Abstract: A method of three-dimensional (3D) printing includes applying a solution to a channel. The solution includes a plurality of anisotropic particles suspending in the solution. Acoustic waves are applied to the channel. The frequency of the acoustic waves is configured to organize the plurality of anisotropic particles into one or more columns of organized anisotropic particles. The channel is connected to a printhead and a waste outlet. The solution comprising the one or more columns of organized anisotropic particles is deposited on a substrate via the printhead outlet.

Abstract: Color quantification of chemical test pads and titration of analytes can be performed under different lighting conditions. In one embodiment, the lighting condition is estimated under which a digital image is captured and utilized to select a set of reference colors from which the quantified color is compared to determine the titration. In another embodiment, a plurality of comparisons are made with different lighting conditions with the result having the highest confidence level being selected to determine the titration.

Abstract: The present disclosure generally relates to systems and methods for partitioning species. In some embodiments, such systems can be used for determining one or more types of cancer. For example, certain aspects are generally directed to aqueous multiphase partitioning systems that can be used, for example, for distinguishing between different types of cancers, diagnosing subjects with cancer, or the like. In some cases, such systems can be identified using solvent properties such as the solvent dipolarity/polarizability difference (??*), the solvent hydrogen bond donor acidity difference (??), the solvent hydrogen bond acceptor basicity difference (??), and the electrostatic property difference (c) between two phases of the partitioning system. These may be within certain ranges in accordance with various embodiments.

Abstract: The present invention provides devices, systems, and methods, for performing biological and chemical assays.

Abstract: Methods of detecting platinum and copper in a test sample are provided. Kits for use in detecting platinum and copper in a test sample also are provided.

Abstract: Various embodiments of fluidic devices and methods of the present teaching can provide precision on-device loading of fluidic samples, and merging, mixing, and splitting of the fluidic samples, in illustrative embodiments as droplets, using pressures that can be provided by standard laboratory liquid handling equipment. Various embodiments of fluidic devices of the present teachings can provide on-device manipulation of accurate and precise fluidic volumes at the picoliter to nanoliter scale for each steps from fluidic sample loading to fluidic sample splitting. Various embodiments of fluidic elements of the present teachings, for example, but not limited by, various embodiments of fluidic traps of the present teachings, can have a constrained and measurable geometry, allowing for accurate and precise tuning of each fluidic sample volume throughout the on-device liquid handling process.

Abstract: An ostomy appliance having a signal generator adapted to give a user or a health care professional a warning in time to change the appliance before leakage occurs by predetermining leakage or potential leakage of stomal fluids.

Abstract: A point of use analyte detection and quantification system for agricultural applications is provided. Related methods are also provided.

Abstract: The disclosed technology relates to chemical entities for the detection of wounds, e.g., chronic wounds or infected wounds, including compositions, substrates, kits, dressing materials, and articles, and systems containing such compounds. The disclosed technology further relates to methods of using these compositions, kits and systems in diagnostic assays, and in the diagnosis and/or detection of chronic or infected wounds based on enzymatic action on specific moieties and/or reaction sites. The disclosed technology additionally relates to detection of pathogenic, e.g., bacterial and/or viral substances, such as enzymes and substrates, at the wound situs. Additional disclosure relates to methods of characterizing wounds based on expression of a plurality of markers and using such information to treat, manage, and follow-up patients suffering from chronic or infected wounds.

Abstract: A point of use analyte detection and quantification system for aquatic applications is provided. Related methods are also provided.

Abstract: The present invention is to provide a tissue slice selection method and an embedded block-preparing cassette which are capable of suppressing occurrence of errors in inspection results. The present invention makes it possible to suppress the occurrence of errors in inspection results by including: an embedded block-preparing process of preparing an embedded block by embedding both a biological tissue fragment and a standard substance in the same embedding agent; an embedded tissue slice preparing step of slicing the embedded block to prepare a sheet-like embedded tissue slice having a tissue slice and standard substance slices appearing on a surface thereof; and a tissue slice selecting step of selecting the tissue slice to be inspected, based on a light signal from the standard substance slice.

