Abstract: The determination of fructosamine levels in at least part of a blood sample for use in detecting diabetes in patients or deciding treatment levels is effected by controlling the temperature and pH of the sample, adding a coloring agent such as a nitro-blue tetrazolium and after a first delay in time taking a first color measurement at a predetermined wavelength and after a second delay in time taking a second color measurement at the predetermined wavelength, then comparing any resultant change between the first and second color measurements with those of standard solutions, the selected timing delays, wavelength, coloring agent and pH conditions being such that any change of color in the coloring agent between the first and second color measurements is caused predominantly by the glucose in the sample that is reacted with an amine group of protein and has undergone a molecular rearrangement to form fructosamine and not materially by any non-specific reducing substance which may be present in the sample.

Abstract: A test system having an extended range of measurement and an appropriate procedure for the determination of NAD(P)H or of substrates or enzymes which react to form or consume NAD(P)H in fluids is provided. The test system contains, at one and the same time, several substances acting independently of one another as electron acceptors with respect to NAD(P)H and having different electrochemical potentials. Addition of the test system to the sample solution gives rise to different end products which can be analytically differentiated and which are evaluated visually or by other techniques of measurement.

Abstract: Fecal occult blood tests of enhanced sensitivity and specificity are provided by employing (1) a developer composition comprising a solution of a hydroperoxide in a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins such as dimethyl sulfoxide and/or (2) a solid test matrix comprising a solid support impregnated with a leuco dye and a hemoprotein solubilizing agent. Test results may be further improved by incorporating plant peroxidase inhibitors, iron/copper chelating agents and buffers in the developer and/or test matrix.

Abstract: A stabilized indicator powder for use in assays to detect the presence of peroxidase or other peroxidatively active substances, wherein the indicator is stabilized by being combined with a water soluble polymer. As a dry powder, the indicator retains its reactivity for at least several months. The powder readily dissolves in an aqueous medium and as a solution retains its reactivity for a period of weeks, even in the presence of peroxide.

Abstract: A composition, test means (and device) and method for determining peroxidatively active substances in a test sample are disclosed. The composition, test means (and device) and method are rendered resistant to the adverse affects of ascorbate which may be present in the sample by the inclusion in the composition of a metal chelate which is polycarboxyalkylamine derivative having the formula: ##STR1## where: (a) R.sub.1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R.sub.2, R.sub.3, R.sub.x and R.sub.y, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R.sub.1, R.sub.2, R.sub.3, R.sub.x or R.sub.y are alkyl carboxylic acid radicals so defined;(b) R.sub.p and R.sub.

Abstract: A test kit for preparing a chemical test pad used in testing for the presence of occult blood in a specimen. The test kit is a foil package enclosing an absorbant pad bearing a peroxygen agent and a solution of guaiac in alcohol in a separate packet or compartment from the absorbant pad. The separate packet or compartment being rupturable by applying hand pressure to the outside of the foil package to mix the peroxygen agent powder on the test pad and the guaiac solution prior to removing the absorbant pad from the foil package. The method relates to performing a medical test by activating a test pad bearing one reagent by applying another reagent thereto while remaining wholly within a protective foil package. According to the method a test pad containing the peroxygen agent is wetted with a solution of guaiac in alcohol prior to removal from the protective foil package and exposure to a specimen of fecal material and water in a test for occult blood.

Abstract: Compounds of the general formula ##STR1## wherein R represents an alkyl group of 1 to 3 carbon atoms or the group N(R.sub.2).sub.2, in which R.sub.2 represents a hydrogen atom, an alkyl group of 1 to 3 carbon atoms, or an acyl group of 1 to 3 carbon atoms, and R.sub.1 has the same meaning as R or is a hydrogen atom, are new and are suitable as chromogens for measuring H.sub.2 O.sub.2 formation in enzymatic reactions.

Abstract: A test composition, device and method for determining a perioxidatively active substance in a test sample, as well as a method for preparing and using the device, are disclosed. The composition comprises an organic hydroperoxide, a benzidine indicator capable of providing a detectable response in the presence of the organic hydroperoxide and a peroxidatively active substance, and, as a stabilizing agent, an aniline chosen from pheny-1-naphthylamine, N,N-dimethylaniline and mixtures thereof. The device comprises a carrier matrix incorporated with the composition, and the method for using the device comprises contacting a test sample with the device and observing a detectable response therein. A method for preparing the device comprises preparing two or more solutions of the ingredients of the composition, wetting the carrier matrix sequentially therewith and drying the matrix after each wetting.

