Heterocyclic Carbon Compound (i.e., O, S, N, Se, Te, As Only Ring Hetero Atom) Patents (Class 436/91)
  • Patent number: 9714328
    Abstract: The present invention provides a method for preparing a sample characterized by binding a substance A containing a hydrazide group to a sugar chain and/or a sugar derivative via hydrazone formation between the hydrazide group of the substance A and the reducing end of the sugar chain and/or the sugar derivative thereby to enable the separation and purification of the sugar chain and/or the sugar derivative for an analytical sample from a biological sample containing the sugar chain and/or the sugar derivative by a simple operation.
    Type: Grant
    Filed: September 25, 2013
    Date of Patent: July 25, 2017
    Assignees: Sumitomo Bakelite Company, Ltd., National University Corporation Hokkaido University
    Inventors: Hideyuki Shimaoka, Shinichiro Nishimura, Yasuro Shinohara, Yoshiaki Miura, Jun-ichi Furukawa
  • Patent number: 9550185
    Abstract: The invention relates to a sample device (1) comprising an array of reaction chambers/wells (3) in a titer plate (2) and a two-dimensional array (10) of thin-film-light sensor elements (12), and each light-sensitive element (12) of the sensor array (10) is disposed directly underneath the individual reaction chamber (3) in order to detect chemical and/or biological reactions.
    Type: Grant
    Filed: November 8, 2007
    Date of Patent: January 24, 2017
    Assignee: ASMAG-Holding GmbH
    Inventor: Max Sonnleitner
  • Patent number: 9377468
    Abstract: Compound tags and shifting agents are provided that find use in ion mobility spectrometry (IMS), mass spectrometry (MS), or a combination of IMS and MS, and which can substantially increase separation of multiple components in complex samples and facilitate quantitative and multiplexed analyses. In some cases, the compounds include a linker and a normalizing group, each including a structural unit and separated by a cleaveable group, and a crown ether. Also provided are methods for analyzing peptides in a sample. In some cases, the method includes coupling the compound to peptides which include a terminal guanidinium moiety capable of forming an intra-molecular complex with the crown ether.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 28, 2016
    Assignee: Agilent Technologies, Inc.
    Inventors: Brian Phillip Smart, Joel Myerson, Craig Daniel Wenger, Javier E. Satulovsky
  • Patent number: 9018016
    Abstract: What is provided includes a porous sol-gel material whose intrinsic pH is lower than 1 and comprising at least one probe molecule chosen from the group consisting of croconic acid, p-dimethyl-aminobenzaldehyde (DMABA), p-dimethyl aminocinnamaldehyde (DMACA), p-methoxybenzaldehyde (MOB) and 4-methoxy-1-naphtaldehyde (MON). In addition, a detection system containing the porous sol-gel material and a method of preparation and use of the porous sol-gel material for trapping and/or detecting and optionally quantifying at least one chemical compound such as indole and indole compounds are provided.
    Type: Grant
    Filed: November 10, 2010
    Date of Patent: April 28, 2015
    Assignee: Commissariat a l'Energie Atomique et aux Energies Alternatives
    Inventors: Sabine Crunaire, Thu-Hoa Tran-Thi
  • Patent number: 9005985
    Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: April 14, 2015
    Assignee: APDN (B.V.I.) Inc.
