Involving Immune Complex Formed In Liquid Phase Patents (Class 436/536)
  • Patent number: 10768251
    Abstract: Disclosed is a method for analyzing an NMR spectrum of a lipoprotein-containing sample. The method includes the following steps: a) defining a spectral range to be analyzed of at least 0.5 ppm of an NMR spectrum of a lipoprotein-containing sample; b) fitting a first spectral function of a first individual lipid component and at least one further spectral function of a further individual lipid component into the whole spectral range to be analyzed; and c) determining a concentration or an amount of the first individual lipid component and the further individual lipid component and therefrom a concentration or an amount of the first defined lipoprotein class or lipoprotein subclass in the lipoprotein-containing sample and optionally of each other defined lipoprotein class or lipoprotein subclass based on the fitting operations of step b).
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: September 8, 2020
    Assignee: numares AG
    Inventor: Daniela Baumstark
  • Patent number: 10739346
    Abstract: The present invention relates to a method for diagnosis of a cancer, comprising the steps of (i) determining the level of antibodies against epidermal growth factor receptor (EGFR) in a sample from a subject to be diagnosed, (ii) comparing the determined level in the sample to a control level derived from subjects without cancer; wherein a decreased level in the sample from the subject to be diagnosed as compared to the control level is indicative for cancer in the subject. Furthermore, the invention relates to method of predicting response and outcome of a treatment of a cancer with an inhibitor of EGFR activity.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: August 11, 2020
    Assignee: CELLTREND GMBH
    Inventors: Harald Heidecke, Kai Schulze-Forster
  • Patent number: 10408759
    Abstract: Methods for producing a high resolution image of a sample are provided. In some embodiments, the method includes: detecting first and second sets of spatially-dependent emission signals from a sample labeled with a fluorescent polymeric dye; and producing a high resolution fluorescence image of the sample from the detected first and second sets of spatially-dependent emission signals. In some embodiments, the sample is a cell. Also provided are systems for imaging a sample that include a high resolution light microscope including a light source configured to irradiate a field of view with an excitation light; a photodetector configured to detect an emission signal: and a polarization modulator disposed in the light pathway between the light source and the photodetector; and a sample labelled with a polymeric dye and disposed in the field of view.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: September 10, 2019
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventor: James Ghadiali
  • Patent number: 10389406
    Abstract: One example discloses a near-field device, comprising: a near-field receiver coupled to a near-field receiver antenna and a decoder circuit; wherein the near-field receiver antenna is configured to be capacitively coupled at a first location of a first substance; wherein the near-field receiver antenna is configured to receive a first near-field signal from the first substance through the receiver's capacitive coupling; and wherein the decoder circuit is configured to compare an attribute of the first near-field signal to an attribute of a second near-field signal received from a second substance.
    Type: Grant
    Filed: July 5, 2017
    Date of Patent: August 20, 2019
    Assignee: NXP B.V.
    Inventors: Anthony Kerselaers, Pieter Verschueren, Liesbeth Gommé
  • Patent number: 10132900
    Abstract: In a method and apparatus for magnetic resonance examination of an examination object, in order to determine a substance by execution of a magnetic resonance fingerprinting procedure for examination of an examination object in which a substance is located, a magnetic resonance signal waveform of a voxel of an examination area of the examination object is acquired by a magnetic resonance fingerprinting recording procedure, and a signal comparison of the magnetic resonance signal waveform is made in a computer with a substance signal waveform stored in a database, and the result of the signal comparison is provided as an output from the computer.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: November 20, 2018
    Assignee: Siemens Aktiengesellschaft
    Inventors: David Grodzki, Rene Kartmann
  • Patent number: 10087444
    Abstract: Certain embodiments are directed to methods of identifying neuroblastoma differentiation-inducing compounds or agent and their use in treating neuroblastoma.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: October 2, 2018
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventor: Luqin Du
  • Patent number: 9910045
    Abstract: The present invention relates to a method of determining a protein in a sample and the use of the method for the identification of aptamer target proteins.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: March 6, 2018
    Assignee: RHEINISCHE FRIEDRICH-WILHELMS UNIVERSITAT BONN
    Inventors: Michael Famulok, Gunter Mayer, Jan Vinkenborg
  • Patent number: 9658231
    Abstract: The present disclosure provides compositions and methods for using one or more polypeptide probes to profile an immune response. The polypeptide probe can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for characterizing a cancer based on the detection of one or more antibodies, such as autoantibodies.
