Absorbent Column, Particles Or Resin Strip Patents (Class 436/541)
  • Patent number: 10344048
    Abstract: Chemical entities that are novel compounds, pharmaceutical compositions and methods of treatment of cancer are described.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: July 9, 2019
    Assignee: NEUPHARMA, INC.
    Inventor: Xiangping Qian
  • Patent number: 9212203
    Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: December 15, 2015
    Assignee: BIO-RAD LABORATORIES, INC.
    Inventors: Larry J. Cummings, Mark A. Snyder
  • Patent number: 8999729
    Abstract: A device for conducting an agglutination assay comprising several reaction vessels, each reaction vessel comprising an upper chamber having an opening for accepting reactants and/or a sample; and a lower chamber comprising an end in communication with the upper chamber for receiving fluids from the upper chamber, a closed end opposite to the end, and a matrix for separating agglutinates from non-agglutinates; wherein the device further comprises a rotating support able to rotate around an axis and holding pivotally the reaction vessels in a way to allow the reaction vessels to pivot about an axis essentially perpendicular to the rotation axis of the support when the latter is rotated, such that the fluids remain in the upper chamber when the support is not rotated, and can flow from the upper chamber to the lower chamber and into the matrix when the support is rotated.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: April 7, 2015
    Assignee: Symbion Medical Systems Sarl
    Inventors: Isabelle Delafosse, Fabrice Ummel, Antonio Yanez
  • Patent number: 8951807
    Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
    Type: Grant
    Filed: January 13, 2011
    Date of Patent: February 10, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Larry J. Cummings, Mark A. Snyder
  • Patent number: 8933206
    Abstract: The present invention provides a new method for isolation and/or purification of immunoglobulins from a solution containing one or more immunoglobulins using a solid phase matrix represented by the formula: M-SP-L, wherein M is designates a matrix backbone, SP designates a spacer and L designates a substituted benzimidazole ligand.
    Type: Grant
    Filed: January 8, 2008
    Date of Patent: January 13, 2015
    Assignee: Millipore (U.K.) Limited
    Inventors: Gordon R. Duffin, Victoria Jane Dolan, Katherine Louise Angus, Andrew Lyddiatt
  • Patent number: 8921113
    Abstract: A buffer kit includes a first eluent and second eluent. The first eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The second eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The first eluent solution has a first pH and the second eluent solution has a second pH where the first pH and second pH are different values. The buffer kit provides a linear pH gradient that forms an approximately straight line from at least the first pH to the second pH.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: December 30, 2014
    Assignee: Dionex Corporation
    Inventors: Shanhua Lin, Christopher A. Pohl
  • Patent number: 8895709
    Abstract: Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
    Type: Grant
    Filed: October 20, 2009
    Date of Patent: November 25, 2014
    Assignee: AbbVie Inc.
    Inventors: Robert K. Hickman, Qing Huang, Cheryl L. Weed, Scott T. Ennis, Barbara Perilli-Palmer, Min Wan
  • Patent number: 8895710
    Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
    Type: Grant
    Filed: January 4, 2011
    Date of Patent: November 25, 2014
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J. Johansson, Jean-Luc Maloisel
  • Patent number: 8802449
    Abstract: An objective of the present invention is to provide immunoassay microchips in which microstructures of beads having a sufficient reaction area were constructed within microchannels while suppressing flow path resistance, and to provide simple and highly-sensitive immunoassay methods for microsamples. The objective was achieved by immunoassay microchips comprising microchannels with microstructures arranged in at least a portion of the microchannels, the microstructures retaining microbeads uniformly dispersed in photo-cured hydrophilic resins, and the microbeads having a primary antibody immobilized on their surfaces, and by immunoassay methods using the microchips.
    Type: Grant
    Filed: December 25, 2006
    Date of Patent: August 12, 2014
    Assignees: Institute of Microchemical Technology, Kansai Paint Co., Ltd.