Abstract: A flow-cytometer has an excitation light source generating an excitation light that excites one or more bio-cells in a bio-sample carried by a flow path to luminesce. The flow-cytometer includes a spectrum dispersive element that disperses the luminescent light generated by the bio-sample into a photo-detector array. The flow-cytometer further includes a digital signal processor (DSP) that receives signals from the photo-detector array and generates a self-triggering signal based on the luminescent light generated by the bio-cells in bio-sample. The self-triggering signal triggers data capture in the DSP to improve synchronization with the data generated from signals received from the photo-detector array.

Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

Abstract: The present invention generally provides, in various embodiments, methods of analyzing samples having a low abundance of proteins, e.g., single cells, utilizing liquid chromatography and tandem mass spectroscopy (LC-MS/MS).

Abstract: Disclosed is a method for assisting prediction of onset of cerebral infarction, based on the number of red blood cells contained in a blood sample collected from a subject, comprising the steps of: calculating an exponent value for the prediction from a first measured value indicating red blood cell count measured by electrical resistance measurement method and a second measured value indicating red blood cell count measured by optical measurement method, comparing the exponent value with a reference range, and suggesting that the subject develops cerebral infarction when the exponent value is outside the reference range.

Abstract: The present invention pertains to an enzymatic measurement method using an antibody-enzyme complex or a nucleic acid probe measurement method using an enzyme-labeled nucleic acid probe, in both of which the quantification of a product of a reaction by an enzyme in the antibody-enzyme complex or the enzyme-labeled nucleic acid probe is performed by generating thio-NAD(P)H by an enzymatic cycling reaction using NAD(P)H, thio-NAD(P), and a dehydrogenase (DH), and measuring the amount of the generated thio-NAD(P)H or measuring a change in color caused by the generated thio-NAD(P)H. An enzymatic reaction system in which NAD(P) generated from NAD(P)H by the enzymatic cycling reaction is selectively reduced, is caused to coexist with the enzymatic cycling reaction. The present invention also pertains to a kit for enzyme immunoassay, and a kit for nucleic acid probe measurement.

Abstract: Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material.

Abstract: An ostomy appliance has an adhesive wafer with a backing layer and an adhesive layer attached to the backing layer. The adhesive wafer has sensing means capable of recording application of pressure to the adhesive wafer during application of the ostomy appliance to the skin.

Abstract: A system for the mass spectrometry analysis of an analyte includes a droplet ejection device, a spray head comprising a spray tip for ejecting the solvent as a spray, and a solvent delivery conduit for delivering solvent to the spray tip. The spray head includes a droplet inlet opening communicating with the surrounding atmosphere for receiving liquid droplets comprising the analyte. The droplet ejection device selectively ejects a liquid analyte droplet comprising the analyte through a surrounding atmosphere and the droplet inlet opening into a solvent flowing through the solvent delivery conduit. A method for the mass spectrometry analysis of an analyte is also disclosed.

Abstract: Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.

Abstract: Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer. The second layer may be substantially absorbent to infrared radiation. The second layer and the first layer may collectively define a set of wells. The first layer may define a base for each well of the set of wells. The second layer may define an opening for each well of the set of wells. At least one of the first layer and the second layer may define a sidewall for each well of the set of wells.

Abstract: The disclosure is directed to a device that includes an upper container configured to receive a fluid sample collected from a mammal into a first opening, the first opening opposite a second opening and a membrane covering at least a portion of the second opening, the membrane configured to allow transmission of a portion of the fluid sample through the membrane.

Abstract: The present invention falls within the field of medical apparatuses. Disclosed are a method and a device for identifying platelet aggregation, and a flow cytometer, which are used for accurately giving an alarm about a platelet aggregation during blood cell analysis.

