Dilution Patents (Class 436/179)
  • Patent number: 10101346
    Abstract: In accordance with embodiments, a transport mechanism transports a first sample container to a sample aspiration position. A setting part may set a second sample container, that accommodates a sample to be measured, with priority over a measurement of a sample in a first sample container. A nozzle may be capable of moving a sample from a first sample container at a sample aspiration position, and aspirating the sample from the second sample container. A detector may detect components of the sample. A controller may control a transport mechanism such that the first sample container moves to a position distant from the sample aspiration position, when the first sample container has been transported and when sample aspiration of the second sample container is required. A controller may execute control to move the nozzle above the sample aspiration position in state in which the first sample container is distant from the sample aspiration position.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: October 16, 2018
    Assignee: SYSMEX CORPORATION
    Inventors: Hiroki Koike, Yousuke Matsui, Go Senda, Hiroo Tatsutani, Yuichiro Ohmae, Atsushi Kumagai, Tomoyuki Asahara
  • Patent number: 9841406
    Abstract: A switching valve includes: (A) a rotor including: (1) a center pipe connection port, (2) a first in-valve flow path in communication with the center pipe connection port, and (3) an arc-like second in-valve flow path; (B) a stator including: (4) a first pipe connection port group which is brought into communication independently with the center pipe connection port via the first in-valve flow path when the rotor is turned, and (5) a second pipe connection port group which is brought into mutual communication via the second in-valve flow path when the rotor is turned; and (C) an arrangement of the rotor and the stator satisfying the following relationship: the state of communication or non-communication among the second pipe connection port group via the second in-valve flow path is switched in accordance with the state of communication between the first pipe connection port group and the center pipe connection port.
    Type: Grant
    Filed: April 22, 2014
    Date of Patent: December 12, 2017
    Assignee: Sekisui Medical Co., Ltd.
    Inventors: Takuya Yotani, Hiroaki Taira, Takayuki Oka, Hideki Muraki
  • Patent number: 9359649
    Abstract: The present invention provides methods for collecting, storing or transporting liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released for subsequent analysis by compressing or centrifuging the matrix. Also provided are method of using kits for collecting, storing, transporting and recovering biological specimens containing analytes of interest.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: June 7, 2016
    Assignee: VIVEBIO, LLC
    Inventors: Robert M. Lloyd, Jr., Darrell A. Burns, Joe T. Huong
  • Patent number: 9050595
    Abstract: The invention is to devices and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature for forming a diluted sample for analysis. The devices and methods also include a dilution verification feature for verifying the degree of dilution of the diluted sample. The devices preferably are capable of being used in the point-of-care diagnostic field is provided.
    Type: Grant
    Filed: December 1, 2011
    Date of Patent: June 9, 2015
    Assignee: Abbott Point of Care Inc.
    Inventors: Cary James Miller, Graham Davis, Michael Zelin
  • Patent number: 9029165
    Abstract: A method for detecting electromagnetic waves derived from bacterial DNA, comprising extracting and purifying nucleic acids from a sample; diluting the extracted purified nucleic acids in an aqueous solvent; measuring a low frequency electromagnetic emission over time from the diluted extracted purified nucleic acids in an aqueous solvent; performing a signal analysis of the low frequency electromagnetic emission over time; and producing an output, based on the signal analysis, in dependence on the DNA in the sample. The DNA may be extracted from at least one of blood, feces, urine, saliva, tears, seminal fluid, sweat, seminal and vaginal fluids of a patient, or water to determine, e.g., potability. The samples may be frozen. The extracting and purifying may comprise diluting the sample with an aqueous buffer and mixing; degrading proteins in the diluted sample; precipitating DNA from the buffer solution; and resuspending the precipitated DNA in an aqueous solution.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: May 12, 2015
    Inventor: Luc Montagnier
  • Patent number: 8991239
    Abstract: A system and method for preparing samples for analyte testing. The sample preparation system can include a freestanding receptacle. The method can include providing a liquid composition comprising a source and a diluent, and positioning the liquid composition in a reservoir defined by the freestanding receptacle. The method can further include filtering the liquid composition to form a filtrate comprising an analyte of interest, removing at least a portion of the filtrate from the sample preparation system to form a sample, and analyzing the sample for the analyte of interest.
