Involving Diffusion Or Migration Of Antigen Or Antibody Patents (Class 436/514)
  • Patent number: 11860172
    Abstract: The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a lipid containing sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis. Due to the use of a liquid phase that does not destroy native tissue morphology during analysis, a subsequent staining technique can be performed on the tissue sample and an overlaid image can be produced of a mass spectral image and a staining image.
    Type: Grant
    Filed: May 26, 2021
    Date of Patent: January 2, 2024
    Assignee: Purdue Research Foundation
    Inventors: Robert Graham Cooks, Livia Schiavinato Eberlin, Christina Ramires Ferreira, Allison Lisa Dill, Demian R. Ifa
  • Patent number: 11846637
    Abstract: A device for determining presence or absence of infection due to an infectious agent is described. The device comprises a sample receiving zone configured to receive a liquid sample from a subject suspected of having an infection due to an infectious agent, the sample receiving zone positioned to distribute the sample along a first fluid flow path to a first label zone and along a second fluid flow path to a second label zone. Test lines in each fluid flow path capture a mobile detectable species as an indicator of presence or absence of the infectious agent. The device also comprises a reference line positioned in the one of the fluid flow paths. The bidirectional fluid flow paths emanate from a common sample zone and provide an efficient approach to detection and differentiate two species as indicators of the same infectious agent or as indicators of two different infectious agents.
    Type: Grant
    Filed: August 24, 2021
    Date of Patent: December 19, 2023
    Assignee: Quidel Corporation
    Inventors: Robert Reed, Irene Sinn-Blandy, Robert Weiller, Jason McClure
  • Patent number: 11807893
    Abstract: A method is provided for determining, the presence and concentration of an analyte by contacting the sample with a solution comprising: magnetic beads, a capture probe capable of binding the analyte, a reporter probe and cellulose, whereby, if the analyte is present, an MB-analyte-reporter-cellulase sandwich is formed; and then contacting the solution comprising the sandwich with an electrode covered with an electrically insulating layer comprising or consisting of cellulose and/or a cellulose derivative, wherein the MB-analyte-reporter-cellulase sandwich leads to degradation of the insulating layer thereby causing a measurable change in electrical properties at the electrode surface, wherein the change in electrical properties is a function of the amount of analyte in the sample. Devices and biosensor applying the method are also provided.
    Type: Grant
    Filed: July 2, 2018
    Date of Patent: November 7, 2023
    Assignee: Numen Sensorics ApS
    Inventor: Elena Ferapontova
  • Patent number: 11796542
    Abstract: Provided is a test system including at least the following: permeabilising means and/or lysis of at least one pathogen and/or at least one cell, means for capturing and/or making parts of the pathogens and/or cells, means for localising, immobilising and/or enriching at least one component of a pathogen and/or a cell, means for image processing preferably including an optical magnifying unit, enabling an optical reading out of at least one means for localising, immobilising and/or enriching can be carried out.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: October 24, 2023
    Assignee: Zendia GmbH
    Inventors: Guido Böse, Markus Ganter, Taleieh Rajabi, Andreas Guber, Ralf Ahrens
  • Patent number: 11772094
    Abstract: Provided are a microfluidic apparatus and a method for separating a target cell using the same. The microfluidic apparatus for separating a first material in a biological sample from the biological sample, according to an embodiment of the disclosure, includes: a body rotatable on a rotation axis; a mixing chamber included in the body, wherein the biological sample and magnetic beads that are combined with a second material in the biological sample are mixed in the mixing chamber; a separation chamber included in the body and connected to the mixing chamber, wherein a mixed sample of the first material and the second material combined with the magnetic beads is separated in the separation chamber; and a magnetic member positioned on one side of the body outside the separation chamber, wherein, in the separation chamber, the first material is separated from the second material combined with the magnetic beads by a centrifugal force and a magnetic force.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: October 3, 2023
    Assignee: DAEGU GYEONGBUK INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventor: Min Seok Kim
  • Patent number: 11761958
    Abstract: Devices, systems, and methods are provided for magnetic focus enhanced lateral flow assays. The devices and systems are ultrasensitive and provide for the visual detection of the presence or absence of one or more target analytes—which may include pathogens, proteins, or even molecules smaller than the foregoing—even when such analytes are only present in very limited amounts. The devices and systems include an immunostrip with a magnet positioned adjacent thereto, and magnetic probes specific to a target analyte that bind to the target analyte with specificity if present within a fluid sample to be tested. Methods are also provided for detecting one or more target analytes using magnetic focus, such methods including a step of controlling movement of a target analyte complex on an immunostrip incorporating a magnetic field, where such control slows a flow of the target analyte complex through a capture area on the immunostrip.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: September 19, 2023
    Assignee: Purdue Research Foundation
    Inventors: Joseph M K Irudayaraj, Wen Ren
  • Patent number: 11754543
    Abstract: A simple, sensitive method and device for detecting the LEAD presence in the water. The test involves a sample of water from a subject; followed by determining the presence of LEAD in the water sample by using a LEAD sensitive solution used in the device.