    Inventors: Thomas Kwok, Benjamin MingHwa Liang, Stephane Shu Kin So
  • Patent number: 8999718
    Abstract: A vapochromic gold-copper complex [AuL2(Cu(Y)n)2](X)3 exhibiting luminescence is provided, where L is an N-heterocyclic carbene; Y is a heteroatom-containing ligand; X is an anion, and n is an integer having a value of 1 or 2, and solvates thereof. A reaction of [AuL2(Cu(Y)n)2](X)3 with water vapor or an organic compound vapor, for example, affords a modified complex that yields a change in luminescence color under UV excitation. These tricationic vapochromic materials exhibit large changes in the emission through ligand substitution reactions between the solid complex and vapors, which permit use in luminescent vapochromic sensors.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: April 7, 2015
    Assignee: Board of Regents of the Nevada System of Higher Education, on behalf of the University of Nevada, Reno
    Inventors: Vincent J. Catalano, Christoph E. Strasser
  • Patent number: 8999722
    Abstract: Sensors and methods for determination of analytes are provided. Analytes including explosives (e.g., RDX or PETN) may be determined by monitoring, for example, a change in an optical signal of a material upon exposure to the analyte. In some embodiments, the analyte and the material may interact via a chemical reaction to form a new emissive species. Embodiments described herein may provide inexpensive sensors with high selectivity and sensitivity.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: April 7, 2015
    Assignee: Massachusetts Institute of Technology
    Inventors: Timothy M. Swager, Trisha L. Andrew
  • Patent number: 8999720
    Abstract: Medical devices are typically sterilized in processes used to manufacture such products and their sterilization by exposure to radiation is a common practice. Radiation has a number of advantages over other sterilization processes including a high penetrating ability, relatively low chemical reactivity, and instantaneous effects without the need to control temperature, pressure, vacuum, or humidity. Unfortunately, radiation sterilization can compromise the function of certain components of medical devices. For example, radiation sterilization can lead to loss of protein activity and/or lead to bleaching of various dye compounds. Embodiments of the invention provide methods and materials that can be used to protect medical devices from unwanted effects of radiation sterilization.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: April 7, 2015
    Assignee: Medtronic MiniMed, Inc.
    Inventors: Jesper Svenning Kristensen, Soren Aasmul
  • Patent number: 8969092
    Abstract: Provided is a gel particle measurement reagent effective in quickly measuring a time point of initiation of production of gel particles. A gel particle measurement reagent R is a gel particle measurement reagent to be used to be agitated continuously with a sample S containing a target substance St as a measuring object to turn the target substance St into gel particles, including: a reagent base material 1 that undergoes a gelation reaction with the target substance St; and a biologically inactive particle formation accelerating factor 2 that is added to the reagent base material, has solubility in the sample S and dissolves therein at a concentration of 0.002 to 1%, and accelerates production of gel particles G whose particle sizes are centered in a predetermined range.
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: March 3, 2015
    Inventors: Toru Obata, Masakazu Tsuchiya
  • Patent number: 8969091
    Abstract: The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.
    Type: Grant
    Filed: February 13, 2013
    Date of Patent: March 3, 2015
    Assignee: President and Fellows of Harvard College
    Inventors: Jene A. Golovchenko, Jiali Li, Derek Stein, Marc H. Gershow
  • Patent number: 8949038
    Abstract: Described herein is a method of controlling bitumen quality in a process stream within a solvent-assisted bitumen extraction operation, for instance a hydrocarbon stream from a froth separation unit (FSU). Bitumen quality is a measure of the amount of selected contaminants in the process stream. Contaminants may include asphaltenes (comprising metal porphyrins), sulfur, and inorganic solids (comprising inorganic elements, e.g. Si, Al, Ti, Fe, Na, K, Mg, and Ca). First, the amounts of selected contaminants are measured. Next, these measured values are compared to maximum reference values. If one or more of these contaminants is higher than the maximum reference value, at least one variable of the solvent-assisted bitumen extraction is adjusted to improve bitumen quality.
    Type: Grant
    Filed: June 23, 2011
    Date of Patent: February 3, 2015
    Assignee: ExxonMobil Upstream Research Company
    Inventors: Tapantosh Chakrabarty, Ken N. Sury, Joseph L. Feimer
  • Patent number: 8932866
    Abstract: As a result of collecting blood from pancreatic cancer patients, esophageal cancer patients, and stomach cancer patients, and conducting mass spectrometry on N-linked sugar chains in the plasmas, sugar chains whose abundances are significantly different from those of healthy subjects have been successfully identified from the blood samples of the cancer patients.
    Type: Grant
    Filed: April 6, 2011
    Date of Patent: January 13, 2015
    Assignees: Kagoshima University, Sumitomo Bakelite Co., Ltd.