    Type: Grant
    Filed: August 10, 2012
    Date of Patent: March 31, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Kurt J. Halverson, Stephen C. P. Joseph, Matthew T. Scholz
  • Patent number: 8951805
    Abstract: A system for managing bulk liquids for an automated clinical analyzer. The system comprises (a) at least one local reservoir for storing a bulk liquid for impending use, (b) at least one container for holding a bulk liquid before the liquid is transferred to a local reservoir, and (c) a controller for monitoring the level of a bulk liquid in a local reservoir. The local reservoir for storing a bulk liquid for impending use can be a trough. The use of troughs for storing a reagent, a diluent, or some other treating agent for impending use enables an aspirating/dispensing device having a plurality of pipettes to aspirate and dispense the reagent, diluent, or other treating agent at a high rate of throughput. The controller can monitor the level of a liquid in (a) a local reservoir for storing a bulk liquid for imminent use and the level of liquid in a (b) container for holding a bulk liquid before the liquid is transferred to a local reservoir.
    Type: Grant
    Filed: April 8, 2014
    Date of Patent: February 10, 2015
    Assignee: Abbott Laboratories
    Inventors: Patrick P. Fritchie, Gregory E. Gardner, Richard W. Mahoney
  • Patent number: 8932863
    Abstract: Methods for evaluating a fuel are provided. In one embodiment, a method of evaluating a fuel includes providing a testing specimen of the fuel. Also, the method includes analyzing the testing specimen and identifying a compound in the testing specimen. The method also provides for determining the fuel is biologically-sourced based on the identified trace compound.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: January 13, 2015
    Assignee: UOP LLC
    Inventors: Haiyan Wang, Michael J. McCall
  • Patent number: 8906302
    Abstract: A reagent preparing device for preparing a reagent to be supplied to a measurement device for measuring a specimen, comprising: a first liquid storage unit for storing a first liquid; a reagent storage unit for storing a prepared reagent, including the first liquid and a second liquid different from the first liquid; a first liquid discarding unit for discarding the first liquid stored in the first liquid storage unit; and a controller configured for measuring an accumulated time of the first liquid in the first liquid storage unit; and controlling the first liquid discarding unit to discard the first liquid stored in the first liquid storage unit when the accumulated time reaches a predetermined time is disclosed. A specimen measuring device and a reagent preparing method are also disclosed.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: December 9, 2014
    Assignee: Sysmex Corporation
    Inventors: Noriyuki Nakanishi, Koichi Okubo, Masahiko Oguro, Tomoyuki Asahara
  • Patent number: 8894932
    Abstract: A reagent preparing device capable of supplying a predetermined reagent, which includes a first liquid and a second liquid different from the first liquid, to a measurement section for measuring a specimen using the reagent, comprising: a reagent preparing section for preparing the predetermined reagent; a characteristic measurement device for measuring characteristic of the reagent prepared by the reagent preparing section; and a controller configured for performing operations comprising, controlling the supply of reagent prepared by the reagent preparing section to the measurement section according to the measurement result by the characteristic measurement device, and calibrating the characteristic measurement device based on a known characteristic value of a standard liquid having the known characteristic value and a measurement result obtained by measuring the characteristic of the standard liquid by the characteristic measurement device, is disclosed. A specimen processing system is also disclosed.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: November 25, 2014
    Assignee: Sysmex Corporation
    Inventors: Koichi Okubo, Noriyuki Nakanishi, Masahiko Oguro, Tomoyuki Asahara, Takayuki Nakajima
  • Patent number: 8846410
    Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
    Type: Grant
    Filed: November 15, 2013
    Date of Patent: September 30, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Ramakrishna Sista, Arjun Sudarsan
  • Patent number: 8828335
    Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: September 9, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Manu Prakash, Neil Gershenfeld
  • Patent number: 8809068
    Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field wherein droplet operations do not allow magnetically responsive beads to be introduced into a region of the magnetic field which is sufficiently non-uniform to cause bead clumping resulting in a more homogenous distribution of the beads in the droplet. Other embodiments are also provided.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: August 19, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Allen Eckhardt
  • Patent number: 8758703
    Abstract: The present invention provides a pump device 50 which is usable to dilute a sample 52 before analysis. A first pump 54 pumps the sample to a mixing region 58 where it mixes with a diluent 66. A second pump 64 pumps the diluted sample to the analysis instrument. The flow of the diluent to the mixer is equal to the difference of the flow of the sample to the mixer and the flow of the diluted sample to the instrument. Pumps 54 and 64 are independently controllable by a controller unit which is arranged so that data from the instrument can be used to determine the dilution factor of the sample. Thus, the controller can control this dilution factor in real time, upon receipt of such data from the instrument, by change either one of (or both) the pump's flow rate.