    Type: Grant
    Filed: August 10, 2015
    Date of Patent: May 23, 2017
    Assignees: The Regents of the University of Michigan, Armune Biosciences, Inc.
    Inventors: Arul M. Chinnaiyan, Xiaoju Wang, Alex Tsodikov, Jeanne Ohrnberger
  • Patent number: 9476848
    Abstract: Monitoring cell (100) for performing an NMR measurement of a reaction fluid. The monitoring cell (100) has a hollow NMR sample probe (110) for receiving the reaction fluid. Inlet and outlet transport capillaries (112, 123) transport the reaction fluid to and from the sample probe (110). A feed line (306) and return line transport a temperature control fluid to and from the monitoring cell (100). An adapter head (108) couples the transport capillaries (112, 123) to the sample probe (110) and removably connects the sample probe (110) to an adapter section (106). The transport capillaries (112, 123) are positioned within the feed line (306) in parallel to one another. The feed and the return lines (306, 358) are attached to the adapter section (106) such that a reversal of the temperature control fluid stream occurs in the adapter section (106).
    Type: Grant
    Filed: March 16, 2016
    Date of Patent: October 25, 2016
    Assignee: Bruker BioSpin GmbH
    Inventor: Martin Hofmann
  • Patent number: 9320835
    Abstract: Provided are a polymer composition on a substrate and a surface modification method which is non-selective to substrate materials. Chemical vapor deposition polymerization is used to deposit a maleimide-functionalized poly-p-xylylene coating on a substrate. The substrate is readily available to perform a thiol-maleimide coupling reaction under mild conditions so as to modify the surface thereof. Furthermore, through a tailored thiol-terminal molecule, a designer surface can be created via thiol-maleimide coupling on a substrate, and the resulting surface can exhibit various desired biological functions for biotechnological applications. Therefore, this modification technique can be applied to biological fields extensively.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: April 26, 2016
    Assignee: National Taiwan University
    Inventors: Hsien-Yeh Chen, Sheng-Tung Huang, Meng-Yu Tsai
  • Patent number: 9157884
    Abstract: In order to provide a method of electrochemically detecting a target substance, a method of electrochemically detecting an analyte, and a detection set which have a theoretical advantage in the measurement sensitivity obtained by a conventional electrochemical detection method using a working electrode with a trapping substance immobilized, can reuse the working electrode, and can detect an analyte regardless of the size thereof, there is provided a method including: attracting the target substance containing a labeling substance in a liquid sample to a working electrode in which a trapping substance for trapping the target substance containing a labeling substance is not present; and electrochemically detecting the target substance containing a labeling substance.
    Type: Grant
    Filed: August 25, 2011
    Date of Patent: October 13, 2015
    Assignee: SYSMEX CORPORATION
    Inventors: Masayoshi Seike, Nobuyasu Hori, Seigo Suzuki, Shigeki Iwanaga, Hiroya Kirimura
  • Patent number: 9145540
    Abstract: A device for performing a cell study. The device comprises a plate having a plurality of wells, each configured for containing aqueous solution and having a well bottom with a plurality of picowells and a plurality of biosensors each configured for measuring at least one cell characteristic while being in contact with the aqueous solution in a respective the well. The position of each the biosensor in a respective the well is limited by at least one pin.
    Type: Grant
    Filed: February 25, 2010
    Date of Patent: September 29, 2015
    Assignee: Seng Enterprises Ltd.
    Inventors: Mordechai Deutsch, Assaf Deutsch
  • Patent number: 9145472
    Abstract: Described are charge reversible polymers, peptides and their resulting colloidal particles, comprising polymers and peptides having primary and secondary amines that are protected as easily hydrolysable amides. The amides are charge-reversible such that at neutral pH they are negatively charged but become positively charged at pH less than 6 and thus are relatively stable at neutral pH but quickly hydrolyze at pH below 6. Incorporating a drug in a micelle or a polymer comprised of the charge-reversible polymers or peptides provides a drug carrier for delivering the drug preferentially to the solid tumor or other targeted cells.
