Glycoproteins (e.g., Hormone, Etc.) Patents (Class 436/87)
  • Patent number: 12027356
    Abstract: Apparatus is provided and an IDA method is modified to detect and separately dissociate alkali-metal adducts of a compound. An ion source device ionizes one or more compounds of a sample, producing an ion beam. A mass filter selects a mass range of precursor ions from the ion beam, a mass analyzer measures intensities and m/z values of the precursor ions, and one or more of the precursor ions are selected for a peak list. For each pair of precursor ions of the peak list, if an m/z difference between the pair corresponds to an m/z difference between an alkali metal ion and another alkali metal ion or a proton, an ExD device is used to dissociate one precursor ion or both precursor ions of the pair using the processor. A CID device is used to dissociate all other precursor ions of the peak list.
    Type: Grant
    Filed: July 22, 2020
    Date of Patent: July 2, 2024
    Assignees: DH Technologies Development Pte. Ltd., University of Geneva
    Inventors: GĂ©rard Hopfgartner, Anita Orsolya Ducati, Ronald F. Bonner, Yves Le Blanc
  • Patent number: 11781127
    Abstract: The present invention provides a method for the isolation of sperm DNA from swabs taken from rape victims without having to perform a change in buffers. Non-sperm cells from the victim are digested with an enzyme and solubilized, and then in the same buffer an enzyme capable of digesting soluble DNA is added and the victim's DNA is degraded, leaving only the rapist's DNA intact. Since no change of buffer is needed, no centrifugation or filtration steps are needed. The inventive method has utility particularly in the forensic science field.
    Type: Grant
    Filed: January 11, 2008
    Date of Patent: October 10, 2023
    Inventor: Alex Garvin
  • Patent number: 11674969
    Abstract: Provided herein are methods for high throughput quantitation of testosterone utilizing at least two different derivatizing agents of different masses. In another aspect, provided herein are methods for determining the amount of testosterone in each of a plurality of human samples with a single mass spectrometric assay by subjecting each of a plurality of human samples to a different derivatizing agent to generate a differently derivatized testosterone in each of the plurality of samples; combining the plurality of samples to form a multiplex sample; and quantifying the amount of testosterone in each sample by mass spectrometry.
    Type: Grant
    Filed: March 15, 2019
    Date of Patent: June 13, 2023
    Assignee: Quest Diagnostics Investments LLC
    Inventors: Mildred M. Goldman, Scott Goldman, Julia Colletti, Nigel Clarke
  • Patent number: 11002715
    Abstract: In an LC/MS analysis of a sample containing various compounds, additive supply pumps 164A and 164B in a post-column adding unit 16 draw and supply different kinds of additives A and B from containers 163A and 163B, respectively. The additives are mixed into an eluate through T-joints 162 and 161. A preferable combination of the additives is the combination of DMSO which produces the effect of gathering charge states and 2-propanol which produces the effect of promoting atomization or vaporization of droplets. By mixing the two additives into the eluate while mixing them at an appropriate flow-rate ratio according to a previously determined flow-rate program, the ionization efficiency can be nearly optimized for each compound during the process of generating ions by spraying electrically charged droplets of the eluate from an ESI spray 21. Consequently, the detection sensitivity becomes higher than conventional levels.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: May 11, 2021
    Assignee: SHIMADZU CORPORATION
    Inventor: Yusaku Hioki
  • Patent number: 10371608
    Abstract: The invention discloses a cellulose or glass fiber paper for preservation of biological samples, which comprises 4-30 wt % of a hydrophilic branched carbohydrate polymer. It also discloses a method for preservation of biological samples by applying and drying them on the paper.
    Type: Grant
    Filed: October 29, 2012
    Date of Patent: August 6, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Mattias Algotsson, Miles W. Burrows, Jimmy Hedin Dahlstrom, Ylva Laurin, Ronnie Palmgren, Jinyu Zou
  • Patent number: 10371704
    Abstract: A method of analyzing diagnostic information, the method including: measuring a concentration of a mucin-1 (Tn-MUC1), which is reactive with a lectin that recognizes and binds to an N-acetyl-D-galactosamine?serine (threonine) residue, in a blood sample originated from a subject to be diagnosed; comparing the measured value with a threshold value; and estimating that a disease affecting the subject to be diagnosed is not prostate cancer but a benign prostate disease when the measured value of the concentration of Tn-MUC1 is greater than the threshold value.