    Type: Grant
    Filed: May 19, 2011
    Date of Patent: June 24, 2014
    Assignee: Thermo Fisher Scientific Inc.
    Inventors: Philip Neil Shaw, Philip Marriott
  • Patent number: 8748191
    Abstract: Analytical systems and methods are provided for simultaneously dispensing metered volumes of fluids at different rates and mixing the fluids to generate a mixed sample having the fluids in proportion to the different rates at which they were dispensed. In some cases two or more of the fluids are premixed prior to mixing with other fluids. In some cases a use composition and diluent are simultaneously dispensed at different rates and premixed to form a diluted sample. One or more reagents may be mixed with the diluted sample and the sample mixture can be analyzed to determine characteristics of the use composition.
    Type: Grant
    Filed: August 2, 2010
    Date of Patent: June 10, 2014
    Assignee: Ecolab USA Inc.
    Inventors: Paul R. Kraus, William M. Christensen
  • Patent number: 8741660
    Abstract: The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: June 3, 2014
    Assignee: Stokes Bio Limited
    Inventors: Mark Davies, Tara Dalton, Michael Sayers, Brian Chawke
  • Patent number: 8685748
    Abstract: The present invention provides methods for collecting, storing or transporting liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released for subsequent analysis by compressing or centrifuging the matrix. Also provided are method of using kits for collecting, storing, transporting and recovering biological specimens containing analytes of interest.
    Type: Grant
    Filed: June 11, 2009
    Date of Patent: April 1, 2014
    Assignee: Vivebio, LLC
    Inventors: Robert M. Lloyd, Jr., Darrell A. Burns, Joe T. Huong
  • Patent number: 8647879
    Abstract: A peptide probe that generates fluorescence signals rapidly upon recognition of various A? aggregates without significant perturbation of samples. The present peptide probes display an increase in fluorescence signals upon coincubation with A? oligomers, but neither monomeric/dimeric species nor fibrils. The detection can occur within an hour or two without any additional sample preparation and incubation steps.
    Type: Grant
    Filed: August 13, 2010
    Date of Patent: February 11, 2014
    Assignee: Polytechnic Institute of NYU
    Inventors: Jin Ryoun Kim, Yang Hu, Jorge Ghiso
  • Patent number: 8637324
    Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
    Type: Grant
    Filed: April 7, 2011
    Date of Patent: January 28, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Ramakrishna Sista, Arjun Sudarsan
  • Patent number: 8637317
    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte.
    Type: Grant
    Filed: January 6, 2011
    Date of Patent: January 28, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Vamsee K. Pamula, Arjun Sudarsan, Ramakrishna Sista
  • Patent number: 8623665
    Abstract: A method for collecting and processing biological samples (e.g., fecal samples) is disclosed. The method may include obtaining a system comprising a container, a collector, and a lid. Using the collector, a user may collect a sample. The sample may be inserted within the container. The lid may be secured and the container and sample contained therewithin may be transported to a testing facility. At the testing facility, the container may be used throughout the processing of the sample. Accordingly, the risk of cross-contamination may be reduced.
