Involving An Insoluble Carrier For Immobilizing Immunochemicals Patents (Class 436/518)
  • Patent number: 10993705
    Abstract: The invention relates to a combined two-compartment device for diagnosing body liquids, which comprises: (a) an upper compartment for collecting and storing the body liquid; (b) a lower diagnostic compartment for diagnosing the body liquid at a diagnostic stage, said diagnostic compartment comprises a diagnostic strip; (c) an opening at the bottom wall between said upper compartment and said lower compartment, wherein said opening is kept blocked by a suitable valve until just before said diagnostic stage; and (d) a mechanism for releasing said blockage of said opening, thereby to allow flow of the body liquid to said lower compartment, and thereby to come into contact with said diagnostic strip and to affect the diagnostic strip accordingly.
    Type: Grant
    Filed: November 6, 2016
    Date of Patent: May 4, 2021
    Assignee: NOVAMED LTD.
    Inventors: Emil Katz, Gadi Porath
  • Patent number: 10969324
    Abstract: An acoustic separation device for reagent manipulation a standing acoustic including a wave generating device and pairs of perforated pseudo walls comprising a longitudinal standing bulk acoustic wave (LSBAW) channel. The LSBAW channel provides local pressure field amplification to overcome drag forces arising from reagent flow. Further, method for manipulating a reagent under continuous flow conditions microcarrier particles locally confined in an acoustic separation device comprising an inlet and an outlet, a fluid routing layer coupled to a channel, a plurality of loading wells configured to hold a plurality of substances required for a desired synthesis, and a controller configured to distribute the plurality of substances held by the loading wells in a desired sequence. The device for antibody manipulation further comprises an ultrasound actuator, and a channel wherein the channel is separated from the ultrasound actuator.
    Type: Grant
    Filed: August 16, 2018
    Date of Patent: April 6, 2021
    Assignee: Washington University
    Inventors: Mikhail Berezin, John Mark Meacham, Michael Binkley
  • Patent number: 10969385
    Abstract: The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.
    Type: Grant
    Filed: June 24, 2019
    Date of Patent: April 6, 2021
    Assignee: ZOETIS SERVICES LLC
    Inventors: Rajesh K. Mehra, Kenneth P. Aron
  • Patent number: 10962546
    Abstract: Described herein are methods, compositions and articles of manufacture involving neutral conjugated polymers including methods for synthesis of neutral conjugated water-soluble polymers with linkers along the polymer main chain structure and terminal end capping units. Such polymers may serve in the fabrication of novel optoelectronic devices and in the development of highly efficient biosensors. The invention further relates to the application of these polymers in assay methods.
    Type: Grant
    Filed: May 28, 2020
    Date of Patent: March 30, 2021
    Assignee: SIRIGEN II LIMITED
    Inventors: Brent S. Gaylord, Glenn P. Bartholomew, Russell A. Baldocchi, Janice W. Hong, William H. Huisman, Yongchao Liang, Trung Nguyen, Lan T. Tran, Jean M. Wheeler, Adrian Charles Vernon Palmer, Frank Peter Uckert
  • Patent number: 10961574
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: November 5, 2019
    Date of Patent: March 30, 2021
    Assignee: QIAGEN SCIENCES, LLC
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 10956930
    Abstract: Dynamic Hierarchical Empirical Bayes techniques and systems are described that are implemented to control output of digital content. In one example, a system identifies splitting variables included in data. An amount of loss is then determined for each of the identified splitting variables by the system using a loss function. Based on the determined amounts of loss, the system selects at least one splitting variable from the plurality of splitting variables that are to be used to partition data in a respective node, e.g., a parent node to form a plurality of child nodes. The system, for instance, may select the splitting variable that minimizes the cost, i.e., has the lowest amount of cost. The selected splitting variable is then employed by the system to generate at least one hierarchical level of the hierarchical structure of the statistical model by partitioning data from the parent node into respective child nodes.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: March 23, 2021
    Assignee: Adobe Inc.
    Inventors: Yuan Yuan, Zhenyu Yan, Yiwen Sun, Xiaojing Dong, Chen Dong, Abhishek Pani
  • Patent number: 10948500
    Abstract: Methods and kits-of-parts for determining the quantity of HbA1c relative to the concentration of haemoglobin in a blood sample which includes less than 200 ?l of blood, the method including the steps of adding the fluorophore to the sample and measuring the fluorescence at one or more time points within the time interval, at which the change in fluorescence over time is >0, followed by measurements of haemoglobin by adding a haemoglobin-binding agent and measuring the change in transmission at approximately 570 nm and comparing the obtained results with an internal standard.