    Type: Grant
    Filed: December 27, 2013
    Date of Patent: August 6, 2019
    Assignee: KONICA MINOLTA, INC.
    Inventors: Katsuko Yamashita, Takatoshi Kaya, Youichi Ide, Tomonori Kaneko
  • Patent number: 9885727
    Abstract: The present invention relates to methods of diagnosing, monitoring, and treating elastin fiber injuries. In additional preferred embodiments, the present invention relates to methods of validating candidate compounds for use in treating chronic obstructive pulmonary disease (COPD), chronic bronchitis, emphysema, refractory asthma, and other related diseases. Examples of such methods include determining if the candidate compound decreases the degradation of elastic fiber in a patient administered the candidate compound by measuring, using mass spectrometry employing an internal standard, a marker of elastic fiber degradation in a sample of a body fluid or a tissue of the patient. The invention provides that a decrease in the presence of the marker compared to a control validates that the candidate compound is effective to treat, prevent, or ameliorate the disease.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: February 6, 2018
    Assignees: St. Luke's-Roosevelt Hospital Center, The Trustees of Columbia University in the City of New York
    Inventors: Gerard M Turino, Shuren Ma, Yong Y Lin, Seymour Leiberman
  • Patent number: 9488653
    Abstract: A laboratory method and system used to isolate and analyze O-linked oligosaccharides from glycoproteins that uses non-reductive ?-elimination (NBRE). The method including the step of providing a predetermined amount of solution and then passing it through an ion exchange resin. The method further includes collecting a second solution off of the ion exchange resin and adding a predetermined amount of sodium hydroxide to form a third solution. The third solution is allowed to stand for a set amount of time at a particular temperature. The third solution is then washed through an ion exchange cartridge in the ammonium form. The collected fourth solution is evaporated and pushed through a sodium form resin prior to being analyzed for its composition.
    Type: Grant
    Filed: March 13, 2015
    Date of Patent: November 8, 2016
    Inventor: Michael A. Madson
  • Patent number: 9128085
    Abstract: This invention describes a rapid assay for measuring the ratio of glycated albumin to total albumin in blood. Patients with diabetes have elevated levels of glucose in their blood that can react with plasma albumin to form glycated albumin. The amount of glycated albumin formed is directly correlated with the level of plasma glucose that the albumin has been exposed to over a period of time. The ratio of glycated albumin to total albumin in blood will provide an indication of the average amount of protein glycation that occurred over the preceding 2-3 week period. The test is performed using a disposable strip or cassette that contains the testing reagents and the results are measured in a measuring instrument that automatically reads, calculates and displays the final result. The results of tests performed over a period of time are stored in the instrument's memory and presented in a numerical or graphical format so that the individual's glycated albumin level can be monitored over time.
    Type: Grant
    Filed: June 8, 2009
    Date of Patent: September 8, 2015
    Assignee: Epinex Diagnostics, Inc.
    Inventor: Henry John Smith
  • Patent number: 9057735
    Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more markers selected from the group consisting of cytochrome c and insulin-like growth factor IA as diagnostic and prognostic biomarkers in renal injuries.
    Type: Grant
    Filed: August 28, 2009
    Date of Patent: June 16, 2015
    Assignee: ASTUTE MEDICAL, INC.
    Inventors: Joseph Anderberg, Jeff Gray, Paul McPherson, Kevin Nakamura
  • Publication number: 20150118155
    Abstract: The present invention provides a method of diagnosing the existence or risk of hyperthyroidism in a feline comprising measuring the level of expression of one or more biomarkers selected from the group consisting of e.g., IYD, TG, SLC5A5, NIS, TPO, TSHR, DUOX1, DUOX2 (ThOX), TGFB1, CSTD, DCN and SEPP1 and the expression products thereof, in a biological sample from the feline, wherein elevated expression of the one or more biomarkers in the sample relative to a control value for expression in a sample from a normal feline or feline population, or a baseline value from the feline, indicates the existence or risk of hyperthyroidism; a method of treating a feline so diagnosed; and compositions, reagents and kits for carrying out the specified methods.
