Immunogenic Carrier Or Carrier Per Se Patents (Class 436/823)
  • Patent number: 9982250
    Abstract: Some embodiments described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: May 29, 2018
    Assignee: Illumina Cambridge Limited
    Inventors: Xavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown
  • Patent number: 8299222
    Abstract: The invention generally relates to hapten compounds comprising either (+) methamphetamine or (+) amphetamine conjugated to a linker. Generally speaking, hapten compounds of the invention may be used to elicit an immune response to one or more of (+) methamphetamine, (+) amphetamine, or (+) MDMA.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: October 30, 2012
    Assignee: The Board of Trustees of the University of Arkansas
    Inventors: Samuel M. Owens, Frank Ivy Carroll, Philip Abraham
  • Patent number: 8202734
    Abstract: A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: June 19, 2012
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer
  • Patent number: 8178359
    Abstract: A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: May 15, 2012
    Assignee: Bayer Healthcare AG
    Inventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
  • Patent number: 8133718
    Abstract: An analytical strip and a detecting method using the analytical strip are provided. The analytical strip includes a substrate having a channel thereon. The channel has a first region, a second region and a third region, which are arranged successively. A first antibody is localized in the first region. A saccharide and a peroxidase are localized in the first or second region. A second antibody for recognizing a different epitope of an identical antigen with the first antibody is immobilized in the second region. A substrate reagent including a saccharide oxidase is localized in the third region.
    Type: Grant
    Filed: April 29, 2009
    Date of Patent: March 13, 2012
    Assignee: Actherm Inc
    Inventors: Yi-Jen Wu, Chih-Wei Hsieh, Wen-Pin Hsieh
  • Patent number: 8030091
    Abstract: A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: October 4, 2011
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer, Paul Lambotte, Paul J. Lawrence
  • Patent number: 7964370
    Abstract: An analytical strip and a detecting method using the analytical strip are provided. The analytical strip includes a substrate having a channel thereon. The channel has a first region, a second region and a third region, which are connected sequentially. A first antibody is localized in the first region. A saccharide and a peroxidase are localized in the first or second region. A second antibody for recognizing a different epitope of an identical antigen with the first antibody is immobilized in the second region. An optical substrate and a substrate reagent including a saccharide oxidase are localized in the third region.
    Type: Grant
    Filed: October 17, 2008
    Date of Patent: June 21, 2011
    Assignee: Actherm Inc
    Inventors: Yi-Jen Wu, Wen-Pin Hsieh, Chih-Wei Hsieh
  • Patent number: 7858396
    Abstract: A lateral flow test strip for the analysis of a sample featuring numerous capture zones arranged in an array such that each capture zone is substantially equidistant from the sample containing area. The sample does not pass through another capture zone to reach any one of the other capture zones. The capture zones are preferably arranged in a linear array perpendicular to the flow of the sample through the lateral flow test strip. The lateral flow test strip allows for an increased number of simultaneous analyses of numerous analytes from one sample to occur on one lateral flow test strip.
    Type: Grant
    Filed: October 31, 2006
    Date of Patent: December 28, 2010
    Assignee: Orasure Technologies, Inc.
    Inventors: Paul L.A.M. Corstjens, Keith Kardos, R. Sam Niedbala, Hans J. Tanke, Michel Zuiderwijk, Hans H. Feindt, Vijay K. Mokkapati, Jess Aaron Kimball
  • Patent number: 7713746
    Abstract: The invention provides a dipstick and a kit comprising the dipstick, for testing for the presence of a plurality of different targets in a sample solution which comprises: a dipstick having a plurality of different capture zones and, immobilised to each capture zone, a different capture moiety, each capture moiety capable of capturing a different target; and, separately, a plurality of different detection probes, each detection probe capable of binding to a different target and each detection probe being labelled with or enabling the formation of a detection signal so that the presence of each target is indicated by the formation of a signal at the capture zone for that target; wherein the target for at least two of the capture moieties is a disease causing micro-organism or a marker indicating the existence of a disease, disorder, or condition of the host from which the sample solution was derived, and wherein at least two of the capture moieties are capable of binding to different components or markers of t
    Type: Grant
    Filed: October 15, 2001
    Date of Patent: May 11, 2010
    Assignee: Diagnostics for the Real World, Ltd.
