Particle Count Or Volume Standard Or Control (e.g., Platelet Count Standards, Etc.) Patents (Class 436/10)
  • Patent number: 10345303
    Abstract: Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: July 9, 2019
    Assignee: Theranos IP Company, LLC
    Inventors: Elizabeth A. Holmes, Chinmay Pangarkar, Timothy Smith, Karan Mohan, Samartha Anekal, Daniel Young, James Wasson
  • Patent number: 9915598
    Abstract: The present disclosure relates to compositions comprising a hydrogel particle with optical properties substantially similar to the optical properties of a target cell, and methods for their use.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: March 13, 2018
    Assignee: SLINGSHOT BIOSCIENCES
    Inventors: Jeffrey Kim, Oliver Liu, Jeremy Agresti, Anh Tuan Nguyen
  • Patent number: 9891210
    Abstract: This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: February 13, 2018
    Assignee: SYSMEX CORPORATION
    Inventors: Hideaki Matsumoto, Kinya Uchihashi, Yuji Itose, Aya Konishi
  • Patent number: 9765128
    Abstract: The present invention provides isolated stabilized EPO-derived peptides and their mimics that protect against tissue damage in subjects having diverse forms of neural and non-neural organ system injury, pharmaceutical compositions containing the isolated stabilized EPO-derived peptides, methods for treating symptoms of a disease, disorder or condition having an inflammatory or an autoimmune component in a subject in need thereof, and methods for downregulating immune mediator activity in a subject in need thereof.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: September 19, 2017
    Inventor: Peter C. Dowling
  • Patent number: 9757729
    Abstract: Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: September 12, 2017
    Assignee: California Institute of Technology
    Inventors: Yu-Chong Tai, Siyang Zheng, Jeffrey Chun-Hui Lin, Harvey L. Kasdan
  • Patent number: 9714897
    Abstract: Hydrogel particles and their use in cytometic applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell. In this regard, the hydrogel particles provided herein in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.
    Type: Grant
    Filed: February 8, 2016
    Date of Patent: July 25, 2017
    Assignee: Slingshot Biosciences, Inc.
    Inventors: Jeffrey Kim, Oliver Liu, Jeremy Agresti, Anh Tuan Nguyen
  • Patent number: 9551600
    Abstract: A system for creating a flow cytometer network includes: a flow cytometer with an interrogation zone and a fixed gain detection system that collects sample data from the interrogation zone; a flow cytometer data center that stores and manages sample-related data from the flow cytometer; and a network communication module that communicates sample-related data between the flow cytometer and the data center. The system may include a second flow cytometer and a second network communication module, where the first and second flow cytometers are calibrated to have substantially identical fixed gain settings. A method for creating a flow cytometer network includes: calibrating first and second flow cytometers with a calibration solution; collecting sample data with a fixed gain detection system; uploading data to a flow cytometer data center; retrieving data from the data center; and performing data analysis on the retrieved data.
    Type: Grant
    Filed: June 14, 2011
    Date of Patent: January 24, 2017
    Assignee: Accuri Cytometers, Inc.
    Inventors: Grant C. Howes, Collin A. Rich
  • Patent number: 9513227
    Abstract: The present invention provides a method for quantitative determination of oxidant which method is capable of accurately and rapidly performing quantitative determination of oxidant at low cost, and an apparatus for quantitative determination of oxidant used in the method. The method for quantitative determination of oxidant according to the present invention is a method for quantitative determination of oxidant performing quantitative determination of oxidant in a sample using a redox reaction, the method including: adding one kind of reducing agent to a sample solution containing one or a plurality of kinds of oxidants having different lifetimes; producing an absorbance curve by measuring a time change in absorbance of the post-color-change or post-coloring reducing agent; and performing the quantitative determination of the oxidant while identifying the oxidant in the sample solution based on the obtained absorbance curve.
    Type: Grant
    Filed: April 21, 2014
    Date of Patent: December 6, 2016
    Assignee: Panasonic Intellectual Property Management Co., Ltd.
    Inventors: Mari Onodera, Shin-ichi Imai, Hironori Kumagai
  • Patent number: 9429501
    Abstract: Provided is a treatment method for damaging an erythrocyte and a leukocyte while suppressing damage to cells other than blood cells present in blood. In an embodiment, the disclosure relates to a method for treating a sample containing blood components, the method including mixing a sample containing blood components with a surfactant A, where the surfactant A is a nonionic surfactant represented by General formula R1—O-(EO)n-R2 (I).
