Preservative, Buffer, Anticoagulant Or Diluent Patents (Class 436/18)
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Patent number: 4727042Abstract: Disclosed are a calibrator composition and method of producing and using the same in the analysis of blood of a particular species. From whole blood of the particular species, a solution is derived with which semi- and fully-automated particle counters can be calibrated for the particular species.Type: GrantFiled: May 24, 1984Date of Patent: February 23, 1988Assignee: Southeast Vetlab Inc.Inventors: Barry T. Mitzner, Colin F. Aldersley
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Patent number: 4722904Abstract: The present invention relates to aqueous perfluorocarbon emulsions and particularly microemulsions, and to their use as blood gas controls and calibrators.Type: GrantFiled: December 18, 1986Date of Patent: February 2, 1988Assignee: Fisher Scientific CompanyInventor: Marvin C. Feil
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Patent number: 4716119Abstract: Control serum containing (1) at least one compound of the formula: ##STR1## in which T is hydrogen or methyl, hydroxymethyl or methoxymethylX is hydrogen hydroxymethyl or methyl when Y is hydroxyl, hydroxymethyl or NR.sub.2 ;Y is hydrogen, hydroxyl, hydroxylmethyl or NR.sub.2 andZ is hydroxyl or an --O--CH.sub.2 --CH.sub.2 OH group when Y is hydrogen or hydroxymethyl, or Z is hydroxymethyl or a carboxyl derivative orT is ethyl or isopropyl,X is hydrogen, or methyl, hydroxymethyl or methoxy,Y is hydroxy, hydroxymethyl or NR.sub.2 andZ is a carboxyl derivative;each R independently is hydrogen or an alkyl group with 1 to 2 carbon atoms optionally containing a hydroxy group and/or (2) at least one compound of the formulaHO(CH.sub.2 --CH.sub.2 --O).sub.n H, (II)in which n is an integer from 2 to 8,in an amount sufficient to reduce turbidities in reconstituted serum after previous lyophilization.Type: GrantFiled: October 7, 1986Date of Patent: December 29, 1987Assignee: Boehringer Mannheim GmbHInventors: Helmut Rehner, Peter Roeschlau
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Patent number: 4704352Abstract: A solution for blood cell storage having sugar-containing blood cell nutrients therein and additionally containing a magnesium or calcium L-ascorbate-2-phosphate salt is taught. The magnesium and calcium L-ascorbate-2-phosphate salts remain stable below pH 7 and thus permit sterilization of the solution at a pH at which degradation of both the L-ascorbate-2-phosphate salt and the nutrient sugar present in the solution is substantially avoided.Type: GrantFiled: June 25, 1985Date of Patent: November 3, 1987Assignee: Baxter Travenol Laboratories, Inc.Inventors: Jeffrey E. Miripol, Andrew Heaton, Richard L. Kandler
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Patent number: 4704365Abstract: A reduced dinucleotide, preferably nicotinamide adenine dinucleotide (NADH), is stabilized in an aqueous base liquid containing propylene glycol, boric acid and a buffer capable of buffering within a pH range of 8-11. The stabilized liquid contains greater than 50% (v/v) water. The remaining volume contains propylene glycol which has been chemically treated to remove oxidants. This reagent may be used by itself or in conjunction with an assay reagent system for use in the clinical laboratory.Type: GrantFiled: February 24, 1986Date of Patent: November 3, 1987Assignee: Abbott LaboratoriesInventor: David A. Yost
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Patent number: 4701417Abstract: The present invention provides a control or calibration serum for lipid diagnosis, wherein it contains the parameters relevant for lipid diagnosis in the normal or pathological concentration range in a single storage-stable form.The present invention also provides processes for the preparation of this control or calibration serum.Type: GrantFiled: November 21, 1985Date of Patent: October 20, 1987Assignee: Boehringer Mannheim GmbHInventors: Rudolf Portenhauser, Knut Bartl
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Patent number: 4693912Abstract: A method of lyophilizing reagent-coated latex particles which results in a product easily reconstituted without agglutination, retains activity, and remains stable and active at storage at 4.degree. C.Type: GrantFiled: February 28, 1985Date of Patent: September 15, 1987Assignee: Technicon Instruments CorporationInventors: Anthony M. Spadaro, Thomas J. McLoughlin, Phillip V. Engler
