Nocardia Patents (Class 435/872)
  • Publication number: 20040023396
    Abstract: A biosensor system includes a first optical waveguide optically coupled to a light source; a first ring or disk resonator optically coupled to the first optical waveguide; and a monitoring system that signals the presence of a biological material based on a detected change in one or more transfer characteristics of the first ring or disk resonator.
    Type: Application
    Filed: November 14, 2002
    Publication date: February 5, 2004
    Inventors: Robert W. Boyd, John E. Heebner
  • Patent number: 6365382
    Abstract: The very effective conversion of ML-236B substances and derivatives thereof into 6′-hydroxylated products with the microorganisms of species Amycolatopsis orientalis, or with an extract or a hydroxylation-effective enzyme derived from said microorganism, is described. The products obtained are suitable as HMG-CoA reductase inhibitors or intermediates thereof. Thus, the products can be used, for example, as an antihypercholesterolemic in pharmacy.
    Type: Grant
    Filed: December 27, 2000
    Date of Patent: April 2, 2002
    Assignee: LEK Pharmaceuticals and Chemical Company D.D.
    Inventors: Sa{haeck over (s)}o Kranjc, Irena Ivanc, Manica Schauer
  • Patent number: 6225438
    Abstract: This invention relates to polymer production and in particular to a novel copolymer and a process for microbiologically producing the same. More specifically this invention provides for a poly-3-hydroxyalkanoate (PHA) that includes medium length 3-hydroxyacyl monomers and a process comprising culturing a microorganism with a medium chain fatty carbon source and a fatty acid oxidation inhibitor. This invention allows the use of microorganisms which normally incorporate only short chain fatty acids to produce PHAs containing short and medium chain 3-hydroxyacyl monomers. The purpose of this invention is to produce a more versatile PHA polymer which includes C6, C7 and/or C8 3-hydroxyacyl monomers.
    Type: Grant
    Filed: January 31, 2000
    Date of Patent: May 1, 2001
    Assignee: The Procter & Gamble Company
    Inventor: Phillip Richard Green
  • Patent number: 6153419
    Abstract: The present invention relates to a method for quantitative determination of 1,5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of 1,5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1,5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture.
    Type: Grant
    Filed: October 16, 1997
    Date of Patent: November 28, 2000
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kazuo Aisaka, Sakae Tazoe, Katsuhiko Ando, Keiko Ochiai
  • Patent number: 6057147
    Abstract: The present invention is directed to an apparatus and method for enhanced bioremediation of hydrocarbons removed from a contaminated object comprising: (a) a basin for cleansing said hydrocarbon-contaminated object, said basin having a means for introducing a recycling bioremediating cleaning solution (NATURES WAY PC.TM.) for washing said object, a means for draining said solution from said basin into a biochamber reservoir and a means for screening particles from said solution upon entry into said reservoir; and (b) said reservoir having a means for temperature control between 90.degree. to 112.degree. F., means for aerating said solution, means for agitating said solution, an outlet means to a plurality of filters for filtering said solution, an inlet means from said filters and means for removing filtered sediments.
    Type: Grant
    Filed: January 21, 1997
    Date of Patent: May 2, 2000
    Inventors: Bert A. Overland, Gary M. Mitchell
  • Patent number: 6043064
    Abstract: An enzymatic hydroxylation process for the preparation of compounds useful as HMG-CoA reductase inhibitors and/or as intermediates in the preparation of HMG-CoA reductase inhibitors uses a microorganism or an enzyme derived from, or having the structure of an enzyme derived from, said microorganism, which is capable of catalyzing the hydroxylation process.
    Type: Grant
    Filed: October 22, 1993
    Date of Patent: March 28, 2000
    Assignee: Bristol-Myers Squibb Company
    Inventors: Brian L. Davis, Paul M. Cino, Laszlo Szarka
  • Patent number: 6004995
    Abstract: Compound 0406 having a chemical structural formula represented by the chemical formula (1) below and having an excellent immuno suppressive activity, and pharmaceutically acceptable salts thereof. ##STR1## (wherein R.sub.1 is --COCH.sub.2 COOH or H; R.sub.2 is --CO(CH.sub.2).sub.3 CH.sub.3 or --CO(CH.sub.2).sub.4 CH.sub.3 or H.
