By Measuring Catalytic Activity Patents (Class 506/11)
  • Patent number: 9797891
    Abstract: A method for determining a biological response of a target (41, 42) to a soluble candidate substance includes the steps: introducing a soluble candidate substance into a laminar flow of a buffer liquid (2) to form a candidate substance solute (3) having an initial concentration profile (31); dispersing the initial concentration profile (31) to form a dispersed concentration profile (32); directing the dispersed concentration profile (32) into a detection channel (12) to form a final symmetrical concentration profile (33) therein; introducing a target into the detection channel (12) to obtain a combined concentration profile including a constant target concentration profile overlying the final symmetrical concentration profile (33); holding in the detection channel (12) at least one half of the combined concentration profile; and optically scanning the combined concentration profile to detect an optical signal representative of the biological response of the target to the soluble candidate substance.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: October 24, 2017
    Assignee: F. Hoffmann-La Roche AG
    Inventors: Michael Werner, Rainer E. Martin, Remo Anton Hochstrasser
  • Patent number: 9683220
    Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
    Type: Grant
    Filed: June 2, 2016
    Date of Patent: June 20, 2017
    Assignee: Codexis, Inc.
    Inventors: Robert Osborne, Vesna Mitchell, Khin Yu Naing Htwe, Xiyun Zhang, Erika M. Milczek, Jeffrey C. Moore
  • Publication number: 20150140588
    Abstract: The present invention relates to variants with improved activity in an amide-bond reaction. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: July 3, 2012
    Publication date: May 21, 2015
    Applicant: Novozymes A/S
    Inventors: Werner Besenmatter, Allan Svendsen
  • Publication number: 20150125888
    Abstract: A process for enzymatically converting a furanoside substrate in a product of interest, includes contacting the substrate with an enzyme in presence of an alcohol acceptor, wherein the enzyme is preferably Araf51, and wherein the product is preferably an alkyl furanoside. The mutant Araf51 enzyme showing improved transglycosylation activity in comparison with the native wild-type (wt) Araf51 enzyme, and a method for screening the mutants are also described.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 7, 2015
    Applicants: ECOLE NATIONALE SUPERIEURE DE CHIMIE DE RENNES (ENSCR), CENTRE NATIOANAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
    Inventors: Richard Daniellou, Caroline Nugier-Chauvin, Vincent Ferrieres, Alize Pennec, Ilona Chlubnova
  • Publication number: 20150119284
    Abstract: Disclosed is a microcapsule encoded depending on the kind of a target substance included therein. The encoded microcapsule has a hydrophilic liquid core including the target substance and a hydrophobic shell surrounding the liquid core. The encoded microcapsule includes graphical codes introduced on the surface of the shell.
    Type: Application
    Filed: October 28, 2013
    Publication date: April 30, 2015
    Applicant: SNU R&DB FOUNDATION
    Inventors: Sunghoon KWON, Younghoon SONG, Taehong KWON, Daewon LEE, Junhoi KIM
  • Publication number: 20150118218
    Abstract: The disclosure relates to methods of detecting a neural injury biomarker in a biological sample. The method includes subjecting a biological sample to an assay disclosed that produces a measurable signal and detecting the measurable signal. The presence or absence of the measurable signal indicates the presence or absence of the neural injury biomarker in the sample, and thereby diagnosing a subject as having a neural injury. The disclosure further relates to methods of determining the state of a subject's neural injury. Further disclosed are systems and devices useful in carrying out the methods disclosed.
    Type: Application
    Filed: June 11, 2012
    Publication date: April 30, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Alexander J.A. Travis, Roy Cohen
  • Publication number: 20150111786
    Abstract: The invention provides methods of treating a meiotic kinase-associated disease, preferably the meiotic kinase HSET, by administering an inhibitor of the meiotic kinase. Preferably, the disease is associated with the presence of supernumerary centrosomes, such as cancer. Methods of inhibiting the growth of a tumor cell by contacting the cell with an inhibitor of a meiotic kinase, preferably HSET, are also provided. Screening methods for identifying inhibitors of the meiotic Kinase HSET are also provided. Methods of selecting subjects for treatment with an inhibitor of a meiotic kinase, such as HSET, are also provides.
