Digestion Or Removing Interfering Materials Patents (Class 436/175)
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Patent number: 7410753Abstract: The present invention provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides reagents for use in an automated environment for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample.Type: GrantFiled: March 21, 2003Date of Patent: August 12, 2008Assignee: Ventana Medical Systems, Inc.Inventors: Keri Hopkins, Alexis Pusch, Catherine Wolf, Anthony Hartman, James Pettay, Kimberly Christensen, Ethel Macrea, Noemi Sebastiao
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Patent number: 7405036Abstract: The present invention is directed towards an apparatus and method for disinfecting biological samples, including semen and the like, and for removing a biological sample pellet from surrounding media while reducing the risk of recontamination of the pellet with said media. An insert receptacle includes a receiving cylinder having a drain in a floor which allows the fluids to decant slowly down the side of a tube. The insert receptacle further includes an aspiration channel which allows an aspiration device, such as a modified micro-pipette, to be utilized for extracting a sample pellet from the bottom of the tube. Utilizing the novel apparatus, a method for reducing pathogens in a biological sample includes decanting the biological sample through the receiving cylinder and into a tube where it passes through a multi-layer gradient including an enzyme suitable for removal of at least one pathogen from the biological sample.Type: GrantFiled: July 26, 2004Date of Patent: July 29, 2008Assignee: Safety Art, LLCInventors: Naida M. Loskutoff, Kari Morfeld, James Dow
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Patent number: 7405047Abstract: The present invention relates to the estimation of the amount of subtypes of specific cells, for example the number of certain subtypes of leukocytes, by measurements of unique proteins in extracts of blood and other biological material. The knowledge of the number or amount of specific subtypes of white cells is important in the clinical diagnosis and surveillance of subjects with inflammatory disease including infectious disease, cancer, allergy/asthma etc.Type: GrantFiled: June 24, 2002Date of Patent: July 29, 2008Assignee: Phadia ABInventor: Per Venge
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Patent number: 7405083Abstract: The subject invention provides a sample processing technique for purifying a biological or chemical sample. The invention is particularly well-suited to prepare a sample for mass spectrometry. The process is to be performed in an article having at least one well, in which the surface of the well is at least partially hydrophobic and/or modified with bio-specific ligands. Targeted solutes, such as salts or small molecule contaminants, can be removed from a solution to allow for a purified solution of a desired type of solute, such as peptides and/or proteins.Type: GrantFiled: May 13, 2004Date of Patent: July 29, 2008Assignee: Becton, Dickinson and CompanyInventor: Xiaoxi (Kevin) Chen
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Publication number: 20080166820Abstract: An extraction method and apparatus is provided for obtaining quick, safe and highly sensitive testing of any of a variety of body fluids including saliva, blood, urine or other fluids for drugs of abuse or other analytes. The apparatus includes a latchable extraction wand for obtaining body fluid samples from a subject which is adapted to maximize the portion of the body fluid sample that will go into a graduated bottle containing a buffer solution, and a testing device wherein the sample will be received and into which test strips can be inserted to determine levels of drugs of abuse or other analyte in the sample. In one of the methods of the invention, energy is imparted to the sample and buffer solution, such as by shaking, and this facilitates the reduction of sample viscosity, such as by promoting the breakdown of mucins when the sample is saliva.Type: ApplicationFiled: January 9, 2007Publication date: July 10, 2008Inventors: Martin Gould, Robert Smalley, Robert Bernstine, Jackie Gale, John Donovan
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Patent number: 7396688Abstract: The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.Type: GrantFiled: December 14, 2004Date of Patent: July 8, 2008Assignee: Genencor International, Inc.Inventors: David A. Estell, Grant C. Ganshaw, Christian Paech, Sigrid Paech