    Type: Grant
    Filed: April 24, 1997
    Date of Patent: December 21, 1999
    Assignee: Higeta Shoyu Co., LTD.
    Inventors: Yasushi Tanaka, Hisayuki Komaki, Yuzuru Mikami, Katsukiyo Yazawa, Jun'ichi Kobayashi
  • Patent number: 5981210
    Abstract: The method of the invention involves providing a first receptacle and a second receptacle. The first receptacle contains a sterile aqueous broth and the second receptacle contains an aqueous broth including a carbon source. The method then includes placing into the first receptacle a first support surface having a paraffin wax coating thereon and placing into the second receptacle a second support surface having a hydrophobic material coating thereon. A body specimen, such as sputum, is then introduced into each of the first and second receptacles. The presence of a nonparaffinophilic hydrophobic microorganism in the body specimen is determined by observing (i) a lack of microorganism growth on the paraffin coated material of the first support surface and (ii) a presence of microorganism growth on the hydrophobic material coating of the second support surface. The presence of the nonparaffinophilic hydrophobic microorganism can be further confirmed by performing a DNA extraction.
    Type: Grant
    Filed: July 24, 1998
    Date of Patent: November 9, 1999
    Assignee: Infectech, Inc.
    Inventor: Robert A. Ollar
  • Patent number: 5962304
    Abstract: The present invention provides a bacterial microorganism of the Rhodococcus or Nocardia species having the ability to degrade zearalenone.
    Type: Grant
    Filed: June 1, 1998
    Date of Patent: October 5, 1999
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Jon Duvick, Tracy A. Rood
  • Patent number: 5948746
    Abstract: The present invention relates to novel proteolytic enzymes. More specifically, the present invention relates to proteolytic enzymes obtainable from strains of Amycolata and Amycolatopsis. Moreover the invention relates to a process for the preparation of the proteolytic enzyme of the invention, as well as detergent additives and detergent compositions comprising the proteolytic enzyme.
    Type: Grant
    Filed: January 14, 1998
    Date of Patent: September 7, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Carsten Sj.o slashed.holm, Bjarne R.o slashed.nfeldt Nielsen, Claus Dambmann
  • Patent number: 5919672
    Abstract: A process for the microbiological or enzymatic hydrolytic resolution of racemic trans-2-(alkoxycarbonylethyl) lactams of the formula I: ##STR1## wherein R is C.sub.1 -C.sub.7 alkyl, 2,2,2-trifluoroethyl or methoxyethoxyethyl and R.sup.1 is hydrogen or a protecting group is disclosed, whereby an optically enriched compound of the formula Ib or IIa: ##STR2## is obtained.
    Type: Grant
    Filed: October 2, 1998
    Date of Patent: July 6, 1999
    Assignee: Schering Corporation
    Inventors: Michael J. Homann, William Brian Morgan
  • Patent number: 5854058
    Abstract: The present invention relates to a method of decomposing hard type rubber products, which comprises treating hard type rubber products with a microorganism belonging to the genus Nocardia in the presence of soft type rubber products. According to the present invention, hard type rubber products, particularly tires, can be efficiently decomposed.
    Type: Grant
    Filed: January 23, 1997
    Date of Patent: December 29, 1998
    Assignee: Director-General of Agency of Industrial Science and Technology
    Inventors: Akio Tsuchii, Kiyoshi Takeda, Yutaka Tokiwa
  • Patent number: 5846812
    Abstract: The present invention provides a bacterial microorganism having the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone. The present invention also provides a method for the isolation and utilization of a zearalenone-degradation gene encoding a gene product having the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone. In another embodiment, the present invention provides for the generation of transformants into which the zearalenone-degradation gene has been introduced, thereby providing the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone to said transformants. The present invention further provides a method for detoxification of plants pre- or post-harvest using microbes having the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone. The invention also provides a method for detoxification of plants pre- or post-harvest using the zearalenone-degradation gene.