    Inventors: Masaya Kakuta, Manabu Tokeshi, Hitoshi Izumida, Takahide Takadera, Kenji Miyagawa
  • Patent number: 8802448
    Abstract: Substrates comprising a solid support, a ligand, and a linker comprising at least one C, O, N, or S atom covalently connecting the solid support to the ligand, are disclosed, along with methods of using and making the substrates, and devices including the substrates.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: August 12, 2014
    Assignee: Pall Corporation
    Inventors: Hassan Ait-Haddou, Frank Onyemauwa, Jonathan Haigh, Samuel Nochumson
  • Patent number: 8785210
    Abstract: Test kit for the detection of an analyte in an aqueous solution, including chromatographic test strips for a hapten-antihapten complex and first and second standardized vessels for receiving and positioning test strips, which include first and second hapten-coupled receptors against the analyte dried onto the interior wall for the formation of the hapten-antihapten complex, where a portion of the standardized vessels further include a known amount of analyte embedded in a glass-like layer of trehalose, which are dried onto the interior wall of the control vessel so that they dissolve during reaction of the sample with the hapten-coupled receptors. Through this standardization, analytes in unknown samples may be safely detected by immunochromatography within minutes through a hapten-antihapten complex.
    Type: Grant
    Filed: June 17, 2009
    Date of Patent: July 22, 2014
    Assignee: IfP Privates Institut fur Produktqualitat GmbH
    Inventor: Wolfgang Weber
  • Patent number: 8778613
    Abstract: A resin which is prepared by polymerizing a monomer component incorporating a hydrophilic spacer, and a ligand-immobilized solid phase carrier obtained by immobilizing a ligand to the resin, are capable of reducing the non-specific adsorption of substances, other than the target molecule for the ligand, which mingle in the sample, to the resin and/or the ligand. Therefore, target molecule search, identification and the like with less noise are enabled.
    Type: Grant
    Filed: October 15, 2004
    Date of Patent: July 15, 2014
    Assignee: Reverse Proteomics Research Institute Co., Ltd.
    Inventors: Akito Tanaka, Takaaki Shiyama, Akira Yamazaki
  • Patent number: 8741662
    Abstract: Methods and kits for diagnosis and prognosis using biomarkers comprising albumin-bound protein/peptide complex (ABPPC).
    Type: Grant
    Filed: June 14, 2007
    Date of Patent: June 3, 2014
    Assignee: The Johns Hopkin University
    Inventors: Jennifer E. Van Eyk, Rebekah Lynn Gundry, Robert J. Cotter
  • Patent number: 8709792
    Abstract: A method and device for detecting analytes in a test sample. Embodiments include methods for quantitatively detecting analytes within a range of concentrations. In an embodiment the method includes a lateral flow test strip with multiple test areas for capturing a labeled receptor to provide a detectable signal.
    Type: Grant
    Filed: July 23, 2010
    Date of Patent: April 29, 2014
    Assignee: Charm Sciences, Inc.
    Inventors: Steven J. Saul, Mark E. Tess, Robert J. Markovsky
  • Patent number: 8598325
    Abstract: There is described a coated solid phase microextraction (SPME) fiber for use in direct immersion SPME of a food matrix that includes carbohydrates. The coated SPME fiber includes a SPME fiber for absorbing a small molecule from the food matrix; and a protective coating which has a surface that is substantially uniform and substantially smooth, the protective coating reducing adsorption of the carbohydrates onto the SPME fiber and allowing the SPME fiber to extract the small molecule from the food matrix. A process for producing the coated SPME fiber and a method of performing solid phase microextraction are also described.
    Type: Grant
    Filed: March 5, 2012
    Date of Patent: December 3, 2013
    Inventor: Janusz B. Pawliszyn
  • Patent number: 8563330
    Abstract: The invention provides a device and method for the rapid identification of patients suspected of having thalassemia. The invention provides a test strip for the aqueous detection of thalassemia related proteins in whole blood. The test strip includes antibodies specific to the gamma 4, (?4) protein and provides easy visual discrimination between a positive result and a negative result. The invention can be used in remote or clinical settings.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: October 22, 2013
    Assignee: National Science and Technology Development Agency
    Inventors: Watchara Kasinrerk, Chatchai Tayapiwatana, Thanusak Tatu, Suthat Fucharoen, Sawitree Chiampanichayakul
  • Patent number: 8557590
    Abstract: A method for measuring glycated hemoglobin includes hemolyzing a blood sample with a hemolysate; reacting the hemolyzed blood sample with bead conjugates in which beads are conjugated with glycated hemoglobin binding materials; measuring the amount of total hemoglobin in the reacted blood sample; isolating normal hemoglobin from the glycated hemoglobin conjugated with the bead conjugates; measuring the amount of glycated hemoglobin isolated from the normal hemoglobin; and determining the percentage of the glycated hemoglobin in the blood sample on the basis of the measured amounts of total hemoglobin and glycated hemoglobin. The isolation of normal hemoglobin is performed by absorbing the normal hemoglobin using an absorption pad that is a porous pad having pores, each of which has a size greater than the size of the normal hemoglobin and smaller than the size of the bead.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: October 15, 2013
    Assignee: Infopia Co., Ltd.