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Patent number: 7390677Abstract: An immunoassay comprises the steps of: (a) mixing a whole blood sample with sensitized insoluble carrier particles smaller than erythrocytes to cause an immune agglutination reaction; (b) introducing the resulting immune agglutination reaction mixture including agglutinated particles and unagglutinated particles to a flow cell, irradiating the particles passing through the flow cell with laser light, and detecting scattered lights generated thereby; (c) setting a threshold value for distinguishing unagglutinated particles from agglutinated particles and a threshold value for distinguishing the agglutinated particles from blood cells with regard to intensity of the scattered light; and (d) distinguishing and counting the unagglutinated particles, the agglutinated particles and the blood cells from the scattered lights detected in the step (b), in reference to the threshold values set in the step (c).Type: GrantFiled: June 11, 2001Date of Patent: June 24, 2008Assignee: Sysmex CorporationInventors: Kazuhiro Nakashima, Tsuneyoshi Torii, Hiroshi Tsuchiya, Shinya Uchida, Aya Konishi, Kunio Tanaka
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Patent number: 7384791Abstract: A method of analyzing blood includes delivering a blood sample to a test device, applying a spatially varying electric field to the blood sample to provide a depleted cell concentration in a portion of the blood sample, and sensing a property of the portion of the blood sample.Type: GrantFiled: January 21, 2004Date of Patent: June 10, 2008Assignee: Hewlett-Packard Development Company, L.P.Inventors: David Tyvoll, Winthrop D. Childers, Kirk Norton, Bryan J. Johnson
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Publication number: 20080131975Abstract: A device and method for the removal of excess embedding tissue from histological samples comprising a plate with heating elements. The plate has at least one trench and a plurality of grooves at the surface of the plate. The plate is arranged at a slight angle as compared to a horizontal plane. Once the device reaches working temperature the user slide the cassette, with extra embedding medium down the surface of the plate. The extra embedding medium melts from the cassette and runs through the plurality of grooves to the trench and finally to a receptacle.Type: ApplicationFiled: October 30, 2007Publication date: June 5, 2008Inventors: Jeffrey Allan Ross, Carolyn Ann Durkowski
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Patent number: 7374951Abstract: A system and an apparatus for use in detecting a target microorganism or agent is disclosed which involves a solid support carrying a binding partner specific for the particular microorganism or agent and the solid support being characterised in that it defines means for protecting the binding partner from being dislodged or scraped off the solid support by physical means. The provision of protection against the binding partner being dislodged from or scraped off the solid support improves the reliability of tests such as immunoassays being conducted with the solid support and also enables such tests to be automated. Modules and machines for use with the solid support, and the automated conduct of tests ate also disclosed.Type: GrantFiled: October 7, 2002Date of Patent: May 20, 2008Assignee: TECRAInternational Pty Ltd.Inventors: Megan Ash, David William Edwards, Aaron Peter Gibbeson, Dianne Barbara Kerr, Lisa Frances Moncur, Philip Albert Myers
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Patent number: 7361479Abstract: The present invention provides a method of determining the presence or amount of newly synthesized antibody in a sample in response to an immunogen by detecting the released antibodies or parts thereof in a sample containing lymphocytes which have been disrupted whereby to release the synthesized antibodies or parts thereof associated with said lymphocytes whereby to determine the presence or amount of newly synthesized antibody in said sample, methods of diagnosis using said method and kits for performing the method. The method may also be modified to allow detection of non-specific infection indicators.Type: GrantFiled: June 14, 2000Date of Patent: April 22, 2008Assignee: PlasmAcute ASInventor: Lars Reinhardt Haaheim
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Patent number: 7361513Abstract: Disclosed are cellular hemoglobin A1c (Hb A1c) normal and abnormal (high) controls for use in detecting Hb A1c levels. The present invention also relates to methods for generating cellular Hb A1c controls using red blood cells and methods for using the cellular controls. The present invention encompasses several methods for the preparation of Hb A1c cellular controls including: (1) a boronate method where the glycation occurs non-specifically; (2) a stabilized diabetic blood method where the glycation occurs specifically on Hb A1c, and (3) the glycation of normal blood method that is achieved by controlling conditions such that glycation occurs predominantly on Hb A1c. These methods produce cellular Hb A1c controls with desirable stability and that can be detected on a variety of instruments.Type: GrantFiled: April 8, 2005Date of Patent: April 22, 2008Assignee: Streck, Inc.Inventors: Wayne L. Ryan, Jiong Wu