    Type: Grant
    Filed: February 23, 2011
    Date of Patent: September 29, 2015
    Assignee: Wyoming Research Products Center
    Inventors: Youqing Shen, Peisheng Xu
  • Patent number: 9028771
    Abstract: The invention provides a modified form of saturation assay, which is based on the measurement of a free or unbound labelled reagent fraction (such that there is an increase in signal in the presence of analyte) and employs trapping zones to concentrate said unbound or free labelled fraction to avoid loss of sensitivity. Preferably, the assay is a membrane assay.
    Type: Grant
    Filed: November 9, 2007
    Date of Patent: May 12, 2015
    Assignee: Platform Diagnostics Limited
    Inventors: Douglas Robert Evans, Gerald John Allen, Carolyn Jennifer Ruddell
  • Patent number: 9012136
    Abstract: There is provided mechanisms for the detection of an analyte in a sample. The mechanisms utilize at least a first measurement channel comprising a detection reactant corresponding to the analyte to be detected, and at least a microstructure associated with the first measurement channel. When the mechanisms are in use, the sample is introduced into the first measurement channel and propagated by way of the first measurement channel towards the microstructure. If the analyte is present in the sample, the analyte interacts with the detection reactant to form a networked product, and the microstructure is configured to filter the networked product.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: April 21, 2015
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Daniel J. Solis
  • Patent number: 9005887
    Abstract: There is provided mechanisms for the detection of an analyte in a sample. The mechanisms utilize at least a first measurement channel comprising a detection reactant corresponding to the analyte to be detected, and at least a microstructure associated with the first measurement channel. When the mechanisms are in use, the sample is introduced into the first measurement channel and propagated by way of the first measurement channel towards the microstructure. If the analyte is present in the sample, the analyte interacts with the detection reactant to form a networked product, and the microstructure is configured to filter the networked product.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: April 14, 2015
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Daniel J. Solis
  • Patent number: 8993228
    Abstract: The present invention provides an antibody that specifically binds to envelope protein 2 of HCV of genotype 2a but does not immunologically react with envelope protein 2 of HCV of genotype 1a.
    Type: Grant
    Filed: September 30, 2009
    Date of Patent: March 31, 2015
    Assignees: Toray Industries, Inc., Japan as Represented by Director General of National Institute of Infectious Diseases
    Inventors: Takaji Wakita, Yuko Akazawa, Noriko Nakamura
  • Patent number: 8969024
    Abstract: Compositions and methods comprising proteins that bind specifically to adalimumab are disclosed herein. Adalimumab is a monoclonal antibody specific for the cytokine TNF-? and was developed to treat TNF-? mediated inflammatory diseases. In one aspect of the instant invention, the binding proteins are antibodies directed toward adalimumab. These antibodies, including binding fragments thereof, can be used in a clinical setting as well as for research and development. For example, these anti-adalimumab antibodies can be employed to neutralize adalimumab.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: March 3, 2015
    Assignee: AbbVie Biotechnology Ltd
    Inventors: Zehra Kaymakcalan, Limin Xiong
  • Patent number: 8962264
    Abstract: The present invention provides a method of detecting dermatophyte, which does not require a complicated operation such as an enzyme treatment and a heat treatment. The present invention provides a method of detecting dermatophyte, including a step of extracting a dermatophyte component from a sample with a treatment liquid containing a non-ionic surfactant or a zwitterionic surfactant, and a kit for diagnosing dermatophyte infection, containing a treatment liquid comprising the above surfactant, and an antibody specifically recognizing a dermatophyte component, which are housed in separate containers.
