Antigen Or Antibody Entrapped Within The Carrier (e.g., Gel, Hollow Fiber, Etc.) Patents (Class 436/535)
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Patent number: 7226753Abstract: The present invention relates to displacement assay type bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyvinylchloride or polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.Type: GrantFiled: January 28, 2004Date of Patent: June 5, 2007Assignee: Toxin Alert, Inc.Inventor: William T. Bodenhamer
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Patent number: 7211444Abstract: A fluidic waveguide comprising a container and a fluid that fills said container, wherein said fluid has a refractive index greater than the refractive index of the wall of said container and wherein said fluid can act as a waveguide for electromagnetic radiation when contacted therewith is disclosed. A corresponding fluidic lightguide along with devices that function as composite waveguides and lightguides are described. Assays utilizing this waveguide for biochemical, chemical, and other kinds of analyzes are also disclosed.Type: GrantFiled: April 26, 2002Date of Patent: May 1, 2007Assignee: Genetic ID NA, Inc.Inventor: John Fagan
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Patent number: 7205162Abstract: Artificial antibodies or antibody mimics are described. They consist of polymers that carry specific binding sites mimicking the properties of antibodies. There is also described a method for producing artificial antibodies, in which polymerisable monomers carrying functional groups and crosslinking monomers are polymerised in the presence of a print molecule and subsequently the print molecule is removed leaving specific binding sites complementary to the print molecules. There are also described methods for determination and isolation of organic molecules using the artificial antibodies as well as therapeutic and diagnostic methods using these antibodies.Type: GrantFiled: May 6, 1999Date of Patent: April 17, 2007Inventors: Klaus Mosbach, Georg Vlatakis, Lars I. Andersson, Ralf Müller
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Patent number: 7153682Abstract: Provided are peptidomimetic protein-binding arrays, their manufacture, use, and application. The protein-binding array elements of the invention include a peptidomimetic segment linked to a solid support via a stable anchor. The invention contemplates peptidomimetic array element library synthesis, distribution, and spotting of array elements onto solid planar substrates, labeling of complex protein mixtures, and the analysis of differential protein binding to the array. The invention also enables the enrichment or purification, and subsequent sequencing or structural analysis of proteins that are identified as differential by the array screen. Kits including proteomic microarrays in accordance with the present invention are also provided.Type: GrantFiled: July 3, 2002Date of Patent: December 26, 2006Assignee: Chiron CorporationInventors: Deborah Charych, Eric Beausoleil, Ronald N. Zuckermann
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Patent number: 7148058Abstract: Provided are protein microarrays, their manufacture, use, and application. Protein microarrays in accordance with the present invention are useful in a variety preoteomic analyses. Various protein arrays in accordance with the present invention may immobilize large arrays of proteins that may be useful for studying protein-protein interactions to improve understanding of disease processes, facilitating drug discovery, or for identifying potential antigens for vaccine development. The protein array elements of the invention are native or modified proteins (e.g., antibodies or fusion proteins). The protein array elements may be attached directly to a organic functionalized mirrored substrate by a binding reaction between functional groups on the substrate (e.g., amine) and protein (e.g., activated carboxylic acid). Techniques for chemical blocking of the arrays are also provided.Type: GrantFiled: July 3, 2002Date of Patent: December 12, 2006Assignee: Chiron CorporationInventors: Deborah Charych, Ronald N. Zuckermann
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Patent number: 7141436Abstract: A sandwich immunoassay is disclosed that provides simple to perform yet sensitive identification of analytes in samples. All assay constituents needed (except analyte to be detected) for one assay are dried. Upon reconstitution with sample, a 10 to 15 minute incubation gives a rapid and convenient detection assay capability. The method incorporates the capture of antigen to an immobilized capture antibody. A labeled reporter antibody with the molecule, binds to the antigen to form an immunocomplex capable of generating a detectable signal.Type: GrantFiled: May 20, 2002Date of Patent: November 28, 2006Assignee: Science and Technology Corp.Inventors: Deborah L. Gatto-Menking, Michael T. Goode
