Fluorescent Dyes (e.g., Rhodamine, Etc.) Patents (Class 436/800)
  • Patent number: 9289515
    Abstract: Multivalent fluorescent probes and methods of using these multivalent fluorescent probes for in vitro and in vivo imaging are described.
    Type: Grant
    Filed: April 6, 2015
    Date of Patent: March 22, 2016
    Assignee: Caliper Life Sciences
    Inventors: Rajendra Singh, Ning Zhang, Jae Beom Kim, Zhenjun Diwu
  • Patent number: 8951938
    Abstract: The present invention relates to the use of rosamine derivative compounds, as described herein, in detecting differentiated forms of a cell type of interest in a sample and in screening for compounds which inhibit differentiation of the cell type of interest.
    Type: Grant
    Filed: December 22, 2008
    Date of Patent: February 10, 2015
    Assignees: New York University, President and Fellows of Harvard College
    Inventors: Young-Tae Chang, Young-Hoon Ahn, Yun Kyung Kim, Bridget Wagner, Hyman A. Carrinski, Paul Clemons, Stuart Schreiber
  • Patent number: 8932881
    Abstract: New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent, an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: January 13, 2015
    Inventors: Brij Pal Giri, Dinesh Dagli, Pritam Singh
  • Patent number: 8822722
    Abstract: To provide a fluorescent hydrogel having superior detectability of saccharides such as glucose and minimal invasiveness, a method for producing the same, and a sensor for measuring saccharides using the same. A florescent hydrogel having a structure represented by the following chemical formula 1, a method for producing the same, and a sensor for measuring saccharides using the same.
    Type: Grant
    Filed: September 7, 2011
    Date of Patent: September 2, 2014
    Assignees: Terumo Kabushiki Kaisha, The University of Tokyo
    Inventors: Yun-Jung Heo, Hideaki Shibata, Tetsuro Kawanishi, Shoji Takeuchi, Yukiko Matsunaga, Teru Okitsu
  • Patent number: 8822233
    Abstract: New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent. an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.
    Type: Grant
    Filed: August 23, 2010
    Date of Patent: September 2, 2014
    Inventors: Brij Pal Giri, Dinesh Dagli, Pritam Singh
  • Patent number: 8754244
    Abstract: The present invention relates to new rhodamine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: June 17, 2014
    Assignee: Illumina Cambridge Limited
    Inventors: Nikolai Nikolaevich Romanov, Xiaohai Liu
  • Patent number: 8729267
    Abstract: The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: May 20, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Ronald Graham, Ruiming Zou, Krishna G. Upadhya, Scott C. Benson
  • Patent number: 8710244
    Abstract: Disclosed is a dye having the chemical formula: or a mixture thereof. Each R1 is independently selected from hydrogen, halogen, cyano, alkyl, alkenyl, alkynyl, alkoxyl, alkylthio, alkylamino, dialkylamino, alkylaminocarbonyl, dialkylaminocarbonyl, aryl, heteroaryl, phosphoric acid, or sulfonic acid. Each R2 is independently selected from Each n is independently selected from an integer of 2 to 10.
    Type: Grant
    Filed: December 27, 2012
    Date of Patent: April 29, 2014
    Assignee: Industrial Technology Research Institute
    Inventor: Shin-Shin Wang
  • Patent number: 8703441
    Abstract: The present invention provides and includes monoclonal antibodies (MoAbs or mAbs) specific or preferentially selective for PCBP-1 antigens, hybridoma lines that secrete these PCBP-1 antibodies or antibody fragments, and the use of such antibodies and antibody fragments to detect PCBP-1 antigens, particularly those expressed by cancer cells. The present invention also includes antibodies that are specific for or show preferential binding to a soluble form of PCBP-1. The present invention further includes chimeric and humanized antibodies, processes for producing monoclonal, chimeric, and humanized antibodies using recombinant DNA technology, and their therapeutic uses, particularly in the treatment or diagnosis of cancer progression. The present invention further includes methods and kits for the immunodetection and immunotherapy of cells for samples which express PCBP-1 antigens.