    Type: Grant
    Filed: October 13, 2011
    Date of Patent: January 7, 2014
    Inventors: Val L. Poll, Steven J. Miller, Jay J. Davis, Brian T. Leishman, Joshua L. Kirk
  • Publication number: 20140004614
    Abstract: A method of changing the response level for any chemical test kits designed for the determination of a metal or compound (such as lead) in paint and other media is disclosed. The invention solves two common problems that exist with using chemical test kits for the detection of lead in paint or other coatings and media: (1) conversion of the paint sample into particles small enough to dissolve the metal in the sample; and, (2) controlling the amount of (paint) sample that gets exposed to the chemicals. This is accomplished by using a coffee grinder or equivalent tool plus a solid (food) product to break up the paint sample into small particles and at the same time dilute the concentration of the metal, while in solid form, down to a level that results in obtaining a positive response at the desired concentration.
    Type: Application
    Filed: June 30, 2012
    Publication date: January 2, 2014
    Applicant: QUANTECH, INC.
    Inventors: Frederic G. Dewalt, David C. Cox
  • Patent number: 8617901
    Abstract: A comprehensive approach for interpretation of the multiple spiking isotope dilution results is described herein. It has now been found that a method of multiple spiking isotope dilution analysis for mass spectrometry is possible using an approach that permits precise and simultaneous characterization of m substances from a sample even if species inter-conversion (degradation and formation) has occurred prior to separation. Advantageously, initial and final amounts of involved analytes, conversion extent, conversion degree and rate constants from the results of a single quantitation experiment may be obtained with the present method. In a particularly advantageous embodiment, uncertainty in the characterization of the substances may be estimated more accurately by also estimating increase in the uncertainty due to inter-conversion of the analytes.
    Type: Grant
    Filed: November 18, 2009
    Date of Patent: December 31, 2013
    Inventors: Laurent Ouerdane, Juris Meija, Zoltan Mester
  • Patent number: 8613889
    Abstract: The present invention relates to droplet-based washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
    Type: Grant
    Filed: December 15, 2006
    Date of Patent: December 24, 2013
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Vijay Srinivasan, Richard B. Fair
  • Patent number: 8592219
    Abstract: A method for transporting in a microfluidic conduit an aliquot of a liquid, possibly containing a substance I, which is dissolved in the liquid and typically exhibits charged groups and/or hydrophobic groups. The method is characterized in that the liquid contains an amphiphilic macromolecular substance.
    Type: Grant
    Filed: January 20, 2005
    Date of Patent: November 26, 2013
    Assignee: Gyros Patent AB
    Inventors: Rickard Kange, Mats Inganas, Magnus Gustafsson, Johan Engstrom, Bo Ek, Helene Dérand, Ann-Kristine Honerud
  • Patent number: 8580575
    Abstract: Disclosed herein is a reactor plate which prevents the entry of foreign matter from outside and the pollution of an outside environment. The reactor plate includes a sealed reaction well (5), reaction well channels (13, 15, 17) connected to the reaction well (5), and a syringe (51) for sending a liquid to the reaction well channels (13, 15, 17) and the reaction well (5). The syringe (51) has a cylinder (51a), a plunger (51b), and a cover body (51d). The cover body (51d) has flexibility in the sliding direction of the plunger (51b), and is connected to the cylinder (51a) and the plunger (51b) to create a sealed space (51e) enclosed with the cylinder (51a), the plunger (51b), and the cover body (51d). The cover body (51d) is provided to hermetically cut off a part of an inner wall of the cylinder (51a) to be brought into contact with the plunger (51b) from an atmosphere outside the cylinder (51a).