    Type: Grant
    Filed: June 18, 2013
    Date of Patent: August 30, 2016
    Assignee: ARKRAY, Inc.
    Inventor: Hidenori Takagi
  • Patent number: 9267956
    Abstract: Methods for producing stimulated, positive and negative control reference standard for monitoring intracellular cytokine levels and cytokine release in test samples by stimulating cells to produce cytokines in the presence of a cytokine release inhibitor, fixing the stimulated cells with a fixative such as paraformaldehyde, washing to remove excess fixatives and freeze-drying the stimulated, fixed cells. Methods for producing labeled reference standards for cell proliferation assays are also disclosed, in which proliferation-competent mammalian cells, isolated from a human or animal body are labeled with a label, such as a dye, that is divided between daughter cells during cell proliferation (e.g., carboxyfluorescein succinimidyl ester), the cells are stimulated to proliferate, the proliferated cells are fixed by addition of a fixative and then preserved by freeze drying or cryopreservation.
    Type: Grant
    Filed: July 9, 2009
    Date of Patent: February 23, 2016
    Assignee: SECRETARY OF STATE FOR HEALTH
    Inventor: Richard John Stebbings
  • Patent number: 9217695
    Abstract: Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.
    Type: Grant
    Filed: May 21, 2010
    Date of Patent: December 22, 2015
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: James Winkelman, Milenko Tanasijevic, David Zahniser
  • Patent number: 9143581
    Abstract: Systems and methods are used to generate a protocol for an assay. At least one performance characteristic parameter of an assay and at least one standardized protocol for each assay of a plurality of assays and assay types are stored. A performance characteristic parameter selection and an assay selection are received from a client device of a laboratory. One or more performance characteristic parameters and a standardized protocol are retrieved from the database device. The client device is sent the one or more performance characteristic parameters and one or more study variable values. One or more amendments to the one or more performance characteristic parameters and one or more study variable values are received from the client device. A protocol for the assay is generated based on the one or more amendments.
    Type: Grant
    Filed: November 5, 2013
    Date of Patent: September 22, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jerry Boonyaratanakornkit, Erhard Schoenbrunner, Marc Balcer
  • Patent number: 9116093
    Abstract: A blood analyzer, a blood analysis method, and a computer program product that can distinguishably detect abnormal lymphocytes, blasts, and atypical lymphocytes are provided. A blood analyzer prepares a first measurement sample from a first reagent containing a hemolyzing agent, a second reagent containing a fluorescence staining dye, and the blood specimen, and prepares a second measurement sample from a third reagent containing a hemolyzing agent, a fourth reagent containing a fluorescence staining dye, and the blood specimen. The blood analyzer measures each of the measurement samples, and distinguishably detects abnormal lymphocytes, blasts, and atypical lymphocytes in a blood specimen based on the measurement data.
    Type: Grant
    Filed: May 2, 2012
    Date of Patent: August 25, 2015
    Assignee: SYSMEX CORPORATION
    Inventors: Masaki Abe, Ayumu Yoshida, Jo Linssen
  • Patent number: 9103759
    Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
    Type: Grant
    Filed: April 26, 2012
    Date of Patent: August 11, 2015
    Assignee: Abbott Laboratories
    Inventors: Jiong Wu, Marilou Coleman, Michael R. Buhl, Giacomo Vacca, Emily H. Lin
  • Patent number: 9092034
    Abstract: The present invention relates to a vacuum-driven fluidic system for a flow-type particle analyzer. The system includes a vacuum pump which creates a pressure drop downstream of the flow cell, which pulls sheath fluid and sample fluid through the flow cell. A variable-resistance fluidic resistor is configured to control the ratio of sample fluid flow to sheath fluid flow. Dual feedback circuits, one configured to modulate the vacuum pump power in response to the pressure drop across the flow cell, referred to as the dynamic pressure drop, and a second configured to modulate the vacuum pump power in response to the pressure drop created by the vacuum pump relative to ambient pressure, referred to as the static pressure drop, are used to automatically control the system. The present invention enables adjustment of the sample fluid flow rate while maintaining a constant total fluid flow through the flow cell, and further, enables pausing the system without significant fluctuations in the vacuum.
