Digestion Or Removing Interfering Materials Patents (Class 436/175)
  • Patent number: 10168325
    Abstract: Disclosed is a non-specific reaction inhibitor for use in an immunological measurement, comprising a complex of an antibody or a fragment of the antibody capable of specifically binding to a non-specific reaction factor, and a polymer. The non-specific reaction inhibitor can inhibit a non-specific reaction which may interfere with the accurate detection or quantification of a trace substance in an immunological measurement method.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: January 1, 2019
    Assignee: LSI MEDIENCE CORPORATION
    Inventors: Yoshikazu Takagi, Yuichi Shintani
  • Patent number: 9829431
    Abstract: A system for controlling flow of gas in a continuous flow isotope ratio analyser is provided. The system comprises gas inlet and gas outlet lines for providing gas into and from a measuring cell, and at least one switchable flow restriction on the gas inlet line, for selectively controlling gas flow into the isotope ratio analyser. Also provided is a method for determining an isotope ratio using the system according to the invention.
    Type: Grant
    Filed: April 29, 2016
    Date of Patent: November 28, 2017
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Hans-Juergen Schlueter, Eric Wapelhorst, Nils Stoebener, Hans-Juerg Jost, Tim Stoltmann
  • Patent number: 9581589
    Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: February 28, 2017
    Assignee: ANDERSON FORSCHUNG GROUP LLC
    Inventor: Norman L. Anderson
  • Patent number: 9139641
    Abstract: The present invention generally provides processes for purification of Inter-alpha inhibitor proteins (I?Ip) and compositions thereof from blood.
    Type: Grant
    Filed: May 28, 2009
    Date of Patent: September 22, 2015
    Assignee: ProThera Biologics, Inc.
    Inventors: Yow-Pin Lim, Edward S. Sirya, Peter Brne
  • Patent number: 9034657
    Abstract: A two step lateral flow assay method for identifying IgE antibodies in a sample comprises applying a sample to a sample port of a device, wherein the device is adapted to deliver the sample to a lateral flow matrix having a plurality of IgE antigen species immobilized at respective positions at a first location; allowing the sample to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species to a second location downstream of the first location; and, after a predetermined period of time, applying liquid buffer to the lateral flow matrix to mobilize labeled reagent which is adapted to bind anti-IgE antibody and which is dried on the lateral flow matrix at a location upstream of the sample port delivery of the sample to the lateral flow matrix, and allowing labeled reagent mobilized by the liquid buffer to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species and bind with any IgE antibody bound to the immobilized IgE antigen species, a
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: May 19, 2015
    Assignee: Phadia AB
    Inventors: Gerd Rundström, Per Matsson, Paul Christopher
  • Patent number: 9029165
    Abstract: A method for detecting electromagnetic waves derived from bacterial DNA, comprising extracting and purifying nucleic acids from a sample; diluting the extracted purified nucleic acids in an aqueous solvent; measuring a low frequency electromagnetic emission over time from the diluted extracted purified nucleic acids in an aqueous solvent; performing a signal analysis of the low frequency electromagnetic emission over time; and producing an output, based on the signal analysis, in dependence on the DNA in the sample. The DNA may be extracted from at least one of blood, feces, urine, saliva, tears, seminal fluid, sweat, seminal and vaginal fluids of a patient, or water to determine, e.g., potability. The samples may be frozen. The extracting and purifying may comprise diluting the sample with an aqueous buffer and mixing; degrading proteins in the diluted sample; precipitating DNA from the buffer solution; and resuspending the precipitated DNA in an aqueous solution.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: May 12, 2015
    Inventor: Luc Montagnier
  • Patent number: 9029162
    Abstract: Disclosed are methods and systems for the analysis of testosterone in a sample using supported liquid extraction and liquid chromatography-mass spectrometry.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: May 12, 2015
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Russell Philip Grant, Matthew Crawford, Donald Walt Chandler, William Curtin
  • Patent number: 9018017
    Abstract: Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample such as a whole blood sample with one or more leukocidal reagents that reduce or eliminate the metabolic activity of leukocytes, and performing an immunoassay on the amended sample to determine the concentration of analyte in the sample. Preferably, the sample is amended with one or more enzymes and optionally one or more enzyme substrates and cofactors.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: April 28, 2015
    Assignee: Abbott Point of Care, Inc.
