Signal Modification Or Steric Inhibition Patents (Class 436/537)
  • Patent number: 10101321
    Abstract: The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample. The wash fluid being substantially refractive index matched to the substrate.
    Type: Grant
    Filed: October 19, 2007
    Date of Patent: October 16, 2018
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Maarten Marinus Johannes Wilhelm Van Herpen, Derk Jan Wilfred Klunder, Hendrik Roelof Stapert
  • Patent number: 9939436
    Abstract: Disclosed are an immunoassay method which can measure an antigen with high sensitivity and accuracy; and a reagent therefor. In the immunoassay method, an antigen-antibody reaction and/or a measurement is(are) carried out in the presence of a polycarboxylic acid type surfactant. The immunoassay reagent for use in the method is characterized by comprising the polycarboxylic acid type surfactant. By employing such a simple means that the polycarboxylic acid type surfactant is allowed to be present in the reaction and/or measurement system, non-specific reactions can be suppressed effectively even in a highly sensitive immunoassay, and an antigen can be measured accurately and specificity can be improved in the immunoassay.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: April 10, 2018
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Mayumi Kano, Ritsuko Tachibana, Masayuki Iizuka
  • Patent number: 9653691
    Abstract: Novel molecules are provided that include a sensitizer group, an acceptor group, and an electron-transfer barrier that suppresses triplet-triplet energy transfer between the sensitizer group and the acceptor group. Organic light emitting devices (OLEDs) that include a layer including these novel molecules are also provided. These devices may be used to provide highly efficient OLEDs with longer operational lifetime.
    Type: Grant
    Filed: December 12, 2012
    Date of Patent: May 16, 2017
    Assignee: Universal Display Corporation
    Inventors: Woo-Young So, Jui-Yi Tsai
  • Patent number: 9459146
    Abstract: A spectroscopic system is provided. In one embodiment, the spectroscopic system comprises a light source adapted to provide a beam of illumination; an optical system adapted to provide the beam of illumination to a sample and receive a spectroscopy signal from the sample and direct the spectroscopy signal to at least one single channel detector, wherein the optical system comprises an adjustable dispersing element for directing one or more spectral features of the spectroscopy signal to the at least one single channel detector; a calibration detector adapted to determine a set point of the adjustable dispersing element; and a source synchronization component adapted to synchronize an operation of the light source and the at least one single channel detector. A method of calibrating a dispersing element of a spectrometer is also provided.
    Type: Grant
    Filed: October 20, 2014
    Date of Patent: October 4, 2016
    Assignee: MKS Technology
    Inventors: Keith Carron, Mark Watson, Shane Buller
  • Patent number: 9297768
    Abstract: Fluorophores or other indicators can be used to label and identify one or more defects in a graphene layer by localizing at the one or more defects and not at other areas of the graphene layer. A substrate having a surface at least partially covered by the graphene layer may be contacted with the fluorophore such that the fluorophore selectively binds with one or more areas of the surface of the underlying substrate exposed by the one or more defects. The one or more defects can be identified by exposing the substrate to radiation. A detected fluorescence response of the fluorophore to the radiation identifies the one or more defects.
    Type: Grant
    Filed: April 18, 2013
    Date of Patent: March 29, 2016
    Assignee: Empire Technology Development LLC
    Inventors: Thomas A. Yager, Seth Adrian Miller
  • Patent number: 8993246
    Abstract: A bioassay employing a first group including a lanthanide ion carrier chelate and a first recognition element, a second group including an antenna ligand and a second recognition element; where the lanthanide ion carrier chelate binds strongly to lanthanide, or the lanthanide ion carrier chelate binds moderately to lanthanide, and an agent complexing the lanthanide ion is additionally employed at a concentration of at least 1 pmol/l. The antenna ligand binds weakly to the lanthanide ion. Analyte recognition by the first recognition element and by the second recognition element results in either chelate complementation and increased fluorescence, or chelate discomplementation and decreased fluorescence.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: March 31, 2015
    Assignee: Oy Arctic Partners AB
    Inventors: Tero Soukka, Urpo Lamminmaki
  • Patent number: 8889424
    Abstract: A device and method for performing a point of care diagnostic test for detecting and quantifying at least one analyte in a biological sample (e.g., a body fluid). A device may include an immunoassay apparatus and a holder with an adjustable variable angle stage for positioning the immunoassay apparatus relative to a light source and a detector device so as to optimize the angle of incidence and angle of radiation to optimize an elastic light scattering signal from the immunoassay apparatus. The elastic light scattering signal may be used to quantify the amount of the analyte(s) of interest present in the sample. The device is based upon elastic light scattering, so the variation in the angle of incidence and angle of reflection are optimized to maximize signal generation due to elastic light scattering.