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Patent number: 7347976Abstract: Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses a solid-phase extraction material that includes a hydrophilic solid support at least partially embedded within a hydrophobic matrix.Type: GrantFiled: December 20, 2001Date of Patent: March 25, 2008Assignee: 3M Innovative Properties CompanyInventors: Ranjani V. Parthasarathy, Raj Rajagopal, Vicky L. Morris, William Bedingham, Barry W. Robole
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Patent number: 7348157Abstract: A method for detection and/or quantification of cardiac troponin I (cTnI) in a sample derived from an individual's blood, the method being based on a sandwich immunoassay employing at least two capture antibodies and at least one tracer antibody, in which a first capture antibody is directed to the N-terminal part of cTnI, to the C-terminal part of cTnI or to the part of the cTnI midfragment, which is slightly affected by the interfering factor, and a second capture antibody is directed to another of these parts, and a tracer antibody is directed to the N-terminal part of cTnI, to the C-terminal part of cTnI or to TnC, which is complexed with cTnI.Type: GrantFiled: April 7, 2004Date of Patent: March 25, 2008Assignee: University of TurkuInventors: Susann Eriksson, Kim Pettersson
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Patent number: 7344890Abstract: A method for discriminating and quantifying platelets within an analyzed blood sample involves initially diluting the blood sample with a ghosting reagent that causes a change in the index of refraction of the red blood cells. Owing to the change in the index of refraction, light scattered from the ghosted red blood cells will be substantially reduced relative to light scattered from platelets. This results in locations of platelets within a scatterplot of the analyzed blood sample to fall within a region distinguishable from those containing normal red blood cells, fragmented red blood cells, and microcytic red blood cells.Type: GrantFiled: November 9, 2005Date of Patent: March 18, 2008Assignee: Beckman Coulter, Inc.Inventors: Carlos A. Perez, Lidice L. Lopez, Mark A. Wells, Joaquin Ibanez, Eileen Landrum, Roberto Del Valle, Santiago Galvez
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Patent number: 7332346Abstract: The method is intended to detect with high sensitivity and high accuracy chemically contaminating impurities in which there exist chemically contaminating impurity constituents of basic series, acidic series, and organic substance series, in gaseous and particle states, and constituents of compounds thereof, contained in air inside a clean room, as constituents having every property. To this end, ions composed of various cations and anions, constituting the chemically contaminating impurities in fine particle, gaseous, or molecular state, are caused to continuously undergo dissolution reaction and dissociation reaction in a solution in sequence from those constituting salts having strong solubility or strong dissociative tendency.Type: GrantFiled: October 31, 2002Date of Patent: February 19, 2008Assignee: Oki Electric Industry Co., Ltd.Inventor: Hidetoshi Wakamatsu
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Patent number: 7326386Abstract: The apparatus for extracting nucleic acid includes a cartridge holder 61 which holds nucleic acid extraction cartridges 11, a container holder 62 which holds waste liquid containers 12 and recovering containers 13 and a container holding table 63 which holds the cartridge holder 61 and the container holder 62, the container holding table 63 is attachable to and detachable from an apparatus main body 2 with the cartridge holder 61 holding the nucleic acid extraction cartridges and the container holder 62 holding the waste liquid containers and the recovering containers held by the container holding table 63.Type: GrantFiled: January 27, 2006Date of Patent: February 5, 2008Assignee: Fujifilm CorporationInventors: Hidemi Sasaki, Katsuya Inana
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Patent number: 7326577Abstract: A new method of cell fixation using a mild denaturing heat and the fixative PERMIFLOW™ or similar fixative that preserves cell morphology, light scatter profiles, nucleic acid content, cell surface epitopes and unmasks internal epitopes previously not available. The method can be combined with analysis of each of these parameters by flow cytometry and thus has application in drug screening and patient or tumor monitoring protocols.Type: GrantFiled: August 13, 2004Date of Patent: February 5, 2008Assignee: Esoterix, Inc.Inventors: Keith E. Shults, Angel L. Flye
