Carrier Is Particulate And The Particles Are Of Intentionally Different Sizes Or Impregnated Differently With The Immunochemicals Patents (Class 436/523)
  • Patent number: 10309918
    Abstract: The present application relates to a sensing method that is carried out using an electrode that comprises an electrode substrate functionalized with sensing elements. The method involves conducting electrochemical impedance spectroscopy at a plurality of applied voltages and then integrating measurement data as a function of voltage. Also provided is an apparatus for carrying out the sensing method. The method and apparatus are suitable for a broad range of sensing applications, including the detection of diagnostic biomarkers, drug screening, development of glycoarray systems and the sensing of environmental parameters such as light intensity, temperature and humidity.
    Type: Grant
    Filed: July 6, 2017
    Date of Patent: June 4, 2019
    Assignees: Oxford University Innovation Limited, Universidade Estadual Paulista “Julio de Mesquita-Filho”—UNESP
    Inventors: Jason Davis, Paulo Roberto Bueno
  • Patent number: 10107804
    Abstract: A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: October 23, 2018
    Assignee: TRUSTEES OF TUFTS COLLEGE
    Inventors: David R. Walt, Karri Lynn Michael-Ballard
  • Patent number: 9927328
    Abstract: The present invention provides a method of separating beads in a fluidic chip comprising an internal fluid circuit through which various reactants, in which at least one of the reactants are beads, may be moved by use of centrifugal force, the method comprises the steps of: providing at least a first set of beads (8a) having a density m1 and a second set of beads (8b) having a density m2 in a section (7, 15, 18) of the fluid circuit, the section comprising at least a first outlet (16, 13, 17); providing a first liquid medium in the section, the liquid medium having a density d3, such that m1<d3<m2; and applying a first centrifugal force (G) such that the first set of beads (8a) and the second set of beads (8b) migrates in opposite directions within the section.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: March 27, 2018
    Assignee: SpinChip Diagnostics AS
    Inventors: Stig Morten Borch, Torgeir Hamsund, Jostein Geir Holtlund, Anja Gulliksen
  • Patent number: 9568488
    Abstract: In a biological sample analyzing apparatus and method, an assay sample is prepared by mixing a reagent with a biological sample which may contain assay material. Then, a first information relating to the assay material is collected from the assay sample, and when the first information satisfies predetermined condition, the assay material is analyzed based on the first information. However, when the first information does not satisfy the predetermined condition, a second information related to the assay material is collected from the assay sample, and the assay material is analyzed based on the second information.
    Type: Grant
    Filed: October 21, 2005
    Date of Patent: February 14, 2017
    Assignee: SYSMEX CORPORATION
    Inventors: Teruya Matsumoto, Yukie Nakazawa
  • Patent number: 9518986
    Abstract: An aptamer-based SERS detection technique that directly monitors an aptamer-analyte capture event by generating spectroscopic information regarding the identity of the analyte that has been bound to the aptamer from a complex biological sample. A reproducible SERS spectrum is measured for an aptamer-analyte complex formed on a metal surface and this spectral information is used directly to identify the specific aptamer-analyte complex and optionally also to quantify the analyte in the sample, thus enabling discrimination between true and false positives in quantitative analyte assays on complex biological samples. In one embodiment the aptamer is attached directly to the metal surface and surrounded by a self-assembled monolayer (SAM) of amphiphilic molecules. In an alternative embodiment the metal surface is coated with a SAM and the aptamer is attached to the amphiphilic molecules of the SAM.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: December 13, 2016
    Assignee: University of Cape Town
    Inventors: Jonathan Michael Blackburn, Michael Evans, Sriram Krishnan, Christa Lynn Brosseau
  • Patent number: 9297766
    Abstract: Surface-enhanced Raman spectroscopy (SERS) uses nanoscale metal particles (SERS-active particles) or surface roughness to enhance the Raman signal of Raman-active analytes contacting the surface. SERS sandwich particles contain SERS-active particles sandwiching a Raman-active substance and serve as optical tags. Preferably, the particles are rod-shaped, with each layer (SERS-active and Raman-active) formed as a distinct stripe of the particle. These freestanding particles can be derivatized with surface ligands capable of associating with analytes of interest in, for example, a biological sample. The acquired Raman spectrum of the particle encodes the identity of the ligand. Because of the simplicity and intensity of Raman spectra, highly multiplexed assays are capable using SERS particles with different Raman-active species.