    Type: Grant
    Filed: September 12, 2012
    Date of Patent: November 18, 2014
    Inventors: Joel R. L. Ehrenkranz, Pamela J. Turbeville, Jeong-Yeol Yoon, David J. You
  • Patent number: 8586316
    Abstract: A single molecule or molecule complex detection method is disclosed in certain aspects, comprising nano- or micro-fluidic channels.
    Type: Grant
    Filed: February 9, 2009
    Date of Patent: November 19, 2013
    Assignees: The Board of Regents of the University of Texas System, The Texas A&M University System
    Inventors: Jun Kameoka, Nan Jing, Mien-chie Hung, Chao-Kai Chou
  • Patent number: 8574925
    Abstract: An object of this invention is to provide a fluorescence analysis method that enables analysis (including imaging) of in vivo substances, etc., using antigen-antibody reactions to be carried out simply, at high sensitivity, and yet continuously and in real time.
    Type: Grant
    Filed: March 18, 2005
    Date of Patent: November 5, 2013
    Assignee: Hamamatsu Photonics K.K.
    Inventor: Masaki Jibu
  • Patent number: 7919018
    Abstract: This invention provides, in one aspect, a procedure to use optically transparent nanocrystalline quantum dots to absorb UV light. This absorption process leads to an energy transfer to a chemically bound and chelated lanthanide ion that may emit light in either the visible spectrum (400-700 nm) or in the near infrared (700-1600 nm). This invention also provides methods for the use of these taggant materials in inks and aerosols used to disperse the taggant.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: April 5, 2011
    Assignee: Voxtel, Inc.
    Inventors: George M. Williams, David M. Schut
  • Patent number: 7820402
    Abstract: The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase.
    Type: Grant
    Filed: September 19, 2005
    Date of Patent: October 26, 2010
    Assignee: Fuji Photo Film Co., Ltd.
    Inventors: Yoshiki Sakaino, Hitomi Ito, Toshihiro Mori, Osamu Seshimoto, Toshihisa Ito, Yoshikazu Amano
  • Patent number: 7750157
    Abstract: The present invention relates to luminescent compounds having a functionalised linker arm, their synthesis and use in bioconjugation and labelling of biomolecules, such as for example nucleosides, nucleotides, nucleic acids (DNA, RNA or PNA) and proteins, as well as their use in the execution of in vitro and in vivo analytic and diagnostic assays.
    Type: Grant
    Filed: June 1, 2005
    Date of Patent: July 6, 2010
    Assignee: Universita' Degli Studi di Torino
    Inventors: Giuseppe Caputo, Roberto Gobetto, Guido Viscardi
  • Patent number: 7749722
    Abstract: A method for measuring an analyte in a whole blood sample comprising the steps of (1) bringing the whole blood sample into contact with a first substance which is carried on an insoluble carrier and specifically binds to the analyte to be measured and a second substance which is labeled with an alkaline phosphatase and specifically binds to the analyte to be measured, and (2) measuring a resulting complex on the basis of an enzyme reaction of the alkaline phosphatase, the measuring step (2) being carried out in the presence of an inhibitor of endogenous alkaline phosphatases, is disclosed.
    Type: Grant
    Filed: December 15, 2005
    Date of Patent: July 6, 2010
    Assignee: Mitsubishi Kagaku Istron, Inc.