    Inventors: John Lewis Emerson Campbell, Graham Davis, John Emegbero Omakor, Adam Roger Moss, Ganesh Guhados
  • Patent number: 8993341
    Abstract: Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g, compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (PVP) to form PVP-assay inhibitor complexes, and spin filtration to separate the PVP-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition.
    Type: Grant
    Filed: May 11, 2012
    Date of Patent: March 31, 2015
    Assignee: Exact Sciences Corporation
    Inventors: Janelle J. Bruinsma, Hemanth D. Shenoi, Michael J. Domanico, James P. Light, II
  • Publication number: 20150056715
    Abstract: Methods and systems for acoustically treating material using an acoustic energy system having a movable outer surface that contacts a sample holder. The outer surface may be cylindrical and rotate about a central axis, e.g., so that a sample holder may be driven to move by the outer surface. Acoustic energy may be emitted from within the outer surface to a treatment area outside of, and near, the outer surface. Thus, a sample holder in contact with the outer surface may have a sample exposed to acoustic energy while rotation of the outer surface may move the sample holder relative to treatment area. One or more additional rollers or other components may bias the sample holder into contact with the outer surface, to e.g., so the sample holder is squeezed between the outer surface and a roller or other biasing component.
    Type: Application
    Filed: August 20, 2014
    Publication date: February 26, 2015
    Applicant: Covaris, Inc.
    Inventors: James A. Laugharn, JR., Xiaoyin He
  • Patent number: 8962343
    Abstract: The invention relates to a process and a device for parallel fractionating a multiplicity of individual samples (29) within a 3-dimensional separation medium (6), with the subsequent process steps being carried out: A first space (2) which extends essentially across all three space coordinates contains the separation medium (6). A multiplicity of individual samples (29) is arranged close to an interface of the first space (2), with the individual samples (29) being arranged essentially in a planar fashion, i.e. their particular center-of-gravity positions are described by two coordinates. Under the influence of one or more physical parameters, the individual samples (29) are able to migrate essentially perpendicularly to the area of their application through the separation medium (6), and are fractionated in the process according to one or more of their properties.
    Type: Grant
    Filed: March 5, 2003
    Date of Patent: February 24, 2015
    Inventors: Robert Alexander Ventzki, Josef Stegemann
  • Patent number: 8932864
    Abstract: Provided is a method and device for identifying glycated protein in a sample using a matrix including a boronic acid moiety that binds glycated proteins.
    Type: Grant
    Filed: March 18, 2013
    Date of Patent: January 13, 2015
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Sang-kyu Kim, Sung-ha Park, Kyung-mi Song, Soo-suk Lee, Youn-suk Choi
  • Patent number: 8933396
    Abstract: Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.
    Type: Grant
    Filed: July 7, 2011
    Date of Patent: January 13, 2015
    Assignee: Thermo Fisher Scientific GmbH
    Inventors: Joel Louette, John Charles Rogers, Scott M. Peterman, Bruno Domon, Elodie Duriez
  • Patent number: 8927292
    Abstract: The presently disclosed subject matter provides methods for evaluating and characterizing peptides, peptide mixtures, and polypeptide mixtures. More particularly, the presently disclosed subject matter provides methods for evaluating or characterizing complex peptide or polypeptide mixtures comprising glutamic acid, alanine, tyrosine, and lysine, e.g., Copolymer-1 or glatiramer acetate, including, but not limited to, methods of identifying, isolating, quantifying, and purifying amino acids, peptides, polypeptides, and combinations thereof having a diethylamide group instead of a carboxyl group present on the C-terminus. The presently disclosed methods can be used to determine the mole percent of polypeptides having a diethylamide group at a C-terminus thereof and can be used to evaluate one or more properties of a sample of one polypeptide mixture as compared to one or more properties of a different sample of a polypeptide mixture.
