Salmonella Patents (Class 435/879)
  • Patent number: 7919081
    Abstract: The present disclosure describes pathogenic bacteria that have been modified to be deficient in NiFe hydrogenase activity; compositions comprising such modified bacteria, and the use of such bacteria to protect animals from pathogenic enteric bacterial infections.
    Type: Grant
    Filed: February 28, 2005
    Date of Patent: April 5, 2011
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Robert J. Maier, John S. Gunn
  • Patent number: 7842496
    Abstract: Selective growth media N4 agars that include a combination of high levels of yeast extract, possibly higher levels of protein, elevated levels of sugar and reduced levels of sodium chloride. The combination of ingredients provides the ability to detect Salmonella spp. and Shigella spp. with almost identical sensitivity while alleviating false-negative and false-positive problems commonly encountered with the presence of Proteus spp. and Citrobacter spp.
    Type: Grant
    Filed: May 2, 2007
    Date of Patent: November 30, 2010
    Assignee: University of Maryland
    Inventors: Russell G. Miller, Edward T. Mallinson
  • Patent number: 7354592
    Abstract: The present invention is directed to mutant Salmonella sp. having a genetically modified msbB gene in which the mutant Salmonella is capable of targeting solid tumors. The invention is also directed to Salmonella sp. containing a genetically modified msbB gene as well as an genetic modification in a biosynthetic pathway gene such as the purl gene. The present invention further relates to the therapeutic use of the mutant Salmonella for growth inhibition and/or reduction in volume of solid tumors.
    Type: Grant
    Filed: February 23, 2005
    Date of Patent: April 8, 2008
    Assignees: Vion Pharmaceuticals, Inc., Yale University
    Inventors: David Bermudes, Kenneth Brooks Low
  • Patent number: 7208286
    Abstract: Monolithic bioelectronic devices for the detection of ammonia includes a microorganism that metabolizes ammonia and which harbors a lux gene fused with a heterologous promoter gene stably incorporated into the chromosome of the microorganism and an Optical Application Specific Integrated Circuit (OASIC). The microorganism is generally a bacterium.
    Type: Grant
    Filed: June 14, 2005
    Date of Patent: April 24, 2007
    Assignee: UT-Battelle LLC
    Inventors: Michael L. Simpson, Michael J. Paulus, Gary S. Sayler, Bruce M. Applegate, Steven A. Ripp
  • Patent number: 7083794
    Abstract: Attenuated immunogenic bacteria having an RpoS+ phenotype, in particular, Salmonella enterica serotype Typhi having an RpoS+ phenotype and methods therefor are disclosed. The Salmonella have in addition to an RpoS+ phenotype, an inactivating mutation in one or more genes which render the microbe attenuated, and a recombinant gene capable of expressing a desired protein. The Salmonella are attenuated and have high immunogenicity so that they can be used in vaccines and as delivery vehicles for genes and gene products. Also disclosed are methods for preparing the vaccine delivery vehicles.
    Type: Grant
    Filed: May 3, 2002
    Date of Patent: August 1, 2006
    Assignee: Washington University
    Inventors: Roy Curtiss, III, Cheryl A. Nickerson
  • Patent number: 6923972
    Abstract: The present invention is directed to mutant Salmonella sp. having a genetically modified msbB gene in which the mutant Salmonella is capable of targeting solid tumors. The present invention further relates to the therapeutic use of the mutant Salmonella for growth inhibition and/or reduction in volume of solid tumors.
    Type: Grant
    Filed: April 18, 2002
    Date of Patent: August 2, 2005
    Assignees: Vion Pharmaceuticals, Inc., Yale University
    Inventors: David Bermudes, Kenneth Brooks Low
  • Patent number: 6863894
    Abstract: The present invention is directed to mutant Salmonella sp. having a genetically modified msbB gene in which the mutant Salmonella is capable of targeting solid tumors. The invention is also directed to Salmonella sp. containing a genetically modified msbB gene as well as an genetic modification in a biosynthetic pathway gene such as the purI gene. The present invention further relates to the therapeutic use of the mutant Salmonella for growth inhibition and/or reduction in volume of solid tumors.