    Inventors: Helen Lee, Magda Anastassova Dineva
  • Patent number: 7704753
    Abstract: The present invention provides devices, methods and kits for detecting the presence of an analyte in a liquid sample and indicating to the user the presence or absence of the analyte through the formation of a recognizable symbol during the assay. In one embodiment, the present invention provides test strips having a matrix including a sample application zone, a reagent zone, and a detection zone. Within the detection zone are located positive and negative control areas, and an analyte binding area. The positive control area contains one or more components that exhibit a first color when dry and a second color when wetted. When the assay is conducted, the positive control area is wetted by the assay fluids and turns to a second color, thereby functioning as a positive procedural control. When analyte is present in the sample, it binds at the analyte binding area, which interacts with the positive control area to form a second recognizable symbol at the detection zone.
    Type: Grant
    Filed: March 3, 2005
    Date of Patent: April 27, 2010
    Assignee: Inverness Medical Switzerland GmbH
    Inventors: Zuifen Tang, Dengfeng Xiong, Shujiang Wu, Zhumin Guan
  • Patent number: 7659053
    Abstract: The present invention provides a series of methods, compositions, and articles for altering a property of a surface (for example, the cytophilicity and/or the hydrophilicity), by exposing at least a portion of the surface to a non-chemical, force-creating field and/or force, such as an electric field. The field/force may be created by any suitable technique. For instance, the field can be created by applying a voltage across the surface, by electrical induction, etc. In certain embodiments, the surface includes molecules attached thereto that can be detached when exposed to non-chemical, force-creating fields and/or forces, thereby altering the chemical composition of at least a portion of the surface. In one set of embodiments, the molecules attached to the surface may include molecules forming a self-assembled monolayer on the surface. In some embodiments, the molecules attached to the surface may include thiol moieties (e.g., as in an alkanethiol), by which the molecule can become attached to the surface.
    Type: Grant
    Filed: July 14, 2005
    Date of Patent: February 9, 2010
    Assignee: President and Fellows of Harvard College
    Inventors: Xingyu Jiang, Rosaria Ferrigno, George M. Whitesides
  • Patent number: 7645614
    Abstract: The present invention provides a method of analyzing the specific interaction between a molecule to be analyzed and a molecule that specifically interacts with the former molecule on a solid phase using a molecule-immobilized solid phase support mixture prepared by binding the subject molecule to the solid phase support without specifying the binding position on the molecule side, particularly a method wherein the immobilization is conducted via a spacer introduced to the molecule without specifying the binding position on the molecule side, which method makes it possible to identify and select only a molecule that exhibits a specific interaction with a molecule to be analyzed, without an investigation of structure-activity correlation, which has conventionally been essential, and hence enables an analysis of the interaction between these molecules.
    Type: Grant
    Filed: October 31, 2003
    Date of Patent: January 12, 2010
    Assignee: Reverse Proteomics Research Institute Co., Ltd.
    Inventors: Akito Tanaka, Tomohiro Terada, Akira Yamazaki, Tsuruki Tamura, Hidenori Nakajima
  • Patent number: 7632929
    Abstract: The invention generally relates to hapten compounds comprising either (+) methamphetamine or (+) amphetamine conjugated to a linker. Generally speaking, hapten compounds of the invention may be used to elicit an immune response to one or more of (+) methamphetamine, (+) amphetamine, or (+) MDMA.