    Type: Grant
    Filed: February 29, 2008
    Date of Patent: November 12, 2013
    Assignee: Shimadzu Corporation
    Inventor: Nobuhiro Hanafusa
  • Patent number: 8563323
    Abstract: The present invention demonstrates that the diagnosis and prediction of clinical behavior in patients with hematologic malignancies, such as leukemia, can be accomplished by analysis of proteins present in a plasma sample. Thus, in particular embodiments the present invention uses plasma to create a diagnostic or prognostic protein profile of a hematologic malignancy comprising collecting plasma samples from a population of patients with hematologic malignancies; generating protein spectra from the plasma samples with or without fractionation; comparing the protein spectra with clinical data; and identifying protein markers in the plasma samples that correlate with the clinical data. Protein markers identified by this approach can then be used to create a protein profile that can be used to diagnose the hematologic malignancy or determine the prognosis of the hematologic malignancy. Potentially these specific proteins can be identified and targeted in the therapy of these malignancies.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: October 22, 2013
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Elihu H. Estey, Hagop M. Kantarjian, Francis J. Giles, Michael J. Keating
  • Patent number: 8496878
    Abstract: An immunodiagnostic test card includes a flat planar member and at least one dilution chamber that is supported by the flat planar member. The at least one dilution chamber can be disposed adjacent chambers used for testing a patient sample that are provided on the immunodiagnostic test card or can be provided separately.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: July 30, 2013
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Raymond Francis Jakubowicz, William G. Atterbury, Douglas E. Boyd, Michael L. Marshall
  • Patent number: 8476080
    Abstract: A vessel for use in clinical analysis including an open top, a closed bottom, and at least four tapered sides. A method for collecting magnetic particles in a fluid comprising the steps of providing a magnet and a vessel containing magnetic particles in a fluid, attracting the magnetic particles to the magnet, and moving the magnetic particles with the magnet out of the fluid.
    Type: Grant
    Filed: March 8, 2012
    Date of Patent: July 2, 2013
    Assignee: Biokit, S.A.
    Inventors: Mark Talmer, Kathleen Limerick, Gary Schroeder
  • Patent number: 8470606
    Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: June 25, 2013
    Assignees: Duke University, Advanced Liquid Logic Inc.
    Inventors: Vijay Srinivasan, Vamsee K. Pamula, Michael G. Pollack, Richard B. Fair
  • Patent number: 8465707
    Abstract: A sample handling method may include drawing an encapsulating liquid from an encapsulating-liquid input; discharging the drawn encapsulating liquid (a) onto a free surface of a carrier liquid in a carrier-liquid conduit comprising a stabilisation feature and (b) proximate to the stabilisation feature, the encapsulating liquid being immiscible with the carrier liquid, so that the discharged encapsulating liquid does not mix with the carrier liquid, floats on top of the carrier liquid, and is immobilised by the stabilisation feature; drawing a sample liquid from a sample-liquid input; and discharging the drawn sample liquid, the sample liquid being immiscible with the encapsulating liquid and with the carrier liquid, so that the sample liquid does not mix with the encapsulating liquid or with the carrier liquid.
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: June 18, 2013
    Assignee: GenCell Biosystems Ltd.
    Inventors: Kieran Curran, Paul Fleming, Séamus Gillhooley, Micheál Keane, Inga Rosca, Patrick Tuohy
  • Patent number: 8420406
    Abstract: Described is a method for analyzing the metabolites of a biological sample which comprises quantitatively determining one or more metabolites in said sample in a way that said quantitative determination resolves isotopic mass differences within one metabolite, said method being characterized in that the sample comprises or is derived from a cell which has been maintained under conditions allowing the uptake of an isotopically labeled metabolizable compound so that the metabolites in said cell are saturated with the isotope with which said metabolizable compound is labeled. This method may further comprise, prior to quantitative determining the metabolites, combining the biological sample (i.e. the first biological sample) with a second biological sample in which the metabolites are not isotopically labeled or are isotopically labeled differently from the first biological sample; and determining in said biological samples the relative quantity of metabolites which differ by their isotopical label.