Abstract: Devices, systems, and methods are provided for monitoring or analyzing excreta of a subject using a toilet. In one embodiment, the device includes a housing mounted adjacent a toilet bowl, a urinalysis module for deploying urinalysis strips into the toilet bowl to collect a urine sample from a subject's urine stream delivered into the toilet bowl and a urinalysis sensor to acquire data from the urinalysis strip related to the urine sample, and a stool analysis module including a camera oriented into the toilet bowl to provide images of contents of the toilet bowl. In addition, the device may include a uroflowmetry module and/or a biometric identification module. One or more sensors are provided for identifying when a subject uses the toilet, and a processor activates one or more of the modules when the sensors indicate a subject is using the toilet.

Abstract: A microfluidic device can include an upstream passage, a sample passage, a bifurcating passage, and a combining passage. The upstream passage can be configured to provide a focusing stream. The sample passage can be configured to provide a sample stream. The bifurcating passage can include a specified bifurcating flow resistance. The combining passage can be configured to create a combined stream from the focusing stream and the sample stream, where the focusing stream can direct the sample stream away from the upstream passage and toward the bifurcating passage. A first portion of the combined stream can be discharged through the bifurcating passage. The main discharge can be configured to discharge a second portion of the combined stream. The main discharge can include a main discharge resistance that is selectable to vary the main discharge resistance relative to the bifurcating flow resistance.

Abstract: A method for the microscopy scanning of a specimen. An immersion medium is used between a slide and a microscope objective, said immersion medium wetting a surface of the slide, and the microscope objective being relatively displaced over the surface of the slide for imaging. The surface is provided with a coating which repels the immersion medium.

Abstract: The present disclosure relates to a cell potential detection device, a method of manufacturing the cell potential detection device, and an information processing system that enable prevention of culture solution for a cell from leaking. The cell potential detection device includes: a cell potential detection chip including an electrode unit that detects potential of a cell; a substrate on which the cell potential detection chip is implemented; a first member sealing a connection electrically connecting the cell potential detection chip and the substrate; and a second member layered on the first member, the second member forming a liquid-storage portion that stores culture solution for the cell, together with the first member. The present technology can be applied to, for example, a semiconductor module in which packaged is a chip that detects the potential at an action-potential source point due to a chemical change of a cell.

Abstract: A method is provided for testing for presence of a particulate selected from the group consisting of: a microorganism, a fungus, a bacteria, a spore, a virus, a mite, a biological cell, a biological antigen, a protein, a protein antigen, and a carbohydrate antigen. The method includes (a) collecting, in a tube (22), fluid that potentially contains the particulate, (b) using a plunger (24) to push the fluid through a filter (26) disposed at a distal portion of the tube or at a distal end of the plunger, and subsequently, (c) while the filter is inside the tube, ascertaining if any of the particulate was trapped by the filter by applying a particulate-presence-testing-facilitation solution to the filter. Other embodiments are also described.

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

Abstract: A method and device for detecting pyrethroid pesticide residues in crops. Reaction membrane is arranged on a bottom plate and provided with a check-up line and a quality control line; a first mounting block and a second mounting block are arranged on the bottom plate; a first slide is arranged in the first mounting block, a second slide is arranged in the second mounting block; a sample pad and a bonding pad are arranged in the first slide; a water absorption pad is arranged in the second slide; a liquid inlet provided with a pipe is formed in the first mounting block, a pressing hole provided with a press block is formed in the second mounting block; protrusions are respectively formed on the pipe and the press block; sliding grooves are formed in the liquid inlet and the pressing hole; and first springs are arranged between the protrusions and the sliding grooves.

Abstract: A nano-fluidic device includes: a first substrate that has a nanoscale groove on one surface; and a second substrate that is integrally provided with the first substrate by bonding one surface of the second substrate to the one surface of the first substrate and forms a nanochannel with the groove of the first substrate, in which either the first substrate or the second substrate includes at least a thin portion in a part of a position overlapping the nanochannel in plan view, and the thin portion is deformed by pressing to open and close the nanochannel.