    Type: Grant
    Filed: April 9, 2007
    Date of Patent: December 15, 2009
    Assignee: The Board of Trustees of the University of Arkansas
    Inventors: Samuel M. Owens, Frank Ivy Carroll, Philip Abraham
  • Patent number: 7632687
    Abstract: The invention relates to devices for performing single step assays for the determination of the presence or absence of an analyte in a liquid sample, and methods of determining the presence or absence of such analytes using such devices. Devices disclosed comprise a labeled analyte-binding reagent reversibly-immobilized on a non-porous solid material, which solid material is in physical contact with a dry porous carrier bearing an immobilized analyte-binding reagent. Also provided are quantitative assay devices.
    Type: Grant
    Filed: March 23, 2005
    Date of Patent: December 15, 2009
    Assignee: Quidel Corporation
    Inventor: Cem Gokhan
  • Patent number: 7615376
    Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: November 10, 2009
    Assignee: Bayer Schering Pharma Aktiengesellschaft
    Inventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
  • Patent number: 7553675
    Abstract: A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: January 11, 2007
    Date of Patent: June 30, 2009
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer, Paul Lambotte, Paul J. Lawrence
  • Patent number: 7537937
    Abstract: A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: May 26, 2009
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer
  • Patent number: 7521259
    Abstract: In one aspect, an assay test strip includes a test label that specifically binds a target analyte and a control label that is free of any specific binding affinity for the target analyte and has a different optical characteristic than the test label. In another aspect, an assay test strip includes a test label that specifically binds a target analyte and at least one non-specific-binding label that is free of any specific binding affinity for the target analyte. Systems and methods of reading assay test strips also are described.
    Type: Grant
    Filed: September 26, 2005
    Date of Patent: April 21, 2009
    Inventors: Patrick T. Petruno, John F. Petrilla, Michael J. Brosnan, Rong Zhou, Daniel B. Roitman
  • Patent number: 7521260
    Abstract: An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.
    Type: Grant
    Filed: November 16, 2005
    Date of Patent: April 21, 2009
    Inventors: Patrick T. Petruno, John F. Petrilla, Michael J. Brosnan, Rong Zhou, Daniel B. Roitman, Bo U. Curry
  • Patent number: 7371829
    Abstract: The invention provides haptens, immunogens comprising such haptens coupled to an antigenicity-conferring carrier material, conjugates comprising such haptens bonded to a labelling agent as well as, antibodies raised against such immunogens and capable of binding with ketamine and its primary metabolite, norketamine.
    Type: Grant
    Filed: January 31, 2003
    Date of Patent: May 13, 2008
    Assignee: Randox Laboratories Limited
    Inventors: Robert Ivan McConnell, Elouard Benchikh, Stephen Peter Fitzgerald, John Victor Lamont
  • Patent number: 7368296
    Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support.
    Type: Grant
    Filed: January 17, 2002
    Date of Patent: May 6, 2008
  • Patent number: 7312028
    Abstract: A simple easy to manufacture analytical device capable of performing membrane based immunoassays on batch of samples within 3 to 10 minutes wherein the method permits focused application of samples, costly labeled immunoassay and signal amplification reagents, said device includes an antibody-immobilized micro porous membrane, breadth corner layer of which is directly attached to a semi-rigid liquid-impervious body with water insoluble adhesive; absorbent body is provided separately and is not attached to analytical device during manufacture, absorbent body is wetted and is placed proximal to the lower surface of the membrane thereby forming networks of capillary channels with the absorbent body; flow of samples or reagents is always kept downwards and focused without application of any force to the absorbent body and the use of disposable adsorbent body permits stepwise addition of signal amplification reagents for ultra sensitive detection of diagnostically important molecules by visual examination of the m
    Type: Grant
    Filed: July 28, 2006
    Date of Patent: December 25, 2007
    Assignees: Council of Scientific & Industrial Research, Department of Biotechnology, A Department of Government of India
    Inventors: Tarun K. Dhar, Arindam Pal
  • Patent number: 7303925
    Abstract: A visual perception of a colored site in an immunoassay device comprising a strip for enabling capillary migration of a fluid sample therealong, a labeled reagent disposed on the strip and formulated for suspension in the sample migrating therepast and a captive reagent immobilized on the strip in a path of sample migration and formulated to bind to is enhanced by changing a color of the strip to the color which is complimentary to the colored site.