    Inventors: Yoshikazu Okamura, Takashi Kurihara, Hiroyuki Yokoi, Junichi Ogawa
  • Patent number: 7723127
    Abstract: The present invention provides a method of performing a competitive assay for the detection and quantification of an analyte over an extended dynamic range. This is achieved by a multi-step sample addition method whereby different concentrations of sample are added at different times during the assay that produces a dose-response curve with multiple windows of detection. This multi-step sample addition method causes the dose-response curve of the composite assay to broaden, dramatically increasing the assay dynamic range.
    Type: Grant
    Filed: March 3, 2005
    Date of Patent: May 25, 2010
    Assignee: Novx Systems Inc.
    Inventors: Samad Talebpour, Stephen W. Leonard
  • Publication number: 20100124790
    Abstract: Provided is a portable optical biosensor measuring apparatus. The portable optical biosensor measuring apparatus includes a light emitting unit emitting a light having a first line width, an optical biosensor receiving an output light from the light emitting unit, and a peak wavelength detector detecting one peak wavelength having a second line width from a light from the optical biosensor. The first line width is greater than the second line width, and the optical biosensor provides the peak wavelength according to an antigen-antibody reaction.
    Type: Application
    Filed: March 3, 2009
    Publication date: May 20, 2010
    Applicant: Electronics and Telecommunications Research Institute
    Inventors: Hyunsung Ko, Chul Huh, Kyung Hyun Kim, Wanjoong Kim, Bong Kyu Kim, Gun Yong Sung, Seon-Hee Park
  • Patent number: 7700298
    Abstract: The invention relates to analytical methods in which the partition of a labeled substance between a liquid and a solid phase is determined. The assays include solid-phase reagents which can be particulate or monolithic such as, for example, a coated tube. Assays of this type are known per se to the person skilled in the art and include immunoassays and immunometric assays.
    Type: Grant
    Filed: February 16, 2006
    Date of Patent: April 20, 2010
    Assignee: Siemens Healthcare Diagnostics Products GmbH
    Inventor: Thomas Wissel
  • Patent number: 7659085
    Abstract: Methods and compositions comprising immunoassays for the detection of antigens and antibodies in a sample are described. In particular, the present invention provides assays that are useful for the rapid and simultaneous detection of multiple different antigens and antibodies. In preferred embodiments, the assays include fluorescent labels of multiple wavelengths or intensities, which are used to label the antigens and antibodies directly and to label beads coated with molecules specific for the antigen or antibody. The detection of a fluorescence shift indicates the presence or identity of the antigen or antibody in the sample.
    Type: Grant
    Filed: September 27, 2002
    Date of Patent: February 9, 2010
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Joseph E. Martinez, George M. Carlone
  • Patent number: 7592187
    Abstract: A method to determine specificity of ligand binding includes comparing a solid phase carrier first extract obtained by pre-treating a sample with a ligand-immobilized solid phase carrier and a solid phase carrier second extract obtained by treating the pretreated sample again with a ligand-immobilized solid phase carrier in terms of the proteins contained therein, and identifying a protein whose content is remarkably decreased in the second extract compared to the first extract, in order to solve 1) the problem of the solubility of subject ligand, 2) the problem of the non-specific protein-denaturing effect of the subject ligand added, and the like, in antagonism experiments in target search using an affinity resin.
    Type: Grant
    Filed: October 15, 2004
    Date of Patent: September 22, 2009
    Assignee: Reverse Proteomics Research Institute Co., Ltd.
    Inventors: Akito Tanaka, Akira Yamazaki
  • Patent number: 7563622
    Abstract: Methods for enhancement in accuracy of immunochemical analysis of heterogeneous biological fluids containing exogenous substances that can interfere in immunochemical analysis for endogenous analytes of interest. According to this method a heterogeneous biological fluid sample is pretreated with an interferant suppression effective amount of a molybdenum coordination complex, so as to reduce manifestation of the presence of said exogenous material under immunoassay conditions. This invention is suitable for the suppression of manifestation of exogenous substances, specifically metabolites of drugs of abuse, during the immunoassay of biological fluids of infants for detection of endogenous substances indicative of a wellness or disease state. This invention also has application for similar suppression exogenous substances in the biological fluid of adults that have been inadvertently exposed to such substances (e.g. secondhand smoke).
