With Fluorescence Or Luminescence Patents (Class 436/172)
  • Patent number: 10249831
    Abstract: The present invention relates to electronic devices containing special cyclic lactams, more particularly organic electroluminescent devices, and to special cyclic lactams for use in electronic devices, more particularly in organic electroluminescent devices.
    Type: Grant
    Filed: January 11, 2016
    Date of Patent: April 2, 2019
    Assignee: Merck Patent GmbH
    Inventors: Philipp Stoessel, Amir Hossain Parham, Christof Pflumm, Anja Jatsch, Thomas Eberle, Jonas Valentin Kroeber
  • Patent number: 10132808
    Abstract: A method uses flow cytometry to prepare surface enhanced Raman scattering (SERS) substrates for obtaining SERS spectra of bacteria. The method involves using a flow cytometer to sort bacterial cells into populations of bacterial cells based upon their biophysical characteristics. The cells may then be washed with a borate buffer to remove any chemical species that degrade the SERS response. A colloid-coated bacteria suspension is then created by mixing one of the populations of bacterial cells with SERS-active colloidal particles. The colloid-coated bacteria suspension is incubated until the SERS-active colloidal particles partition through the capsule and bind to the cell wall for each bacterial cell in the colloid-coated bacteria suspension. The colloid-coated bacteria suspension is then disposed onto a filter and a SERS spectra of the colloid-coated bacteria suspension is obtained using a Raman spectrometer.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: November 20, 2018
    Assignee: The United States of America as represented by Secretary of the Navy
    Inventors: Pamela A. Boss, Kara C. Sorensen, Robert D. George, Anna Y. Obraztsova, Jonathon K. Oiler
  • Patent number: 10054546
    Abstract: A system and method for detecting an analyte includes a waveguide configured to receive a narrow-band laser signal; and a sorbent material covering an analyte detection region of the waveguide, wherein the sorbent material is configured to sorb the analyte and bring the analyte to an evanescent field of the waveguide, and wherein Raman scattering is produced by an interaction of the evanescent field and the analyte sorbed in the sorbent material along the analyte detection region of the waveguide, and the waveguide is further configured to collect the Raman scattering along the analyte detection region of the waveguide, wherein the collected Raman scattering indicates a type of the analyte.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: August 21, 2018
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Todd H. Stievater, Jacob B. Khurgin, Dmitry A. Kozak, Scott A. Holmstrom, R. Andrew McGill
  • Patent number: 10054552
    Abstract: An example method of inspecting a part includes applying a penetrant dye to the part, the penetrant dye exhibiting a fluorescent color when subjected to light from a lighting device. A portion of the part is illuminated with light from the lighting device. An image of the portion of the part is automatically recorded with a camera while the portion is illuminated. An uncertainty metric for the image is automatically determined that is indicative of a likelihood that pixels in the image having the fluorescent color represent damage to the part. At least one of the part, lighting device, and camera are automatically adjusted based on the uncertainty metric being within a predefined range. The automatic recording, determining, and adjusting steps are iteratively repeated until the uncertainty metric is greater than the predefined range, or a predefined number of iterations have been performed for the portion of the part.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: August 21, 2018
    Inventors: Sergio S. Frutuoso, Alan Matthew Finn, Gene B. Donskoy
  • Patent number: 9945848
    Abstract: One aspect relates to a method of calibrating event data. The method includes obtaining, via an electronic device including a processor, event data for an assay including a reagent. The reagent is associated with one of a plurality of manufacturing lots of the reagent. The method includes receiving one or more calibration factors for the reagent based on an identifier associated with the one of the plurality of manufacturing lots. The method further includes generating calibrated event data based on an application of the one or more calibration factors to the event data.
    Type: Grant
    Filed: February 5, 2015
    Date of Patent: April 17, 2018
    Assignee: Becton, Dickinson and Company
    Inventors: Ramez Emile Necola Shehada, Oliver Crespo-Diaz, Paul Limb
  • Patent number: 9943487
    Abstract: One aspect of the invention is a polymer material comprising a capsule coated with PDA. In certain embodiments, the capsule encapsulates a functional agent. The encapsulated functional agent may be an indicating agent, healing agent, protecting agent, pharmaceutical drug, food additive, or a combination thereof.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: April 17, 2018
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Scott R. White, Nancy R. Sottos, Sen Kang, Marta B. Baginska
  • Patent number: 9804331
    Abstract: A robust sensor, suitable for dipping into fluid wells, includes (a) a stem; (b) a whispering gallery mode (“WGM”) resonator mechanically supported by the stem; and (c) feed and pickup optical fibers optically coupled with the WGM resonator and mechanically coupled with the stem, thereby defining a stem-resonator-fiber assembly, wherein a portion of the stem-resonator-fiber assembly including the WGM resonator can fit within an imaginary cylinder having a diameter of 7 mm (or 2 mm, or even 1 mm). Such a whispering gallery mode (“WGM”) dip sensor, including (1) a stem, (2) a WGM resonator, and (3) feed and pickup optical fibers, may be made by (a) fabricating the WGM resonator and the stem from an optical fiber; (b) fabricating tapers on the feed and pickup fibers; (c) positioning tapers of the feed and pickup fibers relative to the WGM resonator such that an optical coupling between the tapers and the WGM resonator is established; and (d) mechanically coupling the stem with the feed and pickup fibers.