    Type: Grant
    Filed: February 25, 2021
    Date of Patent: September 12, 2023
    Inventor: Mirza Faizan
  • Patent number: 11739366
    Abstract: Methods are provided for detection of a target cell type within a cell population, and compositions are provided comprising cells and an indicator that indicates the number of cells of the target cell type in the cell population. Examples are provided in which these methods are used to detect human embryonic stem cells within a differentiated cell population with exquisite sensitivity. Differentiated cells produced from embryonic stem cells can be characterized by these methods before transplantation into a recipient, thereby providing further assurance of safety.
    Type: Grant
    Filed: July 25, 2011
    Date of Patent: August 29, 2023
    Assignee: Astellas Institute for Regenerative Medicine
    Inventors: Irina V. Klimanskaya, Roger Gay
  • Patent number: 11733241
    Abstract: A resin-metal composite to be used as a marker in an immunoassay, said resin-metal composite comprising a resin particle and metal particles and having the following constitution (A) or constitution (B): (A) the average particle size of the resin-metal composite exceeding 300 nm; or (B) the average particle size of the metal particles being in the range of more than 20 nm and less than 70 nm. It is preferred that a part of the metal particles are two- or three-dimensionally distributed in the surface layer part of the resin particle, a part of the three-dimensionally distributed metal particles are partly exposed to the outside of the resin particle, and a part of the remainder particles are enclosed in the resin particle.
    Type: Grant
    Filed: April 5, 2020
    Date of Patent: August 22, 2023
    Assignee: NIPPON STEEL Chemical & Material Co., Ltd.
    Inventors: Yasufumi Matsumura, Yasushi Enomoto, Ryuzo Shinta
  • Patent number: 11730456
    Abstract: According to a first aspect of the invention, there is provided a device for estimation of an ovulation date, comprising a camera, configured to obtain colour data of successive FSH test strips and a processor, configured to evaluate the colour data obtained by the camera. The processor is configured to determine a concentration value of each successive FSH test strip based on the data of the camera and to output a signal, if the processor determines that a first FSH downward trend of the FSH test strip values is occurring in the successive FSH test strips.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: August 22, 2023
    Assignee: COLORIMETRIX GmbH
    Inventor: Juan Leonardo Martinez Hurtado
  • Patent number: 11692160
    Abstract: The microfluidic chip according to an embodiment of the present invention may include a plate, a bridge channel formed in intaglio on one side of the plate, an inlet formed through the plate to communicate with one end of the bridge channel, an outlet formed through the plate to communicate with the other end of the bridge channel, and at least one well extending in an outward direction of the plate from the bridge channel to provide a space, wherein the bridge channel may be in the form of a curved line, a bent line, an arc, a circle, a spiral, or a polygon.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: July 4, 2023
    Assignees: THE ASAN FOUNDATION, UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION
    Inventors: Gi Seok Jeong, Changmo Hwang, Se-Jin Jang
  • Patent number: 11680949
    Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
    Type: Grant
    Filed: March 1, 2021
    Date of Patent: June 20, 2023
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Michael P. Caulfield, Richard E Reitz, Shuguang Li, Gloria Kwangja Lee, Ronald Krauss, Patricia J. Blanche, W. Henry Benner, Earl Cornell
  • Patent number: 11666946
    Abstract: There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.