    Type: Grant
    Filed: November 22, 1996
    Date of Patent: December 8, 1998
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Jon Duvick, Tracy A. Rood
  • Patent number: 5840551
    Abstract: A method of producing L-amino acids by fermentation. Microorganisms of the genus Corynebacterium which exhibit an auxotrophy relative to an amino acid are used as biocatalysts. The method is characterized in that the carbon source on the one hand and the limiting amino acid on the other hand are fed in two or more different infeed currents to the process. The infeed profiles have, for example, a concave (saccharose) and an exponential (amino acid) form or a convex (saccharose) and likewise a convex (amino acid) form, with specific differing degrees of increase of the currents relative to each other over time.
    Type: Grant
    Filed: December 19, 1996
    Date of Patent: November 24, 1998
    Assignee: Degussa Aktiengesellschaft
    Inventors: Holger Werning, Harald Voss, Walter Pfefferle, Wolfgang Leuchtenberger
  • Patent number: 5756326
    Abstract: A method is disclosed for the high efficiency transformation of species of the genus Nocardia with DNA molecules. DNA vectors for the transformation of genes into Nocardia as well as recombinant Nocardia host cells expressing recombinant genes are disclosed.
    Type: Grant
    Filed: July 7, 1997
    Date of Patent: May 26, 1998
    Assignee: Merck & Co., Inc.
    Inventors: Juan F. Martin, Juan-Jose R. Coque, C. Vasant Kumar
  • Patent number: 5714364
    Abstract: A method for producing in culture phenylalanine dehydrogenase at yields of 50+ units of enzyme per litre of culture, one unit measured as the amount required to convert one micro-mole of phenylalanine to phenyl pyrovic acid at 25.degree. C. and pH 10.8, in a growth medium comprising a nitrogen source, a buffer, phenylalanine or phenylpyruvic acid or a salt thereof at 20 mM and optionally a vitamin solution. Using phenylalanine at about 100 mM gives yields of phenylalanine dehydrogenase enzyme of 350+ units per liter of culture.
    Type: Grant
    Filed: March 20, 1996
    Date of Patent: February 3, 1998
    Assignee: Microbiological Research Authority
    Inventors: Peter Michael Hammond, Graham Mark Brearley, Christopher Philip Price
  • Patent number: 5622847
    Abstract: Solid materials are separated from micro organisms by a process which comprises growing them at a fermentation temperature, conditioning them by cooling them without freezing and then heating to above the fermentation temperature without permitting substantial recovery from the conditioning stage.
    Type: Grant
    Filed: May 12, 1995
    Date of Patent: April 22, 1997
    Assignee: Zeneca Limited
    Inventors: Robert H. Cumming, Julie S. Watson, Paul Rees
  • Patent number: 5527697
    Abstract: An esterase with a molecular weight of 55,200 .+-.300 Da is suitable for the chemoselective conversion of a compound of the formula II into a compound of the formula I.
    Type: Grant
    Filed: July 12, 1994
    Date of Patent: June 18, 1996
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Werner Aretz, Klaus Sauber
  • Patent number: 5516676
    Abstract: Compounds useful as intermediates in the preparation of taxanes such as taxol are prepared by contacting one or more C-13 acyloxy-bearing taxanes with an enzyme or microorganism capable of hydrolyzing said acyloxy groups to hydroxyl groups. In a preferred embodiment the microorganism is Nocardioides albus ATCC55424, Nocardioides albus ATCC55425 or Nocardioides luteus ATCC55426 or the hydrolase enzyme is isolated from the microorganisms.
    Type: Grant
    Filed: June 15, 1993
    Date of Patent: May 14, 1996
    Assignee: Bristol-Myers Squibb Company
    Inventors: Ronald L. Hanson, Ramesh N. Patel, Laszlo J. Szarka
  • Patent number: 5434062
    Abstract: The present invention relates to a type of polymer comprising repeating units of formula ##STR1## and in particular to a poly-.beta.-hydroxybutyrate polyester type polymer. The present invention also relates to a microbiological process for the production of the above type of polymer, to a methylotrophic microorganism (bacterium) for use in such a process, and to means for controlling such bioprocesses.