    Type: Application
    Filed: June 14, 2012
    Publication date: April 30, 2015
    Applicant: Hill's Pet Nutrition, Inc.
    Inventors: Samer Al-Murrani, Xiangming Gao
  • Publication number: 20150099669
    Abstract: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 9, 2015
    Inventors: Hui Zhang, Xingde Li, Shadi Toghi Eshghi
  • Publication number: 20150099306
    Abstract: A reading device for reading and analyzing a test cassette having an identification code includes a reading unit, used for reading the identification code marked by the test cassette and carrying out interpretation on a plurality of test lines of the test cassette; an operating unit, used for operating the reading device; a processing unit, used for interpreting a test result with the identification code and the test lines; and a display unit, used for displaying the test result of the test cassette.
    Type: Application
    Filed: April 7, 2014
    Publication date: April 9, 2015
    Inventor: HUI-CHI KU
  • Publication number: 20150093760
    Abstract: A cartridge for measuring a concentration of a glycated protein in a wide measurement range, a system for measuring a glycated protein, and a method of measuring a glycated protein using same.
    Type: Application
    Filed: October 1, 2014
    Publication date: April 2, 2015
    Inventors: Sang-kyu Kim, Kyung-mi Song, Jin-mi Oh, Youn-suk Choi
  • Publication number: 20150056190
    Abstract: The invention provides methods and compositions to detect expression of one or more biomarkers for identifying and treating patients who are likely to be responsive to VEGF antagonist therapy. The invention also provides kits and articles of manufacture for use in the methods.
    Type: Application
    Filed: March 14, 2013
    Publication date: February 26, 2015
    Applicant: Genentech, Inc.
    Inventors: Priti Hegde, Maike Schmidt, Ru-Fang Yeh
  • Patent number: 8962811
    Abstract: Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
    Type: Grant
    Filed: April 3, 2013
    Date of Patent: February 24, 2015
    Assignee: Ohio University
    Inventors: Marcia J. Kieliszewski, Jianfeng Xu, John Kopchick, Shigeru Okada, Gary Meyer
  • Patent number: 8940538
    Abstract: The present invention relates to an apparatus for quantifying the binding and dissociation kinetics of molecular interactions of small molecular bio materials with high sensitivity almost without the influence of a change in the reflective index resulting from a buffer solution by making polarized incident light incident on the binding layer of a bio material, formed in a thin dielectric film, so that the polarized incident light satisfies a p-wave non-reflecting condition and a quantifying method using the same.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: January 27, 2015
    Assignee: Korea Research Institute of Standards and Science
    Inventors: Hyun Mo Cho, Gal Won Che, Yong Jai Cho
  • Patent number: 8932864
    Abstract: Provided is a method and device for identifying glycated protein in a sample using a matrix including a boronic acid moiety that binds glycated proteins.
    Type: Grant
    Filed: March 18, 2013
    Date of Patent: January 13, 2015
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Sang-kyu Kim, Sung-ha Park, Kyung-mi Song, Soo-suk Lee, Youn-suk Choi
  • Publication number: 20150005192
    Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.
    Type: Application
    Filed: September 12, 2014
    Publication date: January 1, 2015
    Applicant: Cambridge Enterprise Limited
    Inventors: Sabine Bahn, Paul Guest, Laura Harris, Yishai Levin, Divya Krishnamurthy
  • Patent number: 8921773
    Abstract: Techniques are described for performing mass spectrometry. A stream of one or more ions is generated. The stream is transmitted into a collision cell over a period of time. In accordance with a set of criteria including a retention time of one or more precursor ions, a collision energy of the collision cell is selected to generate one or more product ions for said one or more precursor ions in said stream.