    Type: Grant
    Filed: May 20, 2005
    Date of Patent: March 29, 2016
    Assignee: Becton, Dickinson and Company
    Inventor: Michael J. Natan
  • Patent number: 9201013
    Abstract: Metal nanoparticles associated with a spectroscopy-active (e.g., Raman-active) analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy (e.g., surface-enhanced Raman spectroscopy).
    Type: Grant
    Filed: May 18, 2005
    Date of Patent: December 1, 2015
    Assignee: Becton, Dickinson and Company
    Inventor: Michael J. Natan
  • Patent number: 9149440
    Abstract: This invention relates to polymeric nanoparticles useful for drug delivery with target molecules bonded to the surface of the particles and having sizes of up to 1000 nm, preferably 1 nm to 400 nm, more preferably 1 nm to 200 nm, that are dispersed homogeneously in aqueous solution. The target drug/target substance is covalently bonded to the novel polymeric nanoparticles to secure them from outer intervention in vivo or cell culture in vitro until they are exposed at the target site within the cell. This invention also relates to microemulsion polymerization techniques useful for preparing the novel nanoparticles.
    Type: Grant
    Filed: September 2, 2004
    Date of Patent: October 6, 2015
    Assignee: UNIVERSITY OF SOUTH FLORIDA
    Inventors: Edward Turos, Jeung-Yeop Shim
  • Patent number: 9046477
    Abstract: Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: June 2, 2015
    Assignee: Life Technologies Corporation
    Inventors: Stephen Empedocles, Andrew Watson, Jian Jin
  • Patent number: 9040310
    Abstract: Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.
    Type: Grant
    Filed: April 27, 2011
    Date of Patent: May 26, 2015
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Julia Ashworth-Sharpe, Chol Steven Yun, Zhanna Zhilina, Adrian E. Murillo, Donald D. Johnson, Michael Farrell, Jerome W. Kosmeder, Christopher Bieniarz
  • Patent number: 9005995
    Abstract: The present invention relates to a nanoscale or microscale particle for encapsulation and delivery of materials or substances, including, but not limited to, cells, drugs, tissue, gels and polymers contained within the particle, with subsequent release of the therapeutic materials in situ, methods of fabricating the particle by folding a 2D precursor into the 3D particle, and the use of the particle in in-vivo or in-vitro applications. The particle can be in any polyhedral shape and its surfaces can have either no perforations or nano/microscale perforations. The particle is coated with a biocompatible metal, e g gold, or polymer e g parvlene, layer and the surfaces and hinges of the particle are made of any metal or polymer combinations.
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: April 14, 2015
    Assignee: The Johns Hopkins University
    Inventors: David H. Gracias, Timothy Gar-Ming Leong, Hongke Ye
  • Patent number: 9005994
    Abstract: The present application pertains to improved methods of detecting biomolecules in a biological sample (or system), In particular, embodiments discussed herein allow for the detection of biomolecule complexes. The embodiments enable for the first time the elucidation of the significance of biomolecule complexes for certain disease states, which in turn enables the diagnosis of disease states based on the identity and complexing level of a biomolecule complex in a particular biological sample.