    Type: Grant
    Filed: January 21, 1994
    Date of Patent: July 18, 1995
    Assignee: National Research Council of Canada
    Inventors: Denis Groleau, Denis Bourque, Yves Pomerleau
  • Patent number: 5420023
    Abstract: A process for producing L-phenylalanine at high rates of formation and in good yields is provided which comprises allowing a microorganism which belongs to the genus Nocardia and is capable of producing L-phenyl-alanine from phenylpyruvic acid or a salt thereof and an amino group donor to act upon an aqueous solution containing phenylpyruvic acid or a salt thereof and an amino group donor selected from the group consisting of ammonium salts and ammonia in a hydrogen gas atmosphere.
    Type: Grant
    Filed: January 11, 1993
    Date of Patent: May 30, 1995
    Assignee: Kuraray Co., Ltd.
    Inventors: Tadashi Matsunaga, Takanori Kitamura
  • Patent number: 5391495
    Abstract: The present invention concerns a process for the stereoselective enzymatic reduction of keto group-containing compounds such as N-(4-(2-chloroacetyl)phenyl)methanesulfonamido to form the corresponding hydroxyl-group containing compound. The process is selective for the D(+) enantiomer and is catalyzed by enzymes such as oxido-reductase or dehydrogense, or by microorganisms such as Hansenula, Rhodococcus, or Norcardia species.
    Type: Grant
    Filed: October 27, 1993
    Date of Patent: February 21, 1995
    Assignee: Bristol-Myers Squibb Company
    Inventors: Ramesh N. Patel, Amit Banerjee, Clyde G. McNamee, Laszlo J. Szarka
  • Patent number: 5387513
    Abstract: A microbiological process, capable of producing polymers comprising monomer repeat units of the structure--O.CH((CH.sub.2).sub.x CH.sub.3).CH.sub.2.--CO--wherein x is particularly 6 from glucose, novel polymers produced through the process and new strains of Pseudomonas for use in the process.
    Type: Grant
    Filed: December 16, 1993
    Date of Patent: February 7, 1995
    Assignee: Zeneca Limited
    Inventors: Alistair J. Anderson, Edwin A. Dawes, Geoffrey W. Haywood, David Byrom
  • Patent number: 5324662
    Abstract: Microorganisms or reductases derived therefrom reduce a diketo ester ##STR1## to form the associated 3-hydroxy, 5-hydroxy, or 3,5-dihydroxy esters.
    Type: Grant
    Filed: May 15, 1992
    Date of Patent: June 28, 1994
    Assignee: E. R. Squibb & Sons, Inc.
    Inventors: Ramesh N. Patel, Clyde G. McNamee, Amit Banerjee, Laszlo J. Szarka
  • Patent number: 5312738
    Abstract: New glycopeptide antibiotic A82846, comprising A82846A, A82846B and A82846C, is produced by Nocardia orientalis strains NRRL 18098, NRRL 18099 and NRRL 18100. The A82846 antibiotics have activity against Gram-positive bacteria comparable to that of vancomycin.
    Type: Grant
    Filed: May 9, 1990
    Date of Patent: May 17, 1994
    Assignee: Eli Lilly and Company
    Inventors: Robert L. Hamill, James A. Mabe, David F. Mahoney, Walter M. Nakatsukasa, Raymond C. Yao
  • Patent number: 5275936
    Abstract: A process is claimed for the preparation of 1-methyl-1,4-androstadiene-3,17-dione characterized by fermenting 1-methyl-5.alpha.-androst-1-ene-3,17-dione (metenolone) with a microorganism culture of the genera Nocardia, Mycobacterium, or Fusarium.
    Type: Grant
    Filed: April 6, 1992
    Date of Patent: January 4, 1994
    Assignee: Schering Aktiengesellschaft
    Inventor: Karl Petzoldt
  • Patent number: 5264546
    Abstract: A process for the synthesis of copolymers comprising 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units by the cultivation of certain strains of Corynebacterium, and Rhodococcus, some of which are novel on a substrate containing e.g. glucose. The process can also be used to produce the previously unknown homopolymer of HV by using a substrate of valeric acid.
