Peptide, Protein Or Amino Acid Patents (Class 436/86)
  • Patent number: 10802089
    Abstract: Provided are magnetic sensors, which include a magnetic sensor element having a sensor surface modification and an inter-element area adjacent to the magnetic sensor element and having an inter-element area surface modification, where the sensor surface modification and the inter-element area surface modification provide a binding surface in the inter-element area. Also provided are devices, systems and methods in which the subject magnetic sensors find use.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: October 13, 2020
    Assignee: MagArray, Inc.
    Inventors: Sebastian J. Osterfeld, Heng Yu
  • Patent number: 10793890
    Abstract: An apparatus (1000) and kit (1000) for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having (100) a sampling portion (30) and a handling portion (20). The sampling portion (30) is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity (32) that is not substantially defined by space between a plurality of fibers. The sampling device (30) comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: October 6, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Kenneth R. Davenport, Mark B. Driscoll, Rachel E. Evans, Catherine M. Ramsay, Neil Percy
  • Patent number: 10724950
    Abstract: Disclosed is a method for preparing dispersion gradients and an SPR injection method for determining full kinetics and affinity analysis in the presence of a competitor molecule. The SPR injection provides a dispersion gradient of two or more samples to a SPR flow cell and detector.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: July 28, 2020
    Assignee: SARTORIUS BIOANALYTICAL INSTRUMENTS, INC.
    Inventor: Aaron David Martin
  • Patent number: 10724939
    Abstract: A disposable rapid cell sorter comprises a microfluidic chip with electrodes and sorts biological cells of interest though a magnetic field and an electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: July 28, 2020
    Assignee: Cytek Biosciences, Inc.
    Inventor: Wenbin Jiang
  • Patent number: 10632401
    Abstract: A water filtration device comprising: a diffusiophoretic water filter having at least one channel having an inlet and an outlet and for receiving a colloidal suspension at the inlet and flowing the colloidal suspension between the inlet and the outlet in a flow direction, the channel being in contact with a diffusiophoretic-inducing membrane. Other devices and methods are also disclosed.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: April 28, 2020
    Assignee: Split Rock Filter Systems LLC
    Inventor: William C. Gehris
  • Patent number: 10627401
    Abstract: The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.
    Type: Grant
    Filed: September 21, 2015
    Date of Patent: April 21, 2020
    Assignee: University of Utah Research Foundation
    Inventors: Mark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
  • Patent number: 10591448
    Abstract: The present disclosure relates to structural elucidation of isotopically labeled analytes using chromatography-MS and data-independent acquisition. The present disclosure can be used for rapid screening of analytes, such as the metabolome, including the simultaneous collection of both qualitative and quantitative information of known and unknown analytes.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: March 17, 2020
    Assignee: Waters Technologies Corporation
    Inventor: Giuseppe Astarita
  • Patent number: 10539568
    Abstract: The present invention relates to reversed phase high-performance liquid chromatography (HPLC) methods useful for the characterisation of glatiramer acetate or similar polypeptide mixtures. The method described herein can distinguish glatiramer acetate from non-conforming copolymers and may be used to choose batches of glatiramer acetate suitable for pharmaceutical use.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: January 21, 2020
    Assignee: CHEMI, S.P.A.
    Inventors: Maria Lattanzio, Aureliano Gaiassi, Andrea Stevenazzi
  • Patent number: 10520486
    Abstract: Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: December 31, 2019
    Assignees: National University Corporation Kyoto Institute of Technology, NOF Corporation
    Inventors: Yoichi Kumada, Yasutaka Kawaguchi, Aranna Nemoto, Fumio Nakashima, Nobuyuki Sakamoto
  • Patent number: 10520471
    Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: December 31, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Patent number: 10434134
    Abstract: The invention is directed to peptides which inhibit phosphorylation of an amino phosphoacceptor domain of C/EBP? and their use for treating inflammation and fibrosis.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: October 8, 2019
    Assignee: The Regents of the University of California
    Inventors: Mario Chojkier, Martina Buck
  • Patent number: 10401366
    Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: September 3, 2019
    Assignee: Life Technologies Corporation
    Inventors: Levente Egry, Kyle Gee
  • Patent number: 10337985
    Abstract: Provided is a measurement system for performing qualitative measurement and quantitative measurement of a measurement item of a biological sample, the measurement system including a quantitative sample adjustment criterion storage section that stores a quantitative sample adjustment criterion corresponding to a qualitative measurement result in the qualitative measurement, a determination section that determines a proper quantitative sample adjustment condition by referring to the quantitative sample adjustment criterion to determine necessity to change the quantitative sample adjustment condition based on the qualitative measurement result, and an adjustment section that adjusts the biological sample based on the quantitative sample adjustment condition determined by the determination section.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: July 2, 2019
    Assignee: ARKRAY, Inc.