    Type: Grant
    Filed: June 27, 2002
    Date of Patent: March 8, 2005
    Assignees: Vion Pharmaceuticals, Inc., Yale University
    Inventors: David Bermudes, Kenneth Brooks Low
  • Patent number: 6649398
    Abstract: A bacterial autoinducer and method for isolating and purifying a bacterial autoinducer form a sample comprising the steps of collecting a sample containing the autoinducer, fractionating the sample to isolate fractions corresponding to molecular weights of approximately 300-1500 Dalton, and eluting the isolate on an anion-exchange chromatographic column and selecting the faction containing the autoinducer.
    Type: Grant
    Filed: July 12, 2001
    Date of Patent: November 18, 2003
    Assignee: University of Leicester
    Inventors: Primrose Pamela Elaine Freestone, Peter Humphrey Williams, Mark Lyte, Richard David Haigh
  • Patent number: 6610531
    Abstract: A method is provided for preserving live bacteria by subjecting an aqueous system containing the growing bacteria to drying without special equipment, in the presence of trehalose with or without the addition of divalent cations as stabilizing agents. Further, a dried composition for preservation of aerobic bacteria in a viable state is provided. The dried composition consists essentially of dried viable aerobic bacteria and an appropriate growth medium. The bacteria and growth medium are initially placed in an aqueous solution of 10 mM to 200 mM trehalose and a divalent cation, and dried at room temperature.
    Type: Grant
    Filed: September 24, 1998
    Date of Patent: August 26, 2003
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Alfred J. Mateczun, Leonard F. Peruski, Jr.
  • Publication number: 20030027241
    Abstract: Disclosed are methods and devices for detection of bacteria based on recognition and infection of one or more selected strains of bacteria with bacteriophage genetically modified to cause production of an inducer molecule in the bacterium following phage infection. The inducer molecule is released from the infected bacterium and is detected by genetically modified bacterial bioreporter cells designed to emit bioluminescence upon stimulation by the inducer. Autoamplification of the bioluminescent signal permits detection of low levels of bacteria without sample enrichment. Also disclosed are methods of detection for select bacteria, and kits for detection of select bacteria based on the described technology.
    Type: Application
    Filed: July 20, 2001
    Publication date: February 6, 2003
    Inventors: Gary S. Sayler, Steven A. Ripp, Bruce M. Applegate
  • Patent number: 6447784
    Abstract: The present invention is directed to mutant Salmonella sp. having a genetically modified msbB gene in which the mutant Salmonella is capable of targeting solid tumors. The invention is also directed to Salmonella sp. containing a genetically modified msbB gene as well as an genetic modification in a biosynthetic pathway gene such as the purI gene. The present invention further relates to the therapeutic use of the mutant Salmonella for growth inhibition and/or reduction in volume of solid tumors.
    Type: Grant
    Filed: September 8, 1998
    Date of Patent: September 10, 2002
    Assignees: Vion Pharmaceuticals, Inc., Yale University
    Inventors: David Bermudes, Kenneth Brooks Low
  • Patent number: 6376228
    Abstract: A method for vaccinating poultry to prevent salmonellosis and other microbial-related health problems in humans is described. The method involves isolation of a poultry heterophil-adapted strain of a microorganism that may be used in a vaccine. A vaccine comprising a preparation of the poultry heterophil-adapted strain is administered to poultry to reduce the transmission of microorganisms causing salmonellosis and other illnesses.
    Type: Grant
    Filed: May 23, 2000
    Date of Patent: April 23, 2002
    Assignee: Iowa State University Research Foundation, Inc.
    Inventor: Theodore T. Kramer
  • Patent number: 6316244
    Abstract: A bacterial autoinducer and method for isolating and purifying a bacterial autoinducer from a sample comprising the steps of collecting a sample containing the autoinducer, fractionating the sample to isolate fractions corresponding to molecular weights of approximately 300-1500 Dalton, and eluting the isolate on an anion-exchange chromatographic column and selecting the faction containing the autoinducer.
    Type: Grant
    Filed: February 28, 2000
    Date of Patent: November 13, 2001
    Assignee: University of Leicester
    Inventors: Primrose Pamela Elaine Freestone, Peter Humphrey Williams, Mark Lyte, Richard David Haigh
  • Patent number: 6190669
    Abstract: Attenuated Salmonella mutants which constitutively express the Vi antigen are disclosed, as well as vaccines against typhoid fever containing the same, live vector vaccines containing the same, and DNA-mediated vaccines containing the same.