Abstract: A test pad having a water activated reagent and a water activated peroxygen compound which undergo a chromogen reaction in an aqueous solution including hemoglobin. The reagent is potassium guaiacolsulfonate, a guaiac substitute material. The peroxygen compound is a monopersulfonate compound comprised of two moles of potassium monopersulfonate, one mole of potassium hydrogen sulfate and one mole of potassium sulfate. The test pad features a test area containing the above materials in powdered form and may also include controls for checking the results of the test. A method for using the test pad is also disclosed.

Abstract: A method for the determination of 5-aminosalicylic acid, which comprises measuring the color developed in an alkaline medium by an oxidative condensation product formed by the condensation of an o-alkylphenol represented by the general formula ##STR1## (wherein R.sub.1 represents an ethyl, isopropyl or sec-butyl group) with 5-aminosalicylic acid of the formula ##STR2## in the presence of a peroxide.

Abstract: A tamper-resistant package containing in an anerobic environment a leuco dye free of reducing agent which dye becomes colored upon reaction with oxygen.

Abstract: A process for rapidly differentiating between gram-positive and gram-negative microorganisms by mixing an anionic surfactant to inhibit the reduction capability of the gram-positive microorganisms, an electron transfer agent and a compound capable of being reduced to a detectable species by both gram-positive and gram-negative microorganisms in the absence of an anionic surfactant.

Abstract: Improved catalyzed substrates for use in developing characteristic colors or fluorescence in the presence of peroxidase enzymes (e.g., horseradish peroxidase) are disclosed which include as a rate accelerator a substituted phenol such as a p-halogenated phenol. The complete system typically includes a peroxide type oxidizing agent (e.g., hydrogen peroxide), a chromogenic or flurogenic compound (e.g., ABTS), a buffer and the accelerator compound. Advantageously, the accelerator should provide at least about 50 percent rate enhancement for the substrate, as compared with an otherwise identical, accelerator-free substrate reacted under the same conditions; however, the most preferred accelerator, p-iodophenol, gives enhancements on the order to 1,000 percent. The invention is particularly useful in so-called ELISA determinations which involve an enzyme-linked moiety, and permit detection at very low concentration levels unobtainable with conventional colorimetric substrates.

Abstract: Composition for the detection of(a) hydrogen peroxide or hydrogen peroxide forming substrates or(b) peroxidases or peroxidatively reacting substances which composition comprises a substrate capable of coupling and an oxidizable substance known to undergo the Trinder reaction, and(a) a peroxidase or(b) hydrogen peroxidewherein the substrate capable of coupling is a compound of the formula ##STR1## in which n and m are individually selected from whole numbers of 1 to 4,X and Y, which can be the same or different represent a valence bond or a phenylene radical,R.sub.1 and R.sub.2 are individually selected from carboxyl or sulfonic acid groups and one of R.sub.1 and R.sub.2 can also be hydrogen or lower alkyl, andR.sub.3 and R.sub.4 are individually selected from hydrogen and alkyl radicals of up to 6 carbon atoms;and/or a salt of such compound.

Abstract: What are disclosed are a method for detecting substances having a peroxidative effect using a substituted or condensed pyridine, and a diagnostic agent containing a pyridine of this kind.

Abstract: A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.

Abstract: An indicator (10) for detecting contact with a naturally occurring polyhydroxyaromatic skin irritant or toxin like that found in poison ivy and the like comprises a carrier (12) treated with a reactant, such as ferric nitrate which reacts with the toxin and produces a distinct color change indicative of such contact.