    Type: Grant
    Filed: September 8, 2021
    Date of Patent: June 6, 2023
    Assignee: SONY GROUP CORPORATION
    Inventor: Tatsumi Ito
  • Patent number: 11668714
    Abstract: A solid support for detecting the presence of antibodies in a biological sample, where the solid support includes microbial antigens immobilized on the solid support, wherein the microbial antigens include at least one antigen prepared from the group consisting of pleomorphic round bodies of Borrelia genus, for example Borrelia burgdorferi, Borrelia afzelii and Borrelia garinii. Also, a method of detecting a tick-borne microbe in a biological sample, wherein the solid support is contacted with a biological sample.
    Type: Grant
    Filed: May 24, 2022
    Date of Patent: June 6, 2023
    Assignee: Te?ted Oy
    Inventors: Leona Gilbert, Kunal Garg, Leena Meriläinen, Kanoktip Puttaraksa
  • Patent number: 11602748
    Abstract: The present invention relates to the field of micro fluidics. Specifically, the present invention relates to a novel flow cell for the selective enrichment of target particles or cells from a fluid. The flow cell exhibits a novel design which greatly improves the target particle or cell yield. The invention also provides a micro fluidic device, comprising the flow cell according to the invention. In another aspect, the invention relates to the use of a flow cell or a micro fluidic device of the invention for the isolation of target particles or cells from a fluid sample. Finally, the invention relates to a method for the selective enrichment of target particles or cells from a fluid using the flow cell of the invention.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: March 14, 2023
    Assignee: Xantec Bioanalytics GmbH
    Inventors: Erk Tjalling Gedig, Melanie Dieckmann
  • Patent number: 11519910
    Abstract: The present invention provides a diagnostic kit for detecting the presence or quantity of one or more test analytes within a test sample taken from a body surface of a mammal, the diagnostic kit comprising: a separate insert for a lateral flow device (200, 411) comprising a membrane (201) fixed to a rigid support (202) and, the separate insert being configured to obtain the test sample; a lateral-flow assay device configured (300, 400) to accept the separate insert (200, 411); a securing member (210) configured to releasably attach (211) the separate insert to a body surface of a mammal (213); wherein the securing member (210) comprise an expandable layer (212) configured to apply pressure to the separate insert (200, 411) thereby pressing the separate insert (200, 411) against the body surface of the mammal (213).
    Type: Grant
    Filed: May 12, 2014
    Date of Patent: December 6, 2022
    Assignee: FIBROTX OÜ
    Inventor: Toomas Neuman
  • Patent number: 11504709
    Abstract: A cell capture, disruption, and extraction method includes a introducing a plurality of abrasives in a disruption chamber, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The method includes agitating the abrasives by moving the disruption chamber and/or pestle, agitation of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content. The lysate can then bind to an extraction matrix downstream of the disruption chamber or it can be mixed in with the abrasives.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: November 22, 2022
    Inventor: Kianoosh Peyvan
  • Patent number: 11493511
    Abstract: A system, apparatus, and method for registering and interpreting the results of lateral flow assay determination by using electric, magnetic, and RF sensors incorporated within the test strip, attached to the inside of the enclosure for same and/or contained in a test fixture; instead of relying on optical inspection techniques. This method features high reliability, low cost, and ability for quantitative and dynamic measurements.
    Type: Grant
    Filed: September 4, 2018
    Date of Patent: November 8, 2022
    Inventors: Sameh Sarhan, Lawrence Herbert Zuckerman
  • Patent number: 11474039
    Abstract: The chemical sensing device comprises a substrate of semiconductor material, integrated circuit components and a photodetector formed in the substrate, a dielectric on the substrate, a wiring in the dielectric, and a source of electromagnetic radiation, a waveguide and a fluorescent sensor layer arranged in or above the dielectric. A portion of the waveguide is arranged to allow the electromagnetic radiation emitted by the source of electromagnetic radiation to be coupled into the waveguide. A further portion of the waveguide is arranged between the photodetector and the fluorescent sensor layer.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: October 18, 2022
    Assignee: ams AG
    Inventors: Martin Sagmeister, Victor Sidorov, Jochen Kraft
  • Patent number: 11420201
    Abstract: A lateral flow testing device includes a main body adapted to support an element for collecting a sample. The element is supported at a distal end of the testing device and is in fluid communication with one or more testing components having an analyte detection zone for detecting one or more analytes present in said sample. The testing components produce visual information related to said sample, thereby informing the user of the outcome of a test. The device incorporates a handle reconfigurable with respect to said main body such that in a first handle configuration, the handle hinders sight of the visual information to improve privacy of the test, and in a second handle configuration, the handle facilitates the collection of said sample by increasing a distance between a proximal end of the testing device and the element.
