Glycoproteins (e.g., Hormone, Etc.) Patents (Class 436/87)
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Patent number: 12027356Abstract: Apparatus is provided and an IDA method is modified to detect and separately dissociate alkali-metal adducts of a compound. An ion source device ionizes one or more compounds of a sample, producing an ion beam. A mass filter selects a mass range of precursor ions from the ion beam, a mass analyzer measures intensities and m/z values of the precursor ions, and one or more of the precursor ions are selected for a peak list. For each pair of precursor ions of the peak list, if an m/z difference between the pair corresponds to an m/z difference between an alkali metal ion and another alkali metal ion or a proton, an ExD device is used to dissociate one precursor ion or both precursor ions of the pair using the processor. A CID device is used to dissociate all other precursor ions of the peak list.Type: GrantFiled: July 22, 2020Date of Patent: July 2, 2024Assignees: DH Technologies Development Pte. Ltd., University of GenevaInventors: GĂ©rard Hopfgartner, Anita Orsolya Ducati, Ronald F. Bonner, Yves Le Blanc
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Patent number: 11781127Abstract: The present invention provides a method for the isolation of sperm DNA from swabs taken from rape victims without having to perform a change in buffers. Non-sperm cells from the victim are digested with an enzyme and solubilized, and then in the same buffer an enzyme capable of digesting soluble DNA is added and the victim's DNA is degraded, leaving only the rapist's DNA intact. Since no change of buffer is needed, no centrifugation or filtration steps are needed. The inventive method has utility particularly in the forensic science field.Type: GrantFiled: January 11, 2008Date of Patent: October 10, 2023Inventor: Alex Garvin
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Patent number: 11674969Abstract: Provided herein are methods for high throughput quantitation of testosterone utilizing at least two different derivatizing agents of different masses. In another aspect, provided herein are methods for determining the amount of testosterone in each of a plurality of human samples with a single mass spectrometric assay by subjecting each of a plurality of human samples to a different derivatizing agent to generate a differently derivatized testosterone in each of the plurality of samples; combining the plurality of samples to form a multiplex sample; and quantifying the amount of testosterone in each sample by mass spectrometry.Type: GrantFiled: March 15, 2019Date of Patent: June 13, 2023Assignee: Quest Diagnostics Investments LLCInventors: Mildred M. Goldman, Scott Goldman, Julia Colletti, Nigel Clarke
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Patent number: 11002715Abstract: In an LC/MS analysis of a sample containing various compounds, additive supply pumps 164A and 164B in a post-column adding unit 16 draw and supply different kinds of additives A and B from containers 163A and 163B, respectively. The additives are mixed into an eluate through T-joints 162 and 161. A preferable combination of the additives is the combination of DMSO which produces the effect of gathering charge states and 2-propanol which produces the effect of promoting atomization or vaporization of droplets. By mixing the two additives into the eluate while mixing them at an appropriate flow-rate ratio according to a previously determined flow-rate program, the ionization efficiency can be nearly optimized for each compound during the process of generating ions by spraying electrically charged droplets of the eluate from an ESI spray 21. Consequently, the detection sensitivity becomes higher than conventional levels.Type: GrantFiled: May 8, 2018Date of Patent: May 11, 2021Assignee: SHIMADZU CORPORATIONInventor: Yusaku Hioki
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Patent number: 10371608Abstract: The invention discloses a cellulose or glass fiber paper for preservation of biological samples, which comprises 4-30 wt % of a hydrophilic branched carbohydrate polymer. It also discloses a method for preservation of biological samples by applying and drying them on the paper.Type: GrantFiled: October 29, 2012Date of Patent: August 6, 2019Assignee: GE Healthcare Bio-Sciences ABInventors: Mattias Algotsson, Miles W. Burrows, Jimmy Hedin Dahlstrom, Ylva Laurin, Ronnie Palmgren, Jinyu Zou