    Inventors: Shoji Natsugoe, Yasuto Uchikado, Teruto Hashiguchi, Hiroyuki Shinchi, Kosei Maemura, Yuko Mataki, Norichika Moriwaki, Masaru Sekijima, Hideyuki Shimaoka, Midori Abe, Masao Fukushima, Kota Igarashi, Hiroki Abe, Taichi Aihara
  • Patent number: 8932867
    Abstract: The present invention provides a high purity heparin useful to be a pharmaceutical product, cosmetics, research reagent, or the like, and a method for producing the same, more specifically, a heparin which does not substantially contain a nitrous acid degradation-resistant impurity and a method for producing a heparin, comprising mixing an aqueous solution of 5 to 30% by weight of the heparin with ethanol having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin.
    Type: Grant
    Filed: September 13, 2011
    Date of Patent: January 13, 2015
    Assignees: University of Miyazaki, Fuso Pharmaceutical Industries Ltd.
    Inventors: Michio Muguruma, Hiroshi Murata
  • Patent number: 8912003
    Abstract: Methods for quantitative determination of the presence of isothiazolone compounds in a solution include controlling pH of the solution in a range from about 3 to about 10, combining a known quantity of the pH-adjusted solution with a known quantity of an aromatic thiolate anion, quantitatively determining color intensity of the resultant combined solution; and correlating the color intensity with amount of isothiazolone compounds. The methods may be used in automated systems, including systems utilizing optical sensor devices.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: December 16, 2014
    Assignee: General Electric Company
    Inventor: David James Monk
  • Patent number: 8906647
    Abstract: Methods of inhibiting cytochrome P450 enzymes are provided that can be used for improving the treatment of diseases by preventing degradation of drugs or other molecules by cytochrome P450. Pharmaceutical compositions are provided that can act as boosters to improve the pharmacokinetics, enhance the bioavailability, and enhance the therapeutic effect of drugs that undergo in vivo degradation by cytochrome P450 enzymes.
    Type: Grant
    Filed: February 23, 2009
    Date of Patent: December 9, 2014
    Assignee: Sequoia Pharmaceuticals, Inc.
    Inventors: Michael Eissenstat, Dehui Duan
  • Patent number: 8895313
    Abstract: Provided are certain benzothiazole, imidazothiazole, imidazopyrimidine and imidazopyridine compounds, including, for example: formula (I) and pharmaceutically and physiologically acceptable salts, hydrates, and solvates thereof. Such compounds can be used as diagnostic ligands or labels of tau protein and PHF.
    Type: Grant
    Filed: September 23, 2009
    Date of Patent: November 25, 2014
    Assignee: Wista Laboratories Ltd.
    Inventors: Steven John Kemp, Lynda Jane Storey, John Mervyn David Storey, Janet Rickard, Charles Robert Harrington, Claude Michel Wischik, Scott Clunas, Tobias Kerst Heinrich
  • Patent number: 8871520
    Abstract: The invention provides a method of detecting uracil. The method comprises reacting uracil with a compound represented by the formula (I) in the presence of an oxidant and a base to produce a fluorescent compound represented by the formula (II).
    Type: Grant
    Filed: March 1, 2011
    Date of Patent: October 28, 2014
    Assignee: Nagasaki University
    Inventors: Masaaki Kai, Takayuki Shibata
  • Patent number: 8828732
    Abstract: The method of labeling a sugar chain from a biological sample employs a single reaction vessel for the sequential performance of the following steps of (a) isolating a sugar chain from a sample using a sugar-trapping substance; (b) washing the sugar-trapping substance having the sugar chains trapped thereon; (c) releasing the sugar chain from the sugar-trapping substance; and (d) labeling the released sugar chain with UV/visible or fluorescent compound having an amino group forming a stable labeled sugar in fewer steps than a conventional ion exchange based technique.
    Type: Grant
    Filed: April 28, 2009
    Date of Patent: September 9, 2014
    Assignee: Sumitomo Bakelite Co., Ltd.
    Inventors: Midori Abe, Hideyuki Shimaoka, Hiromitsu Kuramoto
  • Patent number: 8795595
    Abstract: A sensing apparatus may include a substrate having a first side for a sensing element and a second side for electronics, the substrate may have a at least one via from the first side of the substrate to the second side of the substrate, the at least one via may be hermetically sealed with an optically transmissive material.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: August 5, 2014
    Assignee: Medtronic Minimed, Inc.
