Biological Cellular Material Tested Patents (Class 436/63)
  • Patent number: 11016062
    Abstract: A novel SH-SAW biosensor capable of non-invasive and touch-free detection of cancer cell viability and growth or proliferation in two-dimensional (2D) and three-dimensional (3D) cell cultures as well as stem cell regeneration as it pertains to cancer cell biology and anti-cancer drug development is presented. The biosensor includes two pairs of resonators including interdigital transducers reflecting fingers to quantify mass loading by the cells in suspension as well as within a tumoroid culture platform. The biosensor can be part of a perfused 3PNS-tumoroid system that is amenable to real-time non-invasive monitoring of the cell proliferation, viability, and multiplexed detection of key physiologic and clinical biomarkers.
    Type: Grant
    Filed: December 20, 2019
    Date of Patent: May 25, 2021
    Assignees: University of South Florida, TransGenex Nanobiotech, Inc.
    Inventors: Shyam S. Mohapatra, Subhra Mohapatra, Rasim Oytun Guldiken, Rajesh R. Nair, Tao Wang
  • Patent number: 10928401
    Abstract: The present disclosure provided methods and systems for diagnosing diseases and monitoring their progression and therapeutic responses by detecting a presence or absence, or an increase or decrease, of one or more substances in a sample.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: February 23, 2021
    Assignee: NEWOMICS INC.
    Inventors: Daojing Wang, Pan Mao
  • Patent number: 10859586
    Abstract: Disclosed are methods and systems for measuring serotonin in a sample using liquid chromatography and mass spectrometry.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: December 8, 2020
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Matthew Lee Francis Crawford, Yvonne Zamorano Wright, Russell Philip Grant
  • Patent number: 10830746
    Abstract: Provided are methods for determining the amount of tamoxifen and its metabolites in a sample by mass spectrometry. In some aspects, the methods provided herein determine the amount of norendoxifen. In some aspects, the methods provided herein determine the amount of norendoxifen and tamoxifen. In some aspects, the methods provided herein determine the amount of norendoxifen and other tamoxifen metabolites. In some aspects, the methods provided herein determine the amount of tamoxifen, norendoxifen, and other tamoxifen metabolites.
    Type: Grant
    Filed: December 4, 2017
    Date of Patent: November 10, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Nigel Clarke
  • Patent number: 10821441
    Abstract: A microfluidic device includes a substrate and a cover. The substrate has an inlet port, a first microchannel, one or more parking loops, a second microchannel and an outlet port for each microchannel network. The first microchannel is connected to the inlet port, the second microchannel is connected to the outlet port, the parking loops are connected between the first and second microchannels. Each parking loop includes a parking loop inlet, a parking loop output, a fluidic trap connected between the parking loop inlet and the parking loop outlet, and a bypass microchannel connected to the parking loop inlet and the parking loop outlet. The cover is attached to a top of the substrate and has an inlet opening and an outlet opening through the cover for each microchannel network. The inlet and outlet openings of the cover are disposed above the inlet and outlet ports in the substrate.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: November 3, 2020
    Assignee: Texas Tech Univeristy System
    Inventors: Siva A. Vanapalli, Swastika S. Bithi
  • Patent number: 10683496
    Abstract: A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.
    Type: Grant
    Filed: May 22, 2018
    Date of Patent: June 16, 2020
    Assignee: General Electric Company
    Inventors: Lin Chen, Bing Zhang, Klaus Hochleitner, Rong Hou, Yanju Wang
  • Patent number: 10585080
    Abstract: A pressure sensor and its use for visually determining whether a preselected pressure threshold has been achieved, for example during high pressure processing treatment of a foodstuff. The pressure sensor includes a contained color-changing system having a dye, a developer, and a solvent; upon achievement of the preselected pressure threshold, the dye and the developer interact, resulting in a visible color change. Further, the visible color change can be retained upon a decrease in pressure and upon an increase in temperature, thereby effectively recording the achievement of the preselected pressure threshold during the high pressure processing treatment.
    Type: Grant
    Filed: July 8, 2019
    Date of Patent: March 10, 2020
    Assignee: Chromatic Technologies, Inc.
