Saccharide (e.g., Dna, Etc.) Patents (Class 436/94)
  • Patent number: 10692001
    Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for processing cell images using neural networks. One of the methods includes obtaining data comprising an input image of one or more biological cells illuminated with an optical microscopy technique; processing the data using a stained cell neural network; and processing the one or more stained cell images using a cell characteristic neural network, wherein the cell characteristic neural network has been configured through training to receive the one or more stained cell images and to process the one or more stained cell images to generate a cell characteristic output that characterizes features of the biological cells that are stained in the one or more stained cell images.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: June 23, 2020
    Assignee: Google LLC
    Inventors: Philip Charles Nelson, Eric Martin Christiansen, Marc Berndl, Michael Frumkin
  • Patent number: 10633713
    Abstract: Various embodiments are directed to applications (e.g., classification of biological samples) of the analysis of the count, the fragmentation patterns, and size of cell-free nucleic acids, e.g., plasma DNA and serum DNA, including nucleic acids from pathogens, such as viruses. Embodiments of one application can determine if a subject has a particular condition. For example, a method of present disclosure can determine if a subject has cancer or a tumor, or other pathology. Embodiments of another application can be used to assess the stage of a condition, or the progression of a condition over time. For example, a method of the present disclosure may be used to determine a stage of cancer in a subject, or the progression of cancer in a subject over time (e.g., using samples obtained from a subject at different times).
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: April 28, 2020
    Assignees: The Chinese University of Hong Kong, GRAIL, Inc.
    Inventors: Yuk-Ming Dennis Lo, Rossa Wai Kwun Chiu, Kwan Chee Chan, Peiyong Jiang, Wai Kei Lam
  • Patent number: 10627406
    Abstract: A method for detecting glutathione includes mixing a sample including glutathione, 9-bromomethyl acridine (Br-MA) and a derivatization solvent to form a reaction solution. A derivative reaction occurs between Br-MA and glutathione to obtain a derivatization solution including a glutathione derivative with a thiol group being substituted with a tag. Excess Br-MA is removed by adding an interference removing solvent into the derivatization solution, followed by vortexing and centrifugation to obtain an aqueous layer solution. The aqueous layer solution is used as an analytic solution, and the glutathione derivative in the analytic solution is detected to obtain a glutathione value. The present invention also provides a kit for detecting glutathione which is adapted to carry out the method for detecting glutathione.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: April 21, 2020
    Assignee: KAOHSIUNG MEDICAL UNIVERSITY
    Inventors: Chia-Hsien Feng, Chen-Wen Chen
  • Patent number: 10589276
    Abstract: Disclosed herein is a multi-functional microfluidics device capable of isolation of nucleic acids, purification of nucleic acids, performance of Polymerase Chain Reactions (PCRs), in situ hybridization of nucleic acids, fluorescent signal detections and the like. The apparatus comprises an integration of a plurality of reagent chambers, a sample input, a reaction chamber, a detection chamber, and a waste chamber; wherein the plurality of reagent chambers is fluidly connected to the reaction chamber, and is configured to store a plurality of reagents; wherein the sample input is fluidly connected to the reaction chamber, and is configured to receive a sample; wherein the waste chamber is fluidly connect to the reaction chamber and is configured to receive a reaction waste from the reaction chamber.
    Type: Grant
    Filed: August 30, 2018
    Date of Patent: March 17, 2020
    Assignee: SHENZHEN GENORIVISION TECHNOLOGY CO., LTD.
    Inventors: Rui Ding, Peiyan Cao
  • Patent number: 10564119
    Abstract: A DNA sequencing device includes a first layer having a nanochannel formed therein, and a pair of electrodes arranged vertically relative to each other and spaced apart to define an electrode gap. The electrode gap is exposed in the nanochannel, and the electrode gap is in the range of about 0.3 nm to about 2 nm.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: February 18, 2020
    Assignee: SEAGATE TECHNOLOGY LLC
    Inventors: David S. Kuo, ShuaiGang Xiao, Kim Yang Lee, Xiaomin Yang, Koichi Wago, Thomas Young Chang
  • Patent number: 10538800
    Abstract: A TFT biosensor includes a gate electrode (silicon substrate), a reference electrode, and enzyme that is fixed to an insulating substrate spatially separated from the gate electrode and the reference electrode. A pH variation in the vicinity of an ion-sensitive insulating film is induced by a reaction between the enzyme and a sensing object material. The TFT biosensor can detect a concentration of the sensing object material with high sensitivity by detecting the pH variation as a threshold voltage shift of characteristics of a gate-source voltage to a source-drain current.