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Patent number: 7135300Abstract: Methods are provided for detecting formation of oligomeric complexes of molecules on the surface of cell membranes. These methods employ pairs of tagged probes and cleaving probes, each of which binds specificly to a cell surface molecule. The tagged probe includes a molecular tag that is linked to a first binding compound through a cleavable linkage, and the cleaving probe includes a second binding agent and a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to cell surface molecules that have formed an oligomeric complex results in release of the molecular tag from the binding compound, providing a measure of formation of the complex.Type: GrantFiled: April 22, 2004Date of Patent: November 14, 2006Assignee: Monogram Biosciences, Inc.Inventors: Po-Ying Chan-Hui, Yining Shi, Sailaja Pidaparthi, Rajiv Dua, Sharat Singh
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Patent number: 7118900Abstract: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention further relates to the incorporation of said assemblies into other structures. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films. This invention also provides simple methods and apparatus for synthesizing thin films of tailored composition and morphology.Type: GrantFiled: June 21, 2002Date of Patent: October 10, 2006Assignee: BioArray Solutions Ltd.Inventors: Michael Seul, Sukanta Banerjee, Kairali Podual, Ye Hong
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Patent number: 7112453Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 5, 2002Date of Patent: September 26, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7105308Abstract: Methods are provided for detecting formation of oligomeric complexes of molecules on the surface of cell membranes. These methods employ pairs of tagged probes and cleaving probes, each of which binds specificly to a cell surface molecule. The tagged probe includes a molecular tag that is linked to a first binding compound through a cleavable linkage, and the cleaving probe includes a second binding agent and a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to cell surface molecules that have formed an oligomeric complex results in release of the molecular tag from the binding compound, providing a measure of formation of the complex.Type: GrantFiled: July 17, 2003Date of Patent: September 12, 2006Assignee: Monogram Biosciences, Inc.Inventors: Po-Ying Chan-Hui, Yining Shi, Sailaja Pidaparthi, Rajiv Dua, Sharat Singh
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Patent number: 7105289Abstract: The present invention provides imprint bead compositions useful for capturing, isolating, detecting, analyzing and/or quantifying molecules in a sample. The imprint bead compositions comprise a matrix material having imprint cavities of a template molecule or molecules imprinted thereon wherein a substantial number of the imprint cavities are located at or near the surface of the matrix material.Type: GrantFiled: April 11, 2002Date of Patent: September 12, 2006Assignee: Aspira Biosystems, Inc.Inventors: Chin-Shiou Huang, Casey C. Lynch, Alexander Strikovsky
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Patent number: 7097983Abstract: The invention features a method of determining whether one or more members of an analyte family are present in a sample. The method makes use of a test zone binder, e.g., a receptor that can bind one or a plurality of analytes within an analyte family, the analytes family defined by similar structural binding sites. Members of an analyte family can have different detection level requirements and, therefore, additional analyte binders can be employed to adjust test sensitivity for a subset of the analytes in the analyte family individually, so that each analyte can be detected only if it is present in the sample above a predetermined threshold.Type: GrantFiled: September 24, 2001Date of Patent: August 29, 2006Assignee: Charm Sciences, Inc.Inventors: Robert J. Markovsky, Cheryl A. Boyer, Stanley E. Charm, Paul R. Donahue, Yael Agi Glickman, Steven J. Saul, Joan L. Scheemaker, Richard T. Skiffington, Shefali B. Trivedi, Eliezer Zomer
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Patent number: 7063952Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: GrantFiled: September 28, 2001Date of Patent: June 20, 2006Assignee: Bayer Healthcare AGInventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