    Inventor: Shinya Nakajima
  • Patent number: 10330684
    Abstract: This disclosure relates generally to detection and, in particular, to detecting objects within a sample or fraction thereof.
    Type: Grant
    Filed: April 5, 2018
    Date of Patent: June 25, 2019
    Assignee: RareCyte, Inc.
    Inventors: Daniel E. Campton, Arturo B. Ramirez, Melinda R. Duplessis
  • Patent number: 10269107
    Abstract: A field image is formed using a tristimulus color model and used to detect target plants or entities on a field. Through the use of a luminance parameter floor, hue and saturation selection steps, feature recognition, a sizing floor and an aspect ratio ceiling, a very fast way is devised to recognize a target plant without need for consulting plant attribute databases, or to analyze spectral or other specialized data for comparison with known attributes. This allows a low calculational processing load and use of simple hardware such as a single board computer to handle machine vision in real time. Treatment steps can include a spray event, a light treatment, and a thermal/mechanical trauma.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: April 23, 2019
    Assignee: Global Neighbor Inc
    Inventors: Jonathan A Jackson, Norman Novotney, Christopher Carter, Austin Erdman, Patrick A Jackson
  • Patent number: 10267785
    Abstract: Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with the first and second volumes of fluid, and means for applying an electric potential difference between the first and second volumes of fluid. In some such embodiments, a conjugate product is provided which includes charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving the product in the first volume of fluid, the product is directed into the orifice.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: April 23, 2019
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Stuart Lindsay, Peiming Zhang
  • Patent number: 10241117
    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: March 26, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Erin E. Chambers, Martha Stapels, Joanne Mather
  • Patent number: 10211683
    Abstract: An authentication of a power transmitting unit (PTU) includes determining whether an access right to an external device is present in a power receiving unit (PRU) based on identification information of the PRU, and network-connecting the PRU to the external device in response to a result of the determining being that the access right is present in the PRU.
    Type: Grant
    Filed: July 22, 2014
    Date of Patent: February 19, 2019
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Nam Yun Kim, Sang Wook Kwon, Dong Zo Kim, Bong Chul Kim, Yun Kwon Park, Chi Hyung Ahn, Byoung Hee Lee
  • Patent number: 10126304
    Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: November 13, 2018
    Assignee: George Mason Research Foundation, Inc.
    Inventors: Alessandra Luchini, Lance Liotta, Virginia Espina
  • Patent number: 10067160
    Abstract: Methods, systems, and devices are described which facilitate mechanosynthesis through the sequential use of a plurality of tips, each of which may have a different affinity for feedstock, thereby allowing tip to tip transfers which enhance system versatility and reduce equipment complexity.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: September 4, 2018
    Assignee: CBN Nano Technologies, Inc.
    Inventors: Ralph C. Merkle, Robert A. Freitas, Jr., Jeremy Barton, Aru Hill, Michael Drew, Damian Allis, Tait Takatani, Michael Shawn Marshall, Matthew Kennedy
  • Patent number: 9994892
    Abstract: The present invention relates to a novel method for detecting instantaneously a biomarker immobilized to a solid surface and related uses. The method comprises exposing the biomarker to a probe having a magnetic label in a solution; applying a magnetic field to the solution, whereby a complex of the biomarker and the probe is formed on the solid surface; withdrawing the magnetic field; removing the solution from the solid surface; and detecting the complex instantaneously, wherein the presence of the complex on the solid surface indicates the presence of the biomarker.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: June 12, 2018
    Inventor: Zhong Li
  • Patent number: 9964545
    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: May 8, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Erin E. Chambers, Martha Stapels, Joanne Mather
  • Patent number: 9964546
    Abstract: Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: May 8, 2018
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Zhaohui Chen
  • Patent number: 9835632
    Abstract: The present invention provides methods for predicting the likelihood of mucosal healing in an individual with inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis (UC). In addition, the present invention provides methods for monitoring the progression of mucosal healing in an individual with a disease such as IBD. Information on mucosal healing status derived from the use of the present invention can also aid in optimizing therapy and/or monitoring the therapeutic efficiency of an anti-TNF? inhibitor drug.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: December 5, 2017
    Assignee: Nestec S.A.