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Patent number: 7323342Abstract: A method for determination for a given oil the relative stability of a water-in-oil emulsion that will be formed by that oil with water comprises measuring for the given oil the weight fraction of the oil that is most strongly adsorbed on a silica gel column successively eluted with n-hexane, toluene and methylene chloride-methanol mixture solvents and determining whether said weight fraction is greater than about 0.05; with a value above 0.05 being determinative of an emulsion more stable than one with a value less than 0.05.Type: GrantFiled: January 30, 2004Date of Patent: January 29, 2008Assignee: ExxonMobil Research and Engineering CompanyInventor: Ramesh Varadaraj
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Patent number: 7323144Abstract: A simplified collecting operation of a biological sample is provided, which enables reduction of the examination cost.Type: GrantFiled: March 7, 2003Date of Patent: January 29, 2008Assignee: Leisure, Inc.Inventors: Kousuke Arai, Noriyuki Nomura
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Patent number: 7323344Abstract: A method is provided for the quantitative analysis of the contents, in nitrogen, of hydrogen and methane by ionic mobility spectrometry. The method includes the steps of: a) performing a measurement of the apparent hydrogen concentration in the nitrogen to be analyzed; b) performing a measurement of the apparent hydrogen concentration in a flow of the same sample of nitrogen, purified of all impurities but methane; and c) comparing the two measurements. A system of branched lines is also provided for carrying out the method.Type: GrantFiled: November 6, 2003Date of Patent: January 29, 2008Assignee: Saes Getters S.p.A.Inventors: Luca Pusterla, Marco Succi
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Patent number: 7309608Abstract: A method of identification and quantitative analysis of aldehydes and/or ketones in a sample by mass spectrometry using stable isotope labeled oxime internal standards or stable isotope labeled hydrazone internal standards is provided. Stable isotope labeled oxime internal standards are synthesized by reaction of an authentic sample of aldehydes and/or ketones with a stable isotope labeled alkoxylamine reagent while stable isotope labeled hydrazone internal standards are synthesized by reaction of an authentic sample of aldehydes and/or ketones with a stable isotope labeled alkylhydrazine reagent. A non labeled version of the stable isotope labeled reagent is used to convert aldehydes and/or ketones in the sample to the non labeled version of the stable isotope labeled oxime or hydrazone internal standards.Type: GrantFiled: September 30, 2003Date of Patent: December 18, 2007Inventors: Hoa Duc Nguyen, Trinh Duc Nguyen, Duc Tien Nguyen
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Publication number: 20070275478Abstract: A system and corresponding method for processing of biological samples prior to spectroscopy analysis. The system includes a support for an organic sample, a solution applicator configured to apply a solution for extraction of at least one biomarker protein from the organic sample. The system includes a digester-medium applicator configured to apply to the organic sample a digesting medium capable of at least partial digestion of the biomarker proteins into peptides. The system includes a heating device configured to heat at least one of the organic sample, the solution, the digesting medium, and the biomarker proteins to a temperature above room temperature.Type: ApplicationFiled: May 26, 2006Publication date: November 29, 2007Applicant: Science & Engineering Services, Inc.Inventors: Nelli I. Taranenko, Berk Oktem, Appavu K. Sundaram, Vladimir M. Doroshenko
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Patent number: 7297549Abstract: A method of determining bias in a measurement of a constituent concentration level in a sample gas is provided. The method comprises establishing a sample gas flow from an emission stream into a sample gas line of an emissions monitoring system. The method further comprises removing water from the sample gas flow and cooling the sample gas flow to a temperature below about 41° F. to produce a cooled, dried sample gas flow. The constituent concentration level is then determined for the cooled, dried sample gas flow. The method further comprises introducing a span gas having a known span gas constituent concentration level into the sample gas flow to form a combined sample and span gas flow, the span gas being introduced at a desired span gas flow rate. The method still further comprises removing water from the combined sample and span gas and cooling the combined sample and span gas to a temperature below about 41° F. to produce a cooled, dried, combined sample and span gas flow.Type: GrantFiled: March 6, 2002Date of Patent: November 20, 2007Assignee: General Electric CompanyInventors: William Steven Lanier, Glenn England