    Inventor: Rajiv Shah
  • Patent number: 8785205
    Abstract: The present invention relates to assays for detection of nicotine metabolites, in particular cotinine, in fluid samples and uses of these assays in quantification of smoking habits. The assays comprise contacting a body fluid sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound which is associated with the presence of nicotine metabolites. Also provided are assay kits including a cyanogen halide or cyanogen halide-precursor(s) and a pyrazolone compound.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: July 22, 2014
    Assignee: GFC Diagnostics Ltd.
    Inventors: Charles John Knill, John Frederick Kennedy
  • Patent number: 8703497
    Abstract: A system and method for analyzing nucleic acids is presented herein. The system can include a nanowire assembly including a plurality of nanowires. In some embodiments, at least two nanowires can be coupled to a different probe corresponding to a different sequence version of a polymorphism. The system can include a reagent delivery system capable of delivering a template polymorphic nucleotide, an extension nucleic acid, and an enzyme to the nanowire assembly. The system can include an electrical detector configured to measure an electrical characteristic of the nanowire assembly and/or a controller in communication with the reagent delivery system. In some embodiments, the electrical detector can be configured to collect data corresponding to changes in the electrical characteristic in response to contact with the template polymorphic nucleotide, the extension nucleic acid, and the enzyme.
    Type: Grant
    Filed: June 4, 2012
    Date of Patent: April 22, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Hongye Sun, Steven Fung, Sam Woo
  • Patent number: 8703499
    Abstract: The application provides a laboratory. The laboratory (400) comprises a portable casing (404). The portable casing (404) comprises a tray unit (407), an actuator unit (405, 412), an analyzer unit (419), and a communication unit (402, 415, 438). The tray unit (407) is used for receiving a cartridge (409). The cartridge (409) comprises an analyte reservoir (430) for receiving an analyte fluid, one or more chemical reagent reservoirs (432) for storing one or more chemical reagent fluids, and one or more channels (434) connecting the chemical reagent reservoirs (432) with the analyte reservoir (430). The channel (434) comprises a measurement area (436) while the measure measurement area (436) comprises a sensor. The actuator unit (405, 412) is used for reducing the volume of the analyte reservoir (430) and for reducing the volume of the chemical reagent reservoirs (432). The analyzer unit (419) is used for measuring a physical value in the measurement area (436) using the sensor.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: April 22, 2014
    Assignee: E-Vitae Pte. Ltd.
    Inventor: Mehdi Khaled
  • Patent number: 8691588
    Abstract: A method is provided for fabricating a nanochannel. The method comprises providing a microchannel and controlling collapse of the microchannel so that it collapses to form a nanochannel of desired dimensions. The method employs a collapsible, flexible material such as the elastomer polydimethylsiloxane (PDMS) to form the nanochannel. A master is provided that is configured to have geometric conditions that promote a desired frequency of microchannel collapse. A collapsible material having a stiffness that also promotes a desired frequency of microchannel collapse is molded on the master. The molded collapsible material is removed from the master and bonded to a base, thereby forming the microchannel, which then collapses (or is collapsed) to form the nanochannel of desired dimensions. Nanofluidic and microfluidic devices comprising complex nanochannel structures and micro to nanochannel transitions are also provided.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: April 8, 2014
    Assignee: Cornell University
    Inventors: Seung-min Park, Yun Suk Huh, David Erickson, Harold G. Craighead
  • Patent number: 8673648
    Abstract: Disclosed is a method separation analysis of reducing sugars with enhanced sensitivity and an analytical apparatus therefore employing the post-column fluorescence detection/boric acid complex anion exchange method. The method disclosed is a method for analysis of reducing sugars using the post-column fluorescence detection/boric acid complex anion exchange method, and characterized in that a back-pressure generator is installed in the flow path between the heater, which is for causing a reaction by heating a sample separated by column chromatography with a basic amino acid, and a fluorometric detector.
    Type: Grant
    Filed: April 25, 2011
    Date of Patent: March 18, 2014
    Assignee: JCR Pharmaceuticals Co., Ltd.