    Type: Grant
    Filed: January 12, 2011
    Date of Patent: April 14, 2015
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventor: Qun Huo
  • Patent number: 8993349
    Abstract: Semiconductor nanoparticle complexes comprising semiconductor nanoparticles in association with cationic polymers are described. Also described are methods for enhancing the transport of semiconductor nanoparticles across biological membranes to provide encoded cells. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. Kits comprising reagents for performing such methods are also provided.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: March 31, 2015
    Assignee: Life Technologies Corporation
    Inventors: Marcel P. Bruchez, R. Hugh Daniels, Jennifer Dias, Larry C. Mattheakis, Hongjian Liu, Aquanette M. Burt, Berndt Christoffer Lagerholm, Danith H. Ly
  • Patent number: 8980572
    Abstract: A method for generating and localizing a signal in a solid phase substrate detection system comprises applying a solution of target material to a substrate; binding the target with a specific affinity molecule having an attached label, the label comprising multiple signal precursor molecules; applying a carrier to the substrate, and treating the label to convert the signal precursor molecules to signal generating molecules. The carrier comprises solvent for the label and thickener for localizing the signal. The carrier may include developer that converts signal precursor molecules to signal generating molecules. Developer is not necessary if the signal precursor molecules are converted to signal generating molecules by e.g. temperature change, pH change, sonication, light irradiation, microwave heating. A test device for detecting target in a fluid sample, and a kit of parts for determining the presence of target in a fluid sample are also disclosed.
    Type: Grant
    Filed: June 14, 2010
    Date of Patent: March 17, 2015
    Assignee: Supernova Diagnostics, Inc.
    Inventors: Ling Wai Wong, Pui Yee Cangel Chan, Wing Cheung Mak, King Keung Sin, Reinhard Renneberg
  • Patent number: 8969100
    Abstract: Regions where metastatic cancer cells can exist are detected with high accuracy in a sentinel lymph node. Quantum dots are injected into the vicinity of a cancer in a living body, thereby identifying the location of the sentinel lymph node by means of fluorescence. Subsequently, the sentinel lymph node is extracted. With respect to the sentinel lymph node extracted with quantum dots injected, structural analysis is conducted by means of precision fluorescence measurement which uses a confocal fluorescence microscope for monomolecular observation. Specifically, the fluorescence intensity is measured with respect to each of multiple areas in the sentinel lymph nodes, and out of the multiple areas measured, one or more areas are detected as afferent lymph vessel inflow regions in descending order of fluorescence intensity.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: March 3, 2015
    Assignees: Tohoku University, Konica Minolta Medical & Graphic, Inc.
    Inventors: Makoto Hikage, Kohsuke Gonda, Motohiro Takeda, Takashi Kamei, Noriaki Ohuchi, Hideki Gouda, Yasushi Nakano
  • Patent number: 8962345
    Abstract: A method of characterizing glycans attached to glycoproteins is disclosed herein. The method comprises a first step of immobilizing the glycoproteins on colloidal particles forming glycoprotein/colloidal particles. The glycans on the glycoproteins may then be characterized, for example the composition and/or structure of glycans may be characterized or the glycans attached to proteins may be identified. Characterization may be accomplished by either binding the glycoprotein/colloidal particles with one or more binding agents and assessing the aggregation of the glycoprotein/colloidal particles or by cleaving glycans from the glycoprotein/colloidal particles with a cleaving agent and analyzing the glycans.
    Type: Grant
    Filed: May 20, 2011
    Date of Patent: February 24, 2015
    Assignees: The United States of America as represented by the Secretary of Commerce, The National Institute of Standards and Technology
    Inventors: Rebecca A. Zangmeister, Germarie Sanchez-Pomales, Todd A. Morris, Michael J. Tarlov
  • Patent number: 8962344
    Abstract: A membrane-coated particle composition and methods comprising a particle surrounded by a native cell membrane are disclosed. The cell membrane may contain selected receptors or binding components. At least a portion of the receptors or binding components are oriented on the membrane-coated particle in the same or similar orientation as in the native cell membrane. The membrane-coated particle(s) finds use, for example, in contexts of basic research, proteomics, drug discovery, drug delivery, medical diagnostics, and aspects of patient care.