    Type: Grant
    Filed: January 11, 2013
    Date of Patent: April 22, 2014
    Assignee: Alper Biotech, LLC
    Inventor: Özge Alper
  • Patent number: 8673643
    Abstract: A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided.
    Type: Grant
    Filed: November 30, 2010
    Date of Patent: March 18, 2014
    Assignee: General Electric Company
    Inventors: Alex David Corwin, Robert John Filkins, Jun Xie, Christopher James Sevinsky, Kashan Ali Shaikh
  • Patent number: 8647889
    Abstract: The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.
    Type: Grant
    Filed: November 14, 2012
    Date of Patent: February 11, 2014
    Assignee: Access Medical Systems, Ltd.
    Inventors: Robert F. Zuk, Hong Tan
  • Patent number: 8647883
    Abstract: A method of quantitatively measuring the concentration of a chemical species in a sample solution with a sensor film. A hydrogel sensor film is prepared having a chemical composition comprising an indicator that changes its optical property in the ultra-violet, visible or near-infrared spectral range upon being exposed to the chemical species in the sample solution. The film is exposed to a fixed amount of the sample solution. The concentration of the chemical species in the sample solution is quantified using the average absorbance measured from the sensor film.
    Type: Grant
    Filed: September 13, 2010
    Date of Patent: February 11, 2014
    Assignee: General Electric Company
    Inventors: Radislav A. Potyrailo, Timothy M. Sivavec, Caibin Xiao, Theodore J. Cecconie, Lamyaa Hassib, Andrew M. Leach, David B. Engel
  • Patent number: 8628974
    Abstract: A fast and sensitive method and device for protein sequencing are disclosed. The method uses a combination of Edman degradation chemistry and mass spectrometry to sequence proteins and polypeptides. A peptide degradation reaction is performed on a polypeptide or protein ion reactant in the gas phase. The reaction yields a first ion product corresponding to a first amino acid residue of the polypeptide or protein reactant and a polypeptide or protein fragment ion. The mass-to-charge ratio for the first ion product, or the polypeptide or protein fragment ion, or both, is then determined. The first amino acid residue of the polypeptide or protein reactant is then identified from the mass-to-charge ratio so determined.
    Type: Grant
    Filed: July 15, 2005
    Date of Patent: January 14, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Xiaoyu Chen, Michael S. Westphall, Lloyd M. Smith, Brian L. Frey
  • Patent number: 8476075
    Abstract: A method of estimating the amount of unreacted starting materials (glycerides, methyl esters, etc.) and the composition of a biodiesel using TLC in conjunction with a lipophilic dye, Nile Red is described herein. The dye based TLC method of the present invention is convenient and provides significant advantages over existing methods for estimating the purity of a biodiesel composition.
    Type: Grant
    Filed: April 6, 2010
    Date of Patent: July 2, 2013
    Assignee: Board of Regents, The University of Texas System
    Inventors: Eva Deemer, Wen Yee Lee, Xiomara Carolina Chianelli
  • Patent number: 8454895
    Abstract: This invention refers to continuous flow devices for detecting and/or removal of contaminants from a liquid stream. The device comprises a cartridge with an inlet and outlet for the liquid stream, a radiation incident and emerging wall portion and optically transparent support material packed in the cartridge such that a liquid stream can pass between voids or spaces formed within the optically transparent material, as well as a radiation source and detector. The support material comprises molecules for capturing at least one contaminant on the surface of the support material and each of the capture molecules comprises at least one reporter group which emits a signal upon binding of the contaminant. Another form of the invention refers to a rotatable support within the liquid stream.
    Type: Grant
    Filed: May 2, 2008
    Date of Patent: June 4, 2013
    Assignee: Nanyang Technological University
    Inventors: Karina Yew-Hoong Gin, Trevor R. Garrett
  • Patent number: 8435800
    Abstract: The present invention relates in general to labeling reagents useful for labeling biomolecules. In particular, the invention provides activated labeling reagents having the formula L-Ph, wherein L is an activated labeling molecule and Ph is a phenol. The invention further provides methods of preparing the labeling reagents, methods of using the labeling reagents for synthesizing a labeled biomolecule, kits that include reagents for labeling a biomolecule and kits containing labeled biomolecules.