    Inventors: Ruizheng Wang, Timothy J. Owen, Lyle D. Small
  • Patent number: 10569272
    Abstract: A sorting device (10) is disclosed comprising a channel (40) adapted to allow passage of a plurality of samples in a fluid to a first side of a sorting junction (50) and a plurality of Braille valves (105) connected by a plurality of connectors to the sorting junction (50). An analysis device (80), such as a camera, is used for analysing ones of the plurality of the samples and adapted to control ones of the plurality of Braille valves depending on properties of the analysed ones of the plurality of the samples.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: February 25, 2020
    Inventors: Christoph Merten, Qingzong Tseng, Ramesh Utharala, Lukas Frese
  • Patent number: 10534008
    Abstract: The invention relates to the detection of DHA and EPA. In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry and kits for carrying out such methods.
    Type: Grant
    Filed: June 28, 2017
    Date of Patent: January 14, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Scott M. Goldman, Julie A. Neidich
  • Patent number: 10458980
    Abstract: A method of preparing a cantilever sensor for measuring biochemical interactions and their associated stress wherein a cantilever having two sides is coated on one side with at least a gold layer and both sides of the cantilever are functionalized with a self-assembled monolayer (SAM) of a probe molecule by incubating the cantilever in a solution having a concentration of the probe molecule of between 1 to 1000 ?M. The unpassivated cantilever sensor comprising a layer coated on one side with a coating comprising gold and being unpassivated on the opposite side, wherein both surfaces comprises a self-assembled monolayer of a probe molecule in which the surface area occupied per probe molecule varies in the range 0.4-1.5 nm2, enabling the stress at the gold top surface that is not cancelled out by a counter stress from the bottom surface so that accurate quantitation of a target molecule is achieved.
    Type: Grant
    Filed: June 23, 2016
    Date of Patent: October 29, 2019
    Assignee: 3P SENSE LIMITED
    Inventors: Joseph Wafula Ndieyira, Samadhan Patil, Giuseppe Mazza, Massimo Pinzani
  • Patent number: 10338369
    Abstract: A method for setting an evaluation parameter for a fluorescence microscope includes exciting dye particles in a sample to fluoresce and detecting fluorescent light from the particles. A graphical representation of a distribution of the fluorescent light is determined and a signal is generated for use in displaying the graphical representation on a display unit. Each subregion of the graphical representation is associated with a comparison value that is representative of a light quantity in the subregion. A predefined threshold is used as an evaluation parameter and compared to the comparison values. The subregions having a comparison value that is greater than the threshold value are marked on the display unit with predefined markings. The threshold value is changed and the comparison values are compared to the changed threshold value. The marked regions are defined as events and a complete image of the sample is obtained based on the events.
    Type: Grant
    Filed: January 3, 2011
    Date of Patent: July 2, 2019
    Inventor: Jonas Foelling
  • Patent number: 10279345
    Abstract: Fluidic devices and methods involving incubation and/or mixing of assay components are provided. In some embodiments, a biological and/or chemical assay may be performed in a fluidic device. The fluidic device may be designed to allow for controlled incubation and/or mixing of two or more assay components. In some such embodiments, the fluidic device may include an incubation channel having a relatively large cross-sectional dimension in fluid communication with a detection channel. The incubation channel may allow for adequate mixing and/or incubation of two or more assay components prior to analysis of the assay. In certain embodiments, the detection channel may be used to provide feedback on the extent of incubation and/or mixing. Based on the feedback, one or more component of the fluidic system may be regulated to allow the requisite degree of mixing and/or incubation to be achieved.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: May 7, 2019
    Assignee: OPKO Diagnostics, LLC
    Inventors: Matthew Dirckx, Jason Taylor, Vincent Linder
  • Patent number: 10234397
    Abstract: A reagent for detection of urine monophenolic metabolites and a method for preparing the reagent are disclosed, in which the monophenolic metabolites, for example, tyrosine, p-hydroxyphenyl alanine, tryptophan and 5-hydroxyindoleacetic acid can serve as tumor markers. The reagent for detection of urine monophenolic metabolites is an aqueous solution containing nitric acid, sulfuric acid, mercuric sulfate, mercurous nitrate, nickel nitrate, phosphomolybdic acid and cobalt sulfate. The preparation method includes preparation of solutions A, B, C, D and E, and mixing.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: March 19, 2019
