Immunogenic Carrier Or Carrier Per Se Patents (Class 436/823)
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Patent number: 4543439Abstract: A hybridoma cell line is disclosed that secretes monoclonal antibodies which serve as a high titer, reproducible, biological reagent useful in biological/medical research for isolating and identifying phosphotyrosine-containing proteins. In addition, the antibodies have potential uses in diagnosis of a variety of diseases, including certain cancers. The antibodies, which have demonstrated affinity for a variety of molecules containing o-phosphotyrosine residues, were prepared using a synthetic analog, p-azobenzyl phosphonate (ABP) covalently linked to a carrier protein, as the antigen.Type: GrantFiled: December 13, 1982Date of Patent: September 24, 1985Assignee: Massachusetts Institute of TechnologyInventors: A. Raymond Frackelton, Jr., Herman N. Eisen, Alonzo H. Ross
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Patent number: 4513088Abstract: An assay that facilitates screening of hybridoma culture media for monoclonal anti-idiotype antibodies, particularly murine monoclonal antibodies that are useful for treating human B cell tumors is disclosed. The assay is a solid phase type assay and involves: incubating a lysate of the patienREFERENCE TO GOVERNMENT GRANT OR CONTRACTThe invention described herein was made in the course of work under a grant or contract from the National Institutes of Health.Type: GrantFiled: March 30, 1983Date of Patent: April 23, 1985Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald Levy, David G. Maloney, Kristiaan Thielemans
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Patent number: 4506019Abstract: Novel activated polyamide polymer container or test tube means and a method for the determination of ligand or analyte content in aqueous samples, are provided. The surfaces of the container tubes are specially activated thermally during their manufacture by injection molding thereby avoiding the need for subsequent coating of the tubes, e.g., with antibody, for binding assay procedures.Type: GrantFiled: September 24, 1982Date of Patent: March 19, 1985Assignee: Leeco Diagnostics, Inc.Inventor: Jin P. Lee
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Patent number: 4504585Abstract: An affinity immunoassay system in which a solid phase nonimmunological, group-specific ligand is used to insolubilize the analyte of interest either simultaneously, before or after binding all of the analyte with a labelled monospecific antibody and the concentration of the analyte is then determined by measuring the label activity present in the solid phase in relation to a single point calibrator solution containing a known amount of the analyte substance.Type: GrantFiled: April 5, 1982Date of Patent: March 12, 1985Assignee: Aalto Scientific, Ltd.Inventor: Robert A. Reynolds
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Patent number: 4497899Abstract: The present disclosure relates to a solid phase immunoassay for the detection of Chlamydia trachomatis antigens in a clinical specimen, wherein the Chlamydia trachomatis antigens to be determined are coated or adsorbed on the solid phase.Type: GrantFiled: March 28, 1983Date of Patent: February 5, 1985Assignee: Abbott LaboratoriesInventors: Alan S. Armstrong, John E. Herrmann, Lawrence V. Howard
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Patent number: 4497900Abstract: The present disclosure relates to a solid phase immunoassay for the detection of Neisseria gonorrhoeae antigens in a clinical specimen, wherein the Neisseria gonorrhoeae antigens to be determined are coated or adsorbed on the solid phase.Type: GrantFiled: March 28, 1983Date of Patent: February 5, 1985Assignee: Abbott LaboratoriesInventors: Dinah Abram, Alan S. Armstrong, John E. Herrmann, Chung-Mei Ling
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Patent number: 4495295Abstract: A process for the detection of an antigen or antibody in a specimen which process comprises:(a) contacting said specimen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen or antibody in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate;(b) contacting the washed material of step `a` with a radioactive material labeled or enzyme labeled antibody or antigen, incubating the so-contacted material and washing the same; and(c) effecting radioimmunoassay if said antibody or antigen is radioactive or enzyme labeled immunoassay is said antibody or antigen is enzyme labeled.Type: GrantFiled: March 29, 1982Date of Patent: January 22, 1985Assignee: New York Blood Center, Inc.Inventor: A. Robert Neurath
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Patent number: 4493899Abstract: A method is provided for testing for particular antibodies in the serum of a patient. The antibodies may be those of systemic lupus erythematosus and may constitute IgG and IgM immunoglobulins. The IgG and IgM immunoglobulin may be individually labeled radioactively.An antigen (such as DNA) may be attached to a support such as sepharose. The attachment may be facilitated as by irradiation with ultraviolet light. The DNA may be single stranded or double stranded. When double-stranded DNA is used, single-stranded portions in the double strands may be removed as by a suitable enzyme.The particular antibodies may be attached to the antigen such as the supported DNA. An assay may then be provided to determine the attachment of the particular antibodies to the supported DNA.Type: GrantFiled: October 16, 1981Date of Patent: January 15, 1985Assignee: City of HopeInventors: Lloyd H. Smith, Raymond L. Teplitz
