Method Of Screening A Library Patents (Class 506/7)
  • Patent number: 11142793
    Abstract: Sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising two electrodes separated by an insulating layer are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the insulating region. A sequencing reaction mixture comprising nucleotide analogs having impedance labels is introduced to the array of nanoscale electronic elements under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance.
    Type: Grant
    Filed: October 30, 2019
    Date of Patent: October 12, 2021
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Stephen Turner, Jeremiah Hanes, Keith Bjornson
  • Patent number: 11111531
    Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: September 7, 2021
    Assignee: TANGEN BIOSCIENCES, INC.
    Inventors: John R. Nobile, John Davidson
  • Patent number: 11104950
    Abstract: Panels of 8-, 9- and 12-biomarker for diagnostic and prognostic methods to determine a subject's radiation exposure and discriminates between persons who have been exposed to radiation only, inflammation stress only, or a combination of the two.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: August 31, 2021
    Assignee: The Regents of the University of California
    Inventors: Andrew J. Wyrobek, Antoine M. Snijders
  • Patent number: 11097247
    Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: August 24, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Nicholas Heredia
  • Patent number: 11078543
    Abstract: Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: August 3, 2021
    Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
  • Patent number: 11072826
    Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: July 27, 2021
    Assignee: Natera, Inc.
    Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko
  • Patent number: 11061045
    Abstract: A matrix droplet extruder includes one or a plurality of reagent containers. Pneumatic connectors are each connectable to a corresponding docking station connector of a docking station that is connectable to a pneumatic generator controllable by a controller to provide pneumatic pressure to the pneumatic docking station connectors. A droplet matrix extrusion surface includes an array of perforations. A liquid management chip has a network of dispensing channels for dispensing reagents from reagent containers through the array of perforations. A pneumatic control network includes pneumatic channels and gates that are controllable by application of the pneumatic pressure to the gates via the pneumatic channels to enable or block dispensing the one or more reagents to repeatedly generate a matrix of droplets when applying the pneumatic pressure to the reagents.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: July 13, 2021
    Assignee: Picodya Technologies Ltd.
    Inventor: Yehuda Yavets-Chen
  • Patent number: 11047013
    Abstract: Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: June 29, 2021
    Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
  • Patent number: 11021757
    Abstract: There is a need for improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer, especially lymphoid neoplasms, such as lymphomas and leukemias. Provided herein are methods for using DNA sequencing to identify personalized, or patient-specific biomarkers in patients with lymphoid neoplasms, autoimmune disease and other conditions. Identified biomarkers can be used to determine and/or monitor the disease state for a subject with an associated lymphoid disorder or autoimmune disease or other condition. In particular, the invention provides a sensitive method for monitoring lymphoid neoplasms that undergo clonal evolutions without the need to development alternative assays for the evolved or mutated clones serving as patient-specific biomarkers.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: June 1, 2021
    Assignee: Adaptive Biotechnologies Corporation
    Inventors: Malek Faham, Thomas Willis
  • Patent number: 11016082
    Abstract: Provided is a non-invasive method of detecting or screening for a cancer in a subject specimen originating in a tissue outside of the lung. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for the cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for the cancer in the subject specimen.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: May 25, 2021
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Xiaoan Fu, Michael Nantz, Michael Bousamra, Victor van Berkel
  • Patent number: 10994275
    Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: May 4, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Michael Pallas, Evan Foster, Jorge Fonseca, James Nurse, Theodore Straub
  • Patent number: 10961583
    Abstract: The disclosure provides methods of identifying a human subject as a candidate for treating or inhibiting a liver disease by inhibiting HSD17B13. The disclosure also provides methods of treating a subject who is PNPLA3 Ile148Met+ by administering an inhibitor of HSD17B13. The disclosure also provides method of detecting a PNPLA3 Ile148Met variant and functional HSD17B13 in a subject. The disclosure also provides method of identifying a subject having a protective effect against liver disease. The disclosure also provides inhibitors of HSD17B13 for use in the treatment of a liver disease.