    Inventors: Dong Liu, Lei Li, Mingbin Zheng
  • Patent number: 10190963
    Abstract: Methods and systems are disclosed for generating an entrainment factor in a flow cytometry sample. The methods comprise flowing a sample with a series of particles through the flow cytometer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the flow cytometer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: January 29, 2019
    Assignee: Becton, Dickinson and Company
    Inventors: Joseph Trotter, Sujata Iyer
  • Patent number: 10126232
    Abstract: A sample test method, microfluidic device, and test device efficiently and accurately compensates for interference of an interfering substance present in a sample using optical measurement without addition of a separate reagent for detecting the interfering substance. The sample test method includes: measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: November 13, 2018
    Inventors: Sung Ha Park, Sang Bum Park, Beom Seok Lee, Kui Hyun Kim, Joo Hee Park, Kyung Mi Song, Euy Hyun Cho, Ha Na Kim
  • Patent number: 10113774
    Abstract: Materials, components, and methods consistent with the present invention are directed to the fabrication and use of micro-scale channels with a fluid, where the temperature and flow of the fluid is controlled through the geometry of the micro-scale channel and the configuration of at least a portion of the wall of the micro-scale channel and the constituent particles that make up the fluid. Moreover, the wall of the micro-scale channel and the constituent particles are configured such that collisions between the constituent particles and the wall are substantially specular.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: October 30, 2018
    Assignee: Forced Physics, LLC
    Inventor: Scott Davis
  • Patent number: 10035848
    Abstract: An anti-C3d antibody or antibody fragment; method for use thereof to kill cancer cells; and related methods and compositions.
    Type: Grant
    Filed: January 8, 2015
    Date of Patent: July 31, 2018
    Assignees: The United States of America, as represented by the Secretary, Department of Health and Human Services, University of Virginia Patent Foundation
    Inventors: Adrian U. Wiestner, Martin W. Skarzynski, Margaret A. Lindorfer, Ronald P. Taylor, Christoph Rader, Berengere Vire
  • Patent number: 10006840
    Abstract: The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: June 26, 2018
    Assignee: Miltenyi Biotec GmbH
    Inventors: Burgund Kauling, Volker Huppert
  • Patent number: 9975913
    Abstract: The present invention relates to a method for in-gel visual detection and quantitative detection of proteins in activity based protein profiling (ABPP) using horseradish peroxidase mimic FeIII-TAML complex of ligand as a catalytic probe. The invention further relates to kit comprising compounds of formula (I) and method for the detection of bioanalytes using kit comprising compounds of formula (I).
    Type: Grant
    Filed: July 5, 2016
    Date of Patent: May 22, 2018
    Assignee: Council of Scientific & Industrial Research
    Inventors: Sayam Sen Gupta, Sushma Kumari, Chakadola Panda
  • Patent number: 9966458
    Abstract: The present invention relates to a method and a device for providing a current of spin-polarized electrons. More particularly, the present invention is suited for use in spin electronics or detection of spin-polarized electrons.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: May 8, 2018
    Assignees: Westfälische Wilhelms Universität Münster, Yeda Research and Development Co. Ltd. at the Weizmann Institute of Science
    Inventors: Benjamin Göhler, Volker Hamelbeck, G. Friedrich Hanne, Helmut Zacharias, Ron Naaman, Tal Zvi Markus
  • Patent number: 9897530
    Abstract: Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: February 20, 2018
    Assignees: Sony Corporation, Sony Corporation of America
    Inventors: Gary Durack, Stephen D. Fleischer, Jeremy Hatcher, David Roberts, Michael Zordan
  • Patent number: 9874537
    Abstract: The concentration measurement method includes: introducing a predetermined amount of the biological sample into the capillary; measuring a temperature of the biological sample by applying a first voltage to the electrode unit when the temperature of the biological sample is measured, the first voltage allowing the temperature measurement to be less affected by increase and reduction in an amount of the analyte contained in the biological sample; measuring the concentration of the analyte contained in the biological sample by applying a second voltage to the electrode unit; measuring an environmental temperature in a surrounding of the biological sample; and correcting the concentration of the measured analyte based on the measured temperature of the biological sample and the measured environmental temperature.