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Patent number: 7052854Abstract: Systems and methods for the ex vivo diagnostic analysis of samples of bodily fluids, including exhaled breath and blood. The present invention uses nanostructure-based assemblies in combination with sensor technology to provide an efficient and accurate means for identifying the presence of a target analyte/biomarker in a sample of bodily fluid. In a preferred embodiment, the nanostructure-based assemblies of the present invention include detecting means such as RNA oligonucleotide chains or “apparatus” and releasable surrogate markers such as DMSO.Type: GrantFiled: October 2, 2003Date of Patent: May 30, 2006Assignee: University of Florida Research Foundation, Inc.Inventors: Richard J. Melker, Ronald L. Hayes, Ka-Wang Kevin Wang, Donn Michael Dennis
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Patent number: 7049152Abstract: The present invention herein provides the design, synthesis and characterization of compositions comprising asymmetric bolaamphiphilic lipids that form extended polymeric ribbons and wide sheets. These compositions may be doped, or interspersed, with various compounds to fine-tune the fluidity and rigidity of the bolaamphiphilic lipid composition, and promote other morphologies of the composition, including fluid vesicles and truncated flat sheets. Upon an increase in pH these compositions undergo a calorimetric and morphological transformation.Type: GrantFiled: March 12, 2002Date of Patent: May 23, 2006Assignee: The Regents of the University of CaliforniaInventors: Raymond C. Stevens, Quan Cheng, Jie Song
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Patent number: 7049150Abstract: A binding assay device includes a porous membrane comprising a material enabling capillary movement of a liquid sample from a first area on the membrane on a second area on the membrane. A detection site is disposed on the membrane between the first and second areas in a non-absorbent medium disposed on the membrane between the detection site and the membrane first area is attached by an adhesion with a dry reagent disposed between the medium and the membrane in order to enable mobilization of the reagent by passage of a liquid sample therepast.Type: GrantFiled: December 28, 2001Date of Patent: May 23, 2006Assignee: Varian, Inc.Inventor: Steven S. Bachand
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Patent number: 7045342Abstract: The invention provides an improved test cell for detecting the presence of an analyte in a liquid sample. The device has an elongate casing defining a liquid sample inlet, a reservoir volume, a test volume, and a window through the casing at the test volume. Disposed within the cell is a sample absorbent, a novel biphasic substrate and a reservoir, together capable of transporting an aqueous solution within the casing along a flow path extending from the sample inlet through the test volume and into the reservoir volume. The invention further comprises a method for detecting the presence of an analyte in a liquid sample using the device and a biphasic chromatographic material for carrying out the method.Type: GrantFiled: March 5, 2004Date of Patent: May 16, 2006Assignee: Church & Dwight Co., Inc.Inventors: Albert Nazareth, Mary Beth Boyle, Yea-Shun Cheng
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Patent number: 7037729Abstract: Briefly described, embodiments of this disclosure include structures, methods of forming the structures, and methods of using the structures. One exemplary structure, among others, includes a nanospecies and a porous material. The nanospecies has a first characteristic and a second detectable characteristic. In addition, a second detectable energy is produced corresponding to the second detectable characteristic upon exposure to a first energy. The porous material has the first characteristic and a plurality of pores. The first characteristic causes the nanospecies to interact with the porous material and become disposed in the pores of the porous material.Type: GrantFiled: September 18, 2003Date of Patent: May 2, 2006Assignee: Emory UniversityInventors: Shuming Nie, Xiaohu Gao
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Patent number: 7022529Abstract: Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 ?m in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected.Type: GrantFiled: December 24, 2003Date of Patent: April 4, 2006Assignee: Dade Behring Marburg GmbHInventors: Sharat Singh, John S. Pease, Jacqueline Sadakian, Daniel B. Wagner, Edwin F. Ullman
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Patent number: 7015034Abstract: The subject invention provides new materials and methods for the efficient isolation and purification of stem cells. Specifically, conductive immunopolymers with stem cell specific antibodies can be used to remove stem cells from biological fluids.Type: GrantFiled: October 17, 2001Date of Patent: March 21, 2006Assignee: Morphogenesis, Inc.Inventors: Michael J. P. Lawman, Patricia Lawman