    Type: Grant
    Filed: October 10, 2019
    Date of Patent: August 23, 2022
    Assignee: SPD Swiss Precision Diagnostics GMBH
    Inventors: Paul J. Laverack, James Gani
  • Patent number: 11413618
    Abstract: The present invention provides a detection device, and the device comprises a testing element, wherein the testing element comprises a detection area used for detecting a presence of an analyte in a liquid sample; and a transparent area through which the test result on the detection area is read, and the transparent area includes a hydrophilic area and a hydrophobic area. Thus, the detection device reduces formation of droplets on the transparent area is reduced, that is, it avoids formation of a mist layer on the transparent area; or avoids accumulation of small droplets on the transparent area, thus to make the result on the test area be clearly read.
    Type: Grant
    Filed: July 29, 2020
    Date of Patent: August 16, 2022
    Assignee: HANGZHOU BIOTEST BIOTECH CO., LTD.
    Inventors: Shujiang Wu, Liang Hong, Jiang Liu, John Wu, Yangyu Zhu, Junsheng Wu
  • Patent number: 11391746
    Abstract: A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.
    Type: Grant
    Filed: June 7, 2016
    Date of Patent: July 19, 2022
    Assignee: MONASH UNIVERSITY
    Inventors: Gil Garnier, Wei Shen, Mohidus Samad Khan, Xu Li, George Thouas
  • Patent number: 11389800
    Abstract: The present invention generally relates to systems and techniques for manipulating fluids and/or making droplets. In certain aspects, the present invention generally relates to droplet production. The droplets may be formed from fluids from different sources. In one set of embodiments, the present invention is directed to a microfluidic device comprising a plurality of droplet-making units, and/or other fluidic units, which may be substantially identical in some cases. Substantially each of the fluidic units may be in fluidic communication with a different source of a first fluid and a common source of a second fluid, in certain embodiments. In one aspect, substantially the same pressure may be applied to substantially all of the different sources of fluid, which may be used to cause fluid to move from the different sources into the microfluidic device. In some cases, the fluids may interact within the fluidic units, e.g., by reacting, or for the production of droplets within the microfluidic device.
    Type: Grant
    Filed: September 27, 2012
    Date of Patent: July 19, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Ralph Alexander Sperling, Adam R. Abate, David A. Weitz
  • Patent number: 11376595
    Abstract: The present invention discloses a droplet digital PCR chip. The droplet digital PCR chip includes at least one chip unit, each chip unit includes a chip body formed by bonding a top piece and a bottom piece, the chip body is internally provided with an inlet chamber, a droplet storage chamber, and an injection hole. The injection hole connects with the inlet chamber, a plurality of droplet generating channels are disposed between the inlet chamber and the droplet storage chamber, a height of the droplet generating channel is smaller than a height of the droplet storage chamber, an injection fluid is injected into the inlet chamber through the injection hole, and the injection fluid is emulsified and enters the droplet storage chamber at a junction of the droplet generating channels and the droplet storage chamber.
    Type: Grant
    Filed: November 30, 2017
    Date of Patent: July 5, 2022
    Assignee: Pilot Gene Technologies (Hangzhou) Co., Ltd.
    Inventors: Jian Shi, Shuanghong Xiang, Xiaohui Song
  • Patent number: 11358147
    Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
    Type: Grant
    Filed: May 28, 2021
    Date of Patent: June 14, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Javdani Sepehri, Kyle Gleason
  • Patent number: 11275021
    Abstract: A substance testing method, a substance testing system, and a sensor module for a portable substance testing system is disclosed. The sensor module includes: a light source, configured to illuminate a lateral flow test strip of the portable substance testing system; a filter, comprising a plurality of apertures, configured to filter out light from the lateral flow test strip; and a sensor, for sensing the filtered light, and providing one or more signals indicative of an output of the test strip.
    Type: Grant
    Filed: September 1, 2017
    Date of Patent: March 15, 2022
    Assignee: Alcolizer Pty Ltd.
