Involving Diffusion Or Migration Of Antigen Or Antibody Patents (Class 436/514)
  • Patent number: 10913068
    Abstract: A testing device is provided. The testing device includes a capturing tool and a microfluidic chip having a plurality of chambers connected in a network, a sample receiving port connected to the network, and a guide structure configured to receive the capturing tool, wherein the capturing tool is configured to capture sample in a distal position from the guide structure and further configured to transfer the captured sample to the sample receiving port in a proximal position from the guide structure.
    Type: Grant
    Filed: March 14, 2016
    Date of Patent: February 9, 2021
    Assignees: Nanyang Technological University, Agency For Science, Technology and Research, The Methodist Hospital
    Inventors: Joseph Sylvester Chang, Geok Soon Lim, Lei Zhang, Zhiping Wang, Ruige Wu, Stephen T. C. Wong, Kemi Cui
  • Patent number: 10832895
    Abstract: Provided is an analytical device including: a self-flowing microfluidic system, having a sample extraction location, at least one sample preparation location, and at least one sample analytical chamber; wherein the sample extraction location, the sample preparation location, and the at least one sample analytical chamber are interconnected by at least one microfluidic channel on a first substrate; and a signal readout system, having at least one sample analysis elements, and a data gathering and processing element.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: November 10, 2020
    Assignee: Plasmotica, LLC
    Inventors: Nedal Saleh, Waqas Khalid, Faisal Saleh
  • Patent number: 10788421
    Abstract: The present invention related to an electronic assay apparatus and a testing method thereof. The electronic assay apparatus for determining a result of an assay performed using a test strip comprises three light sources, only one detector and a microprocessor. The three light sources respectively illuminate light incident upon three different zones of the test strip. The detector detects light from the three zones alternately and the distances between the only one detector and the three light sources respectively are almost the same. Further, multiple openings corresponding to the three light sources respectively have the same size and/or shape. The microprocessor compares a calculating result value to only one threshold for showing a result.
    Type: Grant
    Filed: November 19, 2017
    Date of Patent: September 29, 2020
    Assignee: TAIDOC TECHNOLOGY CORPORATION
    Inventors: Chia-Chi Wu, Tien-Jung Tsai
  • Patent number: 10722883
    Abstract: A splittable fluid sample collector for dividing a fluid sample into two or more portions, includes at least first and second fluid collector portions detachably connected to one another, each of the fluid collector portions including a handle and an absorbent sample pad affixed to an end of the handle. Further, a fluid sampling apparatus includes the aforementioned splittable fluid sample collector, at least one container and a closure for the container. Still further, a method for splitting a fluid sample including providing the aforementioned splittable fluid sample collector, collecting a fluid sample with the absorbent sample pads of the splittable fluid sample collector and splitting the first and second fluid collector portions of the splittable fluid sample collector from one another.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: July 28, 2020
    Inventor: Moishe Bodner
  • Patent number: 10682829
    Abstract: Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: June 16, 2020
    Assignee: ILLUMINA, INC.
    Inventors: M. Shane Bowen, Bala Murali Venkatesan, Steven M. Barnard
  • Patent number: 10539580
    Abstract: Compositions and methods for detecting the presence and/or amount of one or more analytes, including analytes such as drugs of abuse, are provided. The compositions include two or more analytes associated with a solid phase, e.g., a particle or a multiwell plate. The compositions and methods also allow the simultaneous, tandem, or serial determination of the presence and/or amount of two or more analytes of interest in a sample.
    Type: Grant
    Filed: April 29, 2009
    Date of Patent: January 21, 2020
    Assignee: Psychemedics Corporation
    Inventors: Virginia Hill, Mohammad Atefi, Michael I. Schaffer
  • Patent number: 10436680
    Abstract: A cell capture, disruption, and extraction apparatus includes a disruption chamber configured to receive cell solution and having therein a plurality of abrasives, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The apparatus includes an actuation device configured to agitate the disruption chamber and/or pestle, movement of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. The pestle can rotate with respect to the disruption chamber. The pestle can include a nut-shaped core or axle, and/or include a plurality of extrusions. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content through the foregoing agitation process.