    Inventors: Sharat Singh, Severine Vermeire
  • Patent number: 9804172
    Abstract: The present invention relates to materials and methods concerning the IL-1 receptor family protein ST2. Use of soluble ST2 as a marker for cardiovascular disease or disease outcome is provided, in particular as a marker of the risk of mortality.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: October 31, 2017
    Assignees: INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), UNIVERSITE PARIS-EST CRETEIL VAL DE MARNE, ASSISTANCE PUBLIQUE—HOPITAUX DE PARIS
    Inventors: Yves Levy, Sophie Hue, Jean-Daniel Lelievre
  • Patent number: 9804079
    Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: October 31, 2017
    Assignee: ProteinSimple
    Inventor: Jiaqi Wu
  • Patent number: 9784744
    Abstract: The present invention relates to labeled chiral alpha-hydroxy ketoacid derivatives, a process for preparing the derivatives and their use for isotopic labeling of amino acids, in particular, for isotopic labeling of methyl groups of amino acids, and more particularly, for specific isotopic labeling of valine, leucine and isoleucine methyl groups, in proteins and biomolecular assemblies. The invention also concerns a process for analyzing proteins and biomolecular assemblies by NMR spectroscopy including a step of isotopic labeling of amino acids, in particular, valine, leucine and isoleucine, in proteins and biomolecular assemblies to be analyzed by the chiral alpha-hydroxy ketoacid derivatives of the invention. The invention further relates to a kit for isotopic labeling of valine, leucine and isoleucine amino acids, in proteins and biomolecular assemblies including one or more chiral alpha-hydroxy ketoacid derivatives of the invention.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: October 10, 2017
    Assignees: Commissariat a l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherch Scientifique
    Inventors: Michael Plevin, Rime Kerfah, Ombeline Pessey, Jérôme Boisbouvier, Pierre Gans
  • Patent number: 9753041
    Abstract: The present invention provides non-invasive diagnostic methods and kits for determining and/or monitoring oral health and glycemic control in subjects with Type 1 diabetes.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: September 5, 2017
    Assignee: UNIVERISTY OF SOUTH FLORIDA
    Inventors: Amy Christine Alman, Brant Roger Burkhardt
  • Patent number: 9720001
    Abstract: A method for detecting a list of known biopolymer molecules comprises: calculating, for each biopolymer, a respective list of oligomer molecules predicted to be produced by chemical processing; calculating, for each oligomer molecule, a respective predicted chromatographic elution time period; assigning, for each biopolymer molecule, one or more selected oligomer molecules to be detected, wherein the selecting is performed using weighted selection probabilities determined from the predicted elution times; scheduling a plurality of oligomer detection events of a detection system, wherein each oligomer detection event corresponds to a respective one of the predicted elution time periods; performing the chemical reaction or processing of the sample to generate a processed sample; introducing the processed sample into a chromatographic system; introducing any eluting oligomers into the detection system; and operating the detection system so as to search for each of the selected oligomer molecules in accordance wi
    Type: Grant
    Filed: May 21, 2014
    Date of Patent: August 1, 2017
    Assignee: THERMO FINNIGAN LLC
    Inventors: Robert A. Grothe, Jr., Iman Mohtashemi
  • Patent number: 9625469
    Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
    Type: Grant
    Filed: June 22, 2012
    Date of Patent: April 18, 2017
    Assignee: Board Of Regents, The University of Texas System
    Inventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric Anslyn
  • Patent number: 9606111
    Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: March 28, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Steve Freeby, Ning Liu, Kevin McDonald, Aran Paulus, Anton Posch
  • Patent number: 9597352
    Abstract: In the present invention, Applicants demonstrate the effect of a biomembrane sealing agent on the development of chronic pain following tissue injury as well as acute pain in a model of acute inflammation. Applicants demonstrate the ability of this class of agents referred to as “biomembrane sealing agents” to reduce the severity of hyperalgesia and allodynia following mechanical insult to the nervous system as well as their ability to reduce acute pain in a model of acute inflammation. Applicant describes the use of injectable or depot formulations of biomembrane sealing agent(s) for prophylactic treatment such as they could be administered after the insult (i.e. post-injury or post-surgery) but before the onset of acute or chronic pain. Alternatively, biomembrane sealing agents could be used to reduce the severity of acute or chronic pain after onset.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: March 21, 2017
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Josee Roy, Jeffrey C. Marx
  • Patent number: 9594074
    Abstract: Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques. The present invention provides methods for detecting the presence or amount of C peptide in a sample by mass spectrometry.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: March 14, 2017
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Zhaohui Chen
  • Patent number: 9574236
    Abstract: Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.