    Type: Grant
    Filed: May 13, 1998
    Date of Patent: February 20, 2001
    Assignee: University of Maryland, Baltimore
    Inventors: Fernando R. Noriega, Marcelo B. Sztein, Myron M. Levine
  • Patent number: 6120774
    Abstract: A method for vaccinating poultry to prevent salmonellosis and other microbial-related health problems in humans is described. The method involves isolation of a poultry heterophil-adapted strain of a microorganism that may be used in a vaccine. A vaccine comprising a preparation of the poultry heterophil-adapted strain is administered to poultry to reduce the transmission of microorganisms causing salmonellosis and other illnesses.
    Type: Grant
    Filed: February 14, 1997
    Date of Patent: September 19, 2000
    Assignee: Iowa State University Research Foundation, Inc.
    Inventor: Theodore T. Kramer
  • Patent number: 5855879
    Abstract: This invention provides immunogenic compositions for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of S. typhi. The derivatives having a mutation of the cya and/or crp and/or cdt genes. The invention also provides immunogenic compositions for the immunization of a vertebrate and invertebrate comprising an avirulent derivative of the above type which is capable of expressing a recombinant gene derived from a pathogen of said vertebrate or invertebrate individual to produce an antigen capable of inducing an immune response against said pathogen. Other embodiments of the invention include methods of preparing immunogenic compositions from these strains, and strains useful in the preparation of the immunogenic compositions, as well as methods of stimulating the immune system to respond to an immunogenic antigen of S. typhi by administration of the immunogenic composition.
    Type: Grant
    Filed: March 10, 1994
    Date of Patent: January 5, 1999
    Assignee: Washington University
    Inventor: Roy Curtiss III
  • Patent number: 5855880
    Abstract: This invention provides immunogenic compositions for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of S. typhi. The derivatives having a mutation of the cya and/or crp and/or cdt genes. The invention also provides immunogenic compositions for the immunization of a vertebrate and invertebrate comprising an avirulent derivative of the above type which is capable of expressing a recombinant gene derived from a pathogen of said vertebrate or invertebrate individual to produce an antigen capable of inducing an immune response against said pathogen. Other embodiments of the invention include methods of preparing immunogenic compositions from these strains, and strains useful in the preparation of the immunogenic compositions, as well as methods of stimulating the immune system to respond to an immunogenic antigen of S. typhi by administration of the immunogenic composition.
    Type: Grant
    Filed: February 5, 1996
    Date of Patent: January 5, 1999
    Assignee: Washington University
    Inventors: Roy Curtiss, III, Sandra M. Kelly
  • Patent number: 5792622
    Abstract: A microbiological assay for chemicals, which uses a cell and a reducing dye to quantitatively measure inhibition of electron transport in the cell membrane as a function of chemicals in the substance being tested, is disclosed. This assay and method is reliable, simple, fast, and inexpensive, requires a minimum amount of durable equipment, and avoids the need for the use of live animals as the indicator organisms. The assay is particularly useful for testing for toxicity in food products, environmental, medical and industrial processes, sewage treatment, effluent, agricultural wastes, and chemical dumps.
    Type: Grant
    Filed: November 16, 1995
    Date of Patent: August 11, 1998
    Assignee: New Mexico State University Technology Transfer Corporation
    Inventor: James L. Botsford
  • Patent number: 5786167
    Abstract: The invention comprises a culture medium and method for distinguishing bacteria of Salmonella species from other gram-negative bacteria, especially those belonging to the family Enterobacteriaceae. It is based on the ability of salmonellae to utilize melibiose, mannitol, and sorbitol and convert them into acids, together with a chromogenic substrate used for identifying .beta.-galactosidase. Other bacteria of the family Enterobacteriaceae, most of which are .beta.-galactosidase-positive, appear as brown, blue, or green colonies, depending on the chromogenic substrate used. Apart from Salmonella species, other .beta.-galactosidase-negative bacteria, such as Proteus species, appear as colorless colonies. Salmonellae can be identified directly on the culture medium after incubation, by their characteristic bright red color.
    Type: Grant
    Filed: November 22, 1996
    Date of Patent: July 28, 1998
    Assignee: Orion-Yhtymae Oy
    Inventors: Helena Tuompo, Leena Scheinin, Marita Jussila, Irmeli Laine
  • Patent number: 5750363
    Abstract: A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to an antimicrobial agent. The method includes providing at least one receptacle containing an aqueous solution that does not contain a carbon source and inoculating the solution with the specimen. The method further includes placing into the receptacle (i) a slide having bound thereto a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.
    Type: Grant
    Filed: May 19, 1997
    Date of Patent: May 12, 1998
    Assignee: Infectech, Inc.
