Involving An Insoluble Carrier For Immobilizing Immunochemicals Patents (Class 436/518)
  • Patent number: 10653017
    Abstract: The present disclosure provides a glass layer for a display screen and a display screen, including: a glass layer body having a transverse direction and a longitudinal direction perpendicular to each other; a plurality of ribs spaced apart from each other and arranged on an upper surface of the glass layer body, a groove being formed between every two adjacent said ribs; a movable brush arranged on an outer surface of each of said ribs; a drive connection mechanism connecting with the movable brush for driving the movable brush to be reciprocated on the outer surface of said ribs.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: May 12, 2020
    Assignee: BOE TECHNOLOGY GROUP CO., LTD.
    Inventor: Xiao Chu
  • Patent number: 10634602
    Abstract: The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: April 28, 2020
    Assignee: CYTOCHIP INC.
    Inventors: Wendian Shi, Yuzhe Ding
  • Patent number: 10555886
    Abstract: A composition of algal extracts containing phycobiliprotein is provided. This composition not only effectively inhibit the growth of oral pathogens including Porphyromonathes gingivalis and Actinobacillus actinomycetemcomitans, but also reduces the inflammation caused by those pathogens. Therefore, the phenomenon of alveolar bone loss in periodontal patients is mitigation, achieving the effect of prevention and treatment of periodontal disease.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: February 11, 2020
    Assignee: FAR EAST BIO-TEC CO., LTD.
    Inventors: Tz-Chong Chou, Chuang-Chun Chiuh
  • Patent number: 10539559
    Abstract: Disclosed are a PIVKA-II measurement method that achieves better serum-plasma correlation than conventional methods, and a reagent and a kit therefor. The PIVKA-II measurement method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using a mixture of an anti-F1 antibody that specifically binds to prothrombin fragment F1 or an antigen-binding fragment thereof and an anti-F2 antibody that specifically binds to prothrombin fragment F2 or an antigen-binding fragment thereof; and an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof.
    Type: Grant
    Filed: August 6, 2013
    Date of Patent: January 21, 2020
    Assignees: FUJIREBIO INC., SEKISUI MEDICAL CO., LTD.
    Inventors: Kentaro Yamaguchi, Katsumi Aoyagi, Azusa Terao
  • Patent number: 10518267
    Abstract: Embodiments of the invention provide a filter kit including filters for processing a biological sample. Some embodiments include a filter cap in a tube kit with a first tube containing a buffer solution and a second tube containing a lyophilized master mix. Some embodiments include a method of processing a sample using the kit including mixing a biological sample in a first tube with the buffer solution, positioning the filter cap in the first tube, positioning a second tube on the filter cap, flipping the first tube, the filter cap, and the second cap to filter the biological sample and buffer solution mixture with the filter cap as it flows from the first tube to the second tube. Some embodiments include structure enabling transfer of materials through inline flow between the tubes. Some further embodiments include integrated structure for sample pulverization with integrated buffer and lyophilized master mix.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: December 31, 2019
    Assignee: DOUGLAS SCIENTIFIC, LLC
    Inventors: Robert Hjelseth, Cory Hodgson, Andrew Bristow, Mark Baumgartner, David Sellnow, Cassie Keppel, John Steckelberg, Paul Newman
  • Patent number: 10495634
    Abstract: The present invention provides a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a liquid sample by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD4+, CD8+ cells, malaria-infected red blood cells, cancer cells, cancer biomarkers such as prostate specific antigen and other cancer biomarkers, viruses, bacteria, and other pathogenic agents, with the sensitivity, specificity and accuracy of bench-top laboratory-based assays.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: December 3, 2019
    Assignee: Carnegie Mellon University
    Inventors: Alberto Gandini, James F. Antaki, Byron Wang Chuan, Joie N. Marhefka
  • Patent number: 10472382
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable Media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: November 12, 2019
    Assignee: QIAGEN SCIENCES, LLC
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 10458990
    Abstract: A flow of air including a fungal spore is directed to a collection cartridge. The spore is trapped within the cartridge and positioned within a field of view of a camera sensor. A UV light is activated to illuminate the spore and a camera shutter is opened for a time period. The camera sensor collects light emitted from the spore during a first portion of the time period. After the first portion has elapsed, first and second bursts of white light originating from first and second positions, respectively, are directed towards the spore during a second portion of the time period. After the second portion of the time period has elapsed, the camera shutter is closed to generate an image. The image is analyzed to obtain a shape of the spore.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: October 29, 2019
    Assignee: Scanit Technologies, Inc.