    Type: Grant
    Filed: October 11, 2018
    Date of Patent: March 30, 2021
    Assignee: Regeneron Phramaceuticals, Inc.
    Inventors: Yurong Xin, Jesper Gromada, Xiping Cheng, Frederick Dewey, Tanya Teslovich Dostal, Claudia Schurmann, Aris Baras, Noura Abul-Husn
  • Patent number: 10964409
    Abstract: Provided herein are methods, processes, systems and machines for non-invasive assessment of genetic variations.
    Type: Grant
    Filed: October 2, 2014
    Date of Patent: March 30, 2021
    Assignee: Sequenom, Inc.
    Inventor: Gregory Hannum
  • Patent number: 10956806
    Abstract: A computer-implemented method for efficient assembly of oligonucleotides for nucleic acid based data storage includes receiving encoded data including binary data encoded into nucleic acid sequence data, and assembling a target nucleic acid data strand based on the encoded data by, concatenating one or more selected codeword oligonucleotides obtained from a codeword stack strand.
    Type: Grant
    Filed: June 10, 2019
    Date of Patent: March 23, 2021
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Koji Masuda, Eiji Nakamura
  • Patent number: 10907196
    Abstract: The present application relates to new coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: January 9, 2020
    Date of Patent: February 2, 2021
    Assignee: Illumina Cambridge Limited
    Inventor: Nikolai Nikolaevich Romanov
  • Patent number: 10878703
    Abstract: The present invention provides agents comprising or consisting of a binding moiety with specificity for interleukin-1 receptor accessory protein (IL1RAP) for use in inducing cell death and/or inhibiting the growth and/or proliferation of pathological stem cells and/or progenitor cells associated with a neoplastic hematologic disorder, wherein the cells express IL1RAP. A related aspect of the invention provides agents comprising or consisting of a binding moiety with specificity for interleukin-1 receptor accessory protein (IL1RAP) for use in detecting pathological stem cells and/or progenitor cells associated with a neoplastic hematologic disorder, wherein the cells express IL1RAP. Further provided are pharmacological compositions comprising the agents of the invention and methods of using the same.
    Type: Grant
    Filed: May 21, 2018
    Date of Patent: December 29, 2020
    Assignee: Cantargia AB
    Inventors: Thoas Fioretos, Marcus Järås
  • Patent number: 10794898
    Abstract: Methods for identifying a compound that alters fluorescence resonance energy transfer (FRET) of a protein. The methods include use of a genetically engineered cell that includes a target protein. The target protein includes one or more heterologous domains. In one embodiment, a target protein includes two heterologous domains, and in another embodiment, the target protein includes a heterologous domain and the cell further includes a second protein that includes a heterologous domain. A heterologous domain may include a chromophore or an amino acid to which a fluorescent dye attaches. The fluorescence lifetime of one or more chromophore, one or more fluorescent dye, or the combination thereof, is measured after contacting the cell with a compound A difference between the fluorescence lifetime in the presence of the test compound and the fluorescence lifetime in the absence of the test compound indicates that the test compound alters the FRET of the target protein.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: October 6, 2020
    Assignees: Regents of the University of Minnesota, Loyola University Chicago
    Inventors: David D. Thomas, Simon Joseph Gruber, Razvan L. Cornea, Gregory David Gillispie, Kurt C. Peterson, Seth Louis Robia
  • Patent number: 10774370
    Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: September 15, 2020
    Assignee: 10X Genomics, Inc.