    Type: Grant
    Filed: March 7, 2017
    Date of Patent: January 23, 2018
    Inventor: Masaki Fujiwara
  • Patent number: 9857361
    Abstract: The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. A particle imaging system or analyzer can include a flowcell through which a urine sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis. A contrast pattern for autofocusing is provided on the flowcell. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Cell or particle images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: January 2, 2018
    Assignee: IRIS International, Inc.
    Inventors: Bart J. Wanders, Eric Chapoulaud, Brett Jordan
  • Patent number: 9835606
    Abstract: Provided are methods for determining the amount of tamoxifen and its metabolites in a sample by mass spectrometry. In some aspects, the methods provided herein determine the amount of norendoxifen. In some aspects, the methods provided herein determine the amount of norendoxifen and tamoxifen. In some aspects, the methods provided herein determine the amount of norendoxifen and other tamoxifen metabolites. In some aspects, the methods provided herein determine the amount of tamoxifen, norendoxifen, and other tamoxifen metabolites.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: December 5, 2017
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Nigel Clarke
  • Patent number: 9830553
    Abstract: A code book is generated for mapping source to target code words which allows encoding source data at reduced probability of incorrect decoding, e.g. for DNA storage. The target code words are grouped (102) into subsets and comprise identifying and remaining portions. The identifying portions of target code words corresponding to a same subset are identical. A first code symbol set of source code words is selected (103) for addressing the subsets. For the subsets, neighboring subsets are determined (104). The identifying portions of the target code words of neighboring subsets differ from those of the corresponding subset by up to a predetermined amount of symbols. Source code words are assigned (105) where the corresponding first code symbols address the same subset to said subset such that an amount of target code words of said subset having their remaining portions identical to their neighboring subsets corresponds to an optimization criterion.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: November 28, 2017
    Inventors: Xiaoming Chen, Meinolf Blawat, Klaus Gaedke, Ingo Huetter
  • Patent number: 9829436
    Abstract: The present invention relates to enhancement of detectable emissions from carbon nanodots or variants thereof by using the techniques of MEF to further enhance carbon nanodot brightness, photostability, and thus, potentially detectability in biological imaging applications by using plasmon supporting materials, such as silver island films and positioning of the carbon nanodots an optimal distance from the plasmon supporting materials.
    Type: Grant
    Filed: March 19, 2012
    Date of Patent: November 28, 2017
    Inventor: Chris D. Geddes
  • Patent number: 9817003
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort including centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using 9-oxo-ODE as biomarker.
    Type: Grant
    Filed: September 19, 2014
    Date of Patent: November 14, 2017
    Assignee: NESTEC S.A.
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Patent number: 9802192
    Abstract: A sorting chamber (1) for sorting first particles (25) from second particles (26) comprising: an internal chamber (7), which is delimited by a base wall (2), a top wall (4), and a spacer (6), set between the base wall (2) and the top wall (4); an internal chamber (7), which is at least partially-delimited by the base wall (2) and top wall (4); two passages (8), which set in communication the internal chamber (7) with the external environment; and a plurality of cavities (9), which are designed to house the first particles (25), are made in the base wall (2) and have openings (10) towards the internal chamber (7) with widths of from 4 to 6 ?m.
    Type: Grant
    Filed: October 1, 2009
    Date of Patent: October 31, 2017
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventors: Giuseppe Giorgini, Gerardo Perozziello, Alex Calanca, Gianni Medoro, Nicolò Manaresi
  • Patent number: 9797909
    Abstract: A sensor for detecting and/or quantifying the amount of analyte in a sample, the sensor including: a sensing region; and a barrier layer including a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent adsorbed thereto; wherein the sensor is adapted so that the sample enters the sensing region of the sensor through the barrier layer.