    Type: Grant
    Filed: October 11, 2014
    Date of Patent: October 8, 2019
    Inventor: Kianoosh Peyvan
  • Patent number: 10436781
    Abstract: A specimen delivery cartridge includes a lower housing, and an upper housing. The upper housing is coupled to the lower housing at a hinge. The specimen delivery cartridge further comprises a testing chamber comprising a paper testing substrate. The paper testing substrate may include a wicking conduit and a plurality of test areas. The specimen delivery cartridge may also include a lens assembly proximate the plurality of test areas and operable to transmit light emissions from the plurality of test areas to an image sensor of a computing device. In some embodiments, the specimen delivery cartridge includes a testing substrate having a plurality of test areas made of an array of electrodes. Each electrode is printed on a first side of the testing substrate and coupled to a conductive via formed in the testing substrate and a conductive trace printed on a second, opposing side of the testing substrate.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: October 8, 2019
    Assignee: PARATUS DIAGNOSTICS, LLC
    Inventors: John Carrano, Roland Schneider, John Jacob Carrano
  • Patent number: 10426403
    Abstract: A method of testing for specificity and strength of an allergic reaction is performed by penetrating the skin of an individual with the microneedle array comprising a plurality of epitopes and determining the response of the skin to the allergens. The response can be measured with a thermal imaging device. Analysis, and optionally treatment, can be provided to the individual.
    Type: Grant
    Filed: May 8, 2014
    Date of Patent: October 1, 2019
    Assignee: The Board of Trustees of The Leland Stanford Junior University
    Inventors: Manish J. Butte, Jayakumar Rajadas
  • Patent number: 10420337
    Abstract: An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue with a perfusate; and a sensor operatively connected to the perfusion circuit. The sensor includes a solid support to which is attached a recognition molecule that permits detection of the target agent. The recognition molecule specifically binds to the target agent in the presence of the target agent but not significantly to other agents. The sensor also includes an enzyme that can catalyze the conversion of a substance to glucose. In the presence of the target agent the enzyme can convert the substance into glucose, which can then be detected and optionally measured by the sensor.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: September 24, 2019
    Assignee: LIFELINE SCIENTIFIC, INC.
    Inventors: Steven L. Mayer, David C. Kravitz, Tracey H. Mayer
  • Patent number: 10412366
    Abstract: The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques, including imaging in three dimensions. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In some cases, the position of the entities can be determined in all three spatial dimensions (i.e., in the x, y, and z directions), and in certain cases, the positions in all three dimensions can be determined to an accuracy of less than about 1000 nm. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: September 10, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaowei Zhuang, Bo Huang, Wilfred M. Bates, Wenqin Wang
  • Patent number: 10345314
    Abstract: The present invention relates to a lateral flow membrane arrangement (1), comprising a microporous membrane layer (2) and a liquid-impermeable support layer (3), for lateral flow of a liquid through the microporous membrane layer (2), wherein the microporous membrane layer (2) is supported on the liquid-impermeable support layer (3) and has at least one detection zone (5) and at least one non-detection zone, wherein binding agents are immobilized in the at least one detection zone (5), the liquid-impermeable support layer (3) has at least one zone having a large thickness and at least one zone having a small thickness, the microporous membrane layer (2) is supported on the liquid-impermeable support layer (3) such that said at least one detection zone is provided above said at least one zone of the support layer having a large thickness and said at least one non-detection zone is provided above said at least one zone of the support layer having a small thickness, the zones are oriented in a direction orthogon
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: July 9, 2019
    Assignee: SARTORIUS STEDIM BIOTECH GMBH
    Inventors: Dieter Melzner, Denise Van Rossum
  • Patent number: 10274489
    Abstract: Assessing test results development during assay analysis is shown and described. In one embodiment, an apparatus generates an assessment of diagnostic test development from an assay. The apparatus may include an optical detector aligned in an optical path with the assay. Further, the optical detector may perform continuous image detection of the assay to generate an assessment testing development. In some examples, the assessment is a graphical display of diagnostic development. In yet other embodiments, a method of assessing diagnostic test development may include signaling the optical detector to perform continuing image detection of the assay and generating an assessment of said diagnostic test development. The assessment may include graphing a reflectance detected on the assay on a display board.