    Inventors: Roger Alan Hunt, James John Brown
  • Patent number: 11268953
    Abstract: Provided is a reagent composition for measuring glycated hemoglobin to diagnose the presence or absence of diabetes and a method of measuring glycated hemoglobin using the same, and more particularly is a reagent composition for measuring glycated hemoglobin, the composition including a dye-encapsulated silica nanoparticle-boronic acid, and to a method of measuring glycated hemoglobin using the same. In the reagent composition for measuring the glycated hemoglobin, since a dye is encapsulated in silica nanoparticles, the inherent absorption wavelength of the dye is not affected by pH and the composition has excellent stability even when stored for one month or more.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: March 8, 2022
    Assignee: DXGEN CORP.
    Inventors: Jin Woo Lee, Seon Ah Cheon
  • Patent number: 11199529
    Abstract: Contamination detection systems, kits, and techniques are described for testing surfaces for the presence of hazardous contaminants, while minimizing user exposure to these contaminants. Even trace amounts of contaminants can be detected. A collection kit provides a swab that is simple to use, easy to hold and grip, allows the user to swab large areas of a surface, and keeps the user's hands away from the surface being tested. The kit also provides open and closed fluid transfer mechanism to transfer the collected fluid to a detection device while minimizing user exposure to hazardous contaminants in the collected fluid. Contamination detection kits can rapidly collect and detect hazardous drugs, including trace amounts of antineoplastic agents, in healthcare settings at the site of contamination.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: December 14, 2021
    Assignee: Becton, Dickinson and Company
    Inventors: Weston F. Harding, Erik K. Witt, Austin Jason Mckinnon, Ray Isaacson, Bart Peterson, Marcel Arantes Souza, Matthew Oshinski
  • Patent number: 11007521
    Abstract: The invention relates to a discoid sample holder (1), on which a device (2) for carrying out at least one processing step is formed. According to the invention, a slot (3), into which a sampling instrument (4) can be introduced, and means (5) for releasing a sample from the sampling instrument (4) arranged in the receptacle (3), are formed in the sample holder.
    Type: Grant
    Filed: December 23, 2015
    Date of Patent: May 18, 2021
    Assignee: TESTO SE & CO. KGAA
    Inventors: Mike Muhl, Simon Brugger, Martin Zubler
  • Patent number: 10913068
    Abstract: A testing device is provided. The testing device includes a capturing tool and a microfluidic chip having a plurality of chambers connected in a network, a sample receiving port connected to the network, and a guide structure configured to receive the capturing tool, wherein the capturing tool is configured to capture sample in a distal position from the guide structure and further configured to transfer the captured sample to the sample receiving port in a proximal position from the guide structure.
    Type: Grant
    Filed: March 14, 2016
    Date of Patent: February 9, 2021
    Assignees: Nanyang Technological University, Agency For Science, Technology and Research, The Methodist Hospital
    Inventors: Joseph Sylvester Chang, Geok Soon Lim, Lei Zhang, Zhiping Wang, Ruige Wu, Stephen T. C. Wong, Kemi Cui
  • Patent number: 10832895
    Abstract: Provided is an analytical device including: a self-flowing microfluidic system, having a sample extraction location, at least one sample preparation location, and at least one sample analytical chamber; wherein the sample extraction location, the sample preparation location, and the at least one sample analytical chamber are interconnected by at least one microfluidic channel on a first substrate; and a signal readout system, having at least one sample analysis elements, and a data gathering and processing element.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: November 10, 2020
    Assignee: Plasmotica, LLC
    Inventors: Nedal Saleh, Waqas Khalid, Faisal Saleh
  • Patent number: 10788421
    Abstract: The present invention related to an electronic assay apparatus and a testing method thereof. The electronic assay apparatus for determining a result of an assay performed using a test strip comprises three light sources, only one detector and a microprocessor. The three light sources respectively illuminate light incident upon three different zones of the test strip. The detector detects light from the three zones alternately and the distances between the only one detector and the three light sources respectively are almost the same. Further, multiple openings corresponding to the three light sources respectively have the same size and/or shape. The microprocessor compares a calculating result value to only one threshold for showing a result.