    Type: Application
    Filed: January 7, 2015
    Publication date: April 23, 2015
    Applicant: DANA-FARBER CANCER INSTITUTE, INC.
    Inventor: David Pellman
  • Publication number: 20150111269
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 23, 2015
    Inventors: Yougen LI, Der-Ing LIAO, Mark J. NELSON, Daniel P. OKEEFE
  • Publication number: 20150111785
    Abstract: The present invention relates to a chromogenic method for simultaneously determining the activity of multiple coagulation proteases or for simultaneously determining the inhibition of multiple coagulation proteases in a single test reaction. For this purpose, use is made of two chromogenic substrates which have different absorption maxima and whose color signals can be separated spectrally.
    Type: Application
    Filed: January 5, 2015
    Publication date: April 23, 2015
    Inventor: Norbert Zander
  • Patent number: 9012166
    Abstract: Provided are a method for measuring histone methyltransferase activity, a method for screening for compounds that inhibit histone methyltransferase activity, a reagent kit for measuring histone methyltransferase activity, and a kit for screening for compounds that inhibit histone methyltransferase activity. A substrate compound represented by general formula (I): R1-X-K-R2 (I), or a salt thereof, wherein R1 represents a hydrogen atom or a protecting group for an amino terminus; X represents a peptide consisting of 0 or 1 or more amino acid residues; K represents a lysine residue; and R2 represents a dye label linked via an amide bond to the carbonyl terminus of a lysine residue, wherein the cleavage of the amide bond by peptidase changes the fluorescence property or chromogenic property of the dye label, and the methylation of the ? amino group of the lysine residue by the histone methyltransferase decreases susceptibility to peptidase, is used.
    Type: Grant
    Filed: April 15, 2011
    Date of Patent: April 21, 2015
    Assignee: Kyushu Institute of Technology
    Inventors: Norikazu Nishino, Yasushi Takemoto, Akihiro Ito, Minoru Yoshida
  • Patent number: 8999896
    Abstract: Screening assays that allow for the identification of agents that modulate the activity of N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway are provided. Also provided are methods of using an agent that modulates the activity of N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway to increase or decrease protein degradation in a cell, and to modulate physiologic and pathologic associated with N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway.
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: April 7, 2015
    Assignee: California Institute of Technology
    Inventors: Cheol-Sang Hwang, Anna Shemorry, Alexander Varshavsky
  • Publication number: 20150094327
    Abstract: The invention provides a fluorescence polarization (FP)-based assay to identify inhibitors of resolvase's DNA cleavage activity. The invention also provides resolvase inhibitors identified by the assay, as well as derivatives and analogs thereof. In certain embodiments, the compounds of the invention are useful to treat a poxvirus infection in an infected subject.
    Type: Application
    Filed: April 29, 2013
    Publication date: April 2, 2015
    Inventors: Frederic D. Bushman, Young Hwang, Matt Culyba
  • Publication number: 20150094229
    Abstract: The present invention concerns a method for the screening of antibacterial substances comprising a step of determining the ability of a candidate substance to inhibit the activity of a purified enzyme selected from the group consisting of: (i) a D-aspartate ligase comprising a polypeptide having an amino acid sequence possessing at least 50% amino acid identity with an amino acid sequence selected from the group consisting of SEQ ID No 1 to SEQ ID No 10, or a biologically active fragment thereof; and (ii) a L,D-transpeptidase comprising a polypeptide having an amino acid sequence possessing at least 50% amino acid identity with the amino acid sequence of SEQ ID No 11, or a biologically active fragment thereof.
    Type: Application
    Filed: October 7, 2014
    Publication date: April 2, 2015
    Inventors: Jean-Luc MAINARDI, Laurent GUTMANN, Michel ARTHUR, Samuel BALLAIS, Jean Emmanuel HUGONNET, Claudine MAYER, Sabrina BIAROTTE-SORIN
  • Publication number: 20150094211
    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.