    Inventors: Robert-A. Ollar, Mitchell S. Felder
  • Patent number: 5726031
    Abstract: A test method and medium for quantitatively identifying and distinguishing biological materials in a test sample. A first biological material has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has specificity for one of the substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes. The first and second biological materials are capable of fermenting a sugar, and the third material does not ferment sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon acidification due to fermentation, resulting in the formation of a zone of a third color around the water insoluble compounds of the sugar-fermenting materials.
    Type: Grant
    Filed: March 26, 1996
    Date of Patent: March 10, 1998
    Assignee: RCR Scientific, Inc.
    Inventors: Jonathan N. Roth, Gordon L. Bontrager
  • Patent number: 5622825
    Abstract: A novel method for conjugating haptens, ligands or luminescent labels to polynucleotides has been developed. This method involves the formation of a mixed anhydride, followed by reaction of the mixed anhydride intermediate with a nucleophilic group-containing polynucleotide in a DMF/water solvent system. The resulting compound finds usefulness especially in the gene probe area.
    Type: Grant
    Filed: December 19, 1994
    Date of Patent: April 22, 1997
    Assignee: Ciba Corning Diagnostics Corp.
    Inventors: Say-Jong Law, Hana Lukinsky
  • Patent number: 5580557
    Abstract: Disclosed herein are methods for attenuating virulent gram negative bacteria to produce avirulent bacteria. The methods comprise passaging the wild-type bacteria through phagocytic cells, such as macrophages or polymorphonuclear leukocytes, or through lysosomes derived from such cells, a sufficient number of times until the bacteria become avirulent to the animal host. The bacteria are preferably from the family Enterobacteracea and most preferably from the genus Salmonellae. The invention further comprises the avirulent bacteria produced by the methods, pure cultures of such bacteria, and methods of using the bacteria, preferably in a vaccine for administration to an animal host to induce an immune response to the wild-type gram negative bacteria in the host.
    Type: Grant
    Filed: May 24, 1995
    Date of Patent: December 3, 1996
    Assignee: Iowa State University Research Foundation, Inc.
    Inventor: Theodore T. Kramer
  • Patent number: 5529910
    Abstract: A method for testing causative bacterial species of food poisoning which is characterized by using two oligonucleotide primers that hybridize to opposite strands of bacterial DNA specifically, and flank a unique region in the target DNA and amplifying the specific fragment of the bacterial DNA, comprising the steps of:(a) hybridizing the primer to specific gene sequence of bacteria in a sample, extending the hybridized primer with deoxynucleotide triphosphates (dATP, dCTP, dGTP, and dTTP), and resultantly making the double strand nucleotide;(b) where the primer extension products are cleaved into each single strand of nucleotide by certain external force such as heat, pH and so on, one single strand functioning as a template for nucleotide extension with a primer of the other strand;(c) repeating a series of cycles involving cleavage of primer extension products, primer hybridizing, extension of the hybridized primers to amplify the specific fragment of DNA, and detecting the amplified DNA fragment; and(d) as
    Type: Grant
    Filed: September 27, 1993
    Date of Patent: June 25, 1996
    Assignee: Shimadzu Corporation
    Inventors: Tetsuo Ohashi, Hiroyuki Jikuya, Jun Takano, Yoshinari Shirasaki, Hirohisa Abe, Koichi Yamagata, Yoshihiro Aoyama, Jun Tada, Shigeru Fukushima
  • Patent number: 5436001
    Abstract: Disclosed herein are methods for attenuating virulent gram negative bacteria to produce avirulent bacteria. The methods comprise passaging the wild-type bacteria through phagocytic cells, such as macrophages or polymorphonuclear leukocytes, or through lysosomes derived from such cells, a sufficient number of times until the bacteria become avirulent to the animal host. The bacteria are preferably from the family Enterobacteracea and most preferably from the genus Salmonellae. The invention further comprises the avirulent bacteria produced by the methods, pure cultures of such bacteria, and methods of using the bacteria, preferably in a vaccine for administration to an animal host to induce an immune response to the wild-type gram negative bacteria in the host.