    Type: Grant
    Filed: March 19, 2007
    Date of Patent: February 24, 2015
    Assignees: National University Corporation University of Fukui, JNC Corporation
    Inventor: Sakon Noriki
  • Patent number: 8962260
    Abstract: A lateral flow assay is capable of detecting and differentiating viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In some preferred embodiments, bimodal methods and devices determine if an infection is bacterial and/or viral. A dual use two strip sample analysis device includes a first lateral flow chromatographic test strip to detect MxA and a low level of C-reactive protein and a second lateral flow chromatographic test strip to detect high levels of C-reactive protein. In some preferred embodiments, the sample is a fingerstick blood sample.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: February 24, 2015
    Assignee: Rapid Pathogen Screening, Inc.
    Inventors: Robert P. Sambursky, Robert W. VanDine, Uma Mahesh Babu, Peter Condon
  • Patent number: 8945913
    Abstract: The present invention provides kits, apparatus and methods for determining a biological condition in a mammalian subject, the method includes incubating a specimen from a patient with at least one composition in a kit for a predetermined period of time to form at least one reaction product, when the subject has said biological condition, and receiving an indication of the at least one reaction product responsive to at least one reporter element in the kit thereby providing the indication of the biological condition in the subject.
    Type: Grant
    Filed: June 4, 2014
    Date of Patent: February 3, 2015
    Assignee: LeukoDx Ltd.
    Inventors: Harvey Lee Kasdan, Julien Meissonnier, Yoav Zuta, Bruce Davis, Micha Rosen, Yael Himmel, Yehoshua Broder
  • Patent number: 8940549
    Abstract: The present invention aims to provide an assay reagent and an assay for accurately measuring KL-6, in particular, an assay reagent and an assay for accurately measuring KL-6 in samples containing a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor. KL-6 in samples that contain a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor can be accurately measured using an immunoassay reagent comprising a solution at a pH of 4.0 to 5.5 containing a rheumatoid factor interference inhibitor and a solution of an insoluble carrier on which anti-KL-6 antibodies are immobilized.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: January 27, 2015
    Assignees: Sekisui Medical Co., Ltd., Eidia Co., Ltd.
    Inventors: Junichi Kondou, Mitsuaki Yamamoto, Chie Kaneko
  • Patent number: 8936933
    Abstract: A method of processing a sample may include introducing a sample into a vessel, the vessel having proximal and distal ends, the sample being introduced into the proximal end of the vessel; incubating the sample in the vessel with a substance capable of specific binding to a preselected component of the sample; propelling components of the incubated sample, other than the preselected component, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained; propelling the preselected component toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and mixing the preselected component with a reagent in the distal segment of the vessel.
    Type: Grant
    Filed: May 18, 2010
    Date of Patent: January 20, 2015
    Assignee: IQumm, Inc.
    Inventors: Shuqi Chen, Lingjun Chen
  • Patent number: 8927299
    Abstract: There is disclosed apparatus and a system for effecting testing on a sample, such as for medical testing. The apparatus includes a sample chip (30) provided with at least two chambers (48, 50) within which analyte and a sample to be tested can be located, one chamber being a mixing chamber (48) and the other a detection chamber (50), the latter being provided with a sensor or means to enable sensing of one or more parameters pertaining to the sample. A detector unit (70, 170) includes a slot (76) for holding a sample carrier (30), drive means (94) for moving parts of a sample from the mixing chamber (48) to the detection chamber (50), such as by electromagnetic force, sensing means (60) for sensing the one or more parameters, a diagnostic unit (84) for analyzing the sensed parameters and a display unit (72) for displaying the results of the test to a user. The test unit (70, 170) is preferably handheld, which the sample carrier (30) is preferably in the form of a disposable chip.
    Type: Grant
    Filed: May 26, 2009
    Date of Patent: January 6, 2015
    Assignee: The Secretary of State for Innovation, Universities and Skills of Her Majesty's Britannic Government
    Inventor: Robert Andrew Porter
  • Patent number: 8906637
    Abstract: The present invention provides method and compositions for detecting target molecules present on cells and tissues. In particular, the methods involve adding primary antibodies such as scFv-targeted lactamase that are directed against a target of interest (e.g., cancer markers) to a tissue sample, followed by adding a lactam-containing compound and finally a lactamase reporter system. In some preferred embodiments, the lactamase reporters are fluorescent reporters that bind to the test tissue. In some particularly preferred embodiments, the test tissue contains at least once cancer cell and/or at least one cancer-associated marker.