    Type: Grant
    Filed: February 11, 2014
    Date of Patent: April 30, 2019
    Assignee: Charm Sciences, Inc.
    Inventors: Robert J. Markovsky, Richard T. Skiffington, Stanley E. Charm
  • Patent number: 10258984
    Abstract: The invention relates to a cartridge (10) for processing of a liquid sample, for example for the detection of components in a sample of blood. The cartridge comprises a fluidic system with an intake port (12) leading via an intake capillary channel (13) to a storage chamber (14). Moreover, a feeding capillary channel (15) leads from the storage chamber (14) to a detection chamber (16). The design of the cartridge (10) is such that the intake capillary channel (13) that connects the intake port (12) to the storage chamber (14), has a capillary suction pressure sufficiently high to drive some sample from the intake port to the storage chamber without need of any additional pressure. Furthermore the cartridge a flow control element (18, 20) adapted to be externally controllable such that the sample can be drawn from the storage chamber (14) towards the processing chamber (16) without any active pumping.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: April 16, 2019
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Godefridus Johannes Verhoeckx, Toon Hendrik Evers, Marlieke Joan Overdijk, Monica Scholten, Nicole Henrica Maria Smulders, Joost Hubert Maas, Bernardus Jozef Maria Beerling
  • Patent number: 10254248
    Abstract: A unique fiber core sampler composition, related systems, and techniques for designing, making, and using the same are described. The sampler is used to interface with existing field instrumentation, such as Ion Mobility Spectrometer (IMS) equipment. Desired sampler characteristics include its: stiffness/flexibility; thermal mass and conductivity; specific heat; trace substance collection/release dependability, sensitivity and repeatability; thickness; reusability; durability; stability for thermal cleaning; and the like. In one form the sampler has a glass fiber core with a thickness less than 0.3 millimeter that is coated with a polymer including one or more of: polymeric organofluorine, polyimide, polyamide, PolyBenzlmidazole (PBI), PolyDiMethylSiloxane (PDMS), sulfonated tetrafluoroethylene (PFSA) and Poly(2,6-diphenyl-p-phenylene Oxide) (PPPO).
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: April 9, 2019
    Assignee: Battelle Memorial Institute
    Inventors: Raymond S Addleman, Xiaohong Shari Li, Wilaiwan Chouyyok, David A Atkinson
  • Patent number: 10247728
    Abstract: Embodiments described herein include detecting an analyte in a low pH sample. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: April 2, 2019
    Assignee: Charm Sciences, Inc.
    Inventors: Robert J. Markovsky, David W. Douglas
  • Patent number: 10207266
    Abstract: An microfluidic device includes a substrate (1) having a wandering groove (12) formed on a first surface (11) thereof; a shielding member (2) abutting on the first surface and covering the groove to form a flow channel (101) to deliver blood; an inlet (14) at one end of the flow channel for introducing the blood into the flow channel; and an outlet (16) at another end of the flow channel for discharging the blood. The flow channel (101) has a detecting region (102) for the blood passing through separately, the detecting region includes an entry (1021) for a single cell entering into at a time and an exit (1022) for a single cell exiting out at a time. A pair of electrodes (211) are disposed between the entry (1021) and the exit (1022) to detect the cells (200) of the blood.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: February 19, 2019
    Assignee: FOXCONN INTERCONNECT TECHNOLOGY LIMITED
    Inventor: Genn-Sheng Lee
  • Patent number: 10184946
    Abstract: A method for the integrated and automated analysis of DNA or protein, including a single-use cartridge, with an analysis device having a control device, and devices for capturing and processing signals, the control device carrying out a completely automatic process and evaluation of molecular diagnostic analysis via single-use cartridges (Lab-on-a-Chip). Controlling of an analysis process, which occurs in the cartridge, involve the subsequent displacement and thermostatisation of liquids with a first device, and with a second device the signals which are obtained during the analysis are processed. The first and the second devices are synchronized in such a manner that the analysis process of the sample can be carried out in a totally integrated manner thus producing an immediate result.