    Type: Application
    Filed: September 30, 2014
    Publication date: April 2, 2015
    Inventors: Peter VANDER HORN, Daniel MAZUR, Theo NIKIFOROV, Mindy LANDES, Eileen TOZER
  • Publication number: 20150087552
    Abstract: In one aspect, the invention relates to a method for identifying a compound which modulates the activity of a voltage-gated protein. In certain embodiments, the voltage gate protein is a voltage-gated ion channel. In certain embodiments, the voltage-gated protein is a voltage sensitive phosphatase. In certain embodiments, the voltage-gated protein used in conjunction with the methods of the invention is modified to altered permeability or voltage sensitivity.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 26, 2015
    Inventors: Morten Ostergaard JENSEN, David BORHANI, VISHWANATH Jogini
  • Publication number: 20150080256
    Abstract: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents.
    Type: Application
    Filed: October 15, 2014
    Publication date: March 19, 2015
    Inventors: Michael R. GAMBINI, Jeff A. Levi, John C. Voyta, John G. Atwood, Bruce E. DeSimas, II, Edward J. Lakatos, Israel Metal, George Sabak, Yongdong Wang
  • Publication number: 20150072894
    Abstract: The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
    Type: Application
    Filed: September 17, 2014
    Publication date: March 12, 2015
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Stephen YUE, Gene SHEN, Wei-Chuan (David) SUN
  • Publication number: 20150065554
    Abstract: Disclosed herein are methods of treating a patient at risk of developing or having a neurofibromatosis or a sporadic schwannoma. In exemplary embodiments, the method involves administering to a subject in need an effective amount of a modulator of a target related to neurofibromatosis. Also disclosed are screening assays involving the implementation of Merlin-null Schwann cells, and to compounds identified using same.
    Type: Application
    Filed: March 13, 2013
    Publication date: March 5, 2015
    Applicant: University of Central Florida Research Foundation, Inc.
    Inventor: Cristina Fernandez-Valle
  • Publication number: 20150057185
    Abstract: Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
    Type: Application
    Filed: August 26, 2013
    Publication date: February 26, 2015
    Inventors: Christopher J. CHANG, Brian William Michel
  • Publication number: 20150051110
    Abstract: Provided herein are compositions and methods for identifying and detecting modulators of Ras protein conformational states through the use of second harmonic generation (SHG) technology. Also provided herein are methods for detecting a conformational changes in the three dimensional structure of a protein bound to a supported lipid bilayer.
    Type: Application
    Filed: November 2, 2012
    Publication date: February 19, 2015
    Inventors: Joshua S. Salafsky, Ryan P. McGuinness
  • Publication number: 20150051264
    Abstract: The present invention relates to modulators, in particular inhibitors, of the expression and/or the function of NADPH Oxidase 4 (Nox4) for use in the prevention and/or treatment of nerve injury, in particular pain, more particularly neuropathic pain. Further disclosed is a method for the identification of Nox4 modulators, a pharmaceutical composition comprising a Nox4 inhibitor and a method for preventing and treating pain, in particular neuropathic pain, in a subject in need of such a treatment. Also, the invention relates to modulators, in particular inhibitors, of the expression and/or the function of NADPH Oxidase 4 (Nox4) for use in the prevention and/or treatment of nerve injury associated with dysmyelination and methods for preventing and treating dysmyelination and diseases associated with dysmyelination.
    Type: Application
    Filed: September 14, 2012
    Publication date: February 19, 2015
    Applicant: FRAUNHOFER-GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG e.V.
    Inventors: Achim Schmidtko, Wiebke Kallenborn-Gerhardt, Gerd Geisslinger, Ralf Brandes, Katrin Schroeder
  • Publication number: 20150051097
    Abstract: Methods of screening for candidate compounds capable of inhibiting activity of fyn in phosphorylating tau protein at Y394 or binding to fyn to inhibit interaction with tau protein at Y394, including determining whether, and optionally the extent, the candidate compounds have these capabilities under conditions where fyn has these capabilities in the absence of the candidate compound. Methods of screening for substances capable of promoting dephosphorylation of tau protein by a phosphatase at a site of tau protein including contacting a candidate substance, the tau protein and a phosphatase capable of dephosphorylating the tau protein under conditions where the phosphatase is capable of dephosphorylating the site in absence of the candidate substance, where the kinase is fyn; determining whether, and optionally the extent, the candidate substance promotes dephosphorylation of the tau protein at the site; and selecting the candidate substance which promotes dephosphorylation of the tau protein the sites.