    Type: Grant
    Filed: March 16, 2009
    Date of Patent: February 24, 2015
    Assignee: Synamem Corporation
    Inventors: John T. Groves, Jeremy T. Blitzer
  • Patent number: 8951474
    Abstract: The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.
    Type: Grant
    Filed: February 5, 2010
    Date of Patent: February 10, 2015
    Assignee: On-Chip Biotechnologies Co., Ltd.
    Inventor: Kazuo Takeda
  • Patent number: 8951743
    Abstract: The present invention stems from the finding that the extracellular domain of CD31 proteins present on blood leukocytes is shed and released in the circulation as a soluble form of CD31. A method for detecting shed CD31 is further disclosed. The invention therefore relates to a method for detecting a shed ectodomain of a transmembrane protein such as CD31 and to the use of such a method as a diagnostic tool. The invention further provides methods for determining whether a candidate protein is part of a molecular complex.
    Type: Grant
    Filed: June 30, 2009
    Date of Patent: February 10, 2015
    Assignee: Institut National de la Sante et de la Recherche Medicale (INSERM)
    Inventors: Giuseppina Caligiuri, Antonino Nicoletti
  • Patent number: 8932485
    Abstract: The present invention provides fluorescent nanoparticle composites themselves, the process of preparing such composites, to systems for rapid diagnosis (as “kits”) containing such composites, and to the use of such composites. In a preferential embodiment, the composites of the present invention have an affinity for biological molecules, such as DNA. The present invention also comprises the preparation of probes containing biological material, upon which are added fluorescent nanoparticle composites, making viable a rapid and economic biological diagnosis of, for example, diseases and genetic traits, notably in the medical and veterinarian fields.
    Type: Grant
    Filed: March 23, 2009
    Date of Patent: January 13, 2015
    Assignee: Universidade Federal de Pernambuco-UFPE
    Inventors: Celso Pinto De Melo, César Augusto Souza De Andrade, Clécio Gomes Dos Santos
  • Patent number: 8932877
    Abstract: Disclosed are photoluminescent particles. The particles include a core nano-sized particle of carbon and a passivation agent bound to the surface of the nanoparticle. The passivation agent can be, for instance, a polymeric material. The passivation agent can also be derivatized for particular applications. For example, the photoluminescent carbon nanoparticles can be derivatized to recognize and bind to a target material, for instance a biologically active material, a pollutant, or a surface receptor on a tissue or cell surface, such as in a tagging or staining protocol.
    Type: Grant
    Filed: September 28, 2010
    Date of Patent: January 13, 2015
    Assignee: Clemson University Research Foundation
    Inventor: Ya-Ping Sun
  • Patent number: 8927290
    Abstract: Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.
    Type: Grant
    Filed: February 16, 2011
    Date of Patent: January 6, 2015
    Assignee: One Lambda, Inc.
    Inventors: Adam Idica, Chun-Tsan Deng, Paul I. Terasaki
  • Patent number: 8900882
    Abstract: In the case of assaying the level of complex AB in a sample which likely contains the complex AB composed of substance A with another substance B, the complex is assayed by the competitive homogeneous assay method with the use of reagents including a reagent containing partner C specifically binding to substance A, a reagent containing partner D specifically binding to substance B, a reagent containing fine particles carrying substance A or an analog thereof and substance B or an analog thereof, and a reagent containing partner C specifically binding to substance A and partner D specifically binding to substance B. Thus, the complex in the sample can be easily assayed. The above method is applicable to general-purpose biochemical automatic analyzers.