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Patent number: 4689308Abstract: The article includes a first vial (12) which is positioned within a second larger vial (32). The first vial (12) separately contains a first fluid, such as a first calibrating fluid, and the second vial (32) contains a second fluid, such as a second calibrating fluid. A stopper (18) seals the first vial (12) and holds a sensing portion (21) of the sensor in the first vial (12). The first vial (12) is released from the second vial (32) by activating a plunger (36).An encapsulation package (50) having first and second elements (51, 52) maintains the chemical sensor and a calibrating article or vessel in a sterile condition. The package includes a first cavity (54) which receives the calibrating vessel, the sensing portion of the sensor and a substantial portion of the sensor cable, and a second cavity (56) which receives the sensor connector. A passageway (58) connecting the two cavities (54, 56) is adapted to receive a portion of the sensor cable.Type: GrantFiled: May 1, 1986Date of Patent: August 25, 1987Assignee: International Biomedics, Inc.Inventor: Gregory J. Gerhard
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Patent number: 4684615Abstract: Isoenzyme control reagents and methods for making same stabilized by means of plexiform stabilizing means. The preferred plexiform stabilizing means is selected from the group consisting of monosaccharide and disaccharide reducing sugars. The most preferred isoenzyme control reagent comprises the isoenzyme of interest obtained from selected tissue, preclarified human sera, stabilizing cofactor, a chelating agent means, a weak nonphosphate buffer and lactose as the most preferred plexiform stabilizing means.Type: GrantFiled: September 29, 1986Date of Patent: August 4, 1987Assignee: Ciba Corning Diagnostics Corp.Inventor: Michael K. Hoskins
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Patent number: 4677075Abstract: A stable control solution is provided for the determination of urobilinogen. The control solution comprises an aqueous solution of about 3-12% ethylene glycol, about 0.1% protein and preferably about 40-400 mg/dl urobilinogen, said composition buffered to pH about 8.0-9.0 and retained in a hermetically sealed container under a blanket of inert gas.Type: GrantFiled: May 5, 1986Date of Patent: June 30, 1987Inventors: Allan L. Louderback, Paul R. Szatkowski
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Patent number: 4668618Abstract: Nuclear isolation media and procedures are described for dissociating discrete, non-agglomerated cell nuclei from animal tissue without the need to use enzyme treatments, centrifugation or the like in order to achieve the desired separation. The media facilitates separation and maintains the nuclear membrane intact and in its normal physiological environment. When a DNA-fluorochrome stain is included in the medium an essentially one step combination nuclear isolation and DNA staining procedure is used to measure DNA in tissue cells by flow cytometry. Rapid and consistent results are obtained and multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates are also described.Type: GrantFiled: January 30, 1984Date of Patent: May 26, 1987Inventor: Jerry T. Thornthwaite
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Patent number: 4663295Abstract: Reagents useful for steroid receptor assays containing both progesterone and estrogen receptors and methods. The reagents of the instant invention contain both estrogen and progesterone receptors in a stabilized format. A preferred embodiment includes target tissue material obtained from calf uteri having the required receptors, HEPES suspending buffer, a binding site activation inhibitor, dithiothreotol, plexiform stabilizing matrix means and an amount of inactive protein added as necessary to meet predetermined total protein levels.Type: GrantFiled: June 29, 1983Date of Patent: May 5, 1987Assignee: Ciba Corning Diagnostics Corp.Inventors: Martha Vail, Robert E. Megraw, Michael K. Hoskins
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Patent number: 4640897Abstract: The reagent is constituted by a suspension of basophil granulocytes bearing specific IgE's, containing from 300 to 1000 basophils per mm.sup.3. This suspension is obtained from a blood sample taken from a human or animal patient, by means of a liquid of density 1.079-1.085. It is deposited in the various wells of a diagnosis read-out slide or the like, for diagnosing parasitoses or allergies. The diagnosis method is applied particularly to worm parasitoses which causes an increase in the circulating specific IgE's. The diagnosis may be carried by using ready-for-use kits.Type: GrantFiled: May 2, 1983Date of Patent: February 3, 1987Assignee: Institut PasteurInventors: Francisque Leynadier, Herve Luce