    Type: Grant
    Filed: September 2, 2004
    Date of Patent: December 4, 2007
    Assignee: Varian Inc.
    Inventors: Steven P. Sidewell, Steven S. Bachand
  • Patent number: 7303923
    Abstract: We describe an assay device which comprises (a) a substrate comprising: (i) a porous material capable of chromatographically transporting a liquid and (ii) one or more test reagents for an assay provided on the porous material; and (b) a water-impermeable coating polymer attached to the porous material so as to define a continuous bibulous compartment.
    Type: Grant
    Filed: September 10, 2002
    Date of Patent: December 4, 2007
    Assignee: Diamatrix Limited
    Inventors: David John Hardman, James Howard Slater, Adam G. Reid, William Kenneth Lang, James Richard Jackson
  • Patent number: 7300802
    Abstract: The present invention relates to a biosensor for point-of-care testing (POCT) whose detection sensitivity was remarkably improved by introducing to membrane strip chromatographic assay system a successive cross-flow procedure for immune reaction and enzymatic reaction.
    Type: Grant
    Filed: April 20, 2004
    Date of Patent: November 27, 2007
    Assignee: Biodigit Laboratories Corp.
    Inventors: Se Hwan Paek, Jung Hwan Cho, Ser Ka Kim
  • Patent number: 7297554
    Abstract: A competitive immunoassay, dye and method for rapidly detecting the presence of one or more target ligands within a fluid sample suspected of containing such ligand or ligands. The immunoassay comprises a protein-binding membrane, a first absorbent pad, a second absorbent pad, and a third absorbent pad. The protein-binding membrane has at least two regions of antibodies bound thereto for detecting dissimilar ligands. The second absorbent pad has formed therein a colloidal gold tracer having one, and preferably two or more ligand analog protein complexes adhering thereto. To utilize the system, the immunoassay strip is placed within a fluid sample. To the extent the target ligand is absent, a visual indicator will be provided signaling such absence. To the extent the target ligand is present at or above a threshold level, no such visual signal will be produced.
    Type: Grant
    Filed: October 28, 2003
    Date of Patent: November 20, 2007
    Assignee: Microdiagnostics, Inc.
    Inventors: Roy Chung, June Shozi
  • Patent number: 7288416
    Abstract: An objective of the present invention is to provide ionic polymers that can provide for polymer-comprising substrates whose surfaces can stably and easily immobilize ligands such as sugar chains interacting with biological substances, in which the ligand portion exists stably even in water or buffer, and its detection capability does not decrease for a long period of time; and substrates comprising the polymers. The ionic polymer of this invention comprises two or more ionic functional groups (A) and two or more biointeracting groups represented by formula L-X-B-[wherein, L denotes a ligand capable of interacting with a biological substance, X denotes a spacer, and B denotes a binding group] as side chains covalently bound to a main chain polymer.
    Type: Grant
    Filed: May 21, 2004
    Date of Patent: October 30, 2007
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Hirotaka Uzawa, Norihiko Minoura, Hiroki Itou, Kazuhiro Taguchi
  • Patent number: 7241628
    Abstract: The invention concerns a method for the detection of an analyte in a sample using analyte-specific conjugates which have at least one heterologous group for an analyte-independent binding to a control zone. The present invention additionally provides new conjugates and reagent kits.
    Type: Grant
    Filed: July 1, 2005
    Date of Patent: July 10, 2007
    Assignee: Roche Diagnostics Corporation
    Inventors: Jurgen Schaffler, Barbara Upheimer
  • Patent number: 7226793
    Abstract: A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: June 12, 2003
    Date of Patent: June 5, 2007
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer
  • Patent number: 7217530
    Abstract: A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and the presence of PrP is then detected.