    Inventor: Tetsuo Yokoyama
  • Patent number: 8663994
    Abstract: Analysis of compositions that include saccharides having mannosamine residues, such as the capsular saccharide of N. meningitidis serogroup A, is facilitated by a method comprising the steps of: (i) hydrolysing polysaccharide in the sample, to give a hydrolysate; (ii) subjecting the hydrolysate to liquid chromatography; and (iii) detecting any mannosamine-6-phosphate separated in step (ii).
    Type: Grant
    Filed: June 27, 2007
    Date of Patent: March 4, 2014
    Assignee: Novartis AG
    Inventor: Sandro D'Ascenzi
  • Patent number: 8609430
    Abstract: Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided.
    Type: Grant
    Filed: August 27, 2012
    Date of Patent: December 17, 2013
    Assignee: Ibis Biosciences, Inc.
    Inventors: Steven A. Hofstadler, Jared J. Drader, Jose R. Gutierrez, Paul J. Gleason, Rex O. Bare, Robert D. Miller, Jeffrey C. Smith
  • Patent number: 8604014
    Abstract: Combinations of PI3K inhibitor compounds having Formulas I and II and chemotherapeutic agents, including stereoisomers, geometric isomers, tautomers, metabolites and pharmaceutically acceptable salts thereof, are useful for treating hyperproliferative disorders such as cancer. Methods of using such combinations for in vitro, in situ, and in vivo diagnosis, prevention or treatment of such disorders in mammalian cells, or associated pathological conditions, are disclosed.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: December 10, 2013
    Assignees: Genentech, Inc., F. Hoffmann-La Roche AG
    Inventors: Marcia Belvin, Lori Friedman, Klaus Hoeflich, Deepak Sampath, Ulka Vijapurkar, Jeffrey Wallin, Leisa Johnson, Mallika Singh, Sonal Patel
  • Patent number: 8597956
    Abstract: An ultrasensitive method for detecting non-aromatic non-planar nitroamine analytes in a sample is provided.
    Type: Grant
    Filed: December 7, 2010
    Date of Patent: December 3, 2013
    Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventors: Itamar Willner, Ran Tel-Vered, Michael Riskin
  • Patent number: 8574916
    Abstract: A method of detecting melamine which includes providing a quantity of SERS-active particles and mixing the SERS-active particles with a solution containing melamine. The method further includes detecting a surface enhanced Raman spectrum of the melamine. The foregoing method may optionally include aggregating the SERS-active particles. Aggregation may occur before or after the SERS-active particles are mixed with a solution containing melamine. The method of detecting melamine may optionally include concentration of the SERS-active particles. The method may further include mixing a chaotropic agent having a higher affinity for a selected binding site than melamine into the solution containing melamine. The method may further include mixing a quantity of a SERS-active standard having a known SERS spectrum with the solution containing melamine and SERS-active particles. Assay apparatus and systems are also disclosed.
    Type: Grant
    Filed: November 17, 2009
    Date of Patent: November 5, 2013
    Assignee: Cabot Security Materials Inc.
    Inventors: Michael J. Natan, Richard Griffith Freeman, William E. Doering, Rebecca Stoermer Golightly
  • Patent number: 8563253
    Abstract: The present invention relates to a method for diagnosing hemolytic anemia or a predisposition thereof. It also relates to a method of determining whether a compound is capable of inducing hemolytic anemia in a subject and to a method of identifying a drug for treating hemolytic anemia. Furthermore, the present invention relates to a data collection comprising characteristic values of metabolites, a data storage medium comprising said data collection, and a system and a device for diagnosing hemolytic anemia. Finally, the present invention pertains to the use of a group of metabolites or means for the determination thereof for the manufacture of a diagnostic device or composition for diagnosing hemolytic anemia in a subject.
    Type: Grant
    Filed: June 4, 2007
    Date of Patent: October 22, 2013
    Assignee: Metanomics GmbH
    Inventors: Tilmann B. Walk, Ralf Looser, Michael Manfred Herold, Jan C. Wiemer, Alexandre Prokoudine, Edgar Leibold, Bennard van Ravenzwaay, Werner Mellert, Georgia Coelho Palermo Cunha, Eric Fabian, Volker Strauss
  • Patent number: 8541239
    Abstract: The invention provides an assay method for the detection of a primary amine analyze in an aqueous body fluid, which method comprises contacting a sample of said body fluid at a pH below 9.5 with a thiol and an unsaturated cis-dialdehyde and testing for the presence of a pyrrole reaction product.