    Type: Grant
    Filed: April 23, 2004
    Date of Patent: July 21, 2009
    Inventor: James Matthew Stephens
  • Patent number: 7563587
    Abstract: The present invention relates to a method for analysing a cell sample for cell surface-bound or intracellularly bound analytes by providing an array of immobilised specific binding agents for a set of different ligands, where each ligand is specific to a respective cell surface-bound or intracellularly bound analyte, and using the array and the set of ligands in an inhibition type or a direct type assay format to determine cell surface-bound or intracellularly bound analytes in the cell sample. The invention also relates to assay kits for cell characterization.
    Type: Grant
    Filed: March 31, 2004
    Date of Patent: July 21, 2009
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Robert Karlsson, Pascale Richalet-Secordel
  • Patent number: 7547554
    Abstract: The invention relates to a method and a device for detecting very small quantities of particles. The inventive method is based on a detection of antigen-antibody reaction products and provides a very high detection sensitivity all the way to the femtomolar or attomolar range.
    Type: Grant
    Filed: September 9, 2004
    Date of Patent: June 16, 2009
    Inventor: Constantin Odefey
  • Publication number: 20090053832
    Abstract: A method and kits for assaying a sample of a human or mammalian bodily fluid to simultaneously determine whether one or more of a plurality of drugs and/or metabolites thereof are present in said sample and optionally to perform a semi-quantitative assay for said drug or drugs, comprising: (a) incubating the sample in a competitive assay with a plurality of drug conjugates and a plurality of primary antibodies that bind to the drugs whose presence and optionally whose amount is to be determined, wherein either the plurality of drugs or the plurality of primary antibodies is coupled to microparticles comprising magnetically responsive material, the particles being divided into subsets of particles, each distinguishable from the others by one or more differentiation parameters and by the drug to which they are coupled; (b) incubating the product of step (a) with a liquid medium comprising one or more labeled ligands for the primary antibodies or drug conjugates; (c) magnetically separating microparticles in
    Type: Application
    Filed: August 20, 2007
    Publication date: February 26, 2009
    Inventors: William F. Link, Renato B. Del Rosario, Randy V. Sweet, David L. King
  • Publication number: 20080227221
    Abstract: A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
    Type: Application
    Filed: May 27, 2008
    Publication date: September 18, 2008
    Applicant: DIACHEMIX, LLC
    Inventors: Mohammad Sarwar Nasir, Michael E. Jolley
  • Publication number: 20080206788
    Abstract: Immunoassay reagents, methods and test kits for the specific quantification of vancomycin in a test sample are disclosed. The reagent comprises antibodies prepared with immunogens of FIG. 6 wherein P is an immunogenic carrier material and X is a linking moiety. Also described is the synthesis of labeled reagents of FIG. 8 wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative, and X is a linking moiety.
    Type: Application
    Filed: October 9, 2007
    Publication date: August 28, 2008
    Inventors: Maciej Adamczyk, Elaine M. Brate, Mary M. Perkowitz, Sushil D. Rege
  • Publication number: 20080194044
    Abstract: A lanthanide chelate is linked to a noble metal substrate at a proximity wherein the substrate enhances a fluorescent emission of the lanthanide by at least 20-fold when the chelate is illuminated at an excitation wavelength that induces a transition in the lanthanide that results in the fluorescent emission.
    Type: Application
    Filed: January 24, 2006
    Publication date: August 14, 2008
    Inventors: Gregory W. Faris, Jeanne P. Haushalter
  • Patent number: 7332344
    Abstract: A method of detecting an analyte is described in which an up-converting luminescent species that is excited by absorption of long wavelength light is provided as a donor of energy to an acceptor species that can be bound to, or generated in proximity to, the luminescent species. The analyte either alters the proximity between the luminescent species and the acceptor species or alters the absorbance or luminescence properties of the acceptor species. The luminescent energy donor is excited with long wavelength light and transfers up-converted energy to the proximate acceptor species and this transfer of energy is detected by measurement of appropriate luminescence properties of either the donor or of the acceptor or both.