    Type: Grant
    Filed: December 17, 2004
    Date of Patent: April 16, 2013
    Assignee: Max-Planck-Gessellschaft zur Forderung der Wissenschaften E.V.
    Inventors: Alexander Lüdemann, Alexander Erban, Cornelia Wagner, Joachim Kopka
  • Patent number: 8420397
    Abstract: Fluid flow devices include a small plate (2), at least one flow channel (20) formed into this small plate, at least one storage channel (221-226) extending from this connection channel, and a set of valves (V1-V6), each of which is suitable for allowing or stopping the flow of fluid in a corresponding storage channel.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: April 16, 2013
    Assignees: Rhodia Operations, Centre National de la Recherche Scientifique (C.N.R.S.)
    Inventors: Mathieu Joanicot, Philippe Laval, Jean-Baptiste Salmon
  • Patent number: 8409872
    Abstract: A cartridge for use in measuring a component to be measured contained in a sample includes a diluting well for diluting a predetermined amount of the sample to a desired dilution, and a reaction well in which the component to be measured contained in the sample and a substance specifically reacting therewith are allowed to react. A diluting solution is filled in the diluting well in a predetermined amount to provide the desired dilution based on a type of the component to be measured when the predetermined amount of the sample is dispensed in the diluting well by a uniform operation.
    Type: Grant
    Filed: October 28, 2002
    Date of Patent: April 2, 2013
    Assignee: Mitsubishi Chemical Medience Corporation
    Inventors: Hiroyuki Yokoi, Takashi Kurihara
  • Publication number: 20130064737
    Abstract: Disclosed is an automatic analyzer designed so that when it discharges a sample into an empty reaction vessel, the analyzer dispenses the sample without bringing a distal end of a sample probe into contact with a bottom surface of the reaction vessel and can thereby dispense the sample with stable dispensing accuracy in addition to extending lives of the sample probe and the reaction vessel.
    Type: Application
    Filed: December 10, 2010
    Publication date: March 14, 2013
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Takamichi Mori, Hiroaki Ishizawa, Kazumi Kusano, Isao Yamazaki
  • Patent number: 8383420
    Abstract: A method for the equilibration of enriched isotope species and natural isotope species prior to mass spectrometric analysis using solid phase and/or microwave isotope ratio equilibration and measurement.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: February 26, 2013
    Assignees: Applied Isotope Technologies, Inc.
    Inventors: Howard M. Kingston, Mizanur Rahman, David Lineman, Mehmet Pamukcu
  • Patent number: 8333938
    Abstract: Improved methods and apparatus that make more accurate and reduces risk of filling reaction chambers of cartridge cells with blood samples to conduct blood coagulation tests of the type employing the plunger technique are disclosed. A cartridge holder is provided that secures a test cartridge in a fixed upright position and deflects the plunger flag of each cartridge cell to enable manual insertion of a blood dispenser deeply into the reaction chamber to fill the reaction chamber and avoid contamination of surfaces of the cartridge outside the reaction chamber. Preferably, the cartridge holder provides illumination of the reaction chamber during filling, so that the user can judge when the reaction chamber is properly filled with blood dispensed from the blood dispenser. The cartridge holder may incorporate image magnification to facilitate viewing of the reaction chamber as it is filled.
    Type: Grant
    Filed: May 23, 2007
    Date of Patent: December 18, 2012
    Assignee: Medronic, Inc.
    Inventors: Michael M. Green, Douglas D. Nippoldt, William D. Zillmann, Brent E. Wallace, Jeff N. Rejent
  • Patent number: 8329475
    Abstract: A method for removing undesired component(s) from bound desired component(s) in an analysis including: (a) providing a container having a desired component bound thereto and an undesired component; (b) dispensing wash fluid in the container at a first level sufficient to contact a portion of the bound desired component; (c) oscillating wash fluid level in the container; and (d) removing a portion of the wash fluid from the container. A method for removing undesired components from bound desired components in an analysis including steps (a) and (b) above; (c) removing the wash fluid; (d) dispensing a wash fluid at a subsequent level lower than the first level and sufficient to wash the bound desired substrate; and (e) removing wash fluid. Preferably, the desired component is an analyte being measured.