    Type: Grant
    Filed: January 19, 2011
    Date of Patent: December 30, 2014
    Assignee: Waters Technologies Corporation
    Inventors: Scott J. Geromanos, Johannes P. C. Vissers, Craig A. Dorschel, Asish B. Chakraborty, Welbin Chen, Jeffrey Cruz Silva
  • Publication number: 20140356968
    Abstract: The present disclosure provides methods to assess structural similarity of a first biomolecule and a second biomolecule by detecting one or more responses of the first and second biomolecule to thermodynamic stress conditions induced by osmotic and dielectric changes including, detecting a shift in fluorescence emission and/or a change in the intensity of the emission.
    Type: Application
    Filed: August 7, 2013
    Publication date: December 4, 2014
    Applicant: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz K. Niazi
  • Patent number: 8900877
    Abstract: A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: December 2, 2014
    Assignee: Luminex Corporation
    Inventors: Steve Jia Chang Yu, Jesus Ching, Phillip You Fai Lee, David Hsiang Hu
  • Publication number: 20140348748
    Abstract: Derivatives of benzothiazolylbenzeneamines useful as imaging agents for nuclear imaging such as positron emission tomography of beta-amyloids are described. Methods of detecting amyloid using the compounds are described. Also disclosed is a radiolabeling method for selected compounds.
    Type: Application
    Filed: September 13, 2012
    Publication date: November 27, 2014
    Inventors: Lisheng Cai, Victor W. Pike
  • Publication number: 20140323595
    Abstract: We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of microparticles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phosphotidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.
    Type: Application
    Filed: November 30, 2012
    Publication date: October 30, 2014
    Inventors: Sai Kiang Lim, Kok Hian Tan
  • Publication number: 20140323322
    Abstract: A chemical sensor comprising: a hydrogel layer, comprising one or more molecular recognition agents and a 2DPC self-assembling array; and a mirror layer. A method for analyzing a sample or bodily fluid, comprising: obtaining a sample or bodily fluid; placing an amount of the sample or bodily fluid onto a chemical sensor, comprising: a hydrogel layer, comprising a molecular recognition agent and a 2DPC self-assembling array; a tethering hydrogel layer; a mirror layer; and a membrane filter layer, allowing the bodily fluid to interact with the hydrogel layer; and allowing ambient or artificial light to pass through the hydrogel layer onto the mirror layer and observing a change in diffraction versus a control.
    Type: Application
    Filed: October 4, 2012
    Publication date: October 30, 2014
    Inventor: Sanford A. Asher
  • Patent number: 8871518
    Abstract: A monoclonal antibody that does not show a crossreactivity with middle-molecular weight (MMW) adiponectin and specifically reacts with high-molecular weight (HMW) adiponectin alone is disclosed. The monoclonal antibody of the present invention can be produced by using HMW adiponectin as an antigen. According to the monoclonal antibody of the present invention, a convenient, high-accurate, and versatile reagent for analyzing HMW adiponectin can be provided.
    Type: Grant
    Filed: May 6, 2010
    Date of Patent: October 28, 2014
    Assignees: Otsuka Pharmaceutical Co., Ltd., LSI Medience Corporation
    Inventors: Suguru Akamatsu, Kiyonori Katsuragi, Hideaki Onishi, Midori Abe, Toru Hadama, Ayako Nishimura, Mio Ooguchi
  • Publication number: 20140235473
    Abstract: The present Invention provides a method for diagnosing and/or prognosing Parkinson's disease dementia (PDD) comprising the step of detecting O-glycosylation. in a protein comprising a Ser/Thr motive, in particular Serpin A1, and/or the level of sialic acid on a protein comprising a Ser/Thr motive, in particular Serpin A1. Further, the present invention relates to a molecule for detecting O-linked glycomoieties in a protein comprising a Ser/Thr motive, in particular Serpin A1, and/or glycosylated i so forms of a Ser/Thr motive comprising protein, in particular Serpin A1, for use in the diagnosis and/or prognosis of Parkinson's disease dementia (PDD), Furthermore, the present invention relates to means for diagnosing and/or prognosing Parkinson's disease dementia (PDD) and a kit for diagnosing and/or prognosing Parkinson's disease dementia (PDD).