    Inventors: Byeong-Woo Bae, Sung-Dong Lee, Hyong-Soo Kim
  • Patent number: 8491904
    Abstract: Disclosed herein are methods for the isolation and purification of anti-IL-13 antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography (e.g., Protein A affinity chromatography), ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
    Type: Grant
    Filed: October 20, 2010
    Date of Patent: July 23, 2013
    Assignee: Abbvie Inc.
    Inventor: Robert K. Hickman
  • Patent number: 8394933
    Abstract: It is an object of the present invention to provide: a protein refolding column filler, which is effective for the refolding, namely, the activation of the function, of an inactive protein with an as yet unformed higher order structure produced in Escherichia coli or the like, or a protein whose conformation has been changed due to a certain cause and which has become inactivated; and a column filled with the aforementioned column filler. The present invention provides a protein refolding column filler, which comprises zeolite with BEA structure (Zeolite Beta) that is granulated into a particle state.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: March 12, 2013
    Assignee: FUJIFILM Corporation
    Inventors: Masayuki Kawakami, Tatsuo Tsunoda, Hideaki Togashi, Takayuki Nara, Shun-ichi Matsuura, Chisato Sekikawa, Akiko Kawai, Akiyoshi Kawata, Fujio Mizukami, Kengo Sakaguchi
  • Patent number: 8329417
    Abstract: Methods that permit the rapid release of one or more analytes from head or body hair or other keratinized structures of an individual (who may previously have ingested one or more of the analytes) are provided. The methods can include contacting the keratinized structure with a reducing agent but not with a proteolytic agent. The methods can further include identification and quantification of the one or more analytes by known analytical techniques such as immunoassays. The described methods do not damage the analyte and do not cause harmful effects on a subsequently-used analyte detection probe (e.g., an antibody).
    Type: Grant
    Filed: December 23, 2011
    Date of Patent: December 11, 2012
    Assignee: Psychemedics Corporation
    Inventors: Virginia Hill, Mohammad Atefi, Michael I. Schaffer
  • Patent number: 8183056
    Abstract: A method for determining the risk of an individual of suffering from inflammation, opportunistic infection or disruption of immunoglobulin metabolism, comprising (a) determining the level of fragmentation or modification of Fc function of immunoglobulins in a sample taken from the individual and (b) determining thereby the risk of inflammation, impaired immune response or opportunistic infection. The invention also provides the use of a trypsin inhibitor in the manufacture of a medicament for use in the treatment or prevention of a disorder associated with elevated trypsin activity which is correlated with IgG fragmentation or modification.
    Type: Grant
    Filed: October 15, 2008
    Date of Patent: May 22, 2012
    Inventor: Ivan Mikhailovich Petyaev
  • Patent number: 8129132
    Abstract: A method of screening a sample of body fluid obtained from an animal subject for analyte autoantibodies reactive with one or more antigenic molecules selected from pancreatic islet cell antigenic molecules and insulin, or one or more variants, analogues, derivatives or fragments thereof, and a kit for use in such a method.
    Type: Grant
    Filed: November 26, 2002
    Date of Patent: March 6, 2012
    Assignee: RSR Limited
    Inventors: Bernard Rees Smith, Jadwiga Furmaniak, Michael Powell
  • Patent number: 8093057
    Abstract: The present disclosure provides a device for simultaneously detecting hemoglobin and glycohemoglobin in blood. The device includes a lateral flow assay strip, a laser-induced epifluorescence detection device, and an LED detection device. The present disclosure further provides a method for simultaneously quantifying hemoglobin and glycohemoglobin in blood by an immunological method using the same.
    Type: Grant
    Filed: August 5, 2010
    Date of Patent: January 10, 2012
    Assignee: Boditechmed Inc.