    Type: Grant
    Filed: July 19, 1993
    Date of Patent: July 25, 1995
    Assignee: Iowa State University Research Foundation
    Inventor: Theodore T. Kramer
  • Patent number: 5405754
    Abstract: The invention described herein consists of a process for preparing an antigenic reagent useful for the indirect determination of Salmonella typhi, the organism that is the causal agent of typhoid fever (TF). The invention consists on the following steps: to grow Salmonella typhi in a culture medium, characterized by containing a free-iron chelator, which generates a specific S. typhi outer membrane protein (OMP) pattern, OMPs that are used as a selective antigen for the detection of specific serum antibodies, by an immunoassay technique (ELISA).
    Type: Grant
    Filed: March 21, 1991
    Date of Patent: April 11, 1995
    Assignee: Universidad Nacional Autonoma de Mexico
    Inventors: Edmundo Calva-Mercado, Guillermo M. Ruiz Palacios, Antonio V. Rodriguez, Yolanda L. Vidal
  • Patent number: 5387744
    Abstract: This invention provides immunogenic compositions for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of S. typhi. The derivatives having a mutation of the cya and/or crp and/or cdt genes. The invention also provides immunogenic compositions for the immunization of a vertebrate and invertebrate comprising an avirulent derivative of the above type which is capable of expressing a recombinant gene derived from a pathogen of said vertebrate or invertebrate individual to produce an antigen capable of inducing an immune response against said pathogen. Other embodiments of the invention include methods of preparing immunogenic compositions from these strains, and strains useful in the preparation of the immunogenic compositions, as well as methods of stimulating the immune system to respond to an immunogenic antigen of S. typhi by administration of the immunogenic composition.
    Type: Grant
    Filed: July 7, 1993
    Date of Patent: February 7, 1995
    Assignee: Washington University
    Inventors: Roy Curtiss, III, Sandra M. Kelly
  • Patent number: 5374423
    Abstract: Cytokine receptors for tumor necrosis factor e which are found on microorganisms may, if bound with exogenous TNF.alpha., enhance the response of natural killer cells activated by the microorganisms, or increase TNF.alpha. production by peripheral blood lymphocytes treated with the microorganisms. Microorganisms with receptor-bound exogenous TNF.alpha. have enhanced cellular invasion ability which may change the immune response thereto. Clinical and pharmaceutical applications of these discoveries are provided.
    Type: Grant
    Filed: August 27, 1993
    Date of Patent: December 20, 1994
    Assignee: Board of Regents, The University of Texas System
    Inventors: Gary R. Klimpel, David W. Niesel
  • Patent number: 5356778
    Abstract: The invention relates to a method of detecting gram-negative bacterial endotoxin using antibody capture combined with amoebocyte lysate chromogenic detection. The method is highly sensitive and rapid and may be used for detection of specific endotoxin. In a particular application, picogram levels of Haemophilus influenzae type b endotoxin are detected in plasma taken from previously infected mammals. In another particular application, the method is applied to the detection and diagnosis of disease, through the detection of endotoxin from disease-causing organisms. A specific example is the diagnosis of chancroid through the detection of endotoxin from H. ducreyi.
    Type: Grant
    Filed: February 5, 1993
    Date of Patent: October 18, 1994
    Assignee: Board of Regents, The University of Texas
    Inventors: Eric J. Hansen, Robert S. Munford, Jussi Mertsola
  • Patent number: 5294441
    Abstract: This invention provides immunogenic compositions for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of S. typhi. The derivatives having a mutation of the cya and/or crp and/or cdt genes. The invention also provides immunogenic compositions for the immunization of a vertebrate and invertebrate comprising an avirulent derivative of the above type which is capable of expressing a recombinant gene derived from a pathogen of said vertebrate or invertebrate individual to produce an antigen capable of inducing an immune response against said pathogen. Other embodiments of the invention include methods of preparing immunogenic compositions from these strains, and strains useful in the preparation of the immunogenic compositions, as well as methods of stimulating the immune system to respond to an immunogenic antigen of S. typhi by administration of the immunogenic composition.
    Type: Grant
    Filed: November 7, 1991
    Date of Patent: March 15, 1994
    Assignee: Washington University
    Inventor: Roy Curtiss, III
  • Patent number: 5270038
    Abstract: Cytokine receptors for tumor necrosis factor .alpha. which are found on microorganisms may, if bound with exogenous TNF.alpha., enhance the response of natural killer cells activated by the microorganisms, or increase TNF.alpha. production by peripheral blood lymphocytes treated with the microorganisms. Microorganisms with receptor-bound exogenous TNF.alpha. have enhanced cellular invasion ability which may change the immune response thereto. Clinical and pharmaceutical applications of these discoveries including vaccines with increased efficacy are provided.
