Abstract: A dye development composition comprising 1,10-phenanthroline and 1,7-dihydroxynaphthalene is disclosed for use in assaying high levels of hydrogen peroxide.

Abstract: Apparatus is provided for the detection of chemiluminescence, e.g., the luminescent assay of an analyte in a sample. Sample, namely the production of a permanent photographic record of such chemiluminescence, e.g., by the use of a POLAROID film. The apparatus includes three interrelated elements. The first element comprises a film holder for holding a photographic film sensitive to a chemiluminescent reaction of the sample, the film holder defining a window in registry with any photographic film which may be held in the film holder.

Abstract: A method for the determination of peroxide in a sample is disclosed which comprises reacting the peroxide with a chromogen represented by the general formula (I) or (II) ##STR1## wherein R.sub.1 and R.sub.3 represent amino, mono- or di- substituted amino, hydroxyl or hydroxyalkyl, R.sub.4 and R.sub.5 represent hydrogen, alkyl, alkylene, acyl, halogen, sulphone, nitro, carboxyl, hydroxyl or hydroxyalkyl, R.sub.2 represents hydrogen, ##STR2## wherein R.sub.6 represents hydrogen, alkyl, aralkyl, alkylene, aryl or mono- or di- substituted aryl, and --Z-- may change to --Z.dbd. by resonance and represents --S--, --O--, --N.dbd., ##STR3## wherein R.sub.7, R.sub.8, R.sub.9 and R.sub.10 have the same significance as R.sub.6 in the presence of heme compound, iodide or bromide and measuring the absorbancy of the reaction solution in the visible ray region.

Abstract: An apparatus for measuring ozone concentration in which ozone contained in a liquid to be measured is caused to shift into a clean gas in a gas-liquid contacting tube. The gas into which the ozone has shifted is supplied to a photocell into which light is projected. The gas supplied to the photocell is exhausted through an ozone dissolving tube. The light transmitted through the photocell enters a detector. The output from the detector is utilized as an indication of the concentration of ozone in the exhausted gas, and hence in the liquid.

Abstract: The present invention is concerned with the use of aniline derivatives of the general formula: ##STR1## wherein R.sup.1 is a hydrogen atom or a --(CH.sub.2).sub.n --X radical, in which n is a whole number of from 1 to 3, X is H, OH, NH.sub.2, CH.sub.3 CONH, CH.sub.3 SO.sub.2 NH, COOH, SO.sub.3 H or --ArSO.sub.3 H, Ar being an optionally substituted arylene radical, m is a whole number of from 2 to 4, R.sup.3 is a hydrogen atom, an alkyl radical containing up to 3 carbon atoms, OCH.sub.3, CH.sub.3 CONH, COOH or SO.sub.3 H and R.sup.4 is a hydrogen, chlorine or bromine atom or a carboxylic acid or sulphonic acid group, as coupling components in oxidative color formation reactions.The present invention also provides an agent for the analytical determination of oxidizing substances by oxidative color coupling which, as coupling component, contains at least one compound of general formula (I).

Abstract: The present invention is concerned with the use of aniline derivatives of the general formula: ##STR1## wherein R.sub.1 is a hydrogen atom or a lower alkyl or aryl radical, R.sub.2 is a hydrogen atom or a lower alkyl radical which can be substituted by hydroxyl, amino, carboxyl, lower alkoxycarbonyl, lower alkanoylamido, aryl lower alkyl or aryl or by a group of the structure: ##STR2## in which R.sub.5 and R.sub.6 have the meanings given hereinafter, R.sub.3 is a hydrogen or halogen atom or a carboxyl group, R.sub.4 and R'.sub.4, which can be the same or different, are hydrogen or halogen atoms, carboxyl groups or lower alkoxy radicals or lower alkyl radicals which are preferably in the m-position and R.sub.1 and R.sub.4 or R.sub.2 and R.sub.4 or R.sub.4 and R'.sub.4, when the two substituents are ortho to one another, can together form a saturated or unsaturated hydrocarbon chain containing 2 to 6 carbon atoms which can be substituted by hydroxyl or oxo groups, R.sub.