    Type: Grant
    Filed: May 14, 2009
    Date of Patent: December 2, 2014
    Assignees: Nitto Boseki Co., Ltd., Shinshu University
    Inventors: Kiyotaka Fujita, Ryo Kojima, Yoshiro Sato, Natsuki Sato
  • Patent number: 8865477
    Abstract: A novel quantum dot capable of near infrared emissions at wavelengths of 750-1100 is made by forming solid solutions of metal sulfide, metal selenide or metal sulfide selenide by incorporating a suitable amount of an additional metallic element or elements to provide an emission wavelength in the range of 750 nm to 1100 nm. The quantum dots may be enabled for bioconjugation and may be used in a method for tissue imaging and analyte detection.
    Type: Grant
    Filed: April 22, 2009
    Date of Patent: October 21, 2014
    Assignee: Drexel University
    Inventors: Wei-Heng Shih, Wan Y. Shih, Hui Li, Ian McDonald, Andrew Kopek, Ryan O'Malley, Yu-Chieh Lu
  • Patent number: 8865476
    Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: October 21, 2014
    Assignee: Life Technologies Corporation
    Inventors: Michael D. Ward, Carleton C. Stewart, Gregory Kaduchak
  • Patent number: 8859297
    Abstract: The disclosure relates to the detection of analytes (e.g., biological pathogens such as bacteria or viruses) using a conductive polymer label. The disclosed detection system utilizing the conductive polymer label generally involves the formation of an analyte conjugate between the target analyte and a conductive polymer moiety conjugated to the target analyte. The conductive polymer portion of the analyte conjugate is electrically activated to form an electrically activated analyte conjugate having an increased electrical conductivity relative to the analyte conjugate as originally formed. The electrically activated analyte conjugate can then be detected by any suitable means, such as by conductimetric or electrochemical detection.
    Type: Grant
    Filed: May 22, 2012
    Date of Patent: October 14, 2014
    Assignee: Board of Trustees of Michigan State University
    Inventors: Evangelyn C. Alocilja, Emma Setterington
  • Patent number: 8835186
    Abstract: The present invention relates to methods, compositions and kits for affinity isolation, affinity purification and affinity assay based on microbubbles coated with an affinity molecule. Particularly, the invention provides protein microbubbles coated with an affinity molecule. In addition, the invention provides glass microbubbles coated with an affinity molecule. Methods of using the microbubbles of the invention for isolating analytes and cells are specifically provided.
    Type: Grant
    Filed: November 3, 2005
    Date of Patent: September 16, 2014
    Assignee: Iris International, Inc.
    Inventors: Edward Jablonski, Thomas Adams
  • Patent number: 8802446
    Abstract: Provided is a method and reagent for measuring cystatin C in a human body fluid, by contacting a human body fluid with (a) first insoluble carrier particles coated with a first anti-human cystatin C monoclonal antibody and (b) second insoluble carrier particles coated with a second anti-human cystatin C monoclonal antibody, where the first anti-human cystatin C monoclonal antibody has an affinity for cystatin C that is higher than the second anti-human cystatin C monoclonal antibody, the second anti-human cystatin C monoclonal antibody recognizes an epitope of cystatin C that is different from the epitope recognized by the first anti-human cystatin C monoclonal antibody, and the first and second insoluble carrier particles are coated with from 1% to less than 4% by weight of the anti-human cystatin C monoclonal antibodies.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: August 12, 2014
    Assignee: Sekisui Medical Co., Ltd.
    Inventors: Shinya Nakayama, Hiroshi Takahashi, Yasushi Nakamura, Tomo Shimizu
  • Patent number: 8796040
    Abstract: A system of quantitatively determining a biomolecule, which has: allowing fluorescent silica particles capable of emitting fluorescence detectable by a flow cytometer to capture a target biomolecule fluorescent-labelled for quantitative determination; detecting the fluorescence emitted from the fluorescent silica particles themselves by using the flow cytometer; and measuring the intensity of the fluorescence of the labelled target biomolecule, thereby quantitatively determining the target biomolecule.