    Type: Grant
    Filed: August 6, 2013
    Date of Patent: July 28, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: David Vrane, Pierce O. Norton
  • Patent number: 9081021
    Abstract: A reagent for measuring platelets comprising Nile Blue hydrogensulfate, or Nile Blue and an acid, a reagent kit for measuring platelets comprising the reagent for measuring platelets, and a method for measuring platelets using the reagent or reagent kit.
    Type: Grant
    Filed: February 17, 2009
    Date of Patent: July 14, 2015
    Assignee: SYSMEX CORPORATION
    Inventors: Yuhgi Suzuki, Keiko Moriyama, Yusuke Mori, Hiroki Takeshita
  • Patent number: 9068915
    Abstract: A method and system for calibrating a flow cytometer to a refractive index X, the method comprising: analyzing a first mixture of particles in the cytometer and recording the results produced, wherein the first mixture comprises particles having refractive index X, wherein the particles of refractive index X have a plurality of different sizes; analyzing a second mixture of particles in the cytometer and recording the results produced, wherein the second mixture comprises particles having refractive index Y, wherein refractive index Y does not equal refractive index X, and wherein the particles of refractive index Y have a plurality of different sizes, wherein there is at least a partial overlap between the particle sizes in the first and second mixtures, and wherein the size of at least one particle of the second mixture is known; and using the results produced by the first and second mixture to calibrate the cytometer to refractive index X.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: June 30, 2015
    Assignee: OJK Consulting Ltd.
    Inventor: Oliver Kenyon
  • Patent number: 9044430
    Abstract: Disclosed are products and methods for treating soft tissue injuries. The provided methods include the production of processed or cryopreserved microvascular tissue. Also provided are products and methods of using processed or cryopreserved microvascular tissue for the treatment of soft tissue injuries.
    Type: Grant
    Filed: March 17, 2012
    Date of Patent: June 2, 2015
    Assignee: MicroVascular Tissues, Inc.
    Inventors: Dale R. Peterson, Richard B. Emmitt
  • Patent number: 8962263
    Abstract: The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.
    Type: Grant
    Filed: April 26, 2011
    Date of Patent: February 24, 2015
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Omar D. Perez, Garry P. Nolan
  • Patent number: 8951474
    Abstract: The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.
    Type: Grant
    Filed: February 5, 2010
    Date of Patent: February 10, 2015
    Assignee: On-Chip Biotechnologies Co., Ltd.
    Inventor: Kazuo Takeda
  • Patent number: 8936753
    Abstract: A blood coagulation analyzer includes a detector and a controller. The detector includes a light source for emitting light to a prepared measurement sample and a light-receiving section for receiving light transmitted through the measurement sample. The controller is configured to performs operations comprising acquiring, based on the light detected by the detector, ratio information reflecting a ratio between a first value reflecting the intensity of the light transmitted through the measurement sample of a clotting reaction starting stage and a second value reflecting the intensity of the light transmitted through the measurement sample of a clotting reacting ending stage, and acquiring, based on the ratio information, a fibrinogen concentration in the blood sample.
    Type: Grant
    Filed: September 29, 2010
    Date of Patent: January 20, 2015
    Assignee: Sysmex Corporation
    Inventors: Norimasa Yamamoto, Hiroyuki Fujino, Takamichi Naito, Naohiko Matsuo, Susumu Hoshiko
  • Patent number: 8911669
    Abstract: A hematology analyzer is provided. In certain embodiments, the hematology analyzer comprises: a) a flow cell; b) a light source for directing light to the flow cell; c) a plurality of detectors for detecting a plurality of optical characteristics of a blood cell passing through the flow cell; and d) a data analysis workstation programmed to: i. enumerate test blood cells passing through the flow cell; and ii. flag a blood sample as containing lysis-resistant red blood cells or fragile white blood cells.
    Type: Grant
    Filed: August 24, 2009
    Date of Patent: December 16, 2014
    Assignee: Abbott Laboratories
    Inventors: Martin Krockenberger, Richard B. Bordenkircher, Diana G. Garrett, John G. Glazier, James C. Bearden, Bodo Roemer, Giacomo Vacca
  • Patent number: 8906309
    Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by use of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by use of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.