Abstract: A method and test composition for colorimetrically determining hydrogen peroxide in a sample includes a chromogen of the formula: ##STR1## which is reacted with hydrogen peroxide in the sample in the presence of peroxidase. The absorbancy of the reaction solution is then measured.

Abstract: Disclosed herein is a method of quantitatively measuring an oxidative substance with avoiding influences of a coloring-interfering substance in the quantitative measurement of the oxidative substance by using a triphenyl methane type leuco coloring matter as a coloring reagent. Disclosed herein is also such an oxidative quantitative measurement in which the coloring sensitivity can be further adjusted. In order to avoid the influences of the coloring-interfering substance, there is employed at least one kind of (i) uricase, (ii) an anionic surface active agent and (iii) a metal chelate compound. As the triphenyl methane type leuco coloring matter, use may be made of a compound of the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent a hydrogen atom or a lower alkyl group, and X.sub.1 and X.sub.2, which may be the same as or different from each other, represent a hydrogen atom, --SO.sub.3 M.sub.1, --COOM.sub.2, --O(CH.

Abstract: A chemiluminescence method for assaying compounds containing primary amino groups using 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole fluorescers is disclosed. The 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole (CBI or CNI) is formed by reacting a compound containing a primary amino group with naphthalene-2-3-dicarboxaldehyde or anthracene-2,3-dicarboxaldehyde in the presence of cyanide ion. The reaction product of hydrogen peroxide with an oxalate ester is then combined with the CBI or CNI to form an analyte which exhibits chemiluminescence. A detector is used to measure the chemiluminescence emission from the chemiluminescence derivatized analytes. Compounds containing primary amino groups which may be assayed according to the present invention include primary amines, amino acids, peptides and catecholamines. The method can also be adapted to the analysis of trace amounts of cyanide ion and hydrogen peroxide.

Abstract: The present invention provides a process for the detection of redox reactions by introducing a redox reagent system into a test system, wherein a soluble iodate is additionally added to the test system in an amount which is in excess of the highest amount of disturbing reducing agents present in the test system.The present invention also provides a diagnostic agent for the detection of redox reactions containing a redox reagent system, wherein the test system used additionally contains an iodate which is soluble therein in an amount which is in excess of the highest amount of disturbing reducing agents present in the test system.

Abstract: The present invention provides N-acylidihydroresorufin derivatives of the general formula: ##STR1## wherein R.sup.1 is a lower alkyl, aryl or aralkyl radical, which can be substituted by carboxyl or sulphonic acid residues, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen or halogen atoms or lower alkyl or lower alkoxy radicals, Y is an --NR.sup.5 R.sup.6 or --OR.sup.7 group, wherein R.sup.5 and R.sup.6 are, in each case, hydrogen atoms or lower alkyl radicals, which can be substituted by carboxyl or sulphonic acid residues, or together represent a hydrocarbon bridge optionally interrupted by hereto atoms and R.sup.7 is a lower alkyl radical which can be substituted by a lower alkoxy or poly-lower alkoxy radical.

Abstract: An analytical element for the detection of hydrogen peroxide comprising a peroxidase-functional material, a hydrogen donor and a coupler, wherein the peroxidase-functional material and hydrogen donor are arranged so as to be separate from each other, for example in separate layers, but are made interactive by the application of a fluid sample.

Abstract: A novel imidazole derivative type reagent for analysis of hydrogen peroxide, a method of analysis of hydrogen peroxide using the same, a method of analysis of peroxidase using the same, and a multilayer chemicalanalytical element containing the same are disclosed.The imidazole derivative has the formula (I): ##STR1## wherein R.sup.1 and R.sup.2 represent an aryl group or a substituted aryl group, and R.sup.1 and R.sup.2 are identical with or different from each other, and R.sup.3 represents 2- or 3-pyrrolyl group, a substituted 2- or 3-pyrrolyl group, 2- or 3-indolyl group, or a substituted 2- or 3-indolyl group.