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Patent number: 7011948Abstract: Disclosed are methods for analyzing molecular binding events in which the formation of ligand/antiligand complexes can be directly detected in a mixture without requiring separation of the components of the mixture from each other by measuring bulk properties of the mixture (i.e., properties that have contributions from several or all of the components present in the mixture). Using these techniques, it is possible to screen libraries without labeling either the target antiligand or ligand. The invention also provides a method for determining the strength of ligand/antiligand binding by further analysis of the same signals.Type: GrantFiled: July 17, 2002Date of Patent: March 14, 2006Assignee: MDS SciexInventors: Robert G. Chapman, Pejman Ghanouni, Igor Shmulevich, David Balaban, Joseph A. Heanue, Andrew P. May, Nikolai Sharkov
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Patent number: 6991941Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.Type: GrantFiled: October 17, 2000Date of Patent: January 31, 2006Assignee: BioArray Solutions Ltd.Inventor: Michael Seul
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Patent number: 6991907Abstract: Antibodies and methods are described for the detection and quantitation of cardiac specific troponin I in samples. Cardiac-specific troponin isoforms exist in various forms in the blood, including free and complexed forms. By selecting antibodies that are insensitive and/or sensitive to these various forms, the present invention can provide immunoassays that more accurately reflect the clinical state of an individual. These described antibodies and methods can be used for providing indicators of myocardial infarction and other cardiac pathologies.Type: GrantFiled: October 12, 2000Date of Patent: January 31, 2006Assignee: Biosite, Inc.Inventors: Kenneth F. Buechler, Paul H. McPherson
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Patent number: 6979573Abstract: The present invention provides imprint compositions useful for capturing, isolating, detecting and/or quantifying macromolecules in a sample, methods of making and using the same. Generally, the imprint compositions comprise a matrix material defining an imprint of a template molecule, and the template molecule typically corresponds to a portion of a macromolecule of interest.Type: GrantFiled: June 28, 2001Date of Patent: December 27, 2005Assignee: Aspira Biosystems, Inc.Inventor: Chin-Shiou Huang
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Patent number: 6960457Abstract: This invention provides materials and methods for the site specific attachment of virtually any moiety to a layered silicate surface. The methods involve covalently attaching the moiety to an arginine tag; and contacting the arginine tag with the layered silicate (e.g., mica) surface.Type: GrantFiled: September 3, 1998Date of Patent: November 1, 2005Assignee: Stanford UniversityInventors: James A. Spudich, Steffen Nock, Peter Wagner
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Patent number: 6958245Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled elektrokinetic assembly of particles near surfaces relies on the combination of three functional elements, the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.Type: GrantFiled: November 28, 2001Date of Patent: October 25, 2005Assignee: Bioarray Solutions Ltd.Inventors: Michael Seul, Alice X. Li
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Patent number: 6958131Abstract: The optical sensor contains an optical waveguide (1) with a substrate (104), waveguiding material (105), a cover medium (106) and a waveguide grating structure (101-103). By means of a light source (2), light can be emitted to the waveguide grating structure (101-103) from the substrate side and/or from the cover medium side. (101-103). With means of detection (11), at least two differing light proportions (7-10) radiated from the waveguide (1) can be detected. For carrying out a measurement, the waveguide can be immovably fixed relative to the light source (2) and the means of detection (11). The waveguide grating structure (101-103) itself consists of one or several waveguide grating structure units (101-103), which if so required can be equipped with (bio-)chemo-sensitive layers. The sensor permits the generation of absolute measuring signals.Type: GrantFiled: July 23, 2004Date of Patent: October 25, 2005Assignee: Artificial Sensing Instruments ASI AGInventor: Kurt Tiefenthaler