    Type: Grant
    Filed: August 22, 2008
    Date of Patent: August 5, 2014
    Assignees: The Furukawa Electric Co., Ltd., The University of Tokushima
    Inventors: Hideki Aizawa, Michio Ohkubo, Michihiro Nakamura, Hirokazu Miyoshi
  • Patent number: 8771972
    Abstract: Novel conjugates and immunogens derived from clozapine and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of clozapine in biological fluids.
    Type: Grant
    Filed: July 19, 2011
    Date of Patent: July 8, 2014
    Assignee: Saladax Biomedical Inc.
    Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Howard Sard, Christopher Spedaliere
  • Patent number: 8771971
    Abstract: The present invention provides simple and rapid methods for measuring the function of a desired subset of lymphocytes, for example, T cells, B cells or NK cells. In addition, the present invention provides an all-in-one kit that contains reagents which permit a rapid and reliable analysis of the functions of T cells, B cells and NK cells obtained directly from whole blood or cord blood.
    Type: Grant
    Filed: July 30, 2007
    Date of Patent: July 8, 2014
    Assignee: The Research Foundation of State University of New York
    Inventor: Allen J. Norin
  • Patent number: 8722419
    Abstract: Mass cytometry method. In one aspect, the method includes providing a sample having at least one cell type and mixing the sample with material such as nanoparticles functionalized with affinity molecules for the at least one cell type. The sample is transported through a suspended microchannel resonator to record a mass histogram and a cell count for the at least one cell type is determined from the histogram.
    Type: Grant
    Filed: June 15, 2007
    Date of Patent: May 13, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Scott R. Manalis, Thomas P. Burg, Michel Godin, Kenneth Babcock
  • Patent number: 8717045
    Abstract: A microorganism number-measuring apparatus includes: measurement container including measurement liquid; rotary driver; bacteria-collection signal generator; measurement signal generator; output amplifier for amplifying outputs of signal generators and; I/V amplifier; impedance measuring unit for measuring impedance of liquid; microorganism number-computing unit for computing the number of microorganisms present in liquid; solution conductivity-computing unit for computing conductivity of liquid; and warm-up section for warming up at least one of I/V amplifier and output amplifier. Warm-up section computes a warm-up signal based on the conductivity computed by conductivity-computing unit. Warm-up section computes the warm-up signal having a current the same in magnitude as that flowing through measurement electrode by using the measured solution conductivity, and applies the signal to at least one of I/V amplifier and output amplifier.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: May 6, 2014
    Assignee: Panasonic Healthcare Co., Ltd.
    Inventor: Hidenori Morita
  • Patent number: 8715951
    Abstract: Disclosed are antibodies that selectively bind to blood coagulation factor FVIII, and highly sensitive immunological assays comprising these antibodies. Preferred assays can detect FVIII at about 3500-fold below the normal physiological levels, and have a wide array of applications including accurate monitoring of FVIII concentration in pharmaceutical products for treatment of blood coagulation disorders, and determination of FVIII levels in plasma of human patients, including those with blood coagulation disorders such as hemophilia.
    Type: Grant
    Filed: July 18, 2012
    Date of Patent: May 6, 2014
    Assignee: The University of Vermont and State Agriculture College
    Inventors: Behnaz Parhami-Seren, Kenneth G. Mann, David N. Fass
  • Patent number: 8709829
    Abstract: The present invention relates to a nanoscale or microscale particle for encapsulation and delivery of materials or substances, including, but not limited to, cells, drugs, tissue, gels and polymers contained within the particle, with subsequent release of the therapeutic materials in situ, methods of fabricating the particle by folding a 2D precursor into the 3D particle, and the use of the particle in in-vivo or in-vitro applications The particle can be in any polyhedral shape and its surfaces can have either no perforations or nano/microscale perforations The particle is coated with a biocompatible metal, e g gold, or polymer e g parvlene, layer and the surfaces and hinges of the particle are made of any metal or polymer combinations.