    Type: Grant
    Filed: August 11, 2015
    Date of Patent: October 31, 2017
    Inventors: Monica Agarwal, Natalie Huiyi Luo, Iwao Teraoka
  • Patent number: 9772485
    Abstract: A method for light-microscopy imaging of a sample structure (2, 34) is described, having the following steps: preparing the sample structure (2, 34) with markers that are transferrable into a state imageable by light microscopy, generating a sequence of individual-image data sets by sequential imaging of the sample structure (2, 34), in such a way that for each image, only a subset of the totality of the markers is in each case transferred into the state imageable by light microscopy, the markers of the respective subset having an average spacing from one another which is greater than the resolution limit of light-microscopy imaging which determines the extent of a light distribution representing one of the respectively imaged markers, generating at least two data blocks in which multiple successive individual-image data sets are respectively combined, superposing the individual-image data sets contained in the respective data block to yield a superposed-image data set, identifying an image offset between
    Type: Grant
    Filed: June 27, 2011
    Date of Patent: September 26, 2017
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Marcus Dyba, Volker Seyfried
  • Patent number: 9739770
    Abstract: Systems and methods are provided for detecting one or more particles such as individual unlabeled molecules or single nanoparticles. In examples described herein, optical energy is introduced into a microtoroid or other microcavity to generate an evanescent field. The microcavity has a functionalized outer surface that has been functionalized with a chemically or biologically active substance such as an antibody, antigen or protein. An indication of a particle bound to the functionalized outer surface of the microcavity is then detected based on a reactive interaction between the particle and the evanescent field while using frequency locking, balanced detection and various filtering techniques. The frequency locking, balanced detection and filtering techniques reduce the signal-to-noise ratio (SNR) of the detection system so that single nanoparticles (e.g. 2.5 nanometers (nm) in radius) and individual molecules (e.g. 15.5 kilo-Dalton (kDa) in size) can be detected in aqueous solution in some examples.
    Type: Grant
    Filed: March 13, 2015
    Date of Patent: August 22, 2017
    Inventor: Tsu-Te Judith Su
  • Patent number: 9733252
    Abstract: Methods and systems for plasmonically enhanced bionanoantennas for tagging, tracking, and locating targets of interest at long distances in both day and nighttime conditions. The nanoantennas are used to tag a target of interest and emit a wavelength to impart a unique biometric signature. The nanoantennas are detectable by selectively harvesting and plasmonically enhancing incident light in the visible region, then upconverting that energy through an activated phosphor.
    Type: Grant
    Filed: January 24, 2013
    Date of Patent: August 15, 2017
    Assignee: SRC, INC.