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Patent number: 6949347Abstract: The present invention is directed to methods and compositions for determining the presence, absence, and/or amounts of one or more membrane-associated analytes in a sample. In accordance with the invention, binding compounds derivatized with releasable molecular tags specifically bind to selected membrane-associated analytes, after which the molecular tags are released upon activation of cleavage moieties, or sensitizers, anchored in the same membrane as the membrane-associated analytes. The released molecular tags are then identified by their distinct separation and detection characteristics.Type: GrantFiled: March 4, 2003Date of Patent: September 27, 2005Assignee: Aclara Biosciences, Inc.Inventors: Sharat Singh, Po-Ying Chan-Hui
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Patent number: 6939678Abstract: Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I an T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed is a system to determine the presence of a troponin form or a group of troponin forms in a sample of whole blood, serum or plasma. Disclosed is a stabilized composition of troponin; the stabilized composition can comprise a stabilized composition of troponin I, wherein the troponin I is oxidized, the troponin I can be unbound or the troponin I can be in a complex. Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays.Type: GrantFiled: July 7, 1999Date of Patent: September 6, 2005Assignee: Biosite, Inc.Inventors: Kenneth F. Buechler, Paul H. McPherson
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Patent number: 6929955Abstract: The present invention relates to an interactive system comprising at least one active surface of plastic from monomers containing at least one structural element derived from a carbon dioxide (A), and at least one substance associated to a linker with at least one structural element (B) capable of establishing a hydrogen bond, and involving an interaction between the structural elements (A) and (B). That interactive system is suitable for presenting and eliminating substances in liquids.Type: GrantFiled: October 14, 1999Date of Patent: August 16, 2005Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., BerlinInventors: Elke Bucha, Götz Nowak
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Patent number: 6924111Abstract: Provided is a microarray substrate. The microarray substrate includes a patterned photoresist film having one or more spot regions therein. The photoresist film can be detached from the substrate.Type: GrantFiled: January 20, 2004Date of Patent: August 2, 2005Assignee: Samsung Electronics Co., Ltd.Inventors: Soo-suk Lee, Sun-hee Kim, Young Choi
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Patent number: 6897073Abstract: Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.Type: GrantFiled: October 27, 2001Date of Patent: May 24, 2005Assignee: Zyomyx, Inc.Inventors: Peter Wagner, Peter Kernen, Hongbo Lu, Huu Tran
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Patent number: 6893816Abstract: An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is applied to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.Type: GrantFiled: April 21, 1998Date of Patent: May 17, 2005Assignee: Houston Advanced Research CenterInventor: Kenneth L. Beattie
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Patent number: 6887701Abstract: The microarrays of the present invention are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.Type: GrantFiled: June 14, 2001Date of Patent: May 3, 2005Assignee: Large Scale Proteomics CorporationInventors: Norman G. Anderson, N. Leigh Anderson
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Patent number: 6872577Abstract: An agglutination assay method for quantitatively determination of an analyte in a liquid sample using particles bearing an anti-analyte. A non-fluid substance which retains the particles while suppressing the diffusion of the particles therein is used as a medium which is to be a place where the agglutination of the particles takes place. Upon analysis, a solation agent is added to the non-fluid substance medium to increase the fluidity of the non-fluid substance, thereby the particles bearing the anti-analyte can diffuse in the medium to cause the agglutination with the analyte. Preferably, the solation agent is added to the non-fluid medium together with the sample. The non-fluid substance medium containing the particle-labeled anti-analyte can be stored with a higher stability in the dry state. A dry analysis element for enabling such analysis method is also provided.Type: GrantFiled: October 22, 2001Date of Patent: March 29, 2005Assignee: Fuji Photo Film Co., Ltd.Inventors: Hiroshi Shinoki, Yoshikazu Amano
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Patent number: 6846635Abstract: Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.Type: GrantFiled: July 28, 2000Date of Patent: January 25, 2005Assignee: Large Scale Proteomics Corp.Inventors: Norman G. Anderson, N. Leigh Anderson, James A. Braatz