    Type: Grant
    Filed: August 20, 2012
    Date of Patent: April 29, 2014
    Assignee: The Johns Hopkins University
    Inventors: David H. Gracias, Timothy Gar-Ming Leong, Hongke Ye
  • Patent number: 8691591
    Abstract: Disclosed herein are compositions and methods for combining the output obtained from redundant sensor elements in a sensor array.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: April 8, 2014
    Assignee: Trustees of Tufts College
    Inventors: David R. Walt, Todd A. Dickinson
  • Patent number: 8685753
    Abstract: Methods are provided for detecting and optionally quantitating multiple analytes, including nucleic acid and/or polypeptide analytes, in particle-based assays that can be highly multiplexed. Compositions, systems, and kits related to the methods are also featured.
    Type: Grant
    Filed: January 10, 2012
    Date of Patent: April 1, 2014
    Assignee: Affymetrix, Inc.
    Inventors: Jason Martin, Quan Ngoc Nguyen
  • Patent number: 8658432
    Abstract: An objective is to provide Si/Si3N4 system nanosized particles each exhibiting reduced environmental load together with minimized bio toxicity, excellent chemical stability, enhanced relative light emission intensity, and less degradation of emission intensity during continuous exposure to light; a biosubstance labeling agent capable of keeping on labeling a biosubstance exhibiting luminance enhanced for a long duration with the Si/Si3N4 system nanosized semiconductor particle of the present invention; and also a method of manufacturing the Si/Si3N4 system nanosized semiconductor particle of the present invention.
    Type: Grant
    Filed: January 23, 2007
    Date of Patent: February 25, 2014
    Assignee: Konica Minolta Medical & Graphic, Inc.
    Inventors: Naoko Furusawa, Yasushi Nakano, Kazuya Tsukada
  • Patent number: 8628932
    Abstract: The invention is directed to methods of screening for HLA antibodies comprising detecting antibodies specific for native HLA antigens and denatured HLA antigens. The invention also provides for methods of removing antibodies specific for denatured HLA antigens or antibodies specific for native HLA antigens from a serum sample. In addition, the invention also provides for method of predicting whether a transplant recipient has an increased risk for rejecting the transplanted organ.
    Type: Grant
    Filed: March 9, 2011
    Date of Patent: January 14, 2014
    Assignee: One Lambda
    Inventors: Jar-How Lee, Rui Pei, Thoa Nong
  • Patent number: 8617824
    Abstract: A rapid and sensitive analyte detection assay is based on whispering gallery modes of fluorescently labelled microspheroidal particles. Ligands for the analyte, such as nucleic acids, are anchored to the particles. The fluorescent labels may comprise fluorophores or quantum dots. In the latter case, the particles may comprise melamine formaldehyde. The assay may be used to detect analytes in aqueous samples.
    Type: Grant
    Filed: November 20, 2009
    Date of Patent: December 31, 2013
    Assignees: Genera Biosystems Limited, The University of Melbourne
    Inventors: Karl Frederick Poetter, Edin Nuhiji, Paul Mulvaney
  • Patent number: 8603828
    Abstract: Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.
    Type: Grant
    Filed: November 8, 2010
    Date of Patent: December 10, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Roger Walker, Benedicte Jardin
  • Patent number: 8580530
    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies of an individual, comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes, erythrocyte membrane fragments or blood group antigens.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: November 12, 2013
    Assignee: Bio-Rad Innovations
    Inventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
  • Patent number: 8580531
    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: November 12, 2013
    Assignee: Bio-Rad Innovations
    Inventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
  • Patent number: 8569076
    Abstract: A method and system for performing a serological agglutination assay in a liquid sample. The system provides a simple method for creating an in-situ sample/reagent admixture within a sample analysis chamber without the use of any precision fluid-handling components.
    Type: Grant
    Filed: April 2, 2009
    Date of Patent: October 29, 2013
    Assignee: Abbott Point of Care, Inc.