    Type: Grant
    Filed: April 17, 2003
    Date of Patent: December 11, 2012
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Merrit N. Jacobs, Ann M. Skrobach, Andrew S. Foote, Anthony C. Machulskis
  • Patent number: 8309408
    Abstract: A method for fractionating particles includes flowing a fluid having particles therein along a first channel, applying acoustic radiation pressure to the fluid, focusing the particles within the fluid into a single file line, moving the particles in a flow rate, applying acoustic radiation pressure to the fluid for a second time, focusing the particles based on size and acoustic contrast, producing at least two fluid fractions of the particles, and collecting at least one of the fractions.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: November 13, 2012
    Assignee: Life Technologies Corporation
    Inventors: Michael D. Ward, Gregory Kaduchak
  • Patent number: 8272255
    Abstract: A system and method for preparing samples for analyte testing. The sample preparation system can include a freestanding receptacle. The method can include providing a liquid composition comprising a source and a diluent, and positioning the liquid composition in a reservoir defined by the freestanding receptacle. The method can further include filtering the liquid composition to form a filtrate comprising an analyte of interest, removing at least a portion of the filtrate from the sample preparation system to form a sample, and analyzing the sample for the analyte of interest.
    Type: Grant
    Filed: May 22, 2007
    Date of Patent: September 25, 2012
    Assignee: 3M Innovative Properties Company
    Inventors: Kurt J. Halverson, Stephen C. P. Joseph, Matthew T. Scholz
  • Publication number: 20120238034
    Abstract: An immunodiagnostic test card includes a flat planar member and at least one dilution chamber that is supported by the flat planar member. The at least one dilution chamber can be disposed adjacent chambers used for testing a patient sample that are provided on the immunodiagnostic test card or can be provided separately.
    Type: Application
    Filed: May 25, 2012
    Publication date: September 20, 2012
    Applicant: Ortho-Clinical Diagnostics, Inc.
    Inventors: Raymond Francis Jakubowicz, William G. Atterbury, Douglas E. Boyd, Michael L. Marshall
  • Patent number: 8241920
    Abstract: In at least one embodiment, the inventive technology relates to in-vessel generation of a material from a solution of interest as part of a processing and/or analysis operation. Preferred embodiments of the in-vessel material generation (e.g., in-vessel solid material generation) include precipitation; in certain embodiments, analysis and/or processing of the solution of interest may include dissolution of the material, perhaps as part of a successive dissolution protocol using solvents of increasing ability to dissolve.
    Type: Grant
    Filed: December 16, 2010
    Date of Patent: August 14, 2012
    Assignee: The University of Wyoming Research Corporation
    Inventors: John F. Schabron, Joseph F. Rovani, Jr.
  • Patent number: 8198091
    Abstract: A method for assaying the antioxidant capacity of a skin care product, the method including preparing an emulsion base, dissolving a sample of a skin care product into the emulsion base to form a homogeneous emulsion mixture, adding a detection probe to the homogeneous emulsion mixture, adding reactive oxygen species generator and/or reactive nitrogen species generator to the homogeneous emulsion mixture, measuring the fluorescence intensity change of the detection probe in the presence of the sample over time, in the presence of the standard over time, and in the presence of a blank over time, and calculating the initial rate of oxidation of the detection probe to determine the antioxidant capacity of the sample of the skin care product.
    Type: Grant
    Filed: December 24, 2008
    Date of Patent: June 12, 2012
    Assignee: Brunswick Laboratories, LLC
    Inventors: Boxin Ou, Liliang Zhang, Miwako Kondo, Hongping Ji, Yan Kou
  • Publication number: 20120142019
    Abstract: In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample isolation unit and having a diluent introduction port disposed therebetween for introduction of a diluent into the sample holding chamber. The volume within the sample holding chamber between the diluent introduction port and the sample isolation unit defines a metered volume of a sample for analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, preferably suitable for low range sample dilution, and preferably capable of being used in the point-of-care diagnostic field.