    Type: Grant
    Filed: February 28, 2002
    Date of Patent: February 19, 2008
    Assignee: Photonic Research Systems Limited
    Inventor: Christopher G. Morgan
  • Patent number: 7241578
    Abstract: The immunoassay method of this invention measures the content of a subject substance in a sample. The method includes the steps of: (a) preparing a mixed solution by mixing the sample and an antibody solution including a first monoclonal antibody and a second monoclonal antibody capable of specifically binding to the subject substance; and (b) measuring an optical property of the mixed solution. The first monoclonal antibody is capable of binding to a first epitope of the subject substance, and the second monoclonal antibody is capable of binding to a second epitope of the subject substance different from the first epitope. Each of the first and second epitopes exists singly in the subject substance.
    Type: Grant
    Filed: May 20, 2003
    Date of Patent: July 10, 2007
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Keiko Yugawa, Nobuyuki Shigetoh, Akihito Kamei, Tatsurou Kawamura
  • Patent number: 7211440
    Abstract: The invention relates to an enhancement solution for an assay technology using lanthanide ions or their chelates as labels and dissociative fluorescence enhancement as a tool for detection, wherein said enhancement solution comprises a ?-diketone of formula I wherein R1 is an aryl, optionally mono- or multi-substituted, and R2 is a straight or branched alkyl chain with 2 to 9 carbon atoms substituted with four or more fluorine atoms. The invention further relates to a bioaffinity assay using lanthanide ions or their chelates as labels and dissociative fluorescence enhancement as a tool for detection comprising the use of said enhancement solution.
    Type: Grant
    Filed: March 8, 2002
    Date of Patent: May 1, 2007
    Assignee: Wallac Oy
    Inventors: Iikka Hemmilä, Kaj Blomberg, Veli-Matti Mukkala, Harri Hakala
  • Patent number: 7144709
    Abstract: A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.
    Type: Grant
    Filed: November 16, 2004
    Date of Patent: December 5, 2006
    Assignee: LifeScan, Inc.
    Inventors: Tianmei Ouyang, Yeung Siu Yu
  • Patent number: 7029856
    Abstract: The invention relates to analytical methods in which the partition of a labeled substance between a liquid and a solid phase is determined. The assays include solid-phase reagents which can be particulate or monolithic such as, for example, a coated tube. Assays of this type are known per se to the person skilled in the art and include immunoassays and immunometric assays.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: April 18, 2006
    Assignee: Dade Behring Marburg GmbH
    Inventor: Thomas Wissel
  • Patent number: 7026166
    Abstract: The present invention relates to the use of fluorogenic or chromogenic dyes as reporter molecules for detecting cell entry by a specific molecule.
    Type: Grant
    Filed: January 21, 2003
    Date of Patent: April 11, 2006
    Assignee: Chiron Corporation
    Inventors: Daniel J. Suich, Ronald N. Zuckermann
  • Patent number: 7011954
    Abstract: A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.
    Type: Grant
    Filed: September 15, 2003
    Date of Patent: March 14, 2006
    Assignee: Lifescan, Inc.
    Inventors: Tianmei Ouyang, Yeung Siu Yu
  • Patent number: 7011955
    Abstract: The invention concerns a method for quantitative or qualitative determination of an analyte or its interaction or reaction kinetics in a system with at least two different phases, comprising the step of taking at least one measurement signal from at least one of the phases, whereby the different phases are present in parallel when taking the signal and whereby each measurement signal is attributed to one of at least two phases. In addition, the invention concerns a sample carrier, in particular for use in the method constituting the invention with one or more wells. The sample carrier is characterized by the fact that at least a portion of the sample carrier at least in the range of one or more wells is coated with fluorescence-quenching material.
    Type: Grant
    Filed: January 27, 2000
    Date of Patent: March 14, 2006
    Assignee: Universitaet Tuebingen
    Inventors: Ivo Stemmler, Andreas Brecht, Gunter Gauglitz, Michael Steinwand
  • Patent number: 6991939
    Abstract: Methods and devices are provided for the trapping, including optical trapping; analysis; and selective manipulation of particles on an optical array. A multi-channel device parcels a light source into many points of light transmitted through an optical array of fibers or conduits, preferably where the individual points of light are individually controllable through a light controlling device. Optical properties of the particles may be determined by interrogation with light focused through the optical array. The particles may be manipulated by immobilizing or releasing specific particles, separating types of particles, etc.