    Inventors: Pedro Manautou, Joel Kent
  • Patent number: 10449537
    Abstract: A system for collection and delivery of sub-milliliter liquid samples is described that provides a dramatic simplification for the measurement of clinical values. The system produces high dilutions of capillary size samples, such as bodily fluids or blood, by a factor of 1000 or more. Multiple assays can be conducted on a sample of 0.1 cc or less. A small lancet can create a drop of blood on a fingertip or other location, which can be collected into a self-priming capillary blood collector. The sample is then loaded into a consumable loader. Then a fluid, such as saline, is flowed into the consumable loader, and passes through the capillary collector, washing out the fluid. Downstream, the blood in the capillary is reliably diluted by mixing with appropriate reagents to allow analysis of selected blood properties. One or more individual bubbles can be inserted into the stream during loading. The capillary loading system can be used for one or several assays on a single blood sample.
    Type: Grant
    Filed: December 13, 2012
    Date of Patent: October 22, 2019
    Assignee: DNA Medicine Institute, Inc.
    Inventors: Julia Z. Sharpe, Eugene Y. Chan
  • Patent number: 10434463
    Abstract: A separation membrane for olefin separation and olefin separation method using the same are provided.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: October 8, 2019
    Assignee: Korea Research Institute of Chemical Technology
    Inventors: Jeong Hoon Kim, Su Young Moon, Bong Jun Chang
  • Patent number: 10436698
    Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: October 8, 2019
    Assignee: FLUIDIGM CORPORATION
    Inventors: Dmitry R. Bandura, Vladimir I. Baranov, Scott D. Tanner
  • Patent number: 10427124
    Abstract: The invention relates to a method of controlling adsorption of lipid molecules onto a solid support by tuning the steric-hydration force of the lipid vesicles and the surface of the solid support, such that the solid support either has a stabilized lipid bilayer adsorbed thereon or is resistant to adsorption of lipid molecules.
    Type: Grant
    Filed: September 19, 2014
    Date of Patent: October 1, 2019
    Assignee: Nanyang Technological University
    Inventors: Nam-Joon Cho, Joshua Alexander Jackman
  • Patent number: 10422797
    Abstract: An electrochemiluminescence method of detecting an analyte in a liquid sample and a corresponding analysis system. An analyte in a liquid sample is detected by first providing a receptacle containing a fluid comprising protein coated magnetic microparticles to a stirring unit. Stirring of the fluid is necessary since the density of the microparticles is usually higher than the density of the buffer fluid. Thus the microparticles tend to deposit on the bottom of the receptacle leading to an aggregation of the microparticles because of weak interactions. To obtain representative measurements a homogeneous distribution of the microparticles in the buffer fluid is necessary to ensure a constant concentration of microparticles for each analysis cycle. It is further necessary to provide disaggregation of the microparticles, which is also realized by stirring the fluid. Stirring is conducted with a rotational frequency that is adapted to the amount of fluid to be stirred.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: September 24, 2019
    Assignees: ROCHE DIAGNOSTICS OPERATIONS, INC., HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Ralf Kraus, Oliver Larbolette, Friedrich Staebler, Yoshihiro Yamashita, Yukinori Sakashita, Shinya Matsuoka, Michihiro Saito, Taku Sakazume, Katsuaki Takahashi
  • Patent number: 10386209
    Abstract: Release liners and methods protect one or more adhesives, wherein the release liners and/or methods comprise at least one abhesive release layer and at least one layer of a getter material capable of sorbing at least one permeable substance, wherein the getter material is at least one substance selected from the group consisting of lithium, beryllium, boron, sodium, magnesium, potassium, calcium, manganese, iron, nickel, zinc, gallium, germanium, cadmium, indium, caesium, barium, boron oxide, calcium oxide, chromium oxide, manganese oxide, iron oxide, copper oxide, silver oxide, indium oxide, barium oxide, lead oxide and mixtures of two or more of the above substances.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: August 20, 2019
    Assignee: TESA SE
    Inventors: Bernd Lühmann, Klaus Keite-Telgenbüscher, Minyoung Bai, Thilo Dollase