    Type: Grant
    Filed: November 19, 2017
    Date of Patent: September 29, 2020
    Assignee: TAIDOC TECHNOLOGY CORPORATION
    Inventors: Chia-Chi Wu, Tien-Jung Tsai
  • Patent number: 10722883
    Abstract: A splittable fluid sample collector for dividing a fluid sample into two or more portions, includes at least first and second fluid collector portions detachably connected to one another, each of the fluid collector portions including a handle and an absorbent sample pad affixed to an end of the handle. Further, a fluid sampling apparatus includes the aforementioned splittable fluid sample collector, at least one container and a closure for the container. Still further, a method for splitting a fluid sample including providing the aforementioned splittable fluid sample collector, collecting a fluid sample with the absorbent sample pads of the splittable fluid sample collector and splitting the first and second fluid collector portions of the splittable fluid sample collector from one another.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: July 28, 2020
    Inventor: Moishe Bodner
  • Patent number: 10682829
    Abstract: Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: June 16, 2020
    Assignee: ILLUMINA, INC.
    Inventors: M. Shane Bowen, Bala Murali Venkatesan, Steven M. Barnard
  • Patent number: 10539580
    Abstract: Compositions and methods for detecting the presence and/or amount of one or more analytes, including analytes such as drugs of abuse, are provided. The compositions include two or more analytes associated with a solid phase, e.g., a particle or a multiwell plate. The compositions and methods also allow the simultaneous, tandem, or serial determination of the presence and/or amount of two or more analytes of interest in a sample.
    Type: Grant
    Filed: April 29, 2009
    Date of Patent: January 21, 2020
    Assignee: Psychemedics Corporation
    Inventors: Virginia Hill, Mohammad Atefi, Michael I. Schaffer
  • Patent number: 10436680
    Abstract: A cell capture, disruption, and extraction apparatus includes a disruption chamber configured to receive cell solution and having therein a plurality of abrasives, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The apparatus includes an actuation device configured to agitate the disruption chamber and/or pestle, movement of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. The pestle can rotate with respect to the disruption chamber. The pestle can include a nut-shaped core or axle, and/or include a plurality of extrusions. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content through the foregoing agitation process.
    Type: Grant
    Filed: October 11, 2014
    Date of Patent: October 8, 2019
    Inventor: Kianoosh Peyvan
  • Patent number: 10436781
    Abstract: A specimen delivery cartridge includes a lower housing, and an upper housing. The upper housing is coupled to the lower housing at a hinge. The specimen delivery cartridge further comprises a testing chamber comprising a paper testing substrate. The paper testing substrate may include a wicking conduit and a plurality of test areas. The specimen delivery cartridge may also include a lens assembly proximate the plurality of test areas and operable to transmit light emissions from the plurality of test areas to an image sensor of a computing device. In some embodiments, the specimen delivery cartridge includes a testing substrate having a plurality of test areas made of an array of electrodes. Each electrode is printed on a first side of the testing substrate and coupled to a conductive via formed in the testing substrate and a conductive trace printed on a second, opposing side of the testing substrate.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: October 8, 2019
    Assignee: PARATUS DIAGNOSTICS, LLC
    Inventors: John Carrano, Roland Schneider, John Jacob Carrano
  • Patent number: 10426403
    Abstract: A method of testing for specificity and strength of an allergic reaction is performed by penetrating the skin of an individual with the microneedle array comprising a plurality of epitopes and determining the response of the skin to the allergens. The response can be measured with a thermal imaging device. Analysis, and optionally treatment, can be provided to the individual.
    Type: Grant
    Filed: May 8, 2014
    Date of Patent: October 1, 2019
    Assignee: The Board of Trustees of The Leland Stanford Junior University
    Inventors: Manish J. Butte, Jayakumar Rajadas
  • Patent number: 10420337
    Abstract: An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue with a perfusate; and a sensor operatively connected to the perfusion circuit. The sensor includes a solid support to which is attached a recognition molecule that permits detection of the target agent. The recognition molecule specifically binds to the target agent in the presence of the target agent but not significantly to other agents. The sensor also includes an enzyme that can catalyze the conversion of a substance to glucose. In the presence of the target agent the enzyme can convert the substance into glucose, which can then be detected and optionally measured by the sensor.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: September 24, 2019
    Assignee: LIFELINE SCIENTIFIC, INC.