    Type: Grant
    Filed: October 15, 2009
    Date of Patent: September 24, 2013
    Assignee: Oxtox Limited
    Inventors: Dimitrios Konstantinos Kampouris, Patrick Robinson Huddleston, Craig Edward Banks
  • Publication number: 20130204107
    Abstract: Disclosed are field effect transistor-based (FET-based) sensors for the rapid and accurate detection of analytes both in vivo and in vitro. The FET-based sensors can include a substrate, a channel disposed on the substrate, a source electrode and a drain electrode electrically connected to the channel, and a recognition element for an analyte of interest immobilized on the surface of the channel via a linking group. The distance between the recognition element and the channel can be configured such that association of the analyte of interest with the recognition element induces a change in the electrical properties of the channel. In this way, an analyte of interest can be detected by measuring a change in an electrical property of the channel. Also provided are devices, including probes and multi-well plates, incorporating the FET-based sensors.
    Type: Application
    Filed: January 23, 2013
    Publication date: August 8, 2013
    Applicant: THE OHIO STATE UNIVERSITY
    Inventor: THE OHIO STATE UNIVERSITY
  • Patent number: 8501489
    Abstract: This invention is related to the field of the prevention and treatment of kidney disease. The treatment of kidney disease may be tailored depending upon the need for, or expectation of, long-term dialysis. For example, prediction of long-term dialysis treatment can be determined by monitoring urine biomarkers related to the development of chronic kidney disease. For example, a normalized time course of hyaluronic acid can be used to determine whether a patient having suffered acute kidney injury will require long-term dialysis.
    Type: Grant
    Filed: September 23, 2009
    Date of Patent: August 6, 2013
    Assignee: University of Pittsburgh—Of The Commonwealth System of Higher Education
    Inventors: Kai Singbartl, John A. Kellum, Jr.
  • Patent number: 8501490
    Abstract: Polymeric sequence probes and methods are described that enhance the speed and sensitivity of detection of target analytes by combining a multiplicity of binding moieties specific for analyte, at least two of which are linearly arranged and optionally a multiplicity of detectable labels.
    Type: Grant
    Filed: April 19, 2010
    Date of Patent: August 6, 2013
    Assignee: The Regents of the University of California
    Inventors: Yong Chen, Suxian Huang
  • Patent number: 8486707
    Abstract: A computer-implemented method for confirming the nucleotide sequence of an oligonucleotide is provided. In certain embodiments, the method comprises: a) inputting the nucleotide sequence of an oligonucleotide; b) executing an algorithm that provides the predicted molecular formulas of fragments of the oligonucleotide; c) comparing the predicted m/z values of the predicted molecular formulas to experimentally-obtained m/z values obtained by analysis of the oligonucleotide by tandem mass spectrometry to determine if the predicted masses correspond with the experimentally-obtained masses. The method may be used, for example, to confirm the identity of an oligonucleotide after it is synthesized.
    Type: Grant
    Filed: April 25, 2011
    Date of Patent: July 16, 2013
    Assignee: Agilent Technologies, Inc.
    Inventors: Zoltan Timar, Gergo Hegedus
  • Patent number: 8455260
    Abstract: A method for determining a sequence of a biomolecule, the method including binding a plurality of uniform probes to a biomolecule fragment, creating a collection of binding signatures for the fragment with each binding signature representing a series of distances between binding sites within the fragment, and grouping the binding signatures into a plurality of signature clusters based at least in part on distances between the binding sites in each binding signature. For each binding signature in a first cluster, a potential successor binding signature is selected from signature clusters other than the first signature cluster, and one of the potential successor binding signatures is identified as a successor binding signature. The last two steps are repeated until the successor signature represents a terminal signature, resulting in a sequence of signatures representing at least a portion of the biomolecule.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: June 4, 2013
    Assignee: Massachusetts Institute of Technology
    Inventors: Peter Goldstein, Franco Preparata, Eli Upfal
  • Patent number: 8455265
    Abstract: A surface grafted conjugated polyelectrolyte (CPE) is formed by coupling a CPE by a coupling moiety to the surface of a substrate. The substrate can be of any shape and size, and for many uses of the surface grafted CPE, it is advantageous that the substrate is a nanoparticle or microparticle. Surface grafted CPEs are presented that use silica particles as the substrate, where a modified silane coupling agent connects the surface to the CPE by a series of covalent bonds. Two methods of preparing the surface grafted CPEs are presented. One method involves the inclusion of the surface being modified by the coupling agent and condensed with monomers that form the CPE in a grafted state to the substrate. A second method involves the formation of a CPE with terminal groups that are complimentary to functionality that has been placed on the surface of the substrate by reaction with a coupling agent. The surface grafted CPEs are also described for use as biosensors and biocides.