  • Patent number: 10379110
    Abstract: The present invention relates to a method for calibrating a multiplex assay, comprising: adding a calibration reagent to a solid phase on which a plurality of capturing agents are immobilized, adding a detection molecule which has a capacity to bind to the calibration reagent, detecting bound detection molecule, thereby creating a calibration curve, wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to a capturing agent immobilized on the solid phase and a capacity to bind to a detection molecule. Further provided is a multiplex assay system comprising such a calibration reagent.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: August 13, 2019
    Assignee: PHADIA AB
    Inventors: Göran Elfverson, Per Matsson, Mats Nystrand
  • Patent number: 10381277
    Abstract: A chip and a method for producing the chip with a plurality of measurement regions which are provided with electrodes for electrically detecting reactions in which, in order to reliably separate the individual measurement regions from one another, a monolayer of a fluorosilane is formed on the chip surface which has strongly hydrophobic properties. Therefore, during spotting with a liquid, the drops of liquid applied by spotting can be reliably prevented from coalescing, and thus, causing mixing of the substances in the drops of liquid which are supposed to be immobilized in the measurement regions.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: August 13, 2019
    Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBH
    Inventors: Markus Schieber, Heinz Schoeder
  • Patent number: 10344320
    Abstract: A capacitive liquid crystal (LC) biosensor for sensing a target biological agent includes a support board; a flow channel on the support board, the flow channel having an inlet port at a first end and an exit port at a second end; at least two electrodes, the at least two electrodes including a first electrode on a flow channel first surface and a second electrode on a flow channel second surface opposite the flow channel first surface; an electricity source connected to the first electrode and the second electrode; and an LC sensor array positioned within the flow channel. An LC sensor array includes a sensor support surface; wells positioned on the sensor support surface; an organic LC phase within each of the plurality of wells; and an aqueous phase having an analyte positioned above the organic LC phase within the wells.
    Type: Grant
    Filed: May 5, 2016
    Date of Patent: July 9, 2019
    Assignee: The Johns Hopkins University
    Inventors: Emily P. English, David B. Taubenheim, Christopher E. Bradburne, Matthew P. Yeager, Jason E. Tiffany, Leslie H. Hamilton
  • Patent number: 10335783
    Abstract: Disclosed is a lateral flow capillary device and uses thereof comprising a unipath bibulous capillary flow matrix and at least two reservoirs each in fluid communication with the capillary flow matrix wherein a reservoir contacts the capillary flow matrix through a passage having a rim pressing the matrix. The pressure that the rim applies on the matrix prevents leakage of liquids out of the capillary flow matrix at the reservoir/matrix interface, allowing accurate sequential draining of liquid from the reservoirs.
    Type: Grant
    Filed: July 13, 2015
    Date of Patent: July 2, 2019
    Assignee: REALBIO TECHNOLOGIES, LTD.
    Inventors: Sara Alajem, Avraham Reinhartz
  • Patent number: 10337982
    Abstract: Methods for determining a sample concentration of target entities in a sample, for example, determining a concentration of target antigens or antibodies in a blood sample or other biological sample.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: July 2, 2019
    Assignee: Biosurfit, S.A.
    Inventor: João Manuel de Oliveira Garcia Da Fonseca
  • Patent number: 10329611
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: June 25, 2019
    Assignee: Intelligent Bio-Systems, Inc.
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 10330667
    Abstract: A method is disclosed involving monitoring the concentration of at least one target analyte in a sample of body fluid using a meter, the meter including a user interface, the method including: obtaining a sample of body fluid; testing the sample to determine the concentration of the at least one target analyte contained therein; and presenting the user with a reminder to associate the test with an appropriate time corresponding to before or after a particular meal using the user interface. Associated devices, systems and arrangements are also disclosed.