    Inventor: Adam J. Lowe
  • Patent number: 9714952
    Abstract: A sensor including a surface plasmon resonance detector with a reservoir for containing a liquid sample. The sensor further includes a sensing metallic film positioned within the reservoir so that at least a majority of a surface of the sensing metallic film is to be in contact with the liquid sample being housed within the reservoir. The sensory also includes a semiconductor device having a contact in electrical communication with the sensing metal containing film that is positioned within the reservoir. The semiconductor device measures the net charges of molecules within the liquid sample within a Debye length from the sensing metallic film.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: July 25, 2017
    Assignee: International Business Machines Corporation
    Inventors: Bobby E. Feller, Jianqiang Lin, Robert D. Miller, Ramachandran Muralidhar, Tak H. Ning, Sufi Zafar
  • Patent number: 9664686
    Abstract: An imaging system (10) and associated method for detection of protein contamination on a surgical instrument (100) that has been treated with a fluorescing stain, wherein fluorophors in the stain are capable of emitting light of an emitted type when both excited by light of an excitation type and in contact with a protein are provided. The system comprises a light tight chamber (14) for receiving the instrument (100). Inside the chamber (14) are both visible light sources (20) and excitation light sources (22) for respectively illuminating the chamber with visible and excitation type light. A digital camera (30) is able to capture a first image of the instrument (100) as illuminated by the visible light, and a second image, of patterns of fluorescence produced by the fluorophors in the stain corresponding to protein contamination. The first and second images are combined to produce a composite image of the instrument (100) highlighting the areas of protein contamination.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: May 30, 2017
    Assignee: Synoptics Limited
    Inventors: David Perrett, Nanda Kishore Nayuni, Paul Ellwood, Richard Maskell, Alasdair Hayden Wright, Sarah Thompson, Laura Sullivan
  • Patent number: 9612239
    Abstract: The invention relates to a substance determining apparatus (19) for determining a substance within a fluid. A location event determination unit (34) determines location events indicating that a particle, which may have attached the substance, is located in a sensing region and a property of the location events from the sensing signal generated by sensing the sensing region. A location events selection unit (70) selects a part of the location events having a property in a predefined property range, and a substance determination unit (40) determines the substance within the fluid (3) based on the selected part of the location events. This allows tuning the determination of the substance within the fluid to a desired property range, which can relate to a desired kind of bound particles, thereby reducing the influence of unwanted effects on the determination of the substance and, thus, improving the determination accuracy.
    Type: Grant
    Filed: February 27, 2012
    Date of Patent: April 4, 2017
    Inventors: Joannes Baptist Adrianus Dionisius Van Zon, Toon Hendrik Evers, Ron Martinus Laurentius Van Lieshout, Wilhelmina Maria Hardeman, Derk Jan Wilfred Klunder
  • Patent number: 9606062
    Abstract: The invention provides a method for detection of hazardous chemicals in a non-metallic container. The method comprises of irradiating the sample at a predefined location with an electromagnetic radiation of specific wavelength; selectively capturing a certain component of the scattered electromagnetic radiation to obtain a plurality of profiles; and filtering the profiles to obtain a signature specific to at least one hazardous chemical present in the container. The invention provides a system for obtaining a signature specific to the hazardous chemicals in the container.
    Type: Grant
    Filed: September 2, 2013
    Date of Patent: March 28, 2017
    Inventors: Siva Umapathy, Sanchita Sil, John Kiran
  • Patent number: 9556122
    Abstract: The present invention provides luminescent complexes between a lanthanide ion and an organic ligand which contains 1,2-hydroxypyridinone units. The complexes of the invention are stable in aqueous solutions and are useful as molecular probes, for example in medical diagnostics and bioanalytical assay systems. The invention also provides methods of using the complexes of the invention.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: January 31, 2017
    Inventors: Kenneth N. Raymond, Jide Xu, Evan G. Moore, Eric J. Werner
  • Patent number: 9556483
    Abstract: The present invention relates to a kit for conducting a method of measuring binding interactions using multiple sets of microparticles, wherein said set has the same identifiable characteristic and wherein one of more sets comprise subsets of microparticles and said subset presents at least one unique probe that acts as a binding partner for a target molecule in a biological sample. In particular, the invention provides for a kit for conducting a method of DNA-based tissue-typing antigens in donor tissue or recipient tissue using these multiple sets of microparticles.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: January 31, 2017
    Inventors: Jar-How Lee, Terrence Chen, Thoa Nong
  • Patent number: 9506929
    Abstract: Probes for selectively detecting compounds comprising a thiol group and an amino group (“thiols”) are disclosed. Exemplary thiols include cysteine, homocysteine, and glutathione. Embodiments of the disclosed probes produce a detectable change in absorbance spectrum and/or emission spectrum when reacted with one or more thiols in solution. Methods and kits for performing the detection also are disclosed. The probes have a general formula where each bond depicted as “” is a single or double bond; R1, R3-R6 and R8 independently are hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen; R2 is an ?,?-unsaturated aliphatic ester; R7 is oxygen, sulfur, hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen, or R7 and R8 together form a cycloalkyl or aryl ring; X1 is CH2, S, NH, O, Se, Si(CH3)2, Ge(CH3)2, Sn(CH3)2, or C(CH3)2; and X2 is CH, CH2, N, NH, or CR9 where R9 is aryl.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: November 29, 2016
    Assignee: Portland State University
    Inventors: Robert M. Strongin, Yixing Guo, Lovemore Hakuna, Mark Allen Lowry, Jorge Omar Escobedo Córdova
  • Patent number: 9458388
    Abstract: A method of correcting measurements of a chemical sensor used in an industrial facility. The method involves correcting for errors known to occur in the steady state and the dynamic state for specifically recognized situations. This method allows for correcting errors that occur due to deadtime, false zero measurements, and non-linear disturbances. The method combines automated measurement techniques and human know how to progressively learn and refine the accuracy of the corrections.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: October 4, 2016
    Assignee: Nalco Company
    Inventors: Jin Wang, Nigel P. Hilton, Glenn L. Scattergood, Sam Ferguson
  • Patent number: 9389209
    Abstract: To determine the concentration of an oxoanion in an aqueous solution, an aluminum reagent is added to the aqueous solution to form an optical analysis solution. Addition of the aluminum reagent may or may not form an alumino-oxoanion hydroxide hydrate precipitate. Light is directed into the optical analysis solution to determine an optical response of the optical analysis solution. Thereafter, the concentration of the oxoanion in the aqueous solution is determined based on the optical response of the optical analysis solution. For example, the concentration of the oxoanion may be calculated using a molar ratio relating oxoanion concentration to aluminum reagent concentration, when the aluminum reagent concentration corresponds to an inflection point of the optical response of the optical analysis solution.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: July 12, 2016
    Assignee: ECOLAB USA INC.