    Type: Grant
    Filed: December 29, 2016
    Date of Patent: March 16, 2021
    Assignee: W. Health L.P.
    Inventor: Peter Warthoe
  • Patent number: 10941319
    Abstract: Process for printing an adhesive pattern on a polymer brush extending at the surface of a support (1), forming a nanometric anti-fouling layer (2), the process comprising the following steps: —placing the layer (2) in contact with a first aqueous solution (4) containing a benzophenone, —then illuminating the layer with radiation (3) at a wavelength within the absorption spectrum of benzophenone, according to the pattern and according to a surface energy.
    Type: Grant
    Filed: October 2, 2015
    Date of Patent: March 9, 2021
    Assignees: ALVEOLE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE—CNRS, UNIVERSITÉ DE BORDEAUX
    Inventor: Vincent Studer
  • Patent number: 10914738
    Abstract: A subtractive immunoassay for detecting the presence or absence of first and second analytes in a sample includes in the following order the steps of: a) conjugating a first detector antibody to the first analyte to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte to capture the first analyte to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte to capture the second analyte from the sample; e) detecting presence or absence of the first and second detector tags at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first and second analytes in the sample.
    Type: Grant
    Filed: October 24, 2016
    Date of Patent: February 9, 2021
    Inventors: Thomas Bruderer, Madhavi Muranjan
  • Patent number: 10864359
    Abstract: A medical delivery device includes a first compartment configured to hold at least a portion of an activator that activates one or more molecular nanomachines. The first compartment includes a first wall that includes a first ferrous material, where the first wall is configured to disintegrate in response to first electromagnetic radiation received by the first ferrous material such that the activator activates the one or more molecular nanomachines. The device includes a second compartment configured to hold the one or more molecular nanomachines, wherein the second compartment includes a second wall that includes a second ferrous material, and wherein the second wall is configured to disintegrate and release the one or more molecular nanomachines into a patient in response to second electromagnetic radiation received by the second ferrous material.
    Type: Grant
    Filed: January 22, 2020
    Date of Patent: December 15, 2020
    Inventor: Bernard Fryshman
  • Patent number: 10858429
    Abstract: The present invention relates to methods for preventing or treating head and neck spuamous cell cancer and bladder cancer using an immunotoxin comprising (a) a ligand that binds to a protein on the cancer cell attached to; (b) a toxin that is cytotoxic to the cancer cell. In a specific embodiment, the invention is directed to the prevention or treatment of head and neck squamous cell cancer or bladder cancer using Vb4-845, which is a recombinant immunotixin comprising a humanized, MOC31-derived, single-chain antibody fragment that is fused to a truncated form of Pseudomonas exotoxin A. Also encompassed by the invention are combination therapy methods, including the use of reduced dosages of chemotherapeutic agents, for the prevention or treatment of cancer. Also encompassed by the invention are formulations and methods for direct administration of the recombinant immunotoxin to the carcinoma, for the prevention or treatment of cancer.
    Type: Grant
    Filed: January 7, 2016
    Date of Patent: December 8, 2020
    Assignee: UNIVERSITY OF ZURICH
    Inventors: Uwe Zangemeister-Wittke, Claudio Di Paolo, Dominique Christine Tschudi, Nicholas Ronald Glover, Dimitri Peter Fitsialos
  • Patent number: 10830842
    Abstract: It is an object of the invention to provide for an improved method for device localization using magnetic resonance imaging (MRI) during MRI guided interventions.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: November 10, 2020
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventor: Sascha Krueger
  • Patent number: 10828381
    Abstract: The presently-disclosed subject matter relates to an artificial RNA nanostructure and method of use thereof. In particular, the presently-disclosed subject matter relates to RNA nanoparticles and RNA dendrimers, and methods of disease diagnosis and treatments using the RNA nanostructure and RNA dendrimers.