    Type: Grant
    Filed: June 24, 2011
    Date of Patent: June 25, 2019
    Assignee: Intuity Medical, Inc.
    Inventors: Kelley J. Lipman, Robin S. Gaffney, Kimberly J. Tansey
  • Patent number: 10330589
    Abstract: Methods for determining a sample concentration of target entities in a sample, for example, determining a concentration of target antigens or antibodies in a blood sample or other biological sample.
    Type: Grant
    Filed: March 22, 2018
    Date of Patent: June 25, 2019
    Assignee: Biosurfit, S.A.
    Inventor: João Manuel de Oliveira Garcia Da Fonseca
  • Patent number: 10325682
    Abstract: The technology described in this document can be embodied in a test strip for use in measuring a level of an ST2 cardiac biomarker in a blood plasma sample. The test strip includes a base, and a plurality of conjugates, wherein each conjugate includes a reporter group bound to a first antibody that binds to ST2. A conjugate pad disposed along a length of the base and is configured to hold the plurality of conjugates that bind with ST2 to produce conjugate-ST2 complexes. The conjugate pad is further configured to receive the blood plasma sample. The test strip also includes a plurality of second and third antibodies that bind to ST2, and the conjugate-ST2 complexes, respectively. The plurality of second antibodies are bound to a membrane in a test location and the plurality of third antibodies are bound to the membrane in a control location.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: June 18, 2019
    Assignee: Critical Care Diagnostics, Inc.
    Inventors: James V. Snider, Jillian Elizabeth Bender, Shrin P. Kuo, Roy A. Chung
  • Patent number: 10302640
    Abstract: Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: May 28, 2019
    Assignee: PLEXBIO CO., LTD.
    Inventors: Dean Tsao, Chin-Shiou Huang, Yao-Kuang Chung
  • Patent number: 10295466
    Abstract: A fluorescence intensity calculating apparatus, includes a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors, and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.
    Type: Grant
    Filed: August 5, 2014
    Date of Patent: May 21, 2019
    Assignee: Sony Corporation
    Inventors: Yasunobu Kato, Yoshitsugu Sakai
  • Patent number: 10281461
    Abstract: The present invention relates to microparticles for analyzing biomolecules, a biomolecule analysis kit comprising the microparticles, and a method for analyzing biomolecules using the analysis kit, the microparticles for analyzing biomolecules comprising: a core including at least one selected from among an optical expression substance, a metallic material, and a magnetic material; a silica coating layer formed on the core; and at least one binding means, linked to the silica coating layer, for binding to an analysis subject biomolecule, wherein the optical expression substance is a fluorescent or a luminescent.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: May 7, 2019
    Assignees: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, DANKOOK UNIVERSITY, Specialty Lab Solution Bio Co.
    Inventor: Heung Bin Lim
  • Patent number: 10281466
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target analyte-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target analyte-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target analyte-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target analyte are also provided.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: May 7, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Neil Percy, Gregory W. Sitton
  • Patent number: 10274415
    Abstract: The present invention regards nano surfaces and particularly a gradient based nano surface. According to embodiments of the invention a surface bound gradient is created by distributed nanoparticles along a plane surface. This procedure greatly reduces the number of prepared surfaces needed, as well as the methodological error of analysis of adsorption and adhesion phenomena.
    Type: Grant
    Filed: December 29, 2016
    Date of Patent: April 30, 2019
    Assignee: CLINE SCIENTIFIC AB
    Inventors: Anders Lundgren, Mattias Berglin, Hans Elwing, Mats Hulander
  • Patent number: 10222349
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: March 5, 2019
    Assignee: QIAGEN SCIENCES, LLC
    Inventors: Steven Gordon, Thomas Hagerott, Edmund Golaski, Jerzy Olejnik
  • Patent number: 10203333
    Abstract: The invention relates to electronically neutral metal complexes as luminescent labels. The positive charge of the metal ion in the complex is neutralized by the negatively charged groups that are covalently linked to the nitrogen-containing diimine ligands, such as 2,2-bipyridine, 1,10-phenanthroline and their derivatives. The electronic neutrality reduces the impact of the metal complexes on the biological and/or biochemical activities of the labeled biomolecules, while the intensity of luminescent emission under electrochemical excitation is enhanced. These luminescent metal complex labels are useful in bioanalytic methodology development, with luminescence as the signal modality, such as electrochemiluminescence.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: February 12, 2019
    Assignee: Rubipy Scientific, Inc.