    Type: Grant
    Filed: September 26, 2016
    Date of Patent: October 24, 2017
    Assignee: Lightship Medical Limited
    Inventors: William Paterson, Nick Barwell, Bruce Culbert, Barry Colin Crane
  • Patent number: 9737260
    Abstract: The present invention is based on the finding that enrichment of D3-creatinine in a urine sample following oral administration of a single defined dose of D3-creatine can be used to calculate total-body creatine pool size and total body skeletal muscle mass in a subject. The invention further encompasses methods for detecting creatinine and D3-creatinine in a single sample. The methods of the invention find use, inter alia, in diagnosing disorders related to skeletal muscle mass, and in screening potential therapeutic agents to determine their effects on muscle mass.
    Type: Grant
    Filed: April 13, 2016
    Date of Patent: August 22, 2017
    Inventors: Marc K. Hellerstein, William Evans
  • Patent number: 9739658
    Abstract: The present invention relates to a cell peeling recognizing device and a cell peeling recognizing method. When a moving part (robot 6) moves culture vessel 5, imaging device 14 images an interior of culture vessel 5 a plurality of times as the cells move inside the culture vessel due to the moment of inertia, and recognizing part 10a recognizes the peeled state of cells C by comparing various sets of the captured imaging data. The peeled state of cells in the culture vessel can be determined with high precision.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: August 22, 2017
    Inventor: Daisuke Watanabe
  • Patent number: 9694177
    Abstract: A device for electrically controlled of ions, comprises a first electrolyte; a first electrode, which is arranged in direct or indirect contact with the first electrolyte, an encapsulation; and an ion conductor, which is arranged to receive and/or deliver ions from/to the first electrolyte. The encapsulation is arranged to effectively enclose the first electrolyte, and the ion conductor is arranged to transport ions from/to an outside of the encapsulation.
    Type: Grant
    Filed: March 20, 2008
    Date of Patent: July 4, 2017
    Assignee: OBOE IPR AB
    Inventors: Magnus Berggren, Joakim Isaksson, Edwin Jager, Peter Kjall, David Nilsson, Agneta Richter-Dahlfors, Daniel T. Simon, Klas Tybrandt, Barbara Canlon
  • Patent number: 9664698
    Abstract: The present invention inter alia provides a method, and uses thereof, of predicting statin-induced muscle toxicity or its complications, such as myalgia, myopathy and rhabdomyolysis, by detecting the lipid concentrations or lipid ratios of a biological sample and comparing it to a control. This method has identified lipid markers that are more specific and sensitive in detecting these statin-induced muscle toxicity than the currently utilized clinical markers. Also provided is an antibody towards said lipids, and the use thereof for predicting, diagnosing, statin-induced muscle toxicity. The invention additionally relates to kits comprising lipids and/or an antibody thereto, for use in the prediction and/or diagnosis of statin-induced muscle toxicity.
    Type: Grant
    Filed: April 8, 2011
    Date of Patent: May 30, 2017
    Inventors: Reijo Laaksonen, Kim Ekroos, Reini Hurme, Minna Janis, Riikka Katainen, Kirill Tarasov
  • Patent number: 9528981
    Abstract: The invention proposes a novel therapeutic and diagnostic methodology based on the use of effector cells (for example CTL and NK cells) selected for being able to induce lysis in target cells. In particular, the invention teaches how to select strains of effector cells of the immune system according to their lytic properties. It also teaches how diagnostic procedures for checking the activity of therapeutic vaccines can be improved with respect to what shown. Finally, it shows how cell therapies used at present can be improved by using this approach.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: December 27, 2016
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventors: Roberto Gambari, Roberto Guerrieri
  • Patent number: 9502227
    Abstract: A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: November 22, 2016
    Assignee: HUMANIX CO., LTD.
    Inventors: Tsutomu Masujima, Naohiro Tsuyama, Hajime Mizuno
  • Patent number: 9488621
    Abstract: The present invention is a method and apparatus for acoustically manipulating one or more particles.