Abstract: A continuous process and apparatus for the analysis of a sample containing a peroxycompound in the presence of hydrogen peroxide using known colorimetric techniques, characterized in that a sample is taken continuously, is continuously mixed with a colorimetric reagent and is divided into two streams, and continuous differential colorimetric analysis of the two streams is effected to provided a differential signal, further characterized in that one such stream is heated to such a temperature that the peroxycompound reacts with the colorimetric reagent prior to effecting the differential colorimetric analysis, whereby the differential signal is a measure of the concentration of such peroxycompound. The process and apparatus may be modified in that a third stream is taken not containing any colorimetric reagent, and two differential colorimetric analyses are effected, whereby a further signal is obtained which is a measure of the concentration of the hydrogen peroxide.

Abstract: Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent lower alkyl groups, and X.sub.1 and X.sub.2 both represent --O(CH.sub.2).sub.n SO.sub.3 M in which M is a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, and n is an integer of 2-4, or either of X.sub.1 and X.sub.2 represents --O(CH.sub.2).sub.n SO.sub.3 M in which M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side.

Abstract: A particular class of triarylmethane leuco dyes are useful in analytical compositions, elements and methods. These leuco dyes are triarylmethane compounds having an aromatic heterocyclic moiety attached to the central methane carbon and which, upon interaction with hydrogen peroxide, provide dyes having a maximum absorption at a wavelength equal to or greater than about 600 nm. Particularly useful leuco dyes have the structure: ##STR1## wherein R is a 5- to 15-membered aromatic heterocyclic moiety; and R' and R" are independently open chain or cyclic amines. These compounds are useful for providing detectable dyes in compositions and elements useful for detection of hydrogen peroxide or other analytes which react to produce hydrogen peroxide in aqueous liquids, e.g. biological fluids.

Abstract: The present invention provides imidazole derivatives of the general formula: ##STR1## wherein R, R.sub.1 and R.sub.2 are defined hereinbelow. The present invention is also concerned with processes for the preparation of these imidazole derivatives and with the use thereof as redox indicators.

Abstract: A method for determination of hydrogen peroxide by chemiluminescence analysis which consists of reacting a sample containing hydrogen peroxide with an oxidizable non-fluorescent substance in the presence of an oxidizing catalyst to convert the non-fluorescent substance to a fluorescent substance, reacting the fluorescent substance with an oxalic acid di-ester and hydrogen peroxide in the presence of an inhibitor which inactivates the oxidizing catalyst, and then measuring the amount of light emission produced to determine the amount of hydrogen peroxide contained in the sample. Determination and enzyme immunoassay for a component other than hydrogen peroxide in a sample from living body and evaluation of bactericidal activity in phagocytosis of leukocytes by utilizing this method are also disclosed.

Abstract: Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent lower alkyl groups, and X.sub.1 and X.sub.2 both represent --O(CH.sub.2).sub.n SO.sub.3 M in which M is a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, and n is an integer of 2-4, or either of X.sub.1 and X.sub.2 represents --O(CH.sub.2).sub.n SO.sub.3 M in whch M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side.

Abstract: Compounds of the general formula ##STR1## wherein R represents an alkyl group of 1 to 3 carbon atoms or the group N(R.sub.2).sub.2, in which R.sub.2 represents a hydrogen atom, an alkyl group of 1 to 3 carbon atoms, or an acyl group of 1 to 3 carbon atoms, and R.sub.1 has the same meaning as R or is a hydrogen atom, are new and are suitable as chromogens for measuring H.sub.2 O.sub.2 formation in enzymatic reactions.