    Inventors: Ming Zhou, Linpo Yu
  • Patent number: 10190973
    Abstract: An integrated ultraviolet analyzer is described. The integrated ultraviolet analyzer can include one or more ultraviolet analyzer cells, each of which includes one or more ultraviolet photodetectors and one or more solid state light sources, which are monolithically integrated. The solid state light source can be operated to emit ultraviolet light, at least some of which passes through an analyzer active gap and irradiates a light sensing surface of the ultraviolet photodetector. A medium to be evaluated can be present in the analyzer active gap and affect the ultraviolet light as it passes there through, thereby altering an effect of the ultraviolet light on a ultraviolet photodetector.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: January 29, 2019
    Assignee: Sensor Electronic Technology, Inc.
    Inventors: Grigory Simin, Michael Shur, Alexander Dobrinsky
  • Patent number: 10180386
    Abstract: A method for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. Particles or element tags associated with particles can be vaporized, atomized, and ionized, such as with an inductively coupled plasma device or a glow discharge device. The vaporized, atomized, and ionized particles or element tags can be analyzed using mass spectrometry, such as using a time of flight mass spectrometer or a magnetic sector mass spectrometer. The amount of at least one element in individual particles can be measured through mass analysis. The amount of many different tags, for example at least five different tags, can be measured at the same time to facilitate multi-parametric analysis of cells and other particles. The vaporized, atomized, and ionized particles or element tags can be pretreated in an ion pretreatment system to filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: January 15, 2019
    Assignee: Fluidigm Corporation
    Inventors: Dmitry R. Bandura, Vladimir I. Baranov, Scott D. Tanner
  • Patent number: 10180429
    Abstract: Provided are molecule immobilization patterns and a method for forming the same, thereby reducing noise that may occur while analyzing a signal, being stable even at room temperature, and improving orientation of immobilized materials.
    Type: Grant
    Filed: November 25, 2015
    Date of Patent: January 15, 2019
    Assignee: SMALL MACHINES CO., LTD.
    Inventors: Jun Kyu Choi, Jung Hoon Lee, Seung Soo Han, Choo Yeon Kim, Na Hyun Song
  • Patent number: 10174066
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: April 5, 2017
    Date of Patent: January 8, 2019
    Assignee: QIAGEN WALTHAM, Inc.
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 10161000
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: December 25, 2018
    Assignee: QIAGEN WALTHAM, INC.
    Inventor: Jerzy Olejnik
  • Patent number: 10143990
    Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: December 4, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Alexander Mastroianni, Scott C. Benson, Steven M. Menchen, John Scott
  • Patent number: 10137208
    Abstract: Nanostructures, methods of preparing nanostructures, methods of detecting targets in subjects, and methods of treating diseases in subjects, are disclosed. An embodiment, among others, of the nanostructure includes a metallic gold surface-enhanced Raman scattering nanoparticle, a Raman reporter and a protection structure. The protection structure may include a thiol-polyethylene glycol to which may be attached a target-specific probe.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: November 27, 2018
    Assignee: Emory University
    Inventors: Ximei Qian, Dominic Ansari, Shuming Nie
  • Patent number: 10133048
    Abstract: This patent application pertains to the optical laser microscopy field and proposes a simplified yet specific optical laser coupling method. The proposed method allows for a sensible reduction in size and complexity of laser based microscopes and related applications, especially in the area of nano particles detection and optical biosensing. Particularly the optical laser coupling proposed method can detect optical signals generated from sub-diffractive nanoparticles located in liquid solution on a standard glass coverslip. Thanks to the small size of the required components and to the usage of standard air-lens objective, without the presence of oil or special prism, this method can be easily embedded into a small, lightweight and portable device, which can properly operate even in absence of gravity.