    Type: Application
    Filed: July 24, 2014
    Publication date: February 19, 2015
    Applicants: Proteome Sciences PLC, King's College London
    Inventors: Brian Anderton, Diane Hanger, Malcolm Ward, Helen Byers
  • Publication number: 20150038350
    Abstract: Means of determining the condition of the oral cavity in a subject is provided. The condition of the oral cavity in the subject is determined by using an analytical tool comprising the following (A), (B), and (C): (A) a reagent for measuring one or more parameters that reflect the dental caries risk for a test sample obtained from the oral cavity, (B) a reagent for measuring one or more parameters that reflect the periodontal disease risk for the test sample obtained from the oral cavity, and (C) a reagent for measuring one or more parameters that reflect the degree of oral cleanliness for the test sample obtained from the oral cavity.
    Type: Application
    Filed: December 27, 2011
    Publication date: February 5, 2015
    Applicant: ARKRAY, INC.
    Inventors: Eiji Nishinaga, Akira Uchiyama, Naho Suzuki, Tetsu Fukasawa, Riichi Maki, Akio Okubo, Isao Fukuta
  • Publication number: 20150038372
    Abstract: Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.
    Type: Application
    Filed: September 24, 2014
    Publication date: February 5, 2015
    Inventors: Gregory J. Sommer, Anson V. Hatch, Anup K. Singh, Ying-Chih Wang
  • Publication number: 20150031549
    Abstract: The invention relates to a method for selecting a sequence set from a library of expressed nucleic acid sequences, wherein cells are provided, each cell comprises an expressed nucleic acid sequence expressed as a target protein. The cells are encapsulated by treating them with a cationic polysaccharide and subsequently treating them with an anionic polysaccharide, yielding encapsulated cells, perforating the membrane of the encapsulated cells, yielding solubilized compartments, contacting them with a ligand to said target protein, the ligand bearing a detectable label, and selecting a subset of solubilized compartments as a function of detectable label and isolating the expressed nucleic acid sequences from the selection as a selected sequence set.
    Type: Application
    Filed: January 9, 2013
    Publication date: January 29, 2015
    Inventors: Daniel Scott, Andreas Plückthun
  • Publication number: 20150011421
    Abstract: An electrochemical method for measuring the activity of enzymes using nanoelectrode arrays fabricated with vertically aligned carbon nanofibers. Short peptide substrates specific to disease-related enzymes are covalently attached to the exposed nanofiber tips. A redox moiety, such as ferrocene, can be linked at the distal end of the nanofibers. Contact of the arrays with a biological sample containing one or more target enzymes results in cleavage of the peptides and changes the redox signal of the redox moiety indicating the presence of the target enzymes.
    Type: Application
    Filed: June 30, 2014
    Publication date: January 8, 2015
    Inventors: Jun Li, Duy Hua, Lateef Uddin Syed, Allan Prior, Luxi Swisher
  • Publication number: 20150011422
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS.
    Type: Application
    Filed: July 10, 2014
    Publication date: January 8, 2015
    Applicant: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Publication number: 20150004596
    Abstract: Provided herein in some embodiments is a non-naturally occurring variant of a wild type restriction enzyme defined by SEQ ID NO: 20, wherein the variant has at least a 2 fold increase in cleavage at 5-? glucosylhydroxymethylcytosine (5?ghmC) compared with methylcytosine relative to the wild type enzyme. Methods for examining hydroxymethylation of a DNA sample using the variant enzyme are also provided.
    Type: Application
    Filed: June 27, 2014
    Publication date: January 1, 2015
    Inventors: Zhenyu Zhu, Janine Borgaro, Aine Quimby, Shengxi Guan, Zhiyi Sun
  • Publication number: 20150005193
    Abstract: Disclosed herein are “equipment-free” flow-through assay devices based on patterned porous media, methods of making same, and methods of using same. The porous, hydrophilic media are patterned with hydrophobic barriers for performing assays on liquids.