    Type: Grant
    Filed: April 17, 1992
    Date of Patent: November 23, 1993
    Assignee: Imperial Chemical Industries PLC
    Inventors: Alistair J. Anderson, Edwin A. Dawes, Geoffrey W. Haywood, David Byrom
  • Patent number: 5246841
    Abstract: A process for the production of eicosapentaenoic acid comprising the steps of a) culturing a microorganism capable of producing lipid containing eicosapentaenoic acid belonging to a genus selected from the group of Pseudomonas, Alteromonas and Shewanella, to produce lipid containing eicosapentaenoic acid, and b) recovering the eicosapentaenoic acid from the cultured product; a process for production of a lipid containing eicosapentaenoic acid comprising the steps of a) culturing a microorganism capable of producing lipid containing eicosapentaenoic acid belonging to a genus selected from the group of Pseudomonas, Alteromonas and Shewanella, to produce lipid containing eicosapentaenoic acid, and b) recovering the lipid containing eicosapentaenoic acid; and microorganisms capable of producing eicosapentaenoic acid belonging to a genus selected from the group consisting of Pseudomonas, Alteromonas, and Shewanella.
    Type: Grant
    Filed: October 25, 1991
    Date of Patent: September 21, 1993
    Assignee: Sagami Chemical Research Center
    Inventors: Kazunaga Yazawa, Keiko Araki, Noriko Okazaki, Naganori Numao, Kiyosi Kondo
  • Patent number: 5242820
    Abstract: The invention relates to a novel pathogenic mycoplasma isolated from patients with Acquired Immune Deficiency Syndrome (AIDS) and its use in detecting antibodies in sera of AIDS patients, patients with AIDS-related complex (ARC) or patients dying of diseases and symptoms resembling AIDS diseases. The invention further relates to specific DNA sequences, antibodies against the pathogenic mycoplasma, and their use in detecting DNA or antigens of the pathogenic mycoplasma or other genetically and serologically closely related mycoplasmas in infected tissue of patients with AIDS or ARC or patients dying of symptoms resembling AIDS diseases. The invention still further relates to a variety of different forms of vaccine against mycoplasma infection in humans and/or animals.
    Type: Grant
    Filed: June 6, 1991
    Date of Patent: September 7, 1993
    Assignee: American Registry of Pathology
    Inventor: Shyh-Ching Lo
  • Patent number: 5239064
    Abstract: A novel antibiotic complex, A59770, individual factors and derivatives thereof, are provided. The A59770 compounds are useful as pesticides, particularly as rodenticides. Further provided are methods for reducing a rodent population, rodenticidal compositions, and an A59770-producing Amycolatopsis orientalis culture.
    Type: Grant
    Filed: April 11, 1990
    Date of Patent: August 24, 1993
    Assignee: Eli Lilly and Company
    Inventors: Marvin M. Hoehn, Karl H. Michel, Raymond C. Yao
  • Patent number: 5223416
    Abstract: A process for the predominantly producing R(-)-mandelic acid or a derivative thereof which comprises subjecting (i) R,S-mandelonitrile or a derivative thereof, or (ii) a mixture of prussic acid and benzaldehyde or a derivative of benzaldehyde to the action of a microorganism selected from the group consisting of the genus Aureobacterium, Pseudomonas, Caseobacter, Alcaligenes, Acinetobacter, Brevibacterium, Nocardia, and Bacillus or treated cells thereof, which the microorganism is capable of stereospecifically hydrolyzing a nitrile group of the R,S-mandelonitrile or a derivative thereof, in a neutral or basic aqueous reaction system to produce the R(-)-mandelic acid.
    Type: Grant
    Filed: March 29, 1991
    Date of Patent: June 29, 1993
    Assignee: Nitto Chemical Industry Co., Ltd.
    Inventors: Takakazu Endo, Koji Tamura
  • Patent number: 5206158
    Abstract: The invention provides a process for the preparation of 2,6-difluorobenzamide which comprises subjecting 2,6-difluorobenzonitrile to the action of a suitable hydrolysing microorganism or nitrilase extract therefrom; and Rhodococcus Sp. NCIB 12218 or a mutant thereof, suitable for use in the process.
    Type: Grant
    Filed: August 21, 1990
    Date of Patent: April 27, 1993
    Assignees: Gist-Brocades N.V., Shell Internationale Research Maatschappij B.V.
    Inventors: Kenneth H. Clifford, Philip J. Geary, Robert J. Pryce
  • Patent number: 5192742
    Abstract: New glycopeptide antibiotics MM 49728, MM 55266, MM 55267/1, MM 55267/2 and MM 55268 are produced by fermentation of Amycolatopsis sp. NCIB 40089.