    Type: Grant
    Filed: November 22, 2005
    Date of Patent: May 7, 2013
    Assignee: BioSight Ltd.
    Inventor: Stela Gengrinovitch
  • Patent number: 8431410
    Abstract: A reagent for detecting an analyte by a redox reaction and a fluorimetric determination is disclosed. The reagent comprises a compound of formula (I): wherein R1 and R2 are each independently selected from R, (CH2CH2O)mR, COR, COOR, and OCOR; each R3 is independently selected from NO2, CN, R, OR, OCOR, COOR, SR, and halogen; R is H or C1-C4 alkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR2, COOR, CONR2, SO3R and salts thereof, and PO(OR)3 and salts thereof; m is an integer from 1 to 20; and n is 1, 2, or 3. The reagent may further comprise an enzyme or a coenzyme for reducing or oxidizing an analyte.
    Type: Grant
    Filed: April 9, 2010
    Date of Patent: April 30, 2013
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Carina Horn, Joachim Hoenes, Jürgen Spinke
  • Patent number: 8409877
    Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
    Type: Grant
    Filed: December 29, 2006
    Date of Patent: April 2, 2013
    Assignee: Intel Corporation
    Inventors: David J. Liu, Kai Wu, Xing Su
  • Patent number: 8357801
    Abstract: The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.
    Type: Grant
    Filed: December 4, 2008
    Date of Patent: January 22, 2013
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Praveen Pande, Zaiguo Li, Maciej Szczepanik, Wayne F. Patton
  • Patent number: 8304259
    Abstract: A labeling dye of the present invention includes a coloring portion comprising an organic EL-dye, a bonding portion to be bonded with a biomolecule and a spacer portion for linking the coloring portion and the bonding portion. The present invention provides a high incorporation ratio and also high fluorescence intensity in solid state.
    Type: Grant
    Filed: July 28, 2006
    Date of Patent: November 6, 2012
    Inventor: Shinichiro Isobe
  • Patent number: 8283180
    Abstract: Provided is a method of detecting the presence and quantitating the amount of glycogen from a biological sample. This method employs PAS staining with detection in the infrared range.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: October 9, 2012
    Assignee: Amicus Therapeutics, Inc.
    Inventor: Elfrida Benjamin
  • Patent number: 8183375
    Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: May 22, 2012
    Assignee: Life Technologies Corporation
    Inventors: Zhenjun Diwu, Jixiang Liu, Kyle Gee
  • Patent number: 8178360
    Abstract: Novel rhodamine dye compounds, labelled conjugates comprising the dyes are described, together with methods for their use. The dyes and labelled conjugates are useful as molecular probes in a variety of applications, such as in assays involving staining of cells, protein binding, and analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
    Type: Grant
    Filed: May 16, 2007
    Date of Patent: May 15, 2012
    Assignee: Illumina Cambridge Limited
    Inventors: Colin Barnes, Nikolai Nikolaevich Romanov
  • Patent number: 8173698
    Abstract: The present invention provides fluorogenic probes and corresponding fluorescent compounds, methods of using the probes, compounds and kits that include the probes.
    Type: Grant
    Filed: February 29, 2008
    Date of Patent: May 8, 2012
    Assignee: Life Technologies Corporation
    Inventors: Iain Johnson, Jixiang Liu, Brian Patch, Wai-Yee Leung
  • Patent number: 8173445
    Abstract: The present invention provides compositions and methods for detecting incorporation of a labeled nucleotide triphosphate onto the growing end of a primer nucleic acid molecule. The method is used, for example, to genotype and sequence a nucleic acid. In a preferred embodiment, the method described herein detects individual NTP molecules.
    Type: Grant
    Filed: October 31, 2008
    Date of Patent: May 8, 2012
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: John G. K. Williams, Daniel R. Draney
  • Patent number: 8153446
    Abstract: Fluorophores derived from photoactivatable azide-pi-acceptor fluorogens or from a thermal reaction of an azide-pi-acceptor fluorogen with an alkene or alkyne are disclosed. Fluorophores derived from a thermal reaction of an alkyne-pi-acceptor fluorogen with an azide are also disclosed. The fluorophores can readily be activated by light and can be used to label a biomolecule and imaged on a single-molecule level in living cells.