Abstract: A composition, test device and method for detecting the presence and/or determining concentration of glucose in a liquid test sample are disclosed. The composition is capable of determining glucose concentration over a broad range, e.g. 0 to about 5,000 milligrams (mg) of glucose per deciliter (dl) of test sample. Moreover, the composition is resistant to interference from the presence of ascorbate in the sample. The composition comprises glucose oxidase, peroxidase and, as an indicator capable of producing a detectable response in the presence of peroxidase and H.sub.2 O.sub.2, a mixture of two compounds. One has the structure ##STR1## and the other has the structure ##STR2## in which R, same of different, is H or lower alkyl, R' is aryl and R" is lower alkyl.

Abstract: A test composition, device and method for determining a peroxidatively active substance in a test sample, as well as a method for preparing the device, are disclosed. The composition comprises an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of the organic hydroperoxide and peroxidatively active substance, wherein the organic hydroperoxide is a substituted cumene hydroperoxide having the formula: ##STR1## in which any one of the X substituents is lower alkyl of from 1 to 6 carbon atoms, Cl, Br, I, NO.sub.2 or carboxyl; or any two of the X substituents, same or different, are lower alkyl of from 1 to 6 carbon atoms, Cl, Br, I, NO.sub.2 or carboxyl. The device comprises a carrier matrix incorporated with the composition, and the method comprises contacting a test sample with the device and observing a detectable response in the device.

Abstract: Means and method for the detection of hydrogen peroxide or of hydrogen peroxide-forming substrates, consisting of 4-aminoantipyrine and an aromatic amine or phenol and a neutral to weakly acid buffer as well as other additives and reagents which form H.sub.2 O.sub.2 with the substrate and which are known for use in Trinder's reaction, the 4-aminoantipyrine, in the immiscible state before use, being separate from the acid buffer and from the other reagents if any, and being mixed with a small amount of an alkaline buffer having a pH of 8.5 to 14.0, the two compositions being combined, in a quantity ratio to one another that is suitable for the detection test, in a single package or in a single-use unit.

Abstract: In a multilayer analysis film for detecting hydrogen peroxide by dry process which comprises a support and a reagent layer having contained therein a color indicator composition comprising peroxidase and a substance capable of causing a optically detectable change in the presence of hydrogen peroxide and peroxidase, the color indicator composition is characterized by further containing specific pyrogallol derivatives. Such pyrogallol derivatives improve stability during storage of such a multilayer analysis film.

Abstract: The invention pertains to a method for the study of the behaviour or detection of cells, organites or cellular fractions, possibly present in a medium.It comprises providing said medium with the essential nutrients to promote the activity of the said cells, organites or cellular fractions, if any, as well as with an "electron transporter" such as lipoic acid which is liable of forming a redox system and of intervening in one of the metabolic routes involved by the above said activity, particularly growth of cells.The method of the invention then further comprises measuring and detecting a possible modification of the relative proportions of the oxidized and reduced form of the "electron transporter". The time required for such modification to possibly take place, as well as other parameters of the reaction can then be correlated to the presence in the medium concerned of a cellular activity or analogous.

Abstract: The invention relates to diagnostic agents for the rapid diagnostic detection of blood in excretions and body fluids, consisting essentially of a solid support which is charged with a chromogenic substance as indicator and impregnated with an acrylic and/or methacrylic resin.

Abstract: In a test piece for the detection of occult blood, obtained by impregnating a substrate with an organic peroxide, a chromogen, a buffer, and a stabilizer, at least one compound selected from the group of compounds represented by the general formula I: ##STR1## wherein, m denotes an integer of the value of 1 to 5, R.sup.1 denotes one member selected from the group consisting of methyl group, lower alkoxy group, halogen atoms, and hydrogen atom, and X denotes one group selected from the group consisting of ##STR2## where, R.sup.2 and R.sup.3 each denote an alkyl group having 1 or 2 carbon atoms, ##STR3## where, m, R.sup.1, R.sup.2, and R.sup.3 are as defined above, R.sup.4 denotes an alkylene group having 1 to 6 carbon atoms, and n is 0 or 1, ##STR4## where, m, R.sup.1, R.sup.4 are as defined above, and ##STR5## where, R.sup.2 and R.sup.3 are as defined above.

Abstract: An improved composition, device and method, whereby enhanced sensitivity is achieved in detecting test sample constituents such as glucose and blood. The improvement resides in the use of a broad range of compounds as enhancers for benzidine-type indicators. The enhancer tends to stabilize the blue colorform of such indicators.