    Type: Grant
    Filed: August 10, 2012
    Date of Patent: November 8, 2016
    Assignee: Los Alamos National Security, LLC
    Inventors: Gregory Kaduchak, Michael D Ward
  • Patent number: 9422588
    Abstract: An apparatus for measuring bacteria includes a sampling device for processing fluorescently stained bacteria, a detector for detecting size information from the bacteria in the sample, and a detector for detecting fluorescence information expressing intensity of fluorescent light emitted by the bacteria. The apparatus further includes a processor and memory that includes programs for creating a scattergram representing a distribution of the bacteria based on the size information and the fluorescence information detected, analyzing the distribution of the bacteria in the scattergram, and determining whether the bacteria in the sample is bacillus or coccus based on a result of the analysis.
    Type: Grant
    Filed: January 9, 2014
    Date of Patent: August 23, 2016
    Inventor: Yasuyuki Kawashima
  • Patent number: 9418825
    Abstract: A mass spectrometry is equipped with a liquid specimen supply part which supplies a liquid specimen sandwiched between bubbles, an ion source part ionizes the specimen, and a mass spectrometry part which detects ions separated in accordance with mass. In particular the ion source part is configured so as to include a liquid supply tube for transporting a specimen from the liquid specimen supply part, a degassing/liquid retention part in which bubbles are removed, a spraying part which ionizes the specimen, and a high-voltage power supply part which applies a high voltage to the spraying part. The device is further characterized in that after removing the bubbles, a Taylor cone is formed from the resultant pre-solution, and the specimen is ionized thereafter. Thus, the ionization of an intended specimen is stabilized, and the measurement reproducibility is improved.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: August 16, 2016
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Hiroyuki Yasuda, Shinya Ito, Akira Fujii, Yasushi Terui
  • Patent number: 9404918
    Abstract: It is provided a method for detection and/or quantification of at least one molecule presents in blood by a MALDI-MS analysis of a dried fluid spot without the presence of any digestion step or liquid extraction step, which permits to further analyze the physical distribution of at least one molecule within a dried fluid spot.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: August 2, 2016
    Assignee: Zentech
    Inventors: Maria D'Aloia, Nicolas Smargiasso, Delphine Debois, Edwin De Pauw
  • Patent number: 9400236
    Abstract: Devices and methods for magnetophoretic analyte selection and concentration are described. Magnetically marked analytes (e.g., cells) may be separated out of a sample dynamically in flux, such that the magnetically marked analytes are present in a highly concentrated manner in a reduced sample volume. The analyte selection may be followed by an analysis.
    Type: Grant
    Filed: May 11, 2012
    Date of Patent: July 26, 2016
    Assignee: Siemens Aktiengesellschaft
    Inventor: Oliver Hayden
  • Patent number: 9389162
    Abstract: A device and a method for detecting an analyte in a liquid sample. In an aspect, a mixing portion is configured to receive and mix a first liquid solution of unknown composition with a second liquid solution that includes a suspended particle resulting in a mixed liquid solution, wherein a surface of the suspended particle is functionalized to target an analyte. Furthermore, in an aspect, a surface of a solid substrate is configured to receive and evaporate a drop of the mixed liquid solution, wherein evaporation of the drop in connection with a capillary flow of the mixed liquid solution disperses the suspended particle in a ring pattern. The presence or absence of an analyte in the first liquid solution can be determined by visual inspection of the ring pattern.
    Type: Grant
    Filed: April 10, 2014
    Date of Patent: July 12, 2016
    Inventors: Ting-Hsuan Chen, Wei Liu, Yuanhang Li
  • Patent number: 9377390
    Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: June 28, 2016
    Assignee: Inguran, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
  • Patent number: 9360446
    Abstract: Methods and systems for measuring and using the oxidation-reduction characteristics of a biological sample are provided. The system generally includes a test strip and a readout device. A fluid sample is placed in the test strip, and the test strip is in turn operatively connected to the readout device. The readout device provides a controlled current that is sent across the fluid in the sample chamber. In addition, the readout device identifies an inflection point or transition time at which the voltage between contacts of the test strip is changing at the highest rate. The oxidation-reduction capacity of the sample is taken as the integral of the current profile from the time at which current begins to be supplied to the sample to the identified transition time.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: June 7, 2016
    Assignee: Aytu BioScience, Inc.