Abstract: The systems and methods of the present disclosure are used for guiding a medical instrument towards a target, the method positioning a medical instrument at a first location within a patient anatomy, wherein the medical instrument comprises at least one sensor, determining a first biomarker measurement using the at least one sensor, determining a second biomarker measurement using the at least one sensor, comparing the first biomarker measurement with the second biomarker measurement to determine a proximity to the target to provide a first comparison, and providing guidance for moving the medical instrument based on results of the first comparison.

Abstract: The present invention provides a pipette tip, which can be used in in-vitro diagnostics, in particular in the diagnostic testing of body fluids, such as in coagulation testing. The Pipette tip contains two constituents in a spatially separated manner. The present invention furthermore provides a method of performing such diagnostics, e.g. coagulation analysis, and to the use of the pipette tip in such diagnostic testing.

Abstract: A low-noise current sensor enables large dynamic range current measurements of alternating and direct current flows. The current sensor includes a first substrate, a first conductor, a magnetic flux conductor comprising a first portion orthogonal to the first conductor and a second portion, the second portion penetrating the first substrate, an inductive sensor comprising a first plurality of loops around the second portion of the magnetic flux conductor on the first substrate, wherein the first plurality of loops is orthogonal to the second portion of the magnetic flux conductor, and a Faraday cage that encloses the first plurality of loops and separates the first plurality of loops from the first conductor.

Abstract: The invention relates to an in vitro method for determining the adsorbing capacity of an adsorbent having limited solubility, such as a bile acid sequestrant, under conditions simulating the mammalian gastrointestinal tract. The method is particularly useful for studying the release profiles of controlled release formulations comprising adsorbents having limited solubility.

Abstract: The use of volatilization reagents is disclosed for improved detection of inorganic oxidizers such as, but not limited to, chlorates and perchlorates. Detection methods are disclosed whereby a reagent can transfer a proton to the anion (i.e., chlorate, perchlorate, etc.) of an inorganic salt analyte, forming an acid (i.e., chloric acid, perchloric acid) that is easier to detect by a mechanism whereby the acidified reagent is more easily vaporized, and hence, more easily detected. Concurrently, the anion of the acid forms a new salt with the cation released from the salt that was acidified. The reagents can also include acidic salts or cation-donators, more generally. In some embodiments, hydrated reagents or co-reagents that can release water can be employed.

Abstract: A method of automated measurement of motility and morphology parameters of the same single motile sperm. Automated motility and morphology measurements of the same single sperm are performed under different microscope magnifications. The same single motile sperm is automatically positioned and kept inside microscope field of view and in focus after magnification switch. A method of automated non-invasive measurement of sperm morphology parameters under high magnification of imaging. Sperm morphology parameters including subcellular structures are automatically measured without invasive sample staining. A method of automatically selecting sperms with normal motility and morphology and DNA integrity for infertility treatment.

Abstract: A device for analysing a sample comprising a nucleic acid to be captured and detected using a test strip are described. The device comprises a resilient biasing member disposed in an analysis chamber containing the test strip. The resilient biasing member exerts a force against the test strip sufficient to urge it into the sample chamber when it is in communication with the analysis chamber. This ensures that the test strip is reliably introduced into the sample chamber when it is in communication with the analysis chamber. In one embodiment, the sample chamber comprises guide members for guiding the test strip into the sample chamber. A free end of each guide member is shaped to prevent significant rotation of the test strip, so that the test strip is in correct alignment in the sample chamber for automatic reading of the test result, for example by a camera or optical reader.

Abstract: The present invention provides a bio-electrode composition including a silsesquioxane bonded to an N-carbonyl sulfonamide salt, wherein the N-carbonyl sulfonamide salt is shown by the following general formula (1): wherein R1 represents a linear, branched, or cyclic alkylene group having 1 to 20 carbon atoms that may have an aromatic group, an ether group, or an ester group, or an arylene group having 6 to 10 carbon atoms; Rf represents a linear, branched, or cyclic alkyl group having 1 to 4 carbon atoms containing at least one fluorine atom; M+ is an ion selected from a lithium ion, a sodium ion, a potassium ion, and a silver ion. This can form a living body contact layer for a bio-electrode that is excellent in electric conductivity and biocompatibility, light-weight, manufacturable at low cost, and free from large lowering of the electric conductivity even though it is wetted with water or dried.