    Type: Grant
    Filed: July 9, 2019
    Date of Patent: January 21, 2020
    Assignee: TIANMA MICROELECTRONICS CO., LTD.
    Inventors: Kazushige Takechi, Shinnosuke Iwamatsu, Yutaka Abe, Toru Yahagi, Shunsuke Konno, Mutsuto Katoh
  • Patent number: 10436742
    Abstract: In one implementation, a method for manufacturing a chemical detection device is described. The method includes forming a chemical sensor having a sensing surface. A dielectric material is deposited on the sensing surface. A first etch process is performed to partially etch the dielectric material to define an opening over the sensing surface and leave remaining dielectric material on the sensing surface. An etch protect material is formed on a sidewall of the opening. A second etch process is then performed to selectively etch the remaining dielectric material using the etch protect material as an etch mask, thereby exposing the sensing surface.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: October 8, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shifeng Li, James Bustillo
  • Patent number: 10381107
    Abstract: A nucleic acid sequencer includes: an atomically thin membrane; a solid electrode spaced apart from the atomically thin membrane and arranged in a capacitive configuration with the atomically thin membrane; a spacer member; a complementary base covalently disposed on the atomically thin membrane and arranged to base pair with a nitrogenous base of the single stranded nucleic acid; a power source in electrical communication with the solid electrode and that provides electrical power to the solid electrode; and a resistor in electrical communication with the power source and that receives electric current from the power source and that also is in electrical communication with the atomically thin membrane such that an amount of the electric current changes in response to a change in the selected distance between the atomically thin membrane and the solid electrode.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: August 13, 2019
    Assignee: GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE
    Inventor: Alex Smolyanitsky
  • Patent number: 10336713
    Abstract: Triazole-based molecules, methods of making and using the same are provided. Triazole-based molecules may be used as reading molecules and incorporated into or operatively-linked with electrodes, for example, and used in recognition tunneling systems to identify individual and/or sequences of molecules (e.g., DNA bases, carbohydrates, proteins, peptides, and/or amino-acids).
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: July 2, 2019
    Assignee: Arizona Board of Regents, Acting for and on behalf of, Arizona State University
    Inventors: Peiming Zhang, Stuart Lindsay, Sovan Biswas, Suman Sen
  • Patent number: 10329612
    Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: June 25, 2019
    Assignee: Agena Bioscience, Inc.
    Inventor: Thomas Becker
  • Patent number: 10274478
    Abstract: Provided are suspended solid-state membranes on glass chips with improved capacitance. The membranes include a first cavity formed in the thickness of the glass membrane, the first cavity having a width that increases along the direction extending from the first surface of the membrane to the second surface of the membrane, a first membrane surmounting at least a portion of the first surface of the glass membrane, the first membrane having a pore formed therethrough, and the pore of the first membrane being in fluid communication with the first cavity of the glass membrane. Also provided are related methods of fabricating the disclosed chips and of using the disclosed chips for macromolecular analysis.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: April 30, 2019
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Adrian Balan, Marija Drndic
  • Patent number: 10260154
    Abstract: The invention discloses a method for the synthesis of monodispersed luminescent quantum dots of transition metal dichalcogenides (TMDC), single- or few-layered, using a single-step electrochemical exfoliation that involves dilute ionic liquid and water. The method disclosed helps to obtain nanoclusters of TMDC of desired size including small sizes ranging up to 6 nm, by varying the concentration of the electrolyte and the applied DC voltage. The invention further discloses a method by which mono- or few-layered luminescent transition metal dichalcogenides can be directly deposited onto conducting substrates in a uniform manner. The monodispersed single- or few-layered luminescent TMDC and electro-deposited substrates exhibit improved electronic conductivity and new active sites, making them suitable as high-performance electrocatalysts in hydrogen evolution reactions in solar water-splitting applications and also as electrodes for solar cell applications.