    Type: Grant
    Filed: January 23, 1992
    Date of Patent: December 14, 1993
    Assignee: Board of Regents, The University of Texas System
    Inventors: Gary R. Klimpel, David W. Niesel
  • Patent number: 5215919
    Abstract: A (1R,2S)-2-hydroxycycloalkanecarboxylic acid ester is efficiently and selectively produced by microbial asymmetric reduction of a 2-oxocycloalkanecarboxylic acid ester with a bacterial strain or its processed material.
    Type: Grant
    Filed: February 19, 1992
    Date of Patent: June 1, 1993
    Assignee: Takeda Chemical Industries, Ltd.
    Inventors: Hiroyuki Miya, Mitsuru Kawada, Yoshio Sugiyama
  • Patent number: 5210035
    Abstract: Live vaccines are provided and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification in a gene involved in at least one, normally at least two, biosynthetic pathways involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step, e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants require aromatic amino acids, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid or a highly concentrated source of absorabable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence while retaining the desired immunogenicity to initiate the immunogenic response. Various techniques can be employed for providing the desired change.
    Type: Grant
    Filed: August 16, 1991
    Date of Patent: May 11, 1993
    Assignee: Board of Trustees of Leland Stanford Jr. University
    Inventor: Bruce A. D. Stocker
  • Patent number: 5198339
    Abstract: The invention relates to a method of detecting gram-negative bacterial endotoxin using antibody capture combined with amoebocyte lysate chromogenic detection. The method is highly sensitive and rapid and may be used for detection of specific endotoxin. In a particular application, picogram levels of Haemophilus influenzae are detected in plasma taken from previously infected mammals.
    Type: Grant
    Filed: July 13, 1990
    Date of Patent: March 30, 1993
    Assignee: Board of Regents, The University of Texas System
    Inventors: Eric J. Hansen, Robert S. Munford, Jussi Mertsola
  • Patent number: 5194374
    Abstract: The present invention relates to an isolating medium for the identification of the Salmonella bacterium, wherein a polyol metabolizable by Salmonella and a pH indicator reacting to acidification are added to a culture support containing peptones.
    Type: Grant
    Filed: January 10, 1992
    Date of Patent: March 16, 1993
    Assignee: EUREC
    Inventor: Alain Rambach
  • Patent number: 5162226
    Abstract: Non-virulent aro-Salmonella typhimurium bacterium is disclosed into which has been cloned a heterologous nucleotide sequence encoding for the expression of Streptococcal M protein antigen from S. pyogenes seratype 5, which is effective to elicit opsonic antibodies against Streptococcal infections. The bacterium is useful for vaccination against Streptococcus pyogenes bacteria.
    Type: Grant
    Filed: August 24, 1987
    Date of Patent: November 10, 1992
    Assignee: University of Tennessee Research Corp. (U.T.R.C.)
    Inventors: Edwin H. Beachey, Thomas P. Poirier, Michael A. Kehoe
  • Patent number: 5147778
    Abstract: Improved nucleic acid probes capable of specifically hybridizing to rRNA of Salmonella and not to rRNA of non-Salmonella are described along with methods utilizing such probes for the detection of Salmonella in food and other samples.
    Type: Grant
    Filed: November 29, 1988
    Date of Patent: September 15, 1992
    Assignee: Amoco Corporation
    Inventors: Raymond M. Nietupski, Stephen G. Wilson, Jyotsna Shah, Samuel W. Chan, Donald N. Halbert, David J. Lane
  • Patent number: 5112749
    Abstract: The present invention is directed to attenuated strains of enteroinvasive bacteria that express a peptide or protein related to an epitope of the malaria parasites of the genus Plasmodium. The bacterial strains of the invention which can multiply in a host without causing significant disease or disorder, and which express a Plasmodium-related peptide that induces a protective immune response against malaria, can be used in live vaccine formulations for malaria. In specific embodiments, a Plasmodium-related peptide can be expressed as a fusion protein, for example, with a bacterial enterotoxin.The invention also relates to methods for expression of malaria antigens or fragments thereof within attenuated enteroinvasive bacteria.In particular embodiments, the invention is directed to the expression by attenuated Salmonella spp. of epitopes of Plasmodium circumsporozoite proteins.
    Type: Grant
    Filed: October 2, 1987
    Date of Patent: May 12, 1992
    Assignee: Praxis Biologics, Inc.