    Type: Grant
    Filed: January 6, 2015
    Date of Patent: February 21, 2017
    Assignee: Agilent Technologies, Inc.
    Inventors: Brian Phillip Smart, Robert A. Ach
  • Patent number: 9567626
    Abstract: A monolithic device comprises a substrate. An array of sensing elements is coupled to the substrate, and each sensing element includes a magnetoresistive sensor. An analog electric drive generator is coupled to the substrate, and the electric drive generator produces a sensor-biasing signal that biases the magnetoresistive sensors of the array. An analog multiplexer is coupled to the substrate, and outputs of the array are coupled to inputs of the multiplexer. An analog signal conditioning circuit is coupled to the substrate, wherein at least one output of the multiplexer is coupled to at least one input of the signal conditioning circuit. The monolithic device is fabricated using both complementary metal-oxide semiconductor (i.e., CMOS) technology and thin film technology. For example, the electric drive generator, the multiplexer, and the signal conditioning circuit may be fabricated with CMOS technology, while the magnetoresistive sensors of the array are fabricated with thin film technology.
    Type: Grant
    Filed: July 3, 2014
    Date of Patent: February 14, 2017
    Assignee: MAGNOMICS, S.A.
    Inventors: Filipe Arroyo Cardoso, Tiago Miguel Lopes Marta da Costa, José António Henriques Germano, Moisés Simões Piedade
  • Patent number: 9547013
    Abstract: This invention provides methods and biomarkers for diagnosing autism by identifying cellular metabolites differentially produced in autistic patient samples versus non-autistic controls. Methods for identifying a unique profile of metabolites present of secreted in brain tissue, cerebrospinal fluid, plasma, or biofluids of autistic samples are described herein. The individual metabolites or a pattern of secreted metabolites provide metabolic signatures of autism, which can be used to provide a diagnosis thereof.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: January 17, 2017
    Assignees: Wisconsin Alumni Research Foundation, Stemina Biomarker Discovery, Inc.
    Inventors: Gabriela Gebrin Cezar, Alan M. Smith
  • Patent number: 9448240
    Abstract: A thiol probe for a protein, which promotes ionization in proteomic analysis using mass spectrometry and can be used even for a protein that has a high degree of hydrophobicity and quickly turns over, and is represented by the following formula (I): In formula (I), R1 represents a linker group, and R2 represents a substituted ammonium group or a substituted amino group. A mass spectrometry method for a protein, includes the steps of obtaining a modified protein by reacting the thiol probe with a protein to be subjected to mass spectrometry and subjecting the modified protein to mass spectrometry.
    Type: Grant
    Filed: August 17, 2012
    Date of Patent: September 20, 2016
    Assignee: SHIMADZU CORPORATION
    Inventors: Takashi Shimada, Taka-Aki Sato, Koichi Tanaka
  • Patent number: 9448147
    Abstract: A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: September 20, 2016
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventors: Ian Christopher Parsons, Ting Zheng, Nur Sibel Gunay, Carlos J. Bosques
  • Patent number: 9405884
    Abstract: The present invention relates to methods for analyzing protein samples, e.g., monoclonal antibodies, via the SITRS technique, as well as methods and systems that facilitate the processing of data generated by the SITRS technique.
    Type: Grant
    Filed: December 20, 2011
    Date of Patent: August 2, 2016
    Assignee: AbbVie Inc.
    Inventors: David H. Lee, Anton V. Manuilov
  • Patent number: 9394511
    Abstract: A disposable rapid cell sorter comprises of microfluidic chip with electrodes and sorts biological cells of interest through magnetic field and electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.
    Type: Grant
    Filed: December 1, 2011
    Date of Patent: July 19, 2016
    Inventor: Wenbin Jiang
  • Patent number: 9329201
    Abstract: Methods, devices, and systems for forming atomically precise structures are provided. In some embodiments, the methods, devices, and systems of the present disclosure utilize a scanning tunneling microscope (STM) to remove portions of a monolayer of atoms or molecules from a crystalline surface to form atomically precise structures. The STM is utilized to both image the sample and remove the desired portions of the monolayer of atoms or molecules. In some instances, the lattice structure of the crystalline surface is utilized as a coordinate system by a control system of the STM to facilitate the automated removal of specific atoms or molecules from the crystalline surface.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: May 3, 2016
    Assignee: Zyvex Labs LLC
    Inventors: John Neal Randall, James R. Von Ehr, James H. G. Owen, Joshua B. Ballard, Ehud Fuchs
  • Patent number: 9296785
    Abstract: A targeting peptide that specifically binds to an IL13 receptor (e.g., wherein said targeting peptide is not an IL13 fragment) is described. The targeting peptide is optionally conjugated to at least one effector molecule. In some embodiments, the peptide specifically binds to the IL13R?2 protein.