    Type: Grant
    Filed: April 17, 2016
    Date of Patent: November 10, 2020
    Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION
    Inventors: Peixuan Guo, Hui Li, Wei Luo
  • Patent number: 10807087
    Abstract: The invention concerns a lateral flow device, system, kit and method for analyzing a fluid sample. The device comprises a support structure, a flow channel formed in the support structure, and an injection zone in fluidic connection with the flow channel for introducing the fluid sample into the flow channel. According to the invention the flow channel comprises at least two indicator zones at least one of which is capable of producing an optically detectable response signal when interacting with the fluid sample, and the indicator zones are arranged at least partly adjacent to each other on different sections of the flow channel. The invention allows for low-cost imaging devices, such as cameras of mobile phones to be used for making challenging lateral flow diagnostics on a variety of fields of technology.
    Type: Grant
    Filed: April 13, 2016
    Date of Patent: October 20, 2020
    Assignee: Teknologian tutkimuskeskus VTT Oy
    Inventors: Jukka-Tapani Mäkinen, Sanna Uusitalo, Marika Kurkinen
  • Patent number: 10745740
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: May 20, 2013
    Date of Patent: August 18, 2020
    Assignee: Qiagen Sciences, LLC
    Inventors: Steven Gordon, Phillip A Veatch
  • Patent number: 10739297
    Abstract: A lateral flow assay device comprising a conjugate pad for receiving a quantity of fluid; and a membrane comprising a test line for determining whether the fluid comprises a target analyte. In a first state of the lateral flow assay device, the lateral flow assay device is configured with a removable gap between the conjugate pad and the membrane which is substantially filled with air and prevents the fluid from flowing from the conjugate pad into the membrane. In a second state of the lateral flow assay device, the removable gap is removed from between the conjugate pad and the membrane causing the conjugate pad to come in contact with the membrane, allowing the fluid to flow from the conjugate pad into the membrane and the test line by capillary action.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: August 11, 2020
    Assignee: 2Pi-Sigma Corp.
    Inventor: Mehdi Hatamian
  • Patent number: 10739366
    Abstract: A continuous throughput microfluidic system includes an input system configured to provide a sequential stream of sample plugs; a droplet generator arranged in fluid connection with the input system to receive the sequential stream of sample plugs and configured to provide an output stream of droplets; a droplet treatment system arranged in fluid connection with the droplet generator to receive the output stream of droplets in a sequential order and configured to provide a stream of treated droplets in the sequential order; a detection system arranged to obtain detection signals from the treated droplets in the sequential order; a control system configured to communicate with the input system, the droplet generator, and the droplet treatment system; and a data processing and storage system configured to communicate with the control system and the detection system.
    Type: Grant
    Filed: January 18, 2019
    Date of Patent: August 11, 2020
    Assignee: The Johns Hopkins University
    Inventors: Tza-huei J. Wang, Tushar Dnyandeo Rane, Helena Claire Zec, Wen-Chy Chu
  • Patent number: 10725048
    Abstract: Disclosed is a method for detecting a test substance, including a first contact step of bringing the test substance, a capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and a capturing body 2 that has binding properties to the test substance and contains a label into contact with each other in a liquid to arrange at least a part of the capturing body 2 on the substrate; after the first contact step, a first dissociation step of dissociating a part of substances containing the capturing body 2 from the substrate and releasing it into the liquid; after the first dissociation step, a second contact step of bringing the test substance, the capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and the capturing body 2 that has binding properties to the test substance and contains a label into contact with each other in the liquid to arrange at least a part of the capturing body 2 on the substrate; after the second contact step,
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: July 28, 2020
    Assignee: SYSMEX CORPORATION
    Inventors: Kenji Akama, Hiroya Kirimura
  • Patent number: 10725031
    Abstract: Devices for detection of analytes in samples of various complexities are provided. The devices allow reflux of liquid into a sample-receiving member, providing for suitable analyte dilution and preventing leakage of cells or particulate material into the signal generation member. In one aspect, the devices are used to test analytes in biological samples such as blood, serum, plasma or other body fluids. In another aspect, the devices are used to test samples from tissues, such as organ tissues, tumor samples, or other body parts. In a further aspect, the devices are used to test microbial samples, food samples, or any other sample from which the analyte of interest can be captured into the reflux device.
    Type: Grant
    Filed: March 22, 2018
    Date of Patent: July 28, 2020
    Assignee: ConquerAb Inc.