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Patent number: 4637986Abstract: Lysing reagents for use in flow cytometry instruments. Lysing reagents suitable for lysing red blood cells are provided thereby permitting 3-part differential counting of white blood cells. The reagents comprise a leukoprotective agent for preserving the lymphocyte cellular integrity during analysis, and buffers adjusted to provide the correct pH environment for optimal lysis and activity. Preferred embodiments will additionally incorporate non-ionic preservatives in very low concentrations.Type: GrantFiled: August 22, 1983Date of Patent: January 20, 1987Assignee: Ortho Diagnostic Systems, Inc.Inventors: Michael C. Brown, Stefan J. Kirchanski
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Patent number: 4632907Abstract: A preservative solution for fixed avian erythrocytes for the viral hemagglutination test of this invention comprises purified water which may contain serum albumin, glycerol or dimethyl sulfoxide and, additionally, a preventive against the growth of bacteria as occasion demands. Fixed avian erythrocytes can stably be preserved in this solution. HA and HI test can precisely be performed by employing fixed avian erythrocytes suspended in this invention.Type: GrantFiled: August 19, 1985Date of Patent: December 30, 1986Assignee: Shionogi & Co., Ltd.Inventors: Akihiko Sato, Kunihiro Nakajima
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Patent number: 4626511Abstract: A composition which reduces the turbidity of samples of artificial or naturally occurring biological fluids comprising:a buffering agent;a nonionic surfactant;alpha-cyclodextrin;a lipase; andwater.The composition provides a catalyst for and agents which hydrolyze triglycerides present in the sample to glycerol and free fatty acids. Also present in the composition is a complexing agent or agents to render the catalyzed fatty acids water-soluble.Type: GrantFiled: May 13, 1985Date of Patent: December 2, 1986Assignee: Wayne State UniversityInventors: Joseph D. Artiss, Bennie Zak
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Patent number: 4626512Abstract: A standard solution for simultaneously calibrating a plurality of ion electrodes for determining a plurality of ion electrodes for determining a plurality of ions including at least potassium ions and calcium ions in blood, the improvement wherein the concentration of the standard solution for a potassium ion electrode and a calcium ion electrode is adjusted in accordance with the following equations: ##EQU1## As for potassium ion,C=C.sub.o .multidot.10.sup.-.DELTA.E/60As for calcium ion,C=C.sub.o .multidot.10.sup.-.DELTA.E/30As for potassium ion,A=-0.130 to -0.065As for calcium ion,A=-0.240 to -0.172whereinE: is the potential difference when an ionic strength of 150 mM is used as the standard;.mu.: is the ionic strength;C.sub.o : is the calibrated concentration;C: is the concentration of reagent added; andA: is the correction factor.Type: GrantFiled: May 22, 1985Date of Patent: December 2, 1986Assignee: Horiba, Ltd.Inventors: Narihiro Oku, Seiji Usui, Hiroaki Uematsu, Takeshi Kohno
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Patent number: 4617275Abstract: A reagent for blood analysis comprises, as a lysing reagent for leukocyte count, tetradecyltrimethylammonium bromide and/or hexadecyltrimethylammonium chloride, dodecyltrimethylammonium chloride, and citric acid. This reagent is characterized by showing three-peaks fractionation of leukocytes in measuring by means of an automatic blood-analysis instrument. The lysing reagent is used together with a diluent comprising boric acid buffer solution, ethylenediaminetetraacetic acid and (2-pyridylthio-1-oxide) sodium.Type: GrantFiled: September 24, 1984Date of Patent: October 14, 1986Assignee: Toa Medical Electronics Co., Ltd.Inventors: Noriaki Matsuda, Etsuro Shinkai
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Patent number: 4595524Abstract: Disclosed is a novel, 2 component Giemsa stain. The stain comprises a first component which is an aqueous solution of methylene blue, Azure B and a preservative and a second component which is an acetone/dimethyl formamide/water solvent system containing the sodium salt of eosin Y and a buffer. The Giemsa stain effect is obtained by first fixing a blood slide with methanol and then applying the freshly mixed first and second components of the stain. The slide is then rinsed and dried to prepare it for examination.Type: GrantFiled: March 28, 1984Date of Patent: June 17, 1986Assignee: Miles Laboratories, Inc.Inventors: Kin F. Yip, Frances F. Rhodes
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Patent number: 4590164Abstract: A process for the preparation of a glucose-free glycosylated hemoglobin standard in which hemoglobin with a known content of glycosylated hemoglobin HbA.sub.Ia+b+c is converted to cyanmethemoglobin by means of a K.sub.3 (FeCN).sub.6 /KCN solution, stabilized with a sodium phosphate-cyanide buffer and preserved by lyophilizing at -40.degree. C. with a subsequent increase in temperature to -20.degree. C. under a high vacuum and stored at this temperature with the exclusion of light, whereupon the glucose-free glycosylated hemoglobin standard may be reconstituted by the addition of an aqueous medium.Type: GrantFiled: March 26, 1984Date of Patent: May 20, 1986Assignee: Panchem GmbHInventor: Thomas Gain