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Patent number: 7297551Abstract: Recombinant fragments of Factor C are disclosed. These proteins and peptides show great potency in recognizing, binding to, neutralizing and removing endotoxin. These molecules can thus be used for anti-microbial, anti-endotoxin, and anti-sepsis therapy. SSCrFCES is a 38 kDa protein representing the LPS-binding domain of Factor C. The ability of SSCrFCES to bind lipid A was analyzed using an ELISA-based assay as well as surface plasmon resonance. Surface plasmon resonance similarly carried out for SSCrFC-sushi-1,2,3-GFP, SSCrFC-sushi-1GFP, and SSCrFC-sushi-3GFP confirmed their superior affinity for endotoxin. The 50% endotoxin-neutralizing concentration of SSCrFCES against 200 EU of endotoxin is 0.069 ?M, suggesting that SSCrFCES is an effective inhibitor of LAL coagulation cascade. Although partially attenuated by human serum, as low as 1 ?M of SSCrFCES inhibits the LPS-induced secretion of hTNF-? and hIL-8 by THP-1 and human pheripheral blood mononuclear cells with a potency more superior than polymyxin B.Type: GrantFiled: August 7, 2003Date of Patent: November 20, 2007Assignee: National University of SingaporeInventors: Jeak L. Ding, BoW Ho, Nguan S. Tan
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Patent number: 7276382Abstract: A blood test method including (a) centrifuging a mammalian blood sample into a plasma and blood cells; (b) removing buffy coats from the blood cells to obtain red blood cells containing solutes confined therewithin; (c) washing the red blood cells with a buffered physiological saline solution and isolating the washed blood cells; (d) mixing the washed red blood cells with a buffered physiological saline solution to obtain a suspended liquid; (e) centrifuging the suspended liquid to remove a supernatant and to obtain a red blood layer; (f) mixing the red blood layer with a hypertonic solution and maintaining the resulting suspension at a temperature of 25 to 40° C.Type: GrantFiled: October 2, 2003Date of Patent: October 2, 2007Inventors: Noboru Horiguchi, Hiroshi Horiguchi
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Patent number: 7259020Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.Type: GrantFiled: July 30, 2002Date of Patent: August 21, 2007Assignee: Applera CorporationInventors: Steven A. Sundberg, J. Wallace Parce, Calvin Y. H. Chow
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Patent number: 7244395Abstract: A method and system for performing low level sulfur UV fluorescence measures including an UV interference reduction system which removes or destroys interfering nitrogen oxides. The preferred nitrogen removal systems include introducing ozone into the system in sufficient quantities to destroy any produce NO and optionally a nitrogen sparge or similar nitrogen gas removal system.Type: GrantFiled: September 29, 2003Date of Patent: July 17, 2007Assignee: Petroleum Analyzer Company, LPInventor: Franek Olstowski
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Patent number: 7238532Abstract: A method for measuring the concentration of isothiazolones in aqueous systems including removing sample interferences by lowering the pH of a sample collected from the aqueous system containing isothiazolones and filtering the sample, removing additional interferences by raising the pH and subsequent filtering, selectively adsorbing the isothiazolones in the sample desorbing the isothiazolones from the adsorbent, and comparing the absorbance of ultra-violet light of the desorbed sample to a standard of known concentration.Type: GrantFiled: June 3, 2002Date of Patent: July 3, 2007Inventor: David Michael Philbrook