    Type: Grant
    Filed: July 19, 2002
    Date of Patent: January 31, 2006
    Assignee: Tufts University
    Inventors: David R. Walt, Irving L. Weissman, Israel Biran, Jenny Tam
  • Patent number: 6913935
    Abstract: A method for the assay of N samples each containing a compound to be tested, comprises providing N reaction vessels each containing a population of carrier beads and other reagents for performing the assay, where N is at least 2 e.g. 80-4000. Each population of carrier beads is distinguishable from every other population. After adding the samples to the reaction vessels and performing the assays, the contents of all the reaction vessels are mixed and subjected to analysis by flow cytometry. By means of flow cytometry, each carrier bead is rapidly analysed to identify its population and also to determine the presence or concentration or biological activity of the compound to be tested.
    Type: Grant
    Filed: December 3, 1998
    Date of Patent: July 5, 2005
    Assignee: Amersham Biosciences UK Limited
    Inventor: Nicholas Thomas
  • Patent number: 6908769
    Abstract: The present invention concerns new fluorescent dye systems especially for fluorescence resonance energy transfer determinations for example combined with the time-resolved measurement of the resulting fluorescence. The invention also concerns the use of these dyes to label biomolecules and for the homogeneous determination of interactions between biomolecules, for example for detecting an analyte.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: June 21, 2005
    Assignee: Roche Diagnostics GmbH
    Inventors: Daniel Belik, Hans-Peter Josel, Rupert Herrmann, Bernard Koenig, Francis Mueller
  • Patent number: 6887712
    Abstract: A method is disclosed for determining whether a compound binds to a lipoprotein such as LDL or VLDL in a manner which will lower plasma cholesterol. The method provided includes assessing the ability of the compound to form a complex with the lipoprotein, and then determining whether the newly formed complex causes a change in the structure of apoB-100 that results in increased binding affinity to an LDL receptor.
    Type: Grant
    Filed: November 9, 1999
    Date of Patent: May 3, 2005
    Assignee: AtheroGenics, Inc.
    Inventors: Russell M. Medford, Uday Saxena
  • Patent number: 6841392
    Abstract: The present invention relates to bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyvinylchloride or polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.
    Type: Grant
    Filed: August 15, 2001
    Date of Patent: January 11, 2005
    Assignee: Toxin Alert, Inc.
    Inventor: William T. Bodenhamer
  • Patent number: 6753156
    Abstract: The invention relates to a novel homogeneous method of detecting and/or determining the phosphorylating activity of a biological material towards a substrate containing tyrosine and/or serine and/or threonine, and to a kit for carrying out this method.
    Type: Grant
    Filed: March 6, 2000
    Date of Patent: June 22, 2004
    Assignee: Cis Bio International
    Inventors: GĂ©rard Mathis, Eric Trinquet, Marc Preaudat
  • Patent number: 6723567
    Abstract: The present invention relates to a method for the determination of non-, anti-, or pro-apoptotic and necrotic conditions of cells, newly designed vectors coding for marker proteins, cell lines transfected with such vector, and a method to assay the non-, pro- or anti-apoptotic or necrotic activity of test compounds.
    Type: Grant
    Filed: September 19, 2000
    Date of Patent: April 20, 2004
    Assignee: Aponetics Ltd.
    Inventors: Thomas Harr, Alessandro Strebel, Peter Erb, Sinuhe Hahn
  • Patent number: 6716640
    Abstract: The invention concerns the stabilization and amplification of electrochemiluminescence signals in detection methods.
    Type: Grant
    Filed: May 11, 2001
    Date of Patent: April 6, 2004
    Assignee: Roche Diagnostics GmbH
    Inventors: Gabriele Punzmann, Martin Egger, Hans-Peter Josel
  • Patent number: 6682898
    Abstract: This invention provides receptor peptides that have a high affinity for STAT4 and STAT6 polypeptides. Also provided are assays that are useful for identifying compounds that modulate the interaction between STAT4 and STAT6 polypeptides and their respective cellular promoters. The assays are amenable to high throughput screening.