    Inventors: Eui Yul Choi, Kie Bong Nahm, Jae Hoon Kim, Dong Seok Jeong, Sang Yeol Park, Joung Dae Moon, Jin Ha Jung, Young Min Kim, So Young Jung, Ae Kyung Park, Byeong Chul Kim, Sung Joong Kim
  • Patent number: 8084215
    Abstract: Methods that permit the rapid release of one or more analytes from head or body hair or other keratinized structures of an individual (who may previously have ingested one or more of the analytes) are provided. The methods can include contacting the keratinized structure with a reducing agent but not with a proteolytic agent. The methods can further include identification and quantification of the one or more analytes by known analytical techniques such as immunoassays. The described methods do not damage the analyte and do not cause harmful effects on a subsequently-used analyte detection probe (e.g., an antibody).
    Type: Grant
    Filed: April 29, 2008
    Date of Patent: December 27, 2011
    Assignee: Psychemedics Corporation
    Inventors: Virginia Hill, Mohammad Atefi, Michael I. Schaffer
  • Patent number: 8076126
    Abstract: A plurality of individual single column test elements are provided for use in a clinical testing apparatus. Each test element is defined by a single test column that includes a quantity of a test material, such as gel material or a bead matrix, including a cover strip used to access the contents of the test column. Individual test elements can be stored, retained and dispensed for testing patient samples.
    Type: Grant
    Filed: July 18, 2008
    Date of Patent: December 13, 2011
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Raymond F. Jakubowicz, Mark Sawczuk
  • Patent number: 7935541
    Abstract: An object of the present invention is to overcome the problem of nonspecific adsorption of a labeled antibody on the detection site and to provide an immunochromatography method by which the false positive signal can be suppressed, and wherein the detection sensitivity is high and the highly reliable measurement can be performed.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: May 3, 2011
    Assignee: Fujifilm Corporation
    Inventor: Hiroyuki Chiku
  • Patent number: 7838306
    Abstract: An adsorbent for whole blood in the form of essentially spherical non-aggregated particles. The adsorbent comprises a porous carrier material having an average pore size of ?1.5 ?m, whereby a maximum of 50% of the pore volume may be in pores having a pore size of >1.5 ?m, whereby the outer surfaces of the porous carrier material have at least one surface modification M1 so that the outer surface essentially does not interact with blood cells, and the inner surfaces of the porous carrier material, e.g., the surfaces of the pores of the porous carrier material, have at least one surface modification M2 which interacts with substances present in blood.
    Type: Grant
    Filed: September 14, 2001
    Date of Patent: November 23, 2010
    Assignee: Fresenius Medical Care Deutschland GmbH
    Inventors: Hanno Baumann, Franz-Josef Gerner, Michael Hoffmann, Roland Horres, Andreas Kokott, Hans-Peter Leinenbach, Gunter Mathar, Wolfgang Metzger, Veit Otto, Walter Ruger, Martin Schimmel
  • Patent number: 7824873
    Abstract: A blood crossmatching apparatus, kit and methods for testing the compatibility of mammals for blood transfusion. Particulate layers in the apparatus allow nonagglutinated red blood cells to permeate through, while agglutinated red blood cells cannot. The apparatus also has a density solution. The density solution separates white blood cells from red blood cells in the whole blood when centrifuged, without lysing the red blood cells. Thus, the apparatus can be used to test whole blood.
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: November 2, 2010
    Inventor: Anne S. Hale
  • Patent number: 7824876
    Abstract: A bioaffinity assay for quantitative determination in a sample of free PAPP-A, defined as the pregnancy associated plasma protein A (PAPP-A) that is not complexed to the proform of major basic protein (proMBP), where free PAPP-A is determined either i) as a calculated difference between measured total PAPP-A and measured PAPP-A complexed to proMBP, or ii) by a direct bioaffinity assay measuring only free PAPP-A. Also disclosed is a method for diagnosing an acute coronary syndrome in a person by using as marker either free PAPP-A as such or a ratio free PAPP-A/total PAPP-A, free PAPP-A/PAPP-A complexed to proMBP, or PAPP-A complexed to proMBP/total PAPP-A.
    Type: Grant
    Filed: January 19, 2005
    Date of Patent: November 2, 2010
    Assignee: Turun Yliopisto
    Inventors: Qiu-Ping Qin, Kim Pettersson
  • Patent number: 7790475
    Abstract: The present invention is directed to apparatuses and methods for reducing the content of albumin in serum, plasma and/or blood samples. The apparatuses comprise an insoluble support with a ligand attached thereto. According to certain embodiments of the invention, the ligand is a bromosulfophthalein, Cibacron Blue or Warfarin ligand or salts or esters thereof. Also provided are methods of making the apparatus, as well as kits and albumin-depleted samples produced by the methods of the present invention.