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Patent number: 10371704Abstract: A method of analyzing diagnostic information, the method including: measuring a concentration of a mucin-1 (Tn-MUC1), which is reactive with a lectin that recognizes and binds to an N-acetyl-D-galactosamine?serine (threonine) residue, in a blood sample originated from a subject to be diagnosed; comparing the measured value with a threshold value; and estimating that a disease affecting the subject to be diagnosed is not prostate cancer but a benign prostate disease when the measured value of the concentration of Tn-MUC1 is greater than the threshold value.Type: GrantFiled: December 27, 2013Date of Patent: August 6, 2019Assignee: KONICA MINOLTA, INC.Inventors: Katsuko Yamashita, Takatoshi Kaya, Youichi Ide, Tomonori Kaneko
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Patent number: 9885727Abstract: The present invention relates to methods of diagnosing, monitoring, and treating elastin fiber injuries. In additional preferred embodiments, the present invention relates to methods of validating candidate compounds for use in treating chronic obstructive pulmonary disease (COPD), chronic bronchitis, emphysema, refractory asthma, and other related diseases. Examples of such methods include determining if the candidate compound decreases the degradation of elastic fiber in a patient administered the candidate compound by measuring, using mass spectrometry employing an internal standard, a marker of elastic fiber degradation in a sample of a body fluid or a tissue of the patient. The invention provides that a decrease in the presence of the marker compared to a control validates that the candidate compound is effective to treat, prevent, or ameliorate the disease.Type: GrantFiled: May 14, 2015Date of Patent: February 6, 2018Assignees: St. Luke's-Roosevelt Hospital Center, The Trustees of Columbia University in the City of New YorkInventors: Gerard M Turino, Shuren Ma, Yong Y Lin, Seymour Leiberman
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Patent number: 9488653Abstract: A laboratory method and system used to isolate and analyze O-linked oligosaccharides from glycoproteins that uses non-reductive ?-elimination (NBRE). The method including the step of providing a predetermined amount of solution and then passing it through an ion exchange resin. The method further includes collecting a second solution off of the ion exchange resin and adding a predetermined amount of sodium hydroxide to form a third solution. The third solution is allowed to stand for a set amount of time at a particular temperature. The third solution is then washed through an ion exchange cartridge in the ammonium form. The collected fourth solution is evaporated and pushed through a sodium form resin prior to being analyzed for its composition.Type: GrantFiled: March 13, 2015Date of Patent: November 8, 2016Inventor: Michael A. Madson
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Patent number: 9128085Abstract: This invention describes a rapid assay for measuring the ratio of glycated albumin to total albumin in blood. Patients with diabetes have elevated levels of glucose in their blood that can react with plasma albumin to form glycated albumin. The amount of glycated albumin formed is directly correlated with the level of plasma glucose that the albumin has been exposed to over a period of time. The ratio of glycated albumin to total albumin in blood will provide an indication of the average amount of protein glycation that occurred over the preceding 2-3 week period. The test is performed using a disposable strip or cassette that contains the testing reagents and the results are measured in a measuring instrument that automatically reads, calculates and displays the final result. The results of tests performed over a period of time are stored in the instrument's memory and presented in a numerical or graphical format so that the individual's glycated albumin level can be monitored over time.Type: GrantFiled: June 8, 2009Date of Patent: September 8, 2015Assignee: Epinex Diagnostics, Inc.Inventor: Henry John Smith
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Patent number: 9057735Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more markers selected from the group consisting of cytochrome c and insulin-like growth factor IA as diagnostic and prognostic biomarkers in renal injuries.Type: GrantFiled: August 28, 2009Date of Patent: June 16, 2015Assignee: ASTUTE MEDICAL, INC.Inventors: Joseph Anderberg, Jeff Gray, Paul McPherson, Kevin Nakamura