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Patent number: 4572899Abstract: An aqueous solution for suspending and storing cells, especially erythrocytes, and containing sodium chloride, glucose or fructose, adenine, and a sugar alcohol, the sugar alcohol being sorbitol or xylitol is employed to further improve the storage time and hence the survival rate of the cells and even reduce the hemolysis rate of erythrocytes in packed red blood cells.Type: GrantFiled: July 6, 1983Date of Patent: February 25, 1986Assignee: Biotest-Serum-Institut GmbHInventors: Wolfram H. Walker, Karlheinz Ganshirt
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Patent number: 4555348Abstract: Monosodium phosphate is highly soluble in water and can be used as an ingredient for a low pH liquid buffer in a concentration of about 13-40 percent by weight. Tri potassium phosphate has good solubility in water and is a suitable ingredient for a high pH liquid buffer in a concentration of about 20-50 percent by weight. By a combination of these two ingredients, a system has been developed for using liquid buffer mixes in place of solid phosphate buffers, especially for use as a direct textile bath additive.Type: GrantFiled: June 28, 1984Date of Patent: November 26, 1985Assignee: Sybron Chemicals Inc.Inventor: James C. Moran
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Patent number: 4529705Abstract: An improved reagent provides combined diluting and lysing of whole blood for use in instruments performing electronic blood cell counting and hemoglobin determination. The reagent prevents platelet aggregation and improves the accuracy of the white blood cell count.Type: GrantFiled: June 6, 1983Date of Patent: July 16, 1985Assignee: Coulter Electronics, Inc.Inventor: Fred L. Larsen
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Patent number: 4528274Abstract: A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.Type: GrantFiled: March 25, 1983Date of Patent: July 9, 1985Assignee: Coulter Electronics, Inc.Inventors: James H. Carter, Stephen L. Ledis, Harold R. Crews, Ted Sena, Fred L. Larsen
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Patent number: 4521518Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.Type: GrantFiled: July 6, 1982Date of Patent: June 4, 1985Assignee: Coulter Electronics, Inc.Inventors: James H. Carter, Stephen L. Ledis, Harold R. Crews
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Patent number: 4489162Abstract: Hematologic control and calibration standards are disclosed which are essentially plasma-free. These controls are prepared by the suspension of red blood cells in an appropriate buffer containing a stabilization effective amount of disaccharides, and other optional ingredients. Control and calibration standards prepared in the foregoing manner are suitable in the control and calibration of hematology analyzers for the following parameters: white cell count, red cell count, hemoglobin, hematocrit and the common derived parameters (i.e., mean cell volume, mean cell hemoglobin and mean cell hemoglobin concentration). The addition of platelets to the foregoing suspensions is also contemplated to provide a control for platelet parameters.Type: GrantFiled: December 21, 1981Date of Patent: December 18, 1984Assignee: American Hospital Supply CorporationInventors: Pamela L. Hawkins, Carole J. Young
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Patent number: 4485174Abstract: Blood gas control containing hemoglobin, a pH buffering agent, and a reduced pyridine nucleotide capable of participating in a reaction which reduces methemoglobin to normal hemoglobin.Type: GrantFiled: September 29, 1982Date of Patent: November 27, 1984Assignee: Instrumentation Laboratory Inc.Inventors: Ching Chiang, Susan K. Bolton
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Patent number: 4480029Abstract: Biological indicators are used to evaluate the effectiveness of virus inactivation conducted on virus-contaminated, protein-containing compositions. The indicators comprise dry protein and a predetermined titer of infectious virus.Type: GrantFiled: April 27, 1981Date of Patent: October 30, 1984Assignee: Baxter Travenol Laboratories, Inc.Inventor: Gary H. Dolana