    Inventors: Nicholas S. Ergang, Ronald V. Davis
  • Patent number: 9341575
    Abstract: The application provides methods for the detection of an analyte in a sample by electrochemiluminescence using certain reagent compositions. Reagent compositions, reagent kits for measuring electrochemiluminescence (ECL) and electrochemiluminescence detection methods using the reagent compositions are disclosed. In particular, the application relates to the use of novel combinations of compounds, which can be used in said measurements to provide improved ECL assay performance.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 17, 2016
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Andreas Finke, Bernhard Hauptmann, Johannes Stoeckel, Michaela Windfuhr
  • Patent number: 9339813
    Abstract: The invention relates to a substance determining apparatus for determining a substance within a fluid. Particles attach the substance and bind to a binding surface (30), wherein a sensing signal is generated depending on the bound particles. Binding events indicating a binding of a particle on the binding surface (30) are determined from the generated sensing signal, and the substance within the fluid is determined based on the determined binding events. During a procedure of determining a substance within a fluid, particles may bind to the binding surface and may leave the binding surface. Therefore, during this procedure a number of binding events can be determined being much larger than the number of bound particles. The determination of the substance within the fluid can therefore be based on a very large amount of data, thereby increasing the accuracy of determining the substance within the fluid.
    Type: Grant
    Filed: December 10, 2010
    Date of Patent: May 17, 2016
    Inventors: Josephus Arnoldus Henricus Maria Kahlman, Joannes Baptist Adrianus Dionisius Van Zon, Johannes Joseph Hubertina Barbara Schleipen, Derk Jan Wilfred Klunder, Toon Hendrik Evers, Ron Martinus Laurentius Van Lieshout
  • Patent number: 9335275
    Abstract: The present invention relates to a device for identifying anomalies on medical instruments, having a data processing installation and an instrument analyzing unit. The data processing installation has a display unit, a database, a first interface and an evaluation unit and the instrument analyzing unit has a support and at least one camera. The at least one camera is arranged and oriented such that it can capture image data from medical instruments arranged on the support from at least one perspective, and the data processing installation is designed such that it uses the first interface to receive image data from the at least one camera and can store the received image data in the database. Further, it can use the evaluation unit to examine said image data for regions which have anomalies. In addition, the invention also relates to an according method.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: May 10, 2016
    Assignee: Karl Storz GmbH & Co. KG
    Inventor: Oliver Gloger
  • Patent number: 9274100
    Abstract: The instant invention refers to an optical method to extrapolate cell membrane conductance by indirect measurement of changes in transmembrane voltage, upon exposure of a cell sample to electric current pulses. The method is advantageously used for evaluating the activity of molecules able to alter, directly or indirectly, membrane permeability. A specific field of application is the screening of candidate compounds putatively acting on ion channel activity. In particular, it is open to the study of all ion channels with no limitations on the mechanisms of activation or to the ion species involved. The method is also advantageously used for evaluating a cell status, namely a differentiative or a pathologic status.