    Type: Grant
    Filed: July 9, 2014
    Date of Patent: November 20, 2018
    Assignee: NTP NANO TECH PROJECTS S.R.L.
    Inventor: Adolfo Carloni
  • Patent number: 10101265
    Abstract: Ellipsometers and polarimeters or the like to investigate analyte containing fluids applied to a substrate-stage having a multiplicity of nano-structures that project non-normal to a surface thereof, including dynamics of interaction therewith, to the end of evaluating and presenting at least partial Jones or Mueller Matricies corresponding to a multiplicity of locations over an imaged area.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: October 16, 2018
    Inventors: Mathias M. Schubert, Tino Hofmann, David S. Hage, Erika Pfaunmiller, Craig M. Herzinger, John A. Woollam, Stefan Schoeche
  • Patent number: 10092275
    Abstract: A process for realising a device (1) for collecting and transferring samples for molecular biology, the device including at least: a support body (2) having at least a first portion (2a), and a plurality of fibers (6) attached and arranged on the first portion (2a) of the support body (2) by flocking, such as to define a flocked collecting portion (3) destined to collect, on the said collecting portion, (3) a quantity of the sample for molecular biology, the process including at least a step of pre-treating the plurality of fibers (6) with a surfactant, the step of pre-treating the plurality of fibers (6) with a surfactant being performed after application of the fibers on the support body (2) by flocking and/or before application of the fibers (6) on the body (2) by flocking and/or wherein the surfactant is added to the fiber (6) during production of the fiber (6).
    Type: Grant
    Filed: October 17, 2016
    Date of Patent: October 9, 2018
    Assignee: Copan Italia S.p.A.
    Inventor: Daniele Triva
  • Patent number: 10082512
    Abstract: Methods for diagnosing pathology of the liver in a subject suspected of having such pathology are disclosed. The methods comprise quantifiably detecting glycosylation, and more specifically fucosylation, on proteins in biological fluids, and comparing the detected glycosylation with reference values for the glycosylation of such proteins in healthy or disease states.
    Type: Grant
    Filed: May 5, 2006
    Date of Patent: September 25, 2018
    Assignee: Drexel University
    Inventors: Timothy M. Block, Anand Mehta, Mary Ann Comunale
  • Patent number: 10031106
    Abstract: Method and apparatus for measuring an amount of superparamagnetic material in an object, the method including a) applying a magnetic field having a first component alternating with a first period to the object and a magnetic field strength lower than a magnetic field strength at which the superparamagnetic material is driven in saturation; b) measuring a first magnetic susceptibility of the object with a detection coil; c) applying a static second component to the magnetic field for a second period being equal or larger than the first period, the strength of the magnetic field during the second period is such that the superparamagnetic material is driven towards saturation; d) measuring a second magnetic susceptibility of the object with the detection coil during the application of the static second component; and e) determining the amount of superparamagnetic material from a difference between the measured first and second susceptibility of the object.