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Patent number: 4492760Abstract: An assay for HLA D phenotype wherein antigen-pulsed monocytes are contacted with antigen-specific T lymphocytes or T cell hybridomas and the extent of selective binding between the pulsed monocytes and the T lymphocytes or T cell hybridomas determines HLA D phenotype of the monocyte. The assay is particularly useful for quickly typing donor tissue prior to transplantation.Type: GrantFiled: April 19, 1983Date of Patent: January 8, 1985Assignee: The Wistar Institute of Anatomy and BiologyInventor: Elaine C. DeFreitas
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Patent number: 4487839Abstract: Methods for determining the presence of antigens or antibodies in an aqueous sample or presence of antigens on the surface of cells. A preferred embodiment employs fluorescent antigens which compete with the sample antigens for antibody binding sites. The antibodies are deposited on a support surface means in alternating patterns. The surface means and fluorescence detector are translocated with respect to each other and a signal generated by the detection of the repeating pattern of fluorescence. The signal is analyzed by means of a gated integrator responsive to a gate track control means also located on the surface means. Immunoassay methods having increased sensitivity are thereby obtained.Type: GrantFiled: January 5, 1983Date of Patent: December 11, 1984Assignee: Ortho Diagnostic Systems Inc.Inventor: Louis A. Kamentsky
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Patent number: 4477576Abstract: A method of determining the concentration of an antigen in a sample solution comprising(a) coating an antigen-protein conjugate onto a solid matrix,(b) conjugating an enzyme to an antibody specific for said antigen,(c) to a known quantity of a solution containing the antibody-enzyme conjugate of (b) adding a specified quantity of a sample containing an unknown amount of the antigen whose content is to be determined,(d) contacting the coated solid matrix of (a) with the solution (c) and incubate so as to effect binding between the antibody and antigen, some of the antigen being that from the sample and some being that on the solid matrix,(e) removing the solid matrix from the solution and washing,(f) immersing the solid matrix in a solution containing a known amount of an enzyme-substrate which is acted upon by the enzyme so as to effect reaction between the enzyme and enzyme-substrate to produce a product, and then separating the solid matrix from the solution of enzyme-substrate, and(g) then measuring the soType: GrantFiled: July 26, 1982Date of Patent: October 16, 1984Assignee: Mex Research AssociatesInventors: Alice Deutsch, Harvey Brandwein, Herbert Platt, Dianne M. Hunter, Andrew Dubitsky, Susan M. Durham
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Patent number: 4461832Abstract: A process for the preparation of an enzymatically active formulation embedded in silica gel, wherein an aqueous mixture of an enzymatically active formulation and a dissolved alkali metal silicate and/or ammonium silicate is suspended in an organic, water-immiscible fluid and then converted to a water-insoluble gel.Type: GrantFiled: May 10, 1982Date of Patent: July 24, 1984Assignee: BASF AktiengesellschaftInventors: Chung-Ji Tschang, Heinrich Klefenz, Axel Sanner, Wolfgang Zahn
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Patent number: 4442218Abstract: A method of measuring the degree of partitioning of a labeled species between free and bound states which involves the use of an insoluble porous monolith having a means for binding a portion of the labeled species within the pores thereof, which monolith is capable of substantially attenuating the signal emitted by labeled species subsequently bound within the pores thereof.Type: GrantFiled: May 27, 1981Date of Patent: April 10, 1984Assignee: Corning Glass WorksInventors: Lynn G. Amos, Charles H. Rogers
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Patent number: 4434150Abstract: An immunological substance detecting reagent is provided which, in the preferred embodiment, employs an immunological homolog specific for the immunological substance to be detected coupled to a water-soluble polymer having a net charge not greater than zero. The water-soluble polymer further has associated a plurality of marker substances such as fluorophore molecules thereby providing a reagent of increased sensitivity for the detection of an antigen-antibody immunological reaction.Type: GrantFiled: October 19, 1981Date of Patent: February 28, 1984Assignee: Ortho Diagnostic Systems, Inc.Inventors: A. R. M. Azad, Stefan J. Kirchanski, Michael C. Brown
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Patent number: 4433059Abstract: Reagents and methods are described for an immunoassay test of increased sensitivity and decreased complexity employing an immunoglobulin specific for an antigen naturally or artificially placed upon the surface of an indicator particle coupled through the use of a hetero-bifunctional coupling reagent to a second antibody of differing specificity and specific for the antigen to be detected. In a preferred embodiment, a hetero-bifunctional coupling agent couples via a sulfhydryl group, a univalent immunoglobulin specific for the surface antigens on erythrocytes to a second multivalent immunoglobulin through an amide linkage with the latter immunoglobulin wherein said second immunoglobulin is specific for hepatitis-B surface antigen.Type: GrantFiled: September 8, 1981Date of Patent: February 21, 1984Assignee: Ortho Diagnostic Systems Inc.Inventors: Chi-Deu Chang, Henry A. Graham, Jr.