    Type: Grant
    Filed: July 16, 2010
    Date of Patent: March 1, 2016
    Inventors: Fabio Grohovaz, Stefano Pitassi, Andrea Domenico Menegon
  • Patent number: 9274008
    Abstract: A nanothermometer is disclosed. In various embodiments, a nanothermometer comprises a nanoparticle such as a gold nanoparticle, a fluorophore, and a linker, such as a peptide linker, extending between the nanoparticle and the fluorophore, whereby the fluorophore is self-quenched. The linker can comprise one or more cysteines. An unheated thermometer shows little or no fluorescence. Upon heating, fluorophore-linker conjugates are released from the nanoparticle, thereby unquenching the fluorescence. An increase in fluorescence results. In some embodiments, the increase in fluorescence can be irreversible. Methods of measuring temperature of a sample such as a biological sample, and methods of synthesizing a nanothermometer, are also disclosed. A molecular thermometer is also disclosed.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: March 1, 2016
    Assignee: Washington University
    Inventors: Mikhail Y Berezin, Tiffany P Gustafson
  • Patent number: 9266889
    Abstract: The present invention relates to substituted pterin compounds, their synthesis and use. In particular, the present invention relates to a new precursor compound and its analogs for synthesizing a new substituted pterin compound and its analogs. These new compounds are particularly suitable for treating molybdenum cofactor deficiency.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: February 23, 2016
    Assignee: Duquesne University of the Holy Ghost
    Inventors: Partha Basu, Igor Pimkov
  • Patent number: 9222941
    Abstract: The present invention provides a glucose sensor having a glucose receptor containing a binding site of formula (I): wherein X, n, m and R1 are defined herein. Also provided is a glucose sensor molecule for use in such a glucose sensor, the glucose sensor molecule containing the binding site of formula (I). The binding site has been found to have particularly good selectivity for glucose.
    Type: Grant
    Filed: January 10, 2012
    Date of Patent: December 29, 2015
    Assignee: Lightship Medical Limited
    Inventors: Nicholas Paul Barwell, Tony James
  • Patent number: 9217710
    Abstract: A fast fluorescence lifetime microscopic system images FRET between multiple labels in live cells and deep tissue, using a quantitative analysis method to reconstruct the molecular machinery behind the multiplexed FRET phenomenon. The system measures fluorescence lifetime, intensity and anisotropy as images of excitation-emission matrices (EEM) in real time and high speed within a single image scan, performs high-resolution deep-penetrating 3D FRET imaging in live samples, and fully analyzes all possible photon pathways of multiplexed FRET. The system provides a way for systematic and dynamic imaging of biochemical networks in cells, tissue and live animals, which will help to understand mechanisms of genetic disorders, cancers, and more.
    Type: Grant
    Filed: July 31, 2013
    Date of Patent: December 22, 2015
    Assignee: The Arizona Board of Regents on behalf of the University of Arizona
    Inventors: Leilei Peng, Ming Zhao
  • Patent number: 9207234
    Abstract: An optically active particle and a method of manufacturing said particles, plus methods of tagging a material of interest with said particles are disclosed. The particle comprises a surface-enhanced spectroscopy (SES) active core and a SES active reporter associated with the SES core wherein the particle produces a measurable and thermally stable SES spectrum upon optical interrogation. As used in the disclosure, thermally stable may be defined as maintaining a measurable SES spectrum after the particle or any material tagged with the particle has been exposed to a temperature substantially higher than room temperature.
    Type: Grant
    Filed: May 18, 2010
    Date of Patent: December 8, 2015
    Assignee: SICPA Holding SA
    Inventors: Marcelo Eduardo Piotti, Michael J. Natan
  • Patent number: 9199233
    Abstract: A cartridge for analyzing a biologic fluid sample is provided that includes a base plate, a sample inlet port, a first chamber wall, a second chamber wall, and an optically transparent cover panel disposed in contact with the first and second chamber walls. The base plate has a body with a chamber surface, a body passage, and a chamber entry passage. The body passage is in fluid communication with the chamber entry passage, and the chamber entry passage extends through to the chamber surface. The sample inlet port has an inlet passage in fluid communication with the body passage. The first and second chamber walls each have a height extending outwardly from the chamber surface, and the two walls are spaced apart from one another. The cover panel is sufficiently flexible to deflect and contact a central region of the chamber surface.
    Type: Grant
    Filed: March 31, 2011
    Date of Patent: December 1, 2015
    Inventor: Stephen C. Wardlaw
  • Patent number: 9158101
    Abstract: A device for distributing illumination light and detected light in a microscope includes a distributor optic configured to guide illumination light onto a sample and guide detected light proceeding from the sample onto a detector. The distributor optic includes a polarization unit disposed in a first light path and configured to convert the illumination light directed onto the sample into a first polarization state, a beam splitter disposed in the first light path and having the polarization dependence so as to guide the converted illumination light onto the sample, a first portion of the detected light back into the first light path, and a second portion of the detected light into a second light path separated from the first light path. A beam combiner is configured to combine the first portion and the second portion of the detected light and guide the first portion and second portion onto the detector.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: October 13, 2015
    Inventor: Frank Schreiber
  • Patent number: 9103720
    Abstract: Various molecular characteristics of a polymer may be determined using an integrated computational element to assay the polymer. Methods for assaying a polymer can comprise optically interacting electromagnetic radiation with a polymer and an integrated computational element; and determining a molecular characteristic of the polymer, using the integrated computational element. The molecular characteristic of the polymer may be used to determine a bulk characteristic of a fluid phase in which the polymer may be disposed.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: August 11, 2015
    Assignee: Halliburton Energy Services, Inc.