    Type: Grant
    Filed: June 9, 2005
    Date of Patent: May 15, 2007
    Assignees: Agence Francaise de Securite Sanitaire des Aliments-AFSSA, Centre National de la Recherche Scientifique-CNRS, Universite Claude Bernard Lyon and Biomerieux
    Inventors: Aly Moussa, Anthony William Coleman, Patrick Shahgaldian, Eric Da Silva, Ambroise Martin, Adina Nicoleta Lazar, Edwige Leclere, Marilyne Dupin, Herve Perron
  • Patent number: 7208299
    Abstract: The present invention is directed to a composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces and hydrophobic coated surfaces. The composition is a biomolecule conjugated end-group activated polymer (EGAP). The biomolecule conjugated EGAP can be put to numerous uses including cell adhesion, cell growth, cell sorting, and other biological assays.
    Type: Grant
    Filed: December 30, 2003
    Date of Patent: April 24, 2007
    Assignee: Universtiy of Utah Research Foundation
    Inventors: Karin D. Caldwell, Patrick A. Tresco, Jennifer Neff
  • Patent number: 7179657
    Abstract: A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: September 10, 2002
    Date of Patent: February 20, 2007
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer, Paul Lambotte, Paul J. Lawrence
  • Patent number: 7160735
    Abstract: Compositions and methods are disclosed for detecting multiple target analytes in a sample using microparticles having molecular tags attached by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such microparticles under conditions that permit stable complexes to form between binding moieties on the surfaces of the microparticles and the analytes. In one aspect of the invention, the a second binding composition is added so that complexes form among the microparticle-bound binding moieties, the analytes, and second binding moieties of the second binding composition. Such second binding moieties have cleavage-inducing moieties attached that upon activation cause the cleavage of the cleavable linkages and the release of molecular tags. Released molecular tags are separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.
    Type: Grant
    Filed: November 8, 2002
    Date of Patent: January 9, 2007
    Assignee: Monogram Biosciences, Inc.
    Inventors: Peter Dehlinger, Sharat Singh, Hrair Kirakossian
  • Patent number: 7141431
    Abstract: This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.
    Type: Grant
    Filed: June 30, 2005
    Date of Patent: November 28, 2006
    Assignee: Luminex Corporation
    Inventors: Mark B. Chandler, Don J. Chandler, Jason Bedre
  • Patent number: 7138280
    Abstract: In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.
    Type: Grant
    Filed: October 3, 2002
    Date of Patent: November 21, 2006
    Assignee: Bayer Healthcare AG
    Inventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
  • Patent number: 7109309
    Abstract: It is intended to provide an antibody specific to HbA1c, antibody-producing cells capable of supplying the antibody in a stable state in the future, and a method of constructing the antibody-producing cells without any probability factors, and a method which comprises fusing mouse spleen cells, which have been sensitized with an immunogen composed of a compound containing the following structural formula (I) and a binding protein, with a myeloma-origin cell line, obtaining monoclonal antibody-producing cells by cloning, and then purifying and acquiring the monoclonal antibody produced by these cells into the culture supernatant
    Type: Grant
    Filed: July 13, 2001
    Date of Patent: September 19, 2006
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Nobuyuki Shigetoh, Hiroshi Nakayama, Keiko Yugawa, Fumihisa Kitawaki
  • Patent number: 7087389
    Abstract: A simple easy to manufacture analytical device capable of performing membrane based immunoassays on batch of samples within 3 to 10 minutes wherein the method permits focused application of samples, costly labeled immunoassay and signal amplification reagents, said device includes an antibody-immobilized micro porous membrane, breadth corner layer of which is directly attached to a semi-rigid liquid-impervious body with water insoluble adhesive; absorbent body is provided separately and is not attached to analytical device during manufacture, absorbent body is wetted and is placed proximal to the lower surface of the membrane thereby forming networks of capillary channels with the absorbent body; flow of samples or reagents is always kept downwards and focused without application of any force to the absorbent body and the use of disposable adsorbent body permits stepwise addition of signal amplification reagents for ultra sensitive detection of diagnostically important molecules by visual examination of the m
    Type: Grant
    Filed: February 9, 2001
    Date of Patent: August 8, 2006
    Assignees: Council of Scientific & Industrial Research, Department of Biotechnology, A Department of Govenment of India
    Inventors: Tarun K. Dhar, Arindam Pal
  • Patent number: 7008788
    Abstract: Devices and methods are disclosed for containing and processing samples on the surface of supports. Biopolymer features are attached to the surfaces of the supports. A device in accordance with the invention comprises a housing and a support confined by the housing. The housing comprises a well having walls and at least one wall extending from the edge of the well. The height of the walls of the well is at least great enough, and the design of the at least one wall is such, that liquid contained in the well is not drawn out of the well to any substantial degree by surface tension or small movements or small mechanical vibrations. In one embodiment, the at least one wall is designed such that corners thereof are curved or are distant from the edge of the well to substantially eliminate wicking of liquid from the well. Also disclosed are methods for mixing materials on the surface of a support. A sample is incubated with the surface of the support of the aforementioned device.
