, and a coryneform bacterium containing said DNA fragment. The gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium is a useful selective marker in effecting the breeding of a glutamic acid-producing coryneform bacterium by genetic recombination. Using the plasmid, the DNA fragment or the coryneform bacterium of the present invention, breeding of the glutamic acid-producing coryneform bacterium can be easily and effectively conducted by means of recombinant DNA technique.Type: GrantFiled: March 13, 1990Date of Patent: October 27, 1992Assignee: Asahi Kasei Kogyo Kabushiki KaishaInventors: Yasuhiko Takeda, Mikio Fujii, Yukihiro Nakajo, Sadao Isshiki
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Patent number: 5157207Abstract: A method for the production of a plant of reduced stature is provided comprising the introduction of a bacterial cell into a seed or a plant, the bacterial cell being capable of replicating in the plant and of inducing a reduction in plant stature. A seed and a plant modified by introduction of such a bacterial cell are also provided.Type: GrantFiled: February 6, 1990Date of Patent: October 20, 1992Assignee: Crop Genetics InternationalInventors: Peter S. Carlson, Jed W. Fahey, Jeffrey L. Flynn
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Patent number: 5155030Abstract: A process for preparing an optically active (R)-(-)-3-halo-1,2-propanediol comprising reacting an epihalohydrin with an epihalohydrin hydratase originating from a microorganism. For example, the microorganism belongs to the genus Corynebacterium or the genus Microbacterium. Epihalohydrins which can be used include epichlorohydrin and epibromohydrin. The reaction can be carried out at a temperature of from 5.degree. to 50.degree. C. and a pH of from 4 to 10.Type: GrantFiled: April 19, 1990Date of Patent: October 13, 1992Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Tetsuji Nakamura, Ichiro Watanabe
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Patent number: 5133976Abstract: A stable composition containing 35 to 48% lysine sulphate for animal nutrition is prepared by culturing a lysine-producing microorganism in a culture medium and adding sulfuric acid or ammonium sulphate during culturing to convert lysine as it is formed to lysine sulphate. The culture medium contains ammonia and ammonium sulphate, and a carbon source selected from glucose, sucrose and starch hydrolyzates in an amount such that the total weight ratio of fermentable substances/solids is greater than 80%. The concentration of the carbon source is maintained at 5 to 15 g/liter and ammonia is added during culturing to maintain an ammonium ion concentration of 1 to 5 g/liter. After allowing concentration of the carbon source to fall to less than 2 g/liter, culturing is stopped and the resultant cultured medium is concentrated and dried. The composition has good stability in moist air and preferably has a microporosity of about 0.3 cm.sup.3 /g and a surface area of about 0.5 m.sup.2 /g.Type: GrantFiled: February 28, 1991Date of Patent: July 28, 1992Assignee: Rhone-Poulenc SanteInventor: Noel Rouy
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Patent number: 5128253Abstract: The present invention is directed to a process for the production of vanillin through the bioconversion of a vanillin precursor with a ferulic acid degrading microorganism in the presence of a water soluble sulfhydryl compound and optionally, also in the presence of an assimilable carbon source.Type: GrantFiled: May 31, 1991Date of Patent: July 7, 1992Assignee: Kraft General Foods, Inc.Inventors: Ivica M. Labuda, Steven K. Goers, Kathleen A. Keon
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Patent number: 5126255Abstract: A process for the synthesis of copolymers comprising 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units by the cultivation of certain strains of Corynebacterium, and Rhodococcus, some of which are novel on a substrate containing e.g. glucose. The process can also be used to produce the previously unknown homopolymer of HV by using a substrate of valeric acid.Type: GrantFiled: May 1, 1990Date of Patent: June 30, 1992Assignee: Imperial Chemical Industries PLCInventors: Alistair J. Anderson, Edwin A. Dawes, Geoffrey W. Haywood, David Byrom
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Patent number: 5124257Abstract: A method for preparing L-alanine comprising culturing of mutants of microorganisms of the genus Brevibacterium or Corynebacterium requiring D-alanine for their growth on a nutrient medium containing assimilable sources of carbon, nitrogen, inorganic salts and stimulants of growth of the microorganisms until accumulation of L-alanine in the cultural liquid and the subsequent isolation of said product.Type: GrantFiled: December 22, 1989Date of Patent: June 23, 1992Inventors: Asmik G. Azizian, Artur A. Ambartsumian, Maritsa A. Ananikian, Shavarsh M. Kocharian
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Patent number: 5118619Abstract: A method for the fermentative production of L-isoleucine from D,L-.alpha.-hydroxybutyrate by means of mutants of the genus Corynbecaterium which utilize D-lactate.Type: GrantFiled: June 8, 1987Date of Patent: June 2, 1992Assignee: Degussa AktiengesellschaftInventors: Elisabeth Scheer, Hermann Sahm, Lothar Eggeling, Manfred Kircher, Wolfgang Leuchtenberger