    Type: Grant
    Filed: September 6, 2013
    Date of Patent: January 6, 2015
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventors: Zachary Shriver, Ganesh V. Kaundinya, Jonathan C. Lansing, Corinne Bauer, Xiangping Zhu
  • Patent number: 8927290
    Abstract: Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.
    Type: Grant
    Filed: February 16, 2011
    Date of Patent: January 6, 2015
    Assignee: One Lambda, Inc.
    Inventors: Adam Idica, Chun-Tsan Deng, Paul I. Terasaki
  • Patent number: 8921116
    Abstract: The presently disclosed subject matter provides methods for evaluating and characterizing peptides, peptide mixtures, and polypeptide mixtures. More particularly, the presently disclosed subject matter provides methods for evaluating or characterizing complex peptide or polypeptide mixtures comprising glutamic acid, alanine, tyrosine, and lysine, e.g., Copolymer-1 or glatiramer acetate, including, but not limited to, methods of identifying, isolating, quantifying, and purifying amino acids, peptides, polypeptides, and combinations thereof having a diethylamide group instead of a carboxyl group present on the C-terminus. The presently disclosed methods can be used to determine the mole percent of polypeptides having a diethylamide group at a C-terminus thereof and can be used to evaluate one or more properties of a sample of one polypeptide mixture as compared to one or more properties of a different sample of a polypeptide mixture.
    Type: Grant
    Filed: June 24, 2013
    Date of Patent: December 30, 2014
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventors: Zachary Shriver, Ganesh V. Kaundinya, Jonathan C. Lansing, Corinne Bauer, Xiangping Zhu
  • Patent number: 8921119
    Abstract: A method of conducting microwave-assisted high pressure high temperature chemistry is disclosed. The method includes the steps of digesting a sample in a strong acid at a temperature of at least 200° C. in a pressure resistant vessel that includes a lid while exerting a defined force against the lid in order to maintain gases under pressure in the vessel; directing gas under excess pressure from the vessel into a circumferential passage defined by the vessel and its pressure resistant lid; and directing the gas from the circumferential passage outwardly from the lid while preventing gas from flowing outwardly over the edge of the vessel.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: December 30, 2014
    Assignee: CEM Corporation
    Inventors: Wyatt Hargett, Jr., David Barclay
  • Publication number: 20140377880
    Abstract: An apparatus for preparing samples for chemical analysis includes a container receptacle for receiving a sample container having a crucible portion and an expansion portion. The container receptacle includes a heating compartment and a cooling compartment spaced apart from the heating compartment. The heating compartment is shaped to receive the crucible portion of the sample container, and the cooling compartment is shaped to receive the expansion portion of the sample container. The apparatus also includes a heating mechanism for heating the sample within the crucible portion of the sample container, a first cooling mechanism for cooling the expansion portion of the sample container, and a second cooling mechanism for cooling the crucible portion of the sample container.
    Type: Application
    Filed: June 18, 2014
    Publication date: December 25, 2014
    Inventors: Ron J. Emburgh, Ravi K. Kanipayor
  • Publication number: 20140363896
    Abstract: When the type is to be changed from serum (preceding sample) to urine (current sample), “serum” is set to a preceding type and “urine” is set to a measurement type at number 1 in a condition number. At condition number 1, the wash type is pattern 1, with washing performed once with detergent 1. Where the preceding sample is serum and the current sample is CSF, the condition number is 2 and the wash type is pattern 2, with washing performed twice using detergent 1 and once with detergent 2. Where the preceding sample is urine and the current sample is CSF, the condition number is 3 and the wash type is pattern 3, with washing performed once with detergent 1, once with detergent 2, and once with water. In the case of pattern 4, washing is performed three times with detergent 1.
    Type: Application
    Filed: December 14, 2012
    Publication date: December 11, 2014
    Inventors: Naoto Suzuki, Yoshiaki Saito, Yoichi Aruga, Toshihide Orihashi, Kazuhiro Nakamura
  • Patent number: 8835125
    Abstract: Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: September 16, 2014
    Assignee: Affymetrix, Inc.
    Inventors: Robert G. Kuimelis, Glenn H. McGall, Stephen P. A. Fodor
  • Patent number: 8828738
    Abstract: The invention is directed to methods and devices for reducing interference from heterophile antibodies in an analyte immunoassay. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with non-human IgM or fragments thereof by dissolving into said sample a dry reagent to yield a non-human IgM concentration of at least about 20 ?g/mL or equivalent fragment concentration; and (b) performing an electrochemical immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG or fragments thereof in addition to the IgM of fragments thereof.
    Type: Grant
    Filed: December 6, 2011
    Date of Patent: September 9, 2014
    Assignee: Abbott Point of Care Inc.
    Inventors: John Lewis Emerson Campbell, John Emegbero Omakor
  • Patent number: 8785203
    Abstract: The invention provides reagents, methods and kits for detection of proteins and quantitative determination of protein concentration. The reagents comprise a protein-complexing dye, such as a Coomassie dye and one or more dextrins, for the elimination of interference caused by detergents.
    Type: Grant
    Filed: October 21, 2011
    Date of Patent: July 22, 2014
    Assignee: Expedeon Limited
    Inventors: Daniel Brian Jones, Heikki Lanckriet
  • Patent number: 8778277
    Abstract: The invention provides reagents, methods and kits for detection of proteins and quantitative determination of protein concentration. The reagents comprise a protein-complexing dye, such as a Coomassie dye and one or more dextrins, for the elimination of interference caused by detergents.
    Type: Grant
    Filed: April 30, 2007
    Date of Patent: July 15, 2014
    Assignee: Expedeon Limited
    Inventors: Daniel Brian Jones, Heikki Lanckriet
  • Patent number: 8778688
    Abstract: Described herein are high-throughput methods of monitoring D-serine levels in plasma. The assay involves the use of strong cation solid phase extraction (SPE) to isolate D-serine from plasma, followed by quantitation of D-serine using the D-amino acid oxidase- (DAAO-) catalyzed reaction. Also described are methods of screening for compounds that act as DAAO inhibitors.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: July 15, 2014
    Assignee: The Johns Hopkins University
    Inventors: Barbara S. Slusher, Jesse Alt, Camilo Rojas, Takashi Tsukamoto
  • Patent number: 8765391
    Abstract: A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: July 1, 2014
    Assignee: Zyomyx, Inc.
    Inventors: Frank Zaugg, Renee Tobias, Silvia McManus-Munoz, Peter Kernen, Laurence Ruiz-Taylor, Peter Wagner
  • Patent number: 8753888
    Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: June 17, 2014
    Assignee: The General Hospital Corporation
    Inventors: Denise L. Faustman, Douglas E. Burger
  • Patent number: 8753836
    Abstract: Dewaxing buffers containing a water-soluble organic solvent, methods for use thereof, and kits incorporating dewaxing buffers. The dewaxing buffers contain a water soluble organic solvent that has a boiling point of at least 80° C. The dewaxing buffers described herein can be used to quickly and efficiently remove embedding paraffin from slide-mounted tissue sections in an aqueous buffer solution at elevated temperature without fear of paraffin redeposition of the slides. In addition, the dewaxing buffers described herein can be used to perform dewaxing and HIER in a single step, which can substantially reduce protocol time and lower the risk of sample loss or damage.
    Type: Grant
    Filed: January 23, 2012
    Date of Patent: June 17, 2014
    Assignee: Richard-Allan Scientific Company
    Inventors: Jennifer H. Freeland, Marsha A. Harvey
  • Patent number: 8722337
    Abstract: The instant invention provides an intergrated kit with solutions and instruments to isolate various biological samples from biological samples. The kit enables an user to preserve specimens and isolate biomolecules with features and benefits of high quality, easy, fast, no toxicity, safe to user and environment, low demanding, cost-effective, reducing waste, saving nature resources and protecting environment, and leads to a low-carbon and Green economy in preparation of specimens. The integrated kit enables the user to extract biomolecules including DNA/ccfDNA, Large RNA/mRNA/ccfRNA, Small RNA/miRNA/ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite using one seamless procedure.
    Type: Grant
    Filed: August 6, 2013
    Date of Patent: May 13, 2014
    Assignee: BexMart
    Inventors: Bob Han, Eric Han, Xiaohui Xiong, Xiaoliang Han
  • Patent number: 8715951
    Abstract: Disclosed are antibodies that selectively bind to blood coagulation factor FVIII, and highly sensitive immunological assays comprising these antibodies. Preferred assays can detect FVIII at about 3500-fold below the normal physiological levels, and have a wide array of applications including accurate monitoring of FVIII concentration in pharmaceutical products for treatment of blood coagulation disorders, and determination of FVIII levels in plasma of human patients, including those with blood coagulation disorders such as hemophilia.
    Type: Grant
    Filed: July 18, 2012
    Date of Patent: May 6, 2014
    Assignee: The University of Vermont and State Agriculture College
    Inventors: Behnaz Parhami-Seren, Kenneth G. Mann, David N. Fass
  • Patent number: 8703492
    Abstract: An automated sample processing system having a sample input adapted to simultaneously receive a number of sample containers, a reagent input adapted to receive one or more new reagent supplies, a consumable input adapted to receive one or more new consumable supplies, a solid waste output adapted to receive used consumable supplies, a liquid waste output adapted to receive one or more used reagent supplies, and a processing center. The processing center includes a decapper adapted to remove a lid from at least one sample container, an aspirator adapted to remove a specimen from the at least one sample container and transfer the specimen to an output vessel, and a capper adapted to replace the lid on the at least one sample container.
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: April 22, 2014
    Assignee: Qiagen Gaithersburg, Inc.
    Inventors: Brian Austin Self, Jonathan Matthew Miller, Carl Theodore Edens
  • Patent number: 8679856
    Abstract: Methods and apparatus are described for the processing (for example washing, incubation, etc.
    Type: Grant
    Filed: March 26, 2007
    Date of Patent: March 25, 2014
    Assignee: Silicon Biosystems S.p.A.
    Inventor: Nicolò Manaresi
  • Patent number: 8669117
    Abstract: Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from incorporation of either one or two 18O atoms. The outcome is accurate quantification of isotopically labeled peptides.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: March 11, 2014
    Assignee: University of North Dakota
    Inventors: Masaru Miyagi, Chandra sekhar rao Kadiyala
  • Patent number: 8652771
    Abstract: The present invention relates in general to the discovery of urinary succinate as a novel biomarker of kidney disease. More specifically, the invention provides for the measurement of succinate in urine samples that has great potential for the easy and early diagnosis of kidney damage and would allow early prediction of kidney disease and therapeutic intervention.
    Type: Grant
    Filed: May 27, 2009
    Date of Patent: February 18, 2014
    Assignee: University of Souther California
    Inventor: Janos Peti-Peterdi
  • Patent number: 8642348
    Abstract: Provided are efficient, cost-effective and water tolerant methods (e.g., single-vial methods) for preparing fatty acid esters from organic matter, including: obtaining organic matter having at least one fat substituent, contacting the organic matter in a reaction mixture with a basic solution under conditions suitable to provide for hydrolytic release of monomeric fatty acids from the at least one fat substituent to provide a base-treated reaction mixture, and esterifying the monomeric fatty acids of the base-treated reaction mixture by acidification of the reaction mixture and treating in the presence of an organic alcohol to provide fatty acid esters. The methods optionally further include, prior to esterifying, neutralizing the base-treated reaction mixture to provide for neutralized fatty acids, separating the neutralized fatty acids from the neutralized reaction mixture, and dissolving the separated fatty acids in the esterification reaction mixture.