    Inventor: Maria D. F. S. Barbosa
  • Patent number: 10722882
    Abstract: The disclosure provides compositions, kits and methods comprising an absorbent pad comprising a fibrous material and a liquid formulation comprising a luminescent substrate for identifying and quantifying biomolecule analytes.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: July 28, 2020
    Assignee: Pierce Biotechnology, Inc.
    Inventors: Surbhi Desai, Kari Severson
  • Patent number: 10716867
    Abstract: A composition includes a plurality of gold nanoparticles each having at least one surface. The gold nanoparticles have an average length of at least about 90 nm and an average width of at least about 25 nm.
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: July 21, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Adam De La Zerda, Orly Liba, Elliott Sorelle, Bryan Knysh
  • Patent number: 10684277
    Abstract: A method for colorimetric detection of cholesterol in a sample is disclosed. The method includes adding beta-cyclodextrin and cholesterol to a phenolphthalein indicator solution in the presence of a phosphate buffer solution to create a solution medium and quantifying the cholesterol as a function of a complexed beta-cyclodextrin in the solution medium.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: June 16, 2020
    Assignee: COMSATS Institute of Information Technology
    Inventors: Akhtar Hayat, Muhammad Azhar Hayat Nawaz
  • Patent number: 10670615
    Abstract: A system for determining a concentration of hemoglobin A1C includes a first lateral flow test strip, the first lateral flow test strip providing for a percent of HbA1C concentration; a second lateral flow test strip, the second lateral flow test strip providing for the total amount of hemoglobin; an antibody-microparticle stripe on each of the first and second lateral flow test strips; a conjugate stripe on each of the first and second lateral flow test strips; and a sample treatment buffer. The sample treatment buffer is strongly denaturing, and antibodies in the antibody-microparticle strip are covalently bound to microparticles.
    Type: Grant
    Filed: December 5, 2016
    Date of Patent: June 2, 2020
    Assignee: Polymer Technology Systems, Inc.
    Inventors: Charles Z. Xie, Jane Kyung, Tu-Anh Nguyen, Annie V. Mendoza, Keith Moskowitz
  • Patent number: 10653017
    Abstract: The present disclosure provides a glass layer for a display screen and a display screen, including: a glass layer body having a transverse direction and a longitudinal direction perpendicular to each other; a plurality of ribs spaced apart from each other and arranged on an upper surface of the glass layer body, a groove being formed between every two adjacent said ribs; a movable brush arranged on an outer surface of each of said ribs; a drive connection mechanism connecting with the movable brush for driving the movable brush to be reciprocated on the outer surface of said ribs.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: May 12, 2020
    Assignee: BOE TECHNOLOGY GROUP CO., LTD.
    Inventor: Xiao Chu
  • Patent number: 10634602
    Abstract: The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: April 28, 2020
    Assignee: CYTOCHIP INC.
    Inventors: Wendian Shi, Yuzhe Ding
  • Patent number: 10555886
    Abstract: A composition of algal extracts containing phycobiliprotein is provided. This composition not only effectively inhibit the growth of oral pathogens including Porphyromonathes gingivalis and Actinobacillus actinomycetemcomitans, but also reduces the inflammation caused by those pathogens. Therefore, the phenomenon of alveolar bone loss in periodontal patients is mitigation, achieving the effect of prevention and treatment of periodontal disease.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: February 11, 2020
    Assignee: FAR EAST BIO-TEC CO., LTD.
    Inventors: Tz-Chong Chou, Chuang-Chun Chiuh
  • Patent number: 10539559
    Abstract: Disclosed are a PIVKA-II measurement method that achieves better serum-plasma correlation than conventional methods, and a reagent and a kit therefor. The PIVKA-II measurement method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using a mixture of an anti-F1 antibody that specifically binds to prothrombin fragment F1 or an antigen-binding fragment thereof and an anti-F2 antibody that specifically binds to prothrombin fragment F2 or an antigen-binding fragment thereof; and an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof.
    Type: Grant
    Filed: August 6, 2013
    Date of Patent: January 21, 2020
    Assignees: FUJIREBIO INC., SEKISUI MEDICAL CO., LTD.