    Inventors: Stephen C. Wardlaw, Robert A. Levine
  • Patent number: 8563330
    Abstract: The invention provides a device and method for the rapid identification of patients suspected of having thalassemia. The invention provides a test strip for the aqueous detection of thalassemia related proteins in whole blood. The test strip includes antibodies specific to the gamma 4, (?4) protein and provides easy visual discrimination between a positive result and a negative result. The invention can be used in remote or clinical settings.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: October 22, 2013
    Assignee: National Science and Technology Development Agency
    Inventors: Watchara Kasinrerk, Chatchai Tayapiwatana, Thanusak Tatu, Suthat Fucharoen, Sawitree Chiampanichayakul
  • Patent number: 8557531
    Abstract: Endothelial cells are detected in a blood sample by enriching the endothelial cells from the blood sample followed by performing on the enriched endothelial cells an immunoassay capable of detecting antigens expressed by the endothelial cells. The immunoassay is capable of detecting antigen expressed from 300 endothelial cells per milliliter of blood. The method can be used for assaying mature circulating endothelial cells or circulating endothelial progenitor cells.
    Type: Grant
    Filed: April 18, 2007
    Date of Patent: October 15, 2013
    Assignee: Wellstat Biologics Corporation
    Inventor: Robert M. Lorence
  • Patent number: 8557590
    Abstract: A method for measuring glycated hemoglobin includes hemolyzing a blood sample with a hemolysate; reacting the hemolyzed blood sample with bead conjugates in which beads are conjugated with glycated hemoglobin binding materials; measuring the amount of total hemoglobin in the reacted blood sample; isolating normal hemoglobin from the glycated hemoglobin conjugated with the bead conjugates; measuring the amount of glycated hemoglobin isolated from the normal hemoglobin; and determining the percentage of the glycated hemoglobin in the blood sample on the basis of the measured amounts of total hemoglobin and glycated hemoglobin. The isolation of normal hemoglobin is performed by absorbing the normal hemoglobin using an absorption pad that is a porous pad having pores, each of which has a size greater than the size of the normal hemoglobin and smaller than the size of the bead.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: October 15, 2013
    Assignee: Infopia Co., Ltd.
    Inventors: Byeong-Woo Bae, Sung-Dong Lee, Hyong-Soo Kim
  • Patent number: 8546095
    Abstract: The methods of the disclosure provide fluorescence-based assays for calcineurin activity, especially in isolated T cells. The methods include the stimulation of the T cells with agents that specifically target the TCR with or without influencing co-stimulatory pathways. One TCR agonist is monoclonal antibodies specific for CD3, which more precisely distinguish the inducible activity of calcineurin than does an alternative method targeting the T cell receptor (CD3) combined with CD28 costimulation. This method more accurately distinguishes between the measured level of calcineurin activity of T cells from immunosuppressed transplant recipients and normal individuals, and thus has improved diagnostic accuracy with respect to the response of an individual to immunosuppressant therapy following an organ transplant.
    Type: Grant
    Filed: January 20, 2010
    Date of Patent: October 1, 2013
    Assignee: Emory University
    Inventors: James Tumlin, Allan D. Kirk, Brian R. Roberts, Jennifer Gooch
  • Patent number: 8518710
    Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: August 27, 2013
    Assignee: Life Technologies Corporation
    Inventors: Tom Knapp, Gregory Zlokarnik, Paul Negulescu, Roger Tsien, Timothy Rink
  • Patent number: 8513032
    Abstract: The present invention relates to methods, compositions and kits for affinity isolation, affinity purification and affinity assay based on microbubbles coated with an affinity molecule. Particularly, the invention provides protein microbubbles coated with an affinity molecule. In addition, the invention provides glass microbubbles coated with an affinity molecule. Methods of using the microbubbles of the invention for isolating analytes and cells are specifically provided.
    Type: Grant
    Filed: May 31, 2011
    Date of Patent: August 20, 2013
    Assignee: Iris International, Inc.
    Inventors: Edward Jablonski, Thomas Adams