    Type: Grant
    Filed: December 18, 1991
    Date of Patent: March 9, 1993
    Assignee: Beecham Group PLC
    Inventors: Nigel J. Coates, Rachel Sykes, Christopher J. Davis, Lawrence M. Curtis
  • Patent number: 5187088
    Abstract: A novel choline oxidase being thermostable at 50.degree. C. at pH 7 to 9 is disclosed. A method for producing the enzyme by using actinomycetes is also disclosed.
    Type: Grant
    Filed: September 5, 1991
    Date of Patent: February 16, 1993
    Assignee: Takeda Chemical Industries, Ltd.
    Inventors: Itsuo Furuoya, Takashi Suzuki, Takeshi Takahashi
  • Patent number: 5179014
    Abstract: A process for the production of amides utilizing microorganisms is described, which comprises subjecting nitriles having from 2 to 6 carbon atoms to the action of a microorganism belonging to the genus Rhodococcus, and genus Arthrobacter or the genus Microbacterium having an ability to hydrate the nitriles to form the corresponding amides in an aqueous medium.
    Type: Grant
    Filed: May 14, 1990
    Date of Patent: January 12, 1993
    Assignee: Nitto Chemical Industry Co., Ltd.
    Inventors: Ichiro Watanabe, Masami Okumura
  • Patent number: 5153134
    Abstract: A method for the fermentation of microorganisms having a high level of ice nucleating activity is disclosed. A high productivity in the fermentation is achieved by using a certain amount of nitrogen source during the growth phase and low temperature during the stationary phase of the fermentation.
    Type: Grant
    Filed: March 5, 1987
    Date of Patent: October 6, 1992
    Assignee: Genencor International, Inc.
    Inventors: Richard J. Lawless, Jr., Richard J. LaDuca
  • Patent number: 5143841
    Abstract: This invention provides a novel ganglioside ceramidase which has a substrate specificity at least for GD1a, GM1, GM2 and GM3 and acts at least on GD1a, GM1, GM2 and GM3 and catalyzes the reaction of hydrolysis of ganglioside to lysoganglioside and fatty acid.This invention further provides a process for producing the novel ganglioside ceramidase which comprises cultivating ganglioside ceramidase producing strain belonging to the genus Nocardia in a culture medium and collecting ganglioside ceramidase from the culture.
    Type: Grant
    Filed: September 12, 1990
    Date of Patent: September 1, 1992
    Assignee: Toyo Jozo Company, Ltd.
    Inventors: Yoshio Hirabayashi, Tatsurokuro Tochikura, Setsu Kadowaki, Kenji Yamamoto
  • Patent number: 5135857
    Abstract: The object of this invention are de-mannosylated teicoplanin derivatives which can be obtained in good yield by microbiological transformation with cultures of Nocardia orientalis NRRL 2450 or Streptomyces candidus NRRL 3218.
    Type: Grant
    Filed: July 12, 1991
    Date of Patent: August 4, 1992
    Assignee: Gruppo Lepetit S.p.A.
    Inventors: Angelo Borghi, Giancarlo Lancini, Piero Antonini
  • Patent number: 5126255
    Abstract: A process for the synthesis of copolymers comprising 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units by the cultivation of certain strains of Corynebacterium, and Rhodococcus, some of which are novel on a substrate containing e.g. glucose. The process can also be used to produce the previously unknown homopolymer of HV by using a substrate of valeric acid.
    Type: Grant
    Filed: May 1, 1990
    Date of Patent: June 30, 1992
    Assignee: Imperial Chemical Industries PLC
    Inventors: Alistair J. Anderson, Edwin A. Dawes, Geoffrey W. Haywood, David Byrom
  • Patent number: 5071752
    Abstract: Process is described for the production of L-amino acids of general formula I ##STR1## in which R.sub.1 means an alkyl radical with at most 12 carbon atoms optionally substituted by hydroxy groups, mercapto groups, halogen atoms, amino groups, carbonyl groups or guanidino groups and/or interrupted by oxygen atoms, nitrogen atoms or sulfur atoms, and in the case of mercapto compounds of formula I also their dithio compounds, characterized in that the microorganism Nocardia spec. DSM 3306 or its enzymes are allowed to act on a D,L-imidazolidinedione derivative of general formula II ##STR2## in which R.sub.1 has the above-named meaning or, in the case of mercapto compounds of formula II, also in their dithio compounds.