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Publication number: 20150118155Abstract: The present invention provides a method of diagnosing the existence or risk of hyperthyroidism in a feline comprising measuring the level of expression of one or more biomarkers selected from the group consisting of e.g., IYD, TG, SLC5A5, NIS, TPO, TSHR, DUOX1, DUOX2 (ThOX), TGFB1, CSTD, DCN and SEPP1 and the expression products thereof, in a biological sample from the feline, wherein elevated expression of the one or more biomarkers in the sample relative to a control value for expression in a sample from a normal feline or feline population, or a baseline value from the feline, indicates the existence or risk of hyperthyroidism; a method of treating a feline so diagnosed; and compositions, reagents and kits for carrying out the specified methods.Type: ApplicationFiled: June 14, 2012Publication date: April 30, 2015Applicant: Hill's Pet Nutrition, Inc.Inventors: Samer Al-Murrani, Xiangming Gao
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Publication number: 20150099669Abstract: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement.Type: ApplicationFiled: May 23, 2013Publication date: April 9, 2015Inventors: Hui Zhang, Xingde Li, Shadi Toghi Eshghi
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Publication number: 20150099306Abstract: A reading device for reading and analyzing a test cassette having an identification code includes a reading unit, used for reading the identification code marked by the test cassette and carrying out interpretation on a plurality of test lines of the test cassette; an operating unit, used for operating the reading device; a processing unit, used for interpreting a test result with the identification code and the test lines; and a display unit, used for displaying the test result of the test cassette.Type: ApplicationFiled: April 7, 2014Publication date: April 9, 2015Inventor: HUI-CHI KU
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Publication number: 20150093760Abstract: A cartridge for measuring a concentration of a glycated protein in a wide measurement range, a system for measuring a glycated protein, and a method of measuring a glycated protein using same.Type: ApplicationFiled: October 1, 2014Publication date: April 2, 2015Inventors: Sang-kyu Kim, Kyung-mi Song, Jin-mi Oh, Youn-suk Choi
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Publication number: 20150056190Abstract: The invention provides methods and compositions to detect expression of one or more biomarkers for identifying and treating patients who are likely to be responsive to VEGF antagonist therapy. The invention also provides kits and articles of manufacture for use in the methods.Type: ApplicationFiled: March 14, 2013Publication date: February 26, 2015Applicant: Genentech, Inc.Inventors: Priti Hegde, Maike Schmidt, Ru-Fang Yeh
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Patent number: 8962811Abstract: Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.Type: GrantFiled: April 3, 2013Date of Patent: February 24, 2015Assignee: Ohio UniversityInventors: Marcia J. Kieliszewski, Jianfeng Xu, John Kopchick, Shigeru Okada, Gary Meyer
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Patent number: 8940538Abstract: The present invention relates to an apparatus for quantifying the binding and dissociation kinetics of molecular interactions of small molecular bio materials with high sensitivity almost without the influence of a change in the reflective index resulting from a buffer solution by making polarized incident light incident on the binding layer of a bio material, formed in a thin dielectric film, so that the polarized incident light satisfies a p-wave non-reflecting condition and a quantifying method using the same.Type: GrantFiled: October 29, 2010Date of Patent: January 27, 2015Assignee: Korea Research Institute of Standards and ScienceInventors: Hyun Mo Cho, Gal Won Che, Yong Jai Cho
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Patent number: 8932864Abstract: Provided is a method and device for identifying glycated protein in a sample using a matrix including a boronic acid moiety that binds glycated proteins.Type: GrantFiled: March 18, 2013Date of Patent: January 13, 2015Assignee: Samsung Electronics Co., Ltd.Inventors: Sang-kyu Kim, Sung-ha Park, Kyung-mi Song, Soo-suk Lee, Youn-suk Choi
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Publication number: 20150005192Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.Type: ApplicationFiled: September 12, 2014Publication date: January 1, 2015Applicant: Cambridge Enterprise LimitedInventors: Sabine Bahn, Paul Guest, Laura Harris, Yishai Levin, Divya Krishnamurthy