    Type: Grant
    Filed: August 19, 2004
    Date of Patent: September 7, 2010
    Assignee: Becton, Dickinson and Company
    Inventor: William Galbraith
  • Patent number: 7785898
    Abstract: The present invention provides methods for identifying and/or enriching fetal cells from maternal blood, using as fetal cell markers the antibodies that the mother produces against paternally inherited fetal antigens. The fetal cell-maternal antibody complexes are identified and isolated using labelled agents that bind to the maternal antibodies. The present invention also provides fetal cells, isolated by use of said maternal antibodies, as a source of fetal DNA for prenatal genetic diagnosis of the fetus.
    Type: Grant
    Filed: May 30, 2003
    Date of Patent: August 31, 2010
    Assignee: Genetic Technologies Limited
    Inventor: Ralph M. Bohmer
  • Patent number: 7771928
    Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.
    Type: Grant
    Filed: August 28, 2002
    Date of Patent: August 10, 2010
    Assignee: Fujirebio Inc.
    Inventors: Akira Hasegawa, Yoshihiro Ashihara, Mitsuo Isomura
  • Patent number: 7741050
    Abstract: The invention relates to a method for detecting disease-associated autoantibodies, which recognize extracellular structures of G protein-coupled receptors, and to the use of peptides, which comprise these loops or fragments thereof, for treating autoimmune diseases.
    Type: Grant
    Filed: November 28, 2003
    Date of Patent: June 22, 2010
    Assignee: Max-Delbrueck-Centrum fuer Molkulare Medizin
    Inventor: Gerd Wallukat
  • Patent number: 7718444
    Abstract: This invention provides a simple detection method with excellent sensitivity and specificity, which allows prompt detection of an analyte by allowing a labeled reagent to effectively react with the analyte in the process of treating an analyte-containing specimen, such as during removal of impurities, a detection apparatus, and a detection kit. This method for detecting an analyte in specimens comprises steps of: bringing a labeled reagent containing a ligand that specifically binds to the analyte into contact with a specimen; and supplying the mixture of the specimen and the labeled reagent to a solid-phase support onto which a capture reagent that specifically binds to the analyte has been immobilized, wherein the step of bringing the specimen into contact with the labeled reagent is carried out at a site that is not on the solid-phase support and that is detached from the solid-phase support.
    Type: Grant
    Filed: January 27, 2005
    Date of Patent: May 18, 2010
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Kazuyuki Takizawa, Ryo Shida
  • Patent number: 7704702
    Abstract: A test strip configured for the detection of an analyte in a fluid sample and methods for manufacturing and for using the same. The test strip comprises a first flow matrix and a second flow matrix sequentially arranged to form an interface therebetween. The first flow matrix comprises a detection composition movably bound thereto, wherein the detection composition when exposed to the analyte produces at least one detectable product and wherein the first and second solid matrices are selected so as to accumulate the at least one detectable product at the interface between the two matrices, when the fluid sample travels from the first flow matrix to the second flow matrix.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: April 27, 2010
    Assignee: Inverness Medical Switzerland GmbH
    Inventors: Tomer Keren, Oren Shraga Dwir
  • Patent number: 7659125
    Abstract: A latex reagent for analyzing adiponectin, comprising a suspension of latex particles carrying a substance which specifically binds to adiponectin, is disclosed. Further, a method for analyzing adiponectin, comprising (1) obtaining a biological liquid possibly containing adiponectin, and (2) bringing the biological liquid, while maintaining the state in which the biological liquid is obtained, into contact with a suspension of latex particles carrying a substance which specifically binds to adiponectin, and optically analyzing a degree of latex-particles-agglutination, is disclosed. According to the latex reagent and the method for analyzing adiponectin, a predilution or pretreatment of the biological liquid to be analyzed is not necessary. Further, the analysis can be performed rapidly and conveniently, and facilities therefor are not limited.
    Type: Grant
    Filed: March 24, 2004
    Date of Patent: February 9, 2010
    Assignee: Mitsubishi Kagaku Latron, Inc.
    Inventors: Tetsuya Tachikawa, Suguru Akamatsu, Tokio Sawai, Ayako Nishimura
  • Patent number: 7632686
    Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.