    Type: Grant
    Filed: August 1, 2016
    Date of Patent: April 16, 2019
    Inventors: Shaijumon Mancheri Manikoth, Dijo Damien, Deepesh Gopalakrishnan
  • Patent number: 10245310
    Abstract: The invention provides a conjugate comprising an antigen and a carrier molecule, wherein the carrier molecule comprises a BP-2a antigen and a spb1 antigen. BP-2a and spb1 are Streptococcus agalactiae antigens. The conjugate may be used in a method for raising an immune response in a mammal, the method comprising administering the conjugate to the mammal. Also provided are pharmaceutical compositions, particularly vaccines, comprising the conjugate.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: April 2, 2019
    Assignee: GlaxoSmithKline Biologicals SA
    Inventors: Francesco Berti, Roberta Cozzi, Domenico Maione, Cira Daniela Rinaudo, Immaculada Margarit Y Ros, Guido Grandi
  • Patent number: 10088477
    Abstract: Described here are a device and a method for detecting the presence of a biomarker using the device, wherein the device comprises (a) a substrate comprising a plurality of electrodes; (b) a plurality of nanowire field-effect transistor sensors integrated or assembled on the substrate and connected to the electrodes; and (c) a microfluidic component disposed on the substrate and adapted to communicate fluidically with the nwFET sensors.
    Type: Grant
    Filed: July 30, 2013
    Date of Patent: October 2, 2018
    Assignee: The Regents of the University of California
    Inventor: Chi On Chui
  • Patent number: 10058868
    Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.
    Type: Grant
    Filed: December 12, 2014
    Date of Patent: August 28, 2018
    Assignee: BioFire Diagnostics, LLC
    Inventors: Kirk M. Ririe, Michael R. Newswander, Randy P. Rasmussen, Mark Aaron Poritz, Stewart Benjamin Smith, David E. Jones, Gary Clark Kessler
  • Patent number: 10041884
    Abstract: Provided is a nucleic acid analyzer, which does not require manual processes by a highly trained operator such as a researcher and is easy to use, small-sized, capable of accepting multiple samples, and performs speedy analysis, and a nucleic acid analysis method using the analyzer. The analyzer and method perform detection in a plurality of exposure times, provide a program for determining a threshold for signal detection, and determine whether a faint signal peak is a false signal peak.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: August 7, 2018
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Michiru Fujioka, Motohiro Yamazaki, Toru Yokoyama
  • Patent number: 10018640
    Abstract: Aspects of the present disclosure include methods and systems for assaying a sample for an analyte. Methods according to certain embodiments include illuminating a sample with a slit-shaped beam of light, detecting light transmitted through the sample, determining absorbance of the transmitted light at one or more wavelengths and calculating concentration of the analyte based on the absorbance to assay the sample for the analyte. Systems for practicing the subject methods are also described.
    Type: Grant
    Filed: November 10, 2014
    Date of Patent: July 10, 2018
    Assignee: Becton, Dickinson and Company
    Inventors: Scott Bornheimer, Edward Goldberg
  • Patent number: 9795687
    Abstract: To study RNA function using small molecules, we designed bioactive, modularly assembled small molecules that target the noncoding expanded RNA repeat that causes myotonic dystrophy type 1 (Dm1), r(CUG)exp. Different modular assembly scaffolds were investigated including polyamines, alpha-peptides, beta-peptides, and peptide tertiary amides (PT As). Based on activity as assessed by improvement of DM1 -associated defects, stability against proteases, cellular permeability, and toxicity, we discovered that constrained backbones, namely PT As, are optimal.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: October 24, 2017
    Assignee: The Scripps Research Institute
    Inventors: Matthew D. Disney, Suzanne Rzuczek
  • Patent number: 9782692
    Abstract: A fluid supply system configured for supplying fluids includes a fluid packet supply unit configured for controlling supply of a sequence of fluid packets. The fluid packets include a packet of first fluid and a packet of second fluid, wherein the first fluid and the second fluid are media being prone to a phase separation upon direct interaction between the packet of first fluid and the packet of second fluid. The fluid supply system further includes a phase separation inhibiting unit configured for inhibiting phase separation by inserting an intermediate fluid packet between the packet of first fluid and the packet of second fluid.
    Type: Grant
    Filed: April 25, 2012
    Date of Patent: October 10, 2017
    Assignee: Agilent Technologies, Inc.
    Inventors: Klaus Witt, Konstantin Shoykhet
  • Patent number: 9753029
    Abstract: The present invention provides a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a liquid sample by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD4+, CD8+ cells, malaria-infected red blood cells, cancer cells, cancer biomarkers such as prostate specific antigen and other cancer biomarkers, viruses, bacteria, and other pathogenic agents, with the sensitivity, specificity and accuracy of bench-top laboratory-based assays.