    Type: Application
    Filed: June 22, 2012
    Publication date: August 21, 2014
    Inventors: Markus Otto, Stefan Lehnert, Sarah Jesse, Olaf Jahn
  • Patent number: 8809062
    Abstract: A method (100) for analyzing chemicals includes fractionating a complex sample into at least two sample portions that each includes potions of two polypeptides though in different concentration ratios, digesting and performing LC/MS on each of the sample portions (110, and associating precursor ions observed via LC/MS with their corresponding polypeptide in response to LC/MS provided intensity data (170). A set of precursor ions that has substantially similar intensity ratios in both sample portions is determined to be associated with the same polypeptide.
    Type: Grant
    Filed: April 16, 2013
    Date of Patent: August 19, 2014
    Assignee: Waters Technologies Corporation
    Inventors: Marc Victor Gorenstein, Scott J. Geromanos, Jeffrey Cruz Silva, Guo-Zhong Li
  • Publication number: 20140213459
    Abstract: The present invention relates to methods for improving the folding stability of antibodies, to antibodies with improved folding stability, nucleic acid and vectors encoding such antibodies, and to uses of such antibodies, nucleic acid and vectors.
    Type: Application
    Filed: May 29, 2012
    Publication date: July 31, 2014
    Inventor: Roland Beckmann
  • Patent number: 8778699
    Abstract: A method for determining the amount of NT-proBNP in blood samples from animals. The method includes detecting degradation products of NT-proBNP by various methods, including using antibodies, kits and device.
    Type: Grant
    Filed: August 4, 2011
    Date of Patent: July 15, 2014
    Assignee: Idexx Laboratories, Inc.
    Inventors: Mahalakshmi Yerramilli, Michael Atkinson, Murthy V. S. N. Yerramilli
  • Patent number: 8741662
    Abstract: Methods and kits for diagnosis and prognosis using biomarkers comprising albumin-bound protein/peptide complex (ABPPC).
    Type: Grant
    Filed: June 14, 2007
    Date of Patent: June 3, 2014
    Assignee: The Johns Hopkin University
    Inventors: Jennifer E. Van Eyk, Rebekah Lynn Gundry, Robert J. Cotter
  • Publication number: 20140127735
    Abstract: The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.
    Type: Application
    Filed: January 14, 2014
    Publication date: May 8, 2014
    Applicant: Momenta Pharmaceuticals, Inc.
    Inventors: Ian Christopher Parsons, Jonathan Lansing, Daniela Becatti, Scott Bailey, Carlos J. Bosques
  • Patent number: 8716472
    Abstract: A tri-podal compound according to formula (I) wherein, each Z is the same and is a substituted or unsubstituted N-heteroaromatic single-, multiple-, or fused-ring; and each A is the same, and can represent a direct bond between the cyclohexane ring and Z, or a carboxamide group (—C(O)—N(H)—). Use of the compounds in combinatorial libraries, methods of making the tripodal compounds, sensor devices for detecting carbohydrate targets, and methods of using the tripodal compounds to detect carbohydrate targets in a sample are also disclosed.
    Type: Grant
    Filed: October 16, 2007
    Date of Patent: May 6, 2014
    Assignee: University of Rochester
    Inventors: Benjamin L. Miller, Prakash B. Palde, Peter C. Gareiss
  • Patent number: 8703498
    Abstract: A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: April 22, 2014
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventors: Ian Christopher Parsons, Ting Zheng, Nur Sibel Gunay, Carlos J. Bosques
  • Publication number: 20140107181
    Abstract: The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inducing anabolic stimulation of chondrocytes, including cartilage formation enhancing pharmaceutical compositions, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean cartilage thickness in a subject.
    Type: Application
    Filed: October 17, 2013
    Publication date: April 17, 2014
    Applicant: GALAPAGOS N.V.
    Inventors: Nick Vandeghinste, Peter Herwig Maria Tomme, Frits Michiels, Libin Ma, Blandine Mille-Baker, Helmuth H.G. van Es
  • Publication number: 20140099725
    Abstract: A method for the analysis of samples including one or more glycopeptides including the steps of separating one or more glycopeptides using a chromatography system to produce a chromatographic eluent, adding a supercharging reagent to the chromatographic eluent, providing the chromatographic eluent and supercharging reagent to a mass spectrometer, ionizing said chromatographic eluent and supercharging reagent in an ion source to produce glycopeptide ions, performing at least one ion ion reaction on at least some of the glycopeptide ions to produce fragment ions, mass analyzing the fragment ions to produce ion ion reaction mass spectral data, and interpreting the ion ion reaction data mass spectral data to provide structural information relating to the glycopeptide.