    Type: Grant
    Filed: August 18, 2015
    Date of Patent: January 22, 2019
    Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBH
    Inventors: Walter Gumbrecht, Peter Paulicka
  • Patent number: 10168329
    Abstract: Methods, compositions and kits for detecting Group A streptococcus in a biological sample are described. More particularly, the present disclosure provides an immunoassay in which the specificity of detection of Group A streptococcus is enhanced by addition of N-acetyl-D-glucosamine. These methods, compositions and kits are useful in convenient, reliable and early diagnosis of streptococcal infection in a human subject.
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: January 1, 2019
    Assignee: Quidel Corporation
    Inventors: Peter Yan-Guo Ren, Jason McClure, Kevin S. Richardson
  • Patent number: 10156568
    Abstract: Techniques for detection of virus-antibody nanocomplexes using a chip-based pillar array are provided. In one aspect, a method for virus detection is provided. The method includes the steps of: collecting a fluid sample from a virus-bearing source; contacting the fluid sample with an antibody that binds to viruses to form a sample-antibody mixture, wherein the antibody is labeled with a fluorescent tag; separating particles including any antibody-virus complexes, if present, from the sample-antibody mixture using an assay nanopillar array; and detecting the antibody-virus complexes, if present, in the particles from the separating step using fluorescence. A virus detection chip device and a chip-based immunoassay method are also provided.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: December 18, 2018
    Assignee: International Business Machines Corporation
    Inventors: Yann Astier, Stacey M. Gifford, Benjamin H. Wunsch
  • Patent number: 10145841
    Abstract: The present invention provides a method for detecting an antigen in an analysis sample, the method including: (a) contacting an analysis sample with a detection antibody with which a marker generating a detectable signal is combined and which specifically binds to the antigen; (b) contacting a capture antibody with the resultant product of step (a), the capture antibody specifically binding to an antigen to be detected; (c) contacting the detection antibody, with which the marker generating a detectable signal is combined, with a reference substance which is bound to a surface of a solid substrate and which includes an epitope to which the detection antibody specifically binds; (d) measuring signals generated from the markers of the resultant product of step (b) and the resultant product of step (c); and (e) analyzing the measured signals to determine the presence or absence and amount of the antigen in the analysis sample.
    Type: Grant
    Filed: February 20, 2013
    Date of Patent: December 4, 2018
    Assignee: NANOENTEK, INC.
    Inventors: Chan Il Chung, Chang Seop Lee, Jeoung Ku Hwang, Jong Sik Jeong
  • Patent number: 10101342
    Abstract: An improved qualitative or semi-quantitative diagnostic test for measuring low levels of any analyte, such as hCG, in a biological sample, such as urine. The test comprises a test device containing reagents for the detection of the monitored analyte and an electronic reader that measures color development at a detection area of the device. The color development is converted to an electronic or digital signal. Improvements were made to the electronic reader to optimize the coverage of the detection area on the test strip. This improves the detection sensitivity and consistency of the test result while maintaining its reliability and accuracy.
    Type: Grant
    Filed: February 12, 2014
    Date of Patent: October 16, 2018
    Assignee: Church & Dwight Co., Inc.
    Inventors: Albert R. Nazareth, Benedict Zin, Andy Sturman, Shang Li
  • Patent number: 10073092
    Abstract: A lateral flow assay device that is defined by multiple loading zones, wherein a sample pad is located before and upstream from a conjugate pad and another sample pad is located after and downstream from the conjugate pad. This configuration allows for increased sensitivity for detecting an analyte in a specimen. The device may employ multiple collection pads or a single collection pad and the collection pads may be engaged with the sample pads in several manners, including but not limited to, pressing down on a cassette with collection pads onto a cassette with the test strips, or by sliding the collection pads along the cassette with the test strips and sample pads.