    Inventors: Aaron Gene Russell, Johanna Haggstrom, Robert P. Freese
  • Patent number: 9067207
    Abstract: Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.
    Type: Grant
    Filed: March 4, 2011
    Date of Patent: June 30, 2015
    Inventors: H. Randall Bell, John W Pettit, James P Landers, Joan M Bienvenue, Daniel J Marchiarullo, Brian E Root, Orion N Scott
  • Patent number: 9051598
    Abstract: A method of detecting the presence or absence of saccharide or saccharide level in a biological or artificial sample comprising contacting the sample with a water-soluble tetraphenylethene-cored probe having multiple functionalities of boronic acid and aggregation induced emission (AIE) characteristics, and detecting fluorescence. A method for detecting pH in a sample solution with a certain pH value comprising contacting the sample solution with a water-soluble tetraphenylethene-cored probe having multiple functionalities of boronic acid and aggregation induced emission (AIE) characteristics, and detecting fluorescence.
    Type: Grant
    Filed: May 2, 2012
    Date of Patent: June 9, 2015
    Inventors: Benzhong Tang, Yi Liu, Jingzhi Sun, Anjun Qin, Rongrong Hu
  • Patent number: 9040305
    Abstract: The invention encompasses analyzers and analyzer systems that include a single particle analyzer, methods of using the analyzers and analyzers systems to analyze samples, either for single particles, e.g., protein molecules, or for multiple particles (multiplexing), methods of doing business based on the use of the analyzers or analyzer systems of the system, and electronic media for storing parameters useful in the analyzers and analyzer systems of the invention.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: May 26, 2015
    Assignee: Singulex, Inc.
    Inventors: Philippe J. Goix, Robert Puskas, John Todd, Richard A. Livingston, Douglas Held
  • Patent number: 9040307
    Abstract: Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: May 26, 2015
    Assignee: Blood Cell Storage, Inc.
    Inventors: Michael W. Reed, Steven J. Geelhood, Robert O. Dempcy
  • Patent number: 9034655
    Abstract: Luminescent labels based on aromatic and heterocyclic compounds, including reactive intermediates used to synthesize these compounds, and methods of synthesizing and using these reporter compounds. These labels combine high photostabilities, large Stokes' shifts and contain a pyrimidinium moiety as a water-soluble group. These luminescent compounds relate generally to the following structure: The methods relate generally to the synthesis and/or use of reporter compounds for fluorescence lifetime or fluorescence polarization based applications.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: May 19, 2015
    Assignee: SETA BioMedicals, LLC
    Inventors: Leonid D. Patsenker, Inna G. Yermolenko, Iryna A. Fedyunyaeva, Yelena N. Obukhova, Olga N. Semenova, Ewald A. Terpetschnig
  • Patent number: 9029163
    Abstract: A system and method for creating a plurality of denaturation curves is disclosed. In accordance with certain embodiments, one variable, such as salt content, pH or another parameter, is varied to create a plurality of different buffer solutions. Each is then used to create a denaturation graph. The plurality of denaturation graphs allows analysis of the effect of that variable on protein stability.
    Type: Grant
    Filed: August 22, 2011
    Date of Patent: May 12, 2015
    Assignee: Avia Biosystems, Inc.
    Inventors: Richard Brown, Burleigh Hutchins, Ernesto Freire
  • Patent number: 9029167
    Abstract: This invention relates to the field of preparation technology of optical pH sensor by co-intercalated fluorescein and 1-heptanesulfonic acid sodium into layered double hydroxide. The sensor is composed by conductive materials and the surface LDH films by co-interacted FLU and HES. The synthesis method is: first: synthesis of LDH colloid suspension, subsequently, the FLU and HES co-intercalated LDH colloid solution was prepared following the ion-exchange method, then the thin film of FLU-HES/LDH was spreaded on the surface of the conductive material by electrophoretic deposition, and the oriental pH sensor was synthesized. The advantages of the present invention is: first, the LDH matrix provides chromophore molecules with a confined and stable environment; the novel electrophoretic deposition strategy in this work provides a method for precise control of thickness (ranging from nanometers to micrometers), and the oriental pH sensor show good pH responsive.