    Type: Application
    Filed: October 9, 2013
    Publication date: April 10, 2014
    Applicant: MICROMASS UK LIMITED
    Inventors: Jonathan P. Williams, Jeffery Mark Brown
  • Patent number: 8691585
    Abstract: The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.
    Type: Grant
    Filed: June 1, 2011
    Date of Patent: April 8, 2014
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: John F. O'Connor, Galina I. Kovalevskaya, Steven Birken
  • Publication number: 20140093493
    Abstract: The invention provides a method for reducing aggregation and inhibiting flocculation of a macromolecule, such as a protein, under physiological conditions, by the addition of certain cyclodextrins (CDs). The invention also provides a method to minimize inflammation at the injection site during subcutaneous administration of a macromolecule and pharmaceutical formulations for such administration. Further the invention provides methods of treating a CD20 positive cancer or an autoimmune disease, comprising administering a humanized anti-CD20 antibody in a pharmaceutical formulation of the invention. The invention further provides an in vitro dialysis method to evaluate the ability of an excipient to reduce aggregation of an antibody or other macromolecule under physiological conditions.
    Type: Application
    Filed: June 10, 2013
    Publication date: April 3, 2014
    Inventors: Brian Lobo, Sabrina Lo, Yuchang John Wang, Rita L. Wong
  • Patent number: 8673644
    Abstract: A method for identifying persons with increased risk of developing type 2 diabetes mellitus utilizing selected biomarkers described hereafter either alone or in combination. The present invention allows for broad based, reliable, screening of large population bases and provides other advantages, including the formulation of effective strategies for characterizing, archiving, and contrasting data from multiple sample types under varying conditions.
    Type: Grant
    Filed: May 13, 2008
    Date of Patent: March 18, 2014
    Assignee: Battelle Memorial Institute
    Inventors: Thomas O. Metz, Wei-Jun Qian, Jon M. Jacobs, Ashoka D. Polpitiya, David G. Camp, II, Richard D. Smith
  • Publication number: 20140072981
    Abstract: A cysteine hydrazide nicotinamide (Cyhn) reagent designed for the enrichment of bacterial glycoproteins is provided. Methods for purification of free oligosaccharides and their analysis are also provided.
    Type: Application
    Filed: September 4, 2013
    Publication date: March 13, 2014
    Applicant: California Institute of Technology
    Inventors: William M. Clemons, Kyoung-Soon Jang, Roger Nani, Sergiy Levin, Sarah Reisman
  • Patent number: 8669113
    Abstract: The present invention relates to methods, monitors and systems measuring lysophosphatidylcholine, its derivatives and/or procalcitonin as well as at least one clinical marker (e.g. temperature or respiration rate) and/or at least one biomarker for the early detection of sepsis in a subject.
    Type: Grant
    Filed: April 2, 2009
    Date of Patent: March 11, 2014
    Assignee: Becton, Dickinson and Company
    Inventors: Song Shi, Richard L. Moore, James Garrett
  • Publication number: 20140065714
    Abstract: Provided is a method and device for identifying glycated protein in a sample using a matrix including a boronic acid moiety that binds glycated proteins.
    Type: Application
    Filed: March 18, 2013
    Publication date: March 6, 2014
    Applicant: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Sang-kyu KIM, Sung-ha PARK, Kyung-mi SONG, Soo-suk LEE, Youn-suk CHOI
  • Publication number: 20140065716
    Abstract: A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.
    Type: Application
    Filed: November 8, 2013
    Publication date: March 6, 2014
    Applicant: MOMENTA PHARMACEUTICALS, INC.
    Inventors: Ian Christopher Parsons, Ting Zheng, Nur Sibel Gunay, Carlos J. Bosques
  • Publication number: 20140024124
    Abstract: A glycoprotein and/or a glycopeptide which are a test substance is heated in the presence of a pyrazolone derivative, an isoxazolone derivative, a hydantoin derivative, a rhodanine derivative, a maleimide derivative, or the like under a basic condition to cleave and label a post-translational modification group for analysis, thereby enabling analysis of a post-translational modification of a serine residue and/or a threonine residue.