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Patent number: 5084565Abstract: Nucleic acid probes capable of specifically hybridizing to rRNA of E. coli and Shigella species and not to rRNA of non-E. coli/Shigella are described along with methods utilizing such probes for the specific detection of E. coli and/or Shigella in food and other samples.Type: GrantFiled: August 18, 1988Date of Patent: January 28, 1992Assignee: Gene-Trak SystemsInventors: Kyriaki Parodos, Hsien-Yeh Hsu, David Sobell, Janice M. McCarty, David J. Lane
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Patent number: 5034318Abstract: A method for producing L-tryptophan comprising culturing coryneform glutamic acid-producing bacteria transformed with an expression vector capable of replicating and expressing an operably linked anthranilic acid phosphoribosyl transferase gene isolated from a brevibacterium glutamic acid-producing bacterium, growing the transformants in culture medium allowing for the production of L-tryptophan and recovering the L-tryptophan accumulated in the culture medium.Type: GrantFiled: March 22, 1990Date of Patent: July 23, 1991Assignee: Ajinomoto Co., Inc.Inventors: Kiyoshi Miwa, Shigeru Nakamori, Konosuke Sano
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Patent number: 5013656Abstract: A process is disclosed for producing orotic acid, the process involves culturing in a medium a microorganism of the genus Corynebacterium which is capable of producing orotic acid and has resistance to a pyrimidine analogue or to both a pyrimidine analogue and a sulfa drug, until orotic acid is accumulated in the culture, and recovering orotic acid therefrom.Type: GrantFiled: October 12, 1988Date of Patent: May 7, 1991Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kenichiro Takayama, Tomoko Matsunaga
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Patent number: 5008193Abstract: Methods for producing ascorbic acid using recombinant means comprising the transfer of genetic material by conjugation, a host cell lacking, entirely or to such an extent as not to be commercially useful, one or more enzymes in the metabolic path converting glucose to 2 keto-L-gulonic acid.Type: GrantFiled: May 21, 1987Date of Patent: April 16, 1991Assignee: Genentech, Inc.Inventors: Stephen Anderson, David R. Light, Cara Marks, William H. Rastetter
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Patent number: 4968609Abstract: The present invention relates to a process for producing an aromatic amino acid which comprises culturing a Coryneform bacterium carrying a recombinant DNA constructed by connecting (1) a gene capable of being expressed in a Coryneform bacterial cell and coding for 3-deoxy-D-arabino-heptulonic acid-7-phosphate synthetase with (2) a plasmid vector capable of propagating in a Coryneform bacterial cell.Type: GrantFiled: February 28, 1989Date of Patent: November 6, 1990Assignee: Ajinomoto Co., Inc.Inventors: Hisao Ito, Katsuaki Sato, Kazuhiko Matsui, Konosuke Sano, Shigeru Nakamori, Takashi Tanaka, Hitoshi Enei
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Patent number: 4954441Abstract: A process for producing L-lysine is disclosed in which a recombinant vector, a DNA fragment of which contains a gene involved in the synthesis of dihydropicolinic acid synthetase, is used to transform a microorganism of the genus Corynebacterium or Brevibacterium. The transformant is then cultured in a medium which supports the accumulation of L-lysine. Subsequently the L-lysine is recovered from the culture broth.Type: GrantFiled: May 25, 1989Date of Patent: September 4, 1990Assignee: Kyowa Hakko Kogyo Co. Ltd.Inventors: Ryoichi Katsumata, Toru Mizukami, Tetsuo Oka
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Patent number: 4946781Abstract: A recombinant DNA molecule comprising a plasmid vector having operationally inserted therein a gene coding for homoserine kinase is disclosed along with bacteria containing this recombinant DNA molecule and methods of using these bacteria to produce amino acids in large quantities.Type: GrantFiled: February 3, 1988Date of Patent: August 7, 1990Assignee: Ajinomoto Co., Inc.Inventors: Shigeru Nakamori, Hiroshi Takagi, Masaaki Ishida, Takaaki Sato, Kiyoshi Miwa, Konosuke Sano
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Patent number: 4931391Abstract: Nitrile hydration activity of the nitrilase or the immobilized from thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.Type: GrantFiled: May 1, 1987Date of Patent: June 5, 1990Assignees: Mitsubishi Rayon Kabushiki Kaisha, Nitto Kagaku Kogyo Kabushiki KaishaInventors: Kanehiko Enomoto, Koitchiro Ryuno, Hitoshi Shimizu
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Patent number: 4927753Abstract: A method is provided for the quantitative determination of L-fucose in a sample solution. According to the method an L-fucose dehydrogenase having its optimum pH around neutrality is allowed to act upon the sample solution in the presence or absence of an .alpha.-L-fucosidase, and the amount of reduced nicotinamide adenine dinucleotide thus formed is measured.Type: GrantFiled: January 14, 1987Date of Patent: May 22, 1990Assignee: Takara Shuzo Co., Ltd.Inventors: Takeshi Sakai, Susumu Matsui, Sumiko Akiyoshi, Akira Obayashi