    Type: Grant
    Filed: May 13, 2009
    Date of Patent: April 10, 2012
    Assignees: Kent State University, Leland Stanford Junior University
    Inventors: Robert J. Twieg, William E. Moerner, Samuel J. Lord, Na Liu, Reichel Samuel
  • Patent number: 8134017
    Abstract: A compound represented by the following formula (1) or (2):
    Type: Grant
    Filed: December 2, 2010
    Date of Patent: March 13, 2012
    Assignee: Riken
    Inventors: Shinichiro Kamino, Shuichi Enomoto
  • Patent number: 8124746
    Abstract: Extended rhodamine compounds exhibiting favorable fluorescence characteristics having the structure are disclosed. In addition, novel intermediates for synthesis of these dyes are disclosed, such intermediates having the structure In addition, methods of making and using the dyes as fluorescent labels are disclosed.
    Type: Grant
    Filed: July 29, 2010
    Date of Patent: February 28, 2012
    Assignee: Applied Biosystems, LLC
    Inventors: Joe Y. L. Lam, Scott C. Benson, Steven M. Menchen
  • Patent number: 8080235
    Abstract: Protein kinase A reporters useful for obtaining measurements of protein kinase A activity with high spatial and temporal resolution can be used in high throughput assays to identify potentially therapeutic compounds.
    Type: Grant
    Filed: October 26, 2006
    Date of Patent: December 20, 2011
    Assignee: The Johns Hopkins University
    Inventors: Jin Zhang, Qiang Ni, Michael David Allen
  • Patent number: 8067602
    Abstract: Asymmetric cyanine fluorescent dyes are represented by general formula I. These kinds of dyes may be used as a staining agent for nucleic acids, with the spectra at 600-900 nm in the near-infrared region and without interference from background fluorescence. These kinds of dyes may be useful with small-type red semiconductor lasers as the light source (such as 633 nm). Compositions comprising these dyes and methods for staining biological samples using these dyes or compositions are also provided.
    Type: Grant
    Filed: December 12, 2008
    Date of Patent: November 29, 2011
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventor: Jianhui Shao
  • Patent number: 8062897
    Abstract: The present invention relates to a method for detecting one or more nascent RNAs in a tissue sample using FISH. In the method, a plurality of probes (8 to 82) may be used to detect a single species of nascent RNA. Further, a plurality of nascent RNA species may be detected simultaneously using between 8 and 82 probes for each nascent RNA. The invention comprises, in addition, methods of preparing a sample for nascent RNA detection by reducing autofluorescence of the tissue sample. These techniques may be synergistically combined to achieve significantly improved results.
    Type: Grant
    Filed: April 14, 2006
    Date of Patent: November 22, 2011
    Assignee: Aureon Laboratories, Inc.
    Inventors: Paola Capodieci, Michael J. Donovan
  • Patent number: 8043802
    Abstract: A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions.
    Type: Grant
    Filed: October 29, 2007
    Date of Patent: October 25, 2011
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Yi Lu, Juewen Liu
  • Patent number: 8030096
    Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
    Type: Grant
    Filed: August 12, 2010
    Date of Patent: October 4, 2011
    Assignee: Applied Biosystems LLC
    Inventors: Linda G. Lee, Ronald Graham, Lily Lu, Elana E. Swartzman, William E. Werner
  • Patent number: 8026110
    Abstract: The present invention relates to a rosamine derivative compound, as described herein, having the following structure: Also disclosed are methods of making such compounds and for using them for detection and imaging.
    Type: Grant
    Filed: September 11, 2007
    Date of Patent: September 27, 2011
    Assignee: New York University
    Inventors: Young-Tae Chang, Young-Hoon Ahn
  • Patent number: 8021888
    Abstract: Disclosed is a method for performing nucleic acid hybridization assays which involve the application of acoustic surface waves. The hybridization assays may be used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or associated with predisposition to various diseases. In a particular aspect, the present method relates to the use of rapid nucleic acid hybridization methods, such as comparative genomic hybridization (CGH), for comparing nucleic acid segments of one genome to corresponding nucleic acid segments in another genome(s).