    Inventors: Steven L. Mayer, David C. Kravitz, Tracey H. Mayer
  • Patent number: 10412366
    Abstract: The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques, including imaging in three dimensions. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In some cases, the position of the entities can be determined in all three spatial dimensions (i.e., in the x, y, and z directions), and in certain cases, the positions in all three dimensions can be determined to an accuracy of less than about 1000 nm. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: September 10, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaowei Zhuang, Bo Huang, Wilfred M. Bates, Wenqin Wang
  • Patent number: 10345314
    Abstract: The present invention relates to a lateral flow membrane arrangement (1), comprising a microporous membrane layer (2) and a liquid-impermeable support layer (3), for lateral flow of a liquid through the microporous membrane layer (2), wherein the microporous membrane layer (2) is supported on the liquid-impermeable support layer (3) and has at least one detection zone (5) and at least one non-detection zone, wherein binding agents are immobilized in the at least one detection zone (5), the liquid-impermeable support layer (3) has at least one zone having a large thickness and at least one zone having a small thickness, the microporous membrane layer (2) is supported on the liquid-impermeable support layer (3) such that said at least one detection zone is provided above said at least one zone of the support layer having a large thickness and said at least one non-detection zone is provided above said at least one zone of the support layer having a small thickness, the zones are oriented in a direction orthogon
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: July 9, 2019
    Assignee: SARTORIUS STEDIM BIOTECH GMBH
    Inventors: Dieter Melzner, Denise Van Rossum
  • Patent number: 10274489
    Abstract: Assessing test results development during assay analysis is shown and described. In one embodiment, an apparatus generates an assessment of diagnostic test development from an assay. The apparatus may include an optical detector aligned in an optical path with the assay. Further, the optical detector may perform continuous image detection of the assay to generate an assessment testing development. In some examples, the assessment is a graphical display of diagnostic development. In yet other embodiments, a method of assessing diagnostic test development may include signaling the optical detector to perform continuing image detection of the assay and generating an assessment of said diagnostic test development. The assessment may include graphing a reflectance detected on the assay on a display board.
    Type: Grant
    Filed: February 11, 2014
    Date of Patent: April 30, 2019
    Assignee: Charm Sciences, Inc.
    Inventors: Robert J. Markovsky, Richard T. Skiffington, Stanley E. Charm
  • Patent number: 10258984
    Abstract: The invention relates to a cartridge (10) for processing of a liquid sample, for example for the detection of components in a sample of blood. The cartridge comprises a fluidic system with an intake port (12) leading via an intake capillary channel (13) to a storage chamber (14). Moreover, a feeding capillary channel (15) leads from the storage chamber (14) to a detection chamber (16). The design of the cartridge (10) is such that the intake capillary channel (13) that connects the intake port (12) to the storage chamber (14), has a capillary suction pressure sufficiently high to drive some sample from the intake port to the storage chamber without need of any additional pressure. Furthermore the cartridge a flow control element (18, 20) adapted to be externally controllable such that the sample can be drawn from the storage chamber (14) towards the processing chamber (16) without any active pumping.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: April 16, 2019
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Godefridus Johannes Verhoeckx, Toon Hendrik Evers, Marlieke Joan Overdijk, Monica Scholten, Nicole Henrica Maria Smulders, Joost Hubert Maas, Bernardus Jozef Maria Beerling
  • Patent number: 10254248
    Abstract: A unique fiber core sampler composition, related systems, and techniques for designing, making, and using the same are described. The sampler is used to interface with existing field instrumentation, such as Ion Mobility Spectrometer (IMS) equipment. Desired sampler characteristics include its: stiffness/flexibility; thermal mass and conductivity; specific heat; trace substance collection/release dependability, sensitivity and repeatability; thickness; reusability; durability; stability for thermal cleaning; and the like. In one form the sampler has a glass fiber core with a thickness less than 0.3 millimeter that is coated with a polymer including one or more of: polymeric organofluorine, polyimide, polyamide, PolyBenzlmidazole (PBI), PolyDiMethylSiloxane (PDMS), sulfonated tetrafluoroethylene (PFSA) and Poly(2,6-diphenyl-p-phenylene Oxide) (PPPO).