    Type: Grant
    Filed: March 26, 2001
    Date of Patent: January 27, 2004
    Assignee: Tularik Inc.
    Inventors: Pengguang Wu, Judi McKinney
  • Patent number: 6667179
    Abstract: The invention provides methods and compositions for detecting binding or unbinding of a molecule to a substrate. The molecule comprises a luminophore and the substrate comprises a semiconductor which acts as a luminescence quencher to provide distance-dependent quenching of the luminophore. Binding or unbinding of the molecule, which may be covalent or noncovalent, is detected as a decrease or increase, respectively, of the detectable luminescence of the luminophore.
    Type: Grant
    Filed: October 28, 1999
    Date of Patent: December 23, 2003
    Assignee: The Board of Trustees of the University of Illinois
    Inventor: Paul R. Selvin
  • Patent number: 6660532
    Abstract: The invention provides a reagentless assay kit for analyte in a sample comprising a modular affinity assembly including at least one sensor unit comprising a ligand having binding affinity for the analyte (affinity module) operatively associated with a reporter probe (reporter module) responsive to changes in the sensor unit induced by analyte/receptor complex formation by transduction of a characteristic detectable signal. Assays employing the modular assembly are also provided.
    Type: Grant
    Filed: December 16, 1999
    Date of Patent: December 9, 2003
    Assignee: Science & Technology Corporation @ UNM
    Inventors: Gabriel P. Lopez, Larry A. Sklar, Philip Hampton, Leonard Tender, Kimberly Opperman, Emmanuel Rabinovich, Ravinder K. Jain, Juchao Yan
  • Patent number: 6656696
    Abstract: This invention relates to methods and compositions for monitoring the interaction of binding partners as a function of the addition or subtraction of a phosphate group to or from one of the binding partners by a protein kinase or phosphatase.
    Type: Grant
    Filed: February 26, 1999
    Date of Patent: December 2, 2003
    Assignee: Cyclacel
    Inventors: Roger K. Craig, John Colyer
  • Patent number: 6656697
    Abstract: A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.
    Type: Grant
    Filed: February 25, 2000
    Date of Patent: December 2, 2003
    Assignee: Lifescan, Inc.
    Inventors: Tianmei Ouyang, Yeung Siu Yu
  • Patent number: 6645733
    Abstract: A method for assaying specific binding between a fluorophore-labeled probe and an unlabeled target is provided. The method includes detecting a quenching effect on fluorescence emitted by the fluorophore-labeled probe resulting from binding. The method is conducted without separating complexes of the target and probe from the free target and free probe prior to quenching effect detecting, and without providing a signal quenching agent to quench fluorescent light. Preferably, the probe and target are amino acid-containing compounds, such as proteins. The method can be used for a variety of applications, including screening for drug candidates having optimum binding properties, and quantifying and classifying the binding characteristics between peptide-containing compounds. The method is more sensitive than conventional assays, enabling the analysis of minute samples and low affinity binding interactions between receptors and ligands that are below the detection limits of conventional technology.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: November 11, 2003
    Assignee: Ingeneus Corporation
    Inventors: Jasmine I. Daksis, Glen H. Erikson
  • Patent number: 6623982
    Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.
    Type: Grant
    Filed: July 12, 1999
    Date of Patent: September 23, 2003
    Assignee: Immunivest Corporation
    Inventors: Paul A. Liberti, Galla Candra Rao, Leon W. M. M. Terstappen
  • Patent number: 6620627
    Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.
    Type: Grant
    Filed: October 31, 2000
    Date of Patent: September 16, 2003
    Assignee: Immunivest Corporation
    Inventors: Paul A. Liberti, Galla Candra Rao, Leon W. M. M. Terstappen
  • Patent number: 6596546
    Abstract: In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.
    Type: Grant
    Filed: September 22, 1999
    Date of Patent: July 22, 2003
    Inventors: Michael E. Jolley, Klaus H. Nielsen