    Type: Grant
    Filed: April 26, 2010
    Date of Patent: December 9, 2014
    Assignee: Abbott Laboratories
    Inventors: Martin Krockenberger, Jiong Wu, Bodo Roemer, Giacomo Vacca
  • Patent number: 8906308
    Abstract: A method for determining the volume or hemoglobin content of an individual red blood cell in a sample containing a population of red blood cells. The method may be performed on a hematology analyzer. Also provided are a hematology analyzer for performing the method and a computer-readable medium containing programming for performing the method.
    Type: Grant
    Filed: January 15, 2010
    Date of Patent: December 9, 2014
    Assignee: Abbott Laboratories
    Inventors: Martin Krockenberger, Diana G. Garrett, Giacomo Vacca
  • Patent number: 8900872
    Abstract: A method is provided for adjusting either the signal measured or activity calculated by an instrument or a predefined calibration curve for a medical diagnostic test, performed on an instrument with pre-calibrated reagents. A calibration adjuster for a blood clotting diagnostic test is further provided.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: December 2, 2014
    Assignee: Diagnostica Stago
    Inventors: Frederic Esteve, Lydie Nicoud
  • Patent number: 8889369
    Abstract: A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: November 18, 2014
    Assignee: Litron Laboratories, Ltd.
    Inventor: Stephen D. Dertinger
  • Patent number: 8883491
    Abstract: The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.
    Type: Grant
    Filed: April 8, 2009
    Date of Patent: November 11, 2014
    Assignee: Nexcelom Bioscience LLC
    Inventors: Bo Lin, Peter Y. Li, Jean Qiu, Timothy Smith, Todd Sobolewski, Alnoor Pirani
  • Patent number: 8865476
    Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: October 21, 2014
    Assignee: Life Technologies Corporation
    Inventors: Michael D. Ward, Carleton C. Stewart, Gregory Kaduchak
  • Patent number: 8865420
    Abstract: The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations.
    Type: Grant
    Filed: May 22, 2008
    Date of Patent: October 21, 2014
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Omar D. Perez, Garry P. Nolan, Jonathan M. Irish
  • Patent number: 8865470
    Abstract: The present invention reagents and methods for setting up an instruments having a multiplicity of detector channels for analyzing a multiplicity of fluorescent dyes. The present invention is particularly applicable in the field of flow cytometry.
    Type: Grant
    Filed: March 6, 2013
    Date of Patent: October 21, 2014
    Assignee: Becton, Dickinson and Company
    Inventors: Ming Yan, Alan M. Stall, Joseph T. Trotter, Robert A. Hoffman
  • Patent number: 8828737
    Abstract: Methods for using focused light scattering techniques for the optical sensing of biological particles suspended in a liquid medium are disclosed. The optical sensing enables one to characterize particles size and/or distribution in a given sample. This, in turn, allows one to identify the biological particles, determine their relative particle density, detect particle shedding, and identify particle aggregation. The methods are also useful in screening and optimizing drug candidates, evaluating the efficacy and dosage levels of such drugs, and in personalized medicine applications.
    Type: Grant
    Filed: July 14, 2009
    Date of Patent: September 9, 2014
    Assignee: Invitrox, Inc.
    Inventor: Don Gabriel
  • Patent number: 8815527
    Abstract: The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.
    Type: Grant
    Filed: April 26, 2011
    Date of Patent: August 26, 2014
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Omar D. Perez, Garry P. Nolan
  • Patent number: 8808624
    Abstract: This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information.
    Type: Grant
    Filed: November 9, 2010
    Date of Patent: August 19, 2014
    Assignee: Sysmex Corporation
    Inventors: Hideaki Matsumoto, Kinya Uchihashi, Yuji Itose, Aya Konishi
  • Patent number: 8796040
    Abstract: A system of quantitatively determining a biomolecule, which has: allowing fluorescent silica particles capable of emitting fluorescence detectable by a flow cytometer to capture a target biomolecule fluorescent-labelled for quantitative determination; detecting the fluorescence emitted from the fluorescent silica particles themselves by using the flow cytometer; and measuring the intensity of the fluorescence of the labelled target biomolecule, thereby quantitatively determining the target biomolecule.
    Type: Grant
    Filed: August 22, 2008
    Date of Patent: August 5, 2014
    Assignees: The Furukawa Electric Co., Ltd., The University of Tokushima
    Inventors: Hideki Aizawa, Michio Ohkubo, Michihiro Nakamura, Hirokazu Miyoshi
  • Patent number: 8790929
    Abstract: The invention provides a reagent for diluting a blood sample, comprising water, polyoxyethylene alkyl ether having a hydroxyl value of 52 to 60, and an osmo-regulator for regulating the osmotic pressure of the reagent in the range of 150 to 400 mOsm/kg, as well as a method for measuring the mean corpuscular volume of a blood sample.
