Peptide, Protein Or Amino Acid Patents (Class 436/86)
  • Patent number: 11181529
    Abstract: The three-dimensional structural analysis of pharmaceutical and/or biological molecules is performed by the reaction of OH radicals on the surfaces of the molecules of interest. Quantitation and/or completeness of the OH radicals are optionally measured using buffers intrinsic to the sample solutions as internal standards. Measurements of the reactions of these buffers with OH radicals provide an internal standard while avoiding the use of prior art internal standards that can have unwanted effects on the three-dimensional structures of interest.
    Type: Grant
    Filed: March 5, 2021
    Date of Patent: November 23, 2021
    Assignee: GenNext Technologies, Inc.
    Inventors: Scot Randy Weinberger, Joshua S. Sharp, Sandeep Misra
  • Patent number: 11175271
    Abstract: A highly-sensitive-allergen-measurement method is provided. A method for detecting an allergen in a sample comprises treating the sample with a protease, and detecting the presence or absence of an allergen-derived polypeptide in the enzymatically treated sample by a chromatographic separation analysis, wherein the allergen is one or more members selected from the group consisting of buckwheat, crustacean, milk, egg and peanut.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: November 16, 2021
    Assignee: NISSHIN SEIFUN GROUP INC.
    Inventor: Yusuke Seki
  • Patent number: 11174568
    Abstract: A microfluidic chip comprising at least one dialysis crystallisation cell. The cell includes: a substrate made of PMMA; a first level including a tank defined at least partially by the substrate and by an outer wall of the cell, the tank being in fluid communication with a channel for inlet and a channel for outlet of a solution allowing the crystallisation method to be implemented; and a second level including a dialysis chamber defined at least partially by an inner wall of the cell without contact with the substrate and by a dialysis membrane forming an interface between the tank and the dialysis chamber, the inner wall including at least one one-piece portion in which the periphery of the membrane is kept sealed.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: November 16, 2021
    Assignees: UNIVERSITE GRENOBLE ALPES, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES, LTERNATIVES CENTRE NATIONAL DE LA RECHERCHE, SCIENTIFIQUE RHODIA OPERATIONS, RHODIA LABORATOIRE DU FUTUR, UNIVERSITE DE BORDEAUX
    Inventors: Monika Spano, Jean-Baptiste Salmon, Niels Heinz Junius
  • Patent number: 11162952
    Abstract: The disclosure concerns methods for identifying amino acids in peptides, including peptides including unnatural amino acids. Various aspects of the present disclosure provide compositions and methods for amino acid-type specific labeling, as well as methods for detecting the labels. Further disclosed herein are strategies for highly multiplexed, high-throughput peptide analysis.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: November 2, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
  • Patent number: 11156559
    Abstract: A test device is configured for diagnostic testing and includes an optical readable medium, in turn including a pattern of spots of material arranged on a surface of the device. Several patterns may be provided. The patterns accordingly formed may be human and/or machine readable. They may notably encode security information, e.g., indicating whether the device has already been used. The spots may notably be inkjet spotted. In addition, a method is provided for decoding information encoded in a pattern of such a test device. In embodiments, liquid is introduced in the device, which comprises additional spots having a substantially different solubility than spots forming the actual pattern. Thus, the additional spots get solubilized in and flushed by the liquid as the latter wets them, and an initially hidden pattern may be read, which is formed of the remaining spots (not solubilized). Encoding methods are also provided.