    Type: Application
    Filed: June 20, 2014
    Publication date: January 1, 2015
    Inventors: Scott Thomas Phillips, Gregory Gerald Lewis, Jessica Sloane Robbins
  • Publication number: 20140357524
    Abstract: There is provided a method for quantifying a subject substance, of which typical examples are amino acids. The method of the present invention comprises the following steps: the step of allowing an enzyme that can generate pyrophosphate by using adenosine triphosphate (ATP) as a substrate with converting the subject substance to act on the subject substance to generate pyrophosphate; the step of allowing pyruvate pyrophosphate dikinase (PPDK) to act on the generated pyrophosphate in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate; and the step of quantifying the generated pyruvate, and amount of the subject substance is determined on the basis of the obtained amount of pyruvate. According to the present invention, an amino acid in a biological sample containing a lot of various kinds of contaminants such as inorganic phosphoric acid and urea can be conveniently and quickly quantified without being influenced by the contaminants.
    Type: Application
    Filed: August 6, 2014
    Publication date: December 4, 2014
    Applicants: AJINOMOTO CO., INC., TOYAMA PREFECTURE
    Inventors: Yasuhisa Asano, Masafumi Kameya
  • Publication number: 20140357523
    Abstract: A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also provided is a composition comprising at least 100 Cas9-associated guide RNAs that are each complementary to a different, pre-defined, site in a genome. Kits for performing the method are also provided. In addition, other methods, compositions and kits for manipulating nucleic acids are also provided.
    Type: Application
    Filed: May 29, 2014
    Publication date: December 4, 2014
    Applicant: Agilent Technologies, Inc.
    Inventors: Gusti Zeiner, Derek Lee Lindstrom, Brian Jon Peter, Robert A. Ach
  • Publication number: 20140348813
    Abstract: Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
    Type: Application
    Filed: January 28, 2014
    Publication date: November 27, 2014
    Applicants: THE REGENTS OF THE UNIVERSITY OF MICHIGAN, The Trustees of Columbia University in the City of New York
    Inventors: Donald W. Landry, James H. Woods, Roger K. Sunahara, Diwahar L. Narasimhan, Joanne MacDonald, Milan N. Stojanovich, John J. Tesmer, Remy L. Brim
  • Publication number: 20140341881
    Abstract: This invention relates to therapeutic compounds which are inhibitors of serine proteases, to pharmaceutical compositions thereof and to their use in the treatment of the human or animal body. More specifically, the present invention relates to a method for the treatment, diagnosis or prognosis of skin diseases comprising the administration to a subject in need thereof of a therapeutically effective amount of a Serine protease inhibitor.
    Type: Application
    Filed: July 30, 2014
    Publication date: November 20, 2014
    Inventors: David Deperthes, Christoph Kundig, Alain Hovnanian, Celine Deraison
  • Publication number: 20140336236
    Abstract: Novel ALK and NTRK1 fusion molecules and uses are disclosed.
    Type: Application
    Filed: April 21, 2014
    Publication date: November 13, 2014
    Applicant: FOUNDATION MEDICINE, INC.
    Inventors: Maureen T. Cronin, Doron Lipson, Roman Yelensky
  • Publication number: 20140336079
    Abstract: Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.
    Type: Application
    Filed: July 30, 2014
    Publication date: November 13, 2014
    Inventors: Brian AGNEW, Maura Ford, Kyle Gee, Kapil Kumar
  • Publication number: 20140327158
    Abstract: The present invention provides a screen which exploits mechanism-based activity probes that specifically and covalently modify the active site cysteine thiol residue of E3 Ub ligases including, but not limited to the HECT and RBR family. The activity probes are used to screen for activators and inhibitors of E3 Ub ligases, and to interrogate the functional state of E3 Ub ligases in human disease.
    Type: Application
    Filed: May 2, 2014
    Publication date: November 6, 2014
    Applicant: E3X BIO
    Inventors: Brigit Riley, Jennifer Johnston, David Morgans
  • Publication number: 20140329787
    Abstract: The present invention relates to a novel Phytophthora phospholipase C and uses thereof, methods of identifying modulators and inhibitors of a biological function of the phospholipase C, and methods of inhibiting Phytophthora growth comprising inhibiting a biological function of a novel Phytophthora phospholipase C.