    Type: Grant
    Filed: July 6, 2009
    Date of Patent: July 29, 2014
    Assignee: Sysmex Corporation
    Inventors: Yusuke Mori, Kinya Uchihashi, Yasuhiro Sakai
  • Patent number: 8765392
    Abstract: The present invention relates to methods and kits for diagnosing, ascertaining the clinical course of myelodysplastic syndrome (MDS) and ascertaining response to a therapy regimen of myelodysplastic syndrome. Specifically the invention provides methods and kits useful in the diagnosis and determination of clinical parameters associated with MDS based on surface markers unique to MDS.
    Type: Grant
    Filed: January 11, 2013
    Date of Patent: July 1, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Jonni Moore, Sundhu Cherian, Adam Bagg
  • Patent number: 8753888
    Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: June 17, 2014
    Assignee: The General Hospital Corporation
    Inventors: Denise L. Faustman, Douglas E. Burger
  • Patent number: 8748159
    Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: June 10, 2014
    Assignee: GenPrime, Inc.
    Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
  • Patent number: 8722419
    Abstract: Mass cytometry method. In one aspect, the method includes providing a sample having at least one cell type and mixing the sample with material such as nanoparticles functionalized with affinity molecules for the at least one cell type. The sample is transported through a suspended microchannel resonator to record a mass histogram and a cell count for the at least one cell type is determined from the histogram.
    Type: Grant
    Filed: June 15, 2007
    Date of Patent: May 13, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Scott R. Manalis, Thomas P. Burg, Michel Godin, Kenneth Babcock
  • Patent number: 8717045
    Abstract: A microorganism number-measuring apparatus includes: measurement container including measurement liquid; rotary driver; bacteria-collection signal generator; measurement signal generator; output amplifier for amplifying outputs of signal generators and; I/V amplifier; impedance measuring unit for measuring impedance of liquid; microorganism number-computing unit for computing the number of microorganisms present in liquid; solution conductivity-computing unit for computing conductivity of liquid; and warm-up section for warming up at least one of I/V amplifier and output amplifier. Warm-up section computes a warm-up signal based on the conductivity computed by conductivity-computing unit. Warm-up section computes the warm-up signal having a current the same in magnitude as that flowing through measurement electrode by using the measured solution conductivity, and applies the signal to at least one of I/V amplifier and output amplifier.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: May 6, 2014
    Assignee: Panasonic Healthcare Co., Ltd.
    Inventor: Hidenori Morita
  • Patent number: 8716026
    Abstract: Methods and systems for determining composition and completion of an experiment are provided. One-computer-implemented method for determining composition and completion of an experiment includes determining one or more characteristics of data acquired during the experiment and determining if the experiment is completed based on the one or more characteristics. One system configured to determine composition and completion of an experiment includes a processor configured to determine one or more characteristics of data acquired during the experiment and to determine if the experiment is completed based on the one or more characteristics. Another system configured to perform an experiment includes a measurement subsystem configured to acquire data during the experiment and a processor configured to determine one or more characteristics of the data during the experiment and to determine the composition of the experiment and if the experiment is completed based on the one or more characteristics.
    Type: Grant
    Filed: October 18, 2012
    Date of Patent: May 6, 2014
    Assignee: Luminex Corporation
    Inventor: Allen G. Ward
  • Patent number: 8703490
    Abstract: Histochemical process compositions comprising at least one nanoparticle in an amount effective to reduce or substantially eliminate the average number of spots per slide that result from a sample staining protocol are disclosed. Various nanoparticles, or combinations thereof, including metals, metal alloys, metal oxides, ceramics, functionalized metals or metalloids, and other miscellaneous nanoparticles, such as carbon nanoparticles and diamond nanoparticles, can be used. The nanoparticle concentration typically is from about 2 parts per million to about 20 parts per million. One embodiment of a disclosed method concerns applying a histochemical process composition to a sample, followed by performing a staining protocol on the sample. Particular embodiments concern dispensing a nanosolution onto a sample using an automated system, heating the sample, and performing a sample staining process.