    Type: Grant
    Filed: October 2, 2003
    Date of Patent: December 15, 2009
    Assignee: Anderson Forschung Group
    Inventor: Norman L. Anderson
  • Publication number: 20090269859
    Abstract: It has been discovered that a column configuration which allows for suspension of the beads, such as a mobile bead, during washing results in significantly lower levels of background and hence more sensitive levels of quantitation. The present invention provides columns, kits, and methods for more sensitive detection of a toxin or other analyte.
    Type: Application
    Filed: January 25, 2007
    Publication date: October 29, 2009
    Applicant: Waters Investments Limited
    Inventor: Jianmin Liu
  • Patent number: 7569398
    Abstract: The present invention relates to a method of transporting an analyte present in a sample, to a method of concentrating an analyte present in a sample, and to a device for implementing these methods. In the method of transporting an analyte present in a sample of the present invention, a solution A in which the analyte is attached to magnetic particles is prepared from the sample; the solution A is introduced into a first container connected via a bottleneck to a second container; and the analyte attached to the magnetic particles is moved, by means of a magnetic system, from the first container to the second container via the bottleneck, the second container being filled with all or part of the solution A and/or with another solution.
    Type: Grant
    Filed: November 27, 2001
    Date of Patent: August 4, 2009
    Assignee: Commissariat a l'Energie Atomique
    Inventors: Pierre Puget, Patrick Pouteau, Frédéric Ginot, Patrice Caillat
  • Patent number: 7524641
    Abstract: Rare cells are separated from a sample fluid by a positive selection or negative selection antibody by centrifuging in a tube containing a harvesting float. The harvesting float has an axial passage and a density to settle in the sample fluid and expand the layer of the target component. The positive selection antibody is preferably coupled to a particulate carrier, such as a microbead, to attach to the target component. The negative selection antibody forms a complex or conjugate with a contaminating component. In the positive separation, the particulate carrier is recovered in the axial passage of the float.
    Type: Grant
    Filed: March 12, 2007
    Date of Patent: April 28, 2009
    Assignee: Becton, Dickinson and Company
    Inventors: Stewart Russell Jurgensen, Sheila Ann Lloyd
  • Patent number: 7517699
    Abstract: Methods and devices for the detection and/or quantification of an analyte in a sample are provided. These are positive detection methods and devices, in that the more analyte is present in the sample, the stronger the signal that is provided. Devices of the invention include a mobilization zone including a mobile or mobilizable detectable analyte analog, a sample application area, primary and secondary capture areas each including an immobilized binding partner having a binding affinity for the analyte being tested for a detectable analyte analog. The mobilization zone, sample application area, primary and secondary capture area are in fluid continuous contact with each other. In these devices, the first immobilized binding partner has an equal or lower apparent affinity for the analyte than it has for the detectable analyte analog.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: April 14, 2009
    Assignee: Quantrx Biomedical Corporation
    Inventors: Jeffrey S. Bauer, Timothy P. Hyatt, Huiying Wang, Robert L. Buck
  • Patent number: 7488606
    Abstract: A device for collecting oral liquids includes a lateral flow chromatography strip having a collection member. The collection member is separated from the remainder of the chromatography strip by a liquid impermeable removable barrier which prevents liquid in the collection member from entering the chromatography strip. Once adequate oral liquid has been collected (as indicated by a sample sufficiency indicator), the barrier is removed to allow oral liquids to flow through the strip. The liquids interact with binding partners in the strip to provide test results, such as an indication that an analyte of interest is present in the liquid. The strip may be contained in a housing with an access opening through which the removable barrier may be manipulated, and windows through which test results may be viewed. This device avoids reflux of reagents from the strip into the mouth of a test subject during use.
    Type: Grant
    Filed: August 19, 2005
    Date of Patent: February 10, 2009
    Assignee: QuantRX Biomedical Corporation
    Inventors: William H. Fleming, Timothy P. Hyatt, Dudley B. Thomas
  • Patent number: 7473563
    Abstract: Provided is sampling and testing device for the detection of specific molds, allergens, viruses, bacteria, fungi, and other protein containing substances. Embodiments of the device include a sampling member slideably engaged with a base that contains a lateral flow strip adapted to detect specific analytes of interest. The sampling member defines a solvent reservoir that stores an elution solvent in a fluid-tight manner before the device is used to sample and test environmental surfaces. During slideable withdrawal of the sampling member from the base, the elution solvent stored in the reservoir is automatically released to a wick assembly of the sampling member. The wick assembly includes a wick adapted to receive, distribute, and retain the elution solvent. After a user samples an environmental surface for an analyte of interest with the elution solvent wetted wick, the sampling member is returned to the base where the wick contacts the lateral flow strip contained in the base.