    Type: Grant
    Filed: February 19, 2016
    Date of Patent: September 11, 2018
    Inventor: Andrew Wang
  • Patent number: 9903799
    Abstract: Devices and methods are presented in which a plasma separation device with a first and second portion separates a blood containing fluid. Most preferably, the first portion produces a cell fraction and a plasma fraction, and the second portion captures the plasma fraction. A first actuator then fluidly isolates a portion of the plasma fraction within the second portion, and a second actuator moves the isolated portion of the plasma fraction from the second portion.
    Type: Grant
    Filed: April 20, 2017
    Date of Patent: February 27, 2018
    Assignee: Qualigen, Inc.
    Inventors: Michael Poirier, John Stephen Middleton, Suzanne Marie Poirier
  • Patent number: 9891216
    Abstract: Methods are provided for determining the presence of a first ligand in a sample. In some embodiments depletion conjugates are used to deplete the ligands different from but related to the first ligands from the sample. In some embodiments, interim binding agents are used to enhance the test signal.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: February 13, 2018
    Assignee: CHEMBIO DIAGNOSTIC SYSTEMS, INC.
    Inventor: Javanbakhsh Esfandiari
  • Patent number: 9885710
    Abstract: Test devices are provided for determining the presence of a first ligand in a sample. In some embodiments depletion conjugates are used to deplete the ligands different from but related to the first ligands from the sample. In some embodiments, interim binding agents are used to enhance the test signal.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: February 6, 2018
    Assignee: Chembio Diagnostic Systems, Inc.
    Inventor: Javanbakhsh Esfandiari
  • Patent number: 9874557
    Abstract: A cartridge and method for conducting a labeled molecular affinity binding test such as antibody/antigen, ligand/receptor, and colorometric reactions, and other chemical reactions for which one or more analytes is present in a liquid sample formate. A progressive compression structure progressively forces a liquid flow out of a conjugate pad toward a reaction region to more thoroughly and rapidly mix the liquid, encouraging specific first affinity binding to the analytes in question. A specially dimensioned constricting passageway surrounding the reaction region including the result zones provides an additional siphoning force to the flow. These combined forces rapidly and more evenly guide the flow of liquid through the reaction region so that the rushed rate of uptake of analytes at the strip lines are more evenly distributed, adhesive attachment of non-specific molecules is largely avoided, vastly improving sensitivity and specificity, and providing quantitative results in some tests.
    Type: Grant
    Filed: June 21, 2016
    Date of Patent: January 23, 2018
    Assignee: DNT Scientific Research, LLC
    Inventors: Naishu Wang, David F. Zhou
  • Patent number: 9857385
    Abstract: The present disclosure provides an isolated particle comprising very high density, ultra small, lipid depleted apo B containing particles, and may also contain cytokeratin 8. The isolated particle is useful in diagnostic assays, which are also provided.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: January 2, 2018
    Assignee: Children's Hospital & Research Center at Oakland
    Inventors: Ronald M. Krauss, Mohmed Elfatih Ashmaig
  • Patent number: 9850318
    Abstract: Disclosed is an antibody which binds to quetiapine, which can be used to detect quetiapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of quetiapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
    Type: Grant
    Filed: August 20, 2013
    Date of Patent: December 26, 2017
    Assignee: Janssen Pharmaceutica NV
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Matthew Garrett Donahue, Yong Gong
  • Patent number: 9851364
    Abstract: Disclosed is a method for determining a quantitative estimate of the length of time since conception in a female mammalian subject, the method comprising: a) providing a liquid sample suspected of containing hCG; b) measuring, by means of an assay or assay device, an analyte measurement signal, whose value is dependent upon the level of hCG; c) comparing the measured signal value to an analyte threshold, wherein said analyte threshold corresponds to a time since conception; d) providing an quantitative estimate of the length of time since conception based upon the comparison in step (c).