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Patent number: 8921773Abstract: Techniques are described for performing mass spectrometry. A stream of one or more ions is generated. The stream is transmitted into a collision cell over a period of time. In accordance with a set of criteria including a retention time of one or more precursor ions, a collision energy of the collision cell is selected to generate one or more product ions for said one or more precursor ions in said stream.Type: GrantFiled: January 19, 2011Date of Patent: December 30, 2014Assignee: Waters Technologies CorporationInventors: Scott J. Geromanos, Johannes P. C. Vissers, Craig A. Dorschel, Asish B. Chakraborty, Welbin Chen, Jeffrey Cruz Silva
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Publication number: 20140356968Abstract: The present disclosure provides methods to assess structural similarity of a first biomolecule and a second biomolecule by detecting one or more responses of the first and second biomolecule to thermodynamic stress conditions induced by osmotic and dielectric changes including, detecting a shift in fluorescence emission and/or a change in the intensity of the emission.Type: ApplicationFiled: August 7, 2013Publication date: December 4, 2014Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 8900877Abstract: A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.Type: GrantFiled: April 30, 2012Date of Patent: December 2, 2014Assignee: Luminex CorporationInventors: Steve Jia Chang Yu, Jesus Ching, Phillip You Fai Lee, David Hsiang Hu
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Publication number: 20140348748Abstract: Derivatives of benzothiazolylbenzeneamines useful as imaging agents for nuclear imaging such as positron emission tomography of beta-amyloids are described. Methods of detecting amyloid using the compounds are described. Also disclosed is a radiolabeling method for selected compounds.Type: ApplicationFiled: September 13, 2012Publication date: November 27, 2014Inventors: Lisheng Cai, Victor W. Pike
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Publication number: 20140323595Abstract: We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of microparticles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phosphotidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.Type: ApplicationFiled: November 30, 2012Publication date: October 30, 2014Inventors: Sai Kiang Lim, Kok Hian Tan
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Publication number: 20140323322Abstract: A chemical sensor comprising: a hydrogel layer, comprising one or more molecular recognition agents and a 2DPC self-assembling array; and a mirror layer. A method for analyzing a sample or bodily fluid, comprising: obtaining a sample or bodily fluid; placing an amount of the sample or bodily fluid onto a chemical sensor, comprising: a hydrogel layer, comprising a molecular recognition agent and a 2DPC self-assembling array; a tethering hydrogel layer; a mirror layer; and a membrane filter layer, allowing the bodily fluid to interact with the hydrogel layer; and allowing ambient or artificial light to pass through the hydrogel layer onto the mirror layer and observing a change in diffraction versus a control.Type: ApplicationFiled: October 4, 2012Publication date: October 30, 2014Inventor: Sanford A. Asher
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Patent number: 8871518Abstract: A monoclonal antibody that does not show a crossreactivity with middle-molecular weight (MMW) adiponectin and specifically reacts with high-molecular weight (HMW) adiponectin alone is disclosed. The monoclonal antibody of the present invention can be produced by using HMW adiponectin as an antigen. According to the monoclonal antibody of the present invention, a convenient, high-accurate, and versatile reagent for analyzing HMW adiponectin can be provided.Type: GrantFiled: May 6, 2010Date of Patent: October 28, 2014Assignees: Otsuka Pharmaceutical Co., Ltd., LSI Medience CorporationInventors: Suguru Akamatsu, Kiyonori Katsuragi, Hideaki Onishi, Midori Abe, Toru Hadama, Ayako Nishimura, Mio Ooguchi
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Publication number: 20140235473Abstract: The present Invention provides a method for diagnosing and/or prognosing Parkinson's disease dementia (PDD) comprising the step of detecting O-glycosylation. in a protein comprising a Ser/Thr motive, in particular Serpin A1, and/or the level of sialic acid on a protein comprising a Ser/Thr motive, in particular Serpin A1. Further, the present invention relates to a molecule for detecting O-linked glycomoieties in a protein comprising a Ser/Thr motive, in particular Serpin A1, and/or glycosylated i so forms of a Ser/Thr motive comprising protein, in particular Serpin A1, for use in the diagnosis and/or prognosis of Parkinson's disease dementia (PDD), Furthermore, the present invention relates to means for diagnosing and/or prognosing Parkinson's disease dementia (PDD) and a kit for diagnosing and/or prognosing Parkinson's disease dementia (PDD).Type: ApplicationFiled: June 22, 2012Publication date: August 21, 2014Inventors: Markus Otto, Stefan Lehnert, Sarah Jesse, Olaf Jahn