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Patent number: 7226562Abstract: The present invention provides an apparatus and method for storing a particle-containing liquid. The storage apparatus comprises a microfluidic convoluted flow channel having a plurality of particle capture regions. The storage channel is preferably an isotropic spatially periodic channel. Sedimented particles can be resuspended following storage. This invention further provides a microfluidic analysis cartridge having a convoluted storage channel therein. The sample analysis can use optical, electrical, pressure sensitive, or flow sensitive detection. A plurality of analysis channels can be included in a single cartridge. The analysis channels can be joined to reagent inlets for diluents, indicators or lysing agents. A mixing channel can be positioned between the reagent inlet and the analysis region to allow mixing and reaction of the reagent. The cartridge can include additional valves and pumps for flow management.Type: GrantFiled: August 2, 2005Date of Patent: June 5, 2007Assignee: University of WashingtonInventors: Mark R Holl, Floyd Edwards, Robert J Morff, Gerald L Klein
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Patent number: 7226793Abstract: A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.Type: GrantFiled: June 12, 2003Date of Patent: June 5, 2007Assignee: Quidel CorporationInventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer
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Patent number: 7214348Abstract: Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.Type: GrantFiled: January 3, 2003Date of Patent: May 8, 2007Assignee: Applera CorporationInventors: Sean M. Desmond, Zbigniew T. Bryning, John Shigeura, Gary Lim, Adrian Fawcett, Jacob K. Freudenthal, Gary Bordenkircher
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Patent number: 7211376Abstract: The invention provides methods, compositions, apparatus, and a computer data retrieval system for conducting proteomics.Type: GrantFiled: November 21, 2003Date of Patent: May 1, 2007Assignee: Target Discovery, Inc.Inventors: Luke V. Schneider, Michael P. Hall, Robert Petesch, Jeffrey N. Peterson
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Patent number: 7208319Abstract: Methods are provided for measurement of nucleated red blood cells in a blood sample. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, DC impedance, and medium angle light scatter measurements; by axial light loss, low angle light scatter, and medium angle light scatter measurements; or by axial light loss, DC impedance, low angle light scatter, and medium angle light scatter measurements; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.Type: GrantFiled: January 24, 2006Date of Patent: April 24, 2007Assignee: Beckman Coulter, Inc.Inventors: Lidice L. Lopez, Carlos A. Perez, Mark A. Wells, Diana B. Careaga, Ziling Huo, Cheng Qian, Nancy J. Torke
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Patent number: 7205152Abstract: A self-contained closed loop system and method for detecting contaminants in, on, and around objects. The system includes an air duct subsystem connecting at least one sensor to a sealed housing containing a rotating container. Air from the sealed housing is circulated past a sensor to detect, for example, biological or chemical contaminants. If a contaminant is detected, an indicator is set and a contaminant neutralizer is optionally injected into the air duct subsystem.Type: GrantFiled: July 22, 2002Date of Patent: April 17, 2007Assignee: Lockheed Martin CorporationInventor: John T. Swider
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Patent number: 7198955Abstract: A method is disclosed whereby the concentration of a blood substitute, such as cross-linked hemoglobin, in a serum or plasma specimen is rapidly and accurately identified and quantified. The method further takes the measured concentration of the blood substitute and uses it to correct for its effect, if any, on a measured analyte concentration, e.g., serum/plasma total protein. Further, the method allows for the determination of the concentration of true hemoglobin in the presence of blood substitutes. The method is carried out in respect of samples contained in a primary or secondary labelled tube, or a pipette tip used to dispense serum or plasma in a blood analyzer.Type: GrantFiled: October 16, 1997Date of Patent: April 3, 2007Assignee: NIR Diagnostics Inc.Inventors: James Samsoondar, Duncan MacIntyre
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Patent number: 7195923Abstract: The present invention provides methods for determining a ratio of an amount of a glycated form of a protein to a total amount of the protein in a sample containing the glycated protein, the glycosylated protein, or the glycoprotein. The method incorporates lateral flow test strip or vertical flow test strip devices having negatively charged carboxyl or carboxylate groups and hydroxyboryl groups immobilized and interspersed on a solid support matrix. The solid support matrix may include derivatives of cellulose (e.g., carboxy cellulose) derivatized with carboxylic acid (e.g., carboxylate, carboxyl) groups and hydroxyboryl compounds including phenylboronic acid (e.g., phenylborate), aminophenylboronic acid, boric acid (e.g., borate), or other boronic acid (e.g., boronate) compounds. The present invention is usefi.il for monitoring glycation or glycosylation of hemoglobin or albumin for monitoring glycemic control (e.g., glycemia in diabetes).Type: GrantFiled: January 31, 2002Date of Patent: March 27, 2007Assignee: Scripps Laboratories, Inc.Inventors: Ralph P. McCroskey, Cameron E. Melton