    Type: Grant
    Filed: January 26, 2006
    Date of Patent: September 20, 2011
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Mansoor S. Mohammed, Natasa Dzidic, Christopher McCaskill
  • Patent number: 7985602
    Abstract: The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.
    Type: Grant
    Filed: August 14, 2007
    Date of Patent: July 26, 2011
    Assignee: Applied Biosystems, LLC
    Inventors: Ronald J. Graham, Ruiming Zou, Krishna G. Upadhya, Scott C. Benson
  • Patent number: 7977120
    Abstract: The invention provides compounds of formulas I, II, and III, methods of making them, and methods of their use. The compounds of the invention can be used as fluorescent sensors, for example, to detect an amine-containing analyte in a biological sample. The compounds can be selective for one type of amine over others and the amount of fluorescence can be correlated with the concentration of the amine in the sample.
    Type: Grant
    Filed: July 28, 2006
    Date of Patent: July 12, 2011
    Assignee: The Curators of the University of Missouri
    Inventors: Timothy Glass, Kristen Secor
  • Patent number: 7960099
    Abstract: The present disclosure provides a white blood cell differentiation reagent comprising a fluorescent dye compound of Formula I, wherein R1, R2, R3, R4, Y? and n are as defined in the specification. The present disclosure also provides a white blood cell detection kit comprising the white blood cell differentiation reagent, and a method of differentiating white blood cells using the white blood cell differentiation reagent.
    Type: Grant
    Filed: December 31, 2007
    Date of Patent: June 14, 2011
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bing Xu, Baohua Zhang, Yuji Kuang
  • Patent number: 7960105
    Abstract: Methods are provided for tagging, characterizing and sorting double-stranded biomolecules while maintaining the integrity of the biomolecules.
    Type: Grant
    Filed: November 29, 2006
    Date of Patent: June 14, 2011
    Assignees: National Institutes of Health, The United States of America as represented by the Department of Health and Human Services, U.S. Government NIH Division of Extramural Inventions and Technology Resources
    Inventors: David C. Schwartz, Kyubong Jo, Dalia M. Dhingra
  • Patent number: 7943307
    Abstract: The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.
    Type: Grant
    Filed: January 19, 2006
    Date of Patent: May 17, 2011
    Assignee: Cornell Research Foundation
    Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
  • Patent number: 7939330
    Abstract: A fluorescent probe which specifically and efficiently traps nitrogen monoxide, zinc ion etc. to emit fluorescence is provided. A compound represented by the following general formula (I): [wherein R1 and R2 represent hydrogen atom, or a group represented by the following formula (A): (wherein X1 to X4 represent hydrogen atom, an alkyl group, or a protective group for amino group, and m and n represent 0 or 1); R3 and R4 represent hydrogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group; R5 to R12 represent hydrogen atom, sulfo group, phospho group, a halogen atom, or a C1-6 alkyl group; R13 and R14 represent a C1-18 alkyl group; Z1 represents oxygen atom, sulfur atom, or —N(R15)— (wherein R15 represents hydrogen atom, or a C1-6 alkyl group); Y1 and Y2 represent —C(?O)—, —C(?S)—, or —C(R16)(R17) (wherein R16 and R17 represent a C1-6 alkyl group); and M? represents a counter ion in a number required for neutralizing the charge].
    Type: Grant
    Filed: October 25, 2010
    Date of Patent: May 10, 2011
    Inventors: Tetsuo Nagano, Eita Sasaki, Hirotatsu Kojima, Tomoya Hirano, Kazuya Kikuchi
  • Patent number: 7888136
    Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties.