    Type: Grant
    Filed: January 10, 2008
    Date of Patent: February 4, 2014
    Assignee: Washington State University
    Inventors: James V. O'Fallon, Charles T. Gaskins, Jan R. Busboom, Mark L. Nelson, Brian J. Kraft
  • Patent number: 8637327
    Abstract: The invention relates to a method for optimizing the automatic fluorescence pattern recognition in immunodiagnosis. In this method, in addition to or together with the fluorescence dye, one or more other indicator dyes for the identification of relevant structures are incubated before an image is taken with a camera.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: January 28, 2014
    Assignee: Euroimmun Medizinische Labordiagnostika AG
    Inventors: Stöcker Winfried, Hendrik Fauer, Christopher Krause, Erhardt Barth, Thomas Martinetz
  • Patent number: 8633032
    Abstract: A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells that each store at least a reagent, a sample well into which a biological sample is introduced, a waste liquid well, and a collection well in which an extracted nucleic acid is collected, and an extraction filter cartridge equipped with an extraction filter for separating and extracting a nucleic acid from the biological sample, wherein the extraction filter cartridge is formed in a manner that enables the extraction filter cartridge to be supported on the waste liquid well and the collection well.
    Type: Grant
    Filed: February 19, 2013
    Date of Patent: January 21, 2014
    Assignee: Toppan Printing Co., Ltd.
    Inventors: Shuichi Akashi, Ryoichi Asai
  • Patent number: 8628932
    Abstract: The invention is directed to methods of screening for HLA antibodies comprising detecting antibodies specific for native HLA antigens and denatured HLA antigens. The invention also provides for methods of removing antibodies specific for denatured HLA antigens or antibodies specific for native HLA antigens from a serum sample. In addition, the invention also provides for method of predicting whether a transplant recipient has an increased risk for rejecting the transplanted organ.
    Type: Grant
    Filed: March 9, 2011
    Date of Patent: January 14, 2014
    Assignee: One Lambda
    Inventors: Jar-How Lee, Rui Pei, Thoa Nong
  • Patent number: 8603828
    Abstract: Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.
    Type: Grant
    Filed: November 8, 2010
    Date of Patent: December 10, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Roger Walker, Benedicte Jardin
  • Patent number: 8603773
    Abstract: Methods, systems, and computer program products for the analysis of a blood sample are disclosed. One embodiment is a method of detecting and enumerating hard-to-ghost cells in a blood sample. Another embodiments is a method of analyzing reticulocytes in a blood sample. Methods of using blood count parameters are also provided.
    Type: Grant
    Filed: March 24, 2010
    Date of Patent: December 10, 2013
    Assignee: Beckman Coulter
    Inventors: Christophe P. Godefroy, John Steven Riley, Patricio J. Vidal
  • Patent number: 8603830
    Abstract: The present invention relates to a method for the determination of polysorbate in a protein-containing sample. The method of the present invention involves the pretreatment of the sample by alkaline hydrolysis followed by colorimetric determination.
    Type: Grant
    Filed: March 1, 2012
    Date of Patent: December 10, 2013
    Assignees: Baxter International Inc., Baxter Healthcare SA
    Inventors: Alfred Weber, Andrea Engelmaier, Heinz Anderle, Hans-Peter Schwarz
  • Patent number: 8597954
    Abstract: The present invention relates to a stool sample processing method and stool sample processing container the a stool sample processing container provided with a stool collection tool, a suspending solution holding portion and a processing solution holding portion, wherein stool sample preparation solutions consisting of a suspending solution and a stool sample processing solution are respectively housed in a suspending solution holding container and a processing solution holding container, after first mixing a stool sample with the suspending solution and suspending therein, a sealant is released into the suspending solution holding container by pressing on the lower portion of the processing solution holding container, and the resulting stool suspension mixes with the stool sample processing solution that stabilizes the nuclide acid.
    Type: Grant
    Filed: November 11, 2010
    Date of Patent: December 3, 2013
    Assignee: Olympus Corporation
    Inventors: Yasuo Tanigami, Kazue Nakajima, Takami Shibazaki
  • Patent number: 8586356
    Abstract: The present invention provides methods of evaluating CHO cells.