    Type: Application
    Filed: October 17, 2012
    Publication date: November 6, 2014
    Inventors: Dean Ian Phillips, Peter Luke Beech
  • Publication number: 20140323334
    Abstract: The invention relates to quality control of analytical assays, particularly NAT assays of blood samples containing nucleic acids. A control panel containing quantified amounts of substances known to interfere with an analytical assay is used and compared with a reference sample in the analytical assay. A comparison of the assay results interference panel validates the assay and can serve as a periodic quality control check for the analytical assay as well as related methods and protocols. The use of the control panel of the invention can also determine whether interfering substances are present and establish under what conditions the analytical assay reliable.
    Type: Application
    Filed: May 5, 2014
    Publication date: October 30, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventor: Michael J. ECK
  • Publication number: 20140323339
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS.
    Type: Application
    Filed: July 10, 2014
    Publication date: October 30, 2014
    Applicant: FLUIDIGM CANADA INC.
    Inventor: Olga Ornatsky
  • Publication number: 20140315741
    Abstract: This document relates to methods and materials involved in modulating deubiquitinases (e.g., USP10 polypeptides) and/or ubiquitinated polypeptides (e.g., tumor suppressor polypeptides or mutant versions of tumor suppressor polypeptides). For example, methods and materials for increasing deubiquitinase (e.g., a USP10 polypeptide) expression or activity, methods and materials for decreasing deubiquitinase (e.g., a USP10 polypeptide) expression or activity, methods and materials for stabilizing tumor suppressor polypeptides (e.g., wild-type p53 polypeptides), methods and materials for de-stabilizing mutant versions of tumor suppressor polypeptides (e.g., mutant p53 polypeptides), and methods and materials for reducing cancer cell proliferation, increasing cancer cell apoptosis, and/or treating cancer (e.g., cancers having reduced levels of wild-type p53 polypeptides or cancers having increased levels of mutant p53 polypeptides) are provided.
    Type: Application
    Filed: July 1, 2014
    Publication date: October 23, 2014
    Inventors: Zhenkun Lou, Jian Yuan
  • Patent number: 8865708
    Abstract: Compounds of Formulas I-XLIII are identified as direct inhibitors of p97 ATPase or of the degradation of a p97-dependent ubiquitin-proteasome system (UPS) substrate. Methods and compositions are disclosed for inhibiting p97 ATPase and the degradation of a p97-dependent UPS substrate, and for identifying inhibitors thereof.
    Type: Grant
    Filed: May 6, 2011
    Date of Patent: October 21, 2014
    Assignees: California Institute of Technology, The University of Kansas, Cleave Biosciences, Inc.
    Inventors: Raymond J. Deshaies, Tsui-Fen Chou, Frank J. Schoenen, Kelin Li, Kevin J. Frankowski, Jeffrey Aube, Samuel W. Gerritz, Han-Jie Zhou
  • Publication number: 20140309141
    Abstract: Methods and compositions for detection of the modulators of proteolytic enzymes, particularly cysteine proteases, are disclosed.
    Type: Application
    Filed: September 21, 2012
    Publication date: October 16, 2014
    Inventors: Craig Leach, James Strickler, Michael Eddins
  • Publication number: 20140309172
    Abstract: The present disclosure identifies caspase-1/ICE as a therapeutic target for ?-synuclein associated diseases and disorders. Related methods and compositions are also provided.
    Type: Application
    Filed: November 4, 2011
    Publication date: October 16, 2014
    Inventors: Dagmar Ringe, Gregory A. Petsko, Quyen Hoang
  • Publication number: 20140309135
    Abstract: A method of screening a compound having a hypoglycemic effect (hereinafter referred to as “hypoglycemic compound”), a remedy for diabetes which contains a compound having a novel function mechanism, etc. More specifically speaking, a method of screening a hypoglycemic compound capable of binding to the ? subunit of a trimeric GTP-binding protein, a remedy for diabetes comprising a hypoglycemic compound, which is characterized by being capable of binding to the ? subunit of a trimeric GTP-binding protein, as the active ingredient, etc.