    Type: Grant
    Filed: July 30, 2001
    Date of Patent: March 7, 2006
    Assignee: Agilent Technologies, Inc.
    Inventors: Donald J. Schremp, Richard O. Hilson, Edward P. Donlon
  • Patent number: 6998246
    Abstract: The invention concerns a method for the detection of an analyte in a sample using analyte-specific conjugates which have at least one heterologous group for an analyte-independent binding to a control zone. The present invention additionally provides new conjugates and reagent kits.
    Type: Grant
    Filed: February 28, 2001
    Date of Patent: February 14, 2006
    Assignee: Roche Diagnostics Corporation
    Inventors: Jürgen Schäffler, Barbara Upmeier
  • Patent number: 6979543
    Abstract: A sensor is provided including a polymer capable of having an alterable measurable property from the group of luminescence and electrical conductivity, the polymer having an intermediate combination of a recognition element, a tethering element and a property-altering element bound thereto and capable of altering the measurable property, the intermediate combination adapted for subsequent separation from the polymer upon exposure to an agent having an affinity for binding to the recognition element whereupon the separation of the intermediate combination from the polymer results in a detectable change in the alterable measurable property, and, detecting said detectable change in the alterable measurable property.
    Type: Grant
    Filed: April 10, 2003
    Date of Patent: December 27, 2005
    Assignee: The Regents of the University of California
    Inventors: Liaohai Chen, Duncan W. McBranch, Hsing-Lin Wang, David G. Whitten
  • Patent number: 6933109
    Abstract: Methods and reagents for rapid purification and/or identification of particles in a liquid sample are described. The technique uses centrifugation to concentrate particles against a slanted surface having an agent specifically binding to the particles. This method is applicable for the rapid identification of viruses and other difficult or impossible to culture microorganisms without replication or amplification of the microorganism.
    Type: Grant
    Filed: December 22, 2000
    Date of Patent: August 23, 2005
    Assignee: Large Scale Proteomics Corporation
    Inventor: Norman G. Anderson
  • Patent number: 6929943
    Abstract: A device for analyzing immunoassays with a liquid assay medium includes a vessel for holding the assay medium. The vessel has a base comprised of a solid body having a first side wall and a top surface forming a boundary surface of the solid body. First reaction agents are dissolved in the assay medium in the vessel and are labeled with a luminophore or different luminophores and second reaction agents are bonded to the boundary surface within a boundary layer of the assay medium. A transmitter for emitting light rays is arranged so that the light rays are coupled into the base of the vessel via the first side wall and conducted at the total reflection angle to the boundary surface so that luminophore-labeled first reaction agents that are bonded to the second reaction agents are optically excited by at least some of the light rays and emit fluorescent and/or phosphorescent rays. A receiver is positioned for quantitatively detecting the fluorescent rays and/or phosphorescent rays.