    Type: Grant
    Filed: January 22, 2009
    Date of Patent: November 19, 2013
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventors: Carlos Bosques, Jennifer Murphy, Hetal Sarvaiya, Nathaniel Washburn, Cuihua Liu, Xiao-Jin Xu
  • Patent number: 8586367
    Abstract: The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites including extracting a biological sample with a extraction buffer containing a phase separator and a volatile neutral ammonium salt under conditions which allow for immediate disruption of cells in the biological sample, separating the polar metabolites in the extract by chromatography, and analyzing the separated polar metabolites. Moreover, a method for quenching a biological sample containing cellular material is contemplated.
    Type: Grant
    Filed: July 7, 2010
    Date of Patent: November 19, 2013
    Assignee: BASF Plant Science Company GmbH
    Inventors: Gerd Balcke, Tilmann B. Walk, Martin Dostler, Ralf Looser, Susan Carvalho
  • Patent number: 8580531
    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: November 12, 2013
    Assignee: Bio-Rad Innovations
    Inventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
  • Patent number: 8580530
    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies of an individual, comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes, erythrocyte membrane fragments or blood group antigens.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: November 12, 2013
    Assignee: Bio-Rad Innovations
    Inventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
  • Patent number: 8574913
    Abstract: Hemoglobin in a sample solution is quickly and reliably denatured; at the same time, quick and accurate measurement of hemoglobin and a hemoglobin derivative is realized. In a method for measuring hemoglobin and a hemoglobin derivative, and a reagent composition, a measurement kit, an analysis device, and an analysis system used in the method, a sample solution containing a blood component is treated with a nonionic surfactant, an oxidizing agent, and a metal salt to denature hemoglobin in the sample solution to measure the hemoglobin, and thereafter the amount of a hemoglobin derivative in the sample is measured by an immunological method using an antibody specifically binding to a denatured site of the denatured hemoglobin derivative.
    Type: Grant
    Filed: October 29, 2008
    Date of Patent: November 5, 2013
    Assignee: Panasonic Corporation
    Inventors: Hirotaka Tanaka, Masanori Tanaka
  • Patent number: 8557590
    Abstract: A method for measuring glycated hemoglobin includes hemolyzing a blood sample with a hemolysate; reacting the hemolyzed blood sample with bead conjugates in which beads are conjugated with glycated hemoglobin binding materials; measuring the amount of total hemoglobin in the reacted blood sample; isolating normal hemoglobin from the glycated hemoglobin conjugated with the bead conjugates; measuring the amount of glycated hemoglobin isolated from the normal hemoglobin; and determining the percentage of the glycated hemoglobin in the blood sample on the basis of the measured amounts of total hemoglobin and glycated hemoglobin. The isolation of normal hemoglobin is performed by absorbing the normal hemoglobin using an absorption pad that is a porous pad having pores, each of which has a size greater than the size of the normal hemoglobin and smaller than the size of the bead.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: October 15, 2013
    Assignee: Infopia Co., Ltd.
    Inventors: Byeong-Woo Bae, Sung-Dong Lee, Hyong-Soo Kim
  • Patent number: 8557593
    Abstract: The invention relates to the detection of DHA and EPA. In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry.
    Type: Grant
    Filed: September 15, 2011
    Date of Patent: October 15, 2013
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Scott M. Goldman, Julie A. Neidich
  • Patent number: 8557531
    Abstract: Endothelial cells are detected in a blood sample by enriching the endothelial cells from the blood sample followed by performing on the enriched endothelial cells an immunoassay capable of detecting antigens expressed by the endothelial cells. The immunoassay is capable of detecting antigen expressed from 300 endothelial cells per milliliter of blood. The method can be used for assaying mature circulating endothelial cells or circulating endothelial progenitor cells.
    Type: Grant
    Filed: April 18, 2007
    Date of Patent: October 15, 2013
    Assignee: Wellstat Biologics Corporation
    Inventor: Robert M. Lorence
  • Patent number: 8536312
    Abstract: The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The inventive monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: September 17, 2013
    Assignee: Adnagen GmbH
    Inventors: Christiane Hollmann, Silke Zimmermann, Stefan Stachelhaus, Winfried Albert