    Type: Grant
    Filed: June 3, 2009
    Date of Patent: December 26, 2017
    Assignee: ALERE SWITZERLAND GMBH
    Inventors: Jayne Ellis, Jayne Marshall, Stephen P. Sharrock
  • Patent number: 9821249
    Abstract: Disclosed is a continuous process for separating or extracting proteins from a low grade mixture of a protein of interest, other proteins, impurities, and salts in a continuous simulated moving bed separation process. The invention provides for direct extraction of heme protein and plant protein from a crude mixture of such proteins, other proteins, impurities and salts using the chromatographic technique of simulated moving bed (SMB) continuous chromatography. The SMB process combines the steps of feed loading, adsorbent washing, product elution, adsorbent regeneration, and adsorbent equilibration. The novel strong anion exchange resin adsorbent is a quaternary amine cross-linked microcellulose wherein the microcellulose is cross-linked with epichlorohydrin and the quaternary amine is 2,3-epoxypropyltrimethyl-ammonium chloride which exhibits selective adsorption of proteins and complete regeneration.
    Type: Grant
    Filed: September 8, 2015
    Date of Patent: November 21, 2017
    Assignee: OROCHEM TECHNOLOGIES, INC.
    Inventors: Anil R. Oroskar, Babu Siddegowda Antharavally, Anantha Krishna Mallia
  • Patent number: 9804161
    Abstract: Detectors and related methods and systems are described, based on a primary binding compound and a secondary binding compound used in combination with a support to detect a target in a sample.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: October 31, 2017
    Assignee: Lawrence Livermore National Security, LLC
    Inventor: Jane P. Bearinger
  • Patent number: 9759724
    Abstract: Disclosed is a test device and a method for qualitatively and/or quantitatively measuring the concentration of an analyte in a biological fluid sample. The test device includes a housing defining a sample port, a test well containing a stirrer and a conjugate, and a test strip disposed within the housing. The test well is also defined by being located between the sample port and the test strip. Fluid flows from the test well onto the test strip, which has a trapping zone which binds the analyte and allows for its detection. A control zone may also be included. The test device is generally adapted to use a sandwich assay. Also disclosed is a system comprising the test device and a signal sensing device; and a method for using the test device.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: September 12, 2017
    Assignee: INSTANT MEDICAL DIAGNOSTICS, LLC
    Inventors: Alan R. Day, Allan M. Weinstein, Bryan C. Christiansen
  • Patent number: 9712805
    Abstract: The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques, including imaging in three dimensions. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In some cases, the position of the entities can be determined in all three spatial dimensions (i.e., in the x, y, and z directions), and in certain cases, the positions in all three dimensions can be determined to an accuracy of less than about 1000 nm. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: July 18, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaowei Zhuang, Bo Huang, Wilfred M. Bates, Wenqin Wang
  • Patent number: 9664668
    Abstract: Devices and methods are presented in which a plasma separation device with a first and second portion separates a blood containing fluid. Most preferably, the first portion produces a cell fraction and a plasma fraction, and the second portion captures the plasma fraction. A first actuator then fluidly isolates a portion of the plasma fraction within the second portion, and a second actuator moves the isolated portion of the plasma fraction from the second portion.
    Type: Grant
    Filed: May 3, 2012
    Date of Patent: May 30, 2017
    Assignee: QUALIGEN, INC.
    Inventors: Michael Poirier, John Stephen Middleton, Suzanne Marie Poirier
  • Patent number: 9645060
    Abstract: Devices and methods are disclosed herein for separating a supernate from a suspension. The apparatus consists of a sample zone, a controllable gate, and an analysis zone. The sample zone holds the suspension. The analysis zone passively transports a supernate formed from the suspension by capillary transport. A controllable gate prevents the suspension in the sample zone from flowing into the analysis zone. The controllable gate can be triggered after the supernate has separated from the suspension to allow the supernate to flow into the analysis zone.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: May 9, 2017
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventor: Jason O. Fiering
  • Patent number: 9611332
    Abstract: Disclosed is an antibody which binds to aripiprazole, which can be used to detect aripiprazole in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of aripiprazole, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
    Type: Grant
    Filed: August 20, 2013
    Date of Patent: April 4, 2017
    Assignee: Janssen Pharmaceutica NV
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M Remmerie, Rhys Salter, Ronghui Lin
  • Patent number: 9606039
    Abstract: A flow cytometry system suitable for characterizing multicellular aggregates during culture and before implantation combines a low shear flow channel with a multiphoton laser scanning microscope, the latter permitting the characterization of interior and exterior cells in optical isolation from other cells for a representative sampling of fluorescent activity. Imaging capabilities permit sophisticated statistical measurements reflecting individual cell characteristics.