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Patent number: 8809062Abstract: A method (100) for analyzing chemicals includes fractionating a complex sample into at least two sample portions that each includes potions of two polypeptides though in different concentration ratios, digesting and performing LC/MS on each of the sample portions (110, and associating precursor ions observed via LC/MS with their corresponding polypeptide in response to LC/MS provided intensity data (170). A set of precursor ions that has substantially similar intensity ratios in both sample portions is determined to be associated with the same polypeptide.Type: GrantFiled: April 16, 2013Date of Patent: August 19, 2014Assignee: Waters Technologies CorporationInventors: Marc Victor Gorenstein, Scott J. Geromanos, Jeffrey Cruz Silva, Guo-Zhong Li
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Publication number: 20140213459Abstract: The present invention relates to methods for improving the folding stability of antibodies, to antibodies with improved folding stability, nucleic acid and vectors encoding such antibodies, and to uses of such antibodies, nucleic acid and vectors.Type: ApplicationFiled: May 29, 2012Publication date: July 31, 2014Inventor: Roland Beckmann
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Patent number: 8778699Abstract: A method for determining the amount of NT-proBNP in blood samples from animals. The method includes detecting degradation products of NT-proBNP by various methods, including using antibodies, kits and device.Type: GrantFiled: August 4, 2011Date of Patent: July 15, 2014Assignee: Idexx Laboratories, Inc.Inventors: Mahalakshmi Yerramilli, Michael Atkinson, Murthy V. S. N. Yerramilli
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Patent number: 8741662Abstract: Methods and kits for diagnosis and prognosis using biomarkers comprising albumin-bound protein/peptide complex (ABPPC).Type: GrantFiled: June 14, 2007Date of Patent: June 3, 2014Assignee: The Johns Hopkin UniversityInventors: Jennifer E. Van Eyk, Rebekah Lynn Gundry, Robert J. Cotter
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Publication number: 20140127735Abstract: The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.Type: ApplicationFiled: January 14, 2014Publication date: May 8, 2014Applicant: Momenta Pharmaceuticals, Inc.Inventors: Ian Christopher Parsons, Jonathan Lansing, Daniela Becatti, Scott Bailey, Carlos J. Bosques
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Patent number: 8716472Abstract: A tri-podal compound according to formula (I) wherein, each Z is the same and is a substituted or unsubstituted N-heteroaromatic single-, multiple-, or fused-ring; and each A is the same, and can represent a direct bond between the cyclohexane ring and Z, or a carboxamide group (—C(O)—N(H)—). Use of the compounds in combinatorial libraries, methods of making the tripodal compounds, sensor devices for detecting carbohydrate targets, and methods of using the tripodal compounds to detect carbohydrate targets in a sample are also disclosed.Type: GrantFiled: October 16, 2007Date of Patent: May 6, 2014Assignee: University of RochesterInventors: Benjamin L. Miller, Prakash B. Palde, Peter C. Gareiss
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Patent number: 8703498Abstract: A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.Type: GrantFiled: September 28, 2012Date of Patent: April 22, 2014Assignee: Momenta Pharmaceuticals, Inc.Inventors: Ian Christopher Parsons, Ting Zheng, Nur Sibel Gunay, Carlos J. Bosques
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Publication number: 20140107181Abstract: The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inducing anabolic stimulation of chondrocytes, including cartilage formation enhancing pharmaceutical compositions, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean cartilage thickness in a subject.Type: ApplicationFiled: October 17, 2013Publication date: April 17, 2014Applicant: GALAPAGOS N.V.Inventors: Nick Vandeghinste, Peter Herwig Maria Tomme, Frits Michiels, Libin Ma, Blandine Mille-Baker, Helmuth H.G. van Es