    Type: Grant
    Filed: July 6, 2019
    Date of Patent: October 26, 2021
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Onur Goekce, Yuksel Temiz
  • Patent number: 11139049
    Abstract: A method comprising causing a simulation machine for molecular dynamic simulation to determine that a topological distance that separates two particles is less than a threshold. The simulation machine includes nodes connected by a network. The nodes collectively representing a volume with each node corresponding to a portion of the simulation space. A topological relationship between the nodes corresponds to spatial relationship thereof in the simulation space. The simulation volume is occupied by particles that interact with each other. The two particles are among these particles. The simulation volume includes node boxes, each of which is handled by one of the nodes. Each of the nodes is implemented as an application specific integrated circuit that includes a combination of first and second hardware elements. The first hardware elements are especially designed to perform pairwise interactions. The second hardware elements operate to provide potentially interacting particles to the first hardware elements.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: October 5, 2021
    Assignee: D.E. Shaw Research, LLC
    Inventors: Ping Tak Peter Tang, J. P. Grossman, Brannon Batson, Ron Dror
  • Patent number: 11105812
    Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: August 31, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric V. Anslyn
  • Patent number: 11059881
    Abstract: The inventors discovered that viscosity of a protein solution can be estimated by measuring the apparent particle size or apparent molecular weight by a small angle X-ray scattering (SAXS) method or X-ray solution scattering method, which enables measurement of small amounts of samples, and then correlating those measurement results with viscosity of the protein solution.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: July 13, 2021
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Masakazu Fukuda, Akira Hayasaka, Tomoyuki Igawa, Atsuhiko Maeda, Machiko Fujino
  • Patent number: 10983108
    Abstract: Methods are described for diagnosing or prognosing insulin resistance in diabetic and pre-diabetic patients, the method comprising determining the amount of insulin and C-peptide in a sample. Provided herein are mass spectrometric methods for detecting and quantifying insulin and C-peptide in a biological sample utilizing enrichment and/or purification methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: April 20, 2021
    Assignee: Quest Diagnostics Investments LLC
    Inventors: Dov Shiffman, Carmen Tong, James J. Devlin, Michael J. McPhaul
  • Patent number: 10942187
    Abstract: A method for selectively and easily quantifying the L-form and/or D-form amino acids to be measured using an aminoacyl tRNA synthetase (AARS) with high sensitivity, and an amino acid quantification kit. A method for quantifying amino acids (L-AA and/or D-AA) in a sample using an AARS, wherein the amino acids and the AARS are released from an aminoacyl AMP-AARS complex once formed, and they are used again for forming the aminoacyl AMP-AARS complex, so that reaction products such as pyrophosphoric acid to be measured can be ultimately produced up to a molar number larger than that of the amino acids contained in the sample, and an amino acid quantification kit for performing the method.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: March 9, 2021
    Assignees: IKEDA FOOD RESEARCH CO., LTD., HIROSHIMA CITY UNIVERSITY
    Inventors: Daisuke Sato, Tomoko Nakatsuka, Hideyuki Aoki, Mikiko Kida, Kenta Yamada, Shoji Kaneko, Akimitsu Kugimiya
  • Patent number: 10890582
    Abstract: A sensor for detecting an analyte can include a photoluminescent nanostructure embedded in a sensor hydrogel. The sensor hydrogel can be supported by a substrate hydrogel.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: January 12, 2021
    Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Nigel F. Reuel, Michael S. Strano
  • Patent number: 10858643
    Abstract: An approach for preparing a vaccine using ultraviolet radiation is described. Aspects of this approach involve multiple iterations of inactivation of the vaccine using an ultraviolet radiation source at a set of different wavelengths and dosages. A recognition test of the vaccine using the set of different wavelengths and dosages is performed after the multiple iterations of inactivation. A controller compares results from the inactivation test and the recognition test to determine an area of acceptable radiation dosages and wavelengths generated from the ultraviolet radiation source that irradiate the live organisms without affecting efficacy and safety of the vaccine. The area of acceptable ultraviolet radiation dosages and wavelengths is representative of a difference between an ultraviolet radiation dosage that is required for inactivation and an ultraviolet radiation dosage that leads to a loss of recognition.
    Type: Grant
    Filed: October 31, 2016
    Date of Patent: December 8, 2020
    Assignee: Sensor Electronic Technology, Inc.
    Inventors: Alexander Dobrinsky, Michael Shur
  • Patent number: 10835148
    Abstract: Particular aspects of the invention are methods for assaying metabolite levels in samples from a patient during pregnancy using nuclear magnetic resonance and direct flow injection mass spectrometry. In various methods, the assayed metabolites may be acylcarnitine or one or more of C3-OH (hydroxypropionylcarnitine), C5-OH (C3DC), C10, C5:1-DC (glutaconylcarnitine), C14:1-OH (hydroxytetradecenoylcarnitine) and C14:2-OH. One or more methods also may include measuring nuchal translucency of the fetus. Other methods relate to predicting fetal congenital heart defects in a fetus.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: November 17, 2020
    Assignee: Bioscreening & Diagnostics LLC
    Inventors: Ray O. Bahado-Singh, Kypros Nicolaides
  • Patent number: 10802089
    Abstract: Provided are magnetic sensors, which include a magnetic sensor element having a sensor surface modification and an inter-element area adjacent to the magnetic sensor element and having an inter-element area surface modification, where the sensor surface modification and the inter-element area surface modification provide a binding surface in the inter-element area. Also provided are devices, systems and methods in which the subject magnetic sensors find use.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: October 13, 2020
    Assignee: MagArray, Inc.
    Inventors: Sebastian J. Osterfeld, Heng Yu
  • Patent number: 10793890
    Abstract: An apparatus (1000) and kit (1000) for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having (100) a sampling portion (30) and a handling portion (20). The sampling portion (30) is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity (32) that is not substantially defined by space between a plurality of fibers. The sampling device (30) comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: October 6, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Kenneth R. Davenport, Mark B. Driscoll, Rachel E. Evans, Catherine M. Ramsay, Neil Percy
  • Patent number: 10724950
    Abstract: Disclosed is a method for preparing dispersion gradients and an SPR injection method for determining full kinetics and affinity analysis in the presence of a competitor molecule. The SPR injection provides a dispersion gradient of two or more samples to a SPR flow cell and detector.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: July 28, 2020
    Assignee: SARTORIUS BIOANALYTICAL INSTRUMENTS, INC.
    Inventor: Aaron David Martin
  • Patent number: 10724939
    Abstract: A disposable rapid cell sorter comprises a microfluidic chip with electrodes and sorts biological cells of interest though a magnetic field and an electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: July 28, 2020
    Assignee: Cytek Biosciences, Inc.
    Inventor: Wenbin Jiang
  • Patent number: 10632401
    Abstract: A water filtration device comprising: a diffusiophoretic water filter having at least one channel having an inlet and an outlet and for receiving a colloidal suspension at the inlet and flowing the colloidal suspension between the inlet and the outlet in a flow direction, the channel being in contact with a diffusiophoretic-inducing membrane. Other devices and methods are also disclosed.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: April 28, 2020
    Assignee: Split Rock Filter Systems LLC
    Inventor: William C. Gehris
  • Patent number: 10627401
    Abstract: The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.
    Type: Grant
    Filed: September 21, 2015
    Date of Patent: April 21, 2020
    Assignee: University of Utah Research Foundation
    Inventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
  • Patent number: 10591448
    Abstract: The present disclosure relates to structural elucidation of isotopically labeled analytes using chromatography-MS and data-independent acquisition. The present disclosure can be used for rapid screening of analytes, such as the metabolome, including the simultaneous collection of both qualitative and quantitative information of known and unknown analytes.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: March 17, 2020
    Assignee: Waters Technologies Corporation
    Inventor: Giuseppe Astarita
  • Patent number: 10539568
    Abstract: The present invention relates to reversed phase high-performance liquid chromatography (HPLC) methods useful for the characterisation of glatiramer acetate or similar polypeptide mixtures. The method described herein can distinguish glatiramer acetate from non-conforming copolymers and may be used to choose batches of glatiramer acetate suitable for pharmaceutical use.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: January 21, 2020
    Assignee: CHEMI, S.P.A.
    Inventors: Maria Lattanzio, Aureliano Gaiassi, Andrea Stevenazzi
  • Patent number: 10520486
    Abstract: Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: December 31, 2019
    Assignees: National University Corporation Kyoto Institute of Technology, NOF Corporation
    Inventors: Yoichi Kumada, Yasutaka Kawaguchi, Aranna Nemoto, Fumio Nakashima, Nobuyuki Sakamoto
  • Patent number: 10520471
    Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: December 31, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Patent number: 10434134
    Abstract: The invention is directed to peptides which inhibit phosphorylation of an amino phosphoacceptor domain of C/EBP? and their use for treating inflammation and fibrosis.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: October 8, 2019
    Assignee: The Regents of the University of California
    Inventors: Mario Chojkier, Martina Buck
  • Patent number: 10401366
    Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: September 3, 2019
    Assignee: Life Technologies Corporation
    Inventors: Levente Egry, Kyle Gee
  • Patent number: 10337985
    Abstract: Provided is a measurement system for performing qualitative measurement and quantitative measurement of a measurement item of a biological sample, the measurement system including a quantitative sample adjustment criterion storage section that stores a quantitative sample adjustment criterion corresponding to a qualitative measurement result in the qualitative measurement, a determination section that determines a proper quantitative sample adjustment condition by referring to the quantitative sample adjustment criterion to determine necessity to change the quantitative sample adjustment condition based on the qualitative measurement result, and an adjustment section that adjusts the biological sample based on the quantitative sample adjustment condition determined by the determination section.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: July 2, 2019
    Assignee: ARKRAY, Inc.
    Inventor: Shinya Nakajima
  • Patent number: 10330684
    Abstract: This disclosure relates generally to detection and, in particular, to detecting objects within a sample or fraction thereof.
    Type: Grant
    Filed: April 5, 2018
    Date of Patent: June 25, 2019
    Assignee: RareCyte, Inc.
    Inventors: Daniel E. Campton, Arturo B. Ramirez, Melinda R. Duplessis
  • Patent number: 10269107
    Abstract: A field image is formed using a tristimulus color model and used to detect target plants or entities on a field. Through the use of a luminance parameter floor, hue and saturation selection steps, feature recognition, a sizing floor and an aspect ratio ceiling, a very fast way is devised to recognize a target plant without need for consulting plant attribute databases, or to analyze spectral or other specialized data for comparison with known attributes. This allows a low calculational processing load and use of simple hardware such as a single board computer to handle machine vision in real time. Treatment steps can include a spray event, a light treatment, and a thermal/mechanical trauma.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: April 23, 2019
    Assignee: Global Neighbor Inc
    Inventors: Jonathan A Jackson, Norman Novotney, Christopher Carter, Austin Erdman, Patrick A Jackson
  • Patent number: 10267785
    Abstract: Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with the first and second volumes of fluid, and means for applying an electric potential difference between the first and second volumes of fluid. In some such embodiments, a conjugate product is provided which includes charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving the product in the first volume of fluid, the product is directed into the orifice.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: April 23, 2019
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Stuart Lindsay, Peiming Zhang
  • Patent number: 10241117
    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: March 26, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Erin E. Chambers, Martha Stapels, Joanne Mather
  • Patent number: 10211683
    Abstract: An authentication of a power transmitting unit (PTU) includes determining whether an access right to an external device is present in a power receiving unit (PRU) based on identification information of the PRU, and network-connecting the PRU to the external device in response to a result of the determining being that the access right is present in the PRU.
    Type: Grant
    Filed: July 22, 2014
    Date of Patent: February 19, 2019
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Nam Yun Kim, Sang Wook Kwon, Dong Zo Kim, Bong Chul Kim, Yun Kwon Park, Chi Hyung Ahn, Byoung Hee Lee
  • Patent number: 10126304
    Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: November 13, 2018
    Assignee: George Mason Research Foundation, Inc.
    Inventors: Alessandra Luchini, Lance Liotta, Virginia Espina
  • Patent number: 10067160
    Abstract: Methods, systems, and devices are described which facilitate mechanosynthesis through the sequential use of a plurality of tips, each of which may have a different affinity for feedstock, thereby allowing tip to tip transfers which enhance system versatility and reduce equipment complexity.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: September 4, 2018
    Assignee: CBN Nano Technologies, Inc.
    Inventors: Ralph C. Merkle, Robert A. Freitas, Jr., Jeremy Barton, Aru Hill, Michael Drew, Damian Allis, Tait Takatani, Michael Shawn Marshall, Matthew Kennedy
  • Patent number: 9994892
    Abstract: The present invention relates to a novel method for detecting instantaneously a biomarker immobilized to a solid surface and related uses. The method comprises exposing the biomarker to a probe having a magnetic label in a solution; applying a magnetic field to the solution, whereby a complex of the biomarker and the probe is formed on the solid surface; withdrawing the magnetic field; removing the solution from the solid surface; and detecting the complex instantaneously, wherein the presence of the complex on the solid surface indicates the presence of the biomarker.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: June 12, 2018
    Inventor: Zhong Li
  • Patent number: 9964545
    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: May 8, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Erin E. Chambers, Martha Stapels, Joanne Mather
  • Patent number: 9964546
    Abstract: Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: May 8, 2018
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Zhaohui Chen
  • Patent number: 9835632
    Abstract: The present invention provides methods for predicting the likelihood of mucosal healing in an individual with inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis (UC). In addition, the present invention provides methods for monitoring the progression of mucosal healing in an individual with a disease such as IBD. Information on mucosal healing status derived from the use of the present invention can also aid in optimizing therapy and/or monitoring the therapeutic efficiency of an anti-TNF? inhibitor drug.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: December 5, 2017
    Assignee: Nestec S.A.
    Inventors: Sharat Singh, Severine Vermeire
  • Patent number: 9804172
    Abstract: The present invention relates to materials and methods concerning the IL-1 receptor family protein ST2. Use of soluble ST2 as a marker for cardiovascular disease or disease outcome is provided, in particular as a marker of the risk of mortality.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: October 31, 2017
    Assignees: INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), UNIVERSITE PARIS-EST CRETEIL VAL DE MARNE, ASSISTANCE PUBLIQUE—HOPITAUX DE PARIS
    Inventors: Yves Levy, Sophie Hue, Jean-Daniel Lelievre
  • Patent number: 9804079
    Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: October 31, 2017
    Assignee: ProteinSimple
    Inventor: Jiaqi Wu
  • Patent number: 9784744
    Abstract: The present invention relates to labeled chiral alpha-hydroxy ketoacid derivatives, a process for preparing the derivatives and their use for isotopic labeling of amino acids, in particular, for isotopic labeling of methyl groups of amino acids, and more particularly, for specific isotopic labeling of valine, leucine and isoleucine methyl groups, in proteins and biomolecular assemblies. The invention also concerns a process for analyzing proteins and biomolecular assemblies by NMR spectroscopy including a step of isotopic labeling of amino acids, in particular, valine, leucine and isoleucine, in proteins and biomolecular assemblies to be analyzed by the chiral alpha-hydroxy ketoacid derivatives of the invention. The invention further relates to a kit for isotopic labeling of valine, leucine and isoleucine amino acids, in proteins and biomolecular assemblies including one or more chiral alpha-hydroxy ketoacid derivatives of the invention.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: October 10, 2017
    Assignees: Commissariat a l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherch Scientifique
    Inventors: Michael Plevin, Rime Kerfah, Ombeline Pessey, Jérôme Boisbouvier, Pierre Gans
  • Patent number: 9753041
    Abstract: The present invention provides non-invasive diagnostic methods and kits for determining and/or monitoring oral health and glycemic control in subjects with Type 1 diabetes.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: September 5, 2017
    Assignee: UNIVERISTY OF SOUTH FLORIDA
    Inventors: Amy Christine Alman, Brant Roger Burkhardt
  • Patent number: 9720001
    Abstract: A method for detecting a list of known biopolymer molecules comprises: calculating, for each biopolymer, a respective list of oligomer molecules predicted to be produced by chemical processing; calculating, for each oligomer molecule, a respective predicted chromatographic elution time period; assigning, for each biopolymer molecule, one or more selected oligomer molecules to be detected, wherein the selecting is performed using weighted selection probabilities determined from the predicted elution times; scheduling a plurality of oligomer detection events of a detection system, wherein each oligomer detection event corresponds to a respective one of the predicted elution time periods; performing the chemical reaction or processing of the sample to generate a processed sample; introducing the processed sample into a chromatographic system; introducing any eluting oligomers into the detection system; and operating the detection system so as to search for each of the selected oligomer molecules in accordance wi
    Type: Grant
    Filed: May 21, 2014
    Date of Patent: August 1, 2017
    Assignee: THERMO FINNIGAN LLC
    Inventors: Robert A. Grothe, Jr., Iman Mohtashemi
  • Patent number: 9625469
    Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
    Type: Grant
    Filed: June 22, 2012
    Date of Patent: April 18, 2017
    Assignee: Board Of Regents, The University of Texas System
    Inventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric Anslyn
  • Patent number: 9606111
    Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: March 28, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Steve Freeby, Ning Liu, Kevin McDonald, Aran Paulus, Anton Posch
  • Patent number: 9597352
    Abstract: In the present invention, Applicants demonstrate the effect of a biomembrane sealing agent on the development of chronic pain following tissue injury as well as acute pain in a model of acute inflammation. Applicants demonstrate the ability of this class of agents referred to as “biomembrane sealing agents” to reduce the severity of hyperalgesia and allodynia following mechanical insult to the nervous system as well as their ability to reduce acute pain in a model of acute inflammation. Applicant describes the use of injectable or depot formulations of biomembrane sealing agent(s) for prophylactic treatment such as they could be administered after the insult (i.e. post-injury or post-surgery) but before the onset of acute or chronic pain. Alternatively, biomembrane sealing agents could be used to reduce the severity of acute or chronic pain after onset.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: March 21, 2017
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Josee Roy, Jeffrey C. Marx
  • Patent number: 9594074
    Abstract: Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques. The present invention provides methods for detecting the presence or amount of C peptide in a sample by mass spectrometry.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: March 14, 2017
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Zhaohui Chen
  • Patent number: 9574236
    Abstract: Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.
    Type: Grant
    Filed: January 6, 2015
    Date of Patent: February 21, 2017
    Assignee: Agilent Technologies, Inc.
    Inventors: Brian Phillip Smart, Robert A. Ach
  • Patent number: 9567626
    Abstract: A monolithic device comprises a substrate. An array of sensing elements is coupled to the substrate, and each sensing element includes a magnetoresistive sensor. An analog electric drive generator is coupled to the substrate, and the electric drive generator produces a sensor-biasing signal that biases the magnetoresistive sensors of the array. An analog multiplexer is coupled to the substrate, and outputs of the array are coupled to inputs of the multiplexer. An analog signal conditioning circuit is coupled to the substrate, wherein at least one output of the multiplexer is coupled to at least one input of the signal conditioning circuit. The monolithic device is fabricated using both complementary metal-oxide semiconductor (i.e., CMOS) technology and thin film technology. For example, the electric drive generator, the multiplexer, and the signal conditioning circuit may be fabricated with CMOS technology, while the magnetoresistive sensors of the array are fabricated with thin film technology.
    Type: Grant
    Filed: July 3, 2014
    Date of Patent: February 14, 2017
    Assignee: MAGNOMICS, S.A.
    Inventors: Filipe Arroyo Cardoso, Tiago Miguel Lopes Marta da Costa, José António Henriques Germano, Moisés Simões Piedade
  • Patent number: 9547013
    Abstract: This invention provides methods and biomarkers for diagnosing autism by identifying cellular metabolites differentially produced in autistic patient samples versus non-autistic controls. Methods for identifying a unique profile of metabolites present of secreted in brain tissue, cerebrospinal fluid, plasma, or biofluids of autistic samples are described herein. The individual metabolites or a pattern of secreted metabolites provide metabolic signatures of autism, which can be used to provide a diagnosis thereof.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: January 17, 2017
    Assignees: Wisconsin Alumni Research Foundation, Stemina Biomarker Discovery, Inc.
    Inventors: Gabriela Gebrin Cezar, Alan M. Smith
  • Patent number: 9448240
    Abstract: A thiol probe for a protein, which promotes ionization in proteomic analysis using mass spectrometry and can be used even for a protein that has a high degree of hydrophobicity and quickly turns over, and is represented by the following formula (I): In formula (I), R1 represents a linker group, and R2 represents a substituted ammonium group or a substituted amino group. A mass spectrometry method for a protein, includes the steps of obtaining a modified protein by reacting the thiol probe with a protein to be subjected to mass spectrometry and subjecting the modified protein to mass spectrometry.
    Type: Grant
    Filed: August 17, 2012
    Date of Patent: September 20, 2016
    Assignee: SHIMADZU CORPORATION
    Inventors: Takashi Shimada, Taka-Aki Sato, Koichi Tanaka