    Inventors: Kentaro Yamaguchi, Katsumi Aoyagi, Azusa Terao
  • Patent number: 10518267
    Abstract: Embodiments of the invention provide a filter kit including filters for processing a biological sample. Some embodiments include a filter cap in a tube kit with a first tube containing a buffer solution and a second tube containing a lyophilized master mix. Some embodiments include a method of processing a sample using the kit including mixing a biological sample in a first tube with the buffer solution, positioning the filter cap in the first tube, positioning a second tube on the filter cap, flipping the first tube, the filter cap, and the second cap to filter the biological sample and buffer solution mixture with the filter cap as it flows from the first tube to the second tube. Some embodiments include structure enabling transfer of materials through inline flow between the tubes. Some further embodiments include integrated structure for sample pulverization with integrated buffer and lyophilized master mix.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: December 31, 2019
    Assignee: DOUGLAS SCIENTIFIC, LLC
    Inventors: Robert Hjelseth, Cory Hodgson, Andrew Bristow, Mark Baumgartner, David Sellnow, Cassie Keppel, John Steckelberg, Paul Newman
  • Patent number: 10495634
    Abstract: The present invention provides a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a liquid sample by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD4+, CD8+ cells, malaria-infected red blood cells, cancer cells, cancer biomarkers such as prostate specific antigen and other cancer biomarkers, viruses, bacteria, and other pathogenic agents, with the sensitivity, specificity and accuracy of bench-top laboratory-based assays.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: December 3, 2019
    Assignee: Carnegie Mellon University
    Inventors: Alberto Gandini, James F. Antaki, Byron Wang Chuan, Joie N. Marhefka
  • Patent number: 10472382
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable Media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: November 12, 2019
    Assignee: QIAGEN SCIENCES, LLC
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 10458990
    Abstract: A flow of air including a fungal spore is directed to a collection cartridge. The spore is trapped within the cartridge and positioned within a field of view of a camera sensor. A UV light is activated to illuminate the spore and a camera shutter is opened for a time period. The camera sensor collects light emitted from the spore during a first portion of the time period. After the first portion has elapsed, first and second bursts of white light originating from first and second positions, respectively, are directed towards the spore during a second portion of the time period. After the second portion of the time period has elapsed, the camera shutter is closed to generate an image. The image is analyzed to obtain a shape of the spore.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: October 29, 2019
    Assignee: Scanit Technologies, Inc.
    Inventors: Pedro Manautou, Joel Kent
  • Patent number: 10449537
    Abstract: A system for collection and delivery of sub-milliliter liquid samples is described that provides a dramatic simplification for the measurement of clinical values. The system produces high dilutions of capillary size samples, such as bodily fluids or blood, by a factor of 1000 or more. Multiple assays can be conducted on a sample of 0.1 cc or less. A small lancet can create a drop of blood on a fingertip or other location, which can be collected into a self-priming capillary blood collector. The sample is then loaded into a consumable loader. Then a fluid, such as saline, is flowed into the consumable loader, and passes through the capillary collector, washing out the fluid. Downstream, the blood in the capillary is reliably diluted by mixing with appropriate reagents to allow analysis of selected blood properties. One or more individual bubbles can be inserted into the stream during loading. The capillary loading system can be used for one or several assays on a single blood sample.
    Type: Grant
    Filed: December 13, 2012
    Date of Patent: October 22, 2019
    Assignee: DNA Medicine Institute, Inc.
    Inventors: Julia Z. Sharpe, Eugene Y. Chan
  • Patent number: 10434463
    Abstract: A separation membrane for olefin separation and olefin separation method using the same are provided.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: October 8, 2019
    Assignee: Korea Research Institute of Chemical Technology
    Inventors: Jeong Hoon Kim, Su Young Moon, Bong Jun Chang
  • Patent number: 10436698
    Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: October 8, 2019
    Assignee: FLUIDIGM CORPORATION
    Inventors: Dmitry R. Bandura, Vladimir I. Baranov, Scott D. Tanner
  • Patent number: 10427124
    Abstract: The invention relates to a method of controlling adsorption of lipid molecules onto a solid support by tuning the steric-hydration force of the lipid vesicles and the surface of the solid support, such that the solid support either has a stabilized lipid bilayer adsorbed thereon or is resistant to adsorption of lipid molecules.