    Type: Grant
    Filed: June 1, 2009
    Date of Patent: April 22, 2014
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Merrill Jackson, Michael Herman, Grace Hoben, Noemi Sebastiao, Scott Cockayne, Heather Ferrea
  • Patent number: 8700345
    Abstract: An automatic analyzer, capable of obtaining analysis results using reaction liquids with absorbance appropriate for analysis processing, is provided.
    Type: Grant
    Filed: March 19, 2009
    Date of Patent: April 15, 2014
    Assignee: Beckman Coulter, Inc.
    Inventor: Osamu Okabayashi
  • Patent number: 8691591
    Abstract: Disclosed herein are compositions and methods for combining the output obtained from redundant sensor elements in a sensor array.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: April 8, 2014
    Assignee: Trustees of Tufts College
    Inventors: David R. Walt, Todd A. Dickinson
  • Patent number: 8663575
    Abstract: There is described a sample holder and associated fluid container assembly for optical analysis of a fluid sample within a translucent container of the fluid container assembly. The sample holder includes clamping members rotatably mounted to a frame for rotation, about parallel axes spaced apart from each other, between a container accepting position in which the clamping members are spaced apart from the translucent container, and an analysis position in the clamping members abut the translucent container. The clamping members each define an optical waveguide slot extending therethrough that is substantially aligned with the translucent container when the clamping members are disposed in the analysis position, to thereby provide optical access to the translucent container for optical analysis of the fluid sample therein.
    Type: Grant
    Filed: August 31, 2010
    Date of Patent: March 4, 2014
    Assignee: Canadian Blood Services
    Inventors: Elisabeth Maurer, Georg Maurer, Keddie Brown, Gyasi Bourne, Kenneth MacCallum, Paul Charlebois
  • Patent number: 8652848
    Abstract: A method for analyzing blood cells in a whole blood sample obtained from a cat is provided. An electrical measurement result and an optical measurement result of the whole blood sample are acquired. The electrical measurement result is obtainable by electrically measuring blood cells in the whole blood sample and the optical measurement result is obtainable by optically measuring blood cells in the whole blood sample. On the basis of the electrical measurement result and the optical measurement result, volume of red blood cells in the whole blood sample is calculated.
    Type: Grant
    Filed: June 5, 2012
    Date of Patent: February 18, 2014
    Assignee: Sysmex Corporation
    Inventors: Takaaki Nagai, Hideaki Matsumoto, Yuichi Hamada
  • Patent number: 8647877
    Abstract: The present disclosure provides a nucleated red blood cell simulating particle, which may be leukocytes bound to a fluorescent-staining inhibitor capable of stably binding to the nucleus or a nucleic acid in a cell so as to reduce the binding capacity of the particles to a fluorescent dye during their detection. The present disclosure also provides a method for preparing nucleated red blood cell simulating particles, including the following steps: (a) obtaining purified leukocytes; (b) suspending the leukocytes in a cell treatment solution containing a fluorescent-staining inhibitor which stably binds to the nucleus or a nucleic acid in a cell, and (c) washing the obtained product. The present disclosure also provides a hematology control mixture containing the nucleated red blood cell simulating particles.
    Type: Grant
    Filed: December 21, 2010
    Date of Patent: February 11, 2014
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Cheng Deng, Zuyue Xu, He Liu
  • Patent number: 8617468
    Abstract: An assembly for testing platelet aggregation including an electrode subassembly that is mounted in a cuvette subassembly for use with relatively small samples containing platelets.
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: December 31, 2013
    Assignee: Chrono-Log Corporation
    Inventors: Andrew Roth, Nicholas J. Veriabo
  • Patent number: RE45539
    Abstract: A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: June 2, 2015
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: Brian L. Anderson, Bill W. Colston, Christopher J. Elkin
  • Patent number: RE45617
    Abstract: A reference control for cell by cell analysis on flow cytometric analyzers contains cellular analogs made of permeated blood cells containing therein aggregated intracellular proteins and preserved antigenic sites thereof, having cellular membrane permeable to antibodies and a suspension medium. The reference control is frozen after being prepared and thawed prior to use. The cellular analogs further contain a fluorescence marker therein. Further disclosed are a method of making the reference control and a method using the reference control, as an internal or stand-alone control, for measurements of cellular hemoglobin and cellular hemoglobin variant of a blood sample.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: July 21, 2015
    Assignee: Beckman Coulter, Inc.
    Inventors: Andreas Van Agthoven, Fabrice Malergue