    Type: Grant
    Filed: September 9, 2005
    Date of Patent: December 9, 2014
    Assignee: Danisco US Inc.
    Inventor: Steven W. Sherwood
  • Patent number: 8895321
    Abstract: Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device. The receptors are selected based on their affinity for classes of known toxic agents. The receptors are freed and the bound and unbound receptors separated based on differential electrokinetic mobilities while they travel to a detection device.
    Type: Grant
    Filed: May 10, 2011
    Date of Patent: November 25, 2014
    Assignee: STC.UNM
    Inventors: Gabriel Lopez, Linnea Ista, Steven RJ Brueck, Aurelio Evangelista Lara, Mangesh Bore
  • Patent number: 8865398
    Abstract: An in vitro method that allows detection of hepatitis C by detecting hepatitis C virus (HCV) core protein and antibodies to HCV core protein (anti-core antibodies) in a single assay is provided. Cross-reactivity is eliminated in the method preferably by utilizing short peptides, each of which has an amino acid sequence that corresponds to an immunodominant region of the native core protein but which does not wholly encompass the epitope bound by the antibodies utilized in the method. The method can be used to detect the presence of HCV in a subject, and/or to determine the suitability of donor blood or blood products for transfusion purposes. Also provided are diagnostic kits for carrying out the method and a process for selecting suitable capture peptides and monoclonal antibodies for use in the combination method.
    Type: Grant
    Filed: August 28, 2007
    Date of Patent: October 21, 2014
    Assignee: Abbott Laboratories
    Inventors: Brian C. Rodgers, Graham J. Burch
  • Patent number: 8846414
    Abstract: The present invention provides for the detection of cardiac markers on a droplet actuator. An aspect provides a method of assaying a cardiac marker in a biological sample from a subject, the method including providing a droplet actuator, loading the biological sample and assay reagents on the droplet actuator, executing droplet operations to create sample droplets from the sample and reagent droplets from the reagents on the droplet actuator, and executing droplet operations using the sample droplets and reagent droplets to produce a detectable signal indicative of the quantity of the cardiac marker in the biological sample. Still other aspects are provided.
    Type: Grant
    Filed: September 29, 2009
    Date of Patent: September 30, 2014
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Arjun Sudarsan, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair, Allen E. Eckhardt
  • Patent number: 8822232
    Abstract: The present invention relates to compositions and methods for the reduction of atherosclerotic plaques and the decrease in the level of total serum cholesterol, triglycerides, serum LDL cholesterol, and serum HDL cholesterol. The present invention also relates to methods for the diagnosis, prevention and treatment of atherosclerosis and mycoplasma associated diseases.
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: September 2, 2014
    Inventor: Maria de Lourdes Higuchi
  • Patent number: 8785210
    Abstract: Test kit for the detection of an analyte in an aqueous solution, including chromatographic test strips for a hapten-antihapten complex and first and second standardized vessels for receiving and positioning test strips, which include first and second hapten-coupled receptors against the analyte dried onto the interior wall for the formation of the hapten-antihapten complex, where a portion of the standardized vessels further include a known amount of analyte embedded in a glass-like layer of trehalose, which are dried onto the interior wall of the control vessel so that they dissolve during reaction of the sample with the hapten-coupled receptors. Through this standardization, analytes in unknown samples may be safely detected by immunochromatography within minutes through a hapten-antihapten complex.
    Type: Grant
    Filed: June 17, 2009
    Date of Patent: July 22, 2014
    Assignee: IfP Privates Institut fur Produktqualitat GmbH
    Inventor: Wolfgang Weber
  • Patent number: 8765391
    Abstract: A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: July 1, 2014
    Assignee: Zyomyx, Inc.
    Inventors: Frank Zaugg, Renee Tobias, Silvia McManus-Munoz, Peter Kernen, Laurence Ruiz-Taylor, Peter Wagner
  • Patent number: 8758772
    Abstract: The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Borrelia antigens. The peptide compositions comprise polypeptide sequences comprising variants in the IR6 domain of the Borrelia VlsE protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Borrelia antigens and the diagnosis of Lyme disease.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: June 24, 2014
    Assignee: Abaxis, Inc.