    Type: Grant
    Filed: August 24, 2000
    Date of Patent: August 16, 2005
    Assignees: Leuze Electronic GmbH & Co., Stiftung fur Diagnostische Forschung
    Inventors: Gerald Quapil, Manfred Schawaller
  • Patent number: 6916661
    Abstract: This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.
    Type: Grant
    Filed: October 28, 2003
    Date of Patent: July 12, 2005
    Assignee: Luminex Corporation
    Inventors: Mark B. Chandler, Don J. Chandler
  • Patent number: 6913936
    Abstract: A novel compound comprising an immunologically invisible polyethylene glycol copolymer is used to carry one or more immunologically reactive substances. The novel compounds may be used as part of kits for immunological assays.
    Type: Grant
    Filed: October 17, 2001
    Date of Patent: July 5, 2005
    Assignee: University of Medicine and Dentistry of New Jersey
    Inventors: Bo Qiu, Guobao Zhang, Stanley Stein, Leonard H Sigal, Michael Brunner, Michael Katz
  • Patent number: 6890764
    Abstract: Described herein are assays and components for encoding and decoding microspheres. Each assay or component described utilizes at least one nanocrystal.
    Type: Grant
    Filed: December 31, 2002
    Date of Patent: May 10, 2005
    Assignee: Illumina, Inc.
    Inventors: Mark S. Chee, Steven M. Barnard, Chanfeng Zhao
  • Patent number: 6884628
    Abstract: Multifunctional, polyionic copolymers with molecular architectures and properties optimized for specific applications are synthesized on/or applied to substrate surfaces for analytical and sensing purposes. The coatings are particularly useful for suppression of non-specific interaction, adsorption or attachment of molecular or ionic components present in an analyte solution. Chemical, biochemical or biological groups that are able to recognize, interact with and bind specifically to target molecules in the material containing the analyte to be detected can be coupled to, integrated into, or absorbed to the multifunctional copolymers. These multifunctional copolymer coatings are compatible with a variety of different established methods to detect, sense and quantify the target molecule in an analyte.
    Type: Grant
    Filed: April 28, 2000
    Date of Patent: April 26, 2005
    Assignees: Eidgenossische Technische Hochschule Zurich, Universitat Zurich
    Inventors: Jeffrey A. Hubbell, Marcus Textor, Donald L. Elbert, Stephanie Finken, Rolf Hofer, Nicholas D. Spencer, Laurence Ruiz-Taylor
  • Patent number: 6884597
    Abstract: A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.
    Type: Grant
    Filed: July 18, 2000
    Date of Patent: April 26, 2005
    Assignee: Medical & Biological Laboratories, Co., Ltd.
    Inventors: Yoichi Taya, Katsuyuki Tamai, Toshiaki Miyazaki
  • Patent number: 6855561
    Abstract: A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.
    Type: Grant
    Filed: September 10, 2001
    Date of Patent: February 15, 2005
    Assignee: Quidel Corporation
    Inventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer
  • Patent number: 6846681
    Abstract: The present invention is directed to a silicon substrate having a monolayer formed by an electrochemically-induced reaction between silicon hydride moieties on the silicon surface and optionally substituted alkynes covalently bound to the surface of the silicon substrate and to a method for electrochemically producing such a functionalized silicon substrate. The method of forming a covalently bound monolayer on a silicon surface comprises the steps of contacting the silicon surface with a C2-C24 alkyne and electrografting optionally substituted alkynes to the silicon surface.
    Type: Grant
    Filed: July 19, 2002
    Date of Patent: January 25, 2005
    Assignee: Purdue Research Foundation
    Inventors: Jillian M. Buriak, Michael P. Stewart, Edward Robins
  • Patent number: 6835552
    Abstract: The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.
    Type: Grant
    Filed: December 14, 2000
    Date of Patent: December 28, 2004
    Assignee: The Regents of the University of California
    Inventors: Robin R. Miles, Kodumudi S. Venkateswaran, Christopher K. Fuller