    Type: Grant
    Filed: September 23, 1988
    Date of Patent: December 10, 1991
    Assignee: Schering Aktiengesellschaft
    Inventors: Uwe Klages, Alfred Weber, Ludwig Wilschowitz
  • Patent number: 5059532
    Abstract: Disclosed herein is a process for producing an unsaturated fatty acid or derivative thereof which comprises culturing an unsaturated fatty acid-producing microorganism belonging to the genus Rhodococcus in a medium containing a saturated fatty acid or a derivative thereof, or causing the resting cells of the microorganisms to act on a saturated fatty acid or a derivative thereof. Also disclosed herein is a process for producing an alkene or derivative thereof which comprises culturing an unsaturated hydrocarbon compound-producing microorganism belonging to the genus Rhodococcus in a medium containing an alkane or derivative thereof, or causing the resting cells of the microorganism to act on an alkane or derivative thereof.
    Type: Grant
    Filed: December 1, 1987
    Date of Patent: October 22, 1991
    Assignee: Agency of Industrial Science & Technology
    Inventors: Yoshiharu Kimura, Shigehito Adachi, Katsutoshi Ara, Shigeo Inoue, Kenzo Koike
  • Patent number: 5043035
    Abstract: In a method of disassembling resin-molded equipment, a gap is formed in the boundary portion between a metal body and a resin layer disposed around the metal body in order to separate the metal body is separated from the resin layer. To form the gap, either the metal body or the resin layer may be heated, so that the gap is formed by the difference in thermal expansion between the metal body and the resin layer. Alternatively, a microorganism such as ray fungus of Nocardia is attached to the boundary portion between the metal body and the resin layer, and decomposes the resin to form the gap. Since the gap reduces the bonding strength between the metal body and the resin layer and in some cases the metal body may even separate from the resin layer, the metal body may be pulled out with ease.
    Type: Grant
    Filed: January 11, 1991
    Date of Patent: August 27, 1991
    Assignee: Kabushiki Kaisha Toshiba
    Inventor: Tetsuji Yoshikawa
  • Patent number: 5021234
    Abstract: An agent for the diagnosis and treatment of malignant tumors and for the treatment of lowered cellular and humoral immune defense is described which contains an immune modulator, a lipopolysaccharide or an immune modulator tagged with a radioactive tracer, a dyestuff or a cytostatic, or a similar polysaccharide, possibly in and/or on liposomes or lipidized. There is also described a product which contains the agent together with an adjuvant consisting of an aldehyde and an alcohol. There is also described a method for the diagnosis of malignant tumors comprising the step of administering to a mammal a diagnostic effective amount of the agent, optionally together with an adjuvant consisting of an aldehyde and an alcohol.
    Type: Grant
    Filed: February 26, 1990
    Date of Patent: June 4, 1991
    Inventor: Udo Ehrenfeld
  • Patent number: 4997755
    Abstract: A microbiological process is described for the preparation of 6-desmethyl-6-.beta.-hydroxymethyl and 6-desmethyl-6-.beta.-carboxylovastatin derivatives and 8-acyl analogs thereof.
    Type: Grant
    Filed: April 14, 1988
    Date of Patent: March 5, 1991
    Assignee: Merck & Co., Inc.
    Inventors: Joanne M. Williamson, David Houck, Edward S. Inamine
  • Patent number: 4997760
    Abstract: This invention provides a novel ganglioside ceramidase which has a substrate specificity at least for GD1a, GM1, GM2 and GM3 and acts at least on GD1a, GM1, GM2 and GM3 and catalyzes the reaction of hydrolysis of ganglioside to lysoganglioside and fatty acid.This invention further provides a process for producing the novel ganglioside ceramidase which comprises cultivating ganglioside ceramidase producing strain belonging to the genus Nocardia in a culture medium and collecting ganglioside ceramidase from the culture.
    Type: Grant
    Filed: August 9, 1988
    Date of Patent: March 5, 1991
    Assignee: Toyo Jozo Company, Ltd.