Abstract: Methods are described for measuring the amount of one or more of vitamin A, ?-tocopherol, and the combination of ?-tocopherol and ?-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, ?-tocopherol, and the combination of ?-tocopherol and ?-tocopherol in a sample.

Abstract: Disclosed herein include systems, devices, and methods for determining a gating strategy. An acquisition system can cause a particle analyzer to collect parameter measurements of some particles of a sample. An analysis system can receive the parameter measurements and determine a gating strategy from the parameter measurements. The acquisition can collect measurements of some or all of the remaining particles of the sample using the gating strategy determined by the analysis system.

Abstract: A liquid droplet and solid particle sensing device is provided that can measure the average droplet size in a spray. The present device uses a swirling flow to draw a particulate or a spry into the device for sizing and counting. The swirling flow is configured to keep all the particles away from the walls of the device and to concentrate them at the center of a flow channel to pass through the center of a light beam for high sensitivity and repeatability of the measurement.

Abstract: A system for the differentiation of plastic and non-plastic particles in suspension in a liquid, and the method of use thereof. The system having a channel for constraining and presenting the liquid to a detector, the detector having at least one transmit electrode for emitting electrical current to at least one receive electrode. Circuitry provides the current input and received electrical signals measurement capacities. Particles passing the receive electrodes alter the received electrical current according to the particle's dielectric properties, the circuitry records the received signals and discerns a particle's nature, most often plastic from non-plastic, from the differential signal of these received signals as a function of frequency.

Abstract: A blood cell analysis method and a blood cell analyzer are provided. In the method and analyzer, characteristic information of white blood cell fragments is obtained based on side scattered light information and fluorescence information, characteristic information of platelets is obtained based on forward scattered light information and fluorescence information and then a count value for the platelets is acquired based on the characteristic information of the platelets and the characteristic information of the white blood cell fragments. The present invention can avoid the influence of the white blood cell fragments on the platelet counting, thereby ensuring the accuracy of the platelet counting without increasing costs.

Abstract: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

Abstract: A method of detecting the presence of a prostate cancer in a human subject comprising the steps of (a) obtaining a histologically normal prostate tissue sample from the patient and (b) quantifying the epithelial thickness or gland lumen roundness of the tissue, wherein an increase in epithelial thickness or a decrease in gland lumen roundness indicates the presence of prostate cancer or a prostate cancer field defect.

Abstract: The invention relates to a method for detecting an analyte present in a liquid specimen, including: injecting the liquid specimen into a detection chamber, the detection chamber having a non-zero volume enclosing polymeric beads covered with a reagent suitable for the analyte to be detected; capturing at least one image of at least one region of the detection chamber using a sensor; processing the image acquired by the sensor, which includes determining a texture level of the acquired image; and determining a concentration of the analyte depending on the texture level determined for the image.

Abstract: Disclosed are methods and systems for measuring serotonin in a sample using liquid chromatography and mass spectrometry.

Abstract: Embodiments herein include a method for detecting a brain condition in a subject. The method can include obtaining a breath sample from the subject and contacting it with a chemical sensor element, where the chemical sensor element includes a plurality of discrete graphene varactors. The method can include sensing and storing capacitance of the discrete graphene varactors to obtain a sample data set and classifying the sample data set into one or more preestablished brain condition classifications. Other embodiments are also included herein.

Abstract: Provided are methods for determining the amount of tamoxifen and its metabolites in a sample by mass spectrometry. In some aspects, the methods provided herein determine the amount of norendoxifen. In some aspects, the methods provided herein determine the amount of norendoxifen and tamoxifen. In some aspects, the methods provided herein determine the amount of norendoxifen and other tamoxifen metabolites. In some aspects, the methods provided herein determine the amount of tamoxifen, norendoxifen, and other tamoxifen metabolites.