    Type: Grant
    Filed: January 6, 2014
    Date of Patent: September 5, 2017
    Assignee: Carnegie Mellon University
    Inventors: Alberto Gandini, James F. Antaki, Byron Wang Chuan, Joie N. Marhefka
  • Patent number: 9725753
    Abstract: Provided is a device that, on the basis of a measurement result of a current that has a low value and a wide distribution, identifies the composition of biological molecules passing through a nanoparticle path. This biomolecule information analysis device obtains a current value by applying an electrical field to biomolecules passing through a gap between a first electrode and a second electrode, and identifies the structure of the biomolecules by integrating the current value and making a comparison with a reference value (see FIG. 1).
    Type: Grant
    Filed: May 31, 2011
    Date of Patent: August 8, 2017
    Assignee: Hitachi, Ltd.
    Inventors: Kazuo Ono, Tatsuo Nakagawa, Yoshimitsu Yanagawa, Takayuki Kawahara, Akira Kotabe, Riichiro Takemura
  • Patent number: 9651518
    Abstract: The present invention provides a nano-fluidic field effective device. The device includes a channel having a first side and a second side, a first set of electrodes adjacent to the first side, a second set of electrodes adjacent to the second side, a control unit for applying electric potentials to the electrodes and a fluid within the channel containing a charge molecule. The first set of electrodes is disposed such that application of electric potentials produces a spatially varying electric field that confines a charged molecule within a predetermined area of said channel. The second set of electrodes is disposed such that application of electric potentials relative to the electric potentials applied to the first set of electrodes creates an electric field that confines the charged molecule to an area away from the second side of the channel.
    Type: Grant
    Filed: November 17, 2014
    Date of Patent: May 16, 2017
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Glenn J. Martyna, Dennis M. Newns, Hongbo Peng, Stanislav Polonsky, Stephen Rossnagel, Gustavo Stolovitzky
  • Patent number: 9513277
    Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: December 6, 2016
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Hongbo Peng, Stephen M. Rossnagel, Ajay K. Royyuru, Gustavo A. Stolovitzky, Philip S. Waggoner
  • Patent number: 9482648
    Abstract: A method for detecting and quantifying galacto-oligosaccharide in a sample containing galacto-oligosaccharide and dextrin is characterized in that the sample is caused to react with a derivatizing reagent to derivatize the dextrin and galacto-oligosaccharide in the sample, after which the galacto-oligosaccharide component in the sample is separated by high-performance liquid chromatography using a C30 reverse-phase chromatography column, thereby accurately detecting and quantifying galacto-oligosaccharide with ease and at low cost.
    Type: Grant
    Filed: September 26, 2014
    Date of Patent: November 1, 2016
    Assignee: KABUSHIKI KAISHA YAKULT HONSHA
    Inventors: Harumi Mizukoshi, Kazumasa Kimura
  • Patent number: 9476836
    Abstract: The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: October 25, 2016
    Assignee: KANEKA CORPORATION
    Inventors: Shigehiko Miyamoto, Jun Tomono, Koji Takahashi, Sotaro Sano, Takaaki Jikihara
  • Patent number: 9452927
    Abstract: A method and device for the rapid detection of biomolecules (320) diffusing in a nanometer-confined slit (204) is claimed. In particular, the present invention relates to a novel concept of fluidic side apertures (205) that facilitates the filling of the device, the surface coating with biomolecules and that measures the affinity between fluorescently labeled biomolecules in aqueous solution with other biomolecules immobilized on surfaces.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: September 27, 2016
    Assignee: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
    Inventors: Nicolas Durand, Yannick Fournier, Theo Lasser, Iwan Märki
  • Patent number: 9441053
    Abstract: The present invention describes an analytical method for detecting and quantitating poly-sulfated oligosaccharides, including Fondaparinux sodium, using hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) coupled with a charged aerosol detector (CAD) or a mass spectrometer (MS). This analytical method provides in-process control in a total synthesis of highly sulfated oligosaccharides by separation, quantification and mass identification. Systems and conditions utilizing such methods are also provided.
    Type: Grant
    Filed: July 11, 2013
    Date of Patent: September 13, 2016
    Assignee: ScinoPharm Taiwan, Ltd.