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Patent number: 4410634Abstract: The method comprises covalently binding an immuno-reactive to a selected macromolecular carrier and then contacting the resulting hapten-carrier conjugate at a selected concentration in a liquid phase with a selected solid phase until a desired amount of the hapten-carrier conjugate is adsorbed to the surface of the solid phase. Unbound hapten-carrier conjugate is then separated from the solid phase, and the solid phase containing the bound hapten-carrier conjugate is recovered for use in quantitative immunoassays and the like. The solid phase can be, for example, surfaces of a test tube or microtiter well or the like. The method is simple and inexpensive and permits hapten assays of improved sensitivity.Type: GrantFiled: November 12, 1981Date of Patent: October 18, 1983Assignee: Dynasciences CorporationInventors: Harold R. Cooper, Andrew O'Beirne
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Patent number: 4407965Abstract: A process for preparing a gut glucagon antigen composed of a peptide-carrier complex is disclosed, which comprises using as hapten a peptide represented by the following general formula;R-Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala-OHwherein R represents a hydrogen atom, an amino acid group or a peptide group containing 2 to 20 amino acid groups, and reacting it with a carrier in the presence of a binding agent for binding the hapten and the carrier to each other. Also, a process for preparing a gut glucagon antibody, which comprises administering the antigen described to mammals, and collecting the thus-produced antibody, is disclosed.Type: GrantFiled: May 21, 1981Date of Patent: October 4, 1983Assignee: Otsuka Pharmaceutical Co., Ltd.Inventor: Noboru Yanaihara
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Patent number: 4397959Abstract: Process for the preparation of test reagents comprising antigens or antibodies adsorbed on a surface, for example, the surface of synthetic or natural polymer particles in which the test material to be adsorbed is dissolved in a solvent in contact with the adsorbing surface and precipitated by the addition of a liquid which is miscible with the solvent, but does not dissolve the test material.Type: GrantFiled: November 5, 1980Date of Patent: August 9, 1983Assignee: Research CorporationInventor: Karim E. Hechemy
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Patent number: 4379780Abstract: A hapten is obtained by replacing the 3-hydroxy group of 17 .alpha.-dihydroequilin with HO--CO--A--O-- wherein A is an alkylene of one to six carbon atoms. The hapten is conjugated with an immunological carrier to provide an immunogen, which in turn produces a specific antiserum to 17 .alpha.-dihydroequilin. The antiserum is used in a radioimmunoassay for 17 .alpha.-dihydroequilin.Type: GrantFiled: September 29, 1980Date of Patent: April 12, 1983Assignee: American Home Products CorporationInventor: Pemmaraju N. Rao
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Patent number: 4379779Abstract: A hapten is obtained by replacing the 3-hydroxy group of equilin with HO--CO--A--O-- wherein A is an alkylene of one to six carbon atoms. The hapten is conjugated with an immunological carrier to provide an immunogen, which in turn produces a specific antiserum to equilin. The antiserum is used in a radioimmunoassay for equilin.Type: GrantFiled: September 29, 1980Date of Patent: April 12, 1983Assignee: American Home Products CorporationInventors: Pemmaraju N. Rao, Robert H. Purdy, Perry H. Moore, Jr.