    Type: Grant
    Filed: November 19, 2013
    Date of Patent: July 24, 2018
    Assignee: UNIVERSITEIT TWENTE
    Inventors: Sebastiaan Waanders, Martijn Visscher, Tasio Olmo Borendissi Oderkerk, Hendrikus Johannes Gradus Krooshoop, Bernard Haken
  • Patent number: 10024800
    Abstract: The invention relates to Raman spectroscopy-based sensing technique. More particularly, the invention relates to a surface enhanced Raman spectroscopy (SERS) composite and methods of its use and fabrication.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: July 17, 2018
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: Laura Fabris, A. Swarnapali D. S. Indrasekara
  • Patent number: 10009667
    Abstract: A system for automatically monitoring merchandise in a retail sales environment is provided. The system includes a display fixture configured to store and display for sale a group of perishable items and one or more gas emission sensors associated with the display fixture and configured to measure gas emissions from the group of perishable items. The system further includes a control circuit coupled to the one or more gas emission sensors and configured to receive a gas emission measurement taken at the display fixture, compare the gas emission measurement with stored gas emission data associated with a category of the group of perishable items; and make a determination corresponding to the group of perishable items based on the comparison.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: June 26, 2018
    Assignee: Walmart Apollo, LLC
    Inventor: Joseph D. Taylor
  • Patent number: 9999670
    Abstract: The present invention relates to a pharmaceutical composition comprising benzyl alcohol and polyoxyethylene derivatives of sorbitan esters of carboxylic acids that are useful for the delivery of anti-psychotic drugs.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: June 19, 2018
    Assignee: ALKERMES PHARMA IRELAND LIMITED
    Inventors: Jason M. Perry, Magali B. Hickey, Julius F. Remenar, Jennifer Vandiver
  • Patent number: 9994610
    Abstract: The present invention relates to a method for the separation of antibodies, specifically antibodies having different degrees of fucosylation. The method is based on binding affinity of antibodies to Fc receptors. The invention further relates to the use of Fc receptors for the separation of antibodies having different degrees of fucosylation.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: June 12, 2018
    Assignee: ROCHE GLYCART AG
    Inventors: Anne Freimoser-Grundschober, Christiane Jaeger, Peter Sondermann, Pablo Umana
  • Patent number: 9995688
    Abstract: This invention relates to the use of thermodynamically incompatible surfaces in agglutination assays for the express purpose of using the sample as a key component of the detection instrument. Specifically, the invention relates to formation of a lense and a virtual container for rapid mixing via thermal energy by a sample liquid disposed on a superhydrophobic surfaces, and a subsequent specific analyte or overall protein concentration assay using particles agglutination for use in the industrial, environmental, and clinical laboratory test fields.
    Type: Grant
    Filed: August 17, 2010
    Date of Patent: June 12, 2018
    Assignee: Arizona Board of Regents
    Inventors: Antonio Garcia, John Schneider
  • Patent number: 9986733
    Abstract: The present invention provides peptoid polymers capable of reducing or inhibiting the formation of ice crystals at sub 0° C. temperatures. Also provided are peptoid-peptide hybrids comprising the peptoid polymers provided herein. The peptoid polymers and peptoid-peptide hybrids provided herein are useful for making cryoprotectant solutions. The peptoid polymers, peptoid-peptide hybrids, and cryoprotectant solutions provided herein are useful for making antifreeze solutions, frozen food products, and cosmetic care products. Also provided herein are methods for preserving a tissue, an organ, a cell, or a biological macromolecule using the compositions described herein.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: June 5, 2018
    Assignee: X-Therma, Inc.
    Inventor: Xiaoxi Wei
  • Patent number: 9983204
    Abstract: There is disclosed a microarray having base cleavable linkers and a process of making the microarray. The microarray has a solid surface with known locations, each having reactive hydroxyl groups. The density of the known locations is greater than approximately 100 locations per square centimeter. Optionally, oligomers are synthesized in situ onto the cleavable linkers and subsequently cleaved using a cleaving base. Optionally, the oligomers are cleaved and recovered as a pool of oligomers.
    Type: Grant
    Filed: April 27, 2009
    Date of Patent: May 29, 2018
    Assignee: CustomArray, Inc.
    Inventors: Karl Maurer, Dominic Suciu, Hetian Gao
  • Patent number: 9945804
    Abstract: In one illustrative embodiment, a test strip with a first planar substrate has coplanar electrodes on a first planar surface and a second planar substrate (which opposes the first surface of the first planar substrate) has coplanar electrodes on a second planar surface. The first planar surface of the first planar substrate having a first sensing area electrically connected to a first electrical contact. The second planar surface of the second planar substrate having a second electrical contact electrically connected to the first electrical contact via a conductive element, the conductive element extending between the first surface of the first planar substrate and the second surface of the second planar substrate without passing through the first planar substrate, the second planar substrate, or any intermediate layers.
    Type: Grant
    Filed: July 17, 2015
    Date of Patent: April 17, 2018
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Jennifer A. Samproni