    Type: Grant
    Filed: February 29, 2008
    Date of Patent: June 4, 2013
    Assignee: STC.UNM
    Inventors: David G. Whitten, Sireesha Chemburu, Thomas Corbitt, Linnea Ista, Gabriel Lopez, Kirk S. Schanze, Motokatsu Ogawa, Eunkyung Ji
  • Publication number: 20130102049
    Abstract: Novel method and reagents for generating reversibly tagged saccharides, aldehydes, carboxyl acids, or orthoacetates useful in analytical and diagnostic applications are disclosed. Saccharides are coupled at the reducing end to tagging moieties comprising a reagent selected from a ortho-diaminobenzoic(DAB)-peptide, an aldo-imidazole or N-methylated aldo-imidazole, or an ortho-phenyldiamine (OPD) or substituted OPD. The tagged saccharide further comprising detectable or functional groups coupled to the tagging moiety are provided. Kits and reagents for chromatography and mass spectrometry are disclosed.
    Type: Application
    Filed: July 12, 2012
    Publication date: April 25, 2013
    Applicant: ACADEMIA SINICA
    Inventor: Wen-Bin YANG
  • Patent number: 8426214
    Abstract: System and method for capturing, concentrating, and detecting a diagnostic target in a liquid, comprising applying a magnetic field to a mixture comprising a co-aggregate in the liquid to provide a collected co-aggregate in the liquid, wherein the co-aggregate comprises a magnetic particle having a stimuli-responsive polymer attached thereto and a non-magnetic particle having a stimuli-responsive polymer and a diagnostic target attached thereto.
    Type: Grant
    Filed: June 14, 2010
    Date of Patent: April 23, 2013
    Assignee: University of Washington
    Inventors: Patrick S. Stayton, Michael Nash, Jriuan Lai
  • Patent number: 8426216
    Abstract: This invention pertains to methods for authenticating an article comprising tagging the article with light emitting optical reporter particles, and more specifically tagging the articles with up-converting phosphor particles (UCP), linked to nucleic acids of detectable sequence.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: April 23, 2013
    Assignee: APDN (B.V.I.), Inc.
    Inventors: Thomas Kwok, Ming-Hwa Liang, Stephane Shu Kin So
  • Patent number: 8426213
    Abstract: The present invention relates to providing improved hydrogen peroxide assays, as well as droplet actuators for conducting such assays. The droplet actuators of the invention may be used to conduct droplet-based hydrogen peroxide assays. They may also be associated with detectors for analyzing the results of the hydrogen peroxide assays of the invention. They may be provided as components of systems which control droplet operations and/or detection for conducting the hydrogen peroxide assays. Measurement by the detector may be used to quantify the presence of an analyte in a sample.
    Type: Grant
    Filed: March 5, 2008
    Date of Patent: April 23, 2013
    Assignee: Advanced Liquid Logic Inc
    Inventors: Allen E. Eckhardt, Vijay Srinivasan, Vamsee K. Pamula
  • Patent number: 8420400
    Abstract: A method for authenticating and verifying a pharmaceutical item to be genuine is described. The method for authenticating a tablet comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to a tablet or capsule. The method also comprises collecting a sample of the tablet and verifying the tablet is genuine by detecting the particular nucleic acid material.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: April 16, 2013
    Assignee: APDN (B.V.I.), Inc.