    Type: Grant
    Filed: September 19, 2014
    Date of Patent: October 1, 2019
    Assignee: Nanyang Technological University
    Inventors: Nam-Joon Cho, Joshua Alexander Jackman
  • Patent number: 10422797
    Abstract: An electrochemiluminescence method of detecting an analyte in a liquid sample and a corresponding analysis system. An analyte in a liquid sample is detected by first providing a receptacle containing a fluid comprising protein coated magnetic microparticles to a stirring unit. Stirring of the fluid is necessary since the density of the microparticles is usually higher than the density of the buffer fluid. Thus the microparticles tend to deposit on the bottom of the receptacle leading to an aggregation of the microparticles because of weak interactions. To obtain representative measurements a homogeneous distribution of the microparticles in the buffer fluid is necessary to ensure a constant concentration of microparticles for each analysis cycle. It is further necessary to provide disaggregation of the microparticles, which is also realized by stirring the fluid. Stirring is conducted with a rotational frequency that is adapted to the amount of fluid to be stirred.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: September 24, 2019
    Assignees: ROCHE DIAGNOSTICS OPERATIONS, INC., HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Ralf Kraus, Oliver Larbolette, Friedrich Staebler, Yoshihiro Yamashita, Yukinori Sakashita, Shinya Matsuoka, Michihiro Saito, Taku Sakazume, Katsuaki Takahashi
  • Patent number: 10386209
    Abstract: Release liners and methods protect one or more adhesives, wherein the release liners and/or methods comprise at least one abhesive release layer and at least one layer of a getter material capable of sorbing at least one permeable substance, wherein the getter material is at least one substance selected from the group consisting of lithium, beryllium, boron, sodium, magnesium, potassium, calcium, manganese, iron, nickel, zinc, gallium, germanium, cadmium, indium, caesium, barium, boron oxide, calcium oxide, chromium oxide, manganese oxide, iron oxide, copper oxide, silver oxide, indium oxide, barium oxide, lead oxide and mixtures of two or more of the above substances.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: August 20, 2019
    Assignee: TESA SE
    Inventors: Bernd Lühmann, Klaus Keite-Telgenbüscher, Minyoung Bai, Thilo Dollase
  • Patent number: 10381277
    Abstract: A chip and a method for producing the chip with a plurality of measurement regions which are provided with electrodes for electrically detecting reactions in which, in order to reliably separate the individual measurement regions from one another, a monolayer of a fluorosilane is formed on the chip surface which has strongly hydrophobic properties. Therefore, during spotting with a liquid, the drops of liquid applied by spotting can be reliably prevented from coalescing, and thus, causing mixing of the substances in the drops of liquid which are supposed to be immobilized in the measurement regions.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: August 13, 2019
    Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBH
    Inventors: Markus Schieber, Heinz Schoeder
  • Patent number: 10379110
    Abstract: The present invention relates to a method for calibrating a multiplex assay, comprising: adding a calibration reagent to a solid phase on which a plurality of capturing agents are immobilized, adding a detection molecule which has a capacity to bind to the calibration reagent, detecting bound detection molecule, thereby creating a calibration curve, wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to a capturing agent immobilized on the solid phase and a capacity to bind to a detection molecule. Further provided is a multiplex assay system comprising such a calibration reagent.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: August 13, 2019
    Assignee: PHADIA AB
    Inventors: Göran Elfverson, Per Matsson, Mats Nystrand
  • Patent number: 10344320
    Abstract: A capacitive liquid crystal (LC) biosensor for sensing a target biological agent includes a support board; a flow channel on the support board, the flow channel having an inlet port at a first end and an exit port at a second end; at least two electrodes, the at least two electrodes including a first electrode on a flow channel first surface and a second electrode on a flow channel second surface opposite the flow channel first surface; an electricity source connected to the first electrode and the second electrode; and an LC sensor array positioned within the flow channel. An LC sensor array includes a sensor support surface; wells positioned on the sensor support surface; an organic LC phase within each of the plurality of wells; and an aqueous phase having an analyte positioned above the organic LC phase within the wells.
    Type: Grant
    Filed: May 5, 2016
    Date of Patent: July 9, 2019
    Assignee: The Johns Hopkins University
    Inventors: Emily P. English, David B. Taubenheim, Christopher E. Bradburne, Matthew P. Yeager, Jason E. Tiffany, Leslie H. Hamilton
  • Patent number: 10335783
    Abstract: Disclosed is a lateral flow capillary device and uses thereof comprising a unipath bibulous capillary flow matrix and at least two reservoirs each in fluid communication with the capillary flow matrix wherein a reservoir contacts the capillary flow matrix through a passage having a rim pressing the matrix. The pressure that the rim applies on the matrix prevents leakage of liquids out of the capillary flow matrix at the reservoir/matrix interface, allowing accurate sequential draining of liquid from the reservoirs.