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Patent number: 4927758Abstract: Disclosed is a process for producing histidine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of histidine and a vector DNA, culturing the transformant in a nutrient medium, accumulating histidine in the culture medium and recovering histidine therefrom.Type: GrantFiled: August 25, 1987Date of Patent: May 22, 1990Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Toru Mizukami, Ryoichi Katsumata, Tetsuo Oka
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Patent number: 4916067Abstract: Sorbic acid can be prepared by the activity of a specific microorganism on 2,4-hexadienal. This method can be performed under mild conditions and, different from organo-chemical methods, is advantageous because no by-products are formed.Type: GrantFiled: March 9, 1989Date of Patent: April 10, 1990Assignee: Idemitsu Kosan Company LimitedInventors: Motoshi Suzuki, Nobuo Murakami, Akira Inoue
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Patent number: 4908312Abstract: The invention relates to a bacterial process for producing phenylalanine. The process utilizes a Corynebacterium or Brevibacterium host which is transformed with a recombinant DNA. The recombinant DNA harbors a DNA containing a gene which encodes chorismate mutase or prephenate dehydratase. The transformed microorganism is then cultured in order to accumulate phenylalanine in the culture medium and the phenylalanine is recovered therefrom.Type: GrantFiled: December 7, 1988Date of Patent: March 13, 1990Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Ryoichi Katsumata, Tetsuo Oka
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Patent number: 4874698Abstract: Disclosed is a process for producing tryptophan by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of tryptophan and a vector DNA, culturing the transformant in a nutrient medium, accumulating tryptophan in the culture medium and recovering tryptophan therefrom.Type: GrantFiled: July 16, 1987Date of Patent: October 17, 1989Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Ryoichi Katsumata, Tetsuo Oka
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Patent number: 4871667Abstract: Muconic acid is obtained from the culture of strains of Arthrobacter sp. mutant strains, strains belonging to Corynebacterium acetoacidophilum, Corynebacterium lilium, genus Brevibacterium or genus Microbacterium.Type: GrantFiled: November 20, 1985Date of Patent: October 3, 1989Assignees: Agency of Industrial Science & Technology, Ministry of International Trade and Industry of JapanInventors: Yukio Imada, Nobuji Yoshikawa, Sumiko Mizuno, Takashi Mikawa
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Patent number: 4861722Abstract: A genetic sequence coding for the production of a protein having the activity of diaminopimelic acid decarboxylase and having two Pst I cleavage sites in its DNA chain and a molecular weight of 2.9.+-.0.05 Md, is incorporated into a vehicle capable of replication in Coryneform bacteria and used to produce L-lysine by fermentation.Type: GrantFiled: June 1, 1987Date of Patent: August 29, 1989Assignee: Ajinomoto Company, Inc.Inventors: Konosuke Sano, Koichi Ito, Kiyoshi Miwa, Shigeru Nakamori
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Patent number: 4851342Abstract: A method is described for producing acrylamide from acrylonitrile by the action of a microorganism having nitrilase activity in an aqueous medium, which comprises conducting the reaction in the presence of an alkali metal sulfate at an ionic activity of from 0.004 to 0.01 mole per liter while controlling the pH of said aqueous medium within a range of from 7 to 9 with an alkali hydroxide.Type: GrantFiled: January 18, 1985Date of Patent: July 25, 1989Assignees: Nitto Chemical Industry Co., Ltd., Mitsubishi Rayon Co., Ltd.Inventors: Ichiro Watanabe, Yasuo Ogawa, Susumu Seki
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Patent number: 4849354Abstract: Menaquinone-4 is produced by cultivating in culture media a microorganism belonging to the genus Corynebacterium Arthrobactor, Brevibacterium, Microbacterium, Cutobacterium, Auteobacterium or Flavobacterium which produces menaquinone-4 and recovering the same.Type: GrantFiled: May 14, 1986Date of Patent: July 18, 1989Assignee: Kyowa Hakko Kogyo Co., LtdInventors: Kenichiro Takayama, Yukoh Arai, Susumu Tomohiro, Shizuko Shirasuna
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Patent number: 4822738Abstract: A composite vector capable of the transduction of a Coryneform bacterium, which comprises a first DNA segment containing at least the genetic information for replication from a plasmid capable of propagating in a Coryneform bacterium and a second DNA segment containing at least a DNA region of a phage capable of propagating in the Coryneform bacterium, wherein the DNA region is incorporated into a particle of the aforementioned phage when the aforementioned bacterium is infected with the phage, is disclosed.Type: GrantFiled: December 21, 1987Date of Patent: April 18, 1989Assignee: Ajinomoto Co., Inc.Inventors: Kiyoshi Miwa, Konosuke Sano