    Inventors: James A. Hayward, Ming-Hwa Liang, Stephane Shu-Kin-So
  • Patent number: 8415164
    Abstract: A method for authenticating and verifying an item to be genuine is described. The method for authenticating the item comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to ink within an ink cartridge or a toner compound within a toner housing. The method also comprises collecting a sample of either the ink or toner compound and verifying the ink or toner is genuine by detecting the particular nucleic acid material.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: April 9, 2013
    Assignee: APDN (B.V.I.) Inc.
    Inventors: James A. Hayward, Ming-Hwa Liang, John Davis
  • Patent number: 8415165
    Abstract: A method for authenticating and verifying garment to be genuine is described. The method for authenticating a garment comprises applying a particular nucleic acid material/marker associated with a particular sequence of nucleic acid bases to a dye or paint and applying the marker to the garment. The method also comprises collecting a sample from the garment and verifying the garment is genuine by detecting the particular nucleic acid material on or within the garment.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: April 9, 2013
    Assignee: APDN (B.V.I.), Inc.
    Inventors: Ming-Hwa Liang, Stephane Shu Kin So, James Hayward
  • Patent number: 8394640
    Abstract: The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.
    Type: Grant
    Filed: December 6, 2010
    Date of Patent: March 12, 2013
    Assignee: President and Fellows of Harvard College
    Inventors: Jene A. Golovchenko, Jiali Li, Derek Stein, Marc H. Gershow
  • Patent number: 8394643
    Abstract: The present application relates to copolymers having at least one optionally substituted fluoranthene as a first monomer unit and at least one optionally substituted pyrrole as a second monomer unit. The copolymer may, for example, emit green light when exposed to a blue or ultraviolet radiation. Methods of making the copolymer are also disclosed, as well as methods and apparatuses for producing light and detecting nitroaromatics using the copolymer.
    Type: Grant
    Filed: March 3, 2011
    Date of Patent: March 12, 2013
    Assignee: Tongji University
    Inventors: Xingui Li, Dunyin Gu, Meirong Huang
  • Patent number: 8377699
    Abstract: The present invention relates to a system and process for detection and/or qualitative and quantitative identification of the biological material, such as specific sequences of nucleic acids or proteins as antibodies, present in biological samples. The system is composed by one or more light sources (1) combined with one or more integrated optical photo sensors, or not, and various electronic components (4), necessary for obtaining/processing of the signal emitted by the metal nanoprobes functionalized with a solution of biological composite, as well as also a micro-controller and a microprocessor, fixed or portable. This photosensor structure is able to detect and to quantify the color variations produced by metal nanoprobes, being this preferentially gold, functionalized by oligonucleotides complementary to specific DNA/RNA sequences, proteins, as for instance antibodies and/or antigens related with certain disease, or other sample or solution of biological composite, that are to be investigated.
    Type: Grant
    Filed: September 7, 2007
    Date of Patent: February 19, 2013
    Assignee: Universidade Nova de Lisboa
    Inventors: Rodrigo Ferräo De Paiva Martins, Pedro Miguel Ribeiro Viana Baptista, Elvira Maria Correia Fortunato
  • Patent number: 8372648
    Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: February 12, 2013
    Assignee: APDN (B.V.I.), Inc.
    Inventors: Thomas Kwok, Ming-Hwa Liang, Stephane Shu Kin So
  • Patent number: 8367414
    Abstract: Methods and systems are provided that utilize measured isotopic abundance data to correlate a product to the synthesis process by which it was made. By utilizing the principles described herein, isotopic process profiles for synthetic products can be constructed, products of unknown origin can be inferentially identified to the processes by which they were made, and synthesis processes can be monitored for consistency of process parameters.
    Type: Grant
    Filed: May 30, 2007
    Date of Patent: February 5, 2013
    Inventor: John P. Jasper
  • Patent number: 8367417
    Abstract: The discovery that alterations in methylation, which can cause one or more genes on the single X chromosome in males to be partially silenced or overexpressed, constitute a predisposition to autism spectrum disorders is generally disclosed herein. These alterations provide the rationale and basis for methods to diagnose autism spectrum disorders.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: February 5, 2013
    Assignee: Greenwood Genetic Center, Inc.
    Inventors: Roger E. Stevenson, Julie R. Jones, Cindy D. Skinner, Michael J. Friez