    Type: Grant
    Filed: April 3, 2006
    Date of Patent: January 6, 2009
    Assignee: The Clorox Company
    Inventors: Brendi M. Cumberland, Maha El-Sayed, Alan J. Fujii, Scott D. Manske, Elias A. Shaheen
  • Patent number: 7452731
    Abstract: A method for determining the risk of an individual of suffering from inflammation, opportunistic infection or disruption of immunoglobulin metabolism, comprising (a) determining the level of fragmentation or modification of Fc function of immunoglobulins in a sample taken from the individual and (b) determining thereby the risk of inflammation, impaired immune response or opportunistic infection. The invention also provides the use of a trypsin inhibitor in the manufacture of a medicament for use in the treatment or prevention of a disorder associated with elevated trypsin activity which is correlated with IgG fragmentation or modification.
    Type: Grant
    Filed: February 15, 2002
    Date of Patent: November 18, 2008
    Inventor: Ivan Mikhailovich Petyaev
  • Patent number: 7449329
    Abstract: A blood crossmatching apparatus, kit and methods for testing the compatibility of mammals for blood transfusion. Particulate layers in the apparatus allow nonagglutinated red blood cells to permeate through, while agglutinated red blood cells cannot. The apparatus also has a density solution layered above particulate layers. The density solution separates white blood cells from red blood cells in the whole blood when centrifuged, without lysing the red blood cells. Thus, the apparatus can be used to test whole blood.
    Type: Grant
    Filed: March 31, 2006
    Date of Patent: November 11, 2008
    Inventor: Anne S. Hale
  • Patent number: 7445941
    Abstract: The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.
    Type: Grant
    Filed: March 2, 2004
    Date of Patent: November 4, 2008
    Assignee: Biosite Incorporated
    Inventor: Kenneth F. Buechler
  • Patent number: 7374936
    Abstract: The present invention relates to the field of site directed therapy. More specifically the invention relates to site directed radio therapy, and provides a method for production of radioconjugates and an apparatus for radioimmunotherapy. The method, conjugates and apparatus can be practicalized without the need for radioactive shielding and/or airtight facilities. Without these restrictions the invention provides a simple and efficient means of therapy at the bed-side of the patient.
    Type: Grant
    Filed: December 17, 2001
    Date of Patent: May 20, 2008
    Assignee: Actinium Pharmaceuticals, Inc.
    Inventor: Maurits W Geerlings
  • Patent number: 7361480
    Abstract: The invention is directed to a method of detecting gluten-induced diseases such as e.g. celiac disease and dermatitis herpetiformis. Said diseases are indicated by the presence of autoantibodies against tissue transglutaminase tTG in the blood The test is carried out on a whole blood sample using tTG liberated from the red blood cells of the blood sample as an autoantigen. The liberated autoantigens react with the autoantibodies in the sample and form antigen-antibody complexes, which are detected. The presence of such complexes indicates the disease. The invention is also directed to the use of the autoantigen in a test and to a test-kit.
    Type: Grant
    Filed: April 24, 2002
    Date of Patent: April 22, 2008
    Inventors: Markku Mäki, Ilma Korponay-Szabo
  • Patent number: 7354776
    Abstract: A method and kit for processing and testing powdered samples are provided herein. The powdered sample is combined with a fluid to produce a slurry or liquid suspension, and the slurry is contacted with an immunochromatographic test strip containing detectable reagents that are immunoreactive with an analyte to be detected in the sample. The method is particularly useful for the analysis of agricultural products or crops, such as the identification of recombinant grains.
    Type: Grant
    Filed: December 14, 2000
    Date of Patent: April 8, 2008
    Assignee: Strategic Diagnostics Inc.
    Inventors: Tara Ann Kennedy, Alan Bruce McQuillin
  • Patent number: 7348185
    Abstract: A method for capturing a target substance in a sample, comprising the steps of preparing particles capable of capturing the target substance in a dispersed state in a fluid, aggregating the particles, and allowing the particles to capture the target substance in the sample by bringing the sample into contact with the particles aggregated.
    Type: Grant
    Filed: June 23, 2005
    Date of Patent: March 25, 2008
    Assignee: Canon Kabushiki Kaisha
    Inventor: Junta Yamamichi