    Inventors: ChungYao Wang, Imin Huang, ChiaYen Wu, YungTe Chiang, Helen Chao
  • Patent number: 9439909
    Abstract: The invention provides for the use of radiation sensitizing agents in combination with radiation for the treatment of neoplasia, methods for the identification of genotype-specific radiation sensitizing agents, and methods of identifying patients who could benefit from therapy with a genotype-specific radiation sensitizing agent.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: September 13, 2016
    Assignees: The Broad Institute, Inc., Dana-Farher Cancer Institute, Inc., President and Fellows of Harvard College
    Inventors: Mohamed Abazeed, Matthew Meyerson, Drew Adams
  • Patent number: 9391285
    Abstract: The present invention relates to a method and a device for providing a current of spin-polarized electrons. More particularly, the present invention is suited for use in spin electronics or detection of spin-polarized electrons.
    Type: Grant
    Filed: February 3, 2012
    Date of Patent: July 12, 2016
    Assignees: Westfälische Wilhelms Universität Münster, Yeda Research and Development Co. Ltd. at the Weizmann Institute of Science
    Inventors: Benjamin Göhler, Volker Hamelbeck, G. Friedrich Hanne, Helmut Zacharias, Ron Naaman, Tal Zvi Markus
  • Patent number: 9375711
    Abstract: “Click-assembly” methods of assembling a sensor for sensing biologically-active molecules by measuring impedance changes, are disclosed, comprising supporting a bio-sensor on a carrier, the bio-sensor comprising an electronic component having at least one micro-electrode and at least one electrical contact, functionalizing the bio-sensor by physically or chemically coupling a bio-receptor molecule to each of the at least one micro-electrode, and subsequently assembling the bio-sensor with a micro-fluidic unit by means of a clamp which clamps the bio-sensor with the micro-fluidic unit, such that in use a fluid introduced into the micro-fluidic unit is able to contact the bio-receptor and is isolated from the electrical contact. The clamp may be a spring, and the method may avoid a requirement for sealing by chemical or thermal means and thereby avoid damaging the bio-receptor. Sensors which can be assembled according to such methods are also disclosed.
    Type: Grant
    Filed: March 8, 2011
    Date of Patent: June 28, 2016
    Assignee: NXP B.V.
    Inventors: Romano Hoofman, Gerard Reuvers, Franciscus Petrus Widdershoven, Evelyne Gridelet, Marcus Henricus van Kleef
  • Patent number: 9322054
    Abstract: A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.
    Type: Grant
    Filed: February 21, 2013
    Date of Patent: April 26, 2016
    Assignees: Lockheed Martin Corporation, MICROLAB DIAGNOSTICS, INC.
    Inventors: Michael Egan, Brian Root, Orion N. Scott, Douglas J. South, Joan Bienvenue, Paul Kinnon, James Landers, David Saul, An-Chi Tsuei, Jason Hayes, Matthew Springer, Matthew Solomon, Peter van Ruijven
  • Patent number: 9285339
    Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: March 15, 2016
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Hongbo Peng, Stephen M. Rossnagel, Ajay K. Royyuru, Gustavo A. Stolovitzky, Philip S. Waggoner
  • Patent number: 9238835
    Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: January 19, 2016
    Assignee: Applied Biosystems, LLC
    Inventors: Hongye Sun, Steven Fung, Sam Lee Woo
  • Patent number: 9045795
    Abstract: A method of sensing nucleotide reactions includes flowing at least one nucleotide solution from a container of at least two containers of a sensor system. The sensor system includes a sensor sensitive to a byproduct of nucleotide incorporation. Each container of the at least two containers includes a different nucleotide solution. The sensor system enters an idle mode after flowing. The method further includes cycling the at least two containers through at least two cycles. Each cycle includes depressurizing the at least two containers for a first period and pressurizing the at least two containers for a second period. The method also includes pressurizing the at least two containers when the sensor system enters an active mode.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: June 2, 2015
    Assignee: Life Technologies Corporation
    Inventors: James Ball, David Kupec, David Marran, Jon A. Hoshizaki
  • Publication number: 20150140674
    Abstract: A polymer solution is dried until full evaporation of water. The polymer formed after drying the polymer solution is heated up and active degradation temperature range at a given heating rate and a degree of polymer degradation within this temperature range are determined. Then the solution is dried and a thermal analysis is conducted within the temperature range including the active degradation temperature range. A loss of mass of a portion of a sample of a porous medium is calculated and a loss of mass of similar sample of the porous medium sample after pumping the polymer solution injection is also determined. Based on the derived values, weight concentration of polymer penetrated into the porous medium is determined.