    Inventors: Raphael Bar-Or, David Bar-Or, Leonard T. Rael
  • Patent number: 9347885
    Abstract: The method of detecting thalassemia by optical analysis of blood components is a spectral detection method that is based on the fluorescence spectra of a set of biomolecules, including tyrosine, tryptophan, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide, which are all found in blood plasma, and porphyrin, which is found in red blood cells (RBCs). Measured ratios of intensity maxima between tryptophan and nicotinamide adenine dinucleotide, flavin adenine dinucleotide and nicotinamide adenine dinucleotide, tyrosine and tryptophan, and the normal form of porphyrin and the basic form of porphyrin may each be used, alone or in combination, to diagnose a patient as suffering from thalassemia.
    Type: Grant
    Filed: May 7, 2013
    Date of Patent: May 24, 2016
    Inventors: Mohamad Saleh Al Salhi, Vadivel Masilamani, Farjah Hassan Algahtani
  • Patent number: 9315848
    Abstract: The present invention provides methods of identifying a genetic abnormality such as mutation in EGFR or KRAS or ALK which is associated with the management of lung cancer or diagnosing, prognosing or monitoring the treatment of pre-cancerous conditions of the lung, such as bronchial dysplasia or atypical alveolar hyperplasia (AAH), through the detection of at least one volatile organic compound indicative of these states.
    Type: Grant
    Filed: August 17, 2011
    Date of Patent: April 19, 2016
    Inventors: Hossam Haick, Nir Peled
  • Patent number: 9304139
    Abstract: An object of the present invention is to provide a method for analyzing hemoglobins which can accurately separate hemoglobins in a short time by liquid chromatography. The method for analyzing hemoglobins by liquid chromatography includes pretreating a sample with an oxidant and a binder for trivalent heme iron.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: April 5, 2016
    Inventors: Hiroaki Taira, Takayuki Oka
  • Patent number: 9278352
    Abstract: A microfluidic arrangement including a substrate in which a microfluidic structure having several adjacent channels and at least one common supply line, into which the adjacent channels merge, is formed. Each of the adjacent channels form the cylinder of a cylinder-piston arrangement for receiving an associated piston. A method for producing the type of microfluidic-arrangement is also disclosed.
    Type: Grant
    Filed: September 27, 2011
    Date of Patent: March 8, 2016
    Inventors: Klaus-Stefan Drese, Daniel Latta, Marion Ritzi-Lehnert, Tina Roeser
  • Patent number: 9234238
    Abstract: Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel.
    Type: Grant
    Filed: April 26, 2011
    Date of Patent: January 12, 2016
    Inventor: Peixuan Guo
  • Patent number: 9222934
    Abstract: The present invention provides a reagent for analysis of immature leukocytes comprising: a surfactant which can damage cell membranes of erythrocytes and mature leukocytes, a solubilizing agent which can shrink the damaged blood cells and at least one dye for staining nucleic acid selected from the dyes of the formulae (I) and (II), wherein X? is an anion.
    Type: Grant
    Filed: August 1, 2013
    Date of Patent: December 29, 2015
    Inventors: Yukiko Kataoka, Tomohiro Tsuji, Shinichiro Oguni, Ayumu Yoshida, Masaki Abe
  • Patent number: 9196036
    Abstract: A device for determining objects in a color recording has an identifier, a feature extractor and a classifier. The identifier is implemented to identify the connected regions whose size or shape correspond to a predetermined condition from a plurality of connected regions existing in a binary image derived from a color recording based on a size or a shape of these connected regions. The feature extractor is implemented, for each of the identified connected regions, to extract a feature set from the color recording. The classifier is implemented to classify the identified connected regions into at least two disjunct groups based on the extracted feature sets for the identified connected regions.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: November 24, 2015
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
    Inventors: Matthias Elter, Thorsten Zerfass, Dirk Steckhan, Thomas Wittenberg, Christian Muenzenmayer, Timo Schlarb