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Patent number: 7192560Abstract: Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material.Type: GrantFiled: December 20, 2001Date of Patent: March 20, 2007Assignee: 3M Innovative Properties CompanyInventors: Ranjani V. Parthasarathy, Raj Rajagopal, Erin E. Olson, Frank J. Beissel, IV, William Bedingham, Barry W. Robole
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Patent number: 7192697Abstract: A modified limulus test procedure is provided to measure the lipopolysaccharide (LPS), lipid A, and/or glucan reactivity in a biological liquid. The test procedure is independent of the anti-limulus factors present in the sample. The procedure involves strong oxidative treatment of the biological liquid sample.Type: GrantFiled: May 25, 2005Date of Patent: March 20, 2007Inventor: Thomas W. Stief
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Patent number: 7189579Abstract: A method and device for measuring release rates of contaminants in at least one of a fast release mode and a slow release mode in which a volatile liquid sample is introduced into a sealed transparent reactor vessel having at least one sorbent contained within the transparent reactor vessel and a separator for preventing direct contact between the at least one adsorbent and the at least one volatile liquid sample in the transparent reactor vessel, whereby substantially zero headspace is maintained within the transparent reactor vessel. At least one solvent soluble constituent present in the at least one volatile liquid sample is passed through the separator, resulting in sorption of the at least one solvent soluble constituent by the at least one sorbent. In accordance with one preferred embodiment, the separator is a dialysis bag contained in the transparent reactor vessel into which the resin is placed.Type: GrantFiled: February 22, 2002Date of Patent: March 13, 2007Assignee: Gas Technology InstituteInventors: Bhupendra K. Soni, Thomas D. Hayes
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Patent number: 7189373Abstract: Carbon monoxide contained in reformate gas is removed by a preferential oxidation reaction in a catalyst, two preferential oxidation reactors (20A, 20B) being disposed in series. Valves (7, 8) supply air containing oxygen as an oxidizing agent individually to these preferential oxidation reactors (20A, 20B). Temperature sensors (9, 10) detect the catalyst temperatures of the preferential oxidation reactors (20A, 20B), and a controller (5), by adjusting the flow rate of the valves (7, 8) based on the detected temperatures, maximizes the carbon monoxide removal performance of the preferential oxidation reactors (20A, 20B), while preventing excessive catalyst temperature rise.Type: GrantFiled: February 19, 2003Date of Patent: March 13, 2007Assignee: Nissan Motor Co., Ltd.Inventors: Ikuhiro Taniguchi, Hiroaki Hashigaya
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Patent number: 7186379Abstract: This invention provides a method and system capable of the continuous, real-time measurement of low concentrations of aqueous free cyanide (CN) using an on-line, flow through system. The system is based on the selective reactivity of cyanide anions and the characteristically nonreactive nature of metallic gold films, wherein this selective reactivity is exploited as an indirect measurement for aqueous cyanide. In the present invention the dissolution of gold, due to the solubilization reaction with the analyte cyanide anion, is monitored using a piezoelectric microbalance contained within a flow cell.Type: GrantFiled: February 7, 2002Date of Patent: March 6, 2007Assignee: Battelle Energy Alliance, LLCInventors: Jeffrey J. Rosentreter, Kevin L. Gering
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Patent number: 7183118Abstract: The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.Type: GrantFiled: June 3, 2003Date of Patent: February 27, 2007Assignee: The Institute for Systems BiologyInventors: Rudolf H. Aebersold, Hui Zhang
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Patent number: 7179657Abstract: A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.Type: GrantFiled: September 10, 2002Date of Patent: February 20, 2007Assignee: Quidel CorporationInventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer, Paul Lambotte, Paul J. Lawrence