    Type: Grant
    Filed: March 12, 2010
    Date of Patent: May 12, 2015
    Assignee: Beijing University of Chemical Technology
    Inventors: Min Wei, Wenying Shi, Jun Lu, Xue Duan
  • Patent number: 9028772
    Abstract: A method may involve forming one or more photoresist layers over a sensor located on a structure, such that the sensor is covered by the one or more photoresist layers. The sensor is configured to detect an analyte. The method may involve forming a first polymer layer. Further, the method may involve positioning the structure on the first polymer layer. Still further, the method may involve forming a second polymer layer over the first polymer layer and the structure, such that the structure is fully enclosed by the first polymer layer, the second polymer layer, and the one or more photoresist layers. The method may also involve removing the one or more photoresist layers to form a channel through the second polymer layer, wherein the sensor is configured to receive the analyte via the channel.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: May 12, 2015
    Assignee: Google Inc.
    Inventors: Huanfen Yao, Jeffrey George Linhardt, Babak Parviz
  • Patent number: 9023661
    Abstract: A method for determining the presence or amount of one or more ligands or analytes in a sample including contacting the sample with a biosensor having an environmentally-sensitive dye conjugated to a binding member, wherein the biosensor compound exhibits a detectable color change as a result of binding to the ligand or analyte or as a result of a change in concentration of the ligand or analyte in the sample. The presently disclosed biosensors can be used to detect the presence of or amount of physiologically-important metabolites, such as glucose, fatty acids, and lactate, in biological samples.
    Type: Grant
    Filed: October 14, 2008
    Date of Patent: May 5, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Joseph Thomas, Michael T. Cash
  • Patent number: 9023281
    Abstract: Chemical indicator apparatuses containing one or more chemical indicators for use in monitoring the quality of water in an aquatic environment. The apparatuses are designed and configured to be submersible in the water that is being monitored. In some embodiments, each apparatus includes a plurality of immobilized-dye-based chemical indicators that undergo a physical change as levels of one or more constituents of the water change. Such indicators can be read by one or more suitable optical readers. These and other embodiments are designed and configured to be movable by a corresponding monitoring/measuring apparatus, for example, via a magnetically coupled drive. Also disclosed are a variety of features that can be used to provide a chemical indicator apparatus with additional functionalities.
    Type: Grant
    Filed: December 13, 2012
    Date of Patent: May 5, 2015
    Assignee: Step Ahead Innovations, Inc.
    Inventor: James E. Clark
  • Publication number: 20150110671
    Abstract: Rose Bengal for detecting a presence of and decomposing contaminants. A method of detecting the presence of a contaminant includes treating a substrate with Rose Bengal and exposing the substrate to a light having a wavelength within the visible spectrum. A response of the Rose Bengal is monitored during the light exposure. When a contaminant is present and is exposed to the light, a conversion of the Rose Bengal between a quinoid form and a lactone form is induced.
    Type: Application
    Filed: October 22, 2014
    Publication date: April 23, 2015
    Applicant: The Government of the United States as Represented by the Secretary of the Air Force
    Inventor: Jeffery Ray Owens
  • Patent number: 9012235
    Abstract: Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer.
    Type: Grant
    Filed: August 17, 2012
    Date of Patent: April 21, 2015
    Assignee: IntelliCyt Corporation
    Inventors: Linda Trinkle, R. Terry Dunlay, Bruce Edwards, Larry Sklar
  • Patent number: 9012234
    Abstract: A mobile water analyzing system for determining an analyte in a water sample includes a basic unit and a test element configured to be inserted into the basic unit. The test element includes a sample line with an inlet opening configured to receive the water sample, a measuring section forming a measuring track and configured to allow the determination of an analyte, a pump opening, and a key reagent disposed inside the sample line. The basic unit includes a test element receptacle configured to hold the inserted test element, an analyzer with an analyzer measuring track formed by the measuring section, and a pump actuator cooperatively connected with the pump opening.