    Inventors: Yoshio Hirabayashi, Tatsurokuro Tochikura, Setsu Kadowaki, Kenji Yamamoto
  • Patent number: 4956284
    Abstract: A process for the preparation of metoprolol in a stereospecific form or a non-toxic, pharmaceutically acceptable acid addition salt thereof and/or a stereospecific form of 4-(2-methoxyethyl)-phenyl glycidyl ether which comprises subjecting 4-(2-methoxyethyl)-phenyl allyl ether to the action of a microorganism having the ability for stereoselective epoxidation of 4-(2-methoxyethyl)-phenyl allyl ether into 4-(2-methoxyethyl)-phenyl glycidyl ether having at least 80% by weight the S configuration, at least partly separating 4-(2-methoxyethyl)-phenyl glycidyl ether and/or reacting 4-(2-methoxyethyl)-phenyl glycidyl ether with isopropylamine and at least partly separating metoprolol and/or converting metoprolol into its non-toxic, pharmaceutically acceptable acid addition salt.
    Type: Grant
    Filed: February 6, 1986
    Date of Patent: September 11, 1990
    Assignee: Gist-Brocades N.V.
    Inventors: Gareth T. Phillips, Brian W. Robertson, Mauro A. Bertola, Hein S. Koger, Arthur F. Marx, Peter D. Watts
  • Patent number: 4956293
    Abstract: A novel antibiotic, A 42867, is prepared by cultivating the strain Nocardia sp. ATCC 53492, or an antibiotic A 42867-producing mutant or variant thereof, under submerged aerobic conditions in the presence of assimilable sources of carbon, nitrogen and inorganic salts. The antibiotic and its addition salts are useful in the treatment of infectious diseases and as growth promotant agents.
    Type: Grant
    Filed: November 14, 1988
    Date of Patent: September 11, 1990
    Assignee: Gruppo Lepetit S.p.A.
    Inventors: Ernesto Riva, Maurizio Denaro, Enrico Selva, Giovanni Cassani, Francesco Parenti
  • Patent number: 4952500
    Abstract: A plasmid transformation system for Rhodococcus was developed using an Escherichia coli-Rhodococcus shuttle plasmid. Rhodococcus sp. H13-A contains three cryptic indigenous plasmids, designated pMVS100, pMVS200 and pMVS300, of 75, 19.5 and 13.4 kilobases (Kb), respectively. A 3.8 Kb restriction fragment of pMVS300 was cloned into pIJ30, a 6.3 Kb pBR322 derivative, containing the E. coli origin of replication (ori) and ampicillin resistance determinant (bla) as well as a Streptomyces gene for thiostrepton resistance, tsr. The resulting 10.1 Kb recombinant plasmid, designated pMVS301, was isolated from E. coli DH1 (pMVS301) and transformed into Rhodococcus sp. AS-50, a derivative of strain H13-A, by polyethylene glycol-assisted transformation of Rhodococcus protoplasts and selection for thiostrepton-resistant transformants. This strain was deposited with the ATCC on Feb. 1, 1988 and assigned ATCC 53719.The plasmid contains the Rhodococcus origin of replication.
    Type: Grant
    Filed: February 1, 1988
    Date of Patent: August 28, 1990
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: William R. Finnerty, Mary E. Singer
  • Patent number: 4931391
    Abstract: Nitrile hydration activity of the nitrilase or the immobilized from thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.
    Type: Grant
    Filed: May 1, 1987
    Date of Patent: June 5, 1990
    Assignees: Mitsubishi Rayon Kabushiki Kaisha, Nitto Kagaku Kogyo Kabushiki Kaisha
    Inventors: Kanehiko Enomoto, Koitchiro Ryuno, Hitoshi Shimizu
  • Patent number: 4920215
    Abstract: The antibiotic of the formula: ##STR1## wherein R is ##STR2## The antibiotic is useful for the treatment of swine dysentery and for promoting growth and/or increasing the efficiency of food utilization in poultry, swine and ruminants.
    Type: Grant
    Filed: December 16, 1987
    Date of Patent: April 24, 1990
    Assignee: Pfizer Inc.
    Inventors: Kelvin S. Holdom, John C. Ruddock, Junsuke Tone, Hiroshi Maeda, Martin R. Jefson