    Inventors: Xinying Zheng, Serge Saxonov, Michael Schnall-Levin, Kevin Ness, Rajiv Bharadwaj
  • Patent number: 10746738
    Abstract: The present invention is directed to claudin-1-specific peptide reagents, methods for detecting pre-cancer (dysplasia), early cancer and/or cancer using the peptide reagents, and methods for targeting pre-cancerous (dysplastic) cells, and/or cancer cells using the peptide reagents.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: August 18, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Thomas D. Wang, Emily Rabinsky, Bishnu P. Joshi
  • Patent number: 10734117
    Abstract: Apparatuses (including devices and systems) and methods for determining if a patient will respond to a variety of cancer drugs.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: August 4, 2020
    Assignee: STRAND LIFE SCIENCES PRIVATE LIMITED
    Inventors: Vaijayanti Gupta, Manimala Sen, Satish Sankaran, Kalyanasundaram Subramanian, Ramesh Hariharan, Vamsi Veeramachaneni, Shanmukh Katragadda, Rohit Gupta, Radhakrishna Bettadapura, Anand Janakiraman, Arunabha Ghosh, Smita Agrawal, Sujaya Srinivasan, Bhupender Singh, Urvashi Bahadur, Shuba Krishna, Mahesh Nagarajan, Nimisha Gupta, Sudhir Borgonha
  • Patent number: 10730030
    Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Nicholas Heredia
  • Patent number: 10725382
    Abstract: The present invention provides a system for conservation and efficient use of energy through controlling and monitoring of devices. At least one processing controller connected to a sensor and a device, the processing controller configured to receive the ambient data from the sensor and operating parameters from the device; a user module configured to IO receive input parameters from a plurality of users; a central processing module, connected to the structure, the user module, and the admin module through wired and/or wireless connection, the central processing module configured to process the data received from the processing controller adapted in the zone of the structure and generate the optimum parameters for operating the device adapted in the zone to the structure.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: July 28, 2020
    Assignee: Saint Louis University
    Inventors: Silviya Petrova Zustiak, Naveed Zafar Ahmed
  • Patent number: 10724092
    Abstract: The disclosure provides method and kits for characterizing spliced m RNA isoforms. The disclosure also provides methods of screening for mutations and oligonucleotides that modulate splicing.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: July 28, 2020
    Assignee: Erasmus University Medical Center Rotterdam
    Inventors: Atze Jacobus Bergsma, Erik van der Wal, Wilhelmus Wenceslaus Matthias Pijnappel, Antje Tjitske van der Ploeg, Arnoldus Reuser
  • Patent number: 10676776
    Abstract: A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.
    Type: Grant
    Filed: August 3, 2015
    Date of Patent: June 9, 2020
    Assignee: QUANTAMATRIX INC.
    Inventors: Sunghoon Kwon, Yong-Gyun Jung, Jung Il Choi, Hun Jong Na
  • Patent number: 10643738
    Abstract: Disclosed herein are methods, systems, and apparatus for detecting microamplifications or microdeletions in the genome of a fetus. In some embodiments, the method comprises receiving sequence tags for each of a plurality of DNA fragments in a biological sample; determining genomic positions for the sequence tags; determining whether the density of DNA in each of a plurality of genomic regions is aberrantly high or low; identifying as a microamplification a set of consecutive genomic regions having aberrantly high density; and identifying as a microdeletion a set of consecutive genomic regions having aberrantly low density. The biological sample may be a blood sample obtained noninvasively from a female subject pregnant with the fetus.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 5, 2020
    Assignee: The Chinese University of Hong Kong
    Inventors: Yuk Ming Dennis Lo, Kwan Chee Chan, Peiyong Jiang, Cheuk Yin Jandy Yu, Rossa Wai Kwun Chiu
  • Patent number: 10619206
    Abstract: Described herein are improved methods, compositions and kits for next generation sequencing (NGS). The methods, compositions and kits described herein enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (which can comprise regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information can be obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein can be useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: April 14, 2020
    Assignee: Tecan Genomics
    Inventors: Doug Amorese, Benjamin G. Schroeder, Jonathan Scolnick
  • Patent number: 10619205
    Abstract: A kit for use with a nucleic acid sequencing instrument can include a plurality of combinatorial barcodes sequences meeting the following criteria: each of the combinatorial barcode sequences comprise a plurality of iterations of a sequence motif, where the sequence motif comprises a first nucleotide base from a first group of nucleotide bases followed by a second nucleotide base from a second group of nucleotide bases, the first group and the second group differing from each other; and the plurality of combinatorial barcode sequences is at least 1,000,000 different barcode sequences.