    Inventors: Rajesh K. Mehra, Kenneth P. Aron, Dennis M. Bleile, Jeremy Walker, Cristina Cuesico
  • Patent number: 8759115
    Abstract: An assay device for performing an assay on a liquid sample using a detection conjugate capable of binding to an antigen and containing a label. The device includes a substrate surface having a sample addition zone, a reaction zone and an absorbing zone, the zones being connected by at least one fluid passage, wherein the device has a first functionality verifying feature located between the sample addition zone and the reaction zone, and a second functionality verifying feature located within the absorbing zone. Both functionality verifying features are capable of undergoing a detectable change when contacted by the sample, in which the assay device further includes at least one alignment verification zone. There is further provided a kit of parts and a method of conducting an assay.
    Type: Grant
    Filed: December 12, 2012
    Date of Patent: June 24, 2014
    Assignee: Johnson & Johnson AB
    Inventors: Per Ove Ohman, Ib Mendel-Hartvig, Tomas Lindstrom
  • Patent number: 8741662
    Abstract: Methods and kits for diagnosis and prognosis using biomarkers comprising albumin-bound protein/peptide complex (ABPPC).
    Type: Grant
    Filed: June 14, 2007
    Date of Patent: June 3, 2014
    Assignee: The Johns Hopkin University
    Inventors: Jennifer E. Van Eyk, Rebekah Lynn Gundry, Robert J. Cotter
  • Patent number: 8716031
    Abstract: Disclosed is a convenient and effective means for quantifying an antigen-specific canine or human IgE.
    Type: Grant
    Filed: May 12, 2009
    Date of Patent: May 6, 2014
    Assignees: RIKEN, Animal Allergy Clinical Laboratories
    Inventors: Kenichi Masuda, Yasuyuki Ishii, Nobutaka Yasuda
  • Patent number: 8709792
    Abstract: A method and device for detecting analytes in a test sample. Embodiments include methods for quantitatively detecting analytes within a range of concentrations. In an embodiment the method includes a lateral flow test strip with multiple test areas for capturing a labeled receptor to provide a detectable signal.
    Type: Grant
    Filed: July 23, 2010
    Date of Patent: April 29, 2014
    Assignee: Charm Sciences, Inc.
    Inventors: Steven J. Saul, Mark E. Tess, Robert J. Markovsky
  • Patent number: 8685662
    Abstract: The present invention pertains to a method for in vitro prognosticating and/or diagnosing cerebral cerebral malaria, wherein said method comprises a step of detecting non-erythroid spectrin or fragments thereof, and/or antibodies directed against non-erythroid spectrin, in a biological sample. Reagents and kits for performing this method are also disclosed.
    Type: Grant
    Filed: March 30, 2007
    Date of Patent: April 1, 2014
    Assignees: Institut Pasteur, Universite Pierre et Marie Curie (Paris 6)
    Inventors: Sylviane Pied, Vincent Guiyedi, Pierre-André Cazenave, Maryvonne Kombila, Youri Chanseaud
  • Patent number: 8669047
    Abstract: The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: March 11, 2014
    Assignee: Theranos, Inc.
    Inventors: Elizabeth A. Holmes, Ian Gibbons
  • Patent number: 8647578
    Abstract: Device to detect at least an analyte, comprising a transparent substrate (2), having a first surface (3) with which a light source (7) is associated, and a second surface (4) on which a plurality of biological protein probes (12) are disposed, a layer (6) of polymer being interposed between said second surface (4) and said biological protein probes (12). A marker (fluorophore) is associated with said analyte, having determinate characteristics of fluorescence and/or phosphorescence correlated to the emission wavelength of the light source (7). Said light source (7) is suitable to emit a light radiation in a range of wavelengths equal to 400-550 nm, inside which range the absorption peak of said marker (fluorophore) used is comprised. The value of the distance (“s”) between the wavelength corresponding to the absorption peak of the marker (fluorophore) and the wavelength corresponding to the emission peak of fluorescence (phosphorescence) is comprised between 25 and 150 nm.