    Inventors: Robert N. Brey, III, William R. Majarian, Subramonia Pillai, Wayne T. Hockmeyer
  • Patent number: 5084565
    Abstract: Nucleic acid probes capable of specifically hybridizing to rRNA of E. coli and Shigella species and not to rRNA of non-E. coli/Shigella are described along with methods utilizing such probes for the specific detection of E. coli and/or Shigella in food and other samples.
    Type: Grant
    Filed: August 18, 1988
    Date of Patent: January 28, 1992
    Assignee: Gene-Trak Systems
    Inventors: Kyriaki Parodos, Hsien-Yeh Hsu, David Sobell, Janice M. McCarty, David J. Lane
  • Patent number: 5079165
    Abstract: Methods and compositions are provided for the cloning and expression of plasmids bearing genes coding for the non-toxic subunit of the heat-laible enterotoxin (LT-B) of E. coli. These plasmids may be cloned into stable avirulent strains of Salmonella typhi and used to make oral bivalent vaccines. Such vaccines may be used to prevent typhoid fever and cholera-like enterotoxin-induced diarrheal disease.
    Type: Grant
    Filed: June 29, 1988
    Date of Patent: January 7, 1992
    Assignee: Praxis Biologics, Inc.
    Inventors: John D. Clements, Sawsan E. El-Morshidy
  • Patent number: 5055394
    Abstract: This invention relates to a nucleic acid probe and method for the rapid detection of typhoid fever bacteria by use of a nucleic acid hybridization probe, equivalent to the DNA region encoding the Vi antigen of enteric bacteria such as Salmonella typhi, S. paratyphi C, or Citrobacter freundii, in a nucleic acid hybridization reaction with a clinical specimen containing typhoid fever bacteria.
    Type: Grant
    Filed: June 23, 1986
    Date of Patent: October 8, 1991
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventors: Dennis J. Kopecko, Louis S. Barson, Fran A. Rubin
  • Patent number: 5043264
    Abstract: A novel probe for detecting Salmonella comprises:a labeled substance, anda DNA or RNA fragment which hybridizes with a base sequence having the following formula (I), or (II) being complementary to the formula (I).5'-GCTCAGACGTATGGCGGTA-3' (I)3'-CGAGTCTGCATACCGCCAT-5' (II)This invention also provides a method for detecting Salmonella by using the probe.The probe reduces the time required for detecting Salmonella.
    Type: Grant
    Filed: July 6, 1989
    Date of Patent: August 27, 1991
    Assignee: Shimadzu Corporation
    Inventors: Hiroyuki Jikuya, Tetuo Ohashi, June Takano
  • Patent number: 5021234
    Abstract: An agent for the diagnosis and treatment of malignant tumors and for the treatment of lowered cellular and humoral immune defense is described which contains an immune modulator, a lipopolysaccharide or an immune modulator tagged with a radioactive tracer, a dyestuff or a cytostatic, or a similar polysaccharide, possibly in and/or on liposomes or lipidized. There is also described a product which contains the agent together with an adjuvant consisting of an aldehyde and an alcohol. There is also described a method for the diagnosis of malignant tumors comprising the step of administering to a mammal a diagnostic effective amount of the agent, optionally together with an adjuvant consisting of an aldehyde and an alcohol.
    Type: Grant
    Filed: February 26, 1990
    Date of Patent: June 4, 1991
    Inventor: Udo Ehrenfeld
  • Patent number: 4943521
    Abstract: A method for rapidly and reliably determining the potential carcinogenic activity of hydrocarbon mixtures which is especially useful for those of petroleum origin. A sample of the mixture is nitrated under conditions effective to convert the polynuclear aromatic hydrocarbons to their nitrated derivatives, and without separation of the nitrated components, the product is incubated with an inoculum of Salmonella typhimurium tester strain T98. The excessive production of revertant colonies is a measure of the mutagenic activity of the oil, and this measure is shown to correlate with dermal carcinogenic activity.
    Type: Grant
    Filed: February 24, 1987
    Date of Patent: July 24, 1990
    Assignee: Mobil Oil Corporation
    Inventors: Gary R. Blackburn, Carl R. Mackerer, Ceinwen A. Schreiner
  • Patent number: 4920063
    Abstract: Methods and associated devices for detecting the presence of a particular motile organism within a sample are disclosed. The sample may be derived from dry milk, raw meat, poultry or a clinical specimen. A preferred method includes inoculating a selective enrichment medium containing a chemotactic attractant with the sample and contacting the selective enrichment medium with a nonselective motility medium containing a chemotatic attractant in a concentration less than the attractant concentration in the selective enrichment medium. Upon incubation, the motile organism metabolizes the chemotactic attractant, allowing the organism to move into the motility medium where it interacts with antibodies specific for the organism, thereby causing the formation of a persistent immobilization band. The methods are particularly useful in detecting Salmonella.