    Type: Grant
    Filed: May 5, 2017
    Date of Patent: April 14, 2020
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Earl Hubbell
  • Patent number: 10578633
    Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: March 3, 2020
    Assignee: FLUIDIGM CORPORATION
    Inventors: Jason A. A. West, Brian Fowler
  • Patent number: 10564107
    Abstract: This disclosure provides methods and devices for the label-free detection of target molecules of interest. The principles of the disclosure are particularly applicable to the detection of biological molecules (e.g., DNA, RNA, and protein) using standard SiO2-based microarray technology.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: February 18, 2020
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: M. Selim Unlu, David A. Bergstein, Michael F. Ruane, Bennett B. Goldberg
  • Patent number: 10550431
    Abstract: The technology described herein is directed to the diagnosis, prognosis, and treatment of infection, e.g. after burn injury.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: February 4, 2020
    Assignee: THE GENERAL HOSPITAL CORPORATION
    Inventors: Laurence Rahme, Shuangchun Yan, Yok-Ai Que, Amy Tsurumi
  • Patent number: 10466215
    Abstract: The invention discloses a method for predicting the solubility of at least one species at a specified pH value in an aqueous buffer comprising at least one weak acid species and/or at least one weak base species.
    Type: Grant
    Filed: September 22, 2015
    Date of Patent: November 5, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Enrique Carredano, Elenor Strandberg
  • Patent number: 10457987
    Abstract: Provided are methods and kits that allow the amplification, in a single multiplex reaction, of the thirteen rapidly mutating Y chromosome short tandem repeats (RM Y-STRs) at loci DYF387S1; DYF399S1; DYF403S1a/b; DYF404S1; DYS449; DYS518; DYS526a/b; DYS547; DYS570; DYS576; DYS612; DYS626; and DYS627, if present in a sample of DNA, and determination of the alleles at these RM Y-STRs. The ability to achieve such determination through a single multiplex arises as a result of a beneficially designed set of primers disclosed herein. Optimised conditions for the PCR also contribute to the advantages observed. Such kits and methods may be of benefit in the context of forensic sciences.
    Type: Grant
    Filed: December 24, 2014
    Date of Patent: October 29, 2019
    Assignee: University of Central Lancashire
    Inventors: Sibte Hadi, Rashed Alghafri
  • Patent number: 10442862
    Abstract: The present invention relates to methods for treating gastric neoplasias, in particular treating patients who have been previously determined to have an EGFR biomarker. Gastric carcinoma (GC) is one of the most common and deadest cancers with ?1 million diagnoses and ?0.7 million deaths each year worldwide, with high incidence in Eastern Asia.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: October 15, 2019
    Assignee: Crown Bioscience, Inc. (Taicang)
    Inventors: Jie Yang, Yiyou Chen, Henry Qixiang Li, Jie Cai
  • Patent number: 10400277
    Abstract: The invention relates to a method for enriching a target polynucleotide sequence containing a genetic variation said method comprising: (a) providing two primers targeted to said target polynucleotide sequence; (b) providing a target specific xenonucleic acid clamp oligomer specific for a wildtype polynucleotide sequence; (c) generating multiple amplicons using PCR under specific temperature cycling conditions; and (d) detecting said amplicons.
    Type: Grant
    Filed: August 10, 2015
    Date of Patent: September 3, 2019
    Assignee: DIACARTA LTD
    Inventors: Michael J Powell, Aiguo Zhang
  • Patent number: 10383956
    Abstract: [Problem] To provide a novel fluorescent probe for dipeptidyl peptidase IV, and a detection method and a detection kit using the fluorescent probe. [Solution] A fluorescent probe for detecting dipeptidyl peptidase IV (DPP-IV), said probe comprising a compound represented by formula (I) or a salt thereof. In formula (I): A and B are either the same or different and independently represent an amino acid residue, provided that A is bonded via an amide bond to NH in an adjacent formula and B is bonded via an amide bond to A; R1 represents hydrogen atom(s) or 1 to 4 substituents bonded to the benzene ring, said substituents being either the same or different; R2, R3, R4, R5, R6 and R7 independently represent a hydrogen atom, a hydroxyl group, an alkyl group or a halogen atom; R8 and R9 independently represent a hydrogen atom or an alkyl group; and X represents a C1-C3 alkylene group.