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: April 9, 2019
    Assignee: Battelle Memorial Institute
    Inventors: Raymond S Addleman, Xiaohong Shari Li, Wilaiwan Chouyyok, David A Atkinson
  • Patent number: 10247728
    Abstract: Embodiments described herein include detecting an analyte in a low pH sample. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: April 2, 2019
    Assignee: Charm Sciences, Inc.
    Inventors: Robert J. Markovsky, David W. Douglas
  • Patent number: 10207266
    Abstract: An microfluidic device includes a substrate (1) having a wandering groove (12) formed on a first surface (11) thereof; a shielding member (2) abutting on the first surface and covering the groove to form a flow channel (101) to deliver blood; an inlet (14) at one end of the flow channel for introducing the blood into the flow channel; and an outlet (16) at another end of the flow channel for discharging the blood. The flow channel (101) has a detecting region (102) for the blood passing through separately, the detecting region includes an entry (1021) for a single cell entering into at a time and an exit (1022) for a single cell exiting out at a time. A pair of electrodes (211) are disposed between the entry (1021) and the exit (1022) to detect the cells (200) of the blood.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: February 19, 2019
    Assignee: FOXCONN INTERCONNECT TECHNOLOGY LIMITED
    Inventor: Genn-Sheng Lee
  • Patent number: 10184946
    Abstract: A method for the integrated and automated analysis of DNA or protein, including a single-use cartridge, with an analysis device having a control device, and devices for capturing and processing signals, the control device carrying out a completely automatic process and evaluation of molecular diagnostic analysis via single-use cartridges (Lab-on-a-Chip). Controlling of an analysis process, which occurs in the cartridge, involve the subsequent displacement and thermostatisation of liquids with a first device, and with a second device the signals which are obtained during the analysis are processed. The first and the second devices are synchronized in such a manner that the analysis process of the sample can be carried out in a totally integrated manner thus producing an immediate result.
    Type: Grant
    Filed: August 18, 2015
    Date of Patent: January 22, 2019
    Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBH
    Inventors: Walter Gumbrecht, Peter Paulicka
  • Patent number: 10168329
    Abstract: Methods, compositions and kits for detecting Group A streptococcus in a biological sample are described. More particularly, the present disclosure provides an immunoassay in which the specificity of detection of Group A streptococcus is enhanced by addition of N-acetyl-D-glucosamine. These methods, compositions and kits are useful in convenient, reliable and early diagnosis of streptococcal infection in a human subject.
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: January 1, 2019
    Assignee: Quidel Corporation
    Inventors: Peter Yan-Guo Ren, Jason McClure, Kevin S. Richardson
  • Patent number: 10156568
    Abstract: Techniques for detection of virus-antibody nanocomplexes using a chip-based pillar array are provided. In one aspect, a method for virus detection is provided. The method includes the steps of: collecting a fluid sample from a virus-bearing source; contacting the fluid sample with an antibody that binds to viruses to form a sample-antibody mixture, wherein the antibody is labeled with a fluorescent tag; separating particles including any antibody-virus complexes, if present, from the sample-antibody mixture using an assay nanopillar array; and detecting the antibody-virus complexes, if present, in the particles from the separating step using fluorescence. A virus detection chip device and a chip-based immunoassay method are also provided.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: December 18, 2018
    Assignee: International Business Machines Corporation
    Inventors: Yann Astier, Stacey M. Gifford, Benjamin H. Wunsch
  • Patent number: 10145841
    Abstract: The present invention provides a method for detecting an antigen in an analysis sample, the method including: (a) contacting an analysis sample with a detection antibody with which a marker generating a detectable signal is combined and which specifically binds to the antigen; (b) contacting a capture antibody with the resultant product of step (a), the capture antibody specifically binding to an antigen to be detected; (c) contacting the detection antibody, with which the marker generating a detectable signal is combined, with a reference substance which is bound to a surface of a solid substrate and which includes an epitope to which the detection antibody specifically binds; (d) measuring signals generated from the markers of the resultant product of step (b) and the resultant product of step (c); and (e) analyzing the measured signals to determine the presence or absence and amount of the antigen in the analysis sample.
    Type: Grant
    Filed: February 20, 2013
    Date of Patent: December 4, 2018
    Assignee: NANOENTEK, INC.
    Inventors: Chan Il Chung, Chang Seop Lee, Jeoung Ku Hwang, Jong Sik Jeong