    Type: Application
    Filed: December 1, 2011
    Publication date: June 7, 2012
    Applicant: ABBOTT POINT OF CARE INC.
    Inventor: Cary James Miller
  • Publication number: 20120142020
    Abstract: In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample extraction unit, wherein a portion of said extraction unit defines a metered volume of a sample. A diluent may be transported over and/or through the extraction unit to form a diluted sample for sample analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, capable of being used in the point-of-care diagnostic field is provided. The devices and methods of the invention preferably are well-suited for high range sample dilution.
    Type: Application
    Filed: December 1, 2011
    Publication date: June 7, 2012
    Applicant: ABBOTT POINT OF CARE INC.
    Inventor: Cary James Miller
  • Patent number: 8187538
    Abstract: An immunodiagnostic test card includes a flat planar member and at least one dilution chamber that is supported by the flat planar member. The at least one dilution chamber can be disposed adjacent chambers used for testing a patient sample that are provided on the immunodiagnostic test card or can be provided separately.
    Type: Grant
    Filed: January 17, 2008
    Date of Patent: May 29, 2012
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Raymond Francis Jakubowicz, William G. Atterbury, Douglas E. Boyd, Michael L. Marshall
  • Patent number: 8158439
    Abstract: The present invention is to present a sample analyzer which is automated even when measuring a small amount sample and is capable of improving a measurement precision. The sample analyzer S comprises a sample preparation section for preparing a measurement sample; a detector D3 for detecting an analyte contained in the measurement sample; and a controller 100 being configured to 1) control the sample preparation section so as to prepare a first measurement sample of a first dilution ratio, when measuring an ordinary amount sample; and 2) control the sample preparation section so as to prepare a second measurement sample of a second dilution ratio higher than the first dilution ratio, when measuring a small amount sample.
    Type: Grant
    Filed: July 16, 2008
    Date of Patent: April 17, 2012
    Assignee: Sysmex Corporation
    Inventor: Masaharu Shibata
  • Patent number: 8097468
    Abstract: The present invention demonstrates that the diagnosis and prediction of clinical behavior in patients with hematologic malignancies, such as leukemia, can be accomplished by analysis of proteins present in a plasma sample. Thus, in particular embodiments the present invention uses plasma to create a diagnostic or prognostic protein profile of a hematologic malignancy comprising collecting plasma samples from a population of patients with hematologic malignancies; generating protein spectra from the plasma samples with or without fractionation; comparing the protein spectra with clinical data; and identifying protein markers in the plasma samples that correlate with the clinical data. Protein markers identified by this approach can then be used to create a protein profile that can be used to diagnose the hematologic malignancy or determine the prognosis of the hematologic malignancy. Potentially these specific proteins can be identified and targeted in the therapy of these malignancies.
    Type: Grant
    Filed: October 21, 2009
    Date of Patent: January 17, 2012
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Elihu H. Estey, Hagop M. Kantarjian, Francis J. Giles, Michael J. Keating
  • Patent number: 8082113
    Abstract: A sample analysis system includes a reagent preparation unit and a measurement unit. The reagent preparation unit has: a state detector which detects at least one of a state of the reagent preparation unit and a state of the reagent preparation; and a transmission unit which transmits the detected state information to a computer arranged outside the reagent preparation unit. The computer displays the received state information on a display.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: December 20, 2011
    Assignee: Sysmex Corporation
    Inventors: Takaaki Nagai, Masaharu Shibata, Kunio Ueno, Noriyuki Nakanishi
  • Patent number: 8071317
    Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium. The combination comprises (i) the sample, (ii) a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and (iii) a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent comprises a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further comprise a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties.
    Type: Grant
    Filed: February 14, 2011
    Date of Patent: December 6, 2011
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Tie Q. Wei, Alan Craig, Amy Posey