Abstract: Improved catalyzed substrates for use in developing characteristic colors or fluorescence in the presence of peroxidase enzymes (e.g., horseradish peroxidase) are disclosed which include as a rate accelerator a substituted phenol such as a p-halogenated phenol. The complete system typically includes a peroxide type oxidizing agent (e.g., hydrogen peroxide), a chromogenic or flurogenic compound (e.g., ABTS), a buffer and the accelerator compound. Advantageously, the accelerator should provide at least about 50 percent rate enhancement for the substrate, as compared with an otherwise identical, accelerator-free substrate reacted under the same conditions; however, the most preferred accelerator, p-iodophenol, gives enhancements on the order to 1,000 percent. The invention is particularly useful in so-called ELISA determinations which involve an enzyme-linked moiety, and permit detection at very low concentration levels unobtainable with conventional colorimetric substrates.

Abstract: Composition for the detection of(a) hydrogen peroxide or hydrogen peroxide forming substrates or(b) peroxidases or peroxidatively reacting substances which composition comprises a substrate capable of coupling and an oxidizable substance known to undergo the Trinder reaction, and(a) a peroxidase or(b) hydrogen peroxidewherein the substrate capable of coupling is a compound of the formula ##STR1## in which n and m are individually selected from whole numbers of 1 to 4,X and Y, which can be the same or different represent a valence bond or a phenylene radical,R.sub.1 and R.sub.2 are individually selected from carboxyl or sulfonic acid groups and one of R.sub.1 and R.sub.2 can also be hydrogen or lower alkyl, andR.sub.3 and R.sub.4 are individually selected from hydrogen and alkyl radicals of up to 6 carbon atoms;and/or a salt of such compound.

Abstract: A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.

Abstract: Means and method for the detection of hydrogen peroxide or of hydrogen peroxide-forming substrates, consisting of 4-aminoantipyrine and an aromatic amine or phenol and a neutral to weakly acid buffer as well as other additives and reagents which form H.sub.2 O.sub.2 with the substrate and which are known for use in Trinder's reaction, the 4-aminoantipyrine, in the immiscible state before use, being separate from the acid buffer and from the other reagents if any, and being mixed with a small amount of an alkaline buffer having a pH of 8.5 to 14.0, the two compositions being combined, in a quantity ratio to one another that is suitable for the detection test, in a single package or in a single-use unit.

Abstract: In a multilayer analysis film for detecting hydrogen peroxide by dry process which comprises a support and a reagent layer having contained therein a color indicator composition comprising peroxidase and a substance capable of causing a optically detectable change in the presence of hydrogen peroxide and peroxidase, the color indicator composition is characterized by further containing specific pyrogallol derivatives. Such pyrogallol derivatives improve stability during storage of such a multilayer analysis film.

Abstract: Apparatus for ascertaining the quantity of ozone in water and for admitting additional ozone when the ascertained quantity is insufficient has two spaced-apart electrodes which consist of an alloy having gold as the predominant constituent thereof and which can be immersed into a body of water. Each electrode is connected with a discrete voltage source by way of a discrete resistor whose resistance is substantially greater than the resistance between the electrodes when the electrodes are immersed in water. The potential difference between the electrodes is measured by a differential amplifier whose output transmits a voltage signal having an intensity proportional to the percentage of ozone in the body of water into which the electrodes are immersed.

Abstract: A luminescence detection device for quantitatively detecting an analyte in a liquid sample, which device comprises (1) a reaction compartment having, along a primary axis, opposite end portions, a first of which is for introduction of fluid reagents and sample into the compartment and the other of which forms a light transmissive aperture of predetermined size, the compartment being suitable to hold a composition which luminesces in response to contact with analyte-containing sample; (2) closure means in said first end portion for admitting a cannula, whereby fluid is introduced into said compartment, and for closing the reaction compartment; (3) a photoresponsive imaging layer; (4) means for associating the photoresponsive imaging layer and the reaction compartment such that the photoresponsive imaging layer is positioned preferably substantially perpendicular to the primary axis of the reaction compartment, at a predetermined distance from the end portion forming the aperture so as to be exposed to light em

Abstract: N-sulfoalkylaniline derivatives in which at least one sulfoalkyl of 2 to 4 carbon atoms is attached to the nitrogen atom which are useful as dyestuff forming substances, a composition for determining the presence of peroxides using such compounds and the process for determination of peroxides using such compounds.

Abstract: The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.