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Publication number: 20140099725Abstract: A method for the analysis of samples including one or more glycopeptides including the steps of separating one or more glycopeptides using a chromatography system to produce a chromatographic eluent, adding a supercharging reagent to the chromatographic eluent, providing the chromatographic eluent and supercharging reagent to a mass spectrometer, ionizing said chromatographic eluent and supercharging reagent in an ion source to produce glycopeptide ions, performing at least one ion ion reaction on at least some of the glycopeptide ions to produce fragment ions, mass analyzing the fragment ions to produce ion ion reaction mass spectral data, and interpreting the ion ion reaction data mass spectral data to provide structural information relating to the glycopeptide.Type: ApplicationFiled: October 9, 2013Publication date: April 10, 2014Applicant: MICROMASS UK LIMITEDInventors: Jonathan P. Williams, Jeffery Mark Brown
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Patent number: 8691585Abstract: The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.Type: GrantFiled: June 1, 2011Date of Patent: April 8, 2014Assignee: The Trustees of Columbia University in the City of New YorkInventors: John F. O'Connor, Galina I. Kovalevskaya, Steven Birken
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Publication number: 20140093493Abstract: The invention provides a method for reducing aggregation and inhibiting flocculation of a macromolecule, such as a protein, under physiological conditions, by the addition of certain cyclodextrins (CDs). The invention also provides a method to minimize inflammation at the injection site during subcutaneous administration of a macromolecule and pharmaceutical formulations for such administration. Further the invention provides methods of treating a CD20 positive cancer or an autoimmune disease, comprising administering a humanized anti-CD20 antibody in a pharmaceutical formulation of the invention. The invention further provides an in vitro dialysis method to evaluate the ability of an excipient to reduce aggregation of an antibody or other macromolecule under physiological conditions.Type: ApplicationFiled: June 10, 2013Publication date: April 3, 2014Inventors: Brian Lobo, Sabrina Lo, Yuchang John Wang, Rita L. Wong
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Patent number: 8673644Abstract: A method for identifying persons with increased risk of developing type 2 diabetes mellitus utilizing selected biomarkers described hereafter either alone or in combination. The present invention allows for broad based, reliable, screening of large population bases and provides other advantages, including the formulation of effective strategies for characterizing, archiving, and contrasting data from multiple sample types under varying conditions.Type: GrantFiled: May 13, 2008Date of Patent: March 18, 2014Assignee: Battelle Memorial InstituteInventors: Thomas O. Metz, Wei-Jun Qian, Jon M. Jacobs, Ashoka D. Polpitiya, David G. Camp, II, Richard D. Smith
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Publication number: 20140072981Abstract: A cysteine hydrazide nicotinamide (Cyhn) reagent designed for the enrichment of bacterial glycoproteins is provided. Methods for purification of free oligosaccharides and their analysis are also provided.Type: ApplicationFiled: September 4, 2013Publication date: March 13, 2014Applicant: California Institute of TechnologyInventors: William M. Clemons, Kyoung-Soon Jang, Roger Nani, Sergiy Levin, Sarah Reisman
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Patent number: 8669113Abstract: The present invention relates to methods, monitors and systems measuring lysophosphatidylcholine, its derivatives and/or procalcitonin as well as at least one clinical marker (e.g. temperature or respiration rate) and/or at least one biomarker for the early detection of sepsis in a subject.Type: GrantFiled: April 2, 2009Date of Patent: March 11, 2014Assignee: Becton, Dickinson and CompanyInventors: Song Shi, Richard L. Moore, James Garrett
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Publication number: 20140065714Abstract: Provided is a method and device for identifying glycated protein in a sample using a matrix including a boronic acid moiety that binds glycated proteins.Type: ApplicationFiled: March 18, 2013Publication date: March 6, 2014Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Sang-kyu KIM, Sung-ha PARK, Kyung-mi SONG, Soo-suk LEE, Youn-suk CHOI
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Publication number: 20140065716Abstract: A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.Type: ApplicationFiled: November 8, 2013Publication date: March 6, 2014Applicant: MOMENTA PHARMACEUTICALS, INC.Inventors: Ian Christopher Parsons, Ting Zheng, Nur Sibel Gunay, Carlos J. Bosques
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Publication number: 20140024124Abstract: A glycoprotein and/or a glycopeptide which are a test substance is heated in the presence of a pyrazolone derivative, an isoxazolone derivative, a hydantoin derivative, a rhodanine derivative, a maleimide derivative, or the like under a basic condition to cleave and label a post-translational modification group for analysis, thereby enabling analysis of a post-translational modification of a serine residue and/or a threonine residue.Type: ApplicationFiled: February 16, 2012Publication date: January 23, 2014Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, CELLSEED INC.Inventors: Yasuro Shinohara, Yasuhiro Takegawa, Naoki Fujitani, Jun-ichi Furukawa, Hideaki Sakai
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Patent number: 8628977Abstract: Reagents and methods are provided that permit simultaneous analysis of multiple diverse small molecule analytes present in a complex mixture. Samples are labeled with chemically identical but isotopically distinct forms of the labeling reagent, and analyzed using mass spectrometry. A single reagent simultaneously derivatizes multiple small molecule analytes having different reactive functional groups.Type: GrantFiled: May 1, 2009Date of Patent: January 14, 2014Assignee: Purdue Research FoundationInventors: Jiri Adamec, Wen-Chu Yang, Fred E. Regnier
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Patent number: 8623654Abstract: An object of the present invention is to provide a control material which can be preferably employed in detection of 1,5-anhydro-D-glucitol and glycoalbumin, which are employed as excellent indices for diabetes. The present inventor has found that mannitol which is added to serum or plasma used as control material stabilizes 1,5-anhydro-D-glucitol and glycoalbumin present in the serum or plasma for a long period of time, and that the object can be attained through provision of (1) an agent for stabilizing control material, the agent being composed of mannitol and (2) control material containing mannitol and 1,5-anhydro-D-glucitol.Type: GrantFiled: March 4, 2010Date of Patent: January 7, 2014Assignee: BML, Inc.Inventor: Isami Tsuboi
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Publication number: 20130344524Abstract: In a method for detecting a glucagon-secretin family peptide in a sample, the sample is contacted with a cleaving agent capable of digesting the glucagon-secretin family peptide by cleaving a peptide bond of at least one aspartic acid within the glucagon-secretin family peptide and thereby generating a plurality of peptide fragments, at least one of the peptide fragments containing an N-terminal end of the glucagon-secretin family peptide. The peptide fragment that contains the N-terminal end of the glucagon-secretin family peptide is then detected using liquid chromatography and mass spectrometry. The amount of peptide fragment that contains the N-terminal end of the glucagon-secretin family peptide in the sample can then be quantitated using a calibration curve.Type: ApplicationFiled: March 7, 2012Publication date: December 26, 2013Applicant: SANWA KAGAKU KENKYUSHO CO., LTD.Inventors: Atsushi Miyachi, Yoshika Yasuda, Hiroko Hirooka
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Publication number: 20130344511Abstract: The invention provides, in certain embodiments, a method of detecting an indicator of renal injury or renal disease. The method entails assaying a urine sample for hematopoietic growth factor inducible neurokinin-1 (HGFIN), wherein the presence of HGFIN at an elevated level indicates the presence and/or degree of renal injury or renal disease, and/or the rate of loss of renal function. In other embodiments, the invention provides a method of detecting an indicator of systemic inflammation. This method entails assaying a biological sample for HGFIN, wherein the presence of HGFIN at an elevated level indicates the presence and/or degree of systemic inflammation. Also provided, are methods of determining progression of these conditions, as well as methods of determining subjects' response to treatment.Type: ApplicationFiled: June 10, 2013Publication date: December 26, 2013Inventors: Sharon ADLER, Tiane DAI, Ying WANG, Mukti PATEL-CHAMBERLIN, Cynthia C. NAST, Janine LaPAGE, Nosratolah Dabir VAZIRI, Madeleine V. PAHL
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Publication number: 20130337536Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.Type: ApplicationFiled: August 21, 2013Publication date: December 19, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Evan Antoulinakis, Kyle Gee, Aleksey Rukavishnikov
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Patent number: RE45074Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: October 16, 2012Date of Patent: August 12, 2014Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori