Method Of Screening A Library Patents (Class 506/7)
  • Patent number: 11608527
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: April 13, 2022
    Date of Patent: March 21, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Patent number: 11561218
    Abstract: The invention relates to a method for detecting a dengue infection in a patient blood sample, comprising the steps: a) Performing an analysis of prespecified parameters of blood platelets and prespecified types of blood cells in the sample and determining parameter values for the prespecified parameters of the platelets and the prespecified types of cells; b) Obtaining sample parameters from the values determined in step a); and c) Evaluating the sample parameters in relation to a prespecified criterion, wherein, if the criterion is fulfilled, a dengue infection is present.
    Type: Grant
    Filed: March 8, 2019
    Date of Patent: January 24, 2023
    Assignee: Siemens Healthcare Diagnostics he.
    Inventors: Kieran O'Donoghue, Rory Sobolewski
  • Patent number: 11554370
    Abstract: Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.
    Type: Grant
    Filed: April 7, 2022
    Date of Patent: January 17, 2023
    Assignee: Cellanome, Inc.
    Inventors: Tarun Kumar Khurana, Ali Agah, Yir-Shyuan Wu, Filiz Gorpe Yasar
  • Patent number: 11534392
    Abstract: A cosmetic composition including exosomes derived from Galactomyces as an active ingredient is provided for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition has excellent effects on skin regeneration, skin elasticity improvement and/or skin wrinkle reduction.
    Type: Grant
    Filed: February 9, 2021
    Date of Patent: December 27, 2022
    Assignee: ExoCoBio Inc.
    Inventors: Yong Weon Yi, Byong Seung Cho
  • Patent number: 11534758
    Abstract: A micro-total analysis system and a method thereof are provided. The micro-total analysis system includes: a microfluidic device, configured to accommodate a liquid to be detected; an optical unit, configured to form a first light irradiated to the microfluidic device; and a detection unit, configured to detect the liquid to be detected and output a detection signal to obtain detection information.
    Type: Grant
    Filed: August 16, 2018
    Date of Patent: December 27, 2022
    Assignee: BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Xue Dong, Yanling Han, Haisheng Wang, Chuncheng Che, Xiaoliang Ding, Yingming Liu, Yuzhen Guo, Wanxian Xu, Peizhi Cai, Haochen Cui, Fengchun Pang
  • Patent number: 11536730
    Abstract: The invention relates to methods for determining whether a subject is afflicted with a motor neuron disease, the method comprising conducting an analysis of cerebrospinal fluid and/or plasma, measuring the level of one or more sterol/oxysterol analytes, and comparing these to reference values. Further, the invention relates to methods of identifying agents suitable for the treatment of MND, and monitoring the progress of the disease.
    Type: Grant
    Filed: July 4, 2017
    Date of Patent: December 27, 2022
    Assignee: Swansea University
    Inventors: William Griffiths, Peter Crick, Yuqin Wang, Jens Kuhle, Andreas Sailer, Juan Zhang, Martin Turner
  • Patent number: 11511277
    Abstract: Provided are a microfluidic apparatus, a method and system for introducing a substance into a cell. The microfluidic apparatus includes a cavity channel, a bulk wave generating device and a surface acoustic wave generating device; a microstructure is arranged on an inner wall of the cavity channel, and the microstructure is constructed for forming a bubble by a solution at the microstructure when the solution is injected into the cavity channel; the bulk wave generating device is configured to generate a bulk wave, the bulk wave enables the bubble to resonate for generating a flow field; and the surface acoustic wave generating device is configured to generate a surface acoustic wave and control a position of at least one particle in the solution.
    Type: Grant
    Filed: January 3, 2017
    Date of Patent: November 29, 2022
    Assignee: SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Hairong Zheng, Wei Zhou, Long Meng, Lili Niu, Zhengrong Lin, Kaiyue Wang, Xiaowei Huang
  • Patent number: 11506672
    Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more of Follistatin-related protein 3, Basigin, Cathepsin B, and Tenascin as diagnostic and prognostic bio-marker assays in renal injuries.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: November 22, 2022
    Assignee: Astute Medical, Inc.
    Inventors: Joseph Anderberg, Paul McPherson, Jeff Gray, Kevin Nakamura, James Patrick Kampf, Thomas Kwan
  • Patent number: 11465144
    Abstract: In one aspect of the invention, the fluidic device includes a fluidic chip includes a body having a first surface and an opposite, second surface, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a fluidic chip registration means formed on the first surface for aligning the fluidic chip with a support structure; and an actuator configured to engage with the one or more channels at the second surface of the body for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flowrates.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: October 11, 2022
    Assignee: VANDERBILT UNIVERSITY
    Inventors: David K. Schaffer, Dmitry A. Markov, Ronald S. Reiserer, Lisa McCawley, Michael Geuy, Clayton M. Britt, John P. Wikswo
  • Patent number: 11454629
    Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
    Type: Grant
    Filed: June 22, 2020
    Date of Patent: September 27, 2022
    Assignee: Berkeley Lights, Inc.
    Inventors: Kristin G. Beaumont, Peter J. Beemiller, Volker L. S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran
  • Patent number: 11450121
    Abstract: An optical readout method for detecting a precipitate (e.g., a precipitate generated from the LAMP reaction) contained within a droplet includes generating a plurality of droplets, at least some which have a precipitate contained therein. The droplets are imaged using a brightfield imaging device. The image is subject to image processing using image processing software executed on a computing device. Image processing isolates individual droplets in the image and performs feature detection within the isolated droplets. Keypoints and information related thereto are extracted from the detected features within the isolated droplets. The keypoints are subject to a clustering operation to generate a plurality of visual “words.” The word frequency obtained for each droplet is input into a trained machine learning droplet classifier, wherein the trained machine learning droplet classifier classifies each droplet as positive for the precipitate or negative for the precipitate.
    Type: Grant
    Filed: June 26, 2018
    Date of Patent: September 20, 2022
    Assignee: The Regents of the University of California
    Inventors: Dino Di Carlo, Aydogan Ozcan, Omai B. Garner, Hector E. Munoz, Carson Riche
  • Patent number: 11446662
    Abstract: A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: September 20, 2022
    Assignee: HITACHI, LTD.
    Inventors: Tomoyuki Sakai, Masataka Shirai
  • Patent number: 11435370
    Abstract: A sample group forming section 24 classifies samples derived from microorganisms into groups according to empirical information showing the species or strain of each sample. A differential analysis section 27 performs a differential analysis using a peak matrix created based on the result of the grouping. An operator enters group rearrangement conditions concerning the drug resistance of microorganisms. Under the entered conditions, a sample group rearranging/rearrangement-cancelling section 25 rearranges the already formed groups by selecting or merging groups using another kind of previously registered empirical information which shows the drug resistance of each group. The differential analysis section 27 performs a differential analysis using a peak matrix newly created based on the result of the rearrangement of the groups. Thus, differential analysis results concerning the resistance to different drugs can be sequentially acquired as the group rearrangement condition is successively changed.
    Type: Grant
    Filed: November 9, 2017
    Date of Patent: September 6, 2022
    Assignee: SHIMADZU CORPORATION
    Inventors: Yoshihiro Yamada, Koretsugu Ogata, Hiroto Tamura, Teruyo Kato
  • Patent number: 11414712
    Abstract: Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: August 16, 2022
    Assignee: HELIXBIND, INC.
    Inventors: Alon Singer, Ranjit Prakash, Srinivas Rapireddy, Jork Nolling
  • Patent number: 11408031
    Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: August 9, 2022
    Assignee: Natera, Inc.
    Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko, Bernhard Zimmerman
  • Patent number: 11345960
    Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: May 31, 2022
    Assignee: Natera, Inc.
    Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko, Bernhard Zimmerman
  • Patent number: 11302416
    Abstract: Systems and methods are disclosed to detect single-nucleotide variations (SNVs) from somatic sources in a cell-free biological sample of a subject by generating training data with class labels; in computer memory, generating a machine learning unit comprising one output for each of adenine (A), cytosine (C), guanine (G), and thymine (T) calls; training the machine learning unit; and applying the machine learning unit to detect the SNVs from somatic sources in the cell-free biological sample of the subject, wherein the cell-free biological sample comprises a mixture of nucleic acid molecules from somatic and germline sources.
    Type: Grant
    Filed: September 1, 2016
    Date of Patent: April 12, 2022
    Assignee: Guardant Health
    Inventors: Bahram Ghaffarzadeh Kermani, Helmy Eltoukhy
  • Patent number: 11299789
    Abstract: A method and system are disclosed for detecting microbial pathogens in a sample suspected of containing the pathogens. The method includes combining loop-mediated isothermal amplification (LAMP) reagents and a polymer gel, such as a hydrogel, together with the sample to form a mixture. The gel polymerizes over a short time to immobilize the viral particles within the mixture. If target DNA/RNA are present in the sample, amplicons are produced. The target microorganisms are detected by visually detecting the presence or absence of the amplicons. The target microorganism concentrations may be determined based on the number of fluorescent amplicon dots after the reaction using a smartphone or a fluorescent microscope. The method may be employed for rapidly and inexpensively quantifying microbial pathogens in environmental water samples with high sensitivity.
    Type: Grant
    Filed: December 31, 2018
    Date of Patent: April 12, 2022
    Assignee: California Institute of Technology
    Inventors: Xiao Huang, Michael R. Hoffmann
  • Patent number: 11279924
    Abstract: A method of preparing a conditionally active polypeptide from a parent polypeptide, comprising steps of evolving a DNA encoding the parent polypeptide by increasing a net charge of the parent polypeptide using one or more techniques selected from increasing a total number of codons of charged amino acid residues in the DNA and decreasing a total number of codons of uncharged amino acid residues in the DNA to create mutant DNAs; expressing the mutant DNAs to obtain mutant polypeptides; and selecting the conditionally active polypeptide from the mutant polypeptides which exhibits a decrease in activity in a first assay at a first value of a condition compared to the same activity in a second assay at a second value of the same condition. The conditionally active polypeptide, pharmaceutical compositions containing same, nanoparticle and drug conjugates thereof and uses thereof are also provided.
    Type: Grant
    Filed: August 22, 2017
    Date of Patent: March 22, 2022
    Assignee: BioAtla, Inc.
    Inventors: Jay M. Short, Hwai Wen Chang, Gerhard Frey
  • Patent number: 11249090
    Abstract: The invention relates to a method of predicting therapeutic responses to TNF blockers before anti-TNF therapy comprising analyzing immune parameters to selected stimuli in patients before therapy and its use for anti-TNF therapy. The invention relates also to a method of determining a predictive biomarker of response to anti-TNF therapy and to the use of the predictive biomarker obtained by the method.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: February 15, 2022
    Assignees: INSTITUT PASTEUR, ASSISTANCE PUBLIQUE—HOPITAUX DE PARIS, UNIVERSITE DE PARIS
    Inventors: Lars Rogge, Silvia Menegatti, Maxime Dougados, Elisabetta Bianchi
  • Patent number: 11237169
    Abstract: Compositions, methods and kits are described for identifying biomolecules (e.g., proteins and nucleic acids) expressed in a biological sample that are associated with the presence, development, or progression of a disease (such as cancer), or more generally determination of the etiology or risk factors associated with a disease. Sample types analyzed by the disclosed methods include but are not limited to archival tissue blocks that have been preserved in a fixative, tissue biopsy samples, tissue microarrays, and so forth. The methods disclosed herein correlate expression profiles of biomolecules with various disease types, and allow for the determination of relative survival rates; in some embodiments, the methods permit determination of survival rates for a subject with cancer. In other embodiments, the disclosure relates to methods for evaluating therapeutic regimes for the treatment, such as treatment of cancer.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: February 1, 2022
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Stephen M. Hewitt, Joon-Yong Chung
  • Patent number: 11207685
    Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.
    Type: Grant
    Filed: February 12, 2018
    Date of Patent: December 28, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Thomas Henry Cauley, III, John Dzenitis, Steve Hobbs, Stefano Schiaffino
  • Patent number: 11206138
    Abstract: An apparatus includes a tester to detect a biological signature of a biological sample, a processor, and a memory operably coupled to the processor. The memory stores instructions to cause the processor to receive an indication of the biological signature from the tester, and to generate, using a smart contract and through communication with a distributed ledger, a cryptographic token including a digital identifier based on the biological signature. The cryptographic token is transmitted to a remote processor for verification of the biological sample, in response to receiving the cryptographic token. The tester can detect the biological signature within a predetermined test duration that is less than a DNA sequencing duration associated with the biological sample, and the biological signature has a data precision sufficient to uniquely identify the biological sample from a plurality of biological samples.
    Type: Grant
    Filed: October 16, 2019
    Date of Patent: December 21, 2021
    Assignees: Ernst & Young U.S. LLP, EYGS LLP
    Inventors: James C. Canterbury, Shyam Bharat Khatau, Parvez Hakim
  • Patent number: 11198912
    Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of lung cancer. A subject can be screened for lung cancer based on expression of specific mRNAs, miRNAs or the detection of a nucleic acid, protein, peptide or other biological molecule in blood, serum or plasma. Embodiments include 29 specific miRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer, along with 81 additional nucleic acids, proteins or peptides for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of lung cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with the disease.
    Type: Grant
    Filed: August 26, 2020
    Date of Patent: December 14, 2021
    Assignee: Liquid Lung DX
    Inventors: Patrick Lilley, Martin J. Keiser, III
  • Patent number: 11142793
    Abstract: Sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising two electrodes separated by an insulating layer are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the insulating region. A sequencing reaction mixture comprising nucleotide analogs having impedance labels is introduced to the array of nanoscale electronic elements under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance.
    Type: Grant
    Filed: October 30, 2019
    Date of Patent: October 12, 2021
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Stephen Turner, Jeremiah Hanes, Keith Bjornson
  • Patent number: 11111531
    Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: September 7, 2021
    Assignee: TANGEN BIOSCIENCES, INC.
    Inventors: John R. Nobile, John Davidson
  • Patent number: 11104950
    Abstract: Panels of 8-, 9- and 12-biomarker for diagnostic and prognostic methods to determine a subject's radiation exposure and discriminates between persons who have been exposed to radiation only, inflammation stress only, or a combination of the two.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: August 31, 2021
    Assignee: The Regents of the University of California
    Inventors: Andrew J. Wyrobek, Antoine M. Snijders
  • Patent number: 11097247
    Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: August 24, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Nicholas Heredia
  • Patent number: 11078543
    Abstract: Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: August 3, 2021
    Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
  • Patent number: 11072826
    Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: July 27, 2021
    Assignee: Natera, Inc.
    Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko
  • Patent number: 11061045
    Abstract: A matrix droplet extruder includes one or a plurality of reagent containers. Pneumatic connectors are each connectable to a corresponding docking station connector of a docking station that is connectable to a pneumatic generator controllable by a controller to provide pneumatic pressure to the pneumatic docking station connectors. A droplet matrix extrusion surface includes an array of perforations. A liquid management chip has a network of dispensing channels for dispensing reagents from reagent containers through the array of perforations. A pneumatic control network includes pneumatic channels and gates that are controllable by application of the pneumatic pressure to the gates via the pneumatic channels to enable or block dispensing the one or more reagents to repeatedly generate a matrix of droplets when applying the pneumatic pressure to the reagents.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: July 13, 2021
    Assignee: Picodya Technologies Ltd.
    Inventor: Yehuda Yavets-Chen
  • Patent number: 11047013
    Abstract: Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: June 29, 2021
    Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
  • Patent number: 11021757
    Abstract: There is a need for improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer, especially lymphoid neoplasms, such as lymphomas and leukemias. Provided herein are methods for using DNA sequencing to identify personalized, or patient-specific biomarkers in patients with lymphoid neoplasms, autoimmune disease and other conditions. Identified biomarkers can be used to determine and/or monitor the disease state for a subject with an associated lymphoid disorder or autoimmune disease or other condition. In particular, the invention provides a sensitive method for monitoring lymphoid neoplasms that undergo clonal evolutions without the need to development alternative assays for the evolved or mutated clones serving as patient-specific biomarkers.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: June 1, 2021
    Assignee: Adaptive Biotechnologies Corporation
    Inventors: Malek Faham, Thomas Willis
  • Patent number: 11016082
    Abstract: Provided is a non-invasive method of detecting or screening for a cancer in a subject specimen originating in a tissue outside of the lung. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for the cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for the cancer in the subject specimen.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: May 25, 2021
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Xiaoan Fu, Michael Nantz, Michael Bousamra, Victor van Berkel
  • Patent number: 10994275
    Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: May 4, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Michael Pallas, Evan Foster, Jorge Fonseca, James Nurse, Theodore Straub
  • Patent number: 10961583
    Abstract: The disclosure provides methods of identifying a human subject as a candidate for treating or inhibiting a liver disease by inhibiting HSD17B13. The disclosure also provides methods of treating a subject who is PNPLA3 Ile148Met+ by administering an inhibitor of HSD17B13. The disclosure also provides method of detecting a PNPLA3 Ile148Met variant and functional HSD17B13 in a subject. The disclosure also provides method of identifying a subject having a protective effect against liver disease. The disclosure also provides inhibitors of HSD17B13 for use in the treatment of a liver disease.
    Type: Grant
    Filed: October 11, 2018
    Date of Patent: March 30, 2021
    Assignee: Regeneron Phramaceuticals, Inc.
    Inventors: Yurong Xin, Jesper Gromada, Xiping Cheng, Frederick Dewey, Tanya Teslovich Dostal, Claudia Schurmann, Aris Baras, Noura Abul-Husn
  • Patent number: 10964409
    Abstract: Provided herein are methods, processes, systems and machines for non-invasive assessment of genetic variations.
    Type: Grant
    Filed: October 2, 2014
    Date of Patent: March 30, 2021
    Assignee: Sequenom, Inc.
    Inventor: Gregory Hannum
  • Patent number: 10956806
    Abstract: A computer-implemented method for efficient assembly of oligonucleotides for nucleic acid based data storage includes receiving encoded data including binary data encoded into nucleic acid sequence data, and assembling a target nucleic acid data strand based on the encoded data by, concatenating one or more selected codeword oligonucleotides obtained from a codeword stack strand.
    Type: Grant
    Filed: June 10, 2019
    Date of Patent: March 23, 2021
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Koji Masuda, Eiji Nakamura
  • Patent number: 10907196
    Abstract: The present application relates to new coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: January 9, 2020
    Date of Patent: February 2, 2021
    Assignee: Illumina Cambridge Limited
    Inventor: Nikolai Nikolaevich Romanov
  • Patent number: 10878703
    Abstract: The present invention provides agents comprising or consisting of a binding moiety with specificity for interleukin-1 receptor accessory protein (IL1RAP) for use in inducing cell death and/or inhibiting the growth and/or proliferation of pathological stem cells and/or progenitor cells associated with a neoplastic hematologic disorder, wherein the cells express IL1RAP. A related aspect of the invention provides agents comprising or consisting of a binding moiety with specificity for interleukin-1 receptor accessory protein (IL1RAP) for use in detecting pathological stem cells and/or progenitor cells associated with a neoplastic hematologic disorder, wherein the cells express IL1RAP. Further provided are pharmacological compositions comprising the agents of the invention and methods of using the same.
    Type: Grant
    Filed: May 21, 2018
    Date of Patent: December 29, 2020
    Assignee: Cantargia AB
    Inventors: Thoas Fioretos, Marcus Järås
  • Patent number: 10794898
    Abstract: Methods for identifying a compound that alters fluorescence resonance energy transfer (FRET) of a protein. The methods include use of a genetically engineered cell that includes a target protein. The target protein includes one or more heterologous domains. In one embodiment, a target protein includes two heterologous domains, and in another embodiment, the target protein includes a heterologous domain and the cell further includes a second protein that includes a heterologous domain. A heterologous domain may include a chromophore or an amino acid to which a fluorescent dye attaches. The fluorescence lifetime of one or more chromophore, one or more fluorescent dye, or the combination thereof, is measured after contacting the cell with a compound A difference between the fluorescence lifetime in the presence of the test compound and the fluorescence lifetime in the absence of the test compound indicates that the test compound alters the FRET of the target protein.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: October 6, 2020
    Assignees: Regents of the University of Minnesota, Loyola University Chicago
    Inventors: David D. Thomas, Simon Joseph Gruber, Razvan L. Cornea, Gregory David Gillispie, Kurt C. Peterson, Seth Louis Robia
  • Patent number: 10774370
    Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: September 15, 2020
    Assignee: 10X Genomics, Inc.
    Inventors: Xinying Zheng, Serge Saxonov, Michael Schnall-Levin, Kevin Ness, Rajiv Bharadwaj
  • Patent number: 10746738
    Abstract: The present invention is directed to claudin-1-specific peptide reagents, methods for detecting pre-cancer (dysplasia), early cancer and/or cancer using the peptide reagents, and methods for targeting pre-cancerous (dysplastic) cells, and/or cancer cells using the peptide reagents.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: August 18, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Thomas D. Wang, Emily Rabinsky, Bishnu P. Joshi
  • Patent number: 10730030
    Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Nicholas Heredia
  • Patent number: 10734117
    Abstract: Apparatuses (including devices and systems) and methods for determining if a patient will respond to a variety of cancer drugs.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: August 4, 2020
    Assignee: STRAND LIFE SCIENCES PRIVATE LIMITED
    Inventors: Vaijayanti Gupta, Manimala Sen, Satish Sankaran, Kalyanasundaram Subramanian, Ramesh Hariharan, Vamsi Veeramachaneni, Shanmukh Katragadda, Rohit Gupta, Radhakrishna Bettadapura, Anand Janakiraman, Arunabha Ghosh, Smita Agrawal, Sujaya Srinivasan, Bhupender Singh, Urvashi Bahadur, Shuba Krishna, Mahesh Nagarajan, Nimisha Gupta, Sudhir Borgonha
  • Patent number: 10724092
    Abstract: The disclosure provides method and kits for characterizing spliced m RNA isoforms. The disclosure also provides methods of screening for mutations and oligonucleotides that modulate splicing.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: July 28, 2020
    Assignee: Erasmus University Medical Center Rotterdam
    Inventors: Atze Jacobus Bergsma, Erik van der Wal, Wilhelmus Wenceslaus Matthias Pijnappel, Antje Tjitske van der Ploeg, Arnoldus Reuser
  • Patent number: 10725382
    Abstract: The present invention provides a system for conservation and efficient use of energy through controlling and monitoring of devices. At least one processing controller connected to a sensor and a device, the processing controller configured to receive the ambient data from the sensor and operating parameters from the device; a user module configured to IO receive input parameters from a plurality of users; a central processing module, connected to the structure, the user module, and the admin module through wired and/or wireless connection, the central processing module configured to process the data received from the processing controller adapted in the zone of the structure and generate the optimum parameters for operating the device adapted in the zone to the structure.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: July 28, 2020
    Assignee: Saint Louis University
    Inventors: Silviya Petrova Zustiak, Naveed Zafar Ahmed
  • Patent number: 10676776
    Abstract: A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.
    Type: Grant
    Filed: August 3, 2015
    Date of Patent: June 9, 2020
    Assignee: QUANTAMATRIX INC.
    Inventors: Sunghoon Kwon, Yong-Gyun Jung, Jung Il Choi, Hun Jong Na
  • Patent number: 10643738
    Abstract: Disclosed herein are methods, systems, and apparatus for detecting microamplifications or microdeletions in the genome of a fetus. In some embodiments, the method comprises receiving sequence tags for each of a plurality of DNA fragments in a biological sample; determining genomic positions for the sequence tags; determining whether the density of DNA in each of a plurality of genomic regions is aberrantly high or low; identifying as a microamplification a set of consecutive genomic regions having aberrantly high density; and identifying as a microdeletion a set of consecutive genomic regions having aberrantly low density. The biological sample may be a blood sample obtained noninvasively from a female subject pregnant with the fetus.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 5, 2020
    Assignee: The Chinese University of Hong Kong
    Inventors: Yuk Ming Dennis Lo, Kwan Chee Chan, Peiyong Jiang, Cheuk Yin Jandy Yu, Rossa Wai Kwun Chiu
  • Patent number: 10619206
    Abstract: Described herein are improved methods, compositions and kits for next generation sequencing (NGS). The methods, compositions and kits described herein enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (which can comprise regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information can be obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein can be useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: April 14, 2020
    Assignee: Tecan Genomics
    Inventors: Doug Amorese, Benjamin G. Schroeder, Jonathan Scolnick