    Type: Grant
    Filed: September 16, 2009
    Date of Patent: March 28, 2017
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brenda M. Ogle, Luis A. Fernandez, Kevin W. Eliceiri, Matthew S. Hanson
  • Patent number: 9494583
    Abstract: The disclosure relates to a method of detecting a structural change in a molecule that is attached to a surface that is electrically conductive, wherein the method includes monitoring the phase of the electrochemical impedance at the surface, and wherein a change in the phase indicates a change in the structure of the molecule. The disclosure also relates to arrays and methods for making arrays having molecules, such as polypeptides, attached to electrically conductive surfaces, such as electrodes.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: November 15, 2016
    Assignee: UNIVERSITY OF LEEDS
    Inventors: Paul Ko Ferrigno, Christoph Walti, David Evans, Steven Johnson, Alexander Giles Davies
  • Patent number: 9488657
    Abstract: Analyte detection systems and devices are provided. In one embodiment, a sensor initially reviews an assay, for instance a test strip, to designate a particular testing sequence. The sensor may calibrate the unit and then identify the particular testing sequence by identifying a color indicator on the test strip. The result is systems and methods to improve the detection of the presence and/or absence of at least one analyte in a sample.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: November 8, 2016
    Assignee: Charm Sciences, Inc.
    Inventors: Paul E. Graham, Robert J. Markovsky
  • Patent number: 9290549
    Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: March 22, 2016
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Martin Hall, Sture Larsson, Andreas Muranyi, Gustav Rodrigo, Jinyu Zou, Per-Mikael Aberg
  • Patent number: 9227189
    Abstract: A microfluidic liquid stream configuration system is provided including providing a substrate; forming a first co-planar electrode and a second co-planar electrode on the substrate; applying a dielectric layer, with a controlled surface energy, on the first co-planar electrode and the second co-planar electrode; forming an input reservoir on the first co-planar electrode and a second co-planar electrode; supplying a liquid in the input reservoir for analysis; and imposing an electric field, an electric field gradient, or a combination thereof on the liquid for respectively driving surface charge or dipole moments in the liquid for configuring a liquid stream.
    Type: Grant
    Filed: August 23, 2006
    Date of Patent: January 5, 2016
    Assignee: Zymera, Inc.
    Inventors: Daniel Sobek, Jun Zeng
  • Patent number: 9207241
    Abstract: The present invention relates generally to an assay for detecting and differentiating multiple analytes, if present, in a single fluid sample, including devices and methods therefore.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: December 8, 2015
    Assignee: Quidel Corporation
    Inventors: Paul Lambotte, Michael Cerrito, Cecille Ramirez
  • Patent number: 9182418
    Abstract: A bodily fluid analyzer including a dry test strip impregnated with a reagent providing a non-precipitating reaction to exclude non-desired analytes. The reagent complexes the non-desired analytes so they remain in solution but cannot participate in the test reaction. Red blood cells are removed from the detection area by slowing their vertical movement and stopping flow when the detection membrane is saturated.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: November 10, 2015
    Assignee: Polymer Technology Systems, Inc.
    Inventors: Gregory M. Lawrence, Meredith Knight
  • Patent number: 9179895
    Abstract: According to a first aspect of the present invention there is provided an oral fluid collection device. The oral fluid collection device comprises a handle, a swab holder, and a swab. The swab is in the form of a hollow tube of absorbent material coaxially located about the swab holder, such that the swab is supported by the swab holder along the entire length of the swab.