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Patent number: 6844162Abstract: A method and kit for monitoring autoantibodies to thyroid stimulating hormone (TSH) receptor in a sample of body fluid, which employs the steps of: (i) incubating TSH receptor with a sample of body fluid; (ii) reacting the incubated sample of body fluid with at least one binding agent which is capable of binding to the TSH receptor in competitive reaction with TSH receptor autoantibodies (TRAb), or in a case where TSH receptor is complexed to labelled antibody, reacting the sample of body fluid with at least one binding agent which can bind to TRAb in such a way as not substantially to interfere with binding of the TRAb to the TSH receptor; and (iii) detecting bound TRAb in the reacted incubated sample of body fluid.Type: GrantFiled: January 31, 2000Date of Patent: January 18, 2005Assignee: RSR LimitedInventors: Bernard Rees Smith, Jane Sanders, Jadwiga Furmaniak
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Patent number: 6844200Abstract: Disclosed is an improvement to a dry assay device for determining the concentration of a first analyte in a sample of body fluid and a second analyte in the same sample of body fluid. The device involves the use of a strip of an absorbent material through which the sample of body fluid flows and wherein the first analyte is determined colorimetrically in a first region of the strip and the second analyte is determined by an immunoassay which takes place in a second region of the strip located downstream from the first region. The improvement involves placing the strip in a hollow casing having a top and a bottom and which is so constructed that when the top and bottom of the casing are mated there is formed a U shaped, body fluid impervious barrier around the first region of the strip to prevent the sample of body fluid from flowing in any direction other than in the direction of the second region of the strip.Type: GrantFiled: August 6, 2001Date of Patent: January 18, 2005Assignee: Bayer CorporationInventor: David A. Brock
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Patent number: 6838552Abstract: The present invention provides a unique approach for the diagnosis and management of infections by Chlamydia species, particularly C. pneumoniae. The invention is based, in part, upon the discovery that a combination of agents directed toward the various stages of the chlamydial life cycle is effective in substantially reducing infection. Products comprising combination of antichlamydial agents, novel compositions and pharmaceutical packs are also described.Type: GrantFiled: November 8, 2000Date of Patent: January 4, 2005Assignee: Vanderbilt UniversityInventors: William M. Mitchell, Charles W. Stratton
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Patent number: 6821790Abstract: A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another step, an additive and particles are added to the sample, wherein the additive substantially binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the particles and wherein the particles substantially bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive, thereby producing a cell-containing network. In a further step, the network is separated from the substantially cell-depleted portion by applying a magnetic force.Type: GrantFiled: September 7, 2001Date of Patent: November 23, 2004Inventors: Vijay Mahant, Byron Doneen
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Patent number: 6780582Abstract: Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.Type: GrantFiled: May 17, 2000Date of Patent: August 24, 2004Assignee: Zyomyx, Inc.Inventors: Peter Wagner, Steffen Nock, Dana Ault-Riche, Christian Itin
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Patent number: 6770489Abstract: The present invention relates to an immunoassay and an apparatus for the same. A method of the present invention comprises following processes: (1) an analyte is labeled to detect antigen-antibody reaction, (2) the magnetic material label is magnetized by a magnetic field, (3) the magnetized magnetic material label detected by a SQUID which detect a magnetic field having right angle to the magnetic field. At the same time, the present invention contains an apparatus to execute the method provided by the present invention. The apparatus comprises a magnetic field generation means that generates a magnetic field to magnetize the labels. The apparatus comprises a SQUID that measures magnetic field.Type: GrantFiled: July 21, 2000Date of Patent: August 3, 2004Assignee: Sumitomo Electric Industries, Ltd.Inventor: Keiji Enpuku