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Patent number: 4480030Abstract: A process for quantitatively assaying factor Xa from a medium by reacting the medium with tripeptide derivatives having the formula ##STR1## wherein R.sup.5 is a chromogenic substituted amino group capable of being split off by enzymatic hydrolysis to form a colored or fluorescent product H--R.sup.5. The quantity of split product H--R.sup.5 released by the enzymatic action on the tripeptide derivative is determined photometrically, spectrophotometrically, fluorescence-spectrophotometrically, or electrochemically. The quantity of released split product H--R.sup.5 per time unit is proportional to the quantity of enzyme present in the starting material.Type: GrantFiled: September 15, 1982Date of Patent: October 30, 1984Assignee: Pentapharm A.G.Inventor: Lars G. Svendsen
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Patent number: 4458021Abstract: Disclosed are cell-free, stable, aqueous blood gas controls containing a proteinaceous foaming agent. The addition of foaming agent to the blood gas control permits generation of a gas impermeable foam within the head space of a sealed ampule in which the control is packaged. Upon agitation of the ampule prior to opening, the foam thus generated effectively isolates the control fluid from the ambient environment, thereby maintaining the concentration of gases dissolved within the fluid at their assigned values.Type: GrantFiled: May 3, 1982Date of Patent: July 3, 1984Assignee: American Hospital Supply CorporationInventor: Kathryn D. Herring
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Patent number: 4431560Abstract: An acidic solution of glycine and a fatty acid of 3 to 12 carbon atoms provides an improved solution for cleaning and storing blood dialyzers and other separation devices, particularly those intended for contact with blood after they have once been used.Type: GrantFiled: December 27, 1982Date of Patent: February 14, 1984Assignee: Baxter Travenol Laboratories, Inc.Inventors: William Lake, William J. Schnell
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Patent number: 4411881Abstract: A stabilized composition comprising a solution of a radiolabeled compound and a diethylenetriamine is described. Preferred diethylenetriamines include, N,N-bis(2-aminoethyl) dithiocarbamic acid, di(2-dithiocarbamyl-ethyl) amine, and salts thereof.Type: GrantFiled: July 12, 1982Date of Patent: October 25, 1983Assignee: New England Nuclear CorporationInventor: Nathan R. Tzodikov
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Patent number: 4405719Abstract: This invention utilizes for stabilization of platelets a combination of iodoacetamide and an iminodiacetic acid or salt thereof, together with a compatible bacteriostatic agent, in an aqueous electrolytic solution which is maintained at a preselected range of pH and osmolality.In a preferred formulation, the stabilizing solution contains iodoacetamide, N-(2-acetamido)iminodiacetic acid and sodium penicillin. Ethylenediaminetetraacetic acid, or salts thereof, are optionally present as an additional ingredient.Type: GrantFiled: May 29, 1981Date of Patent: September 20, 1983Assignee: Coulter Electronics, Inc.Inventors: Harold R. Crews, James H. Carter, II, Ted Sena
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Patent number: 4403038Abstract: A method and system for measuring the oxygen carrying capacity of normal and abnormal hemoglobins and red cells under controlled pH, ionic strength, temperature and gas conditions is described. A blood sample and a physiologically balanced medium are placed in a cuvette and means are associated therewith for measuring the fractional saturation of the sample and the partial pressure of the oxygen (sometimes referred to as the oxygen tension) and provide output signals to an X-Y plotter for continuous recording. Preferably, means for maintaining the temperature of the medium and the blood sample is provided.Type: GrantFiled: September 3, 1981Date of Patent: September 6, 1983Inventors: Toshio Asakura, Horst K. Blume
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Patent number: 4389490Abstract: Stabilized reference control and calibrator compositions for determining multiple platelet parameters in stand alone platelet controls and whole blood reference controls are prepared from platelets stabilized with a fixative-stabilizing composition containing glutaraldehyde and a non-ionic surfactant which is a mixture of ethoxylates of certain isomeric linear alcohols.Type: GrantFiled: May 29, 1981Date of Patent: June 21, 1983Assignee: Coulter Electronics, Inc.Inventors: Harold R. Crews, James H. Carter, II, Ted Sena
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Patent number: 4379847Abstract: A low ionic strength suspending medium for immunologic reactions which includes a salt solution, a buffer, gelatin, albumin, and an organic solute to control osmolality.Type: GrantFiled: January 14, 1981Date of Patent: April 12, 1983Assignee: American Hospital Supply CorporationInventors: Mitchell J. Fruitstone, Michele M. Tilly, Betty G. Pixton