    Type: Grant
    Filed: September 28, 2009
    Date of Patent: April 21, 2015
    Assignee: Hach Lange GmbH
    Inventors: Ulrich Lundgreen, Aria Farjam, Rolf Uthemann, Andreas Mitreiter, Isabel Huenig, Markus Lenhard, Rainer Froemel, Hans-Joachim Kumpch
  • Publication number: 20150104792
    Abstract: A fluorimetric system, apparatus and method are disclosed to measure an analyte concentration in a sample. A sensor reagent housing is also disclosed which comprises a channel and a porous medium having an analysis chemistry reagent, such as a nucleic acid analysis chemistry reagent. The apparatus and system are portable for field use, with an apparatus housing having a size adapted to fit into a user's hand. An apparatus also includes a sensing chamber and cover, a user interface, a light source; a photomultiplier tube, an amplifier, an A/D converter, a memory, and a processor. In various embodiments, the processor performs a data fitting, determines a sample reaction rate parameter, and determines the analyte concentration from a comparison of the sample reaction rate parameter with stored calibration data. The processor may also generate the calibration data and site-specific offset factors, and modify the calibration data using an offset factor.
    Type: Application
    Filed: December 22, 2014
    Publication date: April 16, 2015
    Inventors: Debapriya Mazumdar, David G. Kellner
  • Patent number: 9008471
    Abstract: Circuits and circuit elements adapted to function at optical or infrared frequencies are made from plasmonic and/or nonplasmonic particles disposed on a substrate, where the plasmonic and nonplasmonic particles have respective dimensions substantially smaller than a wavelength of an applied optical or infrared signal. Such particles are deposited on a substrate in a variety of shapes and sizes from a variety of plasmonic and/or nonplasmonic materials so as to form resistors, capacitors, inductors and circuits made from combinations of these elements.
    Type: Grant
    Filed: June 20, 2005
    Date of Patent: April 14, 2015
    Assignee: The Trustees Of The University Of Pennsylvania
    Inventors: Nader Engheta, Alessandro Salandrino, Andrea Alu
  • Patent number: 9005989
    Abstract: The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal.
    Type: Grant
    Filed: March 3, 2011
    Date of Patent: April 14, 2015
    Assignee: Massachusetts Institute of Technology
    Inventors: James Douglas Harper, Richard Hart Mathews, Bernadette Johnson, Martha Susan Petrovick, Ann Rundell, Frances Ellen Nargi, Timothy Stephens, Linda Marie Mendenhall, Mark Alexander Hollis, Albert M. Young, Todd H. Rider, Eric David Schwoebel, Trina Rae Vian
  • Patent number: 9005988
    Abstract: A method for the assessment of a multiphase (aqueous and organic) sample phase, the method comprising adding at least one detection molecule to the multiphase sample; detecting a signal emitted from the detection molecule/multiphase sample mixture, the signal being detectably different when the at least one detection molecule is present in one of either an organic phase, an aqueous phase or an interface between said organic phase and said aqueous phase of the multiphase sample; and analyzing the detected signal to assess the properties of a phase or an interface between phases. A system for use in such a method, use of at least one detection molecule for the assessment of a multiphase sample, and a composition for use in the assessment of a multiphase sample are also disclosed.
    Type: Grant
    Filed: July 20, 2009
    Date of Patent: April 14, 2015
    Assignee: Lux Innovate Limited
    Inventors: Emma Perfect, Catherine Rowley-Williams, Cameron Mackenzie, Artin Moussavi
  • Patent number: 9005910
    Abstract: The purpose is to produce, with high reproducibility, a complex of labeled probes and a carrier, said complex being to be used for detecting and measuring a target substance to be measured with high sensitivity and high stability. The means for accomplishing the purpose is that a label is bound to a probe-water soluble carrier conjugate using specific binding of an avidin compound such as avidin, streptavidin, etc. to biotin, and the binding of the avidin compound to the probe is performed before the binding to the carrier. Namely, after conjugating the avidin compound to a substance which is capable of binding to the target substance, the conjugate is bound to a high-molecule water-soluble carrier to produce a complex of the avidinized probes and the water-soluble carrier. Then the complex of the avidinized probes and the water-soluble carrier is mixed with a biotinylated label.
    Type: Grant
    Filed: April 13, 2011
    Date of Patent: April 14, 2015
    Assignees: Eiken Kagaku Kabushiki Kaisha, Advanced Life Science Institute, Inc.
    Inventors: Yoshiyuki Ohiro, Susumu Takayasu
  • Patent number: 8999725
    Abstract: In one embodiment of the present application, a stimulus-responsive gel is disclosed with optical characteristic molecule introduced therein that is capable of easily and securely converting a volume change responding to external stimulus to optical data being easy to handle in the construction of sensor system; an external stimulus measuring apparatus making use of the same; and a method of measuring external stimulus therewith.
    Type: Grant
    Filed: April 24, 2006
    Date of Patent: April 7, 2015
    Assignee: Japan Science and Technology Agency
    Inventors: Takashi Miyata, Tadashi Uragami, Kaori Okawa