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Patent number: 6767714Abstract: The invention provides an improved test cell for detecting the presence of an analyte in a liquid sample. The device has an elongate casing defining a liquid sample inlet, a reservoir volume, a test volume, and a window through the casing at the test volume. Disposed within the cell is a sample absorbent, a novel biphasic substrate and a reservoir, together capable of transporting an aqueous solution within the casing along a flow path extending from the sample inlet through the test volume and into the reservoir volume. The invention further comprises a method for detecting the presence of an analyte in a liquid sample using the device and a biphasic chromatographic material for carrying out the method.Type: GrantFiled: September 12, 2001Date of Patent: July 27, 2004Assignee: Armkel, LLCInventors: Albert Nazareth, Mary Beth Boyle, Yea-Shun Cheng
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Patent number: 6753190Abstract: The present invention relates to an immunological detection method capable of performing detection of an analyte in a test sample, and a kit used therefor. There is provided an immunological detection method for detecting an analyte by using a water-absorbent substrate in which a capture region is positioned in a given region on a surface thereof, the capture region being immobilized with a first immunochemical component capable of specifically binding to the analyte, wherein the detection signal is amplified and a kit used therefor.Type: GrantFiled: February 29, 2000Date of Patent: June 22, 2004Assignee: Nitto Denko CorporationInventors: Keisaku Okada, Takeshi Saika, Shuji Senda, Kenjiro Mori, Ken Sato
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Patent number: 6720192Abstract: A method for assaying an analyte in a sample. The method comprising the steps of a) contacting the sample with material comprising a receptor which is present in a liposome and which liposome comprises a detectable functionality, said contact occurring under conditions resulting in binding of the receptor to analyte if present before or concomitant with step b, wherein step b) consists of contacting the sample with an immobilised ligand for the receptor said contact occurring under conditions resulting in binding of the receptor to the ligand, with steps a and b being followed by c) separating the resulting immobilised ligand-receptor fraction and the receptor fraction present in solution and d) assaying the detectable functionality of the receptor in a fraction from step c) in a manner known per se for its detection.Type: GrantFiled: May 9, 2001Date of Patent: April 13, 2004Assignee: Merska B.V.Inventors: Gerhard Theodoor Viel, Kornelis Ensing
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Patent number: 6703248Abstract: Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 &mgr;m in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition.Type: GrantFiled: December 15, 1999Date of Patent: March 9, 2004Assignee: Dade Behring Marburg GmbHInventors: Sharat Singh, John S. Pease, Jacqueline Sadakian, Daniel B. Wagner, Edwin F. Ullman
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Patent number: 6703205Abstract: Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.Type: GrantFiled: March 19, 2002Date of Patent: March 9, 2004Assignee: Caliper Technologies Corp.Inventors: Anne R. Kopf-Sill, Andrea W. Chow, Claudia B. Cohen, Steven A. Sundberg, John Wallace Parce
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Patent number: 6696264Abstract: The present invention relates to bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyvinylchloride or polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.Type: GrantFiled: April 17, 2001Date of Patent: February 24, 2004Assignee: Toxin Alert, Inc.Inventors: William T. Bodenhamer, George Jackowski, Eric Davies, Mansel Griffiths
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Patent number: 6696265Abstract: The present invention relates to the substantial elimination of errors attributable to carryover microspheres, doublets, or misclassification of microsphere subsets. The present invention is based upon passing a sufficient minimum number microspheres through the flow analyzer during an assay run.Type: GrantFiled: August 17, 2000Date of Patent: February 24, 2004Assignee: Luminex CorporationInventor: Michael D. Spain
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Patent number: 6692973Abstract: The present invention relates to bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyvinylchloride or polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.Type: GrantFiled: November 28, 2000Date of Patent: February 17, 2004Assignee: Toxin Alert, Inc.Inventors: William T. Bodenhamer, George Jackowski, Eric Davies