    Type: Application
    Filed: October 7, 2014
    Publication date: May 21, 2015
    Inventors: Dmitry Nikolaevich Mikhailov, Evgeny Mikhailovich Chuvilin, Lubov Vasilievna Melchakova, Tatyana Alexandrovna Buida
  • Publication number: 20150140576
    Abstract: The present invention describes glycans, which are specifically expressed by certain cancer cells, tumours and other malignant tissues. The present invention describes methods to detect cancer specific glycans as well as methods for the production of reagents binding to said glycans. The invention is also directed to the use of said glycans and reagents binding to them for the diagnostics of cancer and malignancies. Furthermore, the invention is directed to the use of said glycans and reagents binding to them for the treatment of cancer and malignancies. Moreover, the present invention comprises efficient methods to differentiate between malignant and benign tumors by analyzing glycan structures.
    Type: Application
    Filed: October 24, 2014
    Publication date: May 21, 2015
    Applicant: GLYKOS FINLAND OY
    Inventors: Tero SATOMAA, Jari NATUNEN, Annamari HEISKANEN, Anne OLONEN, Juhani SAARINEN, Noora SALOVUORI, Jari HELIN
  • Publication number: 20150132746
    Abstract: Provided herein is technology relating to the collection of biological samples and particularly, but not exclusively, to compositions, methods, and uses related to using a biopolymer substrate to collect biological samples for analysis.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 14, 2015
    Inventors: Astrid Gjelstad, Lars Erik Eng Eibak, Anne Bee Hegge, Knut Einar Rasmussen, Stig Pedersen-Bjergaard
  • Publication number: 20150132742
    Abstract: Microfluidic devices fabricated from paper that has been covalently modified to increase its hydrophobicity, as well as methods of making and using thereof are provided herein. The devices are typically small, portable, flexible, and both easy and inexpensive to fabricate. Microfluidic devices contain a network of microfluidic components, including microfluidic channels, microfluidic chambers, microwells, or combinations thereof, designed to carry, store, mix, react, and/or analyze liquid samples. The microfluidic channels may be open channels, closed channels, or combinations thereof. The microfluidic devices may be used to detect and/or quantify an analyte, such as a small molecules, proteins, lipids polysaccharides, nucleic acids, prokaryotic cells, eukaryotic cells, particles, viruses, metal ions, and combinations thereof.
    Type: Application
    Filed: June 3, 2013
    Publication date: May 14, 2015
    Inventors: Martin Mwangi Thuo, Ramses V. Martinez, Ana C. Glavan, Wenjie Lan, Xinyu Liu, Jean-Francis Bloch, George M. Whitesides
  • Patent number: 9029165
    Abstract: A method for detecting electromagnetic waves derived from bacterial DNA, comprising extracting and purifying nucleic acids from a sample; diluting the extracted purified nucleic acids in an aqueous solvent; measuring a low frequency electromagnetic emission over time from the diluted extracted purified nucleic acids in an aqueous solvent; performing a signal analysis of the low frequency electromagnetic emission over time; and producing an output, based on the signal analysis, in dependence on the DNA in the sample. The DNA may be extracted from at least one of blood, feces, urine, saliva, tears, seminal fluid, sweat, seminal and vaginal fluids of a patient, or water to determine, e.g., potability. The samples may be frozen. The extracting and purifying may comprise diluting the sample with an aqueous buffer and mixing; degrading proteins in the diluted sample; precipitating DNA from the buffer solution; and resuspending the precipitated DNA in an aqueous solution.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: May 12, 2015
    Inventor: Luc Montagnier
  • Publication number: 20150125897
    Abstract: The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) ofindividual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).
    Type: Application
    Filed: October 2, 2014
    Publication date: May 7, 2015
    Applicant: Ludwig Maximilians Universität München
    Inventors: Stefan DUHR, Philipp Baaske
  • Patent number: 9023655
    Abstract: Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: May 5, 2015
    Assignee: IBIS Biosciences, Inc.
    Inventors: Steven A. Hofstadler, Jared J. Drader, Jose R. Gutierrez, Paul J. Gleason, Rex O. Bare, Robert D. Miller, Jeffrey C. Smith
  • Patent number: 9023650
    Abstract: A simulant that includes a carrier and DNA encapsulated in the carrier. Also a method of making a simulant including the steps of providing a carrier and encapsulating DNA in the carrier to produce the simulant.