    Type: Grant
    Filed: April 16, 2010
    Date of Patent: March 29, 2016
    Assignee: Wake Forest University Health Sciences
    Inventors: Waldemar Debinski, Hetal Pandya, Denise Gibo
  • Patent number: 9274124
    Abstract: Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.
    Type: Grant
    Filed: March 15, 2011
    Date of Patent: March 1, 2016
    Assignee: ANDERSON FORSCHUNG GROUP, INC.
    Inventor: Norman Leigh Anderson
  • Patent number: 9261506
    Abstract: The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins that are particularly advantageous for quantifying the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded.
    Type: Grant
    Filed: November 17, 2014
    Date of Patent: February 16, 2016
    Assignee: EXPRESSION PATHOLOGY, INC.
    Inventors: David B. Krizman, Wei-Li Liao, Sheeno Thyparambil, Todd Hembrough
  • Patent number: 9217743
    Abstract: With an aim to provide means for developing a compound devoid of teratogenicity but retaining beneficial actions, a screening method for a non-teratogenic substance comprising bringing a test substance into contact with cereblon or a fragment of cereblon, evaluating the bindability of the test substance with cereblon or the fragment of cereblon, and selecting a test substance that does not bind to cereblon or the fragment of cereblon or a test substance exhibiting lower bindability with cereblon or the fragment of cereblon than does thalidomide is provided.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: December 22, 2015
    Assignees: Tokyo Institute of Technology, Fujimoto Pharmaceutical Corporation
    Inventors: Hiroshi Handa, Hideki Ando, Takumi Itoh, Kentaro Hotta
  • Patent number: 9201012
    Abstract: Embodiments of the present disclosure provide methods for analyte sensors including methods for producing and using the analyte sensors, methods of detecting and/or measuring analyte activity, methods for characterizing analyte cellular activity, methods of detecting pH change in a system, method of controlling the concentration of an analyte in a system, fusion proteins, polynucleotides, and vectors corresponding to the analyte sensors, kits, and the like.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: December 1, 2015
    Assignee: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Jenny Jie Yang, Jin Zou, April L. Ellis Mitchem, Yiming Ye, Angela Holder, Yun Huang, Wei Yang, Michael Kirberger
  • Patent number: 9196465
    Abstract: A method for detecting hepcidin. Having a sample liquid in contact with a nanochip having a specific surface coating structure of silicon oxide, so that hepcidin is enriched with specificity; eluting the nanochip with an eluent; by performing mass spectrometric detection on the elution product, determining the hepcidin content in the elution product. The enrichment method substantially enhances the sensitivity and accuracy of mass spectrometric detection. A kit for detecting hepcidin comprising a sample diluent and a nanochip, the sample diluent comprising water, trifluoroacetic acid, and acetonitrile.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: November 24, 2015
    Assignee: National Center for Nanoscience and Technology
    Inventors: Guangjun Nie, Tony Hu, Jia Fan, Yuliang Zhao, Mauro Ferrari
  • Patent number: 9128168
    Abstract: Methods and kits of the invention are useful for calculating 24-hour excretion of sodium without the need to collect urine for 24 hours.
    Type: Grant
    Filed: December 12, 2008
    Date of Patent: September 8, 2015
    Assignee: Cornell University
    Inventors: Samuel J. Mann, Linda M. Gerber
  • Patent number: 9095547
    Abstract: Glatiramer acetate containing compositions and drug products can be manufactured using methods that include a step of measuring one or both of the alpha helical content of the glatiramer acetate and the random coil content of the glatiramer acetate. These measurements can be compared to particular values or ranges for one or both of alpha helical and random coil content. The results of the comparison can be used in determining whether a given batch of glatiramer acetate should be used to manufacture a glatiramer acetate-containing drug product. The measurement of alpha helical content of the glatiramer acetate and the random coil content can be carried out using various methods, including circular dichroism.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: August 4, 2015
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventor: Ramamurthi Sridharan
  • Patent number: 9091698
    Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: July 28, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Song Shi, Richard L. Moore, James Garrett