    Type: Grant
    Filed: July 13, 2015
    Date of Patent: July 2, 2019
    Assignee: REALBIO TECHNOLOGIES, LTD.
    Inventors: Sara Alajem, Avraham Reinhartz
  • Patent number: 10337982
    Abstract: Methods for determining a sample concentration of target entities in a sample, for example, determining a concentration of target antigens or antibodies in a blood sample or other biological sample.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: July 2, 2019
    Assignee: Biosurfit, S.A.
    Inventor: João Manuel de Oliveira Garcia Da Fonseca
  • Patent number: 10329611
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: June 25, 2019
    Assignee: Intelligent Bio-Systems, Inc.
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 10330667
    Abstract: A method is disclosed involving monitoring the concentration of at least one target analyte in a sample of body fluid using a meter, the meter including a user interface, the method including: obtaining a sample of body fluid; testing the sample to determine the concentration of the at least one target analyte contained therein; and presenting the user with a reminder to associate the test with an appropriate time corresponding to before or after a particular meal using the user interface. Associated devices, systems and arrangements are also disclosed.
    Type: Grant
    Filed: June 24, 2011
    Date of Patent: June 25, 2019
    Assignee: Intuity Medical, Inc.
    Inventors: Kelley J. Lipman, Robin S. Gaffney, Kimberly J. Tansey
  • Patent number: 10330589
    Abstract: Methods for determining a sample concentration of target entities in a sample, for example, determining a concentration of target antigens or antibodies in a blood sample or other biological sample.
    Type: Grant
    Filed: March 22, 2018
    Date of Patent: June 25, 2019
    Assignee: Biosurfit, S.A.
    Inventor: João Manuel de Oliveira Garcia Da Fonseca
  • Patent number: 10325682
    Abstract: The technology described in this document can be embodied in a test strip for use in measuring a level of an ST2 cardiac biomarker in a blood plasma sample. The test strip includes a base, and a plurality of conjugates, wherein each conjugate includes a reporter group bound to a first antibody that binds to ST2. A conjugate pad disposed along a length of the base and is configured to hold the plurality of conjugates that bind with ST2 to produce conjugate-ST2 complexes. The conjugate pad is further configured to receive the blood plasma sample. The test strip also includes a plurality of second and third antibodies that bind to ST2, and the conjugate-ST2 complexes, respectively. The plurality of second antibodies are bound to a membrane in a test location and the plurality of third antibodies are bound to the membrane in a control location.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: June 18, 2019
    Assignee: Critical Care Diagnostics, Inc.
    Inventors: James V. Snider, Jillian Elizabeth Bender, Shrin P. Kuo, Roy A. Chung
  • Patent number: 10302640
    Abstract: Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: May 28, 2019
    Assignee: PLEXBIO CO., LTD.
    Inventors: Dean Tsao, Chin-Shiou Huang, Yao-Kuang Chung
  • Patent number: 10295466
    Abstract: A fluorescence intensity calculating apparatus, includes a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors, and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.
    Type: Grant
    Filed: August 5, 2014
    Date of Patent: May 21, 2019
    Assignee: Sony Corporation
    Inventors: Yasunobu Kato, Yoshitsugu Sakai
  • Patent number: 10281466
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target analyte-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target analyte-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target analyte-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target analyte are also provided.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: May 7, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Neil Percy, Gregory W. Sitton
  • Patent number: 10281461
    Abstract: The present invention relates to microparticles for analyzing biomolecules, a biomolecule analysis kit comprising the microparticles, and a method for analyzing biomolecules using the analysis kit, the microparticles for analyzing biomolecules comprising: a core including at least one selected from among an optical expression substance, a metallic material, and a magnetic material; a silica coating layer formed on the core; and at least one binding means, linked to the silica coating layer, for binding to an analysis subject biomolecule, wherein the optical expression substance is a fluorescent or a luminescent.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: May 7, 2019
    Assignees: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, DANKOOK UNIVERSITY, Specialty Lab Solution Bio Co.
    Inventor: Heung Bin Lim