    Type: Grant
    Filed: July 10, 2015
    Date of Patent: August 20, 2019
    Assignee: The University of Tokyo
    Inventors: Yasuteru Urano, Yasuyuki Seto, Haruna Onoyama, Yugo Kuriki, Mako Kamiya
  • Patent number: 10364458
    Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.
    Type: Grant
    Filed: July 16, 2015
    Date of Patent: July 30, 2019
    Assignee: Tangen Biosciences, Inc.
    Inventors: John R. Nobile, John Davidson
  • Patent number: 10358660
    Abstract: This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions, e.g., consortia, that are both specifically optimized to stimulate methanogenesis, or for “methylotrophic” or other conversions. In alternative embodiments, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In other embodiments, the invention provides methods for simulating biogas in sub-surface conditions using a computational model.
    Type: Grant
    Filed: January 3, 2018
    Date of Patent: July 23, 2019
    Assignee: TAXON BIOSCIENCES, INC.
    Inventors: Matthew Ashby, Ladonna Wood, Ulrika Lidstrom, Christine Clarke, Alison Gould, Dariusz Strapoc, Adewale J. Lambo, Bradley James Huizinga
  • Patent number: 10337061
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: July 2, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • Patent number: 10329608
    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: June 25, 2019
    Assignee: Life Technologies Corporation
    Inventors: Earl Hubbell, Christian Koller, Nils Homer
  • Patent number: 10316365
    Abstract: The present invention relates, in general, to gene expression profiles, and in particular, to a peripheral blood gene expression profile of an environmental exposure, ionizing radiation. The invention further relates to methods of screening patients for radiation exposure based on gene expression profiling and to kits suitable for use in such methods.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: June 11, 2019
    Assignee: Duke University
    Inventors: John Chute, Joseph Nevins, Holly Dressman, Nelson Chao
  • Patent number: 10280410
    Abstract: The disclosure relates to a cytoplasmic protein complex comprising: (a) a first recombinant fusion protein comprising a kinase, fused to a first interaction polypeptide; and (b) a second recombinant fusion protein comprising a domain comprising a reporter phosphorylation site, whereby the domain is fused to a second interaction polypeptide. The disclosure relates further to a method to detect compound-compound-interaction using the cytoplasmic protein complex, and to cells comprising such cytoplasmic protein complex.
    Type: Grant
    Filed: February 29, 2012
    Date of Patent: May 7, 2019
    Assignees: UNIVERSITEIT GENT, VIB VZW
    Inventors: Jan Tavernier, Samuel Lievens
  • Patent number: 10254290
    Abstract: A device for studying protein conformation transformation can include a macroscopic substrate, and chaperonin proteins bound to the substrate, each chaperonin protein being capable of binding to a protein of interest during or after undergoing protein conformation transformation. The device may also include the proteins of interest bound to the substrate, where the substrate is included in a label-free assay system. A method of studying protein conformation transformation can include: providing a macroscopic substrate bound with the chaperonin protein and immersing the chaperonin protein in a study composition having the protein of interest, or include providing a macroscopic substrate bound with the protein of interest; and immersing the protein in a study composition having the chaperonin. Such a method can be done with and without a potential stabilizer in order to determine whether the potential stabilizer stabilizes the protein of interest.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: April 9, 2019
    Assignee: The University of Kansas
    Inventors: Mark T. Fisher, Subhashchandra Naik
  • Patent number: 10240199
    Abstract: Methods are provided for diagnosing pregnancy-associated disorders, determining allelic ratios, determining maternal or fetal contributions to circulating transcripts, and/or identifying maternal or fetal markers using a sample from a pregnant female subject. Also provided is use of a gene for diagnosing a pregnancy-associated disorder in a pregnant female subject.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: March 26, 2019
    Assignee: The Chinese University of Hong Kong
    Inventors: Yuk-Ming Dennis Lo, Rossa Wai Kwun Chiu, Kwan Chee Chan, Peiyong Jiang, Bo Yin Tsui
  • Patent number: 10126310
    Abstract: The present invention relates to methods for determining a binding and/or functional interaction of a protein of interest with a nucleosomal substrate wherein at least one of the histone types of the nucleosomal substrate has a homogenous post-translational modification pattern. Further, the invention relates to nucleosomal substrates, wherein at least one of the histone types of the nucleosomal substrate has a homogenous post-translational modification pattern, and to methods for providing such nucleosomal substrates.