    Type: Grant
    Filed: September 9, 1985
    Date of Patent: April 24, 1990
    Assignee: BioControl Systems, Inc.
    Inventor: N. Robert Ward, Jr.
  • Patent number: 4900348
    Abstract: Compost, e.g. hardwood bark, is rendered suppressive to plant pathogens, such as Rhizoctonia solani, Pythium ultimum and Fusarium, and/or diseases caused thereby by adding to the compost, desirably after peak heating has been achieved but before substantial recolonization of the compost by mesophilic microorganisms has occurred, one or more microorganisms antagonistic to the plant pathogen. Container media also is rendered suppressive to plant pathogens and/or diseases caused thereby by amending the media with the just-described prepared suppressive compost or, alternatively, by amending separately with the compost and with Trichoderma fungus and antagonistic bacterium separately or mixed together. Desirably, the inoculated antagonistic microorganisms comprise Trichoderma hamatum species A.T.C.C. No. 20765 or 20764, together with Xanthomonas maltophilia bacterium species A.T.C.C. No. 53199 or a Flavobacterium balustinum isolate 299, A.T.C.C. No. 53198 species, A.T.C.C. No. 53198.
    Type: Grant
    Filed: February 6, 1987
    Date of Patent: February 13, 1990
    Assignee: The Ohio State University Research Foundation
    Inventor: Harry A. Hoitink
  • Patent number: 4837151
    Abstract: Live vaccines are provided and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification in a gene involved in at least one, normally at least two, biosynthetic pathways, involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants require aromatic amino acids, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence, while retaining the desired immunogenicity to initiate the immunogenic response. Various techniques can be employed for providing the desired change.Salmonella typhimurium strain SL1479 was deposited at the ATCC on Sept.
    Type: Grant
    Filed: November 14, 1985
    Date of Patent: June 6, 1989
    Assignee: The Board of Trustees of the Leland Stanford Junior University, Stanford University
    Inventor: Bruce A. D. Stocker
  • Patent number: 4788057
    Abstract: A method of treating a human patient to effect the remission of symptoms associated with psoriasis, which comprises parenterally administering, in multiple injections, to the patient typhoid vaccine in a therapeutically effective amount which is sufficient to provide immunostimulating activity.
    Type: Grant
    Filed: October 2, 1987
    Date of Patent: November 29, 1988
    Inventor: Salvatore J. Catapano
  • Patent number: 4783403
    Abstract: L-phenylalanine is produced by using a microorganism belonging to the species Citrobacter freundii, Erwinia herbicola, Enterobacter cloacae, Klebsiella oxytoca, Salmonella typhimurium, Bacillus cereus, Flavobacterium suaveolens, Serratia marcescens, Pseudomonas putida, Enterobacter cloacae, Proteus mirabilis, Paracoccus denitrificans, Arthrobacter globiformis, Bacillus sphaericus, Corynebacterium hydrocarboclastus, Kluyvera micum or Microbacterium ammoniaphilum and having the ability to convert phenylpyruvic acid into L-phenylalanine in the presence of an amino group donor; or fumaric acid and ammonium ion or urea.
    Type: Grant
    Filed: February 1, 1985
    Date of Patent: November 8, 1988
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kazumi Araki, Toshitsugu Ozeki, Yukiyoshi Ito, Shuichi Ishino, Hideharu Anazawa, Shigeru Kamimori
  • Patent number: 4764370
    Abstract: A vaccine against a microbial pathogen comprised of a live, immunogenic but prototrophic and avirulent mutant strain of the selected microbial pathogen in an amount effective to confer immunity. A method of obtaining a vaccine that induces a heightened cellular and humoral immune response to one of a variety of microbial pathogens in a warm blooded animal. A method for isolation of an avirulent strain of a selected pathogenic microorganism.
    Type: Grant
    Filed: September 12, 1984
    Date of Patent: August 16, 1988
    Assignee: Scripps Clinic and Research Foundation
    Inventors: Patricia I. Fields, Constantine G. Haidaris, Frederick L. Heffron