    Type: Application
    Filed: April 25, 2014
    Publication date: October 16, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Naoyuki FUKUCHI, Satoru Okamoto, Wataru Miyanaga, Sen Takeshita, Masaru Takayanagi, Yumiko Fukuda, Takao Ikenoue, Naoyuki Yamada, Naoko Arashida
  • Publication number: 20140303019
    Abstract: A microfluidic sensor includes a microchannel that includes a reaction site with a reagent and a sample inlet. A liquid substance is received at the sample inlet and travels by capillary action to the reaction site. A temperature sensor measures a temperature as a result of a reaction between the reagent and a chemical in the liquid substance. A controller is communicatively connected to the temperature sensor, receives the temperature measured by the temperature sensor, and derives a concentration of the chemical in the liquid substance from the temperature.
    Type: Application
    Filed: March 31, 2014
    Publication date: October 9, 2014
    Applicant: MARQUETTE UNIVERSITY
    Inventors: Chung Hoon Lee, Benyamin Davaji
  • Publication number: 20140294846
    Abstract: The present invention relates to ADAMTS-1 and uses thereof. The present invention also relates to fragments of ADAMTS-1 and methods of inhibiting cell growth and metastasis. The present invention also provide methods of identifying inhibitors and activators relating to the function of ADAMTS-1.
    Type: Application
    Filed: February 18, 2014
    Publication date: October 2, 2014
    Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventor: Qin Yu
  • Publication number: 20140296205
    Abstract: The invention provides compounds with enhanced permeability for selectively inhibiting glycosidases, prodrugs of the compounds, and pharmaceutical compositions including the compounds or prodrugs of the compounds. The invention also provides methods of treating diseases and disorders related to deficiency or overexpression of O-GlcNAcase, accumulation or deficiency of O-GlcNAc.
    Type: Application
    Filed: June 27, 2012
    Publication date: October 2, 2014
    Applicants: MERCK SHARP & DOHME CORP., ALECTOS THERAPEUTICS INC.
    Inventors: Tong-Shuang Li, Ernest J. Mceachern, David J. Vocadlo, Yuanxi Zhou, Yongbao Zhu, Harold G. Selnick
  • Publication number: 20140296357
    Abstract: Provided are a support for supporting a metal, a metal-supported catalyst, a methanation reaction apparatus, and a method relating thereto that realize effective methanation of carbon monoxide. The support for supporting a metal includes a carbonized material obtained by carbonizing raw materials containing an organic substance and a metal, in which the support is used for supporting a metal that exhibits a catalytic activity for a methanation reaction of carbon monoxide. The metal-supported catalyst includes: a support formed of a carbonized material obtained by carbonizing raw materials containing an organic substance and a metal; and a metal that exhibits a catalytic activity for a methanation reaction of carbon monoxide, the metal being supported on the support.
    Type: Application
    Filed: November 1, 2012
    Publication date: October 2, 2014
    Applicant: National University Corporation gunma University
    Inventors: Jun-ichi Ozaki, Hiroki Takahashi, Takuya Takahashi, Naokatsu Kannari, Rieko Kobayashi, Naoto Saito
  • Publication number: 20140288007
    Abstract: The disclosure relates to compounds, peptides or peptidomimetics that inhibit, reduce or prevent protease activity and the use of these compounds, peptides or peptidomimetics to treat or prevent a condition. In particular the condition may be periodontal disease. The protease activity may be activity of a gingipain. The compounds, peptides or peptidomimetics of the invention may also be used in assays for the identification of protease inhibitors.
    Type: Application
    Filed: November 2, 2012
    Publication date: September 25, 2014
    Applicant: Oral Health Australia Pty. Ltd.
    Inventors: Stuart Geoffrey Dashper, Eric Charles Reynolds, Noorjahan Laila Huq, Elena Chiew Yeen Toh
  • Publication number: 20140274797
    Abstract: The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme.
    Type: Application
    Filed: March 12, 2014
    Publication date: September 18, 2014
    Inventors: Zygmunt Gryczynski, Ignacy Gryczynski, Rafal Fudala, Badri P. Maliwal