    Type: Grant
    Filed: April 7, 2008
    Date of Patent: February 15, 2011
    Assignee: Life Technologies Corporation
    Inventors: Stephen Yue, Ching-Ying Cheung
  • Patent number: 7875467
    Abstract: Chemiluminescent acridinium compounds are used in homogeneous assays to determine the concentration of an analyte in a sample without strong acid or strong base treatment. The chemiluminescent acridinium compounds include acridinium esters with electron donating functional groups at the C2 and/or C7 position on the acridinium nucleus to inhibit pseudo-base formation, or acridinium sulfonamides with or without electron donating functional groups at the C2 and/or C7 position on the acridinium nucleus.
    Type: Grant
    Filed: November 21, 2007
    Date of Patent: January 25, 2011
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Anand A. Natrajan, Todd Sells, Hartmut Schroeder, Guohan Yang, David Sharpe, Qingping Jiang, Hana Lukinsky, Say-Jong Law
  • Patent number: 7871773
    Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: January 18, 2011
    Assignee: Life Technologies Corporation
    Inventors: Richard Haugland, Stephen Yue
  • Patent number: 7867726
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: January 11, 2011
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Dieter Klaubert, Georgyi V. Los, Robert F. Bulleit, Mark McDougall, Chad Zimprich
  • Patent number: 7842511
    Abstract: The present invention relates to the a new protecting group, 4-(O-nitrophthalimido)butyric acid (NPB) that may be attached to a monomer unit during oligosaccharide synthesis such that upon cleavage of this molecule, the solution turns orange. The orange color allows the simple colorimetric monitoring of the completion of the cleavage reaction and, upon collection of the colored fraction, quantification of the amount of the monomer unit that was successfully coupled to the solid phase.
    Type: Grant
    Filed: April 13, 2006
    Date of Patent: November 30, 2010
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Nicola Lucia Pohl, Kwang-Seuk Ko
  • Patent number: 7842512
    Abstract: A method for photochemical reactor characterization includes an application of using dyed microspheres exposed to UV irradiation under a collimated-beam system. Particle specific fluorescence intensity measurements are conducted using samples form the collimated beam and flow-through reactor results using flow cytometry. A numerical model may be used to simulate the behavior of the reactor system to provide a particle-tracking algorithm to interrogate the flow and intensity field simulations for purposes of developing a particle specific estimate of the dose delivery. A method for measuring UV dose distribution delivery in photochemical reactors is provided that includes introducing microspheres labeled with a photochemically-active compound in a UV reactor. The labeled microspheres are harvested downstream of the irradiated zone of a UV reactor and exposed to UV irradiation under a collimated beam of UV irradiation.
    Type: Grant
    Filed: March 26, 2005
    Date of Patent: November 30, 2010
    Assignee: Purdue Research Foundation
    Inventors: Ernest R. Blatchley, III, Chengyue Shen, Zorana Naunovic, Lian-Shin Lin, Dennis A. Lyn, Donald E. Bergstrom, Shiyue Fang, Yousheng Guan, Joseph Paul Robinson, Kathyrn E. Ragheb, Gerald J. Gregori
  • Patent number: 7816519
    Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
    Type: Grant
    Filed: November 16, 2006
    Date of Patent: October 19, 2010
    Assignee: Life Technologies Corporation
    Inventors: Zhenjun Diwu, Jixiang Liu, Kyle Gee
  • Patent number: 7807402
    Abstract: A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation.
    Type: Grant
    Filed: April 22, 2008
    Date of Patent: October 5, 2010
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Carina Horn, Hans-Peter Josel, Juergen Spinke, Rupert Herrmann, Dieter Heindl
  • Patent number: 7807473
    Abstract: A method of quantitatively measuring the concentration of a chemical species in a sample solution with a sensor film. A hydrogel sensor film is prepared having a chemical composition comprising an indicator that changes its optical property in the ultra-violet, visible or near-infrared spectral range upon being exposed to the chemical species in the sample solution. The film is exposed to a fixed amount of the sample solution. The concentration of the chemical species in the sample solution is quantified using the average absorbance measured from the sensor film.
    Type: Grant
    Filed: October 26, 2005
    Date of Patent: October 5, 2010
    Assignee: General Electric Company
    Inventors: Radislav A. Potyrailo, Timothy M. Sivavec, Caibin Xiao, Theodore J. Cecconie, Lamyaa Hassib, Andrew M. Leach, David B. Engel