    Type: Grant
    Filed: January 13, 2015
    Date of Patent: November 13, 2018
    Assignee: PROTEROS BIOSTRUCTURES GMBH
    Inventors: Adrian Schomburg, Robert Van Der Burgh
  • Patent number: 10100354
    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: October 16, 2018
    Assignee: Life Technologies Corporation
    Inventors: John Leamon, Mark Andersen, Michael Thornton
  • Patent number: 10103015
    Abstract: Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which a desorption solvent utilized in a sampling interface to desorb one or more analyte species from an SPME device is fluidly coupled to an ion source for ionizing the one or more analyte species desorbed into the desorption solvent for subsequent mass spectrometric analysis (e.g., without a liquid chromatography (LC) column between the sampling interface and the ion source).
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: October 16, 2018
    Assignees: DH Technologies Development Pte. Ltd.
    Inventors: Don W. Arnold, Thomas R. Covey, German Augusto Gomez Rios, Chang Liu, Janusz B. Pawliszyn
  • Patent number: 10081830
    Abstract: [Object] A method of detecting nucleic acids easily without requiring complicated operations such as mixing of liquids and cleaning within micro-scale flow channels. [Solving Means]A method of detecting nucleic acids including the steps of bringing a sample containing the nucleic acids into contact with copper, and detecting fluorescence emitted from the sample is provided. According to the method of detecting nucleic acids, only by bringing the sample containing nucleic acids into contact with copper, the fluorescence derived from the composite of the nucleic acids and copper can be easily detected.
    Type: Grant
    Filed: September 6, 2016
    Date of Patent: September 25, 2018
    Assignee: SONY CORPORATION
    Inventor: Nao Nitta
  • Patent number: 10011862
    Abstract: The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more methylated or unmethylated target nucleotide sequences in the sample is identified based on the detection.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: July 3, 2018
    Assignee: Cornell University
    Inventors: Francis Barany, Eugene Spier
  • Patent number: 9975923
    Abstract: The present invention provides methods and compositions comprising a chimeric norovirus capsid protein comprising a norovirus VP1 major capsid protein backbone comprising a norovirus epitope selected from the group consisting of: a) Epitope A; b) Epitope B; c) Epitope C; d) Epitope D; e) Epitope E; f) Epitope F; and g) any combination of (a) through (f) above, wherein the norovirus epitope is from a norovirus strain that is different from the norovirus VP1 major capsid protein backbone.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: May 22, 2018
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Ralph Steven Baric, Lisa Chon Lindesmith, Kari Moore Debbink, Eric Francis Donaldson, Jesica Anne Swanstrom
  • Patent number: 9976173
    Abstract: This invention pertains to devices, compositions and methods that can be used for the rapid determination of microorganisms, cells and other analytes of interest (e.g. a nucleic acid target) as well as associated properties of said microorganisms, cells and analytes. For example, said devices, compositions and/or methods can be applied to the determination of a trait of a microorganism present in a sample. Said devices, compositions and methods utilize matrix-forming prolonged-dissolution hydrophilic polymer to encapsulate hybridization probes and optionally other assay reagents within two or more reagent zones and/or matrix zones disposed on the surface of a substrate. Each reagent zone and/or matrix zone can be designed as a separate assay. Thus, a plurality of assays can be performed on a single substrate. In some embodiments, devices can be supplied in a form ready for a customer to rapidly perform one or a plurality of assays.
    Type: Grant
    Filed: October 9, 2013
    Date of Patent: May 22, 2018
    Assignee: ADVANDX, INC.
    Inventors: Martin Fuchs, Michelle C. Meltzer, Melissa K Deck