    Type: Application
    Filed: February 16, 2012
    Publication date: January 23, 2014
    Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, CELLSEED INC.
    Inventors: Yasuro Shinohara, Yasuhiro Takegawa, Naoki Fujitani, Jun-ichi Furukawa, Hideaki Sakai
  • Patent number: 8628977
    Abstract: Reagents and methods are provided that permit simultaneous analysis of multiple diverse small molecule analytes present in a complex mixture. Samples are labeled with chemically identical but isotopically distinct forms of the labeling reagent, and analyzed using mass spectrometry. A single reagent simultaneously derivatizes multiple small molecule analytes having different reactive functional groups.
    Type: Grant
    Filed: May 1, 2009
    Date of Patent: January 14, 2014
    Assignee: Purdue Research Foundation
    Inventors: Jiri Adamec, Wen-Chu Yang, Fred E. Regnier
  • Patent number: 8623654
    Abstract: An object of the present invention is to provide a control material which can be preferably employed in detection of 1,5-anhydro-D-glucitol and glycoalbumin, which are employed as excellent indices for diabetes. The present inventor has found that mannitol which is added to serum or plasma used as control material stabilizes 1,5-anhydro-D-glucitol and glycoalbumin present in the serum or plasma for a long period of time, and that the object can be attained through provision of (1) an agent for stabilizing control material, the agent being composed of mannitol and (2) control material containing mannitol and 1,5-anhydro-D-glucitol.
    Type: Grant
    Filed: March 4, 2010
    Date of Patent: January 7, 2014
    Assignee: BML, Inc.
    Inventor: Isami Tsuboi
  • Publication number: 20130344524
    Abstract: In a method for detecting a glucagon-secretin family peptide in a sample, the sample is contacted with a cleaving agent capable of digesting the glucagon-secretin family peptide by cleaving a peptide bond of at least one aspartic acid within the glucagon-secretin family peptide and thereby generating a plurality of peptide fragments, at least one of the peptide fragments containing an N-terminal end of the glucagon-secretin family peptide. The peptide fragment that contains the N-terminal end of the glucagon-secretin family peptide is then detected using liquid chromatography and mass spectrometry. The amount of peptide fragment that contains the N-terminal end of the glucagon-secretin family peptide in the sample can then be quantitated using a calibration curve.
    Type: Application
    Filed: March 7, 2012
    Publication date: December 26, 2013
    Applicant: SANWA KAGAKU KENKYUSHO CO., LTD.
    Inventors: Atsushi Miyachi, Yoshika Yasuda, Hiroko Hirooka
  • Publication number: 20130344511
    Abstract: The invention provides, in certain embodiments, a method of detecting an indicator of renal injury or renal disease. The method entails assaying a urine sample for hematopoietic growth factor inducible neurokinin-1 (HGFIN), wherein the presence of HGFIN at an elevated level indicates the presence and/or degree of renal injury or renal disease, and/or the rate of loss of renal function. In other embodiments, the invention provides a method of detecting an indicator of systemic inflammation. This method entails assaying a biological sample for HGFIN, wherein the presence of HGFIN at an elevated level indicates the presence and/or degree of systemic inflammation. Also provided, are methods of determining progression of these conditions, as well as methods of determining subjects' response to treatment.
    Type: Application
    Filed: June 10, 2013
    Publication date: December 26, 2013
    Inventors: Sharon ADLER, Tiane DAI, Ying WANG, Mukti PATEL-CHAMBERLIN, Cynthia C. NAST, Janine LaPAGE, Nosratolah Dabir VAZIRI, Madeleine V. PAHL
  • Publication number: 20130337536
    Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
    Type: Application
    Filed: August 21, 2013
    Publication date: December 19, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Evan Antoulinakis, Kyle Gee, Aleksey Rukavishnikov
  • Patent number: RE45074
    Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: August 12, 2014
    Assignee: ARKRAY, Inc.
    Inventors: Satoshi Yonehara, Tsuguki Komori