    Type: Grant
    Filed: October 20, 2010
    Date of Patent: February 11, 2014
    Assignee: OR-EL Organska Elektronika D.O.O.
    Inventor: Patrizia Melpignano
  • Patent number: 8628916
    Abstract: A method for isolating Hepatitis A virus or Spring viremia of Carp virus. A virus probe is prepared by linking a magnetic bead-conjugated Protein G with an anti-HAV (Hepatitis A Virus) antibody or an anti-rhabdovirus antibody. The virus probe is contacted with a sample to be analyzed to form a virus probe-virus complex. The virus probe-virus complex is then isolated. It may specifically isolate Hepatitis A virus or Spring viremia of Carp virus from a sample mixed viruses.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: January 14, 2014
    Assignee: Industry Foundation of Chonnam National University
    Inventors: Du Woon Kim, Hee Min Lee, Se Young Cho, Sang Mu Ko, Kyung Seo Oh, Joseph Kwon, Jong Soon Choi
  • Patent number: 8609356
    Abstract: The invention relates to an in vitro method for detecting pulmonary arterial hypertension (PAHT), or the risk of developing PAHT, which includes determining the presence and/or amount of anti-tenascin C antibodies in a biological sample from a patient.
    Type: Grant
    Filed: April 10, 2009
    Date of Patent: December 17, 2013
    Assignees: Assistance Publique-Hopitaux de Paris, Universite Paris Descartes, Universite Paris-Sud 11
    Inventors: Luc Mouthon, Marc Humbert, Mathieu Tamby
  • Patent number: 8603758
    Abstract: A method of detecting prostate tumorigenesis in a subject, the method including the steps of (a) obtaining a sample from the prostate of the human subject, (b) detecting quantitatively or semi-quantitatively in the sample a level of expression for PKC-? and (c) comparing the expression level in (b) to a level of expression in a normal control, wherein overexpression of PKC-?, with respect to the control, indicates the presence of prostate cancer in the subject. The present invention is based upon the discovery that PKC-? levels are elevated during prostate tumorigenesis. Furthermore, the proliferation rate of the tumor correlates with the level of PKC-?. The invention also provides methods of treating prostate cancer by administering to the subject a compound that inhibits the expression of PKC-?. The compound can be a small interfering RNA (siRNA) molecule.
    Type: Grant
    Filed: June 25, 2012
    Date of Patent: December 10, 2013
    Assignees: University of South Florida, The United States of America as represented by the Department of Veterans Affairs
    Inventors: Mildred Acevedo-Duncan, Hla Y. Win, Raoul Salup
  • Patent number: 8586349
    Abstract: The present invention concerns a method for the detection of an analyte in a droplet of fluid comprising the steps of providing a solid phase with a hydrophobic surface comprising at least one capture zone on which at least one binding agent with affinity for the analyte is immobilized, applying said droplet to the surface of said solid phase, applying a force that makes the droplet travel along the surface of said solid phase along a predetermined path thereby allowing the droplet to repeatedly contact said binding agent on the capture zone, applying conditions wherein said analyte is allowed to bind to said binding agent and detecting a complex of analyte and binding reagent at the position of the capture zone. The effect of a moving droplet is that the reactants in the droplet are well mixed which eliminates the risk of diffusion limitation. Such advantageous mixing is not obtained with conventional assays in which the surface is continuously exposed to the liquid.
    Type: Grant
    Filed: August 6, 2001
    Date of Patent: November 19, 2013
    Assignee: Biomerieux B.V.
    Inventors: Thomas Augustinus Maria Beumer, Petrus Franciscus Hendrikus M. Verheijden, Hendrik Sibolt van Damme
  • Patent number: 8586387
    Abstract: A method of performing a bioassay comprising activating capsules containing a signal precursor that is hydrolysable from a latent form in which substantially no signal is generated to a form in which it is able to generate a detectable signal, said activating comprising treating said capsules with heat and with an acid or a base catalysing solution, the combination of said heat and the pH of the catalysing solution being such as to hydrolyse said precursor to the form in which it is able to generate a detectable signal.