    Type: Grant
    Filed: November 1, 2013
    Date of Patent: May 5, 2015
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: George Roy Farquar, Roald N. Leif, Elizabeth Wheeler
  • Patent number: 9017970
    Abstract: The present invention relates to the discovery of RNA 5? polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.
    Type: Grant
    Filed: May 4, 2009
    Date of Patent: April 28, 2015
    Assignee: CellScript, LLC
    Inventors: Jerome J. Jendrisak, Ramesh Vaidyanathan, Ronald Meis
  • Publication number: 20150111234
    Abstract: A portable, hand-held glucose testing device includes a housing configured to accommodate a plurality of test sensors in a stacked arrangement and having a wall with an opening defined therein. A plurality of packaged test sensors is stacked in alignment with one another within the housing. Each of the test sensors is packaged within a blister package. The blister package includes a blister package housing and a cover foil overlying a surface of the blister package housing and the test sensor. A drive slide is configured to displace one of the plurality of packaged test sensors out of alignment with other packaged test sensors. A knife mechanism is configured to pierce through the cover foil, and to engage and urge the test sensor to extend through the opening for receiving a sample. A meter contact is configured to engage the test sensor when the test sensor extends through the opening.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 23, 2015
    Inventors: Steven C. Charlton, Allen Brenneman
  • Publication number: 20150111199
    Abstract: Methods of studying, interrogating, analyzing, and detecting particles, substances, and the like with near field light are described. Methods of identifying binding partners, modulators, inhibitors, and the like of particles, substances, and the like with near field light are described. In certain embodiments, the methods comprise immobilizing or trapping the particle, substance, and the like.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 23, 2015
    Inventors: Robert Hart, Bernardo Cordovez
  • Patent number: 9012229
    Abstract: A capillary electrophoresis method for quantitatively analyzing characteristic oligosaccharide present in enoxaparin sodium is provided in this invention. The method may be used for quantitatively determining the contents of disaccharides, trisaccharides, tetrasaccharides and in particular oligosaccharides having a 1,6-anhydro ring, which are unique compounds for enoxaparin sodium, within an exhaustively digested enoxaparin sodium sample with a mixture of heparinase I, II, and III, so as to quantitatively determine the molar percentage of oligosaccharides having 1,6-anhydro ring in enoxaparin sodium. The method may be used for the pharmaceutical quality control of enoxaparin sodium during the manufacturing process.
    Type: Grant
    Filed: January 20, 2012
    Date of Patent: April 21, 2015
    Assignees: Hangzhou Jiuyuan Gene Engineering Co., Ltd., Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
    Inventors: Jingwu Kang, Xueqiang Zhan
  • Patent number: 9012838
    Abstract: The present disclosure relates to functionalized nanodiamonds comprising at least one MALDI matrix covalently bonded to a nanodiamond and compositions comprising the same. The present disclosure also relates to methods of performing matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), for example on small molecules, using matrices comprising at least one MALDI matrix covalently bonded to a nanodiamond.
    Type: Grant
    Filed: April 21, 2014
    Date of Patent: April 21, 2015
    Assignee: University of Saskatchewan
    Inventors: Anas El-Aneed, Jackson Chitanda
  • Publication number: 20150104877
    Abstract: A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
    Type: Application
    Filed: November 4, 2013
    Publication date: April 16, 2015
    Applicant: International Business Machines Corporation
    Inventors: Natalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
  • Publication number: 20150104804
    Abstract: A method for manufacturing cubic diamond nanocrystals (10) comprising the following successive steps: (a) providing crystalline diamond powder where the maximum particle size of the powder is equal or more than 2 ?m and equal or less than 1 mm; (b) milling said crystalline micron diamond powder using nitrogen jet milling micronization so as to manufacture a fine powder; (c) nanomilling the fine powder of step b) using a planetary tungsten carbide ball mill; (d) acid treating the nanomilled powder of step c); (e) extracting the cubic diamond nanocrystals (10) by centrifugation. Advantageously round-shaped cubic diamond nanocrystals are manufactured.
    Type: Application
    Filed: December 18, 2014
    Publication date: April 16, 2015
    Inventors: Patrick Curmi, Jean-Paul Boudou, Alain Thorel, Fedor Jelezko, Mohamed Sennour
  • Patent number: 9005985
    Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: April 14, 2015
    Assignee: APDN (B.V.I.) Inc.
    Inventors: Thomas Kwok, Benjamin MingHwa Liang, Stephane Shu Kin So