    Type: Grant
    Filed: March 16, 2010
    Date of Patent: November 10, 2015
    Assignee: Concateno UK Limited
    Inventors: Gordon Jowett, Andrew Woodhead
  • Patent number: 9151753
    Abstract: A method, test strip and method of manufacturing a test strip useful for detecting one or more analytes, such as an antibiotic, in a test sample such as a milk sample. The test strip and method include a labeled specific binder and test capture agent for the specific binder that increases test sensitivity to the analyte for which the specific binder has affinity while decreasing test sensitivity to an analyte for which a multianalyte binder has affinity.
    Type: Grant
    Filed: June 18, 2010
    Date of Patent: October 6, 2015
    Assignee: Charm Sciences, Inc.
    Inventors: David W. Douglas, Robert J. Markovsky
  • Patent number: 9121855
    Abstract: This invention concerns methods for detecting the presence of an anti-HIV-1 antibody in a biological sample, the method comprising conducting an immunoassay comprising: (a) contacting the biological sample with at least one epitope that is recognized by the anti-HIV-1 antibody, wherein the contacting being under conditions sufficient to permit the anti-HIV-1 antibody if present in the sample to bind to the epitope and form an epitope-anti-HIV-1 antibody complex; (b) contacting the formed epitope-anti-HIV-1 antibody complex with an anti-HIV-1 antibody binding molecule, the contacting being under conditions sufficient to permit the anti- HIV-1 antibody binding molecule to bind to anti-HIV-1 antibody of the formed epitope-anti-HIV-1 antibody complex and form an extended complex; and (c) determining the presence or concentration of the anti-HIV-1 antibody in the biological sample by determining the presence or concentration of the formed extended complex; the epitope being present on a peptide comprising SEQ ID N
    Type: Grant
    Filed: December 23, 2010
    Date of Patent: September 1, 2015
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Hana Golding, Surender Khurana
  • Patent number: 9116146
    Abstract: A method of determining the concentration of a test fluid sample using a paper-based microfluidic system having a plurality of hydrophilic testing zones, including: a) depositing said test fluid sample on at least one said testing zone; b) depositing a plurality of standard fluid samples or reactives of differing known concentrations on other said testing zones; c) introducing an indicator solution to each said test zone to thereby react with the deposited fluid sample and result in a color intensity change which is a function of the fluid sample concentration; and d) comparing the differences in color intensity between the test fluid sample and the standard fluid samples or reactives to thereby determine the concentration of said test fluid sample.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: August 25, 2015
    Assignee: Monash University
    Inventors: Wei Shen, Xu Li, Junfei Tian, Gil Garnier
  • Patent number: 9079179
    Abstract: A microfluidic device for performing detection of a substance in a liquid sample includes a cavity (24) formed above a sensor surface area (22) of a sensor (18). The cavity (24) extends at the first side of a base plate (10) from a first area (32), where the cavity overlaps a first lateral channel part (30), to a second area (34), where the cavity overlaps a second lateral channel part (36). The second lateral channel part (36) includes a lateral channel part formed by a porous capillary suction structure (13). The cavity (24) forms a flow path (42) from the first lateral channel part (30) along the sensor surface area (22) to the second lateral channel part (36).
    Type: Grant
    Filed: April 9, 2010
    Date of Patent: July 14, 2015
    Assignee: Koninklijke Philips N.V.
    Inventors: Reinhold Wimberger-Friedl, Christiane Rossetta Maria De Witz
  • Patent number: 9081009
    Abstract: Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, oxidized cardiolipins, which may be conjugated with a variety of attachment molecules, such as BSA, KLH, biotin, synthetic protein MAPS, IgY, streptavidin, or avidin, are described. Such oxidized cardiolipin, alone or complexed with one or more attachment molecules, are useful to detect anti-lipoidal antibodies (such as IgG and IgM antibodies) in subjects, for example, when used in ELISA plates. ELISA plates are described that permit the detection of anti-lipoidal antibodies and that permit the co-detection of nontreponemal and treponemal antibodies in biological samples.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: July 14, 2015
    Assignees: The United States of America as represented by the Secretary of the Department of Health and Human Services, Arlington Scientific Inc.
    Inventors: Arnold R. Castro, Himanshu Champaklal Mody