    Type: Grant
    Filed: August 30, 2011
    Date of Patent: November 19, 2013
    Assignee: Supernova Diagnostics, Inc.
    Inventors: Neil J. Campbell, Keith Edward Moravick, Joseph D. Penniman, Bruce J. Richardson
  • Patent number: 8580929
    Abstract: The present invention concerns a novel retinoic acid regulated gene whose expression product displays useful morphogenic/mitogenic properties. The present invention further concerns an isolated nucleic acid of SEQ ID NO:1 encoding a retinoic acid regulated expression product having an amino acid sequence of SEQ ID NO:2.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: November 12, 2013
    Assignee: The Hong Kong University of Science and Technology
    Inventors: Nancy Y. Ip, William M. W. Cheung
  • Patent number: 8569076
    Abstract: A method and system for performing a serological agglutination assay in a liquid sample. The system provides a simple method for creating an in-situ sample/reagent admixture within a sample analysis chamber without the use of any precision fluid-handling components.
    Type: Grant
    Filed: April 2, 2009
    Date of Patent: October 29, 2013
    Assignee: Abbott Point of Care, Inc.
    Inventors: Stephen C. Wardlaw, Robert A. Levine
  • Patent number: 8563327
    Abstract: The present invention provides, as a novel diagnosis marker for type 1 diabetes mellitus, a type 1 diabetes mellitus diagnostic composition comprising alanyl-tRNA synthetase, glycyl-tRNA synthetase, asparaginyl-tRNA synthetase, or tryptophanyl-tRNA synthetase, a diagnostic kit comprising the same, and a diagnostic method using the same. The composition, the kit, and the method, according to the present invention, may be used for early diagnosis and confirmed diagnosis of type 1 diabetes mellitus because type 1 diabetes mellitus can be easily diagnosed from a patient sample.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: October 22, 2013
    Assignees: Seoul National University Hospital, SNU R&DB Foundation
    Inventors: Sang Gyu Park, Kyong Soo Park, Sunghoon Kim
  • Patent number: 8541242
    Abstract: The present invention provides assays and devices for detection of substances in liquid samples. The assays and devices utilize passive diffusion between a porous material and a porous membrane containing a specific binding pair member to enable detection of the substance of interest.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: September 24, 2013
    Assignee: Techlab, Inc.
    Inventors: James H. Boone, David M. Lyerly, Tracy D. Wilkins
  • Patent number: 8535951
    Abstract: The present disclosure provides a reagent for detection of analyte in a sample and process of preparation of the reagent. The reagent comprises heparin and substrate coated with the analyte-counterpart. The analyte-counterpart of the reagent is capable of binding to said analyte. The present disclosure further provides a process for detection of an analyte in a sample by contacting said sample with said reagent and detecting formation of analyte-analyte counterpart complex. The present disclosure provides a kit for detection of an analyte in a sample.
    Type: Grant
    Filed: March 13, 2008
    Date of Patent: September 17, 2013
    Assignees: Director General, Defense Research & Development Organisation, Indian Institute of Science
    Inventors: Ganga Prasad Rai, Gauri Shanker Agarwal, Samuel Merwyn Packia Raj, Krishnamurthy Sekhar, Ajay Kumar Sood, Ajay Singh Negi
  • Patent number: 8530244
    Abstract: Ultrafine particles are provided having a core region that has a signal amplifying molecule and a shell region that surrounds the core region. The shell region has at least one antibody affixed to its surface that is specific for at least one antigen. Alternatively, the ultrafine particles may entrap the signal amplifying molecule within its matrix and may also have antibodies affixed to its surface for molecular recognition. Ultrafine particles are also provided having a matrix component that includes a signal amplifying molecule and at least one antibody specific for the antigen or biomaterial. The ultrafine particles of the present disclosure may be used in assays for the detection, including quantification, of one or more antigens present in a biological sample.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: September 10, 2013
    Assignee: Terapia Celular, L'N, Inc.
    Inventors: Gustavo Larsen, Raffett Velarde Ortiz