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Patent number: 7179655Abstract: Methods and compositions are described for analyzing complex protein mixtures, such as proteomes, using activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Labeled peptides obtained from the labeled active target proteins can be used in screening and identification procedures, and can be related to the identity, presence, amount, or activity of active members of the desired target protein class. The methods and compositions described herein can be used, for example, to provide diagnostic information concerning pathogenic states, in identifying proteins that may act as therapeutic targets, and in drug discovery.Type: GrantFiled: March 1, 2002Date of Patent: February 20, 2007Assignee: Activx Biosciences, Inc.Inventor: Matthew Patricelli
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Patent number: 7166477Abstract: A method of preparing a sample of muscle tissue and of assaying the prepared sample to determine the presence of prions in the sample is disclosed. The muscle tissue is homogenized and mixed with a complexing agent which forms a complex with a higher specific gravity than PrPSc, the complexing agent or other components of the homogenate. Gravity is then used (e.g. ultra centrifugation) to concentrate the complex and the concentrate is assayed to detect prions. The muscle tissue is preferably extracted from a muscle or group of muscles such as hind limb muscle which have a higher or more preferably the highest concentration of prions as compared to other muscle in the mammal.Type: GrantFiled: August 2, 2002Date of Patent: January 23, 2007Assignee: The Regents of the University of CaliforniaInventors: Stanley B. Prusiner, Patrick Bosque
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Patent number: 7163660Abstract: Apparatus for taking up liquid analytes having a microtitre plate with a plurality of wells and a plurality of pipettes, as well as a pump, which is coupled to several pipettes in such a way that an analytes can be simultaneously sucked out of several wells or introduced into several wells by actuating the pump.Type: GrantFiled: May 30, 2001Date of Patent: January 16, 2007Assignee: Infineon Technologies AGInventor: Volker Lehmann
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Patent number: 7135341Abstract: A method of making a reference control containing a nucleated red blood cell component includes providing a blood cell containing a nucleus; treating the blood cell with a treatment solution to alter a nucleus property from a natural value to a target value suitable for simulating nucleated red blood cells on a blood analyzer; and suspending treated blood cell in a suspension medium to form a reference control. The method also includes integrating the nucleated red blood cell component with white blood cell, red blood cell, platelet and reticulocyte components. Further disclosed is a cell treatment composition for altering a nucleus property, which includes a conditioning component, a lytic component for permeating cell membrane, and a fixing component for preserving the cell nucleus. Also disclosed is a method of using the reference control for measurement of nucleated red blood cells on a blood analyzer.Type: GrantFiled: March 30, 2005Date of Patent: November 14, 2006Assignee: Beckman Coulter, Inc.Inventors: Nery Ortiz, Sandra Socarras
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Patent number: 7129093Abstract: A method of determining beryllium or a beryllium compound thereof in a sample, includes providing a sample suspected of comprising beryllium or a compound thereof, extracting beryllium or a compound thereof from the sample by dissolving in a solution, adding a fluorescent indicator to the solution to thereby bind any beryllium or a compound thereof to the fluorescent indicator, and determining the presence or amount of any beryllium or a compound thereof in the sample by measuring fluorescence.Type: GrantFiled: March 30, 2004Date of Patent: October 31, 2006Assignee: Los Alamos National Security, LLCInventors: T. Mark McCleskey, Deborah S. Ehler, Kevin D. John, Anthony K. Burrell, Gavin E. Collis, Edel M. Minogue, Benjamin P. Warner
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Patent number: 7125696Abstract: A invention concerns a novel method for preparing a polypeptide soluble in an aqueous solvent in the absence of detergent, and polypeptides obtainable by said method. The invention also concerns the use of said polypeptides, in particular for preparing medicines or vaccines, for fighting against bacterial and viral infections or cancers.Type: GrantFiled: January 4, 2001Date of Patent: October 24, 2006Assignee: Pierre Fabre MedicamentInventors: Thierry Baussant, Pascale Jeannin, Yves Delneste, François Lawny, Jean-Yves Bonnefoy