Peptide, Protein Or Amino Acid Patents (Class 436/86)
  • Patent number: 11499952
    Abstract: A process for quantifying the amount of unbound metal and bound metal in solution is provided. A process for quantifying the amount of bound metal amino acid chelate and free ligand in a solid (e.g., dry mixture such as an animal feed) is also provided.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: November 15, 2022
    Assignee: PREMEX, INC.
    Inventor: Paula Leon Monsalve
  • Patent number: 11493518
    Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: November 8, 2022
    Assignee: GEORGE MASON RESEARCH FOUNDATION, INC.
    Inventors: Alessandra Luchini Kunkel, Lance Liotta, Virginia Espina
  • Patent number: 11493514
    Abstract: A novel method of detecting and destroying viral transmissions such as SARS-CoV-2 transmission is described. The proposed technique uses a light source such as that from a smart phone and a mobile spectrophotometer to enable detection of proteins in solution. The technique allows for detecting soluble preparations of for example spike protein subunits from SARS-CoV-2, followed by detection of the actual binding potential of the spike protein with its host receptor, for example the angiotensin-converting enzyme 2 (ACE2) or other antigens or elements. The results are validated by showing that this method can detect antigen-antibody binding using two independent protein-antibody pairs. Finally, this technique is combined with DC bias to show that introduction of a current in the system can be used to disrupt the antigen-antibody reaction, suggesting that this technique can be a powerful means of disrupting virus transmission by destroying virus-receptor interactions.
    Type: Grant
    Filed: July 19, 2021
    Date of Patent: November 8, 2022
    Assignee: United Arab Emirates University
    Inventors: Mahmoud F. Y. Al Ahmad, Tahir A. Rizvi, Farah Mustafa
  • Patent number: 11485768
    Abstract: The invention relates to isolated immunogenic peptides comprising a CD1d binding peptide, and immediately adjacent or separated from said CD1d binding peptide, a redox motif sequence which is further flanked by a histidine or tryptophan amino acids. The invention further relates to these peptides for use as a medicament The invention further relates to methods wherein these peptides are used for generating NKT cells which are cytolytic against cells presenting the cognate antigen.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: November 1, 2022
    Assignee: IMCYSE SA
    Inventor: Luc Vander Elst
  • Patent number: 11486874
    Abstract: Methods are described for determining the amount of insulin in a sample. Provided herein are mass spectrometric methods for detecting and quantifying insulin and C-peptide in a biological sample utilizing enrichment and/or purification methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques. Also provided herein are mass spectrometric methods for detecting and quantifying insulin and b-chain in a biological sample utilizing enrichment and/or purification methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
    Type: Grant
    Filed: June 17, 2019
    Date of Patent: November 1, 2022
    Assignee: Quest Diagnostics Investments LLC
    Inventors: Steven W. Taylor, Michael McPhaul, Richard E. Reitz, Zhaohui Chen, Nigel Clarke
  • Patent number: 11435358
    Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
    Type: Grant
    Filed: October 1, 2021
    Date of Patent: September 6, 2022
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan
  • Patent number: 11414459
    Abstract: The present disclosure relates to compounds of Formula I being cyclic metabolites of oxytocin. These derivatives have enhanced selectivity for the oxytocin receptor.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: August 16, 2022
    Assignee: Kinoxis Therapeutics Pty Ltd
    Inventors: Michael Kassiou, Damien Gulliver, Tristan Reekie, Timothy Katte, William Jorgensen, Eryn Werry
  • Patent number: 11416998
    Abstract: A method to process a contributing digital image of a subject in an image-processing computer. The contributing digital image is received in a depth-resolving machine configured to furnish a depth image based at least in part on the contributing digital image. The contributing digital image is also received in a classification machine previously trained to classify a pixel of the contributing digital image as liable to corrupt a depth value of a corresponding pixel of the depth image. A repair value is computed for the depth value of the corresponding pixel of the depth image, which is then corrected based on the repair value and returned to the calling process.
    Type: Grant
    Filed: July 30, 2019
    Date of Patent: August 16, 2022
    Assignee: Microsoft Technology Licensing, LLC
    Inventors: Michael Bleyer, Yuri Pekelny, Raymond Kirk Price
  • Patent number: 11408897
    Abstract: The use of mass defect signatures to impart milliDalton mass differences between isotopically labeled peptides at the MS1-level allows multiplex quantification without the increased mass spectral complexity that occurs with mass difference approaches. Provided herein is a mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is compact and easy to synthesize at high purity in few steps using commercially available starting materials. The multiplex DiPyrO tags are amine-reactive and can impart a mass difference onto labeled peptides and through calculated substitution of heavy isotopes. DiPyrO offers up to 10-plex quantification on current Orbitrap or FT-ICR platforms without increasing mass spectral complexity. The synthesis of the DiPyrO tag is provided along with viability of the DiPyrO tag for labeling complex proteomics samples using yeast extract digests and its effect on labeled peptides during LC-MS2 analysis.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: August 9, 2022
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Lingjun Li, Dustin Frost, Amanda Buchberger Jones
  • Patent number: 11400448
    Abstract: The disclosure provides a chip substrate and a digital micro-fluidic chip and belongs to the field of digital micro-fluidic technology. The chip substrate provided by the disclosure has a plurality of control regions spaced apart from each other, the chip substrate including: a first base substrate; a driving electrode disposed in each control region over the first base substrate, the driving electrode being configured to drive a droplet to move, wherein the chip substrate further comprises a pressure detecting element provided in each control region over the first base substrate, and configured to detect a pressure from the droplet, so that the chip substrate determines a position of the droplet according to the pressure.
    Type: Grant
    Filed: March 19, 2019
    Date of Patent: August 2, 2022
    Assignees: BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD., BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Yue Geng, Peizhi Cai, Fengchun Pang, Le Gu, Chuncheng Che
  • Patent number: 11402328
    Abstract: The present invention relates to a method for detecting an amyloid ? oligomer which includes a test sample and thioflavin T are brought into contact with each other, fluorescence of the thioflavin T is measured to obtain time-resolved fluorescence spectra, the time-resolved fluorescence spectrum of time period t1 to t2 and the time-resolved fluorescence spectrum of time period t3 to t4 are respectively normalized to obtain normalized spectra (t1<t2?t3<t4), and determination is made to confirm the presence or absence of an amyloid ? oligomer in the test sample on the basis of the two normalized spectra, and shifting of the normalized spectrum of time period t3 to t4 towards the low wavelength side in comparison to the normalized spectrum of time period t1 to t2 indicates the presence of an amyloid ? oligomer in the test sample.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: August 2, 2022
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Akinori Oda, Hiroshi Satozono, Tomomi Shinke, Yohei Takata
  • Patent number: 11397188
    Abstract: Certain embodiments are directed to marker peptides or marker peptide antibodies can be used in producing diagnostic kits or used in diagnostic methods for Alzheimer's disease. The antibodies and/or marker peptides can be used in immunohistochemical and biochemical methods for qualitative and quantitative analysis of marker peptide levels and/or localization in brain samples and CSF samples.
    Type: Grant
    Filed: March 29, 2018
    Date of Patent: July 26, 2022
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventor: Krishna Bhat
  • Patent number: 11396527
    Abstract: Described are dual mass-spectrometry-cleavable cross-linkers that can be cleaved selectively using two differential tandem mass-spectrometric techniques such as collision induced dissociation (CID) or electron transfer dissociation (ETD), i.e., a dual cleavable crosslinking technology (DUCCT) cross-linker. When used to cross-link a macromolecule, such as a peptide, MS/MS fragmentation produces two signature complementary mass spectra of same cross-linked peptides, the analysis of which gives rise to high confidence in characterizing the structures of the cross-linked macromolecules as well as sites of interactions. Also described, are methods of making and using DUCCT cross-linkers.
    Type: Grant
    Filed: July 8, 2020
    Date of Patent: July 26, 2022
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventor: Saiful Mahmud Chowdhury
  • Patent number: 11339365
    Abstract: Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be translocated. The exemplified unfoldase is the ClpX unfoldase from E. coli.
    Type: Grant
    Filed: February 15, 2013
    Date of Patent: May 24, 2022
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Jeffrey Matthew Nivala, Douglas Benjamin Marks, Mark Albert Akeson
  • Patent number: 11237102
    Abstract: The disclosure relates to methods and systems for the analysis of compounds in a crystalline state and/or undergoing crystallization. Two-dimensional correlation (2DCOS) and co-distribution analysis (2DCDS) analysis plots can be generated and analyzed. Asynchronous plots can aid in establishing a sequential order of events. Positive cross peaks that correlate with auto peaks associated with aggregation can be identified. The auto peaks can be referenced to quickly discern the regions of the molecule most perturbed, which would indicate a driver for the crystallization state of the molecule. One can define which functional group types (e.g., region) are most perturbed (positive, intense auto peak) and observe how the different auto peaks begin to have greatest intensity change. These changes in auto peaks in the synchronous plots for the different stages of crystallization can provide information as to the dynamics of the process from amorphous to crystalline state.
    Type: Grant
    Filed: June 12, 2018
    Date of Patent: February 1, 2022
    Assignee: Protein Dynamic Solutions, Inc.
    Inventor: Belinda Pastrana-Rios
  • Patent number: 11199548
    Abstract: Methods for determining an in vitro release profile of peanut allergens in a sample are provided. Methods for determining one or more signatures of peanut allergens in a sample are provided.
    Type: Grant
    Filed: September 24, 2019
    Date of Patent: December 14, 2021
    Assignee: SANOFI
    Inventors: Kim Alving, Pierre deMontigny, Bing Huang Wang
  • Patent number: 11181529
    Abstract: The three-dimensional structural analysis of pharmaceutical and/or biological molecules is performed by the reaction of OH radicals on the surfaces of the molecules of interest. Quantitation and/or completeness of the OH radicals are optionally measured using buffers intrinsic to the sample solutions as internal standards. Measurements of the reactions of these buffers with OH radicals provide an internal standard while avoiding the use of prior art internal standards that can have unwanted effects on the three-dimensional structures of interest.
    Type: Grant
    Filed: March 5, 2021
    Date of Patent: November 23, 2021
    Assignee: GenNext Technologies, Inc.
    Inventors: Scot Randy Weinberger, Joshua S. Sharp, Sandeep Misra
  • Patent number: 11175271
    Abstract: A highly-sensitive-allergen-measurement method is provided. A method for detecting an allergen in a sample comprises treating the sample with a protease, and detecting the presence or absence of an allergen-derived polypeptide in the enzymatically treated sample by a chromatographic separation analysis, wherein the allergen is one or more members selected from the group consisting of buckwheat, crustacean, milk, egg and peanut.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: November 16, 2021
    Assignee: NISSHIN SEIFUN GROUP INC.
    Inventor: Yusuke Seki
  • Patent number: 11174568
    Abstract: A microfluidic chip comprising at least one dialysis crystallisation cell. The cell includes: a substrate made of PMMA; a first level including a tank defined at least partially by the substrate and by an outer wall of the cell, the tank being in fluid communication with a channel for inlet and a channel for outlet of a solution allowing the crystallisation method to be implemented; and a second level including a dialysis chamber defined at least partially by an inner wall of the cell without contact with the substrate and by a dialysis membrane forming an interface between the tank and the dialysis chamber, the inner wall including at least one one-piece portion in which the periphery of the membrane is kept sealed.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: November 16, 2021
    Assignees: UNIVERSITE GRENOBLE ALPES, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES, LTERNATIVES CENTRE NATIONAL DE LA RECHERCHE, SCIENTIFIQUE RHODIA OPERATIONS, RHODIA LABORATOIRE DU FUTUR, UNIVERSITE DE BORDEAUX
    Inventors: Monika Spano, Jean-Baptiste Salmon, Niels Heinz Junius
  • Patent number: 11162952
    Abstract: The disclosure concerns methods for identifying amino acids in peptides, including peptides including unnatural amino acids. Various aspects of the present disclosure provide compositions and methods for amino acid-type specific labeling, as well as methods for detecting the labels. Further disclosed herein are strategies for highly multiplexed, high-throughput peptide analysis.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: November 2, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
  • Patent number: 11156559
    Abstract: A test device is configured for diagnostic testing and includes an optical readable medium, in turn including a pattern of spots of material arranged on a surface of the device. Several patterns may be provided. The patterns accordingly formed may be human and/or machine readable. They may notably encode security information, e.g., indicating whether the device has already been used. The spots may notably be inkjet spotted. In addition, a method is provided for decoding information encoded in a pattern of such a test device. In embodiments, liquid is introduced in the device, which comprises additional spots having a substantially different solubility than spots forming the actual pattern. Thus, the additional spots get solubilized in and flushed by the liquid as the latter wets them, and an initially hidden pattern may be read, which is formed of the remaining spots (not solubilized). Encoding methods are also provided.
    Type: Grant
    Filed: July 6, 2019
    Date of Patent: October 26, 2021
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Onur Goekce, Yuksel Temiz
  • Patent number: 11139049
    Abstract: A method comprising causing a simulation machine for molecular dynamic simulation to determine that a topological distance that separates two particles is less than a threshold. The simulation machine includes nodes connected by a network. The nodes collectively representing a volume with each node corresponding to a portion of the simulation space. A topological relationship between the nodes corresponds to spatial relationship thereof in the simulation space. The simulation volume is occupied by particles that interact with each other. The two particles are among these particles. The simulation volume includes node boxes, each of which is handled by one of the nodes. Each of the nodes is implemented as an application specific integrated circuit that includes a combination of first and second hardware elements. The first hardware elements are especially designed to perform pairwise interactions. The second hardware elements operate to provide potentially interacting particles to the first hardware elements.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: October 5, 2021
    Assignee: D.E. Shaw Research, LLC
    Inventors: Ping Tak Peter Tang, J. P. Grossman, Brannon Batson, Ron Dror
  • Patent number: 11105812
    Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: August 31, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric V. Anslyn
  • Patent number: 11059881
    Abstract: The inventors discovered that viscosity of a protein solution can be estimated by measuring the apparent particle size or apparent molecular weight by a small angle X-ray scattering (SAXS) method or X-ray solution scattering method, which enables measurement of small amounts of samples, and then correlating those measurement results with viscosity of the protein solution.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: July 13, 2021
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Masakazu Fukuda, Akira Hayasaka, Tomoyuki Igawa, Atsuhiko Maeda, Machiko Fujino
  • Patent number: 10983108
    Abstract: Methods are described for diagnosing or prognosing insulin resistance in diabetic and pre-diabetic patients, the method comprising determining the amount of insulin and C-peptide in a sample. Provided herein are mass spectrometric methods for detecting and quantifying insulin and C-peptide in a biological sample utilizing enrichment and/or purification methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: April 20, 2021
    Assignee: Quest Diagnostics Investments LLC
    Inventors: Dov Shiffman, Carmen Tong, James J. Devlin, Michael J. McPhaul
  • Patent number: 10942187
    Abstract: A method for selectively and easily quantifying the L-form and/or D-form amino acids to be measured using an aminoacyl tRNA synthetase (AARS) with high sensitivity, and an amino acid quantification kit. A method for quantifying amino acids (L-AA and/or D-AA) in a sample using an AARS, wherein the amino acids and the AARS are released from an aminoacyl AMP-AARS complex once formed, and they are used again for forming the aminoacyl AMP-AARS complex, so that reaction products such as pyrophosphoric acid to be measured can be ultimately produced up to a molar number larger than that of the amino acids contained in the sample, and an amino acid quantification kit for performing the method.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: March 9, 2021
    Assignees: IKEDA FOOD RESEARCH CO., LTD., HIROSHIMA CITY UNIVERSITY
    Inventors: Daisuke Sato, Tomoko Nakatsuka, Hideyuki Aoki, Mikiko Kida, Kenta Yamada, Shoji Kaneko, Akimitsu Kugimiya
  • Patent number: 10890582
    Abstract: A sensor for detecting an analyte can include a photoluminescent nanostructure embedded in a sensor hydrogel. The sensor hydrogel can be supported by a substrate hydrogel.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: January 12, 2021
    Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Nigel F. Reuel, Michael S. Strano
  • Patent number: 10858643
    Abstract: An approach for preparing a vaccine using ultraviolet radiation is described. Aspects of this approach involve multiple iterations of inactivation of the vaccine using an ultraviolet radiation source at a set of different wavelengths and dosages. A recognition test of the vaccine using the set of different wavelengths and dosages is performed after the multiple iterations of inactivation. A controller compares results from the inactivation test and the recognition test to determine an area of acceptable radiation dosages and wavelengths generated from the ultraviolet radiation source that irradiate the live organisms without affecting efficacy and safety of the vaccine. The area of acceptable ultraviolet radiation dosages and wavelengths is representative of a difference between an ultraviolet radiation dosage that is required for inactivation and an ultraviolet radiation dosage that leads to a loss of recognition.
    Type: Grant
    Filed: October 31, 2016
    Date of Patent: December 8, 2020
    Assignee: Sensor Electronic Technology, Inc.
    Inventors: Alexander Dobrinsky, Michael Shur
  • Patent number: 10835148
    Abstract: Particular aspects of the invention are methods for assaying metabolite levels in samples from a patient during pregnancy using nuclear magnetic resonance and direct flow injection mass spectrometry. In various methods, the assayed metabolites may be acylcarnitine or one or more of C3-OH (hydroxypropionylcarnitine), C5-OH (C3DC), C10, C5:1-DC (glutaconylcarnitine), C14:1-OH (hydroxytetradecenoylcarnitine) and C14:2-OH. One or more methods also may include measuring nuchal translucency of the fetus. Other methods relate to predicting fetal congenital heart defects in a fetus.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: November 17, 2020
    Assignee: Bioscreening & Diagnostics LLC
    Inventors: Ray O. Bahado-Singh, Kypros Nicolaides
  • Patent number: 10802089
    Abstract: Provided are magnetic sensors, which include a magnetic sensor element having a sensor surface modification and an inter-element area adjacent to the magnetic sensor element and having an inter-element area surface modification, where the sensor surface modification and the inter-element area surface modification provide a binding surface in the inter-element area. Also provided are devices, systems and methods in which the subject magnetic sensors find use.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: October 13, 2020
    Assignee: MagArray, Inc.
    Inventors: Sebastian J. Osterfeld, Heng Yu
  • Patent number: 10793890
    Abstract: An apparatus (1000) and kit (1000) for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having (100) a sampling portion (30) and a handling portion (20). The sampling portion (30) is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity (32) that is not substantially defined by space between a plurality of fibers. The sampling device (30) comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: October 6, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Kenneth R. Davenport, Mark B. Driscoll, Rachel E. Evans, Catherine M. Ramsay, Neil Percy
  • Patent number: 10724950
    Abstract: Disclosed is a method for preparing dispersion gradients and an SPR injection method for determining full kinetics and affinity analysis in the presence of a competitor molecule. The SPR injection provides a dispersion gradient of two or more samples to a SPR flow cell and detector.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: July 28, 2020
    Assignee: SARTORIUS BIOANALYTICAL INSTRUMENTS, INC.
    Inventor: Aaron David Martin
  • Patent number: 10724939
    Abstract: A disposable rapid cell sorter comprises a microfluidic chip with electrodes and sorts biological cells of interest though a magnetic field and an electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: July 28, 2020
    Assignee: Cytek Biosciences, Inc.
    Inventor: Wenbin Jiang
  • Patent number: 10632401
    Abstract: A water filtration device comprising: a diffusiophoretic water filter having at least one channel having an inlet and an outlet and for receiving a colloidal suspension at the inlet and flowing the colloidal suspension between the inlet and the outlet in a flow direction, the channel being in contact with a diffusiophoretic-inducing membrane. Other devices and methods are also disclosed.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: April 28, 2020
    Assignee: Split Rock Filter Systems LLC
    Inventor: William C. Gehris
  • Patent number: 10627401
    Abstract: The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.
    Type: Grant
    Filed: September 21, 2015
    Date of Patent: April 21, 2020
    Assignee: University of Utah Research Foundation
    Inventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
  • Patent number: 10591448
    Abstract: The present disclosure relates to structural elucidation of isotopically labeled analytes using chromatography-MS and data-independent acquisition. The present disclosure can be used for rapid screening of analytes, such as the metabolome, including the simultaneous collection of both qualitative and quantitative information of known and unknown analytes.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: March 17, 2020
    Assignee: Waters Technologies Corporation
    Inventor: Giuseppe Astarita
  • Patent number: 10539568
    Abstract: The present invention relates to reversed phase high-performance liquid chromatography (HPLC) methods useful for the characterisation of glatiramer acetate or similar polypeptide mixtures. The method described herein can distinguish glatiramer acetate from non-conforming copolymers and may be used to choose batches of glatiramer acetate suitable for pharmaceutical use.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: January 21, 2020
    Assignee: CHEMI, S.P.A.
    Inventors: Maria Lattanzio, Aureliano Gaiassi, Andrea Stevenazzi
  • Patent number: 10520486
    Abstract: Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: December 31, 2019
    Assignees: National University Corporation Kyoto Institute of Technology, NOF Corporation
    Inventors: Yoichi Kumada, Yasutaka Kawaguchi, Aranna Nemoto, Fumio Nakashima, Nobuyuki Sakamoto
  • Patent number: 10520471
    Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: December 31, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Patent number: 10434134
    Abstract: The invention is directed to peptides which inhibit phosphorylation of an amino phosphoacceptor domain of C/EBP? and their use for treating inflammation and fibrosis.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: October 8, 2019
    Assignee: The Regents of the University of California
    Inventors: Mario Chojkier, Martina Buck
  • Patent number: 10401366
    Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: September 3, 2019
    Assignee: Life Technologies Corporation
    Inventors: Levente Egry, Kyle Gee
  • Patent number: 10337985
    Abstract: Provided is a measurement system for performing qualitative measurement and quantitative measurement of a measurement item of a biological sample, the measurement system including a quantitative sample adjustment criterion storage section that stores a quantitative sample adjustment criterion corresponding to a qualitative measurement result in the qualitative measurement, a determination section that determines a proper quantitative sample adjustment condition by referring to the quantitative sample adjustment criterion to determine necessity to change the quantitative sample adjustment condition based on the qualitative measurement result, and an adjustment section that adjusts the biological sample based on the quantitative sample adjustment condition determined by the determination section.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: July 2, 2019
    Assignee: ARKRAY, Inc.
    Inventor: Shinya Nakajima
  • Patent number: 10330684
    Abstract: This disclosure relates generally to detection and, in particular, to detecting objects within a sample or fraction thereof.
    Type: Grant
    Filed: April 5, 2018
    Date of Patent: June 25, 2019
    Assignee: RareCyte, Inc.
    Inventors: Daniel E. Campton, Arturo B. Ramirez, Melinda R. Duplessis
  • Patent number: 10269107
    Abstract: A field image is formed using a tristimulus color model and used to detect target plants or entities on a field. Through the use of a luminance parameter floor, hue and saturation selection steps, feature recognition, a sizing floor and an aspect ratio ceiling, a very fast way is devised to recognize a target plant without need for consulting plant attribute databases, or to analyze spectral or other specialized data for comparison with known attributes. This allows a low calculational processing load and use of simple hardware such as a single board computer to handle machine vision in real time. Treatment steps can include a spray event, a light treatment, and a thermal/mechanical trauma.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: April 23, 2019
    Assignee: Global Neighbor Inc
    Inventors: Jonathan A Jackson, Norman Novotney, Christopher Carter, Austin Erdman, Patrick A Jackson
  • Patent number: 10267785
    Abstract: Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with the first and second volumes of fluid, and means for applying an electric potential difference between the first and second volumes of fluid. In some such embodiments, a conjugate product is provided which includes charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving the product in the first volume of fluid, the product is directed into the orifice.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: April 23, 2019
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Stuart Lindsay, Peiming Zhang
  • Patent number: 10241117
    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: March 26, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Erin E. Chambers, Martha Stapels, Joanne Mather
  • Patent number: 10211683
    Abstract: An authentication of a power transmitting unit (PTU) includes determining whether an access right to an external device is present in a power receiving unit (PRU) based on identification information of the PRU, and network-connecting the PRU to the external device in response to a result of the determining being that the access right is present in the PRU.
    Type: Grant
    Filed: July 22, 2014
    Date of Patent: February 19, 2019
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Nam Yun Kim, Sang Wook Kwon, Dong Zo Kim, Bong Chul Kim, Yun Kwon Park, Chi Hyung Ahn, Byoung Hee Lee
  • Patent number: 10126304
    Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: November 13, 2018
    Assignee: George Mason Research Foundation, Inc.
    Inventors: Alessandra Luchini, Lance Liotta, Virginia Espina
  • Patent number: 10067160
    Abstract: Methods, systems, and devices are described which facilitate mechanosynthesis through the sequential use of a plurality of tips, each of which may have a different affinity for feedstock, thereby allowing tip to tip transfers which enhance system versatility and reduce equipment complexity.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: September 4, 2018
    Assignee: CBN Nano Technologies, Inc.
    Inventors: Ralph C. Merkle, Robert A. Freitas, Jr., Jeremy Barton, Aru Hill, Michael Drew, Damian Allis, Tait Takatani, Michael Shawn Marshall, Matthew Kennedy
  • Patent number: 9994892
    Abstract: The present invention relates to a novel method for detecting instantaneously a biomarker immobilized to a solid surface and related uses. The method comprises exposing the biomarker to a probe having a magnetic label in a solution; applying a magnetic field to the solution, whereby a complex of the biomarker and the probe is formed on the solid surface; withdrawing the magnetic field; removing the solution from the solid surface; and detecting the complex instantaneously, wherein the presence of the complex on the solid surface indicates